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Climatic water balance in Hamelmalo, Eritrea
69-76Views:466Agricultural production is an important sector for peoples to live, but it is highly affected by climate change. To have a good production we need to understand the climatic parameters which adversely affect production. Hamelmalo, which is located in the semi-arid area of Eritrea, is vulnerable to climate change and this is realised in the total production loss. Nevertheless, there is no concrete reference about the climate of the region due to lack of data for a long time. Changes in precipitation (P), evapotranspiration (ET) and, implicitly, in the climatic water balance (CWB), are imminent effects of climate change. However, changes in the CWB, as a response to changes in P and ET, have not yet been analysed thoroughly enough in many parts of the world, including Eritrea. This study also explores the changes of the CWB in the Hamelmalo region, based on a wide range of climatic data (P, relative air humidity and evaporation pan necessary for computing potential evapotranspiration (PET) with the pan evaporation method) recorded at Hamelmalo from 2015-2019. This analysis shows that the annual cumulative CWB for Hamelmalo is negative in 67% of the years. The dry season without precipitation leads to negative CWB and the change in CWB only starts from the raining or crop season. Based on this recent study, 2015 had the highest PET and lowest P, and this resulted in the lowest CWB in the investigated period. Opposite to this, 2019 had lower PET and highest P, which led to the highest CWB. However, the monthly values of CWB did not correlate with the annual P or ET. On the base of our study, it can be concluded that PET and P were very variable in the investigated years and P was the most influential elements of CWB.
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Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:809The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.
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Yield and sward composition responses of a native grassland to compost application
35-38Views:368A major part of the animal products are based on the grasslands, due to the fact that the grassland ecosystems can be found all over the globe. In places where economical and successful crop production cannot be realized, the grassland based animal husbandry can be an efficient way of food production. In addition these ecosystems have an important role in carbon sequestration, and with their rich flora – and the fauna connected to it – in conservation of biodiversity. The protection of nature, and the sustainable agriculture is getting more and more attention in the European Union, but looking at the consumers’ needs, the production of healthy food cannot be neglected either. Because of these facts, the effects of two specific composts - which are officially authorized in organic farming, in Agri-environment Schemes and Natura 2000 programs – on grass yields and sward compositions were investigated in a field trial. The investigation took place in Hungary, on a natural grassland based on solonetz soil. The first type of compost was a natural one (N) without any additional material and the other one was enriched in phosphorus (E). Both was produced by the research institute, made of sheep manure. Three rates of compost (10 t ha-1, 20 t ha-1,30 t ha-1) were tested on 3 m×10 m experimental plots. Every treatments had four replications and both type of compost had four-four control plots too, this way 32 experimental plots were included in the investigations. The yield of the pasture was harvested two-times (in May and in September) and before cutting the plots measurements on botanical compositions were made. Samples for laboratory analysis were also taken. Dry matter yield and crude protein content was measured in laboratory and with the received data the yield per unit area was calculated. Based on the research results we can say that the application of compost in any dose inflicts higher dry material and crude protein yield. The changes were partly due to some positive changes in sward composition, because of the better nutrient conditions. The research results indicate, that use of organic compost can be an efficient way to increase grass yields in a sustainable way.
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Impact of nitrogen and sulphur fertilization on the growth and micronutrient content of spring wheat (Triticum aestivum L.)
211-219Views:385Micronutrients are as important as macronutrients for crops. Each micronutrient has its own function in plant growth. Zinc is important for membrane integrity and phytochrome activities. Copper is an essential micronutrient required for the growth of wheat. Manganese is required for enzyme activation, in electron transport, and in disease resistance. The pot experiment was set up in greenhouse on calcareous chernozem soil Debrecen-Látókép with a spring wheat. In certain development stages (according to BBCH growth scale of wheat), at the beginning of stem elongation (29–30), at the heading (51–59), at the flowering (61–69) stage three average plants were removed from all pots for analysis. Fresh and dry weight of the plant samples were measured. Plant leaves after drying were digested by HNO3-H2O2 methods and manganese, zinc and copper contents of plant were quantified by atomic absorption spectrophotometry. At the flowering stage, when the nutrient uptake of plants is the most intensive, the weight of wheat ranged between 0.94–1.57 g plant-1. In this development stage, the NS2 treatment produced the highest weight of wheat, and compared to this the NS3 treatment decreased that value already. The results show unfavourable effect of NS3 treatment. On the basis of microelement content of wheat and the weight of a plant, nutrient uptake by plant were calculated. At the beginning of growth the starter treatment had positive effect on Cu-uptake compared to the NS1 treatment, where the same dose of fertilizer was stirred into the soil. Wheat is very sensitive to copper deficiency, so copper dissolved by starter treatment could be favourable to the early development of wheat. At flowering stage the Zn-uptake of wheat became the highest and it was between 133.7–234.6 mg plant-1. The Mn-uptake of wheat plant was higher than the Cu- and Zn-uptake of wheat.
This phenomenon can be explained by the fact that the untreated soil had higher Mn-content, than Cu- and Zn-content. To summarize the results, it can be stated, that the copper uptake of wheat was more affected by the different treatments in the stage of stem elongation, while Mn- and Zn-uptake of wheat were influenced primarily in the stage of heading and flowering.
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A review on fish in vitro digestion studies
55-63Views:158(1) Background: In vitro experiments in fish have been developed to search for dietary substitutes of fish meal in aquafeeds by measuring the digestibility of various feedstuffs by simply simulating the conditions of the stomach or portions of the digestive tract. In vitro digestion studies involving mainly commercial fish species have been conducted to determine the digestibility of conventional and alternative ingredients in fish diets using diverse digestion models. However, there remains a significant knowledge gap, particularly regarding the enzyme functionality, digestion mechanisms in many fish species, and the factors influencing enzymatic activity. This review article is focused on the importance of the use of different enzyme sources in the in vitro digestion model to predict protein hydrolysis.;(2) Methods: For this review, a comprehensive analysis of articles was conducted to gain insights into diverse enzyme interactions and feed evaluation assessments, from the nutritional science and biotechnology sector.; (3) Results: A total of 36 peer-review papers, which include original, and review articles were selected.; (4) Conclusions: In vitro digestion studies offer a valuable tool for evaluating the digestibility of conventional and alternative feed ingredients, helping to identify sustainable dietary substitutes for fish meal while reducing dependence on live animal trials.
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Growth and yield patterns of black locust (Robinia pseudoacacia L.) sample trees affected by site conditions: case studies
125-128Views:334The trees removed from the long-term experiment plots are available for the measurements as lying trees. Through the determination of the volume in sections along the stem, the stem form, the stem volume and other factors can be specified. The comparison of the stems of individual trees of first and third yield classes of black locust (Robinia pseudoacacia L.) stands shows that site conditions have a main effect on the yield (mean tree volume). The difference can be as high as 53% at the age of 30 depending on the sites. To determine the growth patterns based on tree volume is rather a new approach in the light of the relevant literature. The obtained results also highlight the importance of choosing the appropriate tree species for a given site.
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The scientific background of competitive maize production
33-46Views:1061The effect and interaction of crop production factors on maize yield has been examined for nearly 40 years at the Látókép Experiment Site of the University of Debrecen in a long-term field experiment that is unique and acknowledged in Europe. The research aim is to evaluate the effect of fertilisation, tillage, genotype, sowing, plant density, crop protection and irrigation. The analysis of the database of the examined period makes it possible to evaluate maize yield, as well as the effect of crop production factors and crop year, as well as the interaction between these factors.
Based on the different tillage methods, it can be concluded that autumn ploughing provides the highest yield, but its effect significantly differed in irrigated and non-irrigated treatments. The periodical application of strip tillage is justified in areas with favourable soil conditions and free from compated layers (e.g. strip – strip – ploughing – loosening). Under conditions prone to drought, but especially in several consecutive years, a plant density of 70–80 thousand crops per hectare should be used in the case of favourable precipitation supply, but 60 thousand crops per hectare should not be exceeded in dry crop years. The yield increasing effect of fertilisation is significant both under non-irrigated and irrigated conditions, but it is much more moderate in the non-irrigated treatment.
Selecting the optimum sowing date is of key importance from the aspect of maize yield, especially in dry crop years. Irrigation is not enough in itself without intensive nutrient management, since it may lead to yield decrease.
The results of research, development and innovation, which are based on the performed long-term field experiment, contribute to the production technological methods which provide an opportunity to use sowing seeds, fertilisers and pesticides in a regionally tailored and differentiated way, adapted to the specific needs of the given plot, as well as to plan each operation and to implement precision maize production.
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Doing sport is good – Responsible for popularization of sport
71-75Views:339Responsible leaders of more and more companies build legal, ethic, moral, environmental and social aspects in their goals and values beyond profit maximization. The number of companies whose activities in terms of social responsibility (CSR) has clear connections with promoting and putting forward sports shows an increasing tendency.
The aim of this study is the conceptual clarification of corporate social responsibility, and emphasize the CSR’s significance in sport management, next the analysis of 3 top food and beverage companies’ (Coca-Cola, Nestle, Danone) CSR activity in special regard to sport management in Hungary and abroad.
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Comparative study of a winter wheat variety and hybrid sown after different pre-crops on chernozem soil
63-69Views:501Wheat production is a determining branch within Hungarian crop production (produced on nearly one million hectares). Weather anomalies caused by climatic change confirmed the importance of the biological background (variety, hybrid) in wheat production. The adapting ability and reaction of different wheat genotypes towards nutrient supply were studied in a long-term field experiment on chernozem soil type in the case of different pre-crops (sunflower and maize). According to the experimental results of the vegetation of 2017/2018, the yield of the variety Ingenio sown after the sunflower as previous crop ranged between 4168 and 8734 kg ha-1, while in the case of maize as previous crop, this value ranged between 2084 and 7782kg ha-1, depending on the applied nutrient supply level. The studied genotypes produced rather significant yield surplus as a response to the application of mineral fertilization (4.6–5.1 t ha-1 after sunflower and 5.7–6.3 t ha-1 after maize). Optimal mineral fertilizer dosage was determined by both the genotype and the pre-crop. N-optimum values of wheat genotypes was determined using regression analysis. In the case of the variety Ingenio sown after sunflower, the optimum range was N144-150+PK, while after maize, it was
N123-150+PK, respectively. For the hybrid Hyland, these optimum ranges were N114-120+PK, just as N150-153+PK, resp. The application of optimal mineral fertilizer dosages improved water utilization of the studied wheat genotypes to a significant extent. WUE values of the control, unfertilized treatments ranged between 4.1–8.3 kg mm-1, while in optimal fertilizer treatment, it ranged between 15.5 and 17.4 kg mm-1. -
Analysis of the main parameters of spring and summer food compound of roe deer (Capreolus capreolus L.) on the two hunting grounds of the Alföld plain
7-10Views:252It is widespread that roe deer are very choosy. It needs this sorting because the micro organisms, which help the digestion of high fibre plants, are missing in its stomach that is why they are mostly called „concentrate selectors” (Hoffmann, 1985, 1988, 1989).
These animals should mostly eat easily digestible plants with high nutrition level (pulses, buds, sprouts and flowers), and they are able to do this sorting because of their small mouth size. In winter there is a lack of these plants, so the high selectivity occurs only when the feed is in abundance.
Examining the amount and quality of vegetation available on the habitat of roe deer we can identify the species which can satisfy their feed demand. It is known, that roe deer as other large ruminants, from the plant abundance prefer certain plants and plant parts while there are others which are avoided. The identification of the eaten species and the rate of their occurrence in the feed is the first step to become acquainted with the
interaction between animal and the surroundings. -
Results of weed surveys in greening plants
53-57Views:325Greening crops play an essential role in Hungary's agriculture. Weeds can also cause many problems during the development of greening plants. Our research aimed to evaluate the weed control properties of greening crops sown with different germination rates. Analysis of the effect of crop rotations on weed density. Comparison of weed growth in control, fertilised and greened areas. In October of 2021, a weed survey was carried out in lupin (Lupinus albus L.), common vetch (Vicia sativa L.), oil radish (Raphanus sativus var. oleiferus L.) and buckwheat (Fagopyrum eculentum Moench). During the weed survey, we determined the different weed species and their abundance. In terms of seed rates, the higher seed rates for lupin, oil radish, and buckwheat may be worth choosing for weed suppression. Plots in rotation III had the lowest weed incidence of all greening crops. The probable reason for this finding is that there was no prior greening in rotation III. For greening, the choice of buckwheat and oil radish will result in higher weed pressure. The most important weeds were the cereals sown before the greening crop. Fertilised plots had minimally fewer weeds than control plots. Research results show the difficulties of weed control in herbicide-free greening crops.
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‘Kindergarten’ keeping-system in farrowing house: effect the socialization of piglets on weight performances, fecal cortisol metabolite level and post-weaning behavior
167-174Views:451This study aimed to examine whether litters’ let-together system before weaning (‘kindergarten-system’) has any stress effect and effect on post-weaning behavior, respectively. One week before weaning piglets were socialized by removing four adjacent farrowing crate walls. After weaning, piglets from the same experimental groups were housed in the same rearing crates. The piglet’s body weight was measured at birth than weekly till the 7th life-week. Fecal cortisol metabolites were measured for evaluation of the adrenocortical activity. Personal observation and evaluation were carried out for behavioral analyses after weaning. There were no significant differences in weight development and cortisol metabolite levels between the control and experimental groups. Behavioral analysis showed that pigs grow up in a socialized system, rapidly evolve ranking in the rearing crates, and the self-maintenance and social behavior were more pronounced. In control groups, the activity involving movement (exploring, walking, and running) was much more decisive. Recent work suggested the beneficial effect of the ‘kindergarten’ system in the farrowing house in animal welfare aspects.
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Comparative analysis of physiologically important fatty acids
71-76Views:488Fatty acid composition of some vegetable oils, like wheat germ, walnut, peanut, hempseed, linseed, sunflower-seed, olive, rapeseed, grape seed or pumpkin seed, analysed at Food Science Institute of Debrecen University, are summarised here. The effect of heat treatments usually used in Hungarian cuisine was examined in this paper.The influence of different fatty acids on human health is also reviewed.
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Toxic elemental content of Hungarian blaufränkisch wines
87-90Views:580Toxic elemental contents are one of the food safety risks in wines. Therefore International Organization of Vine and Wine (O.I.V.) defined the limit of some elements in it. Thirty Hungarian blaufränkisch wines were analysed by ICP-MS in order to determine the concentration of toxic elements. All wines are passed by the O.I.V limits to toxic element content (Cu, Zn, Cd, Pb) according to analysis. Copper is the only outlier in some samples, but they also did not overstep the limit. In the case of zinc the maximum is approximately fourth, in case of cadmium and lead the highest concentration is less than tenth and sixth of the O.I.V. limit, respectively.
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Effects of the development of production factors on productivity
13-18Views:253In every economic sector, the enacted and expected quality of the products strongly affects its market entry and subsistence. The aim of management is success, competitiveness and subsistence that require adequate human and material resources and enough production stock. We must also notice that the quality of tools and instruments significantly influences work productivity. This applies to both plant production and animal husbandry.
The author researched milk production factors with methodical observation, document analysis and interviews. Data were collected with a ten-year interval, thus this assay can highlight the field, volume and sources of investments during this period and that the production stock increased in most farms and decreased in few. The data also shows the influence of investments on work productivity - there are differences between farms of equal size, so there are still ways of development.
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Renewable energy on animal farms: support system and practical application
13-17Views:263Renewable energy applications were examined in animal farms. The investment support system for animal farms – which has renewable elements – has been summarized through analyzing the legislation and future prospects. The most important in this topic is the Animal Farm Modernization Support, which had several application periods during the 2007–2013 CAP budgets. For analyzing practical application of renewable energies interviews and farm visits have done in Hajdú-Bihar County. The questions covered the general attributes of the farms and the details of the (existing or planned) renewable energy application. In terms of economic analysis saving, the investment return time and the employment effect were examined. The results show wide variety of applied renewable energy application. The investment supports plays great role in renewable energy projects by lowering the return time; and through additional points in application assessing, which is an incentive for farmers to include renewable energy element in their modernization projects.
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The analysis of flour blends as affected by the behaviour of two different quality flours of triticale under different fertilizer treatments
5-8Views:547Triticale is likely used in many countries in human consumption, due to its advantageous agronomical and nutritional properties mostly in blends. The baking quality of blends depends not just on the proportions of the used flours but also on their individual quality what can be influenced by fertilizer treatments.
22 flour blends were prepared with commercial wheat flour and triticale wholemeal flour in proportions from 0% to 100%. The triticale was treated with different amount of fertilizers (N30P30K30, N60P0K0). Changes of wet gluten contents and extensograph parameters of the blends were determined. The quality of blends significantly depends on the fertilizer treatment of triticale beside the proportions of the flours. When the N60P0K0 treated triticale was used in blends, wet gluten and extensibility values were significantly higher, but in case of resistant to extensions (Rmax) the N30P30K30 treated samples gave higher values. The measured values of wet gluten and extensibility were above the expected values (synergism), while in the case of resistance to extension the expected values were higher than the measured values (antagonism).
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Timing it right: The Measurement and Prediction of Flowering
17-22Views:147Although the model described here was developed from research in controlled environments, there is now considerable evidence that in can be applied to a very wide range of natural environments in several species. Multi-locational trials augmented by successional sowing and, if considered necessary, supplementary illumination in the field to increase daylength, can be used to estimate the values of the model coefficients: (1) to characterize germplasm collections and so predict flowering behaviour elsewhere; (2) for interpreting and understanding crop adaptation; and (3) for genetic analysis of photoperiod sensitivity. We do not yet know whether the model has any contribution to make to the understanding of the biochemical mechanisms of photoperiod and temperature responses, but at the very least, it should provide the basis for indicating the most appropriate environmental conditions, genotypes and physiological stage of the plants most suitable for such investigations.
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Comparative analysis of sample preparation methods to determine the concentration of arsenic in soil- and plant-samples
167-170Views:477Arsenic contamination of the fields and groundwater is a global problem. Alföld is the most affected area in Hungary. Irrigation witharsenic contaminated water, and crop production on the contaminated soil can cause a food safety problem, because arsenic is easy taken up by the cell of the plant roots. To prevent this, very important to monitoring the arsenic content of soils and plants. Inductively coupled plasma mass spectrometry (ICP-MS) is a fast, easy method to determined the concentration of minerals in the case of plant and soil samples The analytical methods can give reliable, results if the analytical process, including the sample preparation method, is the best. The objective of this study was to compare 3 type of sample preparation method which was dry ashing, wet digestion in open system, and microwave digestion. As a result of our experiement shows the microwave digestion is the appropriate method to determined the arsenic content of soil samples. In the case of plant samples we can use wet digestion in open system or microwave digestion as a samle preparation method.
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Economic Assessment of Biodiesel Production for Hungarian Farmers
72-76Views:223Utilisation of oil of plant origin as a fuel is gaining acceptance in the European Union and elsewhere. Besides environmental protection, energy saving, and decreasing over-production of food. Additionally, the subsidisation of farmers and the development of rural sub-regions also contribute to its spread. This study specifically focuses on the direct effects biodiesel's raw materials and final products are now having on farmers, while reviewing and quantifying these effects. I have purposely restricted my analysis to these two elements of the biodiesel chain.
The biodiesel chain seems to be a great method for improving the economic and social position of participant farmers in many ways. Presently, the profitability of raw materials’ production looks to be the crucal point in the chain, and could be strengthened best with intensive, habitat-specific agrotechnic. It would only be possible to reach a favourable profit margin for farmers if yields reach unrealistic averages or if there is a significant hike of the 2000 producer’s price in the oil plant branch.
The main attraction of sunflower- and oilseed rape production lies in the stabilization of market conditions, which is not only gong to appear in oil plant branch but – thanks to the reduction of outputs – also in the cereal branches. Better economic safety for farmers may play a role at least on the same level as in plant production, which involves more risks than profit maximalization.
The reduction of the prime cost of biodiesel could be possible through the direct combustion of the whole oilseed plant or its residues or electricity production using them. Whereas energy demand for biodiesel production is low (appr. 5%) but it needs subsidization and the prices of natural gas and electrical energy presently look favourable in Hungary. Additionally harvesting and baling of the residues is technically problematic, which is why their use may seem to be reasonable just over the middle or long term. Another possible factor of cost reduction could be the centralization of some partial operations, which needs serious financial resources to reduce amortization cost per product, provided there be several biodiesel projects near each other during establishment. Creation and operation of a logistical system could also be a good method for improving the viability of the biodiesel chain, in order to optimize transport schedule and distances. However there are also some organizational difficulties in this case. -
Effect of N, P and K fertilisers and their interactions in a long-term experiment on winter barley (Hordeum vulgare L.)
87-92Views:315The aim of this work was to analyse the effect of K, P and N supplies on the yield of winter barley in a long-term mineral fertilisation experiment with clearly distinct soil nutrient supply levels in order to develop fertilisation guidelines for winter barley growers. The experiment was set up in 1989 on a chernozem meadow soil calcareous in the deeper layers, applying all possible combinations of 4 levels each of N, P and K fertiliser, giving a total of 64 treatments.
The results of analyses performed in 2011 and 2012 can be summarised as follows:
- In 2011, when rainfall supplies were deficient in the shooting phase, improved K supplies (324 mg kg-1 AL-K2O) increased the grain yield, but in 2012, when rainfall supplies were more evenly distributed, K supply levels in the range 210–335 mg kg-1 AL-K2O had no significant influence on the yield of winter barley.
- An analysis of the P treatments revealed that, compared to the 119–133 mg kg-1 AL-P2O5 level (P0), better P supplies (186–251 mg kg-1) led to a significant increase in the grain yield.
- In both years rising N rates significantly increased the yield up to an annual N rate of 160 kg ha-1.
4. A K×N interaction could only be detected in the nutrient supplies of winter barley in 2011. The yield-increasing effect of N fertiliser was more pronounced at better K supply levels, while K fertiliser led to higher yields in the case of better N supplies.
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The effect of drought and cropping system on the yield and yield components of maize (Zea mays L.)
51-53Views:297Different Cropping Systems have many advantages and ensure better crop growth and yielding. Its combination with other agronomic measures can ensure optimal crop density for maximum crop growth and photosynthesis efficiency. The aim of this study was to investigate the influence of different cropping systems on monoculture and biculture rotations [maize- wheat]. The study found that crop rotation does not have a significant effect on the grain nutrition quality, Leaf Area Index (LAI) and Normalized Difference Vegetative Index (NDVI) but has a significant effect on the Soil-Plant Analysis Development (SPAD). Yield and yield components were significantly influenced by crop rotation in this study as yield, plant height, cob weight and number of grains per row all recorded lower mean at 5% probability levels.
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Evaluation of bioactive substances in different parts of the root in beetroot (Beta vulgaris ssp. esculenta var. rubra)
33-38Views:351Beetroot, also known as Beta vulgaris ssp. esculenta var. rubra, is a root vegetable that contains a substantial quantity of bioactive elements, notably antioxidants and anti-inflammatory properties. These bioactive compounds, including betalains, phenolics, and flavonoids, contribute to the health-promoting properties of beetroot. The antioxidant and anti-inflammatory effects of beetroot have been extensively studied in preclinical and clinical settings. The active compounds in beetroot have been reported to provide benefits in reducing the risk of various diseases, our research provides a comprehensive analysis of the bioactive compounds the inner and outer skin parts of the root structure. The findings aim to contribute to a deeper understanding of the potential health benefits associated with specific beetroot root components. Furthermore, the results have implications for optimising beetroot cultivation and processing for enhanced nutritional value. This research not only advances our knowledge of the phytochemical profile of beetroot outer skin but also offers valuable insights into the broader field of plant biochemistry and its applications in promoting human health and nutrition.
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Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:558Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested. -
The analysis of ostrich chick vitality
35-40Views:462Examinations on ostrich chick vitality can help to improve the effectiveness of Hungarian ostrich husbandry and hatching technology. The investigations were carried out on an ostrich breeder farm in Eastern Hungary. For the analyses, the Tona et al. (2003) scoring system was applied presenting eight main criteria (activity, feather, condition, eyesight, the condition of navel and leg, the amount of the remaining shell membrane and egg content, and the size and tenderness of the abdomen). Most chicks (98.08%) were vigilant and had clean and dry feathers. There was no difference between the average hatching weights between May and August but a decreasing tendency was shown during the laying season. The vitality total score was above 90 in each evaluated month and was not affected by the hatching month. Our results revealed that the vitality of chicks was not affected by either the hatching month (P=0.51) or the weight category (P=0.11). Neither the hatching weight of chicks and leg condition were correlated (P=0.79). Results showed that the Tona scoring system with small modifications can be well applied to ostrich chicks. Practical on-farm usage of the system can be suggested as an aid in day-old chick evaluation. After individual marking of chicks, the investigation of growth and culling rate could be carried out for more precise conclusions, involving more farms and laying seasons.