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First results of varios sour cherry cultivar on Oblacsinska as interstocks and Prunus mahaleb rootstocks
87-90.Views:13855At the experiment orchard of the Fruit Research and Extension Institute, Újfehértó, Hungary in spring 2000 a trial was designed included: Erdi bőtermő, Debreceni bőtermő, and Csengődi sour cherry (Prunus avium L) cultivars which were budded at 1999 on Oblacsinska sour cherry (as a dwarf inter stock), which had been budded on Prunus mahaleb seedlings at 1998 with the aim to investigate the dwarfing effects of Oblacsinska sour cherry cultivars on scion cultivars. Trees of each cultivar directly on Prunus mahaleb seedling, severd as controls. Use of Oblacsinska as an interstock reduced the rootstock trunk diameter. Trees of Debreceni bőtermő, and Cseng6di sour cherry cultivars with Oblacsinska interstock were smaller than similar trees grafted directly onto Prunus mahaleb as a rootstock. Trees of Erdi bőtermő grafted on Oblacsinska interstock which buded on Prunus mahaleb were the smallest. Growth of the trees was reduced also by using of the Oblacsinszka as interstock for other examined cultivars.
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Disease progress of apple scab caused by Venturia inaequalis in environmentally friendly growing systems
56-62.Views:520Progression of apple scab epidemic in six apple cultivars, including two current and susceptible (Gala Must, Elstar), two old (Egri Piros, Darusóvári), and two resistant cultivars (Relinda, Releika), were described and analysed in a two-year-study, in two environmental-friendly growing systems (organic and integrated). Curves of disease progress, linear regression analysis of transformed disease incidence data and Area Under Disease Progress Curves (AUDPC) were used to characterise the epidemic processes of the selected cultivars. Cumulative disease progress curves showed continuous but asymmetrical scab development on the moderate or highly susceptible cultivars Gala Must, Elstar and Egri Piros, and on the tolerant or resistant cultivar Darusóvári and Relinda, in both systems. The cultivar Releika showed no symptoms either on fruit or leaf. In linear regression analysis, the best linearisation was given by logistic transformation. Adequate parameters leaf disease incidence rate, of obtained from a regression equation, were higher in the organic system than in the integrated system. Values of AUDPC showed great differences in leaf disease incidences among cultivars and between growing systems. AUDPC gave more differences for comparison of progresses of disease epidemic than growth rate of disease in different systems of disease control. Moreover, the obtained results were compared with similar studies on different pathosystems, and biological interpretations of the analyses are discussed below.
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Differing effects of grass and polythene mulches on sweet potato productivity in derived savannah agro-ecology
24-30.Views:422Sweet potato as a food crop that is grown for both its tuberous roots and vegetative parts, the latter of which also serves as animal feed. Its production in the savanna regions can be limited by high evaporative demand, especially where soils with low water retentivity are involved. This work studied the effects of various locally available polythene as mulch materials for sweet potato growth in derived savanna agro-ecology. An experiment was thus conducted at Nsukka, a derived savannah in southeastern Nigeria, with orange fleshed variety of sweet potato. Treatments were polythene mulches of contrasting colours (black, white, red and blue polyethylene), including also organic mulch (dry grasses) as reference and a no-mulch control. The experiment was executed in two successive cropping seasons, and the data averaged and analysed. Agronomic and weed incidence data collection was done fortnightly from 2nd to 10th week of planting. The results showed that the polythene mulches (especially the black one) were better in the control of weeds. Furthermore, the sweet potato yields were affected significantly (p < 0.05) by mulching on the total number of tubers for each plant with grass-mulched plots showing the highest value (36) and transparent polyethylene-mulched plots the lowest (19). Also, total weight of tubers per plant at harvest differed (p < 0.05), with grass-mulched plots showing the highest value (2.39 kg), followed by the control (no mulching) plots (1.62 kg), then the coloured polyethylene mulched plots with the red polyethylene-mulched plots as the lowest (0.64 kg). This study recommends the use of organic mulch or no mulching in sweet potato production in this agro-ecological zone as they produced the best yields.
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Obtention of new ornamental leaf variants of giant reed (Arundo donax L.) originated from somatic embryogenesis and their photosynthetic parameters
18-24.Views:1045Giant reed (Arundo donax L.) is a perennial rhizomatous herbaceous plant, it has been widespread all over the world by human activities. It is a 2-8 meter high, polyploid, sterile species (not produce viable seeds), it can be propagated only by vegetative methods (rhizomes, stem cuttings, in vitro biotechnological methods). It has considered promising dedicated energy crop thanks to high biomass production (20-40 dry tons per hectare depending on microclimate), adaptability of different kind of soils and environment and low energy input required for its cultivation. It has been utilized for energetic purposes, biogas/bioethanol production, cellulose/paper production and ornamental purposes. The objectives of the present study were to determine morphological properties of new ornamental giant reed leaf variants originated from somatic embryogenesis and to evaluate their photosynthetic pigment content and photosynthetic activities. The most typical changes was the appearance of different colour (white, yellow, light green) longitudinal stripes on leaves (also on petiole and on leaf blade). It was significant differences (p<0.05) between green and leaf variants in case of photosynthetic pigments content and photosynthetic activity (Fv/Fm value). There was no detectable chlorophyll a or b content in the white bands of leaves and albino shoots. Total chlorophyll content of the white striped leaf variety was more than twice than the light green leaves. Photosynthetic activity and content of photosynthetic pigments also confirm and determine the morphological characteristics and growth habit of leaf variants.
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Selection of the chance seedlings of `Mézes körte' (Pyrus communis L.) from the gene bank of Keszthely
21-27.Views:719We have concluded the selection tests of the `Mézes körte' seedlings planted in the spring of 2006, with special emphasis on the cotyledonary, foliage leaf and the height of plant. Out of the 75 seeds planted in rows, there were 40-45 pieces growing out, so during the first cotyledonary test we had to calculate with almost 40% decay. On 12th April 2006, we recorded some of the important characteristics of the seedlings in their cotyledonary stage which characteristics were important from the point of view of selection (cotyledonary form, cotyledonary length, cotyledonary thickness, cotyledonary colour, cotyledonary petiole length, cotyledonary petiole thickness, cotyledonary petiole colour). The above morphological characteristics are shown in Table No. 1-6. We have also tested the seedling in foliage leaf state, paying special attention on the development stage of the plants (colour of foliage leaf , height of plant). We have completed statistical calculations of the two above mentioned characteristics. The result of that is summarised in Table No. 8-9. The variation coefficient show smaller value in the case of the foliage leaf number (15-32%), while the wider range of spread of the data referring to the height of the plant is shown by the 33-61% CV values. On charts No 4-9. we present the relationship between the height of the plant and the number of foliage leaf, as well as the differences between the two graphs. Based on the above charts and graphs it can be defined that the 40% destruction of the developing seedlings during the period till the next measurement reached 70-80% level. In spite of this however some seedlings showed strong and balanced growth (A44, B42, C25, D16, E5, E39, F38), the further testing and selection of those is to be completed in the future.
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Effect of gelling agents on in vitro development of Amelanchier canadensis ‘Rainbow Pillar’
75-79.Views:463In vitro shoot multiplication responses of Amelanchier canadensis ‘Rainbow Pillar’ were studied on media solidifi ed with different gelling agents. The media were gelled either with 6.8 g l-1 fi brous agar-agar, or 50.0 g l-1 wheat starch, or 20.0 g l-1 Guar gum, or 15 g l-1 Isubgol or 50.0 g l-1 wheat starch mixed with 0.5 g l-1 Phytagel. Shoot cultures were grown for two months, thereafter the multiplication rates (number of newly developed shoots per explant) were counted and the length of shoots were measured. We found that the highest shoot multiplication of Amelanchier canadensis ‘Rainbow Pillar’ occurred on media gelled with Guar gum, while the longest shoots developed on media with Starch. About four-fold shoot number were obtained on media with Guar gum compared to the weakest results found on media gelled with Isubgol. Finally, considering all factors (shoot growth parameters, costs) the most economical gelling agent for Amelanchier canadensis ‘Rainbow Pillar’ was proved to be wheat starch among the tested alternatives which allows a 75.6% cost reduction.
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Composted and natural organic materials as potential peat-substituting media in green pepper growing
31-35.Views:249Peat is the most favourable and usable medium in vegetable and ornamental plant forcing but because of the intensive exploitation peat resources decreased significantly all around the world. As peat-reserves run out the use of pine bark, composts and other organic materials spread in horticultural growing. In this study we compared the suitability of peat-based media to pine bark and two types of composts. We examined the effect of different organic materials on the growth and yield of green pepper (Capsicum annum L., variety Danubia). We found that the most developed plants were grown in peat-based media and pine bark. The average fruit weight was the highest in low moor-high moor peat mixture and pine bark. The plants which were grown in composts fell short of our expectations.
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The fertilization problems of cultivated red- and black currant varieties in Hungary
38-40.Views:235Small fruits have a modest share in the fruit production of Hungarys. Red currant was grown traditionally in home gardens 60-70 years ago. Commercial production was established only in the surroundings of some town. The black currant was unknown until after Wold War II. An important change occured in small fruit production in the 1950s. Socialist countries, which had cheaper labour power, made efforts to meet these demands. In this time we produced 25.000 t.
Presently the country produces 13-15.000 tons currant fruit yearly 60% from this is black currant, which has a better market. It is our own interest to make our currant production more profitable. The currant is the second most widely cultivated soft fruit. Our product is disposed mostly on EU markets.
There was no breeding activity in this field in Hungary earlier. Cultivars used were mostly of foreign origin (W. European; Boskoop Giant, Silvergieter, Wellington XXX, Russian; Altaiskaya Desertnaya, Neosupaiuschaiasya, N. European; Brikltorp, Ojebyn). Besides well-known advantageous this cultivars have also some defects mainly unfavourable—adaptation to climatic conditions, which caused fertilisation problems, reduced the fruit set and uneven growth with decreased yields (Dénes & Porpáczy, 1999). About 140 black currant cultivars were investigated during the last four decades in our variety trials and only four of them were introduced with satisfying yielding capacity (3.5-5.5 t/ha).
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Turkish hazel (Corylus colurna L.) in Hungary: A case study on its forestry potential
48-51.Views:291This study investigates the structure and growth of a 73-year-old Turkish hazel (Corylus colurna L.) plantation in Hungary. The stand had a tree density of 290 per ha, mean height of 18.2 m, quadratic mean diameter of 34.2 cm, and a basal area of 26.7 m² ha⁻¹. The slenderness ratio of 0.55 indicates moderate stability. Diameter distribution was normal (22–53 cm). Crown structure parameters showed moderate correlations with diameter (R² = 0.59 for crown diameter; R² = 0.53 for crown projection area), emphasizing the role of crown traits in yield modelling. An optimal planting density of 434 trees ha⁻¹ was estimated based on the average crown projection area (23 m2). Turkish hazel, with its drought resilience and valuable wood, is a promising species for afforestation in semi-arid climates. However, further research is needed on its management and genetic improvement to support wider application. This study can be considered gap-filling both in a Hungarian and, to some extent, an international context.
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A preliminary study on some features of two new resistant apple cultivars in a multi-row planting system
11-14.Views:910The aim of this work was to report preliminary results on some features of two new resistant apple cultivars (cvs. ‘Galiwa’ and ‘Story Inored’) in multi-row system in the early bearing years of the orchard. Trees were planted in spring of 2015 in double row design (3.5 m+1.4 m x 1.0 m) with planting density of 5357 tree/ha. According to our results cv. ‘Story Inored’ presented more vigorous growth, than cv. ‘Galiwa’. Trees of cv. ‘Story Inored’ reached 2.7 m in the third year, but cv. ‘Galiwa’ could reach only 1.95 meter. Due to late spring frost 100% yield loss was observed in the second year. In 2017 cv. ‘Galiwa’ produced 5.3 kg/tree (25 fruit/tree), while cv. ‘Story Inored’ presented 7.7 kg/tree (50 fruit/tree). Average yield was 28.4 t/ha for cv. ‘Galiwa’, as ‘Story Inored’ reached 41.3 t/ha in the third year. Cultivar ‘Galiwa’ reached the required fruit size (79.1 mm), but its coloration was weaker (43% fruit surface color). Cultivar ‘Story Inored’ can be described with smaller fruit size (69 mm) and higher percentage of surface color (93%).
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Irrigation management of a peach orchard
19-24.Views:886The research field was at Siófok, in Hungary, which is situated in the South East side of Lake Balaton. The physical characteristic of the soil is sandy loam and loam and the peach orchard is irrigated. Mainly Sweet Lady (early ripening), Red Heaven (medium ripening) and Weinberger (early ripening) species were installed. In order to achieve the optimal developement level of trees and maximal yield amount and fruit diameter (Sweet Lady 60–75 mm, Red Heaven 60–70 mm, Veinberger 50–60 mm) continous water and nutrient supply is required. The irrigation modeling was set by CROPWAT 8.0 based on the climatic, crop and soil data inputs of the last 10 years. Based on the results, large amount of water is needed for optimal growth of fruit trees, particularly in the summer months, in case of active ground cover (+) and bare soil (–) as well. The irrigation requirement of a tree was found maximum 4 l/hour in certain cases. This irrigation intensity can be achieved – calculated with 12-hour operating time – by using continuous water NAAN Tif drip tube with 2 l/h flux on 3 atm pressure with 16 mm pipe diameter. If lower irrigation intensity is required irrigation can be controlled by the decreased the operation time.
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In vitro multiplication and hardening of grapevine plants in aeriated media
15-18.Views:378In vitro cultures have widely been used in horticulture for rapid multiplication of new varieties and clones as well as to produce pathogen-free stock material. To improve efficient hardening and transfer in vitro grown grapevine plants were multiplied by cutting them into single-node internodes with the whole leaf. Microcuttings including the shoot tips were rooted in granulated perlite moisted with tapwater under sterile conditions. After 2-3 weeks the rooted microcuttings were supplied by nutrients and hardened by gradual opening and finally by complete removal of the lids of jars or plastic boxes used for growth. Using this method microcuttings of Vitis vinifera cvs. „Chardonnay", „Cabernet franc", „Riesling" and „Sauvignon blanc" and the rootstock varieties Vitis riparia x Vitis cinerea cv. „Barrier" and Vitis berlandieri x Vitis rupestris cv. „Richter 110" formed new roots and shoots and 100% of the tested plants survived the acclimatization procedure. Similar results were obtained when perlite was replaced with rockwool-, or pit-pot blocks. This method may highly increase the efficiency of producing pathogen-free propagating material and new transgenic lines.
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Comparative investigation on Hypericum perforatum L. populations of different origin
56-60.Views:449Widespread application for herbal medicines based on Hyperici herba has been experienced in the last few years, especially in the treatment of depression syndrome. As the wild origins could not satisfy the market demands neither in quantitative nor in qualitative respect, the necessity of the development of intensive growing methods has been raised. In the course of our investigations we intended to clear up the morphological and chemical variability among and within populations of different origin in order to start a new breeding program. According to our data, growth dynamics of populations could be characterised by a logistic curve. According to the time of flowering the populations formed early, middle and late groups. Morphological diversity among populations was measurable in differences of flower length, plant height, different leaf types and plant habit, according to which characteristics groups were distinguished. Generally, the accessions were the most homogenous in plant height (CV: 7-15%), followed by inflorescence-lengths (CV: 11-36%) and the least uniform characteristics proved to be the number of flowering shoots (CV: 14-59%). The greatest morphological heterogeneity was experienced in the accessions of wild origin as it has been expected. In the second vegetation period generally a much better homogeneity was obtained, than in the first year. The most outstanding accessions produced 1.2 t of dry flowers and 4 t of dry flowering shoots, calculating to one hectare area. The individual yields varied on a large scale in each population (CV: 18-70%).
The content of hypericin varied between 1,85 and 9,9 mg/g in 1996, and between 0,18 and 2,70 mg/g in 1997, showing high individual divergences. Flavonoid values -including first of all hyperosid, rutin and quercitrin - reached 17-39 mg/g in the first and 15-20 mg/g in the second year, respectively. Individual and seasonal variation was less than in hypericin. A joint high level of these two compound groups seems to have low frequencies.
The superior population were selected for further breeding.
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Evaluation of generative accomplishment of new apple cultivars in Hungary
11-15Views:720In a two year study, 14 new apple cultivars (cvs ’Gala Venus Fengal’, ’Gala Decarli-Fendeca’, ’Galaval’, ’Jugala’, ’Gala Schnitzer (S) Schniga’, ’Red Cap Valtod (S)’, ’Early Red One’, ’Jeromine’, ’Crimson Crisp (Co-op 39)’, ’Red Topaz’, ’Wilton’s Red Jonaprince’, ’Red Idared’, ’Fuji September Wonder’ and club cultivar) were studied in Eastern Hungary at Nyírbátor. The following parameters were measured: trunk cross sectional area (cm2), harvest time with yield (date, kg/tree, number/tree), fruit size (mm), fruit surface color (%), and color intensity of the fruit surface (1-5). According to our two year assessments regarding, the best generative accomplishment was achieved for the cvs ‘Gala’ sorts trained to slender spindle and the cv ‘Red Jonaprince’ trained to super spindle. According to the trunk cross sectional area the ‘Gala’ sport trained to slender spindle showed the strongest growth among the 4 years old trees, while the cv ‘Fuji SW’ and the cv ‘Jeromine’ presented lower vegetative accomplishment. The ripening time of the cultivars started at the end of August and finished at the beginning of October. ‘Gala’ mutants trained to slender spindle provided the highest yields. Fruit size of all cultivars reached the market required standard, however, cvs ‘Red Jonaprince’ and ‘Red Idared’ produced large fruits over 90 mm. The fruit size of the ‘Gala’ sort was 72–75 mm. Most of the cultivars showed high fruit surface color and color intensity.
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Variety specific integrated fruit production development in order to optimize inner content values
27-31.Views:417In this paper we introduce our results of three years (2007-2009) investigatio ns carried out in the framework of "Research and development in foodstaff chain" - project of Regional University Knowledge Center by the members of Corvi nus U niversity of Budapest - Department of Pomology and. Research Institute for Fru it growing and Ornamentals. Budapest-Erd. The main objectives of the project were the followings: submi ssion of sour cherry candidate suitable for industrial process for state approval; determination of physica l parameter and inner content value changes of sour cherry varieties duri ng ripening; evaluation of health care attributes of sour cherry fruit; ripen ing process description by the colour and the force required to pick fruits of sour cherry. We stated that the optimal begi nning and period of the harvest can be determined wi th the fruit removal force. In this period the fruit growth stops. ju iciness rat io does not change. refractions are approximately equal.acidic content turns to decreasing trend.However the proportion of anthocyan in and polypheno l can still increase. Fruits harvested in this period fulfil a wide range of industrial process opportunities. 'Erdi jubileum ·and candidate IV-3/48 according to their inner content values arc suitable for high quality products (containing real fruit material in high proportion).
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Increment analysis in black locust (Robinia pseudoacacia L.) stand – a case study
106-109Views:505The study on the diameter and volume increment of black locust (Robinia pseudoacacia L.) stand (age 10-27 years, yield class II) have shown that in comparison to the periodic annual increment of the breast height diameter of tree in height class I, trees in height class II have reached 83.3%, while in class III only 43.9. The same relations for volume were found 59.0% and 24.5%, respectively. The mean values of the whole stand were close to those of height class II. According to the distribution of the periodic annual increment of volume between 10 and 27 years of age, 50% of the values were between 2.00 and13.88 dm3, 73% were between 2.00 and19.82 dm3, and 96% were between 2.00 and 37.64 dm3. The range of 13.88–19.82 dm3 had the highest occurrence (24.5%).
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Real-case application of mycorrhizal inoculums on Capsicum annuum L. var. longum cv. Szegedi and Kalocsai
75-79.Views:317The aim of this study was to test the use of commercially available arbuscular mycorrhiza (AM) inoculant Symbivit, a mixture of six species of Glomus spp., in spice pepper field cultivation. The inoculants containing arbuscular mycorrhizal fungi (AMF) was able to establish a symbiosis in the rhizosphere of pepper plants and mycorrhizal inoculation increased fresh and dry weights of shoots of spice pepper cv. Szegedi and only fresh weight of Kalocsai type. There were no significant differences in the root weights due to treatment only in fresh weight of Kalocsai pepper type. Treated plants of both variants exhibited an increase in cumulative crop production compared with control non-treated plants and the growth response of pepper was higher for var. Szegedi than var. Kalocsai. Mycorrhizal inoculation had a great positive effect on external hyphal length of AMF also showing differences in that between Kalocsai and Szegedi variants. The root colonization showed seasonality by treated and non-treated plants. The lowest degree of colonization was observed in June in general and colonization percent increased during vegetative development and there was a slight decrease at harvesting. In conclusion, it can be stated that inoculation with Symbivit containing mycorrhizal fungi could be an integral part of spice pepper production.
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Down-regulation of ethylene production in carnation (Dianthus Caryphyllus L.) by an apple derived ACC-cDNA
101-104.Views:250Transgenic carnations were produced with an apple derived antisense ACC-synthase cDNA. Transgenic carnation regenerants were potted in glasshouse. All transformed plants showed normal growth and were true-to-type. Ethylene production — measured at full opening stage — lowered by 30-60 %, no plant with 100 % decrease was identified. The vase-life has been observed for 5 years. 38 % of the transformant carnations showed a higher a relative value in days by more than 2 days to 6 days. Twenty six plants were found exhibiting the most marked alterations in the tested trait. In these plants ethylene production decreased by 37-67 %, they have longer vase-life (by 4 days or more). Since the fragrance variety 'Bíbor' was the plant material for genetic modification of vase-life, this trait has been conserved after transformation in spite of the fact that the position of transgene integration cannot be directed.
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Morphological characterization of shallot (Allium cepa L. var. aggregatum) segregating populations obtained from natural-outcrossing in Ethiopial-outcrossing in Ethiopia
74-83.Views:356Shallot is a vegetable and condiment crop widely used in Ethiopia and globally. However, absence of improved and adaptable varieties has been the major cause of low productivity. Narrow genetic base of local shallot germplasm owing to vegetative reproduction of the crop, among others, has been the root cause of low productivity. Nevertheless, some plants within the germplasm were observed bolting and producing viable seeds, presenting an opportunity for genetic diversification. Consequently, a germplasm enhancement program was initiated using these naturally outcrossing genotypes where about eighty-one genotypes were generated. The present study was thus undertaken with the objective of characterizing, classifying, and selecting the eighty-one genotypes for future breeding activities. The genotypes were planted in 9x9 simple lattice design with two replications at Debre Zeit Agricultural Research Center (Ethiopia) during the dry (irrigated) season of 2021. The genotypes were evaluated for fifteen growth, yield, and quality traits. Significant variations were observed among the genotypes in terms of bulb yield, bulb height and diameter, total soluble solids, bolting percentage, and bulb skin color. Bulb yield of the genotypes ranged from 31.33 t/ha in DZSHT-79-1A to 9.63 t//ha in DZSHT-45-1A-1. DZSHT-51-2 (207.93 g) was the highest yielder per plant whereas DZSHT-065-6/90 (74.51 g) was the lowest yielder. DZSHT-14-2-1/90 had the thickest bulb (44.69 mm) significantly thicker than twenty two genotypes which had bulb diameter ranging from 28.92 mm to 20.29 mm. DZSHT-81-1/90 was a genotype with the longest bulb height (52.33 mm) while DZSHT-147-1C was a genotype with the shortest bulb (33.12 mm). DZSHT-307-1/90 had the highest TSS (16.78°Brix) significantly differing from DZSHT-002/07 which had the lowest TSS (11.17 °Brix). Dry matter of the genotypes ranged from 12.00% to 22.79%. DZSHT-004/07, DZSHT-111-2-1, DZSHT-41-2B and DZSHT-72-2 had DM% greater than 20% which coupled with greater than 14 °Brix could make them suitable for dehydrated shallots. Among the 81 genotypes characterized 4 (4.9%), 7 (8.6%), 13 (16.1%), 28 (34.6%) and 29 (35.8%) were yellow, golden, light red, red and dark red in colour, respectively. Fifteen of the genotypes had at least 50% bolting plants whereas twenty nine of the genotypes had less than 25% bolting. The results revealed that seven principal components explained approximately 76% of the observed variation. Cluster analysis grouped the genotypes into seven clusters, with the majority falling into three clusters. The study successfully identified genotypes with diverse and important traits and availed both the genotypes and the information for future breeding programs. These genotypes could be used for the development of improved hybrid and open pollinated shallot varieties with higher yield, quality and pest resistance/tolerance attributes.
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Application of the Jerusalem artichoke (Helianthus tuberosus L.), as a plant origin medium additive, during the micropropogation of Ada keiliana
61-64.Views:288A procedure for in vitro propagation of Ada keiliana seedlings are suited for acclimatization, was worked out. M medium was supplemented, with Jerusalem artichoke, as plant origin complex additive. The apply of JAD (1,5g/flask) gave the best response, considering the shoot (29 mm), and the root development (24,9) mm) too. The plantlets with satisfying growth (25-30 mm, 4-5 roots) were transferred in small pine bark: Novobalt peat: coconut fibres: perlit (2:3:1:1) mix, among greenhouse circumstances.
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Production of transgenic carnation with a heterologous 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase bifunctional enzyme cDNA
75-79.Views:254Transgenic carnations were produced with a modified mammalian bifunctional enzyme cDNA coding 6-phosphofructo-2- kinaseffructose 2,6-bisphosphatase. Relative activity of this enzyme determines the fructose 2,6-bisphosphate (fru 2,6-P2) cytosolic concentration. This metabolite — as a signal molecule — is one of the carbohydrate metabolism regulators. The regenerated Dianthus chinensis and Dianthus caryophyllus shoots were selected on MS basal medium containing 150 mg/1 kanamycin. Transgene integration was proven by PCR analysis with cDNA specific primers followed by Southern hybridization of DNA isolated from selected green shoots, which survived on kanamycin containing medium, so 3 D. chinensis and 20 D. caryophyllus transgenic plants were produced. Transgene expression were examined by RT-PCR. Transformed and control plants were potted in glasshouse to evaluate the effect of modified fru 2,6-P2 on development, growth and carbohydrate metabolism.
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Evaluation of hot pepper (Capsicum annum L.) varieties for green pod yield and quality under rain fed production at Teppi, South Western Ethiopia
45-49.Views:383The trials were designed with three replications in a randomized complete block design in order to evaluate the phenological, growth and yield potential of hot pepper varieties such as ‘Mareko fana’, ‘Melka Zala’, ‘Melka Awaze’, ‘Melka Shote’ and local check. Significant difference was observed between growing year and used varieties. The varieties also performed significantly different (p<0.05) for most of the considered traits in the study. The result revealed that varieties ‘Melka Zala’, ‘Mareko fana’ and ‘Melka Awaze’ were scored highest green pod yield of 8.39, 8.71 and 11.39 ton per hectare, respectively. However, ‘Mareko fana’ variety was susceptible to disease attack as compared to other varieties. Therefore, promoting both ‘Melka Awaze’ and ‘Melka Zala’ varieties for widespread production for Teppi and the areas with similar agro-ecological conditions could contribute to boost the productivity of hot pepper. ‘Mareko fana’ could also be used for dry pod purpose due to its attractive color.
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The inheritance and durability of scab resistance in apple progenies
39-46.Views:365In order to select the appropriate parent cultivars and maintain the durability of resistance, it is important to clarify the mechanisms of inheritance of scab resistance depending on the parents. It has been known that the progeny segregation ratios based on scab-resistance do not depend only on the genotype of the resistance locus but also on the genetical makeup of the donor and recipient parents as well as on the susceptible parent.
The aim of this study has been to demonstrate what factors in the Vf, Vr and VA scab-resistant cultivars — combined with susceptible and resistant parents — affect the inheritance and durability of resistance in seedlings in their first 4 year's growing four years' growth. After inoculating apple seedlings sown in 2001 with the suspension of Venturia inaequalis (Cke.) Wint. in the greenhouse, we studied the segregation ratios of the progenies into reaction classes. Seedlings showing resistance in the greenhouse were also evaluated for scab-resistance after they had been moved to the field and had naturally been infected with the pathogen in 2002 and 2004.
The majority of our results obtained in the greenhouse test, similarly to earlier experiences, have not justified monogenic inheritance at the phenotypic level. The effect of susceptible parent cultivars on the segregation ratio of progenies have become apparent again. The high infection rate of seedlings in the field trials, which had previously exhibited varying degrees of resistance in the greenhouse test, has raised concern. Our data has raised further doubts, concerning the durability of Vf resistance in Hungary. It is assumed that the composition of natural field populations of Venturia inaequalis in Szigetcsép has changed. The complexity of Vf resistance has been confirmed. The high infection rate in the progenies derived from Vf resistant cultivars draws the attention to the importance of utilizing additional sources of resistance.
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Investigation of the in vitro regeneration of mericlones in the caribe variety of carnation
87-89.Views:235In vitro culture conditions were experimented for the relatively sensitive, but very esthaetic "Caribe" variety of carnation with uniformly dark violet flowers. Regeneration of new plants from shoot apex meristems can be significantly improved by the combined addition of very low amounts of indolebutiric acid, benzyladenine and gibberelic acid, dissolved in the Murashige-Skoog nutrient medium. Callus formation as a prerequisite for the induction of somaclonal variability can be achieved successfully with certain molar ratios between 2,4-dichlorophenoxyacetic acid and benzyladenine. Acclimation of the obtained mericlones to the ex vitro conditions was also evaluated.
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Effects of irrigation frequency and repellent plants on false codling moth (Thaumatotibia leucotreta) infestation, growth, yield, and quality of sweet pepper (Capsicum annuum L.)
34-40.Views:96Sweet pepper (Capsicum annum L.) is an important vegetable and spice crop grown worldwide for its pungency, which is derived from high concentrations of capsaicinoids. Despite the economic importance of sweet pepper as an export vegetable in Kenya, its production is currently constrained by the false codling moth (FCM) (Thaumatotibia leucotreta) and inadequate management strategies for the pest, among other factors. This study, therefore, evaluated repellent plants (Artemisia (Artemisia absinthium), Lavender (Lavandula angustifolia), and Spearmint (Mentha spicata) under different irrigation frequencies to control FCM. A split-plot factorial greenhouse experiment was conducted at Kenya Agricultural and Livestock Research Organization, Muguga (trial 1) and the Horticulture Research and Teaching Field, Egerton University, Kenya (trial 2). Irrigation frequency at three levels (irrigating once a week, irrigating twice a week and irrigating thrice a week) was the main-plot factor, while repellent plants at four levels: sweet pepper (Capsicum annum L.), artemisia (Artemisia absinthium), lavender (Lavandula angustifolia) and spearmint (Mentha spicata) constituted the sub-plot factor. Irrigating thrice or twice a week and using lavender as a repellent plant significantly reduced false codling moth infestation by 90%, increased yield by 28% and improved fruit quality in terms of fruit collar diameter and total soluble salts (TSS). The findings demonstrate that integrating lavender repellent plants with frequent irrigation can be adopted as an eco-friendly management strategy for FCM and enhancing sweet pepper yield and quality.
