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Sex expression of flowers in cultivated sweet and sour cherries
50-52.Views:353During the period of 1968-1972 nine sweet cherry varieties were investigated by the author on mazzard seedling rootstock, moreover cv. Germersdorfi óriás and cv. Münchebergi korai sweet cherry cultivars grafted on mahaleb, cv. Korponai and cv. Sukorói cherries were observed on rootstocks of cv. Cigánymeggy sour cherry seedling, too.
Yet, there are other relations between the different parts of sweet cherry flowers too, which perhaps indicate the effect of rootstocks in Cerasus scions. The flower structure shows feminine character on mahaleb, the effect is intermediate on mazzard, however, the sour cherry rootstock strengthens the male character.
Four sour cherry varieties as cv. Cigánymeggy C. 404. cv. Érdi nagygyümölcsű, cv. Meteor korai and cv. Pándy C. 101 were studied on three rootstocks: mazzard, mahaleb and Cigánymeggy C. 215 seedling rootstocks between 1976 and 1980.
The pistil length, stamen number and relative stamen number diverged significantly on different rootstocks. The results revealed a close negative correlation between the pistil length and stamen number, furthermore noticing that, in the self-fertile cv. Meteor korai and cv. Újfehértói fürtös presented increasing of the relative stamen number, so the fertility decreased, while in the case of self-sterile varieties the change was favourable.
Summing up the results, it is obvious, that the fertility of flowers can be modified by rootstocks and the ecological factors cause sex reversions on different combinations.
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Determination of quality in stored pear fruits by chemical analysis and sensorial judgement
27-31.Views:815Aim of this research/project to determine the external and internal preferences of pear using descriptive sensory analysis, consumer preference. The research was performed on local consumers. Destructive measurements included fi rmness by puncture tests, soluble solids content (SSC), titrated acidity (TA). While there is a general positive trend for increasing preference with fi rmness, some consumers will prefer softer apples, and some will dislike the fi rmest pear. Sensorial judgement is able to classify the attractiveness, preference of properties specifi c for pear. Tests were performed by 13 persons on 4 pear varieties (Bosc kobak, Conference, Packham’s Triumph, Dessertnaia) checking 11 sensorial properties on a scale of 1 to 100 points. Relations of measurements and judgements were processed by correlation analysis. For analysis, the objects were furnished in 2011 from different growing sites (Csenger, Mérk and Nagykanizsa), taking from the store (in January) immediately. Among those the best notes were given to Bosc kobak and Conference coming from Mérk and to Packham’s Triumph grown at Nagykanizsa. It was stated that the success of sensorial judgement depends on the state of maturity, which is diffi cult to guarantee to be synchronous among samples of different varieties. Mature fruits are more praised as a sample of Bosc kobak taken from a chain of department store proved to be of balanced composition regarding its sugar/acid ratio (0.12) and the optimal fi rmness (5.75 N/cm2). Results of the correlation analysis suggest that the thickness of the skin is a decisive component of preference (r= 0.857), the typical pear flavour (r= 0.948), the taste as sweetness and acidity (r= 0.930 and r= 0.813). At the same time, no valuable relation could be detected between the data raised in the laboratory and the preference expressed by the sensorial tests, which should signalise that the opinion of consumers does not depend on any individual parameter obtained in the laboratory (sugar- or acid content, fi rmness) but rather on the complexity of several decisive components together (sweetness, acidity, fl avour, skin, etc.). The consumers’ preference cannot be measured objectively without the aid of adequate expertise and a prosperous surrounding, samples of optimal maturity and a thoughtfully edited judging form.
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Studies on the drought responses of apple trees (Malus domestica Borkh.) grafted on different rootstocks
29-36.Views:1390The morphological and chemical changes in the leaves of eight apple varieties grafted on M4, M26 and MM 106 rootstocks were examined during fruit development in non-irrigated plantation. The aim of the experiment was to examine the defensive mechanisms in apple trees under the mild and serious dryness occurring during fruit development. The content of a relative chlorophyll (SPA D value) of the apple leaves on eastern side of the trees was lower than western and southern sides under drought occurring du ring fruit devclop111ent Under sustained drought from first of July to August the SPAD value, the weight of leaves were larger and the stomata density was significantly larger than in the previous periods. There was a close positive significant correlations bet ween SPAD and stoma density both in the group with less sensitive to drought (r=0,8429) and in the large water demanded group (r=0,9209) equally. The rate of increase in SPAD values and the stoma density was slower in the group with drought tolerant, than in the group required good water supply. The varieties being sensitive to water deficite such as Akane. Pink Lady and Red Rome van Well have a l ready responded intensively to short time of drought by the increasing of carbohydrate and antioxidants product ions. At 42 days of sustained drought as soon as the level of carbohydrate in the leaves decreased, the antioxidant s contents of the leaves rised suddenly in the apple varieties with moderate water requirement s as Greensleeves and ldared. Gala apple variety seemed to be able to defend the drought for longer time because there was no change in relatively high level of carbohydrates and antioxidants of their leaves under severe drought during fruit development.
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Spectral properties of strawberry plants
17-22.Views:477Strawberry varieties were studied in 3 different growing areas of different soil- and ecological properties in Hungary. We have measured the optical spectra of the leaves together with their nutrient content and performed sensory examinations on the deep frozen and melted fruit. The 6 varieties were Elsanta, Marianna, Spadeka, Symphony, Camarosa, and Raurica. The 3 sites were Újfehértó, Pölöske, and Kecskemet. Colour of the leaves was characterised by their lightness and dominant wavelength. Under non-optimal soil and ecological conditions the varieties could be distinguished by their colour — the leaves became lighter and more yellow. The fruits did not produce the genetically determined taste if the plant can not grow in optimal circumstances — we found a negative correlation between the leaf colour in flowering time and the fruit flavour. Leaf colour measurements performed in flowering time make it possible to improve fruit quality by changing the nutrient supply between flowering and ripening.
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Historical background and constraints of a grapevine germplasm foundation in Hajdú-Bihar county, Eastern Hungary
11-17.Views:433The historical background of Debrecen linked to viticulture and wine-making stands mainly on the lack of drinkable water, the necessity of drinkable liquid during wartime and epidemics. The special character of the city evolved together with the changing lives of citizens and the increasing trade importance of the city. Period of Turkish occupation gave impetus to the formation of the 11 vine gardens of the settlement. After the devastation of rootmite and peronospora ‘Kadarica’ and ‘Nagy burgundy’ (‘Blaufrankish’), in smaller proportion - on lower sites – ‘Cabernet’ were planted. As white varieties ‘Ezerjó’, ‘Olasz Rizling’, ‘Kövidinka’, ‘white Mustos’, in smaller proportion ‘Szlankamenka’, ‘Erdei’, ‘Szilvaner’, ‘Mézesfehér’, ‘Bakar’, ‘Veltelini’ (red), ‘Fehér burgundi’ (? white burdunder), ‘Rajnai rizling’, ‘Red Tramini’, ‘Furmint’, ‘Muscat Lunel’, ‘Járdovány’ and ‘Juh-fark’ were planted. After the Trianon treaty in 1920, 2/3rd of Hungary was cut away. Érmellék wine region was also cut in two, thus Debrecen broke away from its wine region. Legal regulations after the World War II. (1959) referred back to variety application advised in 1924 for “place suitable for good wine production, not included in any wine region”, like Debrecen listing ’Ezerjó’, ’Mézesfehér’, ’Olaszrizling’, ’Bánáti rizling’, ’Furmint’, ’Hárslevelű’, ’Kövidinka’, Kecskemét virága’, ’Piros szlankamenka’,’Pozsonyi fehér’; ’Kadarka’, ’Oportó’ and ’Kékfrankos’ (Blaufrankish). The political changes of 1990 and Hungary’s admission to the Eurepoean Union almost annihilated the wine production of Debrecen. However little gardens conserved historic varieties which could date back even to many centuries. Through a local magazine a collecting work was announced pointing to gather ancient local (Vitis vinifera conv. pontica) varieties forming a genebank, established on the experimental station of the University of Debrecen. In 2014, about 112 items were collected (accessions). As a 2nd round of the work, with a more detailed and precise work, further 81 items were put into the reservatum. The latter represent single stuck collection, whereas the first ones are to be studied az mixed items. Most notable accession names (ACENAME) of the work are: ‘Fehér gohér’, ‘Veres gohér’, ‘Fekete gohér’, ‘Kék gohér’, ‘Erdei’, ‘Ezerjó’, ‘Kűbeli’, ‘Rizling’, ‘Mézes fehér’, ‘Dinka’, ‘Madling’, ‘Bakator’ and ‘Kadarka’. Simulteneously with the strenghening and morphological description of conserved stucks genetic identification of the items is being elaborated. Database comprising FAO/IPGRI multi-crop passport descriptors and OIV Primary descriptor priority list are to be published on-line in between the development of the platform.
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Interaction impact of drought stress, nutrient-deficient water, and seed-borne pathogen (Alternaria alternata) on germination and vigor of two tomato varietiesination and vigor of two tomato varieties
48-53.Views:316Tomato (Solanum lycopersicum) is considered one of the leading vegetable plants in the world. This study evaluated the germination and vigor capabilities of ‘Marmande’ and ‘Kecskeméti Jubileum’ varieties under different conditions, including drought stress, nutrient-deficient water, and the effect of seed-borne disease caused by Alternaria alternata when prime and non-prime with salicylic acid. The experiment was conducted in the laboratory in 2023 at Agricultural and Food Sciences and Environmental Management Faculty of University of Debrecen. Results indicated that the ‘Kecskeméti Jubileum’ variety exhibited a superior strength to ‘Marmande’ in the seedling’s dry weight, seedling growth rate, and vigor index under nutrient-deficient water. Nevertheless, when applying levels of drought, the ‘Marmande’ variety had a higher viability rate of 62.5% in comparison with another variety, and the germination rate of the two tomato varieties is at 85.5% in 5% concentration but decreased progressively when exposed to a higher drought concentration of 10%. When using 3 ml of salicylic acid during germination stages, the seedling vigor index of ‘Marmande’ shows a greater index at 165 compared to ‘Kecskeméti Jubileum’, just 108 under the infection of Alternaria alternata. The results of the examination of drought stress, and the effect of Alternaria alternata, one cause of seed-borne pathogens, showed that the percentage germination and vigor ability of the ‘Marmande’ variety performed better than ‘Kecskeméti Jubileum’ under the same conditions.
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The investigation of suitability to various purposes of industrial processing in stone fruit varieties and variety candidates
93-101.Views:1059In the laboratory of Conserve-technology in the Research Institute for Fruit Growing, Company of Public Utility, Cegléd, 6 sour cherry, 6 apricot, 5 peach and nectarine, 6 plum and 4 Japanese plum varieties (canned fruit, juice, dried fruit, deep frozen). The products were evaluated by organoleptic methods on a scale of 1-5 steps. The varieties receiving at least 4 points were listed (in brackets also the respective product was indicated): `Kántorjánosi' sour cherry (for all the three purposes), '13' variety candidate (canned and deep frozen), 'T' var. cand., (canned, deep frozen), 'Érdi bőtermő' (dried fruit), 'R' var. cand. (deep frozen); ‘Ceglédi arany', 'Ceglédi bíborkajszi', 'Magyar kajszi"C. 235' (fibrous juice); `Babygold 5', 'Redhaven' peaches, and 'Caldesi 2000' nectarine (canned); 'Stanley' plum (canned), 'Besztercei Bt, 2' (deep frozen).
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Damages caused by winter frosts, their temporal variation and frequencies in the main fruit growing region of Transdanubia and of the East Tisza regions of Hungary
89-97.Views:1113The aim of the study was the study of winter frost damages, especially their changes expressed in temporal frequencies on the main fruit growing regions of the country. In our earlier paper, we introduced the calculation with the term LT50 as the quantitative expression of temperature threshold, when the lethality halves the survival of plant organs, buds or cells causing 50% death rate. The damage is highly dependent on the temperature and on its duration (length of time), but not at least on the frost tolerance of the fruit trees. The incidence and severity of damage is analysed according to the apricot and peach varieties of their different susceptibility or tolerance too. Four fruit growing regions, two of the in Transdanubia and two belonging to the regions east of the Tisza river have been selected to trace the incidence and severity of frost damages. For that purpose, we analysed the history of the past 60 year period, 1951–2010, utilising the database of the network of 16 meteorological stations of the countrywide service. Being aware of the values of LT50 during the rest period and afterward, the compulsory dormancy caused by low temperature, the number of days, the probability of frost damage could be predicted. The role of the orographical profi le, the height above sea level and the exposition of plantations are also decisive. Within the same plantation, 20–30 m difference of level may cause large diversity in temperature and frost damage. Air circulation and regular incidence of winds within the Carpathian basin modify the occurrence and severity of damages. Lowlands near the southern and northern country borders are particularly exposed to winter frosts. Most damages are reported in February, as temperatures below –20 °C especially if the fi rst part of the winter was mild, or in January was a warm period. With the end of the physiological rest period of the trees, the frost-susceptibility increases signifi cantly, and a cold period of –15 °C may cause heavy damage. This study proves that tolerance of varieties infl uence the damages substantially. By planting frost tolerant varieties, winter frost damages could be diminished by 40–50% at the same growing sites. Present results may also offer a tool to estimate the risk of frost damages and express the security of yields at a given site based on the data accumulated in the database over many years.
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Agronomic evaluation of different lettuce (Lactuca sativa L.) varieties under unheated plastic tunnel
50-56.Views:591Among the leafy vegetables, lettuce (Lactuca sativa L.) is the most widely grown species in the world. Wide ranges of physical properties of the products are used to assess the degree of maturity. In this study, different lettuce varieties were grown under an unheated plastic tunnel in springtime. We evaluated the agronomic properties of different lettuce varieties, evaluated the condition of the plants, and determined the bioactive substances. Larger head weight can be achieved by the ‘King of May’ butterhead (259.31 g) and ‘Great Lakes 659’crisphead (220.40 g) genotypes. A very strong correlation (r = 0.995) was observed between the lettuce head index, and leaf index and both had a strong positive correlation (r = 0.828 and 0.760) with NDVI. The highest SPAD values were measured for cos lettuce ‘Romaine lettuce’ (44.01) and iceberg lettuce ‘Great Lakes 659’ (42.71). However, these genotypes showed the highest (9.52%; 8.74%) dry matter content, too. The red leaf variety 'Lollo Rosso' had the highest total polyphenol content (181.53 GAE/100 g FW). Among the evaluated properties, iceberg lettuce showed favorable morphology, plant condition, and good dry matter content. In addition, between the loose-leaf lettuces, the red leaf lollo type was outstanding with bioactive content.
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Antioxidant, polyphenol and sensory analysis of cherry tomato varieties and landraces
75-80.Views:568Among vegetables produced both for raw consumption and processing, tomato is one of the most important one in Europe, by production area and by yield as well. In the past years several study dealt with the investigation of the inner content of tomato, with special regards to antioxidant content. In this paper cherry tomato varieties and landraces from conventional and organic production were compared. Besides basic investigations sensory analysis were designed and Antioxidant Capacity (AOC) and Total Phenol Content (TPC) were measured. The aim of the research was to compare varieties and to study the effect of variety and production methods on antioxidant capacity and sensory profiles.
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Possibilities and limits of use plastic constructions in fruit growing technologies
71-75.Views:855On the Experimental Station Pallag of Debrecen University different combinations of fruit species and rootstocks have been raised under and without plastic foil cover in 2002. The growth and productivity of the grafts was our objective of comparison. Results revealed substantial differences in fruit set and yield depending on species and varieties. Sweet and sour cherry varieties grew much shorter under the plastic cover, whereas apricots, peaches and plums set fruit much more eagerly compared with the trees outside the plastic cover. Outside the plastic cover, the trees were much more developed at the beginning of their fruiting period. In spite of that, the growing processes were more intense under the foil. The differences are allegedly due to the repeated summer pruning necessary under the restricted space of the foil, on the other hand, due to the root concurrence because of the dense planting. Regarding the inner properties of the fruits, soluble solids, sugar and acids were higher outside, whereas macro- and mezzo-elements (P, K, Ca, Mg) were more abundant in fruits grown under the foil cover. Further efforts to explore those relations with other varieties and rootstocks are justified.
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Xanthomonas resistance in Hungarian 4 spice pepper varieties
73-77.Views:299With a view to further enhance the reputation of Hungarian spice pepper it was necessary to improve resistance to the bacterium Xanthomonas campestris pv.vesicatoria, the most dangerous pathogen of pepper varieties. From among the familiar resistance genes in Hungary only the gene Bs-2 could provide sufficient protection against the aggressiveness spectrum of the bacterium species X.c.pv. vesicatoria. The first results of the resistance breeding are the spice pepper varieties Kaldom and Kalorez. In addition to the Bs-2 gene attempts are also being made at building in a gds gene into pepper, a gene creating a general defense system, a different strategy towards Xanthomonas campestris pv. vesicatoria.
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Agroclimatological properties of growing sites assigned to apple and pear production in Hungary
95-97.Views:842Apple and pear growing sites in Hungary are classified into four regions according to the Hydro-thermic Coefficient: dry, moderately dry. moderately humid and humid. Most of the plantations of apple and pear are located in regions considered as moderately dry and moderately humid. Within that category, the two respective species have different preferences, i.e. the ecological features of Hungary give different opportunities for apple and pear growing. Apple is grown almost everywhere in the country, successfully. The selection of cultivar-regions is needed mainly for increasing competitiveness on the market. Main apple growing regions are listed in 3 large groups. For the definition of cultivar-regions, mainly the configurations of soil and precipitation, i.e. conditions of the soil and opportunities of gaining water were decisive. Market factors are also considered. The area assigned to pear is much less than that of apple, in Hungary. Some well known and popular varieties would require high air humidity which cannot be presented in most of Hungary. Therefore, the possibility to establish regions for pear varieties is restricted, we have to create a particular micro-environment. Two groups are potential. The first one comprises sites where the annual precipitation is 700 mm, at least. There, apple and pear production would compete each other. In more dry habitats (less than 700 mm annual precipitation), micro-environments should be found and only drought-resistant, mainly summer-ripe cultivars should be chosen with, preferably, low tendency of sclereid formation. In that case, neither irrigation could help to produce adequate quality in varieties sensitive to low air humidity.
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Molecular identification of old Hungarian apple varieties
37-42.Views:747Altogether 40, mainly old Hungarian apple varieties were screened with six previously described microsatellite markers. A total of 71 polymorphic alleles were detected (average 11.8 alleles/locus) and the heterozygosity of markers averaged very high (0.8). The genetic variability among the genotypes proved to be so remarkable that as few as three markers from the applied six were enough to distinguish between the 40 varieties. This was also confirmed by the cumulative probability of obtaining identical allele patterns for two randomly chosen apple genotypes for all loci, which value was quite low: 2.53 x 10-5. The molecular identification of these genetically very different old apple genotypes could be very useful in future breeding programs.
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Content of mineral- and sulphur compounds in Hungarian and French garlic varieties
61-65.Views:399In our days about 2 million tons of garlic are grown in the world. Garlic was used as medicine already in ancient Egypt. It contains approx 33 sulfur compounds, several enzymes, 17 amino acids and minerals. We determined the content of diallyl sulfone, boron, calcium, copper, iron, potassium, magnesium, natrium, phosphorus and zinc in fi ve garlic varieties (3 French, 2 Hungarian). We searched if there were signiffi cant differences between the varieties.
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Evaluation of elder (Sambucus nigra) varieties and candidates for the canning industry. Results of the composition studies
102-107.Views:277Sambucus nigra is a very common elder species in Europe. Due to its excellent composition, natural dye content and healing power it can be considered as a biological active plant. In Hungary the cultivation of Sambucus nigra started in the nineties and since that time there is a growing demand for this plant. Sambucus nigra is a special fruit due to its sole.-application for processing industry. Partly fruit products are made of it, partly it is used as a natural dye. In both cases the first step is the knowledge and evaluation of the composition of the various elder varieties and candidates. For that reason composition studies of different elder types were performed in several years at the Department of the Canning Technology of the Szent Istvan University.
The most important sample was the Haschberg variety, which is the sole elder type accepted and allowed for propagation by the government. This plant is grown in the Pilot Plant of the Szent István University in Szigetcsép. Among the studied samples there were some varieties cultivated in the Fertőd Research Station. They were the following types: No. 33, 480 and 481. Besides elder collected wild in Szigetcsep and the very promising early-ripened SZ-CS 21-23 variety originated also from Szigetcsép were investigated. One year it was possible to study the Sambu variety, too. Department of the Fruit Culture of the Szent István University helped to obtain the various elder types and candidates. Along our experimental activities, the following components and parameters were studied: the content of pectin, organic acid, carbohydrate, mineral, vitamin C, total acid, aquesoluble dry material, anthocyanin and the pH.
After having obtained a juice, a concentrate from the species has been made and — after freezing — the aquesoluble dry substance and the acid content, as well as the colour parameters were monitored. Studies were performed in two years (1997-98). On the basis of these results classes were established for all compounds, from which an overall ranking was derived.
It was found that in both years the Haschberg variety proved to have the best composition. Therefore regarding to the overall series of order the experiments performed in the second year supported the results obtained in the previous year. Results have shown that Sambucus nigra has excellent composition beside its curative effect. Its mineral content has surpassed that of the other fruits and it had advantageous acid-and carbohydrate composition. The ascorbic acid content of the Haschberg variety is striking. It can be concluded from the experiments that the Haschberg type has the most advantageous composition, therefore it is suitable for making special curative and exclusive products.
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Influence of various mineral supply on sweet corn root development
49-52.Views:292Investigations into root strength have assumed particular importance with the emergence of American rootworm (Diabrotica virgifera-virgifera) as the number one pest, mainly as a result of monocultural production. Three treatments were applied in the trial: zero control treatment, treatment "A" (g/m2): 4, 47 N + 7, 89 P205 + 19, 74 K20 and treatment "B"(g/m2): 8, 94 N + 15, 78 P205 + 19, 74 K20. Measurements were made to find out whether the treatments had produced any significant difference between the root strength of the two varieties (normal sweet Puma F I and super sweet Dessert R75 Fl) at the two dates of measurement (tasseling, milk stage) in the year 2003. The treatments with a high phosphorous ratio resulted in an apparent increase in root strength with both varieties. There was a detectable difference in root strength also between the two points of measurement, suggesting that root development had remained stable. The treatments produced significant increase not only in the root strength but also in the yields of both varieties.
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Variability of the data indicating the fertility of different plum varieties
51-55.Views:1044Self-fertility and fertility at conditions of open pollination in plum varieties is strictly determined by genetic factors. However, rates of fruit set are highly variable according to growing sites as well as to seasons, which may result from a couple of inner and outer conditions, but mainly from the method applied in seizing the facts of fertility in the experiments planned including the number of replications of treatments. During three successive years, 4-16 trees of each of the four plum varieties have been selected and 16-64 branches were tagged either for checking their fertility as pollinated freely or isolated excluding the access of foreign pollen. The data of fruit set have been processed in order to determine the variability of the data, subsequently, the number of replications necessary to make reliable decisions. Both autogamy and open pollination displayed multiple differences between branches and trees studied.
A number of 20 branches are needed yearly for each variety, the branches should be distributed on 5 trees at least for checking the autogamy, whereas on 10 trees for the results of open pollination. Each variety and treatment should be represented in three seasons, at least because of the different weather conditions.
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Chemical analysis of the pollen of plum varieties
25-26.Views:1058The chemical composition, amino acids and carbohydrates, of the pollen has been analysed in varieties grown in Hungary during the years 1993 at Kecskemet and 1995 at Cegléd and Pomáz. The distinction of 17 amino acids and 7 carbohydrates has been achieved. The aim of the study was to find criteria for the distinction of varieties.
The total content of amino acids varied between 15.53 and 20.54 %. The highest ratios represented the asparagine and the glutamine acids. The relative content in other amino acids was between 2 and 7 % of the total. The less frequent were cysteine and valine.
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Carotenoid composition and content in products of sea buckthorn and peach as determined by high-performance liquid chromatography
69-74.Views:1112A study was conducted to analyse the carotenoids by high-performance liquid chromatography (HPLC) using reversed-phase cross linked end-capping and to study the pigment content and composition in sea buckthorn products as well as in freshly harvested fruits from peach thees grown under organic and integrated farming conditions. It was found that carotenoids in fruits of both crops occur mainly esterified with fatty acids in form of mono- and di-esters. The major carotenoids were esters of zeaxanthin, lutein, β-cryptoxanthin and β-carotene. The major carotenoids showed different response to processing of sea buckthorn being highly sensitive to thermal treatments such as blanching, cooking and drying. Significant differences were found between peach varieties in their carotenoid content, whereas the impact of organic farming on carotenoids formation was found to be variety-dependent. Two of the three varieties examined in this work, when cultivated under organic farming conditions contained lower carotenoid level as compared to that found in the fruits of the same varieties but produced in integrated farms.
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Results in the determination of some Hosta varieties by the method of isoelectric focusing
90-92.Views:363Mass propagation of 5 newly introduced Hosta varieties was carried out by the method of micropropagation. Our aim was to determine exact variety specificity after the micropropagation period in the pattern of peroxidase isoenzymes by isoelectric focusing in pH 3-9 range and to determine that phenological phase of mother plant in which the isoenzyme pattern of mother plant can safely be comparable to the isoenzyme pattern of micropropagated descendants. The isoenzyme patterns of descendants were similar to the mother plants of the same hybrid lines. The older leaves seemed to be not so suitable for examination than newly developed ones despite of the higher activity of peroxidase enzymes. There were big differences in isoenzyme patterns of leaves in different phenological phases. With this quick and easy method Hosta varieties could be selected already in the very early stage of micropropagation.
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Influence of abiotic factors on the green mass and seed productivity of the raw material of Levisticum оfficinalе c. Koch in the conditions of Transcarpathia
46-51.Views:479For the wide implementation of Levisticum officinalе C. Koch in Transcarpathia into production it is necessary to widen its sort composition, as it is rather poor in Ukraine. Solving this problem is possible by enriching the source material with the samples of different ecological and/or geographical origin, by studying the influence of abiotic factors on the formation of green mass and seed productivity, separating the specimens best adapted to the conditions of growing, involving them into the selection process and creating new, highly productive sorts on this basis. The scientists of the Transcarpathian State Agricultural Experimental Station (TSAES) conducted researches (during 2011-2016) on the adaptive potential of the source material of the type of Levisticum officinalе C. Koch, which counts seven samples (Ukraine – 5 samples, Poland – 1, and Romania – 1), the selection work on creating new varieties for the given region is being done. The investigations were made on soddy podzolic gleyed low-humus soils with low content of nutrients. The results were obtained on the basis of generally accepted methods of investigation, i.e.: laboratory, field, visual, measuring-weighing, biochemical, mathematically statistic, and comparative-calculating. In the conditions of Transcarpathia the spicy greens of the high quality L. officinalе C. Koch are being gathered on days 25-30, the output of the essential oil makes up from 0.72 tо 1.89% on c.d.s., depending on the climate conditions of growing. The plants pass through all the stages of development, the bush is 55.4 to 92.7 сm in height, the plants form a big amount of rosette-shaped stems (11-34 pieces), the productivity of spicy greens is between 9.9 and 42.1 t hа1. The scientists of TSAES created the following sorts – Mriya (А. с. 06131 Ukraine, first registered in the State register of plant varieties of Ukraine) and Coral (Pat. on the variety № 140830). Economic effect from growing of the Coral variety in the conditions of Transcarpathia is 10230 UAH/ha and the level of profitability is 28.4%. The geographic position and agroclimatic conditions of Transcarpathia, as well as the unpretentiousness of L. officinalе C. Koch. tо the conditions of growing contribute to the enrichment of the local flora with useful plants; and the rich chemical composition contributes to the spread of this culture in the given region.
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In vitro multiplication and hardening of grapevine plants in aeriated media
15-18.Views:378In vitro cultures have widely been used in horticulture for rapid multiplication of new varieties and clones as well as to produce pathogen-free stock material. To improve efficient hardening and transfer in vitro grown grapevine plants were multiplied by cutting them into single-node internodes with the whole leaf. Microcuttings including the shoot tips were rooted in granulated perlite moisted with tapwater under sterile conditions. After 2-3 weeks the rooted microcuttings were supplied by nutrients and hardened by gradual opening and finally by complete removal of the lids of jars or plastic boxes used for growth. Using this method microcuttings of Vitis vinifera cvs. „Chardonnay", „Cabernet franc", „Riesling" and „Sauvignon blanc" and the rootstock varieties Vitis riparia x Vitis cinerea cv. „Barrier" and Vitis berlandieri x Vitis rupestris cv. „Richter 110" formed new roots and shoots and 100% of the tested plants survived the acclimatization procedure. Similar results were obtained when perlite was replaced with rockwool-, or pit-pot blocks. This method may highly increase the efficiency of producing pathogen-free propagating material and new transgenic lines.
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Appreciation of ethrel on ripening dynamic and on the content of ingredients in processing tomato (Lycopersicon lycopersicum (L.) Karsten) varieties
33-35.Views:336Tomato (Lycopersicon lycopersicum (L) Karsten) is an important crop cultivated in Hungary. Ethrel has been used to advance maturity and promote uniform ripening of processing tomato since 25-30 years in Hungary. The aims of the present study were 1) to evaluate the effects of two different ethrel concentrations on ripening rate, 2) to investigate lycopene content of different maturity stages, 3) to test the effect of ethrel on lycopene content. It is important to note that the experimental year (in July and August) was very rainy and cool. Ethrel was applied at two rates: 1500 and 3000 ppm. The results clearly indicate that Ethrel can be a useful and effective tool of maturity-enhancement, under present circumstances. Ripening concentration increased significantly by Ethrel. In spite of this, Ethrel treatments did not affect lycopene content of examined varieties significantly. The quality of tomato products are characterised by their lycopene content. Colour is highly important quality factor of food products. The range in lycopene contents from all samples evaluated was 48.7 to 113.0 mg kg-1 fresh weight. Also correlations between lycopene content and colour (a*/b*, and chroma) were investigated also.
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The effect of bud density on the composition of colour in red wines
63-68.Views:369Authors investigated the effect of fruit load on the composition of colour substances and of reservatrol in red wines of some grape varieties grown in Hungary. The content of both, anthocyanin compounds and reservatrol showed practically the same responses to fruit load and the same profile in all the varieties studied. Determination of these compounds solely is not sufficient to identify the respective grape variety.
