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Apple allergens as affected by cultivation technolog and variental factors
91-93.Views:862Our objective was to study the effect of organic farming and area of cultivation on the allergen content of apple varieties by quantification of the major allergens (Mal d 1 and Mal d 2) using allergen-specific ELISA. The obtained results showed that except Idared IX. 29. most of examined varieties, when cultivated under organic farming conditions, contained lower level of Mal d1 allergen than those produced with integrated farming. The different varieties were found to vary substantially in theirMal d 1 allergen content. Furthermore, with some varieties the area of cultivation affected to some extent the content of Mal d 1 allergen.
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The effects of ACS (1-aminocyclopropane-l-carboxylate synthase) gene down regulation on ethylene production and fruit softening in transgenic apple
65-70.Views:224A detailed examination of the production of ethylene and other ripening parameters during storage period has been undertaken in transgenic apple fruits, where the ethylene biosynthesis was inhibited by antisense ACS (l-aminocyclopropane-l-carboxylate synthase) gene. Data indicate down regulation of ethylene production, softening and spoilage in some transgenic lines. In some cases ethylene production was inhibited for over 90 percent, considerable reduction of softening and spoilage was observed probably due to the reduced activity of cell wall degradable enzymes. ACS activity was also monitored during ripening. The fruits of the best transgenic lines could be stored for minimum 4-5 months longer under 5 °C cold room storage conditions and one month longer at normal room temperature. This molecular approach can provide an alternative way to replace the commonly used and costly atmospheric storage of fruits.
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Fruit coloration of apple cultivars
11-14.Views:567One of the most important parameter of apple cultivars is the scale and the intensity of the coloration of fruits. Nowadays among the bicolour cultivars those are the most saleable which have the highest and darkest fruit coloration. In our three years old study cultivars born with bud mutation and cross breading were examined, which have superior coloration peculiarities than their parental cultivars. According to our results among the observed cultivars the cvs. ’Gala Decarli-Fendeca’, ’Galaval’, ’Wilton’s Red Jonaprince’, ’Early Red One’ and ’Red Cap Valtod’ reached favourable high fruit surface colour (80-100%) regardless the vintage effect. Concerning the colour intensity, that is the darkness of the red colour most of the cultivars (‘Gala Venus Fengal’, ‘Gala Fendeca-Fendeca’, ‘Galaval’, ‘Jeromine’, ‘Red Topaz’, ‘Early Red One’, ‘Crimson Crisp’, ‘Wilton’s Red Jonaprince’) showed excellent values (4,5-5,0). Based on the fruit surface colour and the fruit colour intensity a new index number, the fruit coloration index was created by us, which describes the coloration features in a complex way.
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Effect of pruning intensity on performance of apple cultivars in environmentally friendly production systems
65-67.Views:888The two worldwide accepted environmentally freindly production systems are integrated and organic. The two systems shows considerably differences in several practical production technology elements. These effects come forward in generative processes (e.g. flowering, fruit setting) and vegetative features (e.g. growth). The aim of our study was to study the effect of pruning intensity on growth features of cultivars in integrated and organic apple production. In early spring of 2009, we determined two pruning intensities (strong and week) on six scab resistant and tolerant cultivars. Strong pruning resulted in stronger shoot growth for all cultivars compared to weak pruning. It is obvious that pruning intensity had more effect on vegetative performance than conditional differences originates from integrated and organic production.
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A short introduction of new apple varieties obtained
135-137.Views:462A short introduction of new apple varieties obtained
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Comparative analysis of the production risk of major fruit species
119-122.Views:269Hungary possesses excellent agri-ecological potentials even in an international comparison. Despite their decreasing economic weight, agriculture and food production are of great significance, since the supply of the population with high quality and inexpensive food is a fundamental social demand and one of the essentials of the quality of life. The development of vegetable and food production is of great importance both from the aspect of healthy nutrition and comparative advantages (cheap workforce, professional expertise, traditions of production, climate favourable for quality). Within the Hungarian fruit production apple and sour cherry are essential branches in virtue of their significance. It is widely known, that – though to a different extent in the case of certain fruit varieties – satisfaction of the need for appropriate habitat is one of the important conditions of a decent quality and secure production. In my research, I analyse the territorial distribution, production standard and production risk of the Hungarian apple and sour cherry branches. I did not have the opportunity – because of the imperfections of the database – to compare long time series, therefore I examine the random effects affecting production by using the data of 3 years (they have the same content). I ranked and assessed each habitat and the production risks of certain fruit species compared to each other on the basis of indexes of relative deviation.
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Effects of aromatic cytokinins on structural characteristics of leaves and their post- effects on subsequent shoot regeneration from in vitro apple leaves of 'Royal Gala'
41-46.Views:358The effects of different aromatic cytokinins applied in different concentrations and combinations were investigated on the histology of in vitro apple leaves and their post-effects on subsequent shoot regeneration from these leaves were studied. Great differences in the anatomical structure of leaves could be detected originating from media containing different types and concentrations of aromatic cytokinins. The number of regenerated shoots per explant and the organogenetic index were used for the evaluation of the post-effect of aromatic cytokinins on shoot regeneration. The histological structure of leaves used for regeneration and their regeneration response showed a good correlation. When the pre-treatment caused a juvenile-like or less-differentiated structure, the number of regenerated shoots per explant increased and often vitrification also decreased and consequently the organogenetic index also increased. A strong interaction between cytokinin-content (type and concentration) of the pre-treatment medium and that of the regeneration medium could also be detected.
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Fire blight (Erwinia amylovora) resistance in apple varieties associated with molecular markers
53-57.Views:415The invasive bacterial disease fire blight, caused by Erwinia amylovora has the potential to destroy fruit tree orchards all over Europe. Effective plant protection methods are lacking in many countries, highlighting the increasing importance placed on identification of germplasm with heritable disease resistance. Recent l y. a promising QTL (quantitative trait locus) was identified on linkage group 7 in the apple cultivar 'Fiesta· which is derived from ·cox's Orange Pippin' . I n the present study, 144 Swedish and foreign apple cultivars were analysed with the SCAR markers AE I 0-375 and GE-8019. which flank-. this QTL. Twenty-nine of the analysed cultivars had both markers. 78 had either AE I 0-375 or GE-8019, and 37 cultivars did not carry an) of the two markers. Seventeen cultivars. 7 with both markers and I 0 not having either of the two markers, were then inocoluted with the bacterium in a 4uaran1i ne greenhouse test. Cultivars carrying both DNA markers were significantly less susceptible than cultivars lacking the markers, P<0.001. Cultivars that were most resistant had both markers and had 'Cox· in their pedigree. Unrelated cultivars with the markers may still lack the QTL.
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Effects of weather characteristics on blooming dates in an apple gene bank plantation between 1984 and 2001
37-44.Views:724The aims of this paper was to investigate the flowering characteristic of apples and their relationship to meteorological parameters. The trees observed are grown at Ujfehert6, Eastern Hungary in the plantation of an assortment (gene bank) with 586 apple varieties. Each of the varieties were observed as for their dates of subsequent phenophases, the beginning of bloom, main bloom and the end of bloom over a period between 1984 and 2001. During this period the meteorological data-base keeps the following variables: daily means of temperature (°C), daily maximum temperature (°C), daily minimum temperature (°C), daily precipitation sums (mm), daily sums of sunny hours, daily means of the differences between the day-time and night-time temperatures (°C), average differences between temperatures of successive daily means (°C). Between the 90th and 147th day of the year over the 18 years of observation. The early blooming varieties start blooming at 10-21 April. The varieties of intermediate bloom start at the interval 20 April to 3 May, whereas the late blooming group start at 2-10 May. Among the meteorological variables of the former autumnal and hibernal periods, the hibernal maxima were the most active factor influencing the start of bloom in the subsequent spring.
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Relationship between several meteorological factors and phenological features of apple cultivars
13-19.Views:723The trees observed are grown at Ofeherto, Eastern Hungary in the plantation of an assortment (gene bank) with 586 apple cultivars. Each of the cultivars were observed as for their dates of subsequent phenophases, the beginning of bloom, main bloom and the end of bloom over a period between 1984 and 2001. during this period the meteorological data-base keeps the following variables: daily means of temperature (°C), daily maximum temperature (°C), daily minimum temperature (°C), daily precipitation sums (mm), daily sums of sunny hours, daily means of the differences between the day-time and night-time temperatures (°C), average differences between temperatures of successive daily means (°C). Between the 90th and 147th day of the year over the 18 years of observation. The early blooming cultivars start blooming at 10-21April. The cultivars of intermediate bloom start at the interval 20 April to 3 May, whereas the late blooming group start at 2-10 May. Among the meteorological variables of the former autumnal and hibernal periods, the hibernal maxima were the most active factor influencing the start of bloom in the subsequent spring.
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Pruning and incidences of diseases and pests in environmentally oriented apple growing systems: some aspects
24-29.Views:582In Hungary, fruit growers are increasingly interested in environmentally oriented growing methods, such as organic and integrated systems. The main factor in creating such systems is the establishment of a system of cultivar-pruning-plant protection based on production technology. Therefore, our aim was to examine the susceptibility of apple cultivars to diseases and pests and the effect of pruning technique on diseases and pests in organic and integrated growing systems.
Two pathogens (Venturia inaequalis, Podosphaera leucotricha) and two pests (Leueoptera malifoliella, Pannonychus ulmi) were observed in organic and integrated systems under "strong" and "weak" pruning techniques. Our results showed that, compared to integrated production, the pathogens and pests infected the trees more in the organic system. The pruning technique affected mainly the cultivars susceptible to diseases and pests. In general, the "weak" pruning technique correlated with a higher level of damage caused by diseases and pests than the "strong" pruning technique, especially in the organic growing system. The reason that techniques should be carefully chosen is that shoots grow faster and more vigorously after employing the "strong" pruning technique. This supports a better preservation of the trees, as a result of reduced susceptibility of plant tissues to diseases and pests.
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The inheritance and durability of scab resistance in apple progenies
39-46.Views:298In order to select the appropriate parent cultivars and maintain the durability of resistance, it is important to clarify the mechanisms of inheritance of scab resistance depending on the parents. It has been known that the progeny segregation ratios based on scab-resistance do not depend only on the genotype of the resistance locus but also on the genetical makeup of the donor and recipient parents as well as on the susceptible parent.
The aim of this study has been to demonstrate what factors in the Vf, Vr and VA scab-resistant cultivars — combined with susceptible and resistant parents — affect the inheritance and durability of resistance in seedlings in their first 4 year's growing four years' growth. After inoculating apple seedlings sown in 2001 with the suspension of Venturia inaequalis (Cke.) Wint. in the greenhouse, we studied the segregation ratios of the progenies into reaction classes. Seedlings showing resistance in the greenhouse were also evaluated for scab-resistance after they had been moved to the field and had naturally been infected with the pathogen in 2002 and 2004.
The majority of our results obtained in the greenhouse test, similarly to earlier experiences, have not justified monogenic inheritance at the phenotypic level. The effect of susceptible parent cultivars on the segregation ratio of progenies have become apparent again. The high infection rate of seedlings in the field trials, which had previously exhibited varying degrees of resistance in the greenhouse test, has raised concern. Our data has raised further doubts, concerning the durability of Vf resistance in Hungary. It is assumed that the composition of natural field populations of Venturia inaequalis in Szigetcsép has changed. The complexity of Vf resistance has been confirmed. The high infection rate in the progenies derived from Vf resistant cultivars draws the attention to the importance of utilizing additional sources of resistance.
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Contributions to the resumption of growth in ecodormant buds of apple
35-40.Views:237The resumption of development in ecodormant buds in terms of establishing a functional vascular connection between the inflorescence primordia and spur tissues in apple trees was investigated. Differentiation of the xylem elements could be observed first in the pedicel of the flower primordium, in the middle of January. Much later (at the beginning of April) there were mature xylem vessels in the wall of the receptacle and, merely a procambial strand for the ovule primordium which was at this time an undifferentiated protrusion of meristematic cells, only. As for phenological development of buds incubated at a temperature of 20 °C, it was the slowest in buds sampled in January, faster in buds sampled in the middle of February and, buds from the middle of March responded very quickly. The function of temperatures needed both for xylem differentiation and for the flower primordium to achieve maturity is pointed out. The nature of frost damage in vessel elements, as well the relationship between chilling requirement and growth features of apple cultivars will be discussed.
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Genetic diversity in a collection of apple (Malus x domestica Borkh.) cultivars as revealed by RAPD markers
25-35.Views:291A collection of 151 apple cultivars was investigated with 7 RAPD primers generating 71 informative bands, to evaluate genetic variability and relatedness. All cultivars presumably derived through genetic recombination were distinguished whereas identical DNA profiles indicated that some cultivars had arisen as sports. A cluster analysis and a PCO did not reveal any distinct geographic patterns, but there was a weak tendency for Swedish and foreign cultivars to differentiate.. Many cultivars however clustered together with either one of their parents or with siblings. Overall genetic diversity among the 151 cultivars was estimated with Nei's diversity index (H), 0.269, and with Shannon's index (H'), 0.594. The cultivars were also analysed in six groups, according to time of origination and country of origin, with an average H = 0.262 and H' = 0.546. No major differences in genetic diversity were observed over time or space, although the group with recent, foreign cultivars had the lowest diversity (FL = 0.235, H' = 0.493). Comparison between the entire material and a subset with 94 mandate cultivars chosen for preservation in Sweden, showed similar genetic diversity: HFN.rj1E = 0.268, frE,NTIRE, = 0.593 and HMANDATE = 0.263, WMANDATE = 0.575. No major differences in band frequencies were observed between these two sets, but 5 RAPD bands were missing in the set with mandate cultivars.
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Identification of the apple firmness: two case studies
32-35.Views:206Firmness tests were performed with peeled and entire fruits of Elstar and Jonagold apple cultivars for identification and comparison. The normal distribution of the tested population was acceptable (level: 95%). The green and the red sides did not show differences within the cultivar but they were different in firmness. There was not significant difference between the flesh tissue firmness values, however the firmness of the entire (not peeled) fruits was different. This result was caused by the effect of the peel. The variability of the firmness with Jonagold was caused by the peel, but such a result was not found with Elstar. The test of the peel effect would be interesting with different cultivars and a sequence according to the firmness can be estimated.
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Preliminary study on micro area based spatial distribution of powdery mildew in an organic apple orchard
35-37.Views:364In this study, the objective was to report a preliminary study on micro area based spatial distribution of powdery mildew in an organic apple orchard. Results showed that number of symptomatic plant part ranged between 11 and 20 on shoot and between 9 and 24 on fruit. Number of asymptomatic plant part ranged between 85 and 109 on shoot and between 133 and 206 on fruit. Disease incidence ranged between 13.8 and 17.6% on shoot and between 9.1 and 11.3% on fruit. Disease aggregation index ranged between 0.098 and 0.228 on shoot and between 0.043 and 0.108 on fruit. One of the four trees showed significant within canopy aggregation of disease for shoot powdery mildew symptoms in both years. For leaf powdery mildew, all tree exhibited random patterns in both years.
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The effect of the intensity and method of pruning on the growth and yield of the apple variety ’Idared’ under conditions of ecological and integrated growing systems
35-39.Views:894Summary: On the Experimental Farm of the Debrecen University at Pallag, a factorial experiment has been started at springtime of 2008 on an orchard of 12 year old apple trees ’Idared’ grafts on M 26 understocks. The first factor was the growing system (integrated versus ecological), the second was the intensity of pruning (strong versus moderate thinning of the crown), whereas the third was the method of pruning (shortening versus thinning of individual shoots). Measurements have been concentrated on shoot growth, flower initiation, fruit set and accumulated yield of two successive seasons. The following conclusions are made.
– In the ecological growing system, strong thinning proved to be beneficial for growth as well as for fruit bearing. – Strong thinning combined with shortening of shoots diminished yield in the first year, whereas
moderate thinning combined with shortening of shoots caused favourable growth and fruit bearing.
– In the ecological growing system, the moderate shoot growth was associated with better flower initiation, which increases the chances of alternate yielding.
– According to our results, the flower initiation of the ‘Idared’ was stimulated univocally by the thinning of shoots regardless of the intensity of pruning.
The right choice of the intensity and method of pruning is a decisive moment of a successful yield in the ecological apple growing system -
Prediction infection risk on the basis of weather-related factors and Erwinia amylovora colonization in apple and pear flowers
39-54.Views:254Current infection risk prediction models utilize environmental parameters and field records, but do not take into account the estimated inoculum potential within the orchard. The object of this study was to survey the accuracy of three simple prediction methods under Hungarian climatic conditions, which could easily be used by the farmers. We also tested whether the accuracy of infection risk predictions can be improved by taking into consideration the incidence and/or rate of flower colonization by Erwinia amylovora.
After preliminary investigations in 1999-2001, data concerning the weather-related infection risk were recorded in 5 apple and 1 pear orchards in 2002, and in 12 apple and I pear orchards in 2003. The weather data were processed by the easy-to-use risk assessment models of the mean temperature prediction line (MTL), Smith's Cougarblight 98C and Billing's integrated system (BIS), and by the MaryblytTM 4.3 computer-assisted model for reference. The population size of E. amylovora in the flower samples was estimated within an order of magnitude by PCR.
For all years and orchards tested, Maryblyt indicated 35 days on which there was an acute infection risk. The same days were indicated by all 3 methods in 23 cases (66%), 8 days were indicated by 2 methods (23%) and 4 days were indicated by 1 method only. A similarly good correlation was found for prediction of the date of the first massive infection risk: in 2003, for instance, there was a perfectly consistent prediction by all 4 models in 9 of the 13 participating orchards. A coincidental forecast was provided by 3 of the 4 models in the other 4 orchards.
The results indicate that any of the risk assessment models could provide an increased accuracy of the actual infection risk prediction if combined with an estimation of the incidence of Erwinia amylovora colonization in the open flowers. We found no convincing differences in the size of the epiphytic population in flowers of cultivars possessing high or low susceptibility to Erwinia amylovora.
We conclude that the easy-to-use methods tested could be used by the fanners to recognize weather-related risks, especially when coupled with an estimation of the proportion of the pathogen-infested flowers. This local prediction would provide rapid information (faster than the regional forecast systems) specifically for a given orchard.
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Yield and fruit quality response to foliar application of biostimulants in an apple orchard
17-23.Views:424Nutrient supply plays an important role in fruit production technology. Among the methods of nutrient management, the application of foliar fertilization has become an essential tool to harmonize the vegetative and generative performance of fruit trees. This study was conducted to determine the effect of four foliar fertilizers (Bistep, brown juice, Trichoderma harzianum, Clonostachys rosea) on yield and fruit quality in an apple orchard with the cultivar ‘Pinova’, at the University of Debrecen in Hungary. Trees were trained to a slender spindle canopy with a spacing of 4 × 1 m. Treatments were applied five times during different phenological phases in 2021 and 2022. Based on our results, biostimulants had a positive effect on fruit yield and fruit quality, but extreme weather events also influenced the data. In 2021, the effect of the treatments on yield data could not be observed (10.1–13.5 kg/tree; 0.16–0.24 kg/cm²). However, in 2022, plants sprayed with Bistep, T. harzianum, and C. rosea reached 28.7–31.2 kg/tree (0.33–0.46 kg/cm²), while control trees produced only 19.8 kg/tree (0.20 kg/cm²). Fruit size development improved with an increase of 2–6 mm over the two years. Among the treatments, Bistep was able to enhance fruit surface coloration to a greater extent in 2021, as red skin color reached 44% for this foliar fertilizer, while control trees presented only 27%.
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Flower production of apple varieties grown by different environmental technologies
47-49.Views:297The flower production of four apple varieties have been observed grown in integrated and in ecological growing technologies during the spring of 2010. The trees produced in the last year (2009) nearly similar yields, so the effect of the technologies may causen differences in the production of flowers. According to our results, the ecological technology produced higher flower densities in the majority of variables, which was in relation with the vigor, branching and thickening of the trunks. The varieties observed displayed substantial differences in the appearance of fruiting structures of different age on the branches. This information may prove to be essential for the decisions to be made in timing and severity at the pruning operations.
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Spore dispersal, diurnal pattern and viability of Monilinia spp. conidia and the relationship with weather components in an organic apple orchard
17-19.Views:497In a two-year Hungarian study, spore dispersal diurnal periodicity and viability of Monilinia spp. and their relation to weather components were determined in an organic apple orchard. Conidia of Monilinia spp. were first trapped in late May in both years. Low number of conidia were trapped until end-June. Thereafter, number of conidia continuously increased until harvest. Conidia in a 24-h period showed diurnal periodicity pattern, with th highest concentration in the afternoon hours. Spore viability with FDA staining showed that viability of
conidia ranged from 45 to 70% with showing lower viability in the dry than in the wet days in both years. Temperature and relative humidity correlated positively with mean hourly conidia numbers in both years. Mean hourly rainfall was negatively but poorly correlated with conidiacatches in both years. Results were compared and discussed with previous observations. -
Integration of terrestrial laser scanning and spectral canopy scanner in horticulture applications
15-18.Views:730One of the most difficult challenge in the everyday practice to describe the canopy growing of fruit trees in an orchard. The photosynthetic activity is the basic of the primer production of plants. The measurement of leaf area and determination of the photosynthetic activity could be occurred with some elaborated methods between experimental conditions. In this article we present such an integrated methodology, which is ideal to determine the geometric and spectral characteristic of fruit trees between field conditions.We have carried out laser scanning technology to investigate the geometric-topological characteristics and parallel the active infra-red sensor to collect spectral data about an apple orchard. The surveys were worked out in an intensive apple orchard with drip irrigation system, protected by hail net in Study and Regional Research Farm of the University of Debrecen near Pallag. This study shows the filtering and interpretation methods of created data. The produced high accuracy data can be directly used in the precision horticulture. It could serve as a guiding data to implementation a future “virtual horticulture”. Higher spatial and temporal resolution could help for a better recognition of water balance of orchards.
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Test of the utility of apple retrotransposon insertion patterns for molecular identification of 'Jonathan' somatic mutants
7-10.Views:482Up until today, apple sport mutants proved to be indistinguishable from each other and their progenitors at the molecular level using random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) marker techniques. This is not surprising, since the genomes of these somatic mutants differ only in one or a few small regions that affect economically important characteristics, such as improved fruit colour, size, or flavour. In most cases, these genome differences are probably caused by retrotransposons which are able to convert their RNA transcripts to DNA with reverse transcriptase enzyme prior to reinsertion, but unable to leave the genome and infect other cells. Retrotransposon insertions can alter the expression of other genes and/or the structure of encoded proteins. The sequence-specific amplified polymorphism (S-SAP) technique is capable of revealing the genetic distribution of retrotransposable elements over the whole genome. The present study used this approach to try to characterize and distinguish 'Jonathan' somatic mutants via fingerprinting, which is an unsolved problem.
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Dynamics of the uptake of nutrient elements from the medium of in vitro cultured apple rootstocks
50-53.Views:271Cation uptake of J-TE-F apple rootstocks propagated in vitro was studied by the analysis of culture medium. Test-plants were grown on liquid medium under different light regimes. Samples for tests were taken twice a week.
Media without plants served as controls. The analysis of those showed, that only the uptakeable iron-content changed depending upon light treatment. The concentration of all other cations was considered unaltered.
As a result of analysis, it could be established, that elements present in the media were taken up in different rates by plantlets: Cu, P and Zn were utilized totally, but only 50% of K and 20 to 40% of Ca and Mg were taken up under the light treatments applied.
The dynamics of the uptake process was also observed. It was registered that they differed in the case of some cations. So Ba was utilized at the beginning of subculture, others for example B in the later phases. Some elements disappeared unevenly so K, P but the whole quantity is taken up during subculture.
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Preliminary study on micro area based spatial distribution of Monilinia fructigena in an organic apple orchard
19-21.Views:415In this study, we aimed to report a preliminary study on micro area based spatial distribution of Monilinia fructigena in an organic apple orchard. Results showed that number of symptomatic fruit ranged between 22 and 42 in 2013 and between 25 and 35 in 2014. Number of asymptomatic fruit ranged between 111 and 187 in 2013 and between 119 and 167 in 2014. Disease incidence of fruit ranged between 19.7 and 23.2% in 2013 and between 19.1 and 26.5% in 2014. Disease aggregation index ranged between 0.111 and 0.335 in 2013 and between 123 and 401 in 2014. Three of the four trees showed significant within canopy aggregation of disease for fruit brown rot symptoms in both years. However, the remaining one tree exhibited random patterns during both years. Disease aggregation indicated a disease spread by fruit-to-fruit contact and/or an aggregated
pattern of insect damage.
