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Mutation induction in sweet basil (Ocimum basilicum L.) by fast neutron irradiation
30-38.Views:350Basil species are highly sensitive to exterior environmental conditions and its consequences lead to great economic and agronomic losses. In this research, a mutation method was optimized out for creating a new variety of Ocimum basilicum L., which could tolerate the extreme/extraordinary climatic circumstances or biotic stresses, such as fungal diseases. Fast neutron irradiation was performed on the Hungarian commercial variety seeds with doses of 5 to 60 Gray and grown into fully developed plants. Numerous phenotypical changes like deformed congestion, leaf mutation, and low growth occurred, especially at higher dosages. Then to confirm whether the plantlets had mutation or not, and to detect the molecular variation and relationship, fingerprinting profiles of the developed mutant regenerants and donor plant have been assessed using ISSR markers. 115 loci were yielded, ranging from 0.2 to 1.5 kb, out of which 110 loci were polymorphic in nature, representing 95.6% polymorphism. The most suitable primer to determine the genetic diversity within the Ocimum species was the UBC-856 with 0.42 PIC and 4.1 MI values.
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Actual state of research concerning vitamin C as reflected in the literature (Review article)
7-15.Views:206Vitamin C (L-ascorbic acid) being essential for many living organisms, including man, became once more into the focus of interests because of its numerous physiological effects. Its anti-scurvy and anti-oxidant properties have already been recognised since long in the human body, but it turned out gradually that it has many other functions. In plants, its primary importance is defense against the photo-oxidative stress.
The present review is intended to reveal some details of the artificial synthesis of vitamin C. Emphasis is put on the metabolism of L-ascorbic acid in higher plants. Biosynthetic processes, translocation and accumulation are discussed in detail on the basis of recent results published in the scientific literature.
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Impact of sodium-selenate on the growth of radish (Raphanus sativus L.) seedlings in vitro
113-115.Views:192Selenium (Se) is an essential trace element for animals, microorganisms and some other Eukaryotes. It has become increasingly evident that Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Although it is well known that some species among higher plants are able to accumulate selenium in their tissues, but others are not able to do so, and there is evidence that selenium is needed for the growth of algae, meanwhile the question of essentiality of Se in vascular plants is unresolved. We aimed to study the in vitro growing and to characterise some physiological properties in radish (Raphanus sativus L.) seedlings treated with 0 to 200 mg/1 sodium-selenate. The results showed that lower (2 mg/1) concentration sodium-selenate increased the biomass as well as the total antioxidant capacity of seedlings. The seedling's selenium content showed linear correlation with the sodium-selenate content of the medium.
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Microclimatic studies on different aged apple plantations
7-11.Views:233The purpose of measuring parallel canopy and out of canopy microclimates was to find out in what extent climatic parameters measured in different aged canopis differ from each other and from the values characteristic to out-of-canopi areas. The importance of phytoclimatic researches seems to lie in the fact that if the reactions of fruit trees towards meteorological elements are continuously followed, we have the possibility to provide growers with information. These pieces of information are like defining the optimum time of phitotechnical interventions (summer pruning, sorting sprouts, thinning fruits, etc.), the necessity of applying mulching, defining the method and time of irrigation and applying plant protection activities. By means of phytoclimatic researches, it is possible to react to unfavourable meteorological impacts within a certain extent. It is also possible to successfully reduce the risks of late spring and early autumn frost damage, as well as the risks, content and measure of experienced heat and water stress conditions by finding out about the physical characteristics of the canopis' internal area.
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Comparative study of sour cherry samples for their anthocyanin content measured by tools of the laboratory and by portable equipments
29-31.Views:280The inner quality of fruits depends on many components. The analysis of pharmacologically important fruit species as the sour cherry consumed as a common food is an expensive task, mostly requiring an equipped laboratory. In sour cherry, one of its valuable components, the anthocyanin content deserves special attention. A portable analytical implement, which has been developed by our team measures the anthocyanin content on a scale of 1024 PharMgrades. The system is a member of the UVEX family of implements working with a microprocessor and performs the measurement quickly. The portable variant (UVEX-ML-1) does not require much training, the laboratory variant (UVEX UL-1) performs a large quantity of tests under modest laboratory conditions. Preparation of the samples is easily done, the necessary elements are available in the trade. The reagent is easily stored and dosed. The system was tested and proved to be of sufficient precision and the result showed acceptable variance according to the checks performed by a spectrophotometer in the laboratory.
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Drought stress monitoring by laboratory and satellite spectral methods in an apple orchard
7-9.Views:248An orchard can be examined on the basis of spectral data, using such methods with which the reflected radiation can be divided into a large number of (several hundreds) small spectral channel (some nm). Based on the spectral characteristics of the canopy, or the different index numbers calculated from hyperspectral data the water supply conditions of foliage can be well characterized. The research site is an intensive apple orchard, which located in Debrecen University, Centre for Agricultural and Applied Economic Sciences, Farm and Regional Research Institute at Pallag. During our experiments the evaluation of spectral, non-invasive measurement method are carried out for detecting stress symptoms caused by drought. Furthermore, MODIS NDVI time series data were analyzed for orchards situated in North-Eastern part of Hungary in drought effected and wet years in order to detect differences. Significant differences in NDVI values were detected after the end of June.
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Effect of over tree cooling irrigation on ‘Bosc’ pear orchards microclimate
153-156.Views:358Irrigation in some countries is a horticultural practice mainly used only to supply water. At the same time the use of microsprinklers have a powerful infl uence on the changes of temperature in orchards. When the air’s temperature is high (about 20 °C or higher) the evaporative cooling irrigation signifi cantly decreases the plants’ surface temperature and air temperature. The cooling effect is stronger when the air is dryer. By using cooling irrigation regularly, canopy temperature can be decreased so that the beginning of blooming can be delayed. Also if the blooming is early and frost probability is high, serious damages can happen in orchards. The benefi cial effect of cooling irrigation is the temperature reduction and frost protection. In March 2010, one month earlier than the expected blooming an irrigation system was established to produce anti-frost treatment and regulate the micro-climate of a Bosc pear orchard which belongs to the University of Debrecen (Hungary). The objective of sprinklers was to cool the air by increasing water evaporation and relative humidity. The position of the micro-sprinklers was planned in three levels (around the tree trunks, a few cm near to the soil surface, in the crown region and above the crown, a half meter higher). The results showed that the water sprayed in the orchard by micro-jets infl uenced decisively the temperature of the plantation. At higher temperatures (around 20 °C), the drop of temperature may attain 5–7 °C. A low relative humidity of the air may increase the relative effect. When water was applied at intervals of 15 minutes for ten times a day from 8 am to 18 pm, the air, fl owers and bud’s surface temperature could be kept low. At certain days when the temperature was higher than 10 °C, irrigation was used at night time in similar 15 minutes intervals, from 18 pm and 6 am. The beginning of bloom could be delayed for more than ten days. The Bosc pear variety blooming dynamics was characterized by a logistic curve in the treated as well as in the control plot. In the treated plot, the curve was steeper than in the control one in spite of the equal temperatures measured in the plots. Under Hungarian climatic conditions, the method was successfully used to delay blooming dates. The main result was the diminution of the frost damage in the spring that assured pears yields.
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Flower density and winter damage of apricot and peach varieties
53-56.Views:258Hungary is located on the northern boundary of economical apricot and peach production. The present assortment of varieties and the actual, not adequately selected growing sites cause a permanent risk of winter and spring frosts in their cultivation. The field observations are performed at Debrecen, the Experiment Station Pallag, on 20 apricot and 21 peach varieties. The flower density among the varieties attained 3-4-fold differences. Three categories have been suggested for both species. The density was inferior in Hungary established varieties compared with the new varieties of foreign origin. The minimum temperatures of January 9, 2009 was –17,6 °C , and of December 21, 2009 also –17,6 °C. In some varieties the damage of buds attained 100%. For estimating the yield security, we need to consider also the flower density and the frost damage together. For a mediocre yield, we need a flower density in both species of at least 0.2 living bud/cm. The results confirm the statement that in Hungary, the revision of growing sites is indispensable in order to develop a profitable and competitive apricot and peach growing industry.
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Sensory evaluation of white asparagus (Asparagus officinalis L.) hybrids
51-55.Views:327Comprehensive system of sensory evaluation was developed to obtain information on the different varieties by determining asparagus quality parameters. 9 sensory characteristics were estimated to describe flavour differences. The tests were carried out in 4 varieties of white asparagus (Asparagus officinalis L.) grown on brown humus sand soil by etiolate method in ridge cultivation. The experiment was performed at the University of Debrecen in Debrecen-Pallag. To increase the Hungarian asparagus consumption, a classification of varieties
regarding to asparagus flavour is necessary. In our experiment it could be stated that there are strict correlations (0.892) between the sweetness – total impression and between lacks of unpleasant aftertaste – total impression (0.870). The result of surveys can lead to higher popularity of the asparagus. These results are presented in order to recommend the varieties to producers for a successful marketing. The taste of asparagus is mostly influenced by variety, soil type and growing conditions. In our experiment, ‘Vitalim’ hybrid exceeded by several sensory parameters. This hybrid scored 788.38 over 900. The elder population showed more interest in asparagus consumption than younger ones. 50% of the participants are able to pay only 500-600 HUF/kg. It could be realized to ensure the proper growing place, hybrid and direct trade. Marketing and more information is necessary to increase the popularity of this vegetable among the younger generation. -
Security of growing habits and bud formation of German sour cherry varieties
45-48.Views:235Intensive sour cherry production is concerned to find the most productive varieties under special growing conditions. High planting density, adapted to manual picking or on the other hand to mechanical harvesting. Almost as important is the prolongation of the ripening season by enlarging the choice of varieties. Unfortunately, three of our four leading commercial varieties are ripe almost at the same date.
Therefore, new varieties ought to be examined thoroughly. In the Pallag Experimental Station of the Debrecen University, five European varieties have been grown (’Schattenmorelle’, ’PI-SA 12,100’, ’Jade’, ’Gerema’, ’Achat’) and a Hungarian one, ’Debreceni bôtermô’ used as a check for the experiments to compare their growing and yielding habits in 2010. The plantation was three years old, standing on Prunus mahaleb rootstocks, in high density (5 x 2 m) and trained to slender spindle crowns. The results are proving that some of the varieties in
question are suitable to prolong the harvesting season. Growing habits and yields of the varieties related to the variety ’Debreceni bôtermô’ were similar or even better as ’Jade’, ’Gerema’ and ’Achat’. The ’Jade’ excelled with its vitality and ’Gerema’ with its generative character. Further studies are expected to prove the utility of
one of them at least to enlarge the ripening season on the fruit market. -
Preliminary study on micro area based spatial distribution of Monilinia fructigena in an organic apple orchard
19-21.Views:209In this study, we aimed to report a preliminary study on micro area based spatial distribution of Monilinia fructigena in an organic apple orchard. Results showed that number of symptomatic fruit ranged between 22 and 42 in 2013 and between 25 and 35 in 2014. Number of asymptomatic fruit ranged between 111 and 187 in 2013 and between 119 and 167 in 2014. Disease incidence of fruit ranged between 19.7 and 23.2% in 2013 and between 19.1 and 26.5% in 2014. Disease aggregation index ranged between 0.111 and 0.335 in 2013 and between 123 and 401 in 2014. Three of the four trees showed significant within canopy aggregation of disease for fruit brown rot symptoms in both years. However, the remaining one tree exhibited random patterns during both years. Disease aggregation indicated a disease spread by fruit-to-fruit contact and/or an aggregated
pattern of insect damage. -
Susceptibility of fruit of some plum and apricot cultivars to brown rot
53-55.Views:288In this three-year study, incidence of brown rot (Monilinia spp.) on fruit of plum and apricot cultivars were evaluated in Kecskemét, Hungary. Results showed that most plum and apricot cultivars expressed symptoms caused by Monilinia spp, graded between 2 and 4 (10–75%) by the end of the summer in 2008–2010. Assessments on plum showed that only cultivars ‘Besztercei’, ‘Silvia’ and ‘Tuleu gras’ were partly tolerant to Monilinia spp., while the most susceptible cultivars were ‘Bluefre’ and ‘Stanley’. The most tolerant apricot cultivars were ‘Borsi-féle kései rózsa’, ‘Piroska’, ‘Pannónia’ and ‘Magyar kajszi’ while the most susceptible ones were cvs. ‘Budapest’ and ‘Mandulakajszi’. Susceptibility classes showed that only one plum (’Silvia’) and one apricot cultivar (‘Borsi-féle kései rózsa’) were available with low susceptibility.
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Specialities of the vegetation start and level of primary fruit set affect fruit quality
17-22.Views:189Thinning is a h ighly crucial point of the apple production technology. According to results of numerous studies the earliest thinning is deemed to have the best amending effects. There can be considerable difference between trees of the same cul tivar and age in a plantation in respect to their flowering, in the numbers of fruits set and also in their canopy volume. Thus it can be crucial -just l ike in the case of pruning- to establish a fruit thinni ng su i ted for specif ic characteristics of actual trees. This experiment was established to examine how does primary fruit set (fruit load before thinning) and further on specific (fruit/TCS cm2) and absolute (fruit/tree) fruit-load of the trees affect quality attri butes at harvest. Our experiment was establ ished in a plantation on medium-tight loamy soil in north-east of Hungary. Harvest date was determined w ith the joint observation of the calendar date, starch-index, flesh firmness, background colour and fruit weight. Three fruit-load levels were established based on local experience and on data of several years, I Ot/ha lower and higher besides the advised optimum yield in the same orchard with slender-spindle shaped 'Gala must' cultivar standing on M9 rootstock in 1m x 3,8 m spacing. In the establishment of the 15, 25 and 35t/ha fruit-load levels on 20-20 apple trees total number of fruits set was counted at each tree. After this number of apples due to be removed was defined using an objective index on the basis of trunk cross sections (fruit/TCScm2) (Lafer, 1999). The following attributes were measured: weight (g), flesh firmness (lb/cm2) total soluble solid content (Brix %) and total titrate-able acid content (g/J).From the data on sugar and acid content quality index (Pomona value) was determined (Thialult, 1970). We could ascertain, that in an orchard, of the same aged but in concern to trunk cross sections somewhat different trees besides the specific index (apple/TCS cm2 the absolute fruit load (fruit/tree) can also be an important data, that has considerable effect on the internal quality. Secondly we could observe, that higher level of fruit load before thinning (primary fruit set) negatively affects quality index of the apples irrespective of the specific fruit load level (fruit/cm2 TCS) set later. Results underlines necessity of the earliest chemical thinn ing.
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The effect of day and night temperatures on apple skin colour formation
21-25.Views:247The colour of fruits is considered to be an important quality indicator. Saleability greatly depends on how well covered the colour is of the specific type of fruits. It is a well-known phenomenon by growers that apples get nicer colours in one year while in other years the basically red and green colour cultivars can be differentiated only by morphological characteristic features. Cover colour is one of the phenometric variables and it is a well-known fact that significant differences can be experienced year by year. The experienced oscillation can be the cause of inappropriate water- and nutriment supply, however it can be the result of some kind of plant disease, extremely high or low temperature, setting rate above the average and outstanding fruit density. In the present examination it is postulated that the degree of cover colour is mostly influenced by day and night temperature. Therefore, our study aims to find out whether it is true or not. Cover colour belongs to those phenometric characteristic features, only the final value of which is taken into consideration; due to their nature of establishment or forming time it seems useless to follow closely the change in the time of vegetation. However, determining the start of colouring and knowing the dynamics of full colouring could carry very important information for growers. If it is possible to determine the curve describing the time change of colouring, we have a possibility to estimate it by means of enviroment variables. So it is also possible to model pigmentation in the future. Knowing this, colouring irrigation could be made more efficient in the future. For this, as a first step, it is inevitable to find out what the relationship is between the main meteorogical variables, namely day and night temperature and the difference between day and night temperature, and colour cover. In this study we summarize and show these interrelations.
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Some aspects of disease management of cherry leaf spot (Blumeriella jaapii) with special reference to pesticide use
45-49.Views:501In this review, some aspects of disease management of cherry leaf spot (Blumeriella jaapii) are summarised with special reference to pesticide use. In the first part of the review, we show the non-chemical control approach (e. g. removal of fallen leaves, planting resistant cultivar) against leaf spot. In the second part of the review, the effect of pesticides including fertilizers (urea) and fungicides on cherry leaf spot are discussed. Special attention are given to the fungicides of copper, dodine, captafol, captan, benomil, chlorothalonil, sterol demethylation inhibitors (e.g. fenarimol, fenbuconazole, myclobutanil, tebuconazole), and strobilurins about their effectiveness against cherry leaf spot. In the final part of the review, possibilities of cherry leaf spot control are discussed in integrated and organic cherry orchards.
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Effect of a nanotechnology-based foliar fertilizer on the yield and fruit quality in an apple orchard
29-32.Views:557Nutrient management is a determining element of the technology in fruit production. Significance of foliar fertilization has been increased continuously over the last years, as it can improve directly the vegetative and generative performance of the trees. In this study we aimed to evaluate the effect of a nanotechnology-based foliar fertilizer (Bistep) with 1, 3, and 5 l/ha dosages on the yield and fruit quality parameters in an apple orchard during 2016 and 2018. According to our results, crop load increased with 29% in the third year of the experiment with the application of 5 l/ha Bistep treatment compared to the control treatments. Fruit weight was also improved in each year, as values of fruit weight in all treatments were higher than the control one (3.0-13.0% growth). Fruit surface color increased with 2-18% due to the foliar fertilizer. During the three years, leaf length was 9.5-9.9 mm on the control trees, as 9.8-10.4 mm was measured on the fertilized ones. In conclusion, yield and fruit quality can be improved in apple orchard using a nanotechnology-based foliar fertilizer.
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Possibility of stevia (Stevia rebaudiana B.) production in Hungary
29-32.Views:283Artificial sweeteners have harmful effect on human health, so it is great interested in stevia extract. Our experiment was aimed to show the possibility of inland production of stevia. Different plastic mulches were used (black and white) on raised bed and were compared to uncovered (control) plots for yield and state of health of plants. Furthermore we evaluated the depth of cuttings (low cutting until the 6th double leaf; normal cutting until the upper ⅓ of the plant) on the yield depending on the covering method. The plants were transplanted on 9 of May, 2014 on raised bed, 3 rows on it, with 33x25 cm spacing. According to our results, the black plastic mulch produced the highest yield, which can be explained by suppressing effect on weeds, furthermore it kept the soil warm, moist and protected the lower leaves from soil wetness. But, the white sheet mulch could not eliminate weeds around the plants. The total biomass on the black plastic sheet covered plots was the highest, nearly 1000 g pro plant by low cutting. On the control plots the fungi infection reached about 25-30%, which caused leaf falling of plants, decreasing of yield by the end of vegetation period. To summarise, stevia production is possible in Hungary, but it is important to pay attention to the balanced soil moisture and low humidity in the leaf area. It is suggested to cover the soil with plastic sheet or organic materials, such as bark and chippings.
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Hyperspectral data in water stress detection
15-17.Views:185Remote sensing methods are applied widespread to investigate large land fields. Within these methods the status of certain vegetation can be determined based on the reflectance spectra of the chlorophyll, in order to support agriculture, forestry and the evaluation of soil pollution. The main aims of our study were to determine and validate the reflectance spectra of fruit tree species, in order to facilitate the identification and evaluation of stressed fruit trees in orchards.
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Nutritional aspects of producing fruits organically
69-74.Views:345Recent interest to avoid use of agrochemicals in fruit growing to safeguard environmental and human health has stimulated interest in organic fruit production (OFP) all over the world. Organic production requires a holistic approach to agricultural ecosystem management. Because of the perennial nature of orchards, this is not a great departure from conventional orchard management, except that corrective techniques are limited primarily to naturally derived materials. Organic mineral nutrition management hinges on two principles: 1) practices that lead to the build up and maintenance of soil that is biologically active and high in organic matter; 2) supplementing the mineral nutrients provided by the soil with fertilizers from approved sources. Organic orchards should be sited on land with superior soils and preplant soil preparation to increase organic matter and correct any sub-optimal soil characteristics. For successful organic fruit growing the following statement should be considered:Weed management is critical to reduce competition for nutrients and water. Soil and leaf analysis provide the basis for correcting mineral nutrient deficiencies or imbalances in organic production. It may be necessary to use a number of strategies to supply mineral nutrients over the life of the orchard. The slower, natural methods applied require a management approach that is simultaneously patient and dynamic.
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Micro area based spatial distribution of apple scab in an organic apple orchard
21-23.Views:227In this study, the objective was to report a two-year investigation on micro area based spatial distribution of apple scab in an organic apple orchard. Results showed that number of symptomatic plant part ranged between 587 and 623 on leaf and between 46 and 78 on fruit for an individual tree. Number of asymptomatic plant part ranged between 1034 and 1321 on leaf and between 119 and 193 on fruit. Disease incidence ranged between 35 and 40% on leaf and between 27 and 33% on fruit. Disease aggregation index ranged between 0.115 and 0.298 on leaf and between 0.117 and 0.221 on fruit. Three of the four trees showed significant within canopy aggregation of disease for leaf apple scab symptoms in both years. For fruit apple scab, two of the four trees showed significant random patterns in both years.
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Branching features of apple cultivars in integrated and organic production technology
11-14.Views:301In our study, vegetative characteristics of 39 apple cultivars were evaluated in environmentally friendly production systems. Numbers of the branches of the central leader in different high zones were shown. According to our results, number of the branches of the axis was probably larger in the integrated production system, compared to the organic one, which is related to the conditional status of the trees. Based on our experiences training and maintaining canopies in integrated system was easier, as relative more extensive canopies were needed in organic farming.
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Leaf number and plant height of three Canna x generalis cultivars
22-24.Views:254The aim of this three-year study was to evaluate the effect of three treatments (thiophanate methyl, charcoal dust, and control) on leaf number and plant height on three Canna x generalis cultivars (City of Portland, Richard Wallace and Robert Kemp). Results showed that the 3-year means of leaf number ranged from 13.9 to 23.1 for the three cultivars. The largest leaf numbers were for cultivar Robert Camp (23.1 in 2010) and the lowest for cultivar City of Portland (13.9 in 2011). Thiophanate methyl treatment produced the highest number of leaves and the values were significantly different at P< 0.05 from the control for all years and for all cultivars. Numbers of leaves in charcoal dust treatments were larger than the control treatments but it was not significantly different. Results on plant height ranged from 69.2 to 160.5 cm for the three cultivars. The largest plant height was for cultivar Robert Kemp (160.5 cm in 2011) and the lowest for cultivar City of Portland (69.2 cm in 2011). Thiophanate methyl treatment produced the highest plant height and the values were significantly different at P< 0.05 from the control for all years and for all cultivars. Plant heights in charcoal dust treatments were larger than the control treatments but it was not significantly different.
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Induce the plant resistance to pathogen infection
89-93.Views:521Systemic acquired resistance (SAR) is a mechanism of induced defense that confers long-lasting protection against a broad spectrum of microorganisms. Salicylic acid (SA) is the signal molecule which is required for induce SAR and is associated with accumulation of pathogenesis-related proteins, which are thought to contribute to resistance. SA paly vital role in some related resistance gene expression in plant cell which have direct or indirect effect on pathogen growth as SA has direct toxicity for pathogen and in the same time has stimulation effect for some enzyme related to reduce the oxidative burst.
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Vegetative parameters of apple cultivars in integrated and organic production systems
15-18.Views:244Success of apple production is highly influenced by the applied production system and the planted cultivar. In this paper growing characteristics of 39 apple cultivars were studied in integrated and organic production systems. These kind of parameters are less studied in the cultivar and training system examinations, although they have huge effect on the training and maintaining of canopy, on the pruning necessity, ultimately on the production costs. According to our results the thickness of the central axis of apple trees showed significant differences between the integrated and the organic systems. Axis of the trees with lower trunk thickness tapers more slightly in the integrated production system, than in the case of the trees with thicker trunk in the organic system. Thicker axis is not accompanied by thicker trunk, namely the thickness of the central leader starts to decrease stronger in the organic production system, compared to the integrated one.
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Evaluation of fruit tree transpiration with heat flux sensor
11-13.Views:195One of the biggest professional challenges of the following years is to develop the water resource management for apple trees. In order to know the water consumption of plants, some traditional and modern methods could useful. In our research the sap flow measurement of the plants were carried out. We have analyzed the transpiration properties of the trees in the investigation period. In order to compare the transpiration of the trees, the leaf areas were scanned and evaluated. Beside these measurements, soil water properties were determined by tensiometers, thus it makes possible to observe the soil-plant-atmosphere system.