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The Effect of Zanthium Extracts on Germination in Relation to Interior and Exterior Factors
65-69Views:199In Hungary, the cocklebur species significantly endanger row crops (i.e. corn, sunflower, sugar beet). Their fast spreading is caused by many reasons: long-lasting emergence, reduced sensitivity to many kinds of herbicides, vigorous competitional ability, allelopathy, fast initial growth and changes in climate. The means of allelopathy is used by many species of plants in the competition with one another, as well as by the cockleburs. But the efficiency of this is influenced by many factors. Species with allelopathical effect could show different behaviour under different conditions: they can produce compounds with retarding and stimulating effects in different quantities or compositions. The actual condition of the acceptor plant and the mediator agent (i.e. soil) influence the final effect.
In this experiment influence of phenology and rainfall was studied on allelopathy of cockleburs in May, July and September. A stronger inhibition was observed on growth of cress before rain in July than in May, but this retarding effect disappeared after rainfall. However, the samples collected in September have significant inhibitory effect after rain. Inhibition of sugarbeet’s germination was stronger in May than in July, and the difference increased after rain. -
Influencing the growth kinetics of yeast strains with vitamin supplementation
113-115Views:293The aim of the current experiment was to optimize the creation process of single cell protein on plant-based substrate solution with the intention to improve end-product turn out by means of adding vitamin solution. Based on the results of the fermentation processes of yeast strains, it was concluded that the vitamin-supplementation produced its greatest effect on the dry matter production, primarily on the K. marxianus DSM 4908 strain, while it was less beneficent when it comes to the figures of wet cell mass. In addition, it can be assumed that vitamin supplementation increased the maximum specific rate of growth (μmax) and decreased the generation time (tg) significantly. In the case of the K. marxianus yeast strain on corn steep liquor treated with vitamin-supplementation, the highest (μmax) and the lowest (tg) data were observed [(0.226 h-1) and (4.4 h), respectively]. Based on the results it was found that K. marxianus DSM 4908 is expedient to be applied on corn steep liquor medium in order to determine its suitability to produce additive for feeding.
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Influence of temperature and variety on seed germination of soybean (Glycine max L. Merr) at different germination times
5-12Views:422Seed germination is an important stage in crop development that affects plant performance, crop yield, and quality. Many factors influence seed germination, and one of the most important factors is temperature. The closer the temperature is to the optimum, the faster germination will occur. Temperature affects seed germination in various plants and varies depending on the variety. Therefore, the effect of temperature on seed germination is necessary to investigate, also for soybean varieties. Soybean is one of the world's most valuable oil-seed crops. Generally, proper seedling establishment and germination of soybean seeds are crucial processes in the survival and growth cycle of the crop. Thus, a study was done to investigate the influence of temperature and variety on soybean seed germination at different times after sowing. The experiment was carried out at the Institute of Agronomy, Hungarian University of Agriculture and Life Sciences (MATE), Crop Production Laboratory. Seeds of two soybean varieties were subjected to three different temperatures: 15, 25, and 35 °C, and two soybean varieties, Martina and Johanna were tested. There were four germination times based on the days after sowing: Days 3, 5, 7, and 9. This experiment was set up with a completely randomized block design and four replications. According to the findings of this study, the Martina variety showed a better germination rate as well as higher vigor and viability than the Johanna variety in the first 12 days after sowing. At temperatures of 15 °C and 25 °C, both varieties achieved comparable percentages of viability, but they were more vigorous at 25 °C due to better total seedling length. Thus, the information gained from this study will indirectly determine and confirm the proper temperature for the initial growth of the Martina and Johanna varieties.
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Examination of the efficacy of different fungicides against Macrophomina phaseolina and Sclerotinia sclerotiorum in laboratory conditions
21-24Views:586Macrophomina phaseolina and Sclerotinia sclerotiorum are two significant fungal pathogens of sunflower. M. phaseolina causes charcoal rot and ashy stem blight in several important crop species. Sclerotinia sclerotiorum causes white mold disease which can occur as middle stalk rot, head rot and premature plant death. Due to the wide host range of the two pathogens and their survival structures, crop rotation cannot provide sufficient protection against them. In our experiment, we selected two fungicides, Mirage and Prosaro, which are widely used in practice, and we tested their efficacy against the two pathogens. The efficiency of these fungicides was tested at a concentration of 10; 20; 50; 100 and 500 ppm. The Prosaro totally inhibited the mycelial growth of both pathogens at a concentration of 50 ppm, 100 ppm and 500 ppm. The Mirage caused total mycelial growth inhibition in all treatments against both pathogens.
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The Role of Trichoderma in the Rhizosphere of Tomato Plants
67-69Views:154It is well established that microorganisms are closely associated with the roots of plants can directly influence plant growth and development. Species of Trichoderma, on the other hand are primarily studied for their ability to control plant disease. The ability of species of Trichoderma to directly promote or inhibit plant growth has been noted for many years.
Tomatoes were treated with different Trichoderma strains by seed treatment and soil inoculation. The Trichoderma were grown on malt-agar medium and conidia were washed off by sterile water for making suspension which contained 107 CFU/ml (colony forming unit/ml). The suspension was used for seed treatment and for the soil inoculation by watering as well. The artificial soil inoculation was made by Trichodermas growing on grounded maize were mixed in mould and tomato seed were sown in it. Tomato seeds were also sown in bags made of close-meshed material which allowed the soil microorganisms to colonize the roots but it simultaneously protected the roots from soil contamination. Roots were put on Trichoderma selective medium to check the root colonization of the Trichoderma.
The tomato plants were bedded out in a field in four repetition. After harvesting by hand the results supported by statistics shown that there was significant differences between the yield of the untreated and treated tomato plants by Trichoderma strains. -
Role of living bacteria and other amendment in early development of maize
53-56Views:262Different bacteria and wood ash, as a possible micro-nutrient, and liming material, was examined in our experiment on the early growth of corn seedlings.
The development of renewing energy resources includes the use of energy grasses and energy forests. The intensive land use in forestry and in agriculture may cause the acidification of soils due to the harvest, or leaching of cations. To maintain the sustainability of soils necessary to maintain it’s the buffer capacity, and pH. Beside the lime the wood ash can is one of the most effective sources to provide the sustainability of intensive land use. The soil born micro organisms play a significant role in the maintenance of soil quality. The bio fertilizer, that contains soil originated bacteria (Azotobacter, and Bacillus sp.), was used in the experiments. The plants release several organic acids by their roots lowering the soil pH, and make more available the sparingly soluble minerals. The amounts of released organic matter depend on stress intensity, as the high pH is. The soil life has a significant role to keep the soil conditions on sustainable level, since there are several similarities in nutrient uptake mechanism between the bacteria and higher plants. Advantageous effects of bio-fertilizer were observed in our experiments.
We came to the conclusion that the use of wood ash is recommended instead of lime for the improvement of acidic soils, on the evidence of its pH increasing effect. The wood ash contains several micronutrients in an optimum composition for forestry and agricultural plants. The solubility of heavy metals is very low; therefore there is no risk to use the wood ash in the agriculture and in the horticulture by our experiments. The retardation of growth at higher ash doses can be explained by the modification effect to the soil pH, as far as the original soil pH was pH 6.8, and when ash was given to the soil, the pH increases to 7.8 pH, that is unfavourable for the uptake of most nutrients.
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Correlations Between the Crown Sizes, Pruning Times and Fruit Quality of Sour Cherry Cultivars
90-94Views:271To increase the intensiveness of sour cherry production, i.e. the use of smaller trees, it is necessary to adopt growth-moderating techniques rather than using dwarfing rootstocks. Apart from the traditional technique of using rootstocks that support stronger growth, new or rarely-used techniques and methods must be adopted. Pruning should be carried out in the summer rather than in the winter, the optimal period being 1-3 weeks after harvest.
A combination of the increase in favourable exposure-time and smaller crown sizes make higher tree densities (tree/ha) and better utilisation of the crown (specific cropload) possible.
The systematic use of summer pruning leads to better quality fruit. -
Research on the allelopathic effect among the species Tanacetum vulgare and some agricultural crops
105-109Views:299The research presents the results of the allelophatic effect upon the germination and growth of plants, immediately after springing, viewed as the interaction between the species of Tanacetum vulgare sin Chrysanthemum vulgare and three other crops: wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), rape (Brassica napus ssp. oleifera L.). The experiments that were performed consisted in applying treatments with aqueous extracts obtained from the roots, leaves, stems and flowers harvested from T. vulgare plants upon the seeds of the three agricultural crops mentioned before. In all aqueous extracts, the results indicate the presence of some chemical compounds that have inhibiting allelophatic effect. The plants upon which tests were made showed great sensitivity, the results on their germination and growth being significantly negative and highly significantly negative.
The conclusions of this research sustain the idea of setting new research objectives in order to discover the chemical compounds from T. vulgare extracts that have such a stong effect and the possibilities they offer. -
Optimizing fish structure in angling ponds focusing on white fish
33-36Views:294According to new amendments of laws, commercial fishing licenses of natural waters shall not be issued from the calendar year 2016 in Hungary. However, the most settlement of white fish to angler ponds originated from natural waters. Currently, there is no sufficient quantity of fish species belonging to this group available to fulfil the settlement commitments of fish farmers. The aim of this recent study is to develop a breeding and production technology that is suitable for settlement of large quantities of this fish species of appropriate length of (4–5 cm). The following methods are investigated: aquaculture cage system (pond-in-pond system), monoculture and mixed population with different ratio of white fish in fish structure. Due to the different environmental needs of different fish species, many parameters need to be investigated, for example: stocking density, feeding, oxygen level, monoculture or mixed populations, growth potential and mortality. A preliminary experiment was carried out following the artificial propagation of ide (Leuciscus idus) following a 21-day larval rearing experiment in which the effect of different stoking density was investigated for mortality and growth. On the basis of the expected results, the optimal conditions for the aquaculture cage system will be described, regarding to the white fish stock.
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Comparison of the physiological responses of an oil-pumpkin cultivar and hybrid under different nitrogen supply conditions
65-69Views:493Cucurbita pepo var. styriaca is known as a medicinal crop among other cucurbits. The benefits of cucurbits fruits are very important in terms of human health, purification of blood, removal of constipation, digestion and supplying energy. The mutant styrian oil-pumpkin (Cucurbita pepo L. convar. Pepo var. styriaca Greb.) exposes a complete lack of lignification of the seed testa. For this reason, this kind of naked pumpkin seed is more consumable as snack than other seeds. The vegetative growth of plants is crucial for fruit production. Nitrogen is the most imperative element for the proper vegetative growth and development of plants which significantly increases and enhances yield and its quality by playing a vital role in the biochemical and physiological functions of plants.
Field studies were conducted to study the effect of nitrogen nutrition on certain physiological parameters and their differences between the styrian oil pumpkin hybrid and cultivar (Gleisdorfi Classic cultivar and GL Rustical hybrid). The small block experiment was carried out based on a factorial experiment with a completely randomised block design and four replications in the Research Institute of Nyíregyháza during the summer period of 2017 and 2018. Three different concentrations of nitrogen fertiliser was applied at sowing. Photosynthetic pigments (chlorophyll-a, -b and carotenoids) were analysed as one of the basic factor of efficient photosynthesis. The yields of two genotypes were also measured in all treatments as seed-weight. The contents of photosynthetic pigments were higher in the oil-pumpkin hybrid, mainly the carotenoids in 2018. The vegetation period of 2018 was drier than in 2017, the hybrid oil-pupmkin was more efficient in stress tolerance than the cultivar with higher carotenoids pool. The obtained yield was more pronounced in the oil-pumpkin hybrid than the cultivar in all examined years. The hybrid GL Rustical was more sensitive to nitrogen treatment than the cultivar Gleisdorfi Classic.
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The effect of wet compost extract on the root tubers of green pea
51-54Views:307Nitrogen plays significant role in the life of plants, it could be the main limiting factor of plant growth. Sustainable plant nutrition pays attention to satisfy the plants’ nutrient demand without chemical fertilizers, e.g. by bounding the atmospheric nitrogen. The nitrogen fixing organizations play important role in supplying plants with nitrogenbecause the N2-fixingbacteria can fix high amounts of nitrogen.
Many effects of the sewage sludge compost extracts is known in the literature. We studied the effect of sewage sludge compost water extract in laboratory conditions on the growth of Rhizobium spp. isolated from green pea, while in a small plot experiment thepea-Rhizobium symbiosis were studied on sandy soil in the Nyírség region. The extract was produced under aerobic conditions. The compost extract was applied before and/or after sowing. In the laboratory experiments we used the sterile version of extract, in different doses.
In our work we present the effect of compost water extract on the number of green pea roots nodules, dry weight of the plant and reproduction of the Rhizobium bacteria. -
The effect of dissolved oxygen on common carp (Cyprinus carpio) and basil (Ocimum basilicum) in the aquaponics system
89-96Views:1075Aquaponics is an integrated system that combines fish farming (aquaculture) and hydroponic plant production. The objective of this study was to examine how the level of dissolved oxygen with or without an air pump affects water quality, fish output and plant growth parameters for common carp (Cyprinus carpio) and basil (Ocimum basilicum).
Ebb – and flood aquaponics systems (with automatic syphon) was used. Two treatments were set in this experiment, one of which was the aquaponics system without air pump (unit I), where water of the plant bed was pumped two directions, one falling back to the fish tank oxygenating the water the other was pumped to the hydroponics unit. The other system (unit II) was designed with an air pump.
In the course of the study, water quality parameters, such as oxygen saturation, dissolved oxygen (DO), electrical conductivity (EC) and nitrite were significantly different (p<0.05). Total basil biomass was higher in unit II. (5367.41 g). The final biomass of common carp were
2829.45 g ± 79.24 and 2980.6 g ± 64.13 g in unit I and unit II respectively. Weight gain (WG) and specific growth rate (SGR) showed no significant differences (p>0.05) between the treatments. -
Correlations of the growth indexes and yield of winter wheat in a long-term experiment
139-144Views:251The experiments were carried out at the Látókép experimental station of the Centre for Agricultural Sciences of University of Debrecen on chernozem soil in a long term winter wheat experiment. As forecrop rotation, we set up two models: a biculture (wheat and corn) and a triculture (pea, wheat and corn). We applied three levels of nutrients during the fertilization process (control, N50P35K40 and N150P105K120). The third variable studied was irrigation in case of which we tested non-irrigated variables (Ö1) and irrigation variables complemented up to the optimum (Ö3).
The effect of pre-crops, irrigation and nutrient-supply levels on some growth-parameters (LAI, LAD), weight of dry matter, just as SPADvalues and yield amounts of winter wheat has been investigated in this experiment. We tried to find out the extent of relationship between the different parameters, and we used the correlation analysis. The correlation analyses have confirmed that all of the investigated parameters had almost in all cases close positive correlation to the yield amount. These results have confirmed that the leaf area, the leaf duration, the SPADvalues, the fertilization and the forecrop have altogether resulted in the production of maximum grain yields.
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Regional innovation: State of the art in the Észak-alföld region
139-143Views:176Innovation and innovation performance is gaining increasing importance in European policy, as the targets determined by the Lisbon priorities related to innovation seem to be still far away from the reality in some European countries and at EU level as well. Although the economic crisis determined the priorities of economic development and made it more complicated to dedicate more budget of Research and Development, one of the five ambitious objectives of the EU’s growth strategy for the coming decade: Europe 2020: A strategy for smart, sustainable and inclusive growth is still focusing on innovation. As the Lisbon Strategy determined the paths for national targets in the given areas, Europe 2020 will be adopted as well by the Member States.
In this context, the current work would like to give a short overview how Lisbon Strategy effected the national and regional innovation system in Hungary and will analyze its impact on Észak-Alföld’s region Research, Development and Innovation performance in the recent years and the transition of regional innovation governance system. -
Productivity of thinned black locust (Robinia pseudoacacia L.) stands in Hungary: case studies
181-186Views:339Black locust (Robinia pseudoacacia L.) is one of the most important introduced tree species in Hungary, covering more than 24% of the stocked area and providing approximately 20% of the country’s annual timber cut. Consequently, the research and development activities related to the improvement of the growing technology of black locust are also important, especially in mitigating the negative effects of climate change. The aim of this study was to quantify growth and yield, responses of even-aged 12–31–year–old black locust stand to thinning in Hungary. The study has proved that irrespective of the yield class, age and thinning intensity, thinning could not increase effectively the cumulative volume production. As percentage of the control it has changed between +0.6 and -8.4%. On the other hand, it could increase the stand value based on stem quality index by 10–21%.
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Data to the growth of yoy (0+) tenches (Tinca tinca L.) in Tisza Lake
102-105Views:220Young of the year (YOY) tenches (Tinca tinca L.) were caught in relatively great number in Tisza-Lake at the Tiszafüred section on last days of October in 2004. The standard length of these fishes varied between 20 and 46 mm. It seemed that the collected sample could provide appropriate data to examine the size-structure of YOY tenches, so we measured their standard body-lengths in mm and -weights in hundred parts of gramm unit. After the statistical analysis were found the follows:
1. The curve of length frequency corresponds with normal distribution, i.e. there was not found multimodal curve as could be expected in the case of a periodic spawning species.
2. The average standard length of the examined individuals – although the environmental conditions of the population is good – was only 31 mm. It is far below the 37 mm average of the lowest growth of tench population was found in the scientificpapers.
3. More than 20% of measured sample belonged to the range of 20-26 mm. The estimated ages of these fish specimens are about 70 days, according the scientificpapers.
By right of above mentioned can be concluded that the sample represents only one age-group from a late spawning, and not the whole range of YOY tenches, so these data can give information about the size structure only of this group. The 31 mm average standard length of individuals, origin from spawning in late July or beginning of August is accordance with age and environmental conditions as well. -
Essential oil extraction from herbs and their use in the food industry
59-74Views:589Essential oil extraction of wild caraway and thyme was performed using a classical (HD) and microwave hydro-distillation (MWHD) and a laboratory supercritical fluid extraction (SFE) with a carbon dioxide as solvent. Our experiments demonstrated that the extraction yield of the essential oil performed in same conditions was influenced by the location of growth area; the maximum extraction yield of 10 ml 100 g-1 caraway was obtained from dried seeds collected from Csíkmadaras. This quantity far exceeded the yield of the Újtusnád samples. In the case of wild caraway (Carum carvi L.), the extraction method influenced thecomposition of the essential oil (carvone/limonene ratio), the highest limonene content being achieved by classical hydro-distillation. In the case of thyme, this effect was not detected, the thymol/carvacrol ratio was independent from the given extraction method. The obtained thyme essential oil possesses high antimicrobial activity demonstrated by agar diffusion test. The thyme extract provides a good protection against microorganisms collected on the surface of fresh vegetables following bacterial stains: Citrobacter portucalensis, Pseudomonas hunanensis, Pseudomonas baetica, Pseudomonas parafulva, Bacillus mojavensis and Enterobacter cloacae. Protective effect was also detected on the vegetable surface of by chitosan-based edible film coating during a 6-day-long storage period at a temperature of 4 °C. The caraway essential oil used as soft cheese seasoning with a direct, dilution-free method, proved to be unsuitable because the uneven distribution and confer a strong, unpleasant taste to the product in comparison with the ground wild caraway seed-dressed cheese.
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Unraveling changes in the duck microbiome and inflammatory processes due to allithiamine-enriched feed
163-171Views:565The gastrointestinal tract of poultry harbors a diverse and intricate microbiome that plays a crucial role in nutrient digestion and absorption, immune system development, and enhances resistance against pathogens. Maintaining a healthy state and proper production is fundamentally determined by the symbiosis between the host and microbes. Due to genetic and technological improvements, intensive growth rate can be associated with many pathological conditions, such as increased susceptibility to infections. Intestinal inflammation in poultry industries detrimentally affects productivity by hindering nutrient absorption and the efficient allocation of nutrients for growth. The host releases different biomarkers in response to inflammation. Hence, there is an utmost interest of reliable, precise, sensitive and robust biomarkers to evaluate both the gastrointestinal health status and inflammation in poultry. The aim of this study was to determine how the developed feed prototype (allithiamine) affects the community diversity in raised duck, and the relationship between gut microbiome composition and inflammatory factor as calprotectin, using targeted 16S rRNA gene amplicon sequencing and Chicken Calprotectin ELISA Kit.
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The effect of drought and cropping system on the yield and yield components of maize (Zea mays L.)
51-53Views:296Different Cropping Systems have many advantages and ensure better crop growth and yielding. Its combination with other agronomic measures can ensure optimal crop density for maximum crop growth and photosynthesis efficiency. The aim of this study was to investigate the influence of different cropping systems on monoculture and biculture rotations [maize- wheat]. The study found that crop rotation does not have a significant effect on the grain nutrition quality, Leaf Area Index (LAI) and Normalized Difference Vegetative Index (NDVI) but has a significant effect on the Soil-Plant Analysis Development (SPAD). Yield and yield components were significantly influenced by crop rotation in this study as yield, plant height, cob weight and number of grains per row all recorded lower mean at 5% probability levels.
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The Role and Significance of Soil Analyses in Plant Nutrition and Environmental Protection
3-8Views:269Hungary has a rich history of soil analyses and soil mapping. Our main tasks today are the preservation of soil fertility as well as balancing the goals of production and environmental protection. The main requirement of agricultural production is to adapt to ecological and economic conditions.
In a series of consultative meetings in the past seven years, representatives from Central and Eastern Europe have analyzed nutrient management practices in their respective countries. According to a joint memorandum agreed upon in 2000, in the countries awaiting accession, the quantity of nutrients used per hectare is considerably smaller than the Western-European usage targeted through special subsidies. The current low nutrient usage contradicts the principles of sustainability and that of the efficient use of resources, jeopardizing soil fertility.
In Hungary, the use of inorganic fertilizers underwent a dynamic development, which manifested itself in an almost tenfold usage growth between 1960 and 1985. This growth slowed down somewhat between 1985 and 1990 and then reduced dramatically after 1990, reaching record lows at the usage levels of the 60s. The nutrient supply has had a negative balance for the last 15 years.
The increasing and then decreasing usage trends can equally be detected in the domestic yield averages of wheat and corn as well as in the nutrient supply of soils. Yields were the largest when usage levels were the highest, and decreased thereafter. Draughts have also contributed to smaller yields. The dramatic decrease in the use of inorganic fertilizers when adequate organic fertilizers are lacking endangers our soils’ fertility.
About 50% of soils in Hungary are acidic. Acidity is mostly determined by soil formation, but especially on soils with a low buffering capacity, this acidity may intensify due to inorganic fertilizers. Sustainable agriculture requires the chemical improvement of acidic soils. According to their y1 values, the majority of our acidic soils need to be improved. This chemical soil remediation is required in 15% of the acidic soils, while it’s recommended for another 20% of these soils.
Results of the analyses conducted in the framework of the soil-monitoring system set up in Hungary in 1992 show that in 95% of the analyzed samples, the toxic element content is below the allowable limit. Cultivated areas are not contaminated; toxicity above the legal level was found only in specific high-risk sampling areas: in the vicinity of industry, due to local overload. The basic principle of sustainable agriculture is to preserve soil fertility without undue strain on the environment. The intensity of the production needs to be considered according to the conditions of the site; i.e.; nutrient management needs to be site-specific. It is recommended to differentiate three types of cultivated land in terms of environmental sensitivity: areas with favorable conditions, endangered areas, and protected areas, and then to adopt nutrient management practices accordingly. To meet all the above-mentioned goals is impossible without systematic soil analysis. Tests conducted by the national monitoring system cannot replace regular field measurements. -
Yield table for selected black locust (Robinia pseudoacacia L.) cultivars
193-198Views:497In Hungary, the black locust (Robinia pseudoacacia L.) can be considered as the most important fast-growing, stand-forming introduced tree species. Due to its positive growing technological characteristics as well as wood utilization possibilities, at the present, black locust is the most widely planted tree species in Hungary, covering 25% of the country’s total forest area. One of the important tasks ahead of Hungarian black locust growers is to improve the quality of black locust stands with introducing selected cultivars. For the estimation of the growth rate and yield a numerical yield table has been constructed on the basis of surveys of the experimental plots established in pure,managed ’Nyirségi’ ,’Üllői’ and ’Jászkiséri’ black locust cultivars’ plantations which can be suitable for sawlogs production. In the course of 56 stand surveys the key stand characteristics were measured, and then, were reconsidered the average height, diameter (DBH), volume, basal area and stem number given separately for the main (remaining), secondary (removal) and total stands per hectare. The programmable editing procedure allows to extention and formal change of information content of the yield table according to different demands.
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Characterisation of a thermotolerant yeast, Kluyveromyces marxianus CBS712
7-13Views:397Fermentation at high temperature with application of thermotolerant microorganisms is a technological advantage in bioethanol production. Among the yeasts, K. marxianus has outstanding thermotolarance. The industrial application of the IMB3 strain occurs usually at 45C. The final aim of our project is the genetic modification of the K. marxianus CBS712 strain in order to achieve ethanol production at higher temperature than the currently applied. This requires the characterization of the CBS712 strain, with special attention to the determination of the temperature limit of its growth and the amount of the ethanol produced. The temperature limit of growth was 48C in YPD medium. Elevation of the temperature above 45C led to an exponential drop of the cell viability. Ethanol production was tested in shaking flasks, in MYFM medium, under oxigene limited conditions, applying variable concentrations of glucose (12–20%) and different temperatures (45–47 ºC). Preliminary results have revealed that the elevation of glucose concentration increased the amount of ethanol produced. The amount of ethanol (appr. 5%)+ produced at the highest glucose concentration was not different at the tested temperatures (45, 46 and 47 ºC). The observation indicates the potential in raising the thermotolerance of the strain.
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Examining reaction of sunflower genotypes on planting time on chernozem soil
93-99Views:256We studied the effect of planting time on plant pathological factors, leaf area index and yield production by applying various fungicid treatments on two different sunflower genotypes in 2013.
By delaying planting time, both the extent of Diaporthe, Alternaria and Phoma infections decreased. The differences between the volume of infections were significant in the case of the early and late sowing time results. The application of fungicide treatments induced a notable decrease in the extent of infections for all three pathogens examined. The LAI-values varied between 0.3 and 5.6 m2/m2 in 2013 depending on the hybrid, sowing time and treatment. Stocks planted at distinct times reached maximum leaf coverage at different times. The planting time and the fungicide treatment had a significant effect on the formation of the leaf area. In the case of average and late planting times, fungicide treatments elongated the preservation of the green leaf area.
With respect to the yield amount, average planting time (27 April, 2013) turned out to be optimal in 2013 (control – NK Ferti: 4.621 kg ha-1, PR64H42: 4.196 kg ha-1; double-treated: NK Ferti: 5.282 kg ha-1, PR64H42: 5.090 kg ha-1). Fungicide treatments resulted in significant yield growth in all cases during our research.
We applied Person correlation analysis to evaluate the hybrids’ sensibility to infections and our results varied in the case of Diaporthe and Phoma (r=-0.343*, -0.379**). Infections of the three pathogens were significantly reduced by delaying the planting time and applying fungicides. Late sown stocks preserved the green leaf area for a longer period. Besides, the application of the fungicide treatment and the hybrid itself also led to the preservation of the green leaf area. However, pathogens examined notably decreased the leaf area by the end of the growing year. The fungicide treatment had a remarkable effect on yield growth (r=0.603**). Furthermore, the presence of higher LAI-values in the period prior to August also induced higher yields.
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The effect of sulphur and nitrogen supply on the growth and nutrient content of spring wheat (Triticum aestivum L.)
65-70Views:447Sulphur is an essential element for plants. Decreasing sulphur deposition from the air, and the use of more concentrated phosphate fertilizers, which contain no sulphur, has led to reports of sulphur deficiencies for wheat. Sulphur deficiency significantly affects yield and also the quality of wheat. The pot experiment was set up on calcareous chernozem soil at Látókép, Hungary, test plant was spring wheat (Triticum aestivum L). Seven treatments were used where nitrogen and sulphur were supplied as soil fertilizers in increasing rates (NS1, NS2, NS3) and in foliar fertilizer as well (NS1+fol., NS2+fol., NS3+fol.). Plant aboveground biomass production was determined in samples taken in the stages of development BBCH 29-30, 51-59, 61-69, 89. The nitrogen and sulphur content of straw and grain were measured. N/S ratios of grain and straw were calculated. The weights of grain were ranging between 8.6–16.1 g/pot. NS2 and NS2+fol. treatments produced the highest values. Foliar fertilizer had no further effect on grain. Analysing the values of the straw, it was observed that tendencies were similar to values of grain. The NS2 treatment produced the highest weight of straw and the NS3 rate already decreased that amount. The obtained results show the unfavourable effect of excessively high rate applied in NS3 treatment. The supplementary foliar fertilizer had no significant influence on the weight of straw. Both N and S-uptake of plant was very intensive at the stem elongation stage, then the N and S-content of plant continuously decreased in time in all treatments. The N-content of grain ranged between 2.215–2.838%.The N-content of grain slightly increased with increasing of nitrogen doses. In the higher doses (NS2, NS3) foliar fertilization slightly increased the nitrogen content of grain, although this effect was not statistically proved. The N-content of straw varied from 0.361 to 0.605%. The growing dose of soil fertilizer also considerably increased the nitrogen content of straw. Foliar fertilization further increased the nitrogen content of straw. The S-content of grain ranged between 0.174–0.266%. The lowest fertilizer dose (NS1) significantly increased the sulphur content of grain. The further increasing fertilizer doses (NS2, NS3) did not cause additional enhance in sulphur content of grain.The foliar fertilizer also did not change the sulphur value of plant. The increasing amount of soil fertilizer and the supplementary foliar fertilizer had no effect on the sulphur content of straw. The treatments influenced the N/S ratios of grain and straw. On the basis of experimental results it can be concluded that the examined nitrogen and sulphur containing soil fertilizer had positive effect on the growth and yield of spring wheat grown on the calcareous chernozem soil. The soil fertilizer application enhanced the grain nitrogen and sulphur content. The highest rate of fertilizer (600 kg ha-1) proved to have decreasing effect on the yield. The sulphur and nitrogen containing foliar fertilizer did not have significant effect on the yield parameters but slightly increased the nitrogen content of plant. -
Exogenous salicylic acid treatments enhance tolerance to salinity of wheat (Triticum aestivum) plantlets
34-38Views:235Salt stress, an abiotic stress, determines modifications of some biochemical indicators, like, antioxidant enzymes, proline (amino acid
accumulate in higher plants under salinity stress) content, and some physiological processes including: plant growth and development. In
this paper we studied the influence of exogenous treatment of wheat seeds, with 0.1 mM salicylic acid (SA) solution, in the plant response to
salt stress. The treatment was applied by presoaking the seeds in the treatment solution for 12 hours before germination. The results showed
that exogenous 0.1 mM SA solution, administrated to the wheat cariopses significantly ameliorated the negative effect of salt stress in first
week of germination in laboratory conditions.