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Agrobacterium transformation of Rhodiola sp.: current status and limitations
37-42.Views:356The study of secondary metabolites has led to the discovery of new drugs for treating human diseases. However, consistent plant supply can be challenging, leading to the use of plant tissue culture techniques such as hairy root culture. Hairy roots have stable genetics, lateral branching, and can produce secondary metabolites, including alkaloids, flavonoids, and terpenoids. Research on hairy roots as a subject began in the late 19th century, and for the last four decades, hairy roots have been utilized for producing secondary metabolites and recombinant proteins. This article focuses on Rhodiola species - genus of perennial plants that belongs to the family Crassulaceae - and its potential as a source of secondary metabolites using hairy root culture techniques. Rhodiola sp. is widely distributed throughout the Arctic regions of the Northern Hemisphere, with several species having significant medicinal properties. The article discusses the possible use of hairy root cultures for the production of Rhodiola secondary metabolites, including salidroside and rosavins, which have demonstrated significant pharmacological activity in various studies. The use of elicitation and genetic engineering techniques to boost secondary metabolite production in Rhodiola hairy roots is also explored. Overall, the article highlights the potential of Rhodiola hairy root cultures as a valuable source of secondary metabolites with medicinal properties. However, despite some studies Rhodiola hairy root induction and culturing still remains highly unexplored.
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Fungicide resistant Trichoderma strains causing compost infection in shiitake production
67-70.Views:242Trichoderma infection represents the major problem of shiitake production in the growing house of the Research Institute at Kecskemet. Heavy infections occur mainly on the compost before spawn run. All the isolated strain belongs to the species Trichoderma harzianurn, but morphology of the colony indicates that there are more strains liable for the infection. Source of infection remained unclear but the Trichoderma strains can be considered as weed moulds rather than true pathogens of shiitake. All the Trichoderma isolates showed unusual high degree of resistance to fungicides.
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Influence of environment in electro-chemical quality of tomato
17-20.Views:320The aim of the research was to investigate the impact of different cultivation environments of tomato cultivars, the electro-chemical and the qualitative traits of the tomato fruits. The quality of tomato fruit harvesting was evaluated at the Laboratory of Institute of Horticulture and Viticulture University of Natural Resources and Applied Life Sciences, Vienna. Two hybrids of tomatoes were tested Belle-F1 and AmaF1, in two locations and two different treatments (open field and plastic tunnels). Model of experimental fields was based on random method and included three replications. Following parameters were analyzed and tested: pH, Rh, P-Value, Nitrates and the content of vitamin C. The values of pH for treatment in the open field and indoor production system were not significant (4.47 to 5.05). For P-Value derived from the two treatments the maximal values were without significant differences, while minimal values were significant. Study has shown also that the highest values of nitrate content (16.34 mg, open field) compared to average value differences were + 0.384 mg or 43.83% higher. In indoor production the nitrate content was lower in compared with plants cultivated in open fields, so these differences were +3.81 mg or 26.4 0%, and highly significant for both levels. In relation to vitamin C content, the highest value was identified in the indoor systemof 298.6mg, with variations from the overall average of 16.43% or + 42.15 mg. The coefficient of variation values for both levels was of 29.96 respectively 27.31%.
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The effect of rootstock on the nectar production of apple cultivar `Idared'
15-25.Views:246Approaches based on the daily rhythm of apple flowers provide a new stage in nectar research, where the synchronous functioning of sexual organs is studied. In the flower biological studies the insect attraction of flowers was also studied. The two most important factors of insect attraction are the pollen- and nectar production of the flowers. From 1993 to 1998 we studied the food, that flower had to offer for the pollinating insects at different times of the day.
Studies were carried out on 'Idared', one of the hybrids of 'Jonathan' apple cultivar. The fruit of 'Idared' is bigger than the fruit of `Jonathan'. It is bright red, transportable, has a bigger productivity and is not subject to Jonathan-spots. Concerning its inner characteristics, it is juicy, the flesh consistency is better than that of 'Jonathan', but its acid/sugar ratio is worse (Sansavini et al. 1981).
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Differing effects of grass and polythene mulches on sweet potato productivity in derived savannah agro-ecology
24-30.Views:438Sweet potato as a food crop that is grown for both its tuberous roots and vegetative parts, the latter of which also serves as animal feed. Its production in the savanna regions can be limited by high evaporative demand, especially where soils with low water retentivity are involved. This work studied the effects of various locally available polythene as mulch materials for sweet potato growth in derived savanna agro-ecology. An experiment was thus conducted at Nsukka, a derived savannah in southeastern Nigeria, with orange fleshed variety of sweet potato. Treatments were polythene mulches of contrasting colours (black, white, red and blue polyethylene), including also organic mulch (dry grasses) as reference and a no-mulch control. The experiment was executed in two successive cropping seasons, and the data averaged and analysed. Agronomic and weed incidence data collection was done fortnightly from 2nd to 10th week of planting. The results showed that the polythene mulches (especially the black one) were better in the control of weeds. Furthermore, the sweet potato yields were affected significantly (p < 0.05) by mulching on the total number of tubers for each plant with grass-mulched plots showing the highest value (36) and transparent polyethylene-mulched plots the lowest (19). Also, total weight of tubers per plant at harvest differed (p < 0.05), with grass-mulched plots showing the highest value (2.39 kg), followed by the control (no mulching) plots (1.62 kg), then the coloured polyethylene mulched plots with the red polyethylene-mulched plots as the lowest (0.64 kg). This study recommends the use of organic mulch or no mulching in sweet potato production in this agro-ecological zone as they produced the best yields.
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Economic figures of apple production at national level of Hungary
103-105.Views:22733Apple is the most important fruit in Hungary, despite of that, it is a paradox that during the last years, apple growing was the most unsuccessful enterprise among the prosperous ones in the country. The real cause of regression was unequivocally the low price, which could be obtained and the inadequate structure of apple varieties. A heavy problem is still the overweight of the quality doomed to supplying the processing industry instead to the fresh consumption. In order to improve the conditions, we aimed the economic analysis of the five year long period, 2005-2009, to trace the changes involving values and costs of production, income and all conditions combined with this activity. The data presented in the paper are means of a heterogeneous population of values, which cannot be utilised for immediate use in management, but they may reveal the challenges and tendencies of that branch in economics.
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Study on the mushroom species Agaricus blazei (Murill)
45-48.Views:365Nowadays, Agaricus blazei is known all over the world as a mushroom species with unparalleled medicinal properties besides its culinary delights and therefore its regular consumption is recommended in the first place for the prevention of various cancers. For the past decade researchers have been active in the study of its therapeutic properties. Its production started only a decade ago and there are a number of questions yet to be answered. At the moment, the most widespread technique is to grow it using composts similar to those of the button mushroom. In contrast to the common button mushroom, its production differs markedly from that of the button mushroom in that it develops fruiting bodies only in light and requires that the compost temperature be high, around 25 °C, also in the fruiting period and has a much higher need for fresh air. It is also sold in fresh form but mainly as a dried product, in powdered form or as capsules or pills.
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Disease progress of apple scab caused by Venturia inaequalis in environmentally friendly growing systems
56-62.Views:557Progression of apple scab epidemic in six apple cultivars, including two current and susceptible (Gala Must, Elstar), two old (Egri Piros, Darusóvári), and two resistant cultivars (Relinda, Releika), were described and analysed in a two-year-study, in two environmental-friendly growing systems (organic and integrated). Curves of disease progress, linear regression analysis of transformed disease incidence data and Area Under Disease Progress Curves (AUDPC) were used to characterise the epidemic processes of the selected cultivars. Cumulative disease progress curves showed continuous but asymmetrical scab development on the moderate or highly susceptible cultivars Gala Must, Elstar and Egri Piros, and on the tolerant or resistant cultivar Darusóvári and Relinda, in both systems. The cultivar Releika showed no symptoms either on fruit or leaf. In linear regression analysis, the best linearisation was given by logistic transformation. Adequate parameters leaf disease incidence rate, of obtained from a regression equation, were higher in the organic system than in the integrated system. Values of AUDPC showed great differences in leaf disease incidences among cultivars and between growing systems. AUDPC gave more differences for comparison of progresses of disease epidemic than growth rate of disease in different systems of disease control. Moreover, the obtained results were compared with similar studies on different pathosystems, and biological interpretations of the analyses are discussed below.
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Evaluation of hot pepper (Capsicum annum L.) varieties for green pod yield and quality under rain fed production at Teppi, South Western Ethiopia
45-49.Views:398The trials were designed with three replications in a randomized complete block design in order to evaluate the phenological, growth and yield potential of hot pepper varieties such as ‘Mareko fana’, ‘Melka Zala’, ‘Melka Awaze’, ‘Melka Shote’ and local check. Significant difference was observed between growing year and used varieties. The varieties also performed significantly different (p<0.05) for most of the considered traits in the study. The result revealed that varieties ‘Melka Zala’, ‘Mareko fana’ and ‘Melka Awaze’ were scored highest green pod yield of 8.39, 8.71 and 11.39 ton per hectare, respectively. However, ‘Mareko fana’ variety was susceptible to disease attack as compared to other varieties. Therefore, promoting both ‘Melka Awaze’ and ‘Melka Zala’ varieties for widespread production for Teppi and the areas with similar agro-ecological conditions could contribute to boost the productivity of hot pepper. ‘Mareko fana’ could also be used for dry pod purpose due to its attractive color.
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Real-case application of mycorrhizal inoculums on Capsicum annuum L. var. longum cv. Szegedi and Kalocsai
75-79.Views:331The aim of this study was to test the use of commercially available arbuscular mycorrhiza (AM) inoculant Symbivit, a mixture of six species of Glomus spp., in spice pepper field cultivation. The inoculants containing arbuscular mycorrhizal fungi (AMF) was able to establish a symbiosis in the rhizosphere of pepper plants and mycorrhizal inoculation increased fresh and dry weights of shoots of spice pepper cv. Szegedi and only fresh weight of Kalocsai type. There were no significant differences in the root weights due to treatment only in fresh weight of Kalocsai pepper type. Treated plants of both variants exhibited an increase in cumulative crop production compared with control non-treated plants and the growth response of pepper was higher for var. Szegedi than var. Kalocsai. Mycorrhizal inoculation had a great positive effect on external hyphal length of AMF also showing differences in that between Kalocsai and Szegedi variants. The root colonization showed seasonality by treated and non-treated plants. The lowest degree of colonization was observed in June in general and colonization percent increased during vegetative development and there was a slight decrease at harvesting. In conclusion, it can be stated that inoculation with Symbivit containing mycorrhizal fungi could be an integral part of spice pepper production.
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Organic apple growing using sanitation treatments against apple scab
11-13.Views:543Effects or sanitation practices were evaluated on primary and autumn infection by Venturia inaequalis in an organic apple orchard at Eperjeske on the moderately scab-susceptible apple cultivar Jonathan in 2005 and 2006. Evaluated sanitation practices were: i) collection of fallen leaves in autumn; ii) destroying fallen leaves by disc cultivation in autumn; iii) spraying fallen leaves with 2% lime sulphur in autumn; iv) spraying fallen leaves with 2% lime sulphur in autumn and then collection of fallen leaves in autumn; and v) untreated control. In both years, most sanitation practices reduced significantly (P< 0.05) scab incidence in the primary infection periods compared to untreated control. The highest leaf scab incidence was observed in the untreated plots (26:2 and 24.3% in 2005 and 2006, respectively), while the lowest was in the treatments of spraying fallen leaves with 2% lime sulphur combined with collection of fallen leaves (11.7 and 12.3% in 2005 and 2006, respectively).
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Quality improvement in viticulture and winery
55-62.Views:229The author determines the development of the grape and wine production branches as a very cogent common task. Because of its importance this sector enables the livelihood of 100-150 thousand families and also of $ 80-100 million export income. A state subsidy is indispensable to the realization of that project. In the author's opinion on round 100 000 ha plantation area yearly 4-4.5 million hl wine can be produced, therefrom the export may amount to 1-1.3 million hl.
Yearly 3000 ha vineyards should be planted in order to maintain the present production capacity.
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Age-dependent physiological responses of Corchorus olitorius to aqueous extracts of Murraya koenigii and Tithonia rotundifolia
61-66.Views:85Sustainable crop production increasingly utilizes allelopathic plants as sources of bioactive compounds, yet their bimodal inhibitory–stimulatory effects and oxidative mechanisms require further elucidation. This study investigated the concentration-, tissue-, and stage-dependent effects of aqueous shoot extracts from Murraya koenigii and Tithonia rotundifolia on the germination, growth, and oxidative defense of Corchorus olitorius. Seed emergence and elongation of radicles and plumules were monitored in the laboratory using 50% and 100% aqueous extracts, while potted seedlings were treated separately with 100% extracts. Laboratory bioassays demonstrated a dose-dependent response: a 50% M. koenigii extract transiently increased germination by 4%, while higher concentrations of both species inhibited germination by 7–25%. Juvenile growth inhibition was tissue-specific, with 100% M. koenigii primarily suppressing plumule elongation and 100% T. rotundifolia significantly reducing radicle growth. Conversely, pot experiments using 100% extract concentrations of both plants significantly enhanced vegetative growth, physiological traits, and biochemical constituents, including protein and ascorbic acid. These extracts bolstered the antioxidant defense system—increasing superoxide dismutase, catalase, and peroxidase activities—while markedly reducing malondialdehyde levels. These findings provide evidence of allelopathic hormesis, where initial inhibitory effects transition into growth stimulation and oxidative stress mitigation during later developmental stages. The results suggest that M. koenigii and T. rotundifolia shoot extracts serve as effective eco-friendly biostimulants that improve crop performance by modulating antioxidant responses.
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Organic and mineral fertilizer effects on the yield and mineral contents of carrot (Daucus carota)
69-74.Views:747A greenhouse pot experiment was conducted to investigate the effects of ammonium-nitrate, food waste compost, bacterial fertilizer (EM-1) and their combinations on production and nutrient contents of carrot. The study was conducted on a calcareous chernozem and acidic sandy soils in a randomized complete block design with 8 treatments and four replications. NH4NO3 in chernozem soil increased the weight of carrot leaves only, while in sandy soil resulted in reduced yield and highly increased NO3-N content of roots. Sandy soil showed higher response of biomass production to food waste compost application than chernozem soil. The highest carotenoid content of roots was measured with compost treatment. Combined application of compost and NH4NO3 in chernozem proved to be good combination but in sandy soil have turn out to be less favourable than sole compost treatment. Bacterial fertilizer (EM-1) did not cause marked effect on the yield parameters, but caused increased phosphorus content of plant. In chernozem soil the maximum yield parameters were achieved with the combined treatment of ammonium-nitrate+compost+EM-1. In sandy soil the most favourable treatment proved to be the compost treatment. Results suggest that application of food waste compost as a nutrient source could be a promising agrochemical practice especially in soils having low organic material and low nutrient supply.
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Correlation of precipitation distribution and quality sweet cherry production
39-43.Views:842Sweet and sour cherry need 550–600 mm yearly precipitation. The critical period is 1–1.5 month after flowering, it is normally between 15.April – 15. June in Hungary. The rain induced fruit cracking is also a critical and costly problem for cherry growers. Fruits grown under arid conditions are less resistant against rainfall during harvest and up to 50–60% crack damage may occur. A computer program was developed to calculate the precipitation related production risks of sweet cherry. Focus of the research was Zala county. Spatial distribution of precipitation was compared in two directions (East and North of Zala county) based on the data of meteorological stations. The first results indicate that the developed method estimates the risks quite well, compared to the farm experiment results. The developed computer program can be parameterised according to the user’s requirements, this allows to take into account the real variety structure of a given orchard.
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Blumeriella jaapii (Rehm) v. (Arx) infection of some sweet cherry cultivars in two years with different precipitation conditions
37-39.Views:308We examined Blumeriella jaapii (Rehm) v. (Arx) infection on 21 sweet cherry cultivars in the cultivar-collection of the Experimental Station Pallag of the University of Debrecen, in 2004 and 2005. Considerable differences were detected in the infection degree of the different cultivars. In 2005, due to the more humid weather, the average infection highly exceeded data of the previous year. No symptomless cultivars were detected in either year. The least sensitive ones were Linda and the cultivar 11/106. The most sensitive cultivars were Early Müncheberg, Biggareau burlat and Round Solymári. Infection degrees of Alex, Rita and Vega were different from those of other cultivars on the basis of the two experimental years.
It is important to consider both the specific weather conditions of the year and cultivar resistance at planning the plant protection system. It is recommended for each cultivar to perform a fungicide-spaying after the harvest.
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Detergent induced pollen sterility in some vegetable crops
85-88.Views:275Efficacy of a popular synthetic detergent, Surf excel in some important vegetable crops viz. Okra or lady finger (Abelmoschus esculentus L.), chilli or red pepper (Capsicum annuum L.) and tomato (Lycopersicon esculentum Mill) was evaluated for inducing male sterility and hybrid seed production. Foliar sprays with aqueous solutions of Surf excel (1.0 and 1.5% w/v) in these crops induced complete pollen sterility. The treated plants showed a delay in flowering, a reduction in the number of flowers and fruits/plant, number of seeds/fruit resulting in a reduction in yield/plant. However, the male sterility thus induced was successfully exploited for hybrid seed production.
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Effects of integrated and ecological growing technologies on the growth and development of fruiting structures in new apple plantations
31-33.Views:372On the Horticultural Station of the Debrecen University, Pallag, two year old apple plantations of two varieties (‘Pinova’, ‘Golden Reinders’) have been studied, in autumn 2011. The growth of the cross section area of the trunk and the central axis were measured and compared to evaluate the effect of two alternative growing technologies: integrated and/or ecological. ‘Pinova’ proved to grow more intensely than ‘Golden Reinders’ independently from the growing technology. The effect of the technology, however, was expressed in ‘Pinova’, where the integrated technology produced more vigorous growth than the ecological one. The effect of varieties on growing intensity was more distinct than the effect of growing technologies. Under the ecological growing system, the difference between the varieties studied is more accentuated regarding growing intensity. By that reason, in ecological growing plantations of varieties with moderate vigour ought to be stimulated by all possible techniques (as pruning, nutrition etc.).
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A review of the orchard management in organic fruit production
43-49.Views:710The elements of orchard management have a determining role in the success of organic fruit production, therefore, it is essential that they are applied properly. The present review gives describes some aspects of the orchard management such as site selection, orchard soil, rootstocks and cultivars, planting materials and plant density, crown shape. Then, one of the most important elements, pruning is discussed with special emphasis on winter pruning, condition and apical dominance, degree and method of pruning, summer pruning. After pruning, an overview of fruit thinning, irrigation, soil cultivation, soil protection (cover plants and mulching) and fertilization is given.
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Macronutrient accumulation in green pepper (Capsicum annuum L.) as affected by different production technologies
13-19.Views:564Based on the experiments, an assessment was made to determine for green pepper the amount of nutrients extracted by unit weight of fruit and plant parts not meant to be consumed (foliage, stem, root), i.e. the specific nutrient requirements of pepper. A further objective was to find out to what extent nutrient accumulation in individual plant organs was influenced by differences in production technology and soil conditions.
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The Role of Human Factor in the Innovation of Apricot Production-Empirical Study in a Disadvantage Area of East-Central European Country
131-134.Views:1214Hungary is a traditional fruit growing country for ages. As fruit sector has a very high hand work request and value added, it has an important role to decrease the elimination of unemployment and the lack of income in the disadvantage rural areas. The study was made in the year of 2009, the studied population consisted of the members of the fruit-grower marketing organization (Gyümölcsért Ltd.), that organizes growing and sales of stone fruits in Hungary. The number of studied population were 95 capita, the number of fi lled out and evaluated questionnaires was 35. By the composition of the questions both qualitative and quantitative methods have been used. We tried to get answers to the following questions: Are the studied human factors (age, educational level, sex etc.) of growers, determined the extension of innovation of apricot production?
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The vegetable production programme
45-54.Views:293The authors' collective when drawing up the development of the vegetable branch emphasizes the supposition that
- in the future the domestic products will be overwhelming on the home market,
- a significant quantity of commodities will be exported into neighbouring countries,
- we may be continuously present at the EU market.
All these conditions can be based only on strong state assistance.
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Book review
37-39.Views:388Plum is a significant temperate fruit and a very important fruit species in Hungary as well. Cultivation has moved beyond the area boundaries of the Northern Hemisphere many centuries ago. Domestic (European) plum production has been particularly affected by the pandemic-scale destruction of the Sharka virus and worldwide breed changes. According to FAOSTAT (2016) data, world plum production is 12 million tones, with 36% from Eurasian, 63% from Japan and other Asian varieties. The share of American plums is only 1%. Domestication and dissemination of plums is „multi-stepped” because homemade (taste) plums are hybrids of two nature species in the first place, but Japanese plums (hybrids with Chinese plums or Prunus cerasifera) are not uniform; the role of the American plum species is much smaller, though their prospects cannot to predict with certainly. The book consists of 19 chapters, finding a complex way of summing up linguistic, historical, floristic, historical-botanical, cultivation, and morphological and anatomical knowledge.
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Extreme weather events inWest Hungary
29-32.Views:1034Agricultural production is a rather risky activity, as it is largely exposed to extreme weather events. This paper focuses on the frost and hail data in springtime, affecting fruit production in West Hungary. Regarding absolute minimum temperatures in April, significant increase of the standard deviations could be observed. Among the sites examined Nagykanizsa shows the highest risk values. According to our evaluation Nagykanizsa is not recommended to plant sour cherry, sweet cherry and apricot orchards because in some years spring frost will occur. If the plantation is in Zalaegerszeg, Szombathely, Gyôr or Keszthely than usage of frost prevention system is recommended. Siófok is the only area to grow apricot with good result.
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Evaluation of vermicompost application and stress of dehydration on mullein medicinal plants
69-77.Views:363The use of organic fertilizers is one of the suitable solutions in the organic production of medicinal plants due to its good effect in improving soil properties, reducing environmental effects, and better plant growth. To investigate the effect of vermicompost organic fertilizer application and water stress on some morphological and physiological traits of the Mullein medicinal plant, research was conducted at Isfahan Azad University in the form of split plots in the form of a randomized complete block design with 4 replications. The test factors included the application of vermicompost organic fertilizer at three levels of 0, 4, and 8 kg per square meter of soil and water stress at two levels of normal irrigation and irrigation at the time of 50% of the soil's agricultural capacity as the main treatment in research farm conditions. The results of the experiment showed that the application of vermicompost organic fertilizer and water stress improved the morphological and physiological characteristics of the Mullein medicinal plant compared to the control, i.e. no application of organic fertilizers. The results showed that the highest number of secondary branches, number of flowers of the secondary stem, diameter of flowering stem, the diameter of flower, and fresh weight of shoot in the Mullein medicinal plant were obtained by applying vermicompost organic fertilizer at the rate of 4 and 8 kg of soil. The application of vermicompost organic fertilizer at the rate of 8 kg in the soil increased flavonoids compared to the non-use of vermicompost treatment. However, to improve the vegetative growth and increase the reproductive efficiency of the Mullein plant and reduce production costs, the use of vermicompost organic fertilizer is recommended at the rate of 4 and 8 kg of soil, respectively.