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RAPD analysis of grapevine hybrids and cultivars
63-66.Views:180Utilization of the Randomly Amplified Polymorphic DNA (RAPD) technique as a molecular marker was tested to investigate the relationships between some representative grapevine cultivars and hybrids established at the Department of Genetics and Plant Breeding (CUB), to distinguish clones as well as to characterize various hybrids between species or cultivars and their parents. Vitis vinifera cultivars were easily and successfully distinguished by the RAPD technique and they were grouped according to the traditional taxonomic classification. RAPD patterns of the examined Pinot gris clones proved to be completely identical. Number of generations was reflected by the value of genetic distance of the examined hybrids. Genetic identity of parents and their offsprings was influenced by the selection applied in the process of plant breeding. Parental phenotypic and morphologic characteristics showed high degree of segregation in hybrids, but RAPD analysis revealed that their genetic similarity is considerable. The three Vitis anntrensis clones were properly discriminated from every cultivar and hybrid of Vitis vinifera, i.e. hybrids are much closer to the cultivated grapevine than to V. anzurensis due to the phenotypic selection carried out during the life-cycle of one or two generations.
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Inheritance of fruit colour and of shoot's pigmentation in the case of interspecific raspberry hybrids
33-36.Views:114In our research, fruit colour and the shoot's red pigmentation were evaluated in the hybrids of Rubus idaeus and Rubus parvifolius crossings. Y and Ys genes beside the T genes determine the fruit colour of interspecific hybrids, which is characteristic for raspberry. For the explanation of the significantly higher results of segregation then expected at the yellow fruit colour hybrids, we have supposed the presence of a second yellow gene (Y2). In the yellow colour, a lot of different shade colours can be identified from light yellow to the apricot colour. In the regulation of the production of yellow and red colour, several other genes can participate also. Identification of these genes would require more additional research. The C gene determines the shoot colour of raspberry and in the case of wild raspberries we have revealed the role of a dominant Pr gene. The Y and Pr genes are descended linked. The value of crossing over is approximately 15%. The anthocyanin production inhibitory effect of the Y gene extends only for fruit. At the shoots of yellow fruit plants, strong anthocyanin production was observed.
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Reactions of some cucumber (Cucumis sativus L.) lines and hybrids to zucchini yellow mosaic virus (ZYMV) and selection of tolerant breeding lines
66-68.Views:172In the past years zucchini yellow mosaic virus (ZYMV) has been appeared as a new pathogen of cucurbitaceous plants in Hungary. It caused severe disease outbreaks on the fields of pickling cucumber hybrids which are highly tolerant to cucumber mosaic virus (CMV). Preliminary inoculation tests have showed that all of the important pickling cucumber hybrids produced in Hungary were susceptible to ZYMV-P. However, a selection of Chinese Long and the breeding line I- KS 10C proved to be highly tolerant. The Fl plants of the crosses between the tolerant lines and the susceptible genotype H I were susceptible to ZYMV-P. F2 populations segregated for susceptible and tolerant individuals at a ratio of 3:1. The results strongly suggest that the resistance to ZYMV in our sources is controlled by a single recessive gene. Inoculations of the ZYMV and CMV susceptible cultivar Budai csemege and the CMV tolerant hybrid Perez Fl with the complex of ZYMV+CMV resulted extremely severe symptoms (strong mosaic and necrotic spotting of the leaves) on both cultivars. On the Chinese Long line, which is tolerant to ZYMV and CMV, respectively, the complex of the two viruses caused mild symptoms. The results show a synergistic pathological effect of ZYMV and CMV on differrent cucumber genotypes. More detailed studies on the interactions among the plant genotypes, viruses and virus strains are needed to develop cucumber hybrids that are highly resistant to the ZYMV+CMV complex.
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Antioxidant characterization of apricot (Prunus armeniaca L.) cultivars and hybrids
47-51.Views:227This report forms a part in our long-term study dedicated to reveal the antioxidant properties of apricot fruits through several years. Nineteen apricot cultivars and 8 hybrids were comparatively analysed. Total phenol content and antioxidant activity showed a good correlation. The tested cultivars and hybrids could be arranged in three groups representing different antioxidant activities, 12 entries were involved in the group with relatively low antioxidant capacity (FRAP value < 1500 mmol/L); 10 accessions were classified in the group with medium antioxidant capacity (1500 mmol/L < FRAP value < 3000 mmol/L); and 5 genotypes were grouped to the category of high antioxidant value (FRAP > 3000 mmol/L). `Morden 604' produced a surpassingly outstanding antioxidant character. H-donating ability has further supported our results. Phenolic substances were accumulated and ferric reducing ability was increased in the ripe fruits compared to the unripe ones. High levels of variations in the total phenol content and antioxidant capacity in of apricot fruits were revealed in this study. Environment, year or rootstocks may also influence the antioxidant properties of fruits. however it seems to be convincing that crossing parents with outstanding antioxidant character can produce hybrids with higher antioxidant capacity. Hungarian apricots are widely appreciated for their premium flavour and — as it was evidenced in this study — for their relatively good antioxidant properties. However, delicious fruits should be made functional foods having beneficial health effects through accumulating large amounts of antioxidant molecules in the fruit flesh.
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Rootstocks for Cherries from the Department of Fruit Science Budapest
63-66.Views:196Cherry rootstock breeding started at the Department of Fruit Science, SSU Budapest by the late 50-s and the activity can be divided into three main groups. In the first stage the activity was focused on collection of native mahaleb cherry (Prunus mahaleb L.) varieties lead by L. Sebők. After evaluation in the nursery and orchard tests there are four promising rootstock cultivars selected from this material: 'Korponay' used as self fertile seed tree, its seedlings are recommended for sour cherries. The mahaleb varieties 'Bogdány' (vigorous), 'Egervár' and 'Magyar' (medium vigour) are propagated by cuttings. The next project has started in 1979 with the aim to select self fertile mahaleb seed trees producing homogeneous seedling populations with reduced vigour. Inbred populations from isolated flowering self fertile trees were produced and planted out in 1980. The inbreeding of 'Korponay' self fertile P. mahaleb variety resulted in specimens with different fruit colour (yellow, red, black), fruit shape and size. From among them self fertile trees were selected with various growth characteristics. Seedlings of that self fertile mother trees (S2 population) were tested in seedbed, they showed homogeneous phenotype characteristics as liners in the nursery. As rootstock of 'Érdi bőtermő' sour cherry in the orchard most of the S2 lines proved to be less vigorous in comparison to SI popuplations. 'Érdi bőtermő' trees budded on certain S2 lines in the orchard are more productive than those on S1 ('Korponay' seedling). Characteristics of the S2 generation as seed tree were studied as well. We expect to get morphologically homogeneous seedling populations with different growth vigour and good productivity in the later inbred generations. In the last couple of decades the research activity concerning ground-cherry and its hybrids resulted in dwarfing rootstocks. Prunus fruticosa Pall. hybrids from the natural flora of Hungary were collected and artificial hybrids were created between P. fruticosa and mahaleb cherry. Most of them are in the initial tests, only one of them is before registration, named 'Prob', which is a dwarf rootstock for sweet cherry. By the screening of new hybrids medium vigorous or semi dwarfing and precocious rootstocks seem to be promising for the cherry industry.
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Efficacy of ethrel (2-chloroethyl phosphonic acid) as a chemical hybridizing agent in red pepper (Capsicum annuum L. var. Pusa jwala)
41-44Views:181A field experiment was conducted during 2003-2004 and 2004-2005 to study the effect of foliar sprays of ethrel or ethephon (2- chloroethyl phosphonic acid) on pollen sterility and yield parameters in Capsicum annuum var. Pusa jwala. Effect of treatments was also studied in F1 hybrids raised from treated male sterile plants crossed with the control plants. Plants sprayed with 0.1, 0.2 and 0.3% (v/v) ethrel exhibited 93.1-100% pollen sterility. This was associated with significant reduction in yield parameters (number of flowers, fruits/plant, fruit size, number of seeds/fruit and total yield/plant). However, the plants sprayed only once with 0.1% ethrel at pre-meiotic stage showed 93.1% pollen sterility without any significant reduction in yield parameter. The F1 hybrids obtained by crossing the 100% male sterile treated plants with the pollen of untreated (control) plants exhibited only insignificant reduction in the number of flowers/ plant, fruits/plant, fruit size, number of seeds/fruit and total yield/plant. However, these parameters in F1 hybrids were significantly higher over the treated plants.
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Book review
37-39.Views:193Plum is a significant temperate fruit and a very important fruit species in Hungary as well. Cultivation has moved beyond the area boundaries of the Northern Hemisphere many centuries ago. Domestic (European) plum production has been particularly affected by the pandemic-scale destruction of the Sharka virus and worldwide breed changes. According to FAOSTAT (2016) data, world plum production is 12 million tones, with 36% from Eurasian, 63% from Japan and other Asian varieties. The share of American plums is only 1%. Domestication and dissemination of plums is „multi-stepped” because homemade (taste) plums are hybrids of two nature species in the first place, but Japanese plums (hybrids with Chinese plums or Prunus cerasifera) are not uniform; the role of the American plum species is much smaller, though their prospects cannot to predict with certainly. The book consists of 19 chapters, finding a complex way of summing up linguistic, historical, floristic, historical-botanical, cultivation, and morphological and anatomical knowledge.
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Sensory evaluation of white asparagus (Asparagus officinalis L.) hybrids
51-55.Views:338Comprehensive system of sensory evaluation was developed to obtain information on the different varieties by determining asparagus quality parameters. 9 sensory characteristics were estimated to describe flavour differences. The tests were carried out in 4 varieties of white asparagus (Asparagus officinalis L.) grown on brown humus sand soil by etiolate method in ridge cultivation. The experiment was performed at the University of Debrecen in Debrecen-Pallag. To increase the Hungarian asparagus consumption, a classification of varieties
regarding to asparagus flavour is necessary. In our experiment it could be stated that there are strict correlations (0.892) between the sweetness – total impression and between lacks of unpleasant aftertaste – total impression (0.870). The result of surveys can lead to higher popularity of the asparagus. These results are presented in order to recommend the varieties to producers for a successful marketing. The taste of asparagus is mostly influenced by variety, soil type and growing conditions. In our experiment, ‘Vitalim’ hybrid exceeded by several sensory parameters. This hybrid scored 788.38 over 900. The elder population showed more interest in asparagus consumption than younger ones. 50% of the participants are able to pay only 500-600 HUF/kg. It could be realized to ensure the proper growing place, hybrid and direct trade. Marketing and more information is necessary to increase the popularity of this vegetable among the younger generation. -
Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids
39-45.Views:198Cotyledon protoplasts were isolated from 16-18-day-old in vitro grown seedlings of 9 Brazilian and 3 Hungarian pepper varieties and hybrids. Large numbers (average 9.59 X 106 protoplasts g 14 fresh weight) of highly viable (average 87.0%) protoplasts were released using a pectocellulolytic enzyme mixture. Protoplasts were cultured in K8p mediuni using an alginate disc embedding method. The osmotic pressure of the medium surrounding the alginate-embedded protoplasts was reduced by replenishing the liquid medium at K8p:K8 ratios of 1:0. 2:1, 1:1 in the first. second, and third week, respectively. Initial plating efficiency (IPE) average was 38.5% and after 21 days protoplasts reached microcolonies (15-20 cells) stages. Microcolonies were transferred after 3-4 weeks to a MS-based medium supplemented with 1.0 mg I-1 zeatin, 3.0% (w/v) sucrose, 0.24% (w/v) phytagel and pH 5.8, whereupon they formed callus. Final plating efficiency (FPE) average was 0.29% at a plating density of 1.0 x 105 protoplasts Protoplast-derived calli were cultured on a range of MS-based media supplemented with either BAP, IAA, TDZ; and zeatin. No morphogenic response was observed in any genotype investigated.
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Chemical characteristics of nectarine genitors used for breeding program in Romania
41-43.Views:125The geographic location of Bucharest area, the nature of its soil and its climate offer remarkably favourable conditions for nectarine growing, with juicy and well flavoured fruit, good for fresh consumption and processing.
The five years' investigation (1996-2000) has helped to identify some nectarine cultivars and hybrids (NJN 58, ARK 165, ARK 139, Morton, ARK 107, Romamer I, ARK 125, Firebrite) with exceptional fruit quality (dry matter content, titratable acidity, vitamin C content, overall sugar, and amount of pectic substances).
Dry matter content varied over the three years from 8.3 to 18.5% (NJN 68), meanwhile titratable acidity varied between 0.26 and 1.08% (Romamer I). Sugar / acidity ratio was within the limits of 8.6 and 36.5 (Nectared 7). Nectarines have high vitamin C content, over 10 mg/100g (Fairlane, NJN 67, Regina, Harko), glucides were found in Firebrite, Romamer I varieties and NJN 21, CIR1T127, HFSR3P4, HNA hybrids.
Some cultivars have been recommended for planting in this southern area of Romania (Crimsongold, ARK 125, Harko, Hardyred, ARK 134), others used as quality genitors (ARK 85, Nectared 7, NJN 21, ARK 21, ARK 134, Fantasia) in breeding projects.
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Evaluation of different sources of resistance for breeding powdery mildew resistant grapevine varieties
35-40.Views:188The appearance of resistance to powdery mildew was investigated on seedling progenies of interspecific crossings. Hybrids of American and East-Asian wild species and one Vitis vinifera variety (Janjal kara) were used as source of resistance in the crossings. The resistance of foliage and of berries were tested in the field at the time of vintage. The degree of resistance was ranked into six classes.
In American and Asian hybrid derivatives there were few highly resistant progenies, but in the hybrids of Janjal kara variety more than 90 % of the progenies proved to be highly resistant to powdery mildew. According to the distribution of classes of resistance in the populations the resistance of American and Asian sources are mostly influenced by additive gene affects. The resistance of Janjal kara seemed to be mono or digenic.
No close correlation was found between the powdery mildew resistance of the foliage and that of the berries.
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Two new registered table grape varieties
51-53.Views:145Two table grape varieties — Kósa and Narancsízű — were registered of the hybrids bred in the Research Institute for Viticulture and Enology in I lungary. Both varieties ripe early and are much in demand for the attractive clusters and flavours. As they are hybrids of Vitis vinifera they require similar growing conditions. In the same time with their qualification the two varieties are included in the variety list and allowed to be propagated.
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Fruit formation dynamics in parthenocarpic cucumber (Cucumis sativus L.) in spring forcing
111-114.Views:16820% of the cucumber crop of the world belongs to the parthenocarp type. Parthenocarp cucumber forcing has a great importance in Hungary, too. In our country the whole area of parthenocarp cucumber forcing was approximately 500 ha of the last years (2000-2002) and 75-85000 tons of yield has been harvested (MGYSZT, 2003). It means 15 — 17 kg/m2 as an average yield. In European forcing systems, parthenocarp cucumber is usually planted in January or February and it is harvested in spring or early summer. In Hungary cucumber forcing is the most profitable in two separate periods: spring and autumn, the reason for it is the changes of the average prices of fresh market cucumber, but spring forcing is still the most profitable. Forced cucumber cultivars are mostly parthenocarp; non-parthenocarp cultivars are grown in summer preferably. Cucumber cultivars, forced in our country, are hybrids, and 90% of them are offered by foreign seed companies (KristOfne, 1998.). The productivity of these hybrids is high and the productive period is quite short. All the mentioned details give the reason why it is important to know everything about the productivity, the dynamics of growth, and the possibilities of timing of parthenocarp cultivars, and it is also important to learn how to control all these parameters. Our spring cucumber-forcing experiment aimed to characterize of those parameters mentioned.
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Grape rootstock - scion interactions on shoot growth and cane maturing
30-34.Views:214Interaction between the rootstock and scion varieties (clones) was examined. Berlandieri x Riparia T. K. 5BB, Berlandieri x Riparia T.5C being the most widely used varieties in Hungary, Fercal, Ruggeri 140 being recently used on special soil conditions ; Georgikon 28 a new established variety and Berlandieri x Riparia T. 8B GK 10 clone were the rootstocks used. The V. vinifera L. varieties were a clone of Italian Riesling and two new hybrids from the same cross population f ( Noble Italian Riesling X Ezerjó) X ( Noble Italian Riesling X Pinot Gris ) }. This study presents the results how the growth habit depends on different scion/stock combinations in the first three years.
Shoot growth characters were observed during the initial years after plantation in the vineyard until we got the first yields. Our results did not agree with those of Pospisilova (1977) that the anticipated vigour of shoot growth will appear later in the vineyard only. However we agree with Zimmerman (1970), who showed that the differences are caused by the rootstocks in the growth habit of vines during the first two years already. The differences in shoot elongation decreased similarly to the rootstocks in the first year of fruit production. It shows us that the intense root development caused vigorous shoot growth, which we think to be a rootstock effect. When the vegetative and generative development are balanced already, the effect of scions in shoot elongation became stronger than the effect of rootstock.
We also found great differences in the maturation of wood each year from planting of the vineyard to the year of fruit production.
During the first years the differences of cane maturation were greater according to the scion varieties, the means were significantly different. In the first ripening year the differences dwindled or increased due to the rootstocks. This means that the effect of the rootstock on cane maturation became stronger in the first year of fruit production.
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Long term investigations of flowers and leaves on mainly non-domestica plums
73-79.Views:190The author dealt with plum species representing different eco-geographic areas by their genetic adaptation and their hybrids, as European (P. domestica, P. italica, P. cerasifera), Asian (P. salicina, P. simonii, P. ussuriensis), American (P. americana, P. besseyi, P. munsoniana, P. tomentosa). The rootstocks of the trees examined were seedlings of C. 679 myrobalan with the exception of Laroda and Santa Rosa II, which were grown on three different stocks: seedlings of C. 174 myrobalan, C. 449 bitter almond and C. 471 sweet almond. The size of peduncle, length of pistil, stamen number per flower, relative stamen number (SN/PL) have been suitable for description and distinction of varieties. Similarly shape of leaves, length of petiole, length and width of blade helped the identification.
The ratio of the dimensions of leaves, length of petiole and of leaf blade, also contributed to the distinction of European, Asian and American plum species, notwithstanding their relations with ecological conditions as well as historical, technical properties, pomological features, etc. Computed indicators (relative stamen number and shape-index of leaves) also have been useful data.
Significant correlations have been found between colour of nectaries and mean values of variety-groups. The potential values of non-European varieties for purposes of commercial production could be forecasted from the point of view of quality, ecological, pomological as well as market value. It is important, however, to know the effect of the rootstock and growing site as well as their interaction, on the one hand, whereas the resistance or tolerance of the varieties as limiting factors, at least to the sharka (Plum pox) virus, Xanthomonas pruni, on the other hand (cf. Surányi & Erdős, 2004a and 2004b).
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The effect of rootstock on the nectar production of apple cultivar `Idared'
15-25.Views:137Approaches based on the daily rhythm of apple flowers provide a new stage in nectar research, where the synchronous functioning of sexual organs is studied. In the flower biological studies the insect attraction of flowers was also studied. The two most important factors of insect attraction are the pollen- and nectar production of the flowers. From 1993 to 1998 we studied the food, that flower had to offer for the pollinating insects at different times of the day.
Studies were carried out on 'Idared', one of the hybrids of 'Jonathan' apple cultivar. The fruit of 'Idared' is bigger than the fruit of `Jonathan'. It is bright red, transportable, has a bigger productivity and is not subject to Jonathan-spots. Concerning its inner characteristics, it is juicy, the flesh consistency is better than that of 'Jonathan', but its acid/sugar ratio is worse (Sansavini et al. 1981).
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Epidemiological survey of Xanthomonas arboricola pv. juglandis and Gnomonia leptostyla on natural population of walnut (Juglans regia) in eastern Transylvania
7-9.Views:2140In Romania, walnut Juglans regia L. is an important fruit crop, although most of the fruit production comes from non-grafted walnut trees, which are natural hybrids. Breeding programs have been launched during last 30 years to develop new cultivars with uniform fruit quality. In addition, foreign cultivars have been introduced and tested to establish a valuable walnut genepool. To improve the present assortment of generative rootstocks in walnut and to examine the infestation level, a long term survey was carried out in Eastern Transylvania. The main physical characteristics of fruits and its variation to the infestation level were considered. The cumulative distribution of Xanthomonas arboricola pv. juglandis and Gnomonia leptostyla were relatively low and the maximum value was around 15%. The highest infestation of husk with X. arboricola pv. juglandis was observed for roundish forms and differences were statistically significant comparing with other phenotypes. Infestation with G. leptostyla was similar for roundish, elliptic and thwarting egg-shaped phenotypes, while the husk infestation for egg-shaped phenotypes was not observed. The walnut population studied in our experiment can be considered as a genetically valuable population. More than 20% of them have upper class quality fruits with at least or more than 50% of nutmeat. Do to the large scale climate variation in Eastern Transylvania and the high humidity favourable for pathogen infestations, these population can be considered resistant and well adapted to abiotic and biotic factors.
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Study of genetic resources for Powdery mildew resistance by biochemical and physiological parameters on peaches and nectarines in Romania
57-61.Views:144During 1997-2002, studies on the physiological and biochemical changes due to powdery mildew attack in peach and nectarine cultivars and hybrids belonging to different classes of resistance had been performed at the Research Station for Fruit Tree Growing (RSFTG) Baneasa Bucharest, located in the southern part of Romania. The study was based on disease assessments in the field and on other diagnosis criteria.
The southern part of Romania has favourable ecological conditions for growing peach and nectarine but trees have been attacked by various pathogens such as Sphaerotheca pannosa (Wallr.: Fr.) Lev. var. persicae Woronichin fungus for a long time. The presence of the pathogen causes important changes in the quantity and quality of fruits and affects the metabolism of trees.
This study was focused on several physiological and biochemical parameters such as photosynthesis and respiration intensity along with catalase activity; and changes in some biochemical compounds such as free, total and linked water content, dry matter, nitrogen and protein content, tannin levels. The aim was to find possible correlation among the above parameters and the biotic stress induced by the powdery mildew fungus calculated as attack degree (AD%).
Results showed that photosynthesis diminished significantly in the case of the attacked phenotypes, the respiration was more intense in the infected cultivars and catalase proved to be less intense in the affected plants. The total water content significantly increased in contrast to the dry matter and tannin contents, which greatly reduced in the case of resistant cultivars such as 'Victoria', Vectared 7', 'KB 11-40'. The total nitrogen and protein levels showed less influence on resistance to powdery mildew on all cultivars. All genetic material found resistant to powdery mildew is currently used in peach and nectarine breeding programs in Romania.
Further studies focused on isolation and characterization of resistance genes for powdery mildew resistance will be done in the future based on data collected during several years.
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Valuable offsprings of Seyve-Villard 12375
75-77.Views:209Powdery mildew, Phylloxera and downy mildew epidemics devastated large areas of vineyards in Hungary in the 1800s. To establish new plantages resistant so called "direct producers" were imported from America (direct means "without grafting"). Direct producers have secure yield but their quality is inferior. After World War II, an organized breeding programme was started to replace them and parallel to this the law prohibited their production. As a resistance gene source, the E.2 clone (S. V. 12375 E.2) of Seyve-Villard 12375, Franco-American hybrid selected in Eger was used by Hungarian breeders. Several valuable hybrid families have originated from crosses of this variety hybrid.
In the Research Institute for Viticulture and Enology at Kecskemét, two valuable table grape hybrids were registered under the names of R.65=Esther and R.78=Fanny coming from 2 hybrid families of the parent S. V. 12375 E.2. Both varieties may be cultivated "environmentally".
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Variability and differences of growth vigour in the set of 36 genotypes of apricot (Prunus armeniaca L.).
30-34.Views:145Growth vigour of 36 apricot cultivars and new hybrids grafted on apricot seedling rootstock (Prunus armeniaca L.) was evaluated on the base of measurements of stem girth from the 411' to the 10th year after planting. There were differences in growth vigour of genotypes under study. In the evaluated set of genotypes the control cultivar 'Veecor may be classified as a genotype with below-average growth vigour. Only four genotypes (-Reale d'Imola-, Sanagian -Moldavskii krupnoplodnyl and 'LE-2385') were found with significantly higher growth vigour than that of control cultivar 'Veecot' in years of the end of experimental period. Two genotypes (Farmingdale', -LE-SE0-24') were found with significantly higher growth vigour only at the beginning of experimental period and one cultivar ('Vivagold-) with significantly lower growth vigour in the first four years. Genotypes with different growth vigour can be used in further breeding programmes and/or as components inhibiting or supporting the growth in indirect vegetative propagation. Within the whole experimental period, the rank of growth vigour of genotypes practically did not change. This was demonstrated by highly significant or significant coefficients of correlation existing between individual pairs of years (r=0.32+ to r=0.96++). As far as the time difference between years in individual pairs of years was higher, the correlation coefficients were lower. In individual years, variability of growth vigour was relatively low and ranged from 9.83 to 13.64%.
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In vitro plant regeneration from immature embryo axis and cotyledons of common bean (Phaseolus vulgaris L.)
93-97.Views:139Phaseolus vulgaris L. is the most important economic species within the genus Phaseolus. It is grown in all parts of the world. Genetic improvement by conventional breeding has met considerable success, although production of hybrids between species within the genus has been limited due to sexual incompatibility. Recent advances in tissue culture have offered the opportunity to produce cultivars, which could not be obtained by conventional breeding methods. The use of tissue culture and genetic engineering is viewed as a logical approach to improve bean production. Gene transfer techniques will have a great impact on legumes. Although the concept of cell totipotency is widely proved, in vitro morphogenesis has not yet been achieved for a large number of cultivated beans. Regeneration protocols are strongly influenced by the genotype. In tissue and cell culture of beans, the factors controlling shoot morphogenesis and somatic embryogenesis are still unknown. The reported data suggest a possible way for future research.
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Reaction of different Capsicum genotypes to four viruses
61-64.Views:181The objective of this study was to examine the reaction of 44 Capsicum genotypes to common strain of Tobacco mosaic virus (TMV-C/U1), Obuda pepper virus (ObPV), NTN strain of Potato virus Y (PVYNTN) and legume strain of Cucumber mosaic virus (CMVU/246). Reaction (extreme resistance, hypersensitive reaction, latent susceptibility, susceptibility) of the tested Capsicum species/hybrids and breeding lines seemed to be greatly depending on hosts and viruses. Out of the breeding materials 4/99 F2 and IX-8 in to CMV-U/246, while 32.Bogyisz. type, VI-57 ii. 57/83 and V-12=19/98 to TMV-C/Ui showed extreme resistance. Two lines (V-25 F1=32/98 F1 and V-27 in F4=35/98 F4) showed hypersensitive reaction to ObPV. Latent susceptibility to PVYNTN was observed in case of all eleven tested Capsicum genotypes and in case of several lines to TMV-C/U1, ObPV and CMV-U/246. Other breeding materials proved susceptible to the mentioned viruses. Pepper genotypes showing extreme resistance and hypersensitivity could be used for resistance breeding to viruses.
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Phenological and fl ower morphological studies on different plum and prune cultivars
43-52.Views:25320–22 and 5–7 years of kind collection of 100 cultivars studied phenology and fl oral morphological basis of characteristics in 1994–1998 and 2009–2011. The varieties were collected 3–5 trees in the gene garden, all were on C. 359 myrobalan seedling. Detecting differences between the test cultivars are suitable for stamps, and also show that relatively short-term observations are useful for describing the comparison, to distinguish varieties. The taxonomic ordination of cultivars and groups of fruits suitable to distinguish colors shown that the fl owering and ripening time, the nature of the reproductive organs and even Sharka-rate sensitivity is associated with those groups. Differences between the age of trees mainly sexual organs and fertility were shown. Affecting rate of climatic effects was not as large as the first line of erratic rainfall, air temperature spikes or possible values would be expected. The results of the breed, a gene bank of perception, in particular, the localization of hybrids, clone-type variants are useful in the analysis. Among other things – this is due to late-maturing, relatively well-stored temporarily, and not-blue plums, Sharka-infected tolerant or resistant varieties selection (cf. SURÁNYI 2013a-c) and will be disseminate in Hungary.
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Growth and yield of sweet cherry trees on different roostocks
98-101.Views:243The first nine years' results of sweet cherry rootstock trial from Hungary are presented with the aim to select efficient rootstocks for the local ecological conditions. The trials were established in 1989 with 'Van' and 'Germersdorfi óriás' cultivars on the following rootstocks: mahaleb Sainte Lucie 64, Colt, MxM 14, MxM 97. All the trees were headed at 80 cm and trained to a modified Brunner-spindle system. Tree size and yield was measured every year, and the cumulative yield efficiency was calculated.
Based upon the results, mahaleb cherry SL 64 is a vigorous rootstock with good compatibility and productivity. In comparison to SL 64. the trunk cross-sectional area and canopy spread of the 'Van' trees decreased by 10-15% on rootstock Colt, while the trees of 'Germersdorfi orias' on Colt roostock grew even larger than on SL 64. Considerable 30-40 % reduction of tree size was achieved on trees grafted on M x M hybrids. Trees of `Germersdorfi óriás' showed a similar tendency without significant differences. The cumulative yield efficiency of `Van' trees after nine years was highest on rootstock MxM 14, followed by Sainte Lucie 64. Trees on Colt and MxM 97 rootstocks showed low productivity. `Germersdorfi óriás' produced the highest cumulative yield efficiency on SL 64, followed by MxM 14 and Colt and last MxM 97. Biennial bearing index of heavy cropping 'Van' trees was smaller than that of `Germersdorfi óriás'. The trees of 'Van' on MxM 97 showed higher biennial bearing index, while 'Germersdorfi orias' on different rootstocks showed similar tendency, but without significant differences.
MxM 14 and MxM 97 rootstocks reduced the crotch angle of the shoots on both sweet cherry varieties which is disadvantegous to spindle training. Slight suckering (1-3/year) of the rootstocks Colt, MxM 14 and MxM 97 were observed during the first few years.
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The importance of Hungarian melon (Cucumis melo L.) landraces, local types and old varieties (Review)
83-87.Views:172While supermarkets devote whole aisles to hybrids, traditional varieties are hard to find, and becoming scarcer day by day. Unfortunately, countless old melon varieties have already been lost. Luckily we succeded in collecting most of these varieties, and thus conserving them in Gene Banks. Landraces, local types, and old breed races show many characteristics that could be useful in organic farming. It is important to get acquainted with these varieties and cultivars, because they have greatly adapted to the climatic and pedological conditions of the Carpathian basin. Therefore their conservation is essential for the protection of Hungarian genetic variability. With the help of utilising our landraces in organic farming; careful selection; and the usage of marketing strategy in order to enhance quality features, such as unique flavour; we could reclaim the one-time excellent reputation of Hungarian melon.