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Examinations of 600-year-old seeds by means of archaeobotanical and genetical methods
79-80.Views:162About 600-year-old plant seeds were discovered in a well of a mediaeval cellar in the course of an excavation in Budapest. After the archaeobotanic purification seed of 16 species were found in large quantities. Seeds preserved in the best state were selected from each group. The existence of endosperm was analysed in these subfossils, which turned to be successful mainly in the case of grapes (Vitis vinifera) and cornels (Cornus mas). Seeds of these two species contained the most endosperm and remains of the embryo. DNA was extracted with the help of DNEasy Plant Mini Kit and analysed by RAPD-PCR method. The amplification of DNA extracted from cornel seeds resulted in detecting a 1500 by fragment, which makes the comparison of these samples with present-day cornels possible.
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Quality improvement in viticulture and winery
55-62.Views:140The author determines the development of the grape and wine production branches as a very cogent common task. Because of its importance this sector enables the livelihood of 100-150 thousand families and also of $ 80-100 million export income. A state subsidy is indispensable to the realization of that project. In the author's opinion on round 100 000 ha plantation area yearly 4-4.5 million hl wine can be produced, therefrom the export may amount to 1-1.3 million hl.
Yearly 3000 ha vineyards should be planted in order to maintain the present production capacity.
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The evaluation of grape vine decline pathogens in the experimental field of the Georgikon Faculty of Agriculture in Cserszegtomaj
19-22.Views:192Vine decline causes important economic loss in viticulture, especially in longer view. Several causal pathogen were described lately, however little is known about the etiology or epidemiology of these pathogens on grapevine rootstock. It is well known that these diseases affect grafted and rooted grapevines and are not specific to any scion-rootstock combinations. Our aim was to determine what pathogens are presents in the experimental field, especially causal agents of the rootstock decline. Different grapevine rootstocks and scion varieties were tested in our trial. Isolations were made from the wood tissue and pathogenity tests were done with isolated Cylindrocarpon destructans. The possibility of infection during the propagation process was studied as well. Most commonly Cylindrocarpon sp. and Phomopsis sp. species were identified from the examined varieties. Cylindrocarpon destructans was able to spread to apical (shoot) and basal (root) direction from the point of infection with uneven speed. Callus development is not inhibited by the fungi causing the leaf symptom of the vine decline. Shoot development is reduced if unhealthy parts are grafted to each other.
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Perspectives and tasks in horticultural production
11-22.Views:219The work summarizes the prospective conceptions of all the five horticultural branches. These branches (vegetable, fruit, grape and wine, herb and ornamental plant production) with the production of propagating material together amount to round 30-35% of the total value of the entire plant production. The performance of horticultural branches declined significantly because of privatisation and lack of capital. The accession to the EU urges the development of modernization and competitiveness, therefore the state subsidies are indispensable.
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Study on the yeast and mould biota of the botrytized grapes in Tokaj region in two years
61-65.Views:181The famous wine Tokaji Aszú is produced from "noble rotted" grapes infected and modified by Botrytis cinerea under special condition. The objective of this study was quantitative and qualitative characterisation of saprophytic fungi present on the surface of aszúberries, with special regard to yeasts. There were considerable differences in these populations depending on the origin of noble rotted berries, notably between berries taken from the vine or the ones taken from the winery. Beside Botrytis, other mould species like Penicillium, Aspergillus were commonly found, in widely varying population. Yeast counts were detected between 104 and 107 cfu/g berry. In the samples taken from the vineyard Candida pulcherrima predominated followed by some aerobic basidiomycetous species, but Hanseniaspora species were also present in relatively high population. After transport and storage of the grape in the wineries, population of aerobic yeasts and C. pulcherrima quickly declined and Candida stellata followed by other sugar tolerant species became dominant. This autoselection process directs attention to the importance of storage conditions.
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Conventional PCR primers for the detection of grapevine pathogens disseminated by propagating material
69-80.Views:304Polymerase chain reaction driven by sequence specific primers has become the most widely used diagnostic method to detect and identify plant pathogens. The sensitive and cost-effective pathogen detection is exceptionally important in the production of propagating material. In this paper we have collected primer sequence data from the literature for the detection of the most important grapevine pathogens disseminated by propagating stocks by conventional polymerase chain reaction. Basic protocols to obtain template nucleic acids have also been briefly rewieved.
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Effect of water supply on nutrient transport in grapevine varieties
64-68.Views:148The effect of water supply on availability of macro nutrient elements (N, P, K) by the plants in the soil and their transport in the plants were examined. In a field experiment two grapevine varieties characterized by higher (White Riesling B 7) and lower (Kövidinka K 8) water requirement were compared on the basis of N, P, K concentrations of leaf blades, petioles and berries. A different water supply of the vineyard was achived by striped coverage of the soil with plastic foil to exclude rainfalls from the beginning of May to ripening. Humidity of the soil decreased as the vegetative phase advanced. Soil cover resulted in 25-30% decrease of the water content in the soil at flowering but this difference gradually disappeared till veraison (i.e. the start of intense growth of the berries). The water consumption in the White Riesling B 7 plantation was more intensive. Mobility and availability of N, P, K in the soil was restricted by water exclusion (i.e. plastic soil cover) at flowering. Nitrogen was slightly affected, whereas P and K were in a higher extent. Comparing the transport of nutrient elements in the two varieties, leaf blades of Kövidinka K 8 contained less N and more P and K than White Riesling B 7 at flowering and more N and P and less K at ripening. Water deficiency inhibited K accumulation in the berries of White Riesling B 7, while this effect did not appear in Kövidinka K 8.Water exclusion decreased the yield of White Riesling B 7 already at lower bud loading, the yield of Kövidinka K 8 was affected only at higher bud loading. The higher yields of the treatments in Kövidinka K 8 plantation support the superior performance of this variety under the hot and dry climate of the Hungarian Great Plain.
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Magyar alanynemesítés története és eredményei
13-17.Views:197A filoxéra (Dactulosphaira vitifoliae (Fitch) európai megjelenése (1860) és pusztítása után több védekezési mód kipróbálása után az oltványkészítés vált a védekezés leghatékonyabb módszerévé. Ettő1 az időtől kezdve az alanyfajták használata a kötött talajú szőlőkben nélkülözhetetlenné vált.
Az alanyfajták használata es nemesítése az 1800-as évek végen Franciaországban (Millardet, Gaset, Courdec, Ganzin Pougnet, stb.), majd Olaszországban (Ruggeri, Paulsen stb.) kezdődött el. A múlt évszázad végen kezdődött hazánkban az alanynemesítés. Több szakember foglalkozott új alanyfajták előállításával. Ezek között Teleki Zsigmond pécsi szőlősgazda munkássága emelkedik ki, aki olyan alanyfajtákat állított elő, mint a Teleki 5C, a Teleki-Kober 5BB, a Teleki-Kober 125AA, a Teleki-Fuhr S.0.4 es a Teleki 10A. A Teleki hibridek a világ szőlőtermesztő országainak nagy részében megtálalhatók, előállításuk után száz év múlva világfajtákká váltak.
Az 1970-es években Keszthelyen az Agrártudományi Egyetem Kertészeti Tanszékén Bakonyi Károly es munkatársai foglalkoznak új alanyfajta előállításával es szelektálásával. Munkájuk során több értékes klónt és új fajtát állítottak elő. A hibridek közül a Georgikon 28 alanyfajta erős növekedésével, nagy szárazság- es mésztűrésével tűnik ki. Magyarországon szabadalmi oltalomba részesítették es elkezdődött a felhasználása. Franciaországban a Richter cég szaporítja. Németországban a Geisenheimi Szőlészeti Kutató Intézet 2002-ben országos
kísérletbe állította. Keszthelyen az alanynemesítést tovább folytatják, számos kiváló tulajdonsággal rendelkező hibridet értékelnek, amelyek közül néhányat (Georgikon 103, a Georgikon 59-et stb.) fajtaminősítésre bejelentenek.
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Polyphenol- and anthocyanin content changes effected by different fermentation- pressing and aging technologies
65-67.Views:209Different grape processing, fermentation and aging technologies were compared in our study on the white wine-grape variety Grüner Veltliner between 2012 and 2014 in Hungary,Cserszegtomaj. The vines are grown on brown forest soil on dolomite bedrock, stocks were planted 3x1 m row and vine space, respectively in our experimental area. The soil has slightly alkaline pH, the orientation of the vine rows are East-West. The training system is modified Guyot cordon, with 1 m trunk height and cane pruning method. After the harvest half of the yield has been put into the de-stemmer crusher before pressing while the other half has been pressed immediately (whole bunches). From the filtered and bottled wine anthocyanin, and polyphenol content was measured in 2013 and 2014. Another enological technology testing experiment has been set on aging of Grüner Veltliner in 2013. The wine was fermented with addition of fine lees from juice sedimentation. Traditional (racking only), battonage and fast ready-made aging technologies have been set together, each treatment in three replicates were observed.
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Comparison of the biogenic amine content of traditional and bio-wines
115-118.Views:149There has been a growing tendency in organic farming in recent years, in which organic grape growing and the production of biowine are also significant. The literature on bio-wine is rather limited, that is why we consider it really important to contribute to the data. As a result of the special conditions in organic farming we can expect bio-wines to have a different composition of nitrogen compounds from that of traditional wines. Biogenic amines could thus specially characterize bio-wines. In our research we compared the biogenic amine content of wines produced using organic methods and of those where traditional procedures were used. Our measurements have proved that it is not possible to clearly distinguish bio-wines from wines produced with traditional methods on the basis of amine content.