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Yielding capacity and regeneration of different ages fruit-bearing shoots in sour cherry
55-57.Views:187The yielding potential and regeneration (i.e. germination of hidden buds) after pruning of the fruit-bearing shoots of different ages were compared in sour cherry cultivars pruned to spindle shape. The examinations were carried out in order to determine the optimal pruning strategy resulting in the highest long-term yielding of sour cherry cultivars with intensive crown shape (free and thin spindle shape). According to our results, there are great differences among the studied self-fertile sour cherry cultivars in yielding and regeneration, which are worthwhile to be considered in determining the method and degree of pruning.
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Tree growth and inner characteristics of fruits in pear cultivars
29-32.Views:135Summary The aim of study was to investigate growth (crown growth and trunk diameter) and fruit characteristics [dry matter content (%), pectin (%), total acid (%) contents] of 10 pear cultivars ('Móri császár', 'Nyári Kálmán', 'Mogyoródi óriás', 'Fehérvári körte', 'Szegfa körte', 'Piroska', 'Mézes körte'). The crown development of the cv. 'Fehérvári körte' can be regarded as outstanding among the examined cultivars. The growth of the cv. 'Mogyoródi óriás' was low, this cultivar showed the poorest growth vigour. The width of the crown in several cultivars developed at a similar rate as the height of the crown. Outstanding values were shown by cvs. 'Fehervári körte' and 'Móri császár'. The development of trunk diameter was the highest for cvs. 'Piroska' and 'Hóka', and large growth of trunk diameter can be seen on cv. 'Fehervári körte'. Cvs. 'Mogyoródi óriás' and 'Mezes körte'showed weak growth. Outstanding dry matter content of fruits was measured on cvs. 'Fehervári körte' and 'Mézes körte'. The total acid content of fruits of cvs. 'Mézes körte' and 'Fehérvári körte'' was significantly different from the total acid content of cv. 'Mogyoródi óriás'. Pectin content was low in fruits of cvs. 'Mogyoródi óriás' and 'Fehérvári körte', while cv. 'Mézes körte' contained significantly more pectin. Vitamin C content we found was rather high in cvs. 'Mézes körte' and 'Fehérvári körte'.
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The impact of irrigation on the yield and tuber fraction distribution of potato cultivars
65-70.Views:132Potato is one of the most important plants in human nutrition, it is grown in about 140 countries. As a food, it can be prepared in many ways, it is easily digestable with proteins of high biological value, favourable dietetical-physiological characteristics, high starch content and good taste. An increase in its consumption would be favourable from the dietetical point of view. In Hungary, the growing area of potato has decreased considerably for the last decades and we are lagging behind the Western-European countries regarding yield. The experiments were carried out at the Experimental Site of the University of Debrecen at Látókép between 2002 and 2004. In the large-plot experiment, the yields and the distribution of the tuber fractions were studied for 9 medium early cultivars. The experiment was set up on 50 m2 plots on calcareous chernozem soil. Among the studied cultivars, 3 were of Dutch (Desiree, Kondor, Kuroda) and 6 were of Hungarian breeding ('Góliát', 'Hópehely', 'Kankan', 'Lilla', 'Százszorszép', and 'White Lady'). The experiment was set up in 4 repetitions in a randomized design, two of the repetitions were irrigated while two were non-irrigated.
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Old Hungarian grapevine cultivars and their relations characterized with microsatellite markers
27-31.Views:234Thirty-one old Hungarian grapevine (Vitis vinifera L.) cultivars were investigated on 7 microsatellite loci to characterize them, to separate the cultivars from synonym names, and to confirm parent-offspring connections. Conculta (group of cultivars or bud sports) members, such as `Goher' and Tajor' representatives, were studied to find a suitable locus for the separation. Synonyms, conculta members, subcultivars and clones of Turmine, which was the most important cultivar of Tokaj, were also analyzed to separate the members of the different taxonomic levels. Pedigree of 'Kiralyleanyka' was examined to find the missing ancestor, because the parent-offspring connection between the natural hybrid and `Koverszolo' is questionable.
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Fruit formation dynamics in parthenocarpic cucumber (Cucumis sativus L.) in spring forcing
111-114.Views:16820% of the cucumber crop of the world belongs to the parthenocarp type. Parthenocarp cucumber forcing has a great importance in Hungary, too. In our country the whole area of parthenocarp cucumber forcing was approximately 500 ha of the last years (2000-2002) and 75-85000 tons of yield has been harvested (MGYSZT, 2003). It means 15 — 17 kg/m2 as an average yield. In European forcing systems, parthenocarp cucumber is usually planted in January or February and it is harvested in spring or early summer. In Hungary cucumber forcing is the most profitable in two separate periods: spring and autumn, the reason for it is the changes of the average prices of fresh market cucumber, but spring forcing is still the most profitable. Forced cucumber cultivars are mostly parthenocarp; non-parthenocarp cultivars are grown in summer preferably. Cucumber cultivars, forced in our country, are hybrids, and 90% of them are offered by foreign seed companies (KristOfne, 1998.). The productivity of these hybrids is high and the productive period is quite short. All the mentioned details give the reason why it is important to know everything about the productivity, the dynamics of growth, and the possibilities of timing of parthenocarp cultivars, and it is also important to learn how to control all these parameters. Our spring cucumber-forcing experiment aimed to characterize of those parameters mentioned.
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Influence of strawberry and raspberry consumption on the antioxidant status of human body
109-113.Views:169Several parameters influencing the antioxidant capacity were measured in our experiments to compare strawberry and raspberry cultivars. The analysed cultivars were: 'Honeoye', 'Elsanta', 'Onebor (strawberries) and 'Glen Ample', Fertődi zamatos', 'Mailing Expoit' (raspberries). Besides the determination of dry matter content the content of glucose, fructose, total phenol and anthocyanine, the reducing power, H-donating activity and total scavenger capacity were measured as well as the compound of mineral nutrients. Among the species and the cultivars, differences were obtained. These results call the attention to the features of cultivars and to count on the antioxidant-capacity describing values in case of consuming these fruits. In early spring and summer the consumption of strawberry and raspberry has a beneficial contribution to the antioxidant status of our body. In this respect these are more valuable than the year-long consumable apple. Accordingly, our measurements showed higher amount of sucrose, phenolic compounds and anthocyanine in raspberries, but strawberries contained more glucose and fructose and had stronger H-donating activity. It is worth to keep in mind that e.g. which cultivar contains more of certain compounds because of the strictly determined allowable dose in some diseases (e.g. diabetes).
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Effect of intensity of bee visitation and the foraging behaviour of honeybees on the fruit set and yield of apple (Malus domestica Borkh.)
31-39.Views:155Based on the results of our experiments, both the relative and the effective intensity of bee visitation were rather different depending on the cultivars as well as the time of the day. While it varied between relatively extreme values (40-80%) in the morning for the varieties examined, there were much smaller differences between the intensity of bee visitation at the afternoon, because the relative bee visitation attained 70-90% at each cultivar. These results showed that the differences arising from intensity of bee visitation of different cultivars should be taken into consideration more carefully in the morning in orchard planning and in estimating the number of honeybee colonies required.
The results showed that the greatest percentage of fruit set and the highest number of viable seeds per fruit were measured on branches of those cultivars that were most frequently visited by pure pollen gatherer bees as well as by bees collecting both nectar and pollen (mixed behaviour). The effect of pure pollen gatherers and of bees with mixed behaviour was highly significant from the statistical point of view on the fruit set and the number of viable seeds per fruit.
Those bees that were sucking nectar only from apple flowers did not proved to be effective pollinators at all. Relationship between their number and the fruit set as well as the number of viable seed per fruit were not significant because the coefficient of correlation was close to nil.
The ratio of side worker nectar gatherers was negatively correlated with the fruit set and the seed content of fruits of apple cultivars examined at both of our experimental sites, at Mosonmagyaróvár and Feketeerdő as well. The presence of side worker nectar gatherers resulted in higher decrease of fruit set and seed content of fruits at Feketeerd6 than at Mosonmagyaróvár, especially in the morning.
The effect of flower visiting intensity by other pollinating insects was found to be fairly variable according to the time of the day. In the morning they had no effect on the fruit set as well as on the seed content of fruits either at Mosonmagyaróvár or at Feketeerdo. However, at the afternoon, when their intensity was greater, the correlation was a bit stronger.
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Effect of postharvest sodium benzoate treatment on some fruit parameters of two organic apple cultivars
35-37.Views:444The aim of this study was to investigate the effect of postharvest sodium-benzoate treatment on some fruit parameters of two organic apple cultivars (’Topaz’ and ’Florina’). Assessments were made at three times during storage: 17 November 2019, 20 December 2019 and 23 January, 2020. During every assessment dates, each fruit was observed separately, and determined the proportion of i) healthy fruits (%), ii) post-harvest fruit rot diseases iii) fruits with mechanical injury (%), iv) fruit with russetting (%), v) damage of codling moth and vi) Ca-deficiency symptoms. In addition, fruit weight (g) was measured at each assessment date. Sodium benzoate reduced the fruit decay and the proportion of healthy fruit was higher in this treatment compared to water treated fruit. This effect could be seen in all assessment dates and on both cultivars. Effects on other parameters were various according to cultivar and assessment dates. Loss of fruit weight was similar in both treatments and cultivars compared to control treatment.
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Consumer preference for apples: the role of attributes influencing the choice and consumption
37-43.Views:375Consumers consider good quality fruits to be those that look good, are firm and offer good flavour and nutritive value. Nowadays, consumers are, however, increasingly interested in food qualities which cannot be discovered by looking, tasting or smelling the products but their roles are not yet really cleared up. Therefore the objective of this study was to explore the importance of selected kinds of attributes (taste, size, colour, cultivar, origin and price) in fluencing the choice of apples of customers. In accordance with several authors, fruit qualities (taste, size and colour) seemed to be the major attributics influencing the choice of apple independently of people's age and gender. However, the size of fruit had lower importance with increased age. Generally, females gave higher importance rating for most attributes than did male. The price was getting more important for consumer's choice with age which can be connected with their socioeconomic situation. The origin of fruit and the cultivar did not have important influence on consumer's choice. Authors also investigated the preference of consumers for six selected apple varieties ('Jonagold', 'ldared', 'Royal Gala', 'Golden Reinders', 'Braeburn' and 'Granny Smith'). and pointed out the role of the origin in fruit quality and in choice of apple in the case of 'Granny Smith' cultivar. Apple fruit samples from Austria, Argentina, Chile, Hungary and South Africa were involved in this study. Authors evaluated fruit quality parameters of above cultivars and compared them to consumer preference. Consumer preference usually represented the quality attributes of the fruit well. It is also concluded that degree of liking of apple cultivars varies through gender and age. Children and young consumers preferred 'Royal Gala', 'Granny Smith and 'Braeburn' mostly. In spite of 'Idared' is one of the cultivars grown on the largest area in Hungary, the lowest preference ratings were given for it in both gender categories. Middle-aged consumers (between 25 and 50 years or age) preferred crispy apples with red or blemished skin color ('Royal Gala' and 'Jonagold'). 'Jonagold' and 'Idared' were the most preferred cultivars for the consumers above the age of 50 likely because of their relatively low price, as price plays a significant influencing role in the purchase of these consumers. The relatively expensive and soury 'Granny Smith' appeared not really preferred by this age group. In spite of the significant differences in instrumentally measured fruit quality parameters among 'Granny Smith' fruit samples from different countries, consumers did not give significantly different preference rating scores for those.
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Effect of acidity on growth rate and stroma formation of Monilia fructigena and M. polystroma isolates
63-67.Views:197The effect of acidity (pH) ranges on the mycelial growth and stroma formation of Monilia fructigena Pers: Fr. and of M. polystroma van Leeuwen was determined on agar plates and apple fruits. Four isolates of each of the brown rot fungi and two apple cultivars, `James Grieve' and 'Cox's Orange Pippin', were used for the study. For the agar plate study, a range of the initial pH was prepared from 2.5 to 6.5. The dishes were inoculated with a 4 mm plug of each isolate and incubated at 23 °C in darkness. The mycelial growth was measured after 1.5, 4, 7, 10 and 20 days of incubation. After a 30-day incubation, stroma formation was determined by image analysis and weighing of mature stroma. In the fruit experiment, both cultivars were inoculated with one isolate of M. fructigena and of M. polystroma. The pH changes were determined after 7, 14, 28 and 35 days of incubation in both healthy and inoculated fruits. The fastest mycelial growth was at pH 4.5 for M. polystroma and at pH 3.5 for M. fructigena. After a 30-day incubation, M. polystroma isolates produced twice or three times more stroma compared to M. fructigena isolates. For both brown rot fungi, the amount of mature stroma increased from pH 3.5 to 5.5, and then decreased at pH 6.5. Results of the.fruit experiment showed that healthy fruits were quite acidic (pH < 3.5), but pH rapidly increased in the inoculated fruits for both cultivars, reaching pH 4.6-5.4 depending on cultivar and fungus isolate. On both cultivars, the stroma developed at a significantly higher pH for M. polystroma than for M. fructigena. Biological and practical implications of the results are discussed.
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Comparative study of plum cultivars belonging to different taxons during 1980-1996
13-19.Views:158The program of the Research Institute of Cegléd, comprises the acclimation and selection of 7 Prunus-species and 39 cultivars grafted on Myrobalan C. 679 (P. cerasifera) seedling stocks. In addition two varieties have been investigated on three different rootstocks each: Myrobalan C. 174 (P. cerasifera), Bitter almond C. 449 (P. arnygdalus var. amara) and Sweet almond C. 471 (P. amygdalus var. dulcis). Fruits of commercial quality are produced maroly on some cultivars of P. salicina-, P. italica- and P. cerasifera character. Authors explain the three possible causes of low yields experienced in non-European plums:
1/ unfavorable environmental conditions
2/ lack of irrigation and
3/ superficial information concerning the variety, rootstock and adequate traditions as well as growing practices.
A rather tight correlation has been stated between blooming dates and the main ripening period. However, the early blooming time alone cannot be considered as the cause of low productivity. The decay of plum trees is attributed to special ecological requirements and phytosanitary problems of the foreign plum cultivars. The analysis of regression revealed stochastic relations involving several other characters too, which facilitate the planning of cross-combinations in the breeding program.
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First evaluation of fruit quality of some new and old sour cherry cultivars in Hungarian climatic condition
37-43.Views:305Indeed, in the last decade a large number of new sour cherry cultivars with new notable flavor characteristics have been released. There is no enough information related to qualitative characteristics which consistently segregated based on their predominant sensory characteristics. The present study carried out at tow consecutive years (2008 and 2009) and examined some qualitative characters which are the main importance in the specialty cherries trade, The main objective was to determine fruits quality of Érdi bőtermő, Oblacsinszka, Debreceni bőtermő, Csengődi, Pándy 279, Éva, Kántorjánosi, Újfehértói fürtös and Petri sour cherry cultivars. Changes observed in major characteristics such as fruit detachment force, width, length, weight, peduncle length, flesh firmness, brix, citric acid, Suger/acid ratio and pit weigh.While observed no change in flesh/pit ratio and Coordinate of colour (X*, y*, x*), hue angle and chroma value, in different years. In all measured factors, there was significant difference between selected cultivars of sour cherry fruit. Debreceni bőtermő showed higher values of fruit detachment force in 2008, while had the lover of fruit detachment force in 2009. Fruit firmness of “Érdi bőtermő” were monitored at lover value in both 2-year.
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Comparative anatomical study of leaf tissues of scab resistant and susceptible apple cultivars
43-45.Views:211According to previous studies some anatomical features seem to be connected with resistance or susceptibility to scab caused by Venturia ineaqulis (Cke./Wint.) in case of a given cultivar. Study of leaf anatomy of three scab resistant (‘Prima’, ‘Florina’, MR–12) and two susceptible (‘Watson Jonathan’, ‘Golden Delicious Reinders’) apple cultivars have been made. Preserved preparations made of leaves has been studied by light microscope. Studied parameters were: thickness of leaf blade, thickness of palisade and spongy parenchyma, thickness of epidermal cells, thickness of the cuticle. By measuring leaf thickness and epidermal cell thickness visible differences appeared in certain cultivars, while most conspicuous difference has been shown in thickness of the cuticle.
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Application of DNA markers for detection of scab resistant apple cultivars and selections
59-63.Views:113A DNA marker-based study was undertaken to identify the occurrence of major scab resistance genes in some apple cultivars and selections of importance for apple breeding. Unfortunately none of the RAPD-based markers previously reported to detect the Va, Vb, Vr and Vx genes produced unambiguous results. By contrast, the CAPS marker MI8 produced the expected three bands in all cultivars and selections already known or suspected to have the Vf gene, as well as in the Russian cultivar 'Antonovka Polotora Funtovaja' suspected to have Va resistance which however may be allelic to Vf. Vf-carrying selections and newly named cultivars 'Frida' and 'Fredrik' are grown successfully in Sweden without fungicides, suggesting that the Vr resistance breaking scab races 6 and 7 have not yet become a problem. The SCAR marker B12 detected the Vm gene in 'Prairifire', 'Rouville', clones 'OR45T132' and 'OR48T70', and selection '16-36-193'. The SSR locus 0102b10 detected one band at 118 by in 'Reka'. This is presumed to be identical to the Vr gene marker previously reported.
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Performance of sweet cherry cultivars grafted on Colt rootstock
7-10.Views:656In this paper growing characteristics and fruit bearing parameters of ‘Lapins’, ‘Kordia’, ‘Sweetheart’ and ‘Regina’ sweet cherry cultivars grafted on vigorous Colt rootstock were evaluated at the University of Debrecen, Pallag Experimental Station. Based on our data five years old trees can be described with homogenous strong growing, but very week yielding (2.1-3.1 kg/tree), as while fruit size varies between 26.2 and 27.2 mm. Producing the examined cultivars on Colt rootstock with high plant density requires higher attention and more interventions (root pruning, sawing the trunk) during the technology.
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Growing characteristics of apple cultivars in environmentally friendly growing systems
7-10.Views:255Nowadays the condition of the good saleability of the fruit is the application of controlled environmentally friendly technologies. Success of the growing is basically influenced by the production value of the cultivars, and their flexibility to the different technologies. In our examinations the effect of the integrated and organic farming system has been evaluated on the growing characteristics of the apple cultivars. According to our results the trunk thickness of the trees both in the initial and both in the later bearing period is higher in the integratedproduction compared to the organic farming system. This differences most likely caused by the variance of the crop load.
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Variation in selenium tolerance among two onion cultivars in closed fortification system
75-77.Views:218Selenium (Se) is an essential trace element for humans and animals. To consumption of selenium could be good sources the vegetables. Many of them are able to convert the inorganic selenium forms to organic forms, which are more effective for health. To enrich onion with selenium is known however some unclear points are remained. A closed fortification system was conducted to compare the difference of selenium tolerance in two onion cultivars in greenhouse environment. This system was well controlled, eliminating a lot of disturbing factors. The comparative analysis of Makói bronz and Makói lila spring onoins showed that there is difference in selenium tolerance and accumulation not only between plant species but inside it between the different cultivars, too. The Makói bronz seemed to more sensitive to the selenate treatment than Makói lila.
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Comparative evaluation of chamomile (Matricaria recutita L.) populations from different origin
91-95.Views:451In our research project under cultivation we examined 4 cultivars ('Soroksári 40', 'Lutea , 'Goral', 'Bona') and 28 wild populations of chamomile assuring all of them the same environmental conditions. Plant height, flower-diameter, essential-oil content, and the main terpenoid and flavonoid composition were analysed. The aim of our study was to establish the genetic background of breeding a new cultivar as well as encircle those natural habitats that give chamomile drug with the best quality.
In the case of plant height populations from the Great Hungarian Plain were lower than plants from Transdanubia and the control cultivars. Between the wild and the standard individuals we found significant differences with relevance to the flower-diameter. With regard to the essential oil content the populations were very heterogeneous even those, which came from the same habitat. Populations form Transdanubia and Nagyiván reached the essential oil level of the cultivars (0.721-0.931 gi100g), and 75% of the examined plants exceeded the minimum requirement of the PhHg VIII.
According to the essential oil composition our previous statement was confirmed that in the populations of Transdanubia and Northern part of Danube—Tisza Mid Region the main component is bisabolol-oxide A (30-41.2%), while plants native to the territory cast of the river Tisza are mainly characterised by a-bisabolol (32.3-48.4). In some samples the ratio of bisabolol-oxide B was more than 10%. The chamazulene content was higher in the cultivars selected to this component (above 20%), than in the wild populations (varied between 1.22 and 17.2%). Populations originated from the central part of Hortobágy region had extremely high apigenin content (10-13 mg/g), but in the case of chlorogenic acid, hyperoside and quercitrin we did not find any differences affected by the origin.
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Large variations in antioxidant capacity and contents of Hungarian sour and sweet cherry cultivars
25-28.Views:414Sour cherry (Prunus cerasus L.) cultivars grown in Hungary are of local origin while most sweet cherry cultivars in Hungary are introduced from other countries.A great phenotypic variability is displayed by both species. In the present study, we analyzed 10 sour and 9 sweet cherry cultivars for their antioxidant capacity, total polyphenolics (TPC) and total anthocyanin (TMAC) contents. In general, sour cherries showed higher levels of antioxidant capacity, TPC and TMAC. The anthocyanin contents varied from 0.16 to 6.85 and 1.41 to 127.56 mg/100 g for sweet and sour cherries, respectively. However, TMAC generally seems to have a limited influence on the antioxidant capacity of cherries.An amarelletype sour cherry, ‘Pipacs 1’ showed the highest antioxidant capacity (21.21mmolAA/l) and TPC (44.07mgGA/l) in contrast to its lowanthocyanin content. The detected diversity presents a choice that can satisfy different consumer preferences, and meet specific nutritional requirements.
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The effect of different carbohydrates on the multiplication of Hosta cultivars
115-117.Views:159The effect of seven concentrations of two carbohydrate sources were compared to determine the best source and the most suitable source and concentration for micropropagation of some Hosta cultivars: H. 'Gold Haze', H. 'Gold Drop' and H. 'Dew Drop'. 0, 5, 10, 20, 30, 40 and 50 g/1 sucrose or glucose were added to a MS basic medium supplemented with 3 mg/1 kinetin and 0.1 mg/1 IAA. For 'Gold Haze' 40 g/1 sucrose proved to be the best source and concentration, the proliferation ratio was 15 shoots per explant. Thirty g/1 sucrose concentration was the optimum for 'Gold Drop', the proliferation rate was 14.6 shoots per explant. In 'Dew Drop,' the best results were obtained with 30 g/1 sucrose but 40 g/l sucrose gave good results too. Both cultivars rooted well on these media. On glucose containing media, very low propagation rates were found in all concentrations and all examined cultivars.
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Nectar production of pear (Pyrus communis L.) cultivars
67-75.Views:301Detailed studies were made on the nectar production of 44, 16 and 18 pear cultivars, respectively, in a cultivar collection of pear during three consecutive years with highly different weather in the blooming. Results clearly show that pear does not necessarily produce small amount of nectar as stated in the world literature. In fact, pear can produce extremely high amount of nectar sometimes much higher than other temperate zone fruit trees species but its nectar production is highly subjected to weather, first of all to air temperature. Low nectar production seems to be more frequent than high one and cold weather can prevent its nectar production at all. On the other hand, results corroborate to the earlier statements on the low sugar concentration of pear nectar. There is a highly significant negative correlation between the amount of nectar produced by pear flowers and its sugar concentration (r = -0.52, n = 291, p< 0.001 for 1996, r = -0.34, n = 197, p< 0.001 for 1998). Sugar concentration in individual flowers may be up, to 40% in exceptional cases but generally it is well below 20%. Very high figures for sugar concentration in pear nectar at the literature seem to be incomprehensible. In contrast of some earlier statement in the literature no real difference could be established in the nectar production of pear cultivars, based on much more measurements than in earlier studies. Very low sugar concentration in pear nectar can explain the fact that the overwhelming majority of honeybees are pollen gatherers at pear trees even in the case of exceptionally high nectar production.
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DNA-based determination of suitable pollinating cultivars for the pear cultivar 'Carola' (Pyrus communis)
15-19.Views:141Pollen-limited fruit set has long been suspected in some relatively low-yielding orchards with the Swedish pear cultivar 'Carola'. Fruit was therefore harvested on 23 'Carola' trees in a commercial pear orchard. The seeds were germinated and five seedlings from each tree were sampled to determine which of the surrounding cultivars had been the most successful pollinators. Leaves of 'Carola', the 7 putative pollinating cultivars and the 115 seedlings were analysed with 6 RAPD primers. By comparison of the band patterns, paternity could be ascertained for 74 seedlings. The by far most successful pollinator was 'Clara Frijs' which had sired approx. half of the seedlings, followed by 'Herzogin Elsa', `Skanskt Sockerpiiron', 'Alexandre Lucas', 'Coloree de Juillet' and 'Doyenne du Cornice'. The latter is the maternal parent of 'Carola', and these two cultivars must therefore share one S-allele and hence can only be semi-compatible. In addition, 6% of the seedlings were in all likelihood derived from selling_ since they showed no bands that did not occur also in 'Carola'. Maximum distance between 'Carola' trees and suitable pollinators should not exceed 15-20 tn. Longer distances may produce a serious dearth of compatible pollen as evidenced by the large percentage of seedlings derived either from selling. (25%) or from long-distance (> 40 m) pollen transfer (25%) when 'Carola' trees were surrounded by non-preferred pollinators.
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Fruit Quality of Sweet Cherry Cultivars Grafted on Four Different Rootstocks
59-62.Views:194A rootstock trial planted at the Szigetcsep experimental station in 1989 involved the study of two cultivars- `Germersdorfi FL 45' and 'Van' -grafted on four different rootstocks — 'Colt', `MxM 14 — Brokforest', `MxM 97 — Brokgrow' and 'Saint Lucie 64' as a control. The trees were trained to the "Modified Brunner-spindle" system and came into bearing in 1993. The yield per tree, fruit weight and fruit diameter have been measured each year since then. The refractivity, the acid content of the fruit juice, fruit cracking after four hours' dipping in water and stone weight ratio have also been measured since 1995. In 1997 and 1998 these parameters as well as fruit cracking after 24 hours' dipping were measured. Fruit firmness and fruit colour were also estimated. In almost every observed parameter significant differences were found between the scion cultivars. Yield efficiency was significantly higher on 'NUM 14' and 'Saint Lucie 64' than on the other two rootstocks. As regards fruit weight, in both cultivars and over an average of six years, it was found that trees on low yielding tree on 'Colt' rootstock had the highest fruit weight values and on heavy producing `MxM 14' the smallest. Soluble solids content was higher on 'Colt' and `MxM 97'. No significant differences between the rootstocks were found in acid content of the fruit juice. There were significant differences between the rootstocks in fruit cracking after 4 and 24 hours' dipping in water. Seemingly with respect to cultivars and rootstocks the year has a considerable effect on fruit cracking.
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Genotyping Hungarian apricot cultivars for self-(in)compatibility by isoelectric focusing and PCR analysis
69-72.Views:173Self-incompatibility (SI) in flowering plants is a widespread genetic system that promotes out-crossing. In Prunus species the SI is a gametophytic trait, which is controlled by a single multiallelic locus, termed S-locus. S-alleles codify stylar glycoproteins with ribonuclease activity (S-RNases). Our objective was to assess the S-genotype of some Hungarian apricot varieties by isoelectric focusing of stylar RNases as well as by PCR analysis using cherry consensus primers. Consensus primers amplified one or two bands of various sizes. Primers amplifying the 1st intron gained fragments the size of which ranged from 250 to 500 bp; while those amplifying the 2nd intron resulted in fragments of 800-2000 by length. Our data demonstrated that the first intron of the apricot S-RNase gene is shorter than the second one, which coincides with the structure of cherry S-RNase alleles. `Hargrand' (S1S2) and `Harcoe (S1S4) possessed one common S-RNase isoenzyme. Hungarian 'Orias' apricot cultivars showed different bands compared to the previous cultivars, but they shared completely identical patterns confirming that they possess the same S-genotype. 'Bergeron', `Harmat' and 'Korai zamatos' are characterised by an evidently distinct S-RNase pattern. The self-compatible cultivar (`Bergeron') had one allele, which suggests its correspondence to the Sc. Primers for the 2nd intron was unsuccessful in gaining fragments, which indicates that the 2nd intron in the Sc allele is too long to get any amplification. On the basis of our data, identities and differences were revealed in the S-allele constitution of some economically important Hungarian apricot cultivars at protein and DNA levels.
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Incompatibility studies of Hungarian sweet cherry (Prunus avium L.) cultivars by traditional test crossings
43-47.Views:129Cross-incompatibility is a common phenomenon between various sweet cherry (Prunus avium L.) cultivars. Traditionally, choosing cross-compatible cultivar pairs is based on test crossings in the field.
There is a lack of information about fertility relations of novel Hungarian sweet cherry cultivars and selections. We have studied cross-incompatibility in 42 sweet cherry cultivar pairs by test-crossings in the field. Out of those, 3 combinations showed incompatibility and 15 pairs were compatible.
Test-crossing results proved that with the knowledge of S-allele constitution of Hungarian cultivars incompatible cultivar pairs are recognised in practice reliably. However, we assume that in sterility not only the S-gene system, but other factors (e.g. abnormal development of pollen or flower) also occur, therefore, their examination would be needed.