No 48 (2012)

Published July 31, 2012

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Articles

Storage of ram semen in gelatin supplemented extender
7-10

The aim of our study was to examine how different gelatin concentrations affect ram semens viability in liquid storage at 5 oC for five days. Our hypothesis was if we add gelatin to the semen extender, than the viability of ram semen will be better in the extenders containing gelatin, than the control. We used two different semen ext...enders:1.5% UHT milk and 1.5% UHT milk + 5% egg yolk. We added 0; 0.5; 1.0; 1.5; 2.0% Dr. Oetker gelatin to the semen extenders. We stored the semen for five days at 5 oC and in every 24 hour we made sampling.
We stained the smears with Kovács-Foote staining and evaluated them with light-microscope. We categorized the cells in five groups like: live and intact cells, live cells with injured acrosome, dead cells, live head with dead tail and live tail with dead head. We used one-way analysis of variance (ANOVA) to assign how gelatin concentration affects the number of the categorized cells. On the fifth day, the viability was the best in the following semen extenders: 1.5% fat UHT milk + 1.0% gelatin and 1.5% fat UHT milk + 1.5% gelatin, but it was not significant (p>0.05).

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Use of essential trace elements enriched Artemia in the larval rearing of barramundi (Lates calcarifer)
11-16

Artemia is a vital nutriment in the rearing of barramundi (Lates carcarifer), however it’s mineral trace element content does not cover the requirements of the larvae. In our experiment the assumption was wether the cobalt, zinc and manganese concentration of Artemia could be increased during a 24 hours of enrichment period, as well as we wan...ted to investigate the resulted interactions between the elements. For this purpose 50, 100 and 1000 mg l-1 cobalt-chloride treatements (Co50, Co100, Co1000) were applied while in the case of 50 and 100 mg l-1 treatments the enrichment was also complemented with zink-sulphate and manganese-chloride in the same concentrations. According to the results the zooplankton were able to accumulate cobalt in higher amount, and yet zinc and manganese had no significant effect on each other, a strong synergistic effect occurred between cobalt and manganese. After the enrichment period the impact of essential trace element supplemented live feed on the survival and growth of barramundi larvae was also studied. The experiment was carried out by using 15 days-old larvae applying a total of 9 treatments for two weeks (Control, Co50, Co100, Mn50, Mn100, CoZn50, CoZn100, CoMn50, CoMn100), in duplicates. Considering the per cent of survivals, no significant difference was observed between the treatements (P>0.05). In the case of CoMn50 and CoMn100, the loss statistically increased (P<0.05) compared to the Control (80.5±4.95%) group. Our results show, that the Mn100 and CoMn100 treatements resulted in  ignificantly higher (P<0.05 ) in divid ual bod y w eight s, how ever taki ng the survival also into consideration the Mn100 treatements proved to be the most
effective.

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Preliminary results of intraspecific sterlet hybrid (Siberian sterlet × sterlet) rearing under intensive conditions
17-19

Due to early maturation and a small size, sterlet (Acipenserrut-henus) is a very advantageous fish for intensive sturgeon farming with regard to caviar production. According to our previous observations, the growth potential of Siberian sterlet (A.ruthenusmarsiglii) is higher than that of the European one. In our experiment, sterlet and an intr...aspecific hybrid (Siberian sterlet × sterlet) were tested under intensive tank conditions. During the 8 weeks of the experiment, survival, growth and size heterogeneity were investigated. According to the results, there were no significant differences in survival and growth but the size heterogeneity was higher in the hybrid. It is supposed that, in our trial, the applied water  temperature range (24.0–25.5 °C) was optimal for sterlet, but was probably above the optimal range for the hybrid.

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Analysis of longevity in Holstein Friesian cattle using proteomic approaches
21-25

The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production li...fe with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,
we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group.

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A partial botanical survey of a gamepreserve
27-30

Hungary is internationally well known for its high level game management. An important part of game management is indoor game keeping. In Hungary more and more game preserves were founded all over the country. The role of these game preserves to retain forest stands outside of the closed area. According to the law, in the game-preserves the gam...e bearing capacity is not the most important parameter, because in these areas the feeding is continuous. In practice it means that in game-preserves we find a much higher density of games than in nature. This causes a relative big loading of the area (of the herbaceous and arboreal), where the game-preserves are located. Our aim is to study the ecological and management issues of forest- and game resources. The scene of these work is a game-preserve related to the Nyírerdő Corporation (Nyíregyháza).
We have done a botanical survey (plant coverage, life-form spectrum) in the three previous units and in the control area (outside of the game-preserve) as well. Our results show the difference in case of the state of herbaceous plants between areas with higher and lower game density.

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Some methodological aspects of animal behaviour during beef cattle grazing
31-35

Ethology, the research field of animal behaviour, during the past half century developed into an independent science and became more important in recent years as the farming industry has turned toward quality production. Farm animals respond for every environmental factor. Essential to know the answers to avoid unpleasant economic consequences.... Based on the shepherds’ experience, this science has merged with modern technology, constantly expanding and searching new methods. According to the literature the article summarizes the observation methods in cattle grazing. This paper introduces the beginnings and shows the future trends. Finally we share personal experiences as the Hungarian Grey cattle grazing behaviour at conditions of Hortobágy, Hungary.

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Organoleptical and instrumental examination of broilers’ valuable meat-parts
37-41

The aim of our study was to compare some meat quality parameters (color,- evenness of colour- and tone of meat) of Ross 308, Cobb 500 and 
Ross parents hibrids. These qualities are of preliminary importance for consumers and are related to several evaluating features influencing the preparation. The colour of the breast and thigh muscle...s was measured by Minolta CR-300 type colour measuring equipment. The evenness of color- and tone of the breast and thigh muscles were measured in the laboratory of Campden BRI Magyarország Nonprofit Kft.
We found that in the females the color of Ross 308 pullets’ breast meat was significantly darker. In the males the darkest breast meat was detectable in the case of the Ross parents hybrids. In neither sexes wast found significant difference – in all but one – in quality parameters of thigh meat.

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The methodology of the Hooded Crow’s (Corvus cornix L.) colour ringing and the previous results
43-48

The Hooded Crow (CorvuscornixL.) have moved in several Hungarian cities in the last few decades. It is breeding in Debrecen since 1959, and nowdays it’s presence can be detected at all points of the city, it is an permanent breeding species of the bird fauna. Our knowledge about the nature of urban races, including motion patterns, area fidel...ity is sufficiently incomplete. The aim of our research is to answer these questions by using our colour-ring program. In this study we present in details the methodology of Hooded Crow’s colour-ring program in urban area, and we also report our previous
achievements.

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Sporthorse performance testing in eventing by own and progeny performance
49-56

The aim of the study was to evaluate the Hungarian Sporthorse population based on eventing competition performance. The database contained the results of 792 horses and 449 riders between 2000 and 2006. The eventing results were gathered from Hungary and other European countries. Blom transformed ranks were used to evaluate the sport performanc...e.Three models were fitted to the Blom scores. Evaluating all the competition categories at the same time weighted Blom scores were used according to the difficulty of the category. The linear mixed model included fixed effects for age, sex, breeder, owner, location, year; and random effects for animal and rider. Horses from the database were judged by their own performance, and stallions were investigated by performance of their progenies on the basis of descriptive statistics of Blom scores and weighted Blom scores. Breeding values of eventing performance were predicted. To improve the reliability of breeding values, more progenies should be
used in eventing competitions. 

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Characteristics of roe deer bot fly infection in the southern part of the Great Hungarian Plain
57-60

I am investigating the occurance of one of the diseases caused by parasites in plain roe deer populations, namely a botfly larvae, Cephenemyiastimulator, (Clark, 1815) ranged among the Oestridae family. Nasal botfly larvae are common roe deer parasites and are generally spread nationwide. The subject of the present survey is the processing of t...he data gained about roe deer bucks of the year 2009 and their infestation indices. I investigated botfly larvae in 90 bucks’ nasal and pharyngeal cavity. We have found botfly larva in 18.9% of the examined bucks (prevalence), this means 17 infected specimen. I collected 218 pieces of larvae from the samples, with a 12.8 pieces average intensity. In my investigations prevalence was lower and mean intensity was higher than in previous Hungarian researches. This was manifested both in my overall data, and in our examinations by the different age groups.

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Genetic diversity study of Hungarian honey bee (Apis mellifera L.) colonies – Previous announcement
61-64

In Hungary, the Apismelliferacarnica is the native breed which is the only recognised and breedable honey bee in the country. It is assumed that there are a number of non-native and hybrid honey bee breeds in Hungary. The microsatellite and mitochondrial DNA surveys applied here will be utilised to easily and accurately identify the various spr...ads, and open up new ways in the research of honey bees. The isolation of the genomial DNS from 5 to 7 day old larvae samples was successfully carried out. In the future the plan is to carry out the measure with microsatellite markers. As an initial step the optimal annealing temperature was identified. In the mitochondrial DNA survey the COI-COII mytochonrial regional primer due to its low anneling temperature cannot be used with any normal sequencing methods. By using these method the aim of this research is the measurement of genetic variance.

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Determination of the validation parameters of inductively coupled plasma mass spectrometer (iCP-mS): response curve linearity in the case of arsenic and selenium
67-71

In the field of elemental analysis inductively coupled plasma mass spectrometers (ICP-MS) have the best sensitivity that means the lowest limit of detection, subsequently their applicability for the detection of essential and toxic elements in foods and foodstuffs is prominent. For the most elements could be measured the detection limit is betw...een μg kg-1 (ppb) and ng kg-1 (ppt) e.g. for arsenic and selenium.

Considering an analytical task (sample type, analytes and their concentration, pretreatment procedure etc.) the applicability of an analytical method is determined by its performance characteristics. The purpose of validation is to ensure that the method would be used fulfills the requirements of the given task. In this article we describes one of the performance characteristics, the linearity, and the whole validation procedure aims measurement of arsenic and selenium in foodstuffs by inductively coupled plasma mass spectrometer (Thermo XSeries I.); but because of the limited number of pages the results are demonstrated only for arsenic.

The linearity of calibration was evaluated in three concentration ranges (0.1–1 μg l-1; 1–10 μg l-1; 10–50 μg l-1), with nine line-fit possibilities (without weighting, weighting with absolute or relative deviation; with or without forcing the curve through blank or origin) and different methods (graphical examination, correlation coefficient, analysis of variance).

The best method to ensure the linearity of correspondence between signal and concentration was the ANOVA test. In view of calibrations it was found that the range of 10–50 μg l-1 could be regarded as linear with four line-fit possibilities, and was non-linear between 0.1–1 μg l-1 and 1–10 μg l-1.

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The effect of different fertilizer treatments on the sulphur and protein content of wheat
73-76

In this study the effect of N,P and K nutrients on the S and protein content of wheat grains was investigated in a long-term fertilization experiment set up in Nagyhörcsök. The calcareous chernozem soil having the following characteristics: pH (KCl): 7.3, CaCO3: 4.27%, humus: 3.45%, Al-soluble P2O5 and K2...O: 60–80 and 180–200, KCl- Mg: 150–180, KCl+EDTA-soluble Mn-, Cu- and Zn-content: 80–150, 2–3 and 1–2 mg kg-1. The experiment had a split-split-plot design with 40 treatments in 4 replications. Plant samples were collected from 2002 and 2004. 2002 was a drought year while 2004 was very wet.
The main conclusions are as follows:
– The sulphur and protein content were than the control higher in every NPK treatments.
– The sulphur and protein content of the wheat grains were higher in 2004 that had a lot of rain than in 2002 that had drouht.

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Comparison of the sample preparation methods worked out for the examination of the element content of wine
77-82

The examination of the potentially toxic elements content of the wines is not easy task, because the most elements are in little concentration (mg kg-1 or μg kg-1) in the wine and the wines contain great amount of organic matrix. The efficient sample preparation is essential for the accurate determination of element conte...nt. The eim of our research was to determine which sample preparation method will be the most efficient in examination of wines with ICP technology. The examined wine sample was a 2008 Chardonnay from the Eger wine region. We did the sample preparation and analysis examination in University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Institute of Food Science, Quality Assurance and Microbiology.
We did the analysis examinations with ICP- MS (inductively coupled plasma mass spectroscopy). We always did the sample preparations and the examinations in three times rehearsal. The applied sample preparation methods: dilution with distilled water, open digestion and microwave digestion. 
We were able to measure B, Al, Mn, Fe and Zn with only dilution and open sample preparation. In the smaller quantity present Sr and Ba were measurable in the wine in the case of all three methods well. We were able to measure the Co with dilution and open digestion method,  while Cr, Ni, and Te with only dilution method. In the case of arsenic we were not able to measure reliable result with dilution and open digestion method because of organic matrix and other components
(alcohols, monosaccharides, polysaccharides, polyalcohols and inorganic salts). On the whole we are able to say that in the case of certain elements (B, Mn, Fe, Zn, Sr, Ba) the open digestion and dilution sample preparation is applicable well, however, in the case of certain elements (As, Al, V, Cr, Se, Mo, Cd, Hg, Pb) we have to develop the methods. It may be development of one of the way, if we develop sample preparation methods to examined element specifically and not
to wine generally.

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The effect of different microbial preparations on some soil characteristics
83-86

In pot experiment the effect of different microbial inoculants and their combinations with NPK fertilizer and wheat straw on some soil properties (physical, chemical, and microbiological parameters) were studied. The experiment was set up in 2011 at the Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random b...lock design. The studied soil type was calcareous chernozem soil from Debrecen (Látókép) with ryegrass (Lolium perenne, L.) test plant.
At the end of the experiment in our laboratory the nitrate-nitrogen content of soil, the AL-soluble phosphorus and potassium content of soil, the urease enzyme activity of soil, the total number of bacteria and the number of microscopical fungi were determined.
The results of the study were the following:
– The straw treatment and the straw + biofertilizer combinations influenced positively the nitrate content of soil.
– The NPK fertilization and the straw+bacterial fertilizer combinations had significant positive effect on the AL-soluble phosphorus content of the soil.
– The biofertilization and the straw+biofertilizer combinations stimulated the AL-soluble potassium content of soil occasionally.
– The total number of bacteria was influenced by the NPK fertilization, the bacterial fertilization and the straw+bacterial fertilizer combinations significantly.
– In case of the number of microscopic fungi caused in some cases significant changes the NPK+bacterial fertilizer and straw+bacterial fertilizer combinations.
– The soil urease enzyme activity was increased in all cases strongly especially by the straw+bacterial preparation combinations.

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The effect of dried herbs on the souring of sunflower oil
87-89

Many herbs have antioxidant activity, which can replace the use of potencionally harmful synthetic antioxidants. Sunflower oil fortified with eight different plants and stored at room temperatur in my experiment. Specified intervals with determination of peroxide value, FFA, iodine value, anisidine value and TOTOX value followed the quality of ...the sunflower oil.

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Examination of Hybrid-specific nutrient supply at corn on chernozem soli
91-95

The effect of increasing fertilizer dosages on the yield of eight different maize hybrids (SY Ondina, NK Kansas, NK Lucius, NK Octet, NK Thermo, SY Flovita, SY Brillio, NX 47279) has been investigated in the crop-year of 2011. According to our results it can be stated that contrarily to the control treatment the application of different nutrien...t-levels has resulted a significant yield increment (2 000–5 800 kg ha-1).
Based upon the results of this experiment we have drawn the conclusion that the nutrient level of 120 kg N+PK was the optimal for the investigated hybrids. The highest yield (14 475 kg ha-1–15 963 kg ha-1) of the hybrids with different genotypes has been produced in case of this fertilizer treatment. With the comparison of the control and the optimum-fertilizer treatments the yield-increasing effect of mineral fertilization and the different reaction of hybrids towards increasing fertilizer dosages have been proven. In case of the control treatments the best-yielding hybrids were NK Thermo (11 917 kg ha-1) and NX 47279 (11 617 kg ha-1). Contrarily on the optimal nutrient supply level the hybrids SY Brillio (15 876 kg ha-1) and NX 47279 (15 963 kg ha-1) have produced the highest yields. Summarizing, we can state that the hybrid NX 47279 has resulted stable and high yields in the fertilized treatments. Analysing the yield-increasing effect of 1 kg fertilizer active substance it was proven, that the hybrids SY Flovita (45.43 kg ha-1), SY Brillio (44.47 kg ha-1) and NX 47279 (42.33 kg ha-1) had a good reaction towards even lower nutrient supply levels as well. In case of the control treatment the average water utilization coefficient of the hybrids was significantly lower (35.2 kg mm-1), than in case of the optimal nutrient supply level (N120+PK) treatments (48.9 kg mm-1).
Therefore the hybrid specific difference between the water utilization of genotypes could be revealed.

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Quality analysis of the near-infrared spectra of wheat samples
97-100

The near infrared spectroscopy is widely used in the different industries as a rapid, non-invasive analitical tool. It is suitable for identification, qualification and quantitative analysis as well. As this technique is indirect, to make accurate calibration equations we need a proper sample population. Before the quantitaive analysis, develop...ing calibiration modells we have to collect and examine the spectra. In our study we examined wheat samples with known origins to find if there is any effect of the growing area on the NIR spectra.

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Effect of NPK fertilization on the yield and yield stability of different maize genotypes
101-104

The yielding capacity and quality parameters of 11 maize hybrids were studied in 2011 on calcareous chernozem soil in a 25-year long-term fertilization experiment in the control (without fertilization), in the base treatment of N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 and in... five treatments which were the multiplied doses of the base treatment. The N fertilizer was applied in the autumn and in the spring, while P and K fertilizers were applied in the autumn.The sowing time was 17–18 April, the time of harvest was 8 October. The 30-year average of precipitation (April–Sept) was 345.1 mm, the amount of precipitation did not differ greatly from that, however, its distribution was very unfavourable.
It was found that the largest yield increment (as compared to the control) was in the treatment N 40 kg ha-1, P2O5 25 kg ha-1, K2O 30 kg ha-1 in the long-term experiment. The largest yields were obtained for the hybrids P9494, PR37N01 and PR35F38 (13.64–13.71 t ha-1). Due to the dry period at the end of the summer – beginning of autumn, the grain moisture content at harvest was favourably low, 12–18% depending on the treatment and the growing season.

The N fertilization significantly increased the protein content of the kernel, but the starch content of the kernel decreased (significantly in several cases) with increasing fertilizer doses and yields as compared with the control.
The highest protein content was measured in hybrids GK Boglár and Szegedi 386. The oil content was above 4% for GK Boglár, but the two hybrids were not among the best yielding hybrids in spite of their good inner content. The starch content was around 75 % without fertilization, it decreased with fertilization.
For the tested hybrids, the fertilizer dose N 120 kg ha-1, P2O5 75 kg ha-1, K2O 90 kg ha-1 can be recommended with respect to efficacy and environmental considerations.

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Study of some physiologic properties of different genotype sweet corn hybrids
105-110

The effect of nutrient-supply (control, N120+PK) and two different genotypes on the physiologic properties of sweet corn has been investigated in the crop-year of 2011 on chernozem soil in the Hajdúság region. The experiments were carried out at the Experimental Station of the University of Debrecen in Debrecen-Látókép. The experiment was ...sewn in two different sowing times: the 21st April can be considered as an early, while the 19th May as a late sowing time. The two involved hybrids were Jumbo and Enterprise. The applied plant density was 65 000 plants per hectare.
Our aim with this experiment was to study the plant production, just as the main affecting factors of its development and dynamics, like nutrient-supply and genotypes. We aimed to study and analyse the relationships between these factors and plant production. In this study following parameters were measured and calculated: photosynthetic activity, chlorophyll-content (SPAD-value), leaf area index (LAI) and leaf area duration (LAD). Regarding the analysis of photosynthetic activity values no obvious relationship between the measured values and the applied hybrids, just as nutrient-supply has been revealed. 
Analysing the SPAD-values it can be stated that the chlorophyll-content of the measured leaves showed an increasing tendency due to the nutrient-supply. The highest values have been measured in the intensive cob development phase of the early sowing time plots.
Regarding the LAI-values we have found significant differences between the fertilizer treatments in both sowing time treatments. In case of the leaf area duration values – that is derived from the LAI values – nutrient-supply has positively affected the duration of the assimilation area.

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Examination of the population density and sowing date of different maize genotypes in the Hajdúság region
111-115

The experiment was carried out 6 km from Debrecen, next to the main road 47 on a homogeneous field on brown forest soil. Five corn hybrids were tested in the trial (DKC 4795, DKC 4995, KWS Kornelius, NK Cobalt, PR37 N01) at three different sowing times (early – 5th April, average – 21st April, late – 10th ...May). At each sowing time, three different plant densities were applied (modest – 58 500 plants ha-1, average –70 200 plants ha-1, high – 82 300 plants ha-1). The agrotechnics applied
in the experiment satisfied the requirements of modern corn cultivation.

In the study, the best yield result was achieved with the early sowing time out of the three examined sowing times (11 315 kg ha-1), which was significantly different (LSD5%=495 kg) from that of the average sowing time (10 690 kg ha-1), however, there was no statistically justifiable difference between the yield results of the early and the late sowing times. There was a significant difference also between the average and late sowing time. Our results indicate that the different sowing times resulted in a different flowering times. Consequently, the stands of early and late sowing time reached this critical stadium of growth under proper climatic circumstances (precipitation: 39 mm and 136 mm, average temperature at flowering: 18.1 oC and 20.3 oC), while flowering in the case of the average sowing time of 21st April was in the first half of July and the average temperature at flowering was warmer (23.2 oC) with only 10 mm precipitation.
In the experiment, the plant density response was also examined. According to the measured data, four of the five hybrids responded badly to the increasing plant density. We found that the plant density of 58 500 plant ha-1 gave the largest yield results (DKC 4995 11 794 kg ha-1 – NK Cobalt 10 998 kg ha-1, average of five hybrids: 11 430 kg ha-1), while the lowest yields were obtained at the plant density of 82 300 plant ha-1 (KWS Kornelius 11 037 kg ha-1 – NK Cobalt 10 019 kg ha-1, average of five hybrids 10 720 kg ha-1). The difference between the two plant densities was significant (LSD5%=494 kg), however, the 70 200 plant ha-1 plant density did not show any statistical difference from neither the 58 500 ha-1 nor from the 82 300 plant ha-1 stands. When examining the data of the hybrids separately, we found that there was a significant difference between the average yield of the lowest and highest plant densities only in the case of three (DKC 4795, DKC 4995, NK
Cobalt) out of the five hybrids (DKC 4795: 11 757 kg ha-1 – 10 857 ha-1 where LSD5% =816 kg; DKC 4995: 11 794 kg ha-1 – 10 738 kg ha-1 where LSD5%=853kg; NK Cobalt: 10 998 kg ha-1 – 10 019 kg ha-1 where LSD5%=630 kg ha-1), while a  significant difference between the second and third plant densities was observed only in one case (DKC 4995: 11 726 kg ha-1 – 10 738 ha-1 where LSD5%=853 kg). In all other cases, there was no statistical difference between the different
plant densities.

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The relationship between gluten proteins and baking quality
117-122

Wheat, one of the most important cereals, is grown on the largest area in Hungary. During hydration of storage proteins of wheat – gliadin and glutenin – the gluten complex is evolved. The gliadin is responsible for the extensibility of gluten complex as well as the glutenin for the strength of gluten. The structure, composition and rheolog...ical properties of gluten proteins influence significantly the baking quality. The gliadin/glutenin ratio and the quality and structure of glutenin fraction play the most important role in evolving gluten complex. Changes in the steps of breadmaking technology also have effect on the quality of product. Several tests proved that the higher glutenin content increases the strength of dough while the higher gliadin content increases the extensibility of dough and decreases maximum resistance to extension. The monomer gliadins play a great part in plasticity of glutenin polymer. The quality of glutenin fraction significantly influences the evolving gluten complex, because of the spiral structure of glutenin which deforms under stress conditions, then the β-spiral structure resumes their original conformation by releasing from stress.
The final quality of product evolves as a result of complex characteristics of wheat proteins, so detailed knowledge on the roles of different protein compounds is the base of the quality oriented product development.

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The response of sunflower hybrids to different plant densities on a chernozem soil
123-128

In our experiment the reaction of six sunflower hybrids of different genotypes (NK Oktava, ES Biba, ES Diagora, ES Ballistic, EGH 8925, PR 64 H 42) towards plant density has been investigated by different fungicide treatments in the crop-year of 2011 on a chernozem soil. 
In the crop-year of 2011 sunflower populations were infected by a... significant Diaporthe helianthi disease. The extent of this infection was significantly enhanced by the increment of plant density. However, regarding the average of the hybrids and plant densities the two times executed fungicide treatment has decreased the infection rate by 22%. The most susceptible hybrid was the ES Biba. Contrarily, the hybrid ECH8925 proved to be the most resistant hybrid towards this
disease according to our results. According to the results of the Pearson’s correlation analysis it has been revealed that stalk breakage and Diaporthe infection stand in a very close (r=0.782**) and middle close (r=0.523**) correlation resp. with plant density. The relationship between fungucude treatments and stalk breakage, just as Diaporthe infection showed to be middle and close respectively. Our results demonstrate the role of stalk and plate diseases (among them Diaporthe) in causing stalk breakage, for we have found a close positive correlation between stalk breakage and Diaporthe infection (r=0.624**) in our analysis.
From the aspect of yield amount the optimal plant density varied between 45 000 and 55 000 plants per hectare. Fungucude treatments enabled not only the use of higher plants densities, but they had a yield increasing effect as well. In the crop-year of 2011 the highest yield (4 559 kg ha-1) on a chernozem soil has been measured in case of the hybrid ECH8925.

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The factors affecting the proliferation of mould fungi and mycotoxin production during the storage of wheat and the identification methods of the appearing branches
129-133

Nowadays, it is often suggested that, we should eat products made with whole grain cereals, despite of the fact that it raises the risk of consuming wheat products infected by mold and their toxins originated from the plough-lands and the stocks.
Two third of the cultivated fields in Hungary are planted with cereals. The most alarming proble...m for food and feed security is caused by the Fusarium species. The greatest problem of all is caused by the mycotoxins. When they get into the food chain they can be a serious threat to public health. In addition, we have to face up to the problem of the effects of global warming that influence the growth of microbial infections in different ways.
In this article we tried to summarize the effect of climate change on molds, the factors which have effect on growing and mycotoxin producing of molds and the identification methods of molds.

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Compost application in integrated and organic fruit cultivation
135-139

Nowadays the success vegetable and fruit production are unimaginable without regular nutrient management. One of the ways to supply the required nutrients in an environmentally friendly way is the application of composts, which is less widespread so far. Compost doses were applied in biological and integrated apple orchards in cooperation with ...the Institute of Horticultural Science in the years 2010 and 2011. Different changes were resulted by the compost treatments in the examined parameters in case of both apple varieties (Golden Delicious and Pinova). There is no clear effect of compost on the changes of ash-, total acids, Vitamin C and the sugar content until now, because the improvement of the nutritional indicators takes more time. However significant differences were observed after one year between the apple varieties. Higher ash-, total acids and sugar content were measured in case of the apple variety Pinova, while the measurements showed higher Vitamin C content in case of the variety Golden Delicious.

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Relationships between nutrientsupply, genotype and some physiological properties of winter wheat
141-145

The chlorophyll content (SPAD), leaf area index (LAI) and leaf area duration (LAD) of three winter wheat varieties have been investigated on a chernozem soil. Three winter wheat varieties (GK Öthalom, Pannonikus and Genius) in three different nutrient-supply levels have been involved in our experiment in the crop-year of 2010/2011. The investi...gated physiological properties have been set against to the yield results. Upon the results of this comparison it has been stated that there is a close relationship between the investigated physiological properties and yield averages. The SPAD-values showed a growing tendency parallel to the growth of the nutrient-supply levels. The varieties have the highest SPAD-values at the nutri ent-level of N120+PK. Compared to the yield averages we have stated that varieties that have higher SPAD-values have realized higher yields as well. Higher nutrient dosages have increased in the leaf area. In case of all the three varieties the highest LAI-values were measured at the flowering stage (23. 05.) in the treatment whit N120+PK. Varieties with higher leaf area have produced higher yields as well. Parallel to the increasing nutrient-supply levels the value of leaf area duration increased as well. We measured the highest LAD-values in the treatment of N120+PK nutrient-level. By the LAD-values it can be stated that more durable and larger leaf area has been produced in the flowering and ripening phenophases, due to the higher nutrient-dosages. Analysing the relationships between the results it can be stated that there is a strong positive relationship between fertilizer treatments and SPAD-, LAI- and LAD-values. The genotype showed a strong positive correlation to SPAD-values. Yield averages showed strong positive correlation to SPAD-, LAI- and LAD-values as well.

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Plant growth analysis of wheat populations in a long-term field-experiment
147-151

The experiments were conducted as part of the long-term trial adjusted, in triculture (pea-wheat-corn) and biculture (wheat-corn), at three nutrition levels, with the use of one crop protection technologie (conventional) at the Látókép Research Site of the Centre of Agricultural Scienses, University of Debrecen, on a chernozem soil. The whea...t variety used in the long-term trial was GK Csillag, which was sown at 5,8 million germs/ha.
The effect of pre-crops and nutrient-supply levels on some growth-parameters (LAI, HI, LAD), just as SPAD-values and yield amounts of winter wheat has been investigated in this experiment. We tried to find out the extent of relationship between the different parameters, so we determined the relationships between different nutrient-supply levels, yield amounts, LAI- SPAD- and LAD-values – measured in the crop-year of 2010–2011 in different crop rotation systems – by using correlation analysis. It has been stated both in case of bi- and tri-culture crop-rotation systems that different fertilizer dosages had significantly affected the leaf area index dynamics and its maximal value, and that increasing N+PK fertilization has
significantly increased the duration of leaves, as well. The highest SPAD-values were measured during the flowering and grain filling stages. However, we haven’t revealed significant differences between all fertilizer treatments. In case of the bi-culture crop-rotation system harvest index values showed an increasing tendency parallel to the increasing nutrient-supply levels, while in case of the tri-culture system this tendency was rather decreasing. However, these differences were
not significant. Parallel to the increasing fertilizer dosages yield results were increased in a significant extent. At the same nutrient supply-levels 2088–4615 kg ha-1 higher yields were measured in the tri-culture than in the bi-culture system. The correlation analyses have confirmed that all of the investigated parameters (yield amount, LAI, SPAD, LAD) had almost in all cases close positive correlation to the nutrient-supply level and the yield amount in both crop-rotation systems. These results have confirmed that the leaf area, the leaf duration, the SPAD-values and the fertilization have altogether resulted in the production of maximum grain yields.

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