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Methodology adaptation and development to assess salt content dynamics and salt balance of soils under secondary salinization
199-206Views:142The effect of irrigation with saline water (above 500 mg L-1) is considered a problem of small-scale farmers growing vegetable crops with high water demand in the hobby gardens characteristic of the Hungarian Great Plain. In order to simulate the circumstances of such hobby garden, we set up an experiment including five simple drainage lysimeters irrigated with saline water in the Research Institute of Karcag IAREF UD in 2019. We regularly measured the electric conductivity (EC) of the soil referring to its salt content and the soil moisture content with mobile sensors. Before and after the irrigation season, soil samples from the upper soil layer (0-0.6 m) were taken for laboratory analysis and the soil salt balance (SB) was calculated. The actual salt balance (SBact) was calculated of the upper soil layer (0-0.6 m) based on the salt content of the obtained soil samples. The theoretical salt balance (SBth) was calculated by the total soluble salt content of the irrigation water and leachates. During the irrigation season, we experienced fluctuating EC in the topsoil in close correlation with the soil moisture content. Based on the performed in-situ EC measurements, salts were leached from the upper soil layer resulting in a negative SB. Combining SBact and SBth of the soil columns of the lysimeters, we estimated the SB of the deeper (0.6-1.0 m) soil layer. We quantified 12% increase of the initial salt mass due to accumulation. We consider this methodology to be suitable for deeper understanding secondary salinization, which can contribute to mitigating its harmful effect. By repeating our measurements, we expect similar results proving that saline irrigation waters gained from the aquifers through drilled wells in Karcag are potentially suitable for irrigation if proper irrigation and soil management are applied.
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Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro
51-55Views:90Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.
Results of potato production depend on tolerance/resistance to abiotic stresses. In many cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.
In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.
There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.
Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.
In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase -
Energy crops on less favoured (alkaline) soil
115-118Views:99The reduction in fossil energy and row material sources induces growing demand for renewable resources. The growing demand for herbal raw materials has land use impacts as well. One way to reduce the conflict between the food and energy crops can be the utilization of less favored areas by growing energy crops. Among the potentially available areas for this purpose the salt affected soils (SAS) occupy a significant territories. SAS with structural B-horizon (meadow solonetz soils) represent the most wide spread group of SAS in Hungary. About half of these soils have been reclaimed and used as arable land and the remaining 50% are used as grassland. Sweet sorghum production for manufacturing of alcohol production was investigated in a long term amelioration and fertilization experiment on a salt affected soil (meadow solonetz). By means of regression analyzes the effect of sodium content of the soil and increasing mineral fertilizer doses were studied. According to the multiple regression analysis only the effect of nitrogen fertilizer was significant. On the solonetz type salt affected soil the effect of water soluble salt content of the soil was not significant, but there was a closer correlation between the ammonium-lactate sodium content and the yield of sweet sorghum. The maximum green mass was 45–50 t ha-1, in the case of low Na content and high level of nitrogen fertilization.
In order to quantify the potential yield of natural grass vegetation the relationship between the soil forming processes and the grass vegetation
was investigated. Beyond the different forms of Na-accumulation, the spatial pattern (mosaic-like characteristic) is also an inseparable feature of salt affected soils. The difference in the water regime, caused by the micro-relief is the main cause of variability. The run-on water keeps the deeper parts of the catena position wet longer. The wet situation causes more intensive leaching. In the low-laying parts of salt affected soils species preferring wet situations (mainly Alopecurus pratensis) are in majority. On the higher parts of the micro-relief species tolerating dry situations (mainly Festuca pseudovina) are dominant. The yearly grass production of low laying areas can be 4–7 t ha-1 but because of prolonged wet conditions the grass is not grazed and mowing can only be in old state. This old grass is not proper for feeding, but it may be suitable as energy plant. -
Evaluation of chickpea (Cicer arietinum L.) in response to salinity stress
105-110Views:201Soil salinity is a severe and expanding soil degradation problem that affects 80 million ha of arable lands globally. Chickpea (Cicer arietinum L.) is very sensitive to saline conditions; the most susceptible genotypes may die in just 25 mM NaCl in hydroponics. Approximately 8–10% yield loss in chickpea production is estimated due to salinity stress. However, it is still not established why chickpea is so susceptible to salt affection. Salinity (NaCl) impedes germination of seeds, though chickpea varieties considerably differ from one another in this respect. Some chickpea genotypes are more tolerant in the stage of germination, tolerating even 320 mM NaCl. The reasons of this variation are unrevealed; there is a shortage of knowledge about the germination abilities of chickpea genotypes in saline conditions. Nevertheless, the effect of salt stress on vegetative growth can be analysed in hydroponics, in pot or field conditions, regardless the experimental environment, the ranking of genotypes regarding salt resistance is coherent. Chickpea genotypes can be different in their ability to retain water, maybe under salt affection; the more salt tolerant lines can maintain higher water content in the shoots, while the more sensitive ones cannot. The identification of salt tolerant chickpea landraces based on developing genetic variability is a suitable strategy to combat against salinity problems arising in arid and semi-arid areas.
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Effect of soil-compost proportion on the abiotic and biotic parameters of soilplant system
99-104Views:95The environmental awareness, coming to the front in the 21st century, motivates us to supply the plant nutrient demand (in point of the plant, the environment and the human health) with natural materials.
Composting is known since the beginning of civilization. We came to know more the processes of composting as a result of last decades’ research, but numerous unexplained questions remained up to this day. The good compost is dark gray or brown, and it should not create an odor. It has aggregate structure, and it’s pH is neutral. Compost is soil-like (Fehér, 2001), nutrient-rich material, which contains valuable nutrients extracted from soil, so if we recycle this, we can decrease the chemical fertilizer and other (example: mineral energy) expenses.
The reason of that we chose the more accurate cognition of compost utilization is to do more effective the site-specific nutrient supply. This increases the average yield and the quality of yield. Besides we can decrease the harmful effects, which endanger the plant, the environment, and the human body.
During the compost utilization experiment we blended the acid sandy soil with compost in 4 different volumetric proportions (5 treatments) than we set the pots randomized. The advantage of this method is that we can provide equal conditions for plants so we can measure the effect of treatments correctly. Our experimental plant was ryegrass (Lolium perenne L.), that grows rapidly, tolerates the glasshouse conditions, and indicates the effect of treatments well. After the harvest of ryegrass we measured the fresh and dry weight of harvested leaves and the total C-, N-, S-content of the dry matter and of the soil, we examined the pH and the salt concentration of soil as well.
Our aim was to study and evaluate the relations between the compost-soil proportion and the nutrient content of soil and plant. In our previous experiments we confirmed (based on variance analyses) that the compost has a beneficial effect on soil and increases the nutrient content of the soil (Szabó, 2009). But it’s important to appoint that the compound of compost is seasonally change: in winter the selective gathered municipal solid waste contains salt that were applied for non-skidding of roads, but salt has a negative effect to the plant. We proved that in our experiment the 25/75% compost/soil proportion was ideal for the plant. This content of compost effected 6 times higher green matter weight compared to the 100% sandy soil. -
Exogenous salicylic acid treatments enhance tolerance to salinity of wheat (Triticum aestivum) plantlets
34-38Views:118Salt stress, an abiotic stress, determines modifications of some biochemical indicators, like, antioxidant enzymes, proline (amino acid
accumulate in higher plants under salinity stress) content, and some physiological processes including: plant growth and development. In
this paper we studied the influence of exogenous treatment of wheat seeds, with 0.1 mM salicylic acid (SA) solution, in the plant response to
salt stress. The treatment was applied by presoaking the seeds in the treatment solution for 12 hours before germination. The results showed
that exogenous 0.1 mM SA solution, administrated to the wheat cariopses significantly ameliorated the negative effect of salt stress in first
week of germination in laboratory conditions. -
Environmental friendly irrigation of vegetables with high salt content water
115-119Views:149Horticultural activities have been characteristic in the hobby gardens located in the northern and western areas around the town of Karcag for more than 300 years. During the droughty summer periods, characteristic to the region, the water demand of vegetables and fruit trees are covered by irrigation, for that the water is gained from drilled wells. These well waters contain high amount of dissolved salts contributing to the secondary salinization of the soil. Taking these facts into consideration an irrigation experiment was set at the lysimeter station of Karcag Research Institute. Our goal was to mitigate the risk of secondary salinization generated by irrigation with waters of high salt content and to survey the application possibilities of soil preserving irrigation.
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Effect of arginine, putrescine and spermidine on the polyamine, proline and chlorophyll content of tobacco (Nicotiana tabacum L.)
39-43Views:115Polyamines, such as spermidine (Spd) spermine (Spm) and their direct precursor, the diamine putrescine (Put) are vital and essential aliphatic amines which are also present in plants. Although ethylene and polyamines are also involved in fruit ripening, the genes coding them must also take part in other biosynthetic pathways. In the ethylene and polyamines play an important role in development of salt stress tolerance, and in responses for biotic and abiotic stresses. Exogenous application of all three main polyamines (Put, Spd, Spm) increase salt tolerance of plants, but, accordingly to previous experiments, spermidine has the main effect on the enhancement of salt tolerance. Nicotiana tabacum L. plants were grown in vitro on MS medium, the treatments were as follows: arginine (150 mg l-1), putrescine (10 mg l-1), spermidine (10
mg l-1). Proline, chlorophyll a, b and polyamine contents were measured. The obtained results show that the arginine decarboxylase and the spermidine synthase genes involved in polyamine metabolism, cannot be enhanced by exogenous addition of their precursor molecules. On the contrary, the spermine synthase gene has a positive effect to the lower-class forms of polyamines. -
Amelioration and arable land-use possibilities of Solonetz soils in the Trans-Tisza region of Hungary
107-117Views:165Salt affected soils cover about 1 million hectares in Hungary. This paper is based on the research results obtained at the Karcag-puszta long-term experimental site, where, depending on the catena, crusty (A horizon=0–7 cm), medium (A hor.=8– 20 cm) and deep (A hor.>20 cm) subtypes of the Meadow Solonetz soil could be found. The chemical reclamation was made specifically for the given site. Lime was applied on the soils with neutral or slightly acidic top layer, while on the soils with alkaline top layer, gypsum was applied. As regards internal drainage, a tube system with an average depth of 1 m and 5, 10 and 15 m drain spacing was constructed. Decreasing salt and sodium content could be measured both in drained and non-drained soils. The advantage of drainage was that the leachingout extended to the whole layer above the drain pipes, while without drainage, the Na ions exchanged from the upper layers still accumulated at a depth of 60– 100 cm. In the first two decades, the groundwater level was deeper and leaching was the dominant process. Since 2004, due to the frequently high groundwater level, the leaching and deepening of the fertile top layer has been slower.
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Usage of Different Spectral Bands in Agricultural Environmental Protection
123-126Views:80Hyper and multispectral imaging systems are widely used in agricultural and environmental protection. Remote sensing techniques are suitable for evaluating environmental protection hazarsd, as well as for agriculture resource exploration. In our research we compared aerial hyper and multispectral images, as well as multispectral digital camera images with the background data from the test site. Hyperspectral records were obtained using a new 80-channeled aerial spectrometer (Digital Airborne Imaging Spectrometer /DAIS 7915/. We have chosen two farms where intensive crop cultivation takes place, as test sites, so soil degradation and spreading of weeds can be intensive as a result of land use and irrigation. We took additional images of air and ground with a TETRACAM ADC wide band multispectral camera, which can sense blue, green and near infrared bands. We had detailed GIS database about the test site. Weed and vegetation map of the area in the spring and the summer was made in 2002. For soil salt content analysis, we gathered detailed data frome an 80x100 m area. When analyzing the images, we evaluated image reliability, and the connection between the bands and the soil type, pH and salt content, and weed mapping. In the case of hyperspectral images, our aim was to choose and analyze the appropriate band combinations. With a TETRACAM ADC camera, we made images at different times, and we calculated canopy, NDVI and SAVI indexes. Using the background data mentioned above, the aim of our study was to develop a spectral library, which can be used to analyze the environmental effects of agricultural land use.
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The effect of different genotype cattle grazing on the nutrient content of saline grasslands vegetation
46-50Views:173Maintaining saline grasslands in good condition, preserving their yield and diversity is important not only for the purpose of nature conservation, but also for farming. Therefore, the primary purpose of our study is to analyse the effect of pasture use of the smaller weight extensive and the larger weight intensive beef cattle on the grassland vegetation and nutrient content. In this way, we can answer the question whether grazing for nature conservation can be achieved with more profitable, more economical and more intense varieties.
The tests were carried out in May 2016 and May 2017, in the Hortobágy National Park (Pap-ere and Zám puszta), where a total of 16 sample areas were analysed. These areas are grazed with extensive beef cattle (Hungarian grey) and mixed genotype of intensive cattle. The associations were selected along a moisture gradient, such as wet salt marsh (Bolboschoenetum maritimi) and drier saline meadow (Beckmannion eruciformis). All the vegetative material collected both years in May was analysed for the following parameters: dry matter, crude protein, crude fibre and life-sustaining net energy content. We compared the effects of medium grazing (0.46 livestock/ha) and abandonment on vegetation and nutrient content.
We examined the effect of (i) grazing, (ii) different grasslands (salt marsh, saline meadow) and (iii) grazing of different cattle breeds (Hungarian grey, intensive beef) on the nutrient content of the vegetation of grasslands Based on our results, it was found that grazing had an impact on crude protein and life-sustaining net energy content. The highest crude protein content (12.75 m/m%) was obtained in the year 2017 in the area where higher density had been grazed for two years. For the lifesustaining net energy, the highest value (5.05 MJ/kg d.m.) was also obtained in 2017 and the lowest in 2016. Furthermore, it was found that there was no significant difference between the effect of the two cattle breeds on the parameters examined. Significant effects were observed only in the case of life-sustaining net energy: in the area of intensive beef cattle we received a higher value (5.15 MJ/kg body weight) than in the area with extensive beef cattle (4.96 MJ/kg body weight).
Our results have also shown that cattle grazing is of the utmost importance for the maintenance of both wet and mesophilous habitats. Based on our three-year study, we can say that grazing by both extensive and intensive cattle breeds is suitable for the management of saline habitats.
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Evaluation of Soil Degradation Based on High Resolution Remote Sensing Data
145-148Views:76Soil salinity is the main problem of soil degradation in the Grate Plain with cultivated area of 20% affected. Its influence is accelerated on the water managed and irrigated lands. Remote sensing can significantly contribute to detecting temporal changes of salt-related surface features. We have chosen a farm where intensive crop cultivation takes place as a test site as soil degradation can be intensive as a result of land use and irrigation. In order to evaluate soil salt content and biomass analysis, we gathered detailed data from an 100x250 m area. We analyzed the salinity property of the samples. In our research we used a TETRACAM ADC multispectral camera to take high resolution images (0,2-0,5 m) of low altitude (300-500 m). A Normalized Vegetation Index was computed from near infrared (750-950 nm) and red (620-750 nm) bands. This data was compared with the samples of investigated area. Analyzing the images, we evaluated image reliability, and the connection between the bands and the soil properties (pH, salt content). A strong correlation observed between NDVI and soil salinity (EC) makes the multispectral images suitable for construction of salinity map. A further strong correlation was determined between NDVI and yield.
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The effect of grazing of various cattle breeds on botanical composition of low-lying pasture in Hortobágy
57-63Views:161Coenological surveys were conducted in the Hortobágy National Park (Pap-ere and Zám-puszta) in May 2015 and 2016. During the tests,a total of 40 permanent plots were analyzed on grasslands grazed by extensive cattle (Hungarian Grey) and mixed genotype intensive cattle. The presence of plant species, percentages of total coverage of species and vegetation cover were recorded. Two habitat types were chosen according to their moisture content: wet salt marsh meadow (Bolboschoenetum maritimi) and drier salt meadows (Beckmannion eruciformis).
We compared the impact of increased number of animals (2016 years) and the low number of animals (2015 years, initial state) and the grazing exclusion on vegetations.
We tested: (i) what is the impact of grazing on the vegetation, (ii) how do species composition and vegetation charachteristics differ in the two habitat types (iii) and is there a difference in the impact of different cattle breeds (Hungarian gray, intensive beef cattle) grazing on the grasslands species composition? During the investigation we found, (i) that the greatest number of species was recorded in 2015, on the area that received moderate to intensive grazing (14.3 species per m2). Somewhat the number of species was reduced in 2016 due to more intensive grazing. The control group had the lowest number of species (11.7 species per m2). The undergrass and legumes cover significantly increased on intensive grazed lands. (ii) Our results indicate that the effects of different grazing differ in the two studied habitat types. On the drier grasslands greater number of species were found (16.2 species per m2), oppositely to the wet grassland (11.2 species per m2). The cover of the undergrasses was higher in the drier habitat than in the wet. (iii) The extensive beef cattle left a bigger number of species (16 species per m2) than the intensive beef cattle (11.4 species per m2). The grass cover was more intense on areas grazed by intensive cattle. The absolute and potential weeds cover showed a higher value on areas grazed by Hungarian Grey. Our two-year results suggest that grazing by both extensive and intensive cattle breeds can be a proper tool for the conservation management of alkali grasslands. -
Behavior of some Hungarian wheat varieties to seed soaking in gibberellic acid under salt stress
7-17Views:189Various abiotic stresses including high salinity strongly affect seed germination. Three Hungarian wheat landraces Gamási, Fóti and Kartali have been tested for seed germination, seedling growth and chemicals parameters when seeds were soaked in gibberellic acid (GA3) at the concentrations of 0, 75 and 150 ppm and at four levels of salinity stress 0, 5, 10 and 15 dSm-1. A laboratory experiment took place at Research Institute of Nyíregyháza. Factorial Experiment based on Randomized Complete Block Design (RCBD) in four replications has been conducted. Seed soaking in GA3 before sowing significantly affected germination characteristics, seedling properties and the results of chemical analysis. The highest germination as well as K+ content of seeds were observed at seed soaking into 150 ppm GA3. However, Na+ and proline contents in seedling were decreased. The behavior of the tested Hungarian wheat varieties was genotype-dependent. Increasing salinity stress up to 15 dSm-1 significantly affected germination characteristics, seedlings parameters and chemical analysis. Fóti variety turned out to be the best at 150 ppm GA3 compared to the other two varieties. Moreover, it had the lowest Na+ and proline contents and highest K+ content.
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Screening of paprika (Capsicum annuum L.) varieties resistant to NaCl salt stress
105-110Views:111Salinity stress is one of the environmental factors that negatively affect the growth and production of pepper plants. The 100 seeds' weight was measured. The total fresh weight of five seedlings and the growth rate of one seedling of three paprika varieties were also measured under the influence of NaCl at a salinity level threshold of (3 dS m‑1). The proportion of tissue water content in three pepper cultivars was measured according to a mathematical formula at the end of the experiment. In terms of seed weight, the (Carma) cultivar outperformed other types greatly. Except for the superiority of both (Carma, and Bobita F1) over (Fokusz) variety in total fresh seedlings weight under sodium chloride as abiotic stress, there are no significant differences in the total seedlings' dry weight and the rate of seedling growth. The non-drought-resistant type (Bobita F1) loses water the fastest, at 89.61%, compared to drought-tolerant kinds, which lose water at a slower rate (Carma, and Fokusz). The results demonstrate the (Carma) variety's numerical vigor, particularly in the growth rate. More testing is needed to determine the selection of varieties that are resistant to abiotic and biotic stresses.
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Use of extensigraph in the examination of wheat flours
17-23Views:119The extensograph is widely used in quality control and research laboratories studying wheat flour quality. The most commonly used measurements include Rm, the maximum resistance (maximum height of the curve); R5 the resistance at a constant extension of 5 cm; E, extensibility (total curve length); Rm/E, the ratio of maximum resistance to extension; and A, the area under curve. The extensograph has proved useful in the classification and assessment of flours on the physical dough properties. Another important application is its use in wheat breeding programs. Furthermore, extensograph is an excellent tool to study the effects of a wide range of ingredients on dough properties of wheat flour (like oxidants, enzymes, salt and additives), and it can also be used measure the changes during the frozen storage. In the present study we provide a review of the scientific literature concerning the possible applications of the extensograph.
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Testing laboratory parameters of compost tea
31-36Views:383During the industrial production of broiler chicken, a large amount of manure is produced, of which easily contained nitrogen content (without pre-treatment) is released into the atmosphere as an air pollutant. In our experiments, we aimed to prepare compost tea, also known as water extract of compost, from pre-treated poultry manure in order to create a product can be utilized as liquid nutrient supply. The poultry manure source was the Baromfi-Coop Ltd. located in Nyírjákó, Hungary, where it was treated by composting. As a result of this pre-treatment of the poultry manure, its nutrient parameters improve and nitrogen is present in a form that is better utilized for plants. Furthermore, this product is suitable for further utilization and also can be the base material for a brand-new product. For this reason the effects of compost/water ratio, incubation time, low oxygen level, and extraction time on the parameters of the resulted product were studied in the frame of developing new soil-life enhancing microbial product, so-called compost tea.
Chemical parameters of the compost used as base material strongly determined the properties of the resulted compost tea, especially the ratio of the various nitrogen forms, their concentration and the salt content. It was found that adding water at a higher rate that means 1/40 and 1/50 mixing ratios results in more cost-effective production. In the experiment the compost tea were held under oxygen-poor conditions, therefore pH of the extractions decreased, which influenced the quality and quantity of their nutrient content.
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Dilemmas of consumption habits - comparison of the quality of bottled mineral water and the public utility drinking water in Debrecen
217-221Views:102This paper tries to help to decide whether the bottled mineral water or the piped water is more advisable for consumption in Debrecen and in its regions, according to the aspects of environmental awareness and aspects of health, beside the venture of representing the functions of water in our lives and its global importance.
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Effect of sufficient and deficit irrigation with different salt inputs on the yield of cucumber
19-25Views:53Soil salinisation is considered one of the major environmental hazards threatening agricultural productivity and can be accentuated by climate change as well as the use of low-quality water in irrigation. This is the case in our study area which is affected by secondary salinisation due to the use of saline irrigation water for horticultural production. Deficit irrigation technique is implemented especially in arid and semiarid regions due to its potential to optimise water productivity while maintaining or increasing crop yield. The main objective of this study was to compare the effect of irrigation with sufficient (SD) and deficit (DD) doses. This research was carried out in Karcag in 2020. Cucumber was grown on a meadow chernozem soil and was irrigated with SD and DD of two irrigation water qualities. Soil moisture was monitored and crop yields were recorded. Despite the differences in quality and quantity of water, the application of less water by DD maintained the same yield as SD. We found a non-significant difference between the average soil moisture contents under the treatments (15.5 v/v% for SD and 13.5 v/v% for DD). Deficit irrigation can be an efficient technique due to its potential for improving water use efficiency, maintaining sufficient soil moisture content favourable for proper crop development and yield.
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Effect of anthocyanin-rich sour cherry extract on the level of IL-8 in LPS-induced endothelial cell
27-30Views:196The anthocyanin content of the Hungarian sour cherry is remarkable. Nutraceutical and pharmaceutical effects of the anthocyanins and their role in disease prevention have been studied extensively. Endothelial cells are involved in the pathogenesis of several inflammatory diseases. The objective of this work was to investigate pure sour cherry extract on human umbilical cord vein endothelial cells (HUVECs) as an inflammatory model. HUVECs were treated with 100 ng/mL lipopolysaccharide (LPS) and 50 mg/mL sour cherry extract or M199 medium as control. The optimal concentration range of the sour cherry extract was investigated and selected based on MTT assay measuring the conversion of the tetrazolium salt to formazan by mitochondrial dehydrogenases. The level of interleukine-8 (IL-8), a pro-inflammatory cytokine, was measured in Luminex MagPlex assay. LPS treatment significantly increased the secretion of IL-8. The pure sour cherry extract was able to attenuate this increment indicating the potent anti-inflammatory effect of pure sour cherry extract. Our results emphasize that pure sour cherry extract could reduce the LPS-induced inflammatory response thereby may improve endothelial dysfunction.
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Selenium-speciation experiments from soil samples by accept of ionchromatography-inductively coupled plasma mass spectrometry (IC-ICP-MS) method
106-111Views:129In these days, selenium is one of the most investigated microelements. It has an important proven role in many vital processes. Selenium deficiency can play a role in the contraction of many diseases (e.g. cancer, heart diseases, etc.) and in the aggravation of their clinical aspect directly or indirectly. In this paper, we study the soil samples of an outdoor experiment for the conversion of the inorganic selenium salt that was spread out in different doses. The danger of the wash-out effect was also investigated. Our experiments showed, that most of the inorganic selenite transforms to selenate form during the years, and in this selenate form it moves to the deeper layers of soil.
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Measurement of degradation on under-utilized natural turf
115-121Views:125The role of turf serving animal husbandry is significantly declining with the decreasing number of grazing livestock in Hungary. Accordingly, the area of under-utilized or non-utilized turfs is increasing. At the University of Debrecen, Institutes for Agricultural Research and Educational Farm, Karcag Research Institute we studied four types of turf utilization in three repetitions on a salt meadow with Alopecurus pratensis. As a result of the performed examinations, we identified the composition of the flora structure on the investigated area and we measured carbon-dioxide circulation and soil moisture.
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Spatially Discrete GIS Analysis of Sampling Points Based on Yield and Quality Analysis of Sugar Beet (Beta vulgaris L.)
32-37Views:74Fulfilment of the increasing quality requirements of sugar beet production can be analysed with sampling of plants and soil at the cultivated area. Analyses of the spatial characteristics of samples require exact geodetic positioning. This is applied in practice using GPS in precision agriculture. The examinations were made in a sample area located in north-western Hungary with sugar beet test plant. According to the traditional sample taking procedure N=60 samples were taken in regular 20 x 20 m grid, where besides the plant micro and macro elements, the sugar industrial quality parameters (Equations 1-2) and the agro-chemical parameters of soils were analysed. Till now, to gain values of mean, weighted mean and standard variance values, geometric analogues used in geography were adapted, which correspond to the mean centre (Equation 3), the spatially weighted mean centre (Equation 4), the standard distance (Equation 5), and the standard distance circle values. Robust spatial statistical values provide abstractions, which can be visually estimated immediately, and applied to analyse several parameters in parallel or in time series (Figure 1). This interpretation technique considers the spatial position of each point to another individually (distance and direction), and the value of the plant and soil parameters. Mapping the sample area in GIS environment, the coordinates of the spatially weighted mean centre values of the measured plant and soil parameters correlated to the mean centre values showed a northwest direction. Exceptions were the total salt and calcium-carbonate contents, and the molybdenum concentration of the soil samples (Table 1). As a new visual analysis, the spatially weighted mean centre values of the parameters as eigenvectors were projected to the mean centre values as origin. To characterize the production yield, the raw and digested sugar contents of the sample area, the absolute rotation angles of the generated vectors were determined, which indicate numerically the inhomogenity of the area (Figure 2). The generated spatial analogues are applicable to characterise visually and quantitatively the spatial positions of sampling points and the measured parameters in a quick way. However, their disadvantage is that they do not provide information on the tightness and direction of the spatial correlation similarly to the original statistical parameters.
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Measurement of the degradation of abandoned turf
145-149Views:140With the decreasing number of grazing livestock in Hungary, the role of the turf cultivation is also significantly decreasing. The proportion of the under- and non-utilized turf is increasing. In the research conduced at the University of Debrecen, IAREF Research Institute Karcag, we studied four types of turf utilization in three replicates on a salt field with timothy grass. We determined the flora composition of the experimental area, measured the soil moisture and the carbon-dioxide content of the soil.
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Study of factors controlling the amount of 0.01 M CaCl2 extractable Norg fraction
437-449Views:181The use of new methods describing the “readily available” nutrient content of the soil is spreading on a global scale. The 0.01 M CaCl2 extractant is a dilute salt solution in which the easily soluble inorganic (nitrate-N and ammonium-N) and organic N fractions, P, K and micronutrients are also measurable. The 0.01 M CaCl2 has been tested in the University of Debrecen, Institute of Agricultural Chemistry and Soil Sciences since the 90’s. The results of the researches related to organic N fraction, performed in the last decades, and the results of the present study (originating from the long-term experiment of Karcag, 2007–2009) can be concluded as follows:The measurement of easily soluble and oxidizable organic nitrogen (Norg), besides inorganic fractions, could improve the nutrient management.The amount of the Norg fraction is determined by the soil conditions, therefore it is considered to be a site-specific parameter.Management practices and cropyear affect the amount of Norg as well. The present research confirmed that, the effect of fertilization on the amount of Norg can be explained by the changing of the yield (related to total biomass production), while the effect of cropyear is related to the differences in mineralization circumstances and yield as well.The measurement of the Norg fraction is increases the accuracy of N-supply, therefore it could prevent the environmentally harmful excess N application as well.