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Study of factors controlling the amount of 0.01 M CaCl2 extractable Norg fraction
437-449Views:335The use of new methods describing the “readily available” nutrient content of the soil is spreading on a global scale. The 0.01 M CaCl2 extractant is a dilute salt solution in which the easily soluble inorganic (nitrate-N and ammonium-N) and organic N fractions, P, K and micronutrients are also measurable. The 0.01 M CaCl2 has been tested in the University of Debrecen, Institute of Agricultural Chemistry and Soil Sciences since the 90’s. The results of the researches related to organic N fraction, performed in the last decades, and the results of the present study (originating from the long-term experiment of Karcag, 2007–2009) can be concluded as follows:The measurement of easily soluble and oxidizable organic nitrogen (Norg), besides inorganic fractions, could improve the nutrient management.The amount of the Norg fraction is determined by the soil conditions, therefore it is considered to be a site-specific parameter.Management practices and cropyear affect the amount of Norg as well. The present research confirmed that, the effect of fertilization on the amount of Norg can be explained by the changing of the yield (related to total biomass production), while the effect of cropyear is related to the differences in mineralization circumstances and yield as well.The measurement of the Norg fraction is increases the accuracy of N-supply, therefore it could prevent the environmentally harmful excess N application as well. -
Effects of combined nutrient supply treatments on some physiological parameters of autumn wheat
241-251Views:310The Fleischmann Rudolf Research Institute in Kompolt is not only famous for plant breeding but the institute also surveys the effects of different nutrient supply methods since 1918. In 2017, we joined this research supported by EFOP 3.6.1 project. Our aim was to investigate photochemical processes – which is one of the most determinant in case of yield – of crops by in vivo field measurements. We measured the chlorophyll content of leaves using Minolta SPAD 502. We used miniPAM fluorometer to determine actual photochemical efficiency and non-photochemical quenching of PSII during natural light conditions and also to evaluate the pigment (chlorophylls and carotenoids) and water content of leaves we applied field spectrophotometer (ASD FieldSpecPro 3). We utilized these methods by various treatments (1. treatment with soil bacteria + head and base fertilizer; 2. treated by only head fertilizer; 3. treated by only base-fertilizer) in field experiment of autumn wheat (4.1–2.43–1.19 ha) in June, 2017. The difference between treatments was clearly detectable. In the case of the first treatment, physiological processes were more intense and the ripening occurred earlier. The obtained yield was the highest in the case of the area treated by soil bacterial. Based on the results, the first treatment can be recommended in practice.
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Fast neutron irradiation long term effect on Ocimum basilicum germination and initial vegetative parameters
23-31Views:234The goal of this study is to assess the long-term (3-year) effects of rapid neutron irradiation using an Americium-Beryllium source on two generations (M0 and M1) of Ocimum basilicum commercial trait. Seeds of each variety and generation were irradiated with 0, 7.5, 20, 40, and 50 Gray in 2018, and then seeded in 2021 – with their corresponding controls – to see how the different irradiation treatments affected germination and early vegetative metrics three years after irradiation. In the first generation, no significant changes between the treatments were seen. Increased irradiation dosage resulted in a significant decrease in germination % in the second generation (M1), but a significant increase in seedling height. The M0 generation seedlings, on the other hand, were substantially shorter than controls. In the M0 generation, the higher dosages (40 and 50 Gray) were deadly, and seedlings were terminated 7–10 days after germination. The irradiation dose of 20 Gray was shown to be the most successful in inducing viable and beneficial alterations for phenotypic characteristics in basil. When compared to control plants, the lower (7.5 and 20 Gray) bombardment generated leaf anomalies such as smaller leaves and internodes, a split apex, and a ribbon-like stem.
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Climatic water balance in Hamelmalo, Eritrea
69-76Views:471Agricultural production is an important sector for peoples to live, but it is highly affected by climate change. To have a good production we need to understand the climatic parameters which adversely affect production. Hamelmalo, which is located in the semi-arid area of Eritrea, is vulnerable to climate change and this is realised in the total production loss. Nevertheless, there is no concrete reference about the climate of the region due to lack of data for a long time. Changes in precipitation (P), evapotranspiration (ET) and, implicitly, in the climatic water balance (CWB), are imminent effects of climate change. However, changes in the CWB, as a response to changes in P and ET, have not yet been analysed thoroughly enough in many parts of the world, including Eritrea. This study also explores the changes of the CWB in the Hamelmalo region, based on a wide range of climatic data (P, relative air humidity and evaporation pan necessary for computing potential evapotranspiration (PET) with the pan evaporation method) recorded at Hamelmalo from 2015-2019. This analysis shows that the annual cumulative CWB for Hamelmalo is negative in 67% of the years. The dry season without precipitation leads to negative CWB and the change in CWB only starts from the raining or crop season. Based on this recent study, 2015 had the highest PET and lowest P, and this resulted in the lowest CWB in the investigated period. Opposite to this, 2019 had lower PET and highest P, which led to the highest CWB. However, the monthly values of CWB did not correlate with the annual P or ET. On the base of our study, it can be concluded that PET and P were very variable in the investigated years and P was the most influential elements of CWB.
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Foreword
9-10Views:494The higher education of economics and agriculture in Debrecen started 150 years ago. The historical roots of our institution reach back to the middle of the 19th century. In the 1850s, the wise leaders of the city of Debrecen realised that the development of agriculture is the sole opportunity which can make the region of the Tisza River and the Trans- Tisza region the most valuable pantry of the nation. Due to the generous support of the citizens of Debrecen, as well as the enthusiastic work of scientist and teachers, the National Higher Education Institute of Farming was established on 22nd October 1868 as the first agricultural higher education institution of the Great Hungarian Plain.
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Effects of cultivation practices and hybrid selection on endofusariosis and mycotoxin contamination in maize
69-78Views:465The aim of this study was to determine the levels of deoxynivalenol (DON) and fumonisin mycotoxins in tillage and no-till systems in 2020 and 2021. Additionally, we sought to establish the levels of internal Fusarium infection in different tillage systems. We examined four tillage systems for two years (conventional tillage (plowing), conservation tillage, reduced tillage, and strip-till). The results indicate that toxin levels varied between the two years, with fumonisin production dominant in 2021, while DON toxin production was dominant in 2020. Regarding internal Fusarium infection, the lowest levels were observed in the plowing system in 2020, whereas in 2021, the lowest levels were measured in the reduced tillage system. Like the low DON levels in 2020, the plowing-based tillage system resulted in the lowest fumonisin levels in 2021. Throughout our experiment, the toxin levels were below the permissible limits for unprocessed corn, with DON levels under 1750 μg kg-1 and combined fumonisin B1 and B2 levels under 4000 μg kg-1. However, in areas where toxin contamination is typically problematic, considering the beneficial impact of plowing on reducing toxin contamination might be advisable when planning tillage.
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Drought cycle tracking in Hungary using Standardized Precipitation Index (SPI)
97-101Views:593Drought is one of the natural hazard risks which badly affects both agricultural and socio-economic sectors. Hungary, which is located in Eastern Europe has been suffering from different drought cycles; therefore, the aim of this study is to analyse the rainfall data obtained from ten metrological stations (Békéscsaba, Budapest, Debrecen, Győr, Kékestető, Miskolc, Pápa, Pécs, Szeged, Siófok, Szolnok) between 1985 and 2016, by using the Standardized Precipitation Index (SPI).
The results showed that 2011 was recorded as the worst drought cycle of the studied period, where the SPI ranged between -0.22 (extreme drought) in Siófok, and 0.15 (no drought) in Miskolc. In a similar vein, the study highlighted the year 2010 to be the best hydrological year, when the SPI reached 0.73 (mildly wet) on average. Interestingly, the Mann-Kendall trend test for the drought cycle showed no positive trends in the study area. Finally, more investigation should be conducted into the climate change spatial drought cycle in Europe.
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Does drought stress always negatively affect the yield and quality of soybean in Hungary?
37-40Views:453Globally-fluctuating climate imposed serious abiotic stresses on the agricultural sector, leading to noticeable, and sometimes disastrous, losses in yields and/or quality of crops; however, in certain cases, plants could survive stress with relatively low reductions, and sometimes even with some enhancements as a reaction to changed environment, especially in the case of mild stress. An experiment was conducted in 2017 and 2018 in Debrecen, Hungary to evaluate the mild drought stress influence on the yield and quality of three soybean cultivars. The results showed that both ES Pallador and Pedro cultivars could achieve more yield when subjected to mild drought conditions; however, protein concentration was enhanced in ES Pallador whereas slightly degraded in Pedro under drought. The cultivar Pannonia Kincse followed different trend; both yield and protein concentration were reduced under drought. Oil concentration of the three cultivars did not show significant changes; however, it always followed opposite trend to that of protein concentration. It could be concluded that both ES Pallador and Pedro are recommended to be grown under rain fed conditions in Debrecen, whereas Pannonia Kincse is recommended under irrigation conditions.
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The R&D and innovation activity of agribusiness enterprises in Hajdú-Bihar County
67-71Views:220Economic changes have significantly accelarated in the 21th century. In this turbulent market environment enterprises are forced to adapt continously as they must be flexible in order to meet changing market needs. To achieve flexibility companies require innovation. The economic relevance of innovations is significant in every market sector and agriculture is no exception. Re-dynamizing agricultural innovations is a possibility for the outburst of the Hungarian agriculture. Agricultural enterprises have to face competitors as well and they should give priority to efficiency, sustainability and competitiveness in order to preserve their position in BOTH global and domestic markets. This study examines the innovation potential of the agricultural enterprises in the North Great Plain region based on own databases and case studies.
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Rheological evaluation of industrial tomato
137-140Views:303Today, tomato has the second largest harvesting area globally, and the fourth largest in Hungary. The importance of industrial tomato is growing year by year, and one of the most important tasks is to satisfy the needs of the industry. To meet these needs, the producer has to find the proper genotype, which can achieve up to 100 t ha-1 crop yield. This quality has high importance to be researched.
The experiment was carried out in the Botanical and Exhibition Garden of the University of Debrecen, with 3 examined genotypes. The planting date was 3 of May 2018. The harvesting was two-threaded. On 27 of July the physical parameters – fruit shape index (length/diameter), firmness (kg cm-2), water-soluble dry matter content (Brix%) and weight of the berries (g) – were evaluated. On 15 of August the rheological parameters were examined – the force needed to tear the skin – bioyield point (g) and flesh firmness (g).
The fruit shape index was oval (between 1.19 and 1.24) for all the examined genotypes. The firmness was between 0.92 (UG1122713 F1) and 1.05(Prestomech F1) kg cm-2, which compliance the needs of the industry. The water-soluble dry matter content was suitable, with a value between 4.49 (Prestomech F1) and 4.65 (UG1122713 F1) Brix%. The weight of the berries was between 45.94 (UG989 F1) and 49.37 (Prestomech F1) g per piece.
The bioyield point was between 76.00 (UG1122713 F1) and 85.16 (UG989 F1) g. The flesh firmness was between 33.68 (UG1122713 F1) and 42.81 (UG989 F1) g.
Finally, we can conclude based on the obtained data that the UG989 F1 hybrid was the most proper for the applied cultivation conditions. This genotype can be recommended for the farmers even in extreme weather conditions.
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Leaf reflectance characteristics and yield of spring oat varieties as influenced by varietal divergences and nutritional supply
29-34Views:274Inadequacy of nutrients in the soil and sufficient inaccessibility to nutrients is caused by factors that affect production and productivity of spring oat varieties. Exogenous application of nutrient and real time nutrient assessment can therefore reverse these associated negative consequences. A field experiment was conducted to evaluate the response of spring oat varieties to sulphur and silicon based fertilisation. Eight spring oat varieties, four level of nutrient application was arranged in a split-plot design with three replications. The obtained results showed that foliar application of sulphur improves the grain yield of most nutrients responsive varieties by about 34.7%. However, application of silicon had shown a diminishing return association to grain yield of variety GK Kormorán, GK Pillangó, Lota, Panni. LAI, thousands grain weight, SPAD, NDVI was significantly (p < 0.05) influenced by genetic difference of the tested varieties, developmental plasticity, and nutrient application. Significantly higher grain yield was obtained from the variety Mv Pehely than the other tested varieties. Therefore, it could be inferred that a combined use of nutrient responsive spring oat varieties and sulphur containing fertilisers could be important agronomic practice to improve grain yield and to develop climate resilient oat varieties.
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Physiological traits and yield of three soybean (Glycine max (L.) Merr.) genotypes as affected by water deficiency
11-15Views:491Soybean is a very important legume; it has the highest protein content, and it is a very important source of vegetable oil. Soybean is droughtsusceptible, and drought is one of the major abiotic stresses that has been increasing over the past decades as a result of the global climatic changes. To evaluate the influence of drought stress, three soybean genotypes were grown under rainfed conditions, and compared to irrigated controls. The obtained results showed that the chlorophyll content, leaf area index and plant height decreased under drought stress conditions, which led to noticeable and sometimes significant yield reduction. Our results suggest more specific studies on the physiological changes of the local soybean genotypes under drought stress to better select the adopted ones.
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Effect of Moringa leaf extract and set size on the bulb weight, diameter and yield of onions (Allium cepa L.)
127-131Views:725Studying the use of natural extracts or biostimulants in improving vegetable plants is the current needs as an alternative way to the use of chemical products. This experiment was carried out to evaluate the effect of Moringa oleifera leaf extract (MLE) as a natural growth regulator on bulb diameter, weight and yield of onions ‘Texas Early White’ cultivar. Therefore, three concentrations (control, 2%, 4% and 6%) of MLE and three different onion set sizes [small (3±1 g/set), medium (6±1 g/set), and large (10±1 g/set)] were prepared in a randomized complete block design with three replications and plant spacing 20×15 cm. MLE was applied as a foliar spray directly onto the plants with the amount of 25 ml/plant every two weeks from sprouting to maturity. The achieved results indicate that the interaction of foliar spray with moringa leaf extract 4% and large set size of 10±1 g/set gave the highest value of the total yield 4802.7 g m-2, while the lowest yield 1531 g m-2 was recorded in the interaction of control and the smallest set size of 3±1 g/set. Also, a significant difference was recorded in bulb diameter separately. Thus, the largest bulb diameter 68.90 mm was obtained from the planting of the largest set size 10±1 g/set with 4% of MLE while the smallest bulb diameter 38.40 mm was recorded from the smallest set size 3 ±1 g/set with control treatment. Similarly, a significant effect was recorded in both set size and MLE separately. The highest bulb diameter 58.71 mm was achieved in the planting of the largest sets 10±1 g/set, while the lowest diameter was 42.12 mm in the planting of the smallest set size 3 ±1 g/set. Similar results were recorded for the bulb weight and total yield. The concentration of 4% MLE produced the highest bulb weight 99.74 g/bulb and yield 3324.5 g m-2, while the lowest bulb weight and yield was obtained in the control treatment 55.61 g/bulb, and 1869.3 g m-2. Consequently, the results indicate the positive effect of Moringa leaf extract which can be applied as a natural substance in the form of foliar spray at critical growth stages to improve the growth and yield of dry onions.
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Comparison of sample preparation methods for the identification of Staphylococcus Aureus by MALDI-FOF MS
9-14Views:539Coagulase-positive staphylococci include 3 species, Staphylococcus aureus, S. hyicus and S. intermedius. Of these three species, S. aureus is the most well-known human pathogen. S. aureus is part of the human and animal normal microbiota, however, it is capable of producing several staphylococcal enterotoxins (SEs) that cause intoxication symptoms of varying intensity in humans after consuming contaminated food. Selective media which are used for the determination of coagulase-positive staphylococci from foods are not able to identify isolates at a species. With the MALDI-TOF MS technique, we can identify S. aureus cheaper and faster than by using molecular methods. This paper describes the results of the study of the presence of coagulase-positive staphylococci and S. aureus in many food products, and the application of three sample preparation methods: direct sample preparation, formic acid suspension and ethanol extraction.
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Growth and yield patterns of black locust (Robinia pseudoacacia L.) sample trees affected by site conditions: case studies
125-128Views:338The trees removed from the long-term experiment plots are available for the measurements as lying trees. Through the determination of the volume in sections along the stem, the stem form, the stem volume and other factors can be specified. The comparison of the stems of individual trees of first and third yield classes of black locust (Robinia pseudoacacia L.) stands shows that site conditions have a main effect on the yield (mean tree volume). The difference can be as high as 53% at the age of 30 depending on the sites. To determine the growth patterns based on tree volume is rather a new approach in the light of the relevant literature. The obtained results also highlight the importance of choosing the appropriate tree species for a given site.
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Validation of the competence profile of agri-environmental engineering bachelor course
63-71Views:326As member of the European Union, the development if the Hungarian agriculture is determined by the Common Agricultural Policy. After the reform of the Common Agricultural Policy in 2003, the environmental protection has got a crucial function. The constant changing role of agriculture has reacted students in higher education. For firms connected to agriculture, human resource management based on competency has an important role in custody of competitiveness. One of the most important goals of firms is to supply the demands of their economic partners. To make it successfully, highly qualified human resource is required. The quality of human resource is determined not only by its qualification, language skills, professional experience, and practise, but extant competencies that can also be developed. To choose the expectant employee, it is not enough for them to have intelligence and proficiency; it is also decisive to examine their particular competencies. All of these will define the employee’s
achievement that will definitely contribute to the success of the enterprise. Basically, closer cooperation is needed between higher education institutions and employers to achieve this goal. At the same time, the higher education institutions can revise their training systems considering the actual requirements of labour market, and accordingly, they can form syllabus in a way to train auspicious experts. In this article examination of the requirements of labour market for graduates from agri-environmental engineering bachelor course is presented. -
Some strategic aspects of animal protein production
11-19Views:303The access to food shaped human societies and dietary models throughout the history of mankind. Animal protein consumption became a part of human culture. Data are presented showing the relationship of daily calorie and animal protein consumption as affected by capita GDP changes. Examples are presented how genetic improvement of animal and fodder plants influenced the resource efficiency and the overall environmental footprint per unit product. The two examples presented are: the dairy industry of the USA the 1944 and 2007 situation, and the Hungarian broiler chicken sector considering data relevant to 1930, 1960 and 2010. In both cases, dramatic improvements in resource efficiency could be demonstrated. The agricultural area required to animal feed production was reduced by more than 80% in both cases per unit product. Future possibilities are briefly discussed, referring to the still unutilized land reserves of the Globe, the new evolving technologies in progress inclusive the CASPR/Cas 9 genetic editing methods.
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Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
153-157Views:473Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.
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Evaluation of Lipizzaner mares’ fertility data in 10 Hungarian stud farms
23-26Views:139Despite numerous studies on the genetic background and performance traits of the Lipizzan breed, fertility characteristics of Hungarian Lipizzan mares have not been systematically examined. This study evaluates the reproductive performance of 220 Lipizzaner mares from 10 Hungarian stud farms, born during the period of 1981 to 2019, using breeding records from 1998 to 2022. The mares were grouped into 25 mare families and 24 birth cohorts. Data on successful pregnancies and foalings were analyzed with respect to age, family affiliation, and breeding year. Results show that mares aged 8–12 years had the highest fertility rates, while a gradual decline was observed after 15 years. Breeding activity increased markedly after 2011, peaking in 2022 with the highest number of matings and foals born. Fertility varied considerably among mare families: some achieved consistently high reproductive success, while others showed low or zero foaling rates despite repeated matings. Hungarian mare families generally produced better fertility results, whereas Fogaras families displayed greater heterogeneity. The study emphasizes the importance of monitoring reproductive efficiency in small mare families, as unfavorable fertility trends may jeopardize their long-term viability. These findings contribute to improving breeding strategies and ensuring the preservation of genetic diversity in the Hungarian Lipizzaner population.
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Lack of polymorphism of the agouti signaling protein (ASIP) gene among four different brown hare (Lepus europaeus Pallas 1778) populations
81-85Views:387The brown hare (Lepus europaeus Pallas 1778) is a common palearctic and a popular game species therefore it has been an obvious subject for population genetic studies since the second part of the 20th century. Among the several mitochondrial DNA studies some have been carried out concerning nuclear genes as well. The agouti signaling protein gene (ASIP) is involved in regulating the synthesis of eumelanin and pheomelanin in melanocytes of mammals. Though many studies focused on it in relation with several mammalian species, minimal information is available on this topic concerning the brown hare.
Here we present a short communication concerning the agouti signaling protein (ASIP) gene in four different country’s L. europaeus populations, namely Lithuania, Hungary, Serbia and Georgia. N=45 tissue samples have been investigated from overall 17 sampling sites of the different countries. There has not been found any polymorphism among the sequences. In an alignment with other Leporid species’ partial ASIP sequences downloaded from ENA we have found that based on a 178 base pairs long DNA sequence the haplotype of our samples contains three other Lepus species as well. This is concordant with the findings of a previous study focusing predominantly on the European rabbit (Orycto lagus cuniculus Linnaeus 1758) and the several mutations of its ASIP gene.
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Mitochondrial DNA-based diversity study of Hungarian brown hares (Lepus europaeus Pallas 1778)
23-29Views:358The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.
The aim of our large scale long term ongoing study is to fill this gap of information on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.
This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.
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Folic acid content of beetroot leaf and root by different growing stages and genotypes
115-119Views:537An increasing interest has been observed of beetroot leaf as a salad component due to recent studies focusing on their nutritional value. The randomized field experiment was carried out on lowland chernozem soil with 6 varieties, 3 replications and 2 sowing dates. Sampling was performed on 23 of August 2018 at the stage of 30 and 50 days of vegetation, where leaf (30 and 50 days) and root (50 days) were collected. Total dry matter, folic acid and nitrate content were evaluated.
The results of this investigation show that higher total dry matter content was measured in the root (8.47–10.30%) compared to the leaf in both developmental stages (6.47–9.20%). Nevertheless, higher folic acid content was found in the young leaves of 30 and 50 days of development (58.77–113.86 µg 100g-1). Among the examined varieties, Bonel has presented great amount of folic acid not only in the leaves (99.35–113.61 µg 100g-1), but also in the root (89.99 µg 100g-1). Finally, lower nitrate content was found in Libero (316.16 mg kg-1) at 30 days and in Akela (340.41 mg kg-1) at 50 days of development. Thereby, fresh consumption of beetroot leaves are highly recommended.
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An overview of swine production and marketing in Africa - Mini review
121-129Views:609Pigs are the oldest domesticated animals, though their ancestry is still uncertain because of a lack of archaeological and genetic evidence. Pigs serve a vital role in reducing the demand for livestock and livestock products in most parts of the world. Especially in the African continent, pigs serve as mobile cash implying how easily they can be converted into cash to sort pressing family needs. Pig production in Africa is different from advanced systems of production in temperate countries, however, it has similarities with most of the tropical low and middle-income nations. Pig production in Africa requires attention to enable it to contribute effectively to the growing African population. However, it has been orchestrated with a myriad of challenges including market access. This mini-review is designed in a manner to provide an overview of pig production contribution to livelihoods in Africa and associated challenges with possible appropriate solutions.
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The effect of season on the microbiological status of raw milk
95-99Views:499Many factors can influence the microbiological quality of raw cow’s milk. In this study, our aim was to determine whether there was any difference between the microbiological statuses of milk produced in different seasons. Samples were collected and analysed from five dairy farms in Hajdú-Bihar County, from February to November in 2019. During our studies, total plate count (TPC), coliform count and Staphylococcus aureus count of raw cow’s milk samples were determined.
There was no significant difference (P>0.05) between the mean TPC values detected in the milk collected in winter and autumn, but that values were significantly (P<0.05) lower than in the milk samples collected in spring and summer. Similarly to the TPC, in the case of coliform bacteria the lowest mean colony count was detected in the samples collected in winter. The difference was significant (P<0.05), compared to the values observed in the samples collected in summer. S. aureus was detected in bulk milk of only two farms in excess of 1.0 log10 cfu/ml. Also in case of S. aureus, there was a significant difference (P<0.05) between the values observed in the samples collected in winter and in summer. Samples from spring and summer contained the highest amount of S. aureus.
Based on the results of our studies, in the case of almost all farms the mean TPC, coliform and S. aureus counts were lower in the samples collected in winter, than in the samples collected in summer. The fact that the samples collected in winter contained the lowest amount of colonies could be attributed to the inhibition of growth of mesophilic microorganisms below 8 °C. Furthermore, the fact that we observed the highest colony counts in samples collected in summer, can be related to the heat stress of cows during the summer due to unfavorable weather conditions (high temperature and humidity).
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Analysis of different fertilization settings’ effect in the case of the summer savory’s (Satureja hortensis L.) yield and active agents
101-105Views:312During our research we investigated the Mediterranean summer savory’s (Satureja hortensis L.) nutrient requirement with different fertilization settings in a small plot trial. We measured the medicinal plant’s raw and dry drug mass, and the presence of the main essential oil active agents under the different fertilization settings using SPME (Solid phase microextraction) and GC-MS (Gas Chromatograph mass spectrometer).From the drug’s raw and dry mass perspective, based on the obtained results, in our opinion, the N30P40K60 nutrient requirements were ideal in 2015, while the N60P80K120 nutrient requirement showed the best values in 2016. A significant relationship is assumed between the different fertilization settings and the essential oil active agents’ distribution. During the analysis of data, we compared three of the savory’s essential oil active agents’ percentage of presence in the nutrient requirement samples.