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• Tree growth and inner characteristics of fruits in pear cultivars

  29-32.

  J. Iváncsics

  Z. Vadas

  G. Jekkel

  Views:

  205

   

  Summary The aim of study was to investigate growth (crown growth and trunk diameter) and fruit characteristics [dry matter content (%), pectin (%), total acid (%) contents] of 10 pear cultivars ('Móri császár', 'Nyári Kálmán', 'Mogyoródi óriás', 'Fehérvári körte', 'Szegfa körte', 'Piroska', 'Mézes körte'). The crown development of the cv. 'Fehérvári körte' can be regarded as outstanding among the examined cultivars. The growth of the cv. 'Mogyoródi óriás' was low, this cultivar showed the poorest growth vigour. The width of the crown in several cultivars developed at a similar rate as the height of the crown. Outstanding values were shown by cvs. 'Fehervári körte' and 'Móri császár'. The development of trunk diameter was the highest for cvs. 'Piroska' and 'Hóka', and large growth of trunk diameter can be seen on cv. 'Fehervári körte'. Cvs. 'Mogyoródi óriás' and 'Mezes körte'showed weak growth. Outstanding dry matter content of fruits was measured on cvs. 'Fehervári körte' and 'Mézes körte'. The total acid content of fruits of cvs. 'Mézes körte' and 'Fehérvári körte'' was significantly different from the total acid content of cv. 'Mogyoródi óriás'. Pectin content was low in fruits of cvs. 'Mogyoródi óriás' and 'Fehérvári körte', while cv. 'Mézes körte' contained significantly more pectin. Vitamin C content we found was rather high in cvs. 'Mézes körte' and 'Fehérvári körte'.

   

• Assessment of soil characteristics in orchard

  11-14.

  A. Nagy

  T. Fórián

  J. Tamás

  Z. Szabó

  J. Nyéki

  M. Soltész

  Views:

  761

  The research field was at Siófok, in Hungary, which is situated in the South East side of Lake Balaton. The physical characteristic of the soil is sandy loam and loam and the peach orchard is irrigated. The detailed goals were mapping and analyzing of physical properties of the soil in water management point of view, mapping the acidity and CaCO3content of soil for precision liming, measurement of humus the element content. Sites with different physical characteristics (from sandy loam to loamy clay) could be distinguished. The reason for this is that besides the possible increase of clayminerals, the increasing rate of colloidal humus content contributes to larger soil plasticity. Statistics also proved positive and strong correlation (r=0.822) between the soil plasticity and humus content. In the case of pH, only a small part of the orchard is has to be limed, since most of the orchard has neutral pH, which is advantageous for nuts and stone fruits. It has to be mentioned, that the CaCO3 supply is also appropriate for the stone fruits. Based on the results hyperspectral imagery can be a good solution for detecting calciferous soils, although these measurements are still need validation.

• Cultivation systems in plum orchards

  125-129.

  I. Gonda

  Views:

  523

  In this review, we give an overview of recent cultivation systems in commercial plum orchards. The aspects of selecting the shape of the crown and the spacing of trees are discussed first and then growth characteristics and the structure of the crow are described in detail. Finally, the wound healing and regeneration characteritics of plum trees are detailed which have an influence on the applied pruning technique.

• Preliminary results of renewal pruning an 18 years old sour cherry trees

  41-42.

  B. Vaszily

  Views:

  275

  Sour cherry is a light demanding fruit species. As most of the crop is developing from buds on one year old shoots and 2-yearold wood, the formation of bare wood can be seen apparently in a badly maintained canopy. The formation of „whip shoots” indicates the degree of bare wood. Unfortunately in many gardens and commercial orchards sour cherry trees resemble willow trees. Owners often decide to grub old orchards, although the useful cropping lifespan of the trees planted in a correct spacing can be 20-30 years on a move vigorous rootstock. Therefore renewal pruning can contribute to the full renewal of the orchard, but the length of its effect is depending on the growth characteristics and renewal capabilities of the cultivar. The growth characteristics of the cultivars are different. The regeneration capabilities of different aged wood are also different. These differences emphasize the need for cultivar specifi c pruning. Knowing the reactions to renewal pruning, we can ensure regular high crops with excellent quality by applying cultivar specifi c rotation pruning.

• Changes of the characteristics of Satureja hortensis L. herb during flowering period

  56-60.

  M. Héjja

  É. Héthelyi

  A. Z. Ferenczy

  E. Szentgyörgyi

  Views:

  231

  In this study the changes of the characteristics of Satureja hortensis herb were investigated during flowering period, in case of savories of 4 different origins. The change of drying ratio, leaf/stem ratio, essential oil content, the ratio of several essential oil components (carvacrol, y-terpinene, p-cymol, a-pinene, p-pinene and fi-caryophyllene) were studied from bud formation till seed ripening.

• Translocation of diquat dibromide

  105-107.

  J. Mikulás

  J. Lázár

  G. Kazinczi

  Views:

  236

  The aims of our work were to answer the following questions: 1. Diquat dibromide at different concentrations is a contact or systemic herbicide? 2. if translocation occurs what is the extent and direction of it. 3. by what concentration it is translocated in hydroponics. It has been stated that diquat dibromide at different dilutions (40-5%) had systemic effect in Robinia pseudo-acacia in the fields. Its translocation has been occurred apically first, and later basipetally. The extent of translocation depended on the concentration. In hydroponics diquat dibromide has crytallized on the leaf surface of Galinsoga parvif lora and all concentrations (40-0.078%) proved lethal. Recognition and application of systemic characteristics of diquat dibromide is reported here for the first time. Improvement of application method is in the focus of our future work.

• Economics of sea buckthorn production and processing in Hungary

  21-25.

  A. D. Erdős

  L. Szőllősi

  Views:

  396

  This study focuses on the business management-related advantages and disadvantages of sea buckthorn production and processing based on economic analyses. It is the main objective of the authors to identify the expected economic findings in a high standard plantation with different average yields. A deterministic model calculation was performed on the basis of technological processes, using the primary data collected from enterprises dealing with sea buckthorn production. The calculation is based on the assumption of a 10 hectare plantation with intensive production technology (high soil quality (golden crown value: 32 GC per ha), irrigation, high plant density per hectare). The cost and income relations and the long-term return of the plantation were examined in the case of different average yields (12 t ha^-1, 18 t ha^-1 and 24 t ha^-1). Under the economic circumstances of 2016, the planting cost of an intensive plantation is around 4-4.1 million HUF ha^-1. In the years following the fruit-bearing stage, direct production costs are between 2.5-3.9 million HUF ha^-1, depending on the given average yield. On the contrary, 5.6-11.1 million HUF ha^-1 revenue can be reached based on the current market prices, resulting in a gross margin of 3.1-7.1 million HUF ha^-1. Under the modelled circumstances, return is realised on the plantation’s costs in 6-8 years. The net present value (NPV_r=3.24%) calculated for the 15-year-long life cycle of the 10-hectare plantation is between 151-466 million HUF, while the internal rate of return (IRR) is between 23-45%. From the business management aspect, the advantage of sea buckthorn production is that it provides better income and return at a planting cost which is similar to that of other small fruits and berries. At the same time, the disadvantage of sea buckthorn production is the fact that yields are harvested every two years due to the technological characteristics of harvesting. The negative impact of this bi-yearly yield on liquidity can be eliminated with the so-called delayed planting.

• Virus and virus-like diseases of grapevine in Hungary

  25-36.

  J. Lázár

  Gy. D. Bisztray

  Views:

  409

  Viruses and viroids are submicroscopic infectious particles which can cause disease symptoms on grapevine. These parasites are depending completely on the energy metabolism of the plant cell. To enter the host cell plant viruses depend on injuries or on transmission via invertebrates (insects, nematodes, etc.). Viruses are classified by many characters including particle morphology, host range and information content of the genome. At present about 70 viruses including 7 viroids infecting grapevine are known. In single or mixed infections they are potentially detrimental to the quality and quantity of grape production in any growing area of the world. Some viruses can cause severe economic damage in vineyards. In Hungary many important viruses and viroids have been detected in grape. This review summarises characteristics of viruses and the results of detection and characterization of virus and virus like diseases of grapevine in Hungary. The identification of the causal agent, its transmission, geographical distribution and the development of the diagnostic methods are also discussed.

• Modelling and comparing two canopy shapes using FEM

  71-74.

  Z. Láng

  L. Csorba

  Views:

  168

  Central leader and Vase form canopy models were built using FEM. Their main characteristics were chosen to be the same. The models were virtually exposed to the effect of steady-state horizontal forced vibration in the frequency range of 0-20 Hz. Acceleration-frequency curves were calculated and drawn to find the best frequency values for the effective detachment and also to see the acceleration differences in the limbs. For the same purpose the direction of shaking was also changed. It was found that for the Central leader canopy shape multidirectional shaking would bring uniform detachment while for the Vase form trees also the unidirectional shakers were appropriate. The acceleration achieved for the Vase form models were much higher than for the Central leader type. The acceleration-frequency curve of the shaker unit can be used to find the best frequency for shaking.

• Improved clonal approaches to growing black locust (Robinia pseudoacacia L.) in Hungary: a case study

  53-56.

  K. Rédei

  Zs. Keserű

  B. Antal

  Views:

  429

  In Hungary black locust (Robinia pseudoacacia L.) is considered as an important exotic stand-forming tree species and due to climate change effects its importance is increasing in many other countries. It has some desirable characteristics from both the practical and research standpoints. As a result of a partly new black locust selection programme new black locust clones were improved and a technology was developed for mass clonal micropropagation of juvenile trees. Clone trials with micropropagated plants were established in the country for evaluating the juvenile growth and the stem form of promising black locust clones under marginal site conditions. Significant differences (P<5%) were found for stem form value which partly verified the genetic gain of the selected clones against the common black locust. It was also proved that tissue culture could offer partly new prospects for the rapid mass cloning of selected genotypes.

• The importance of Hungarian melon (Cucumis melo L.) landraces, local types and old varieties (Review)

  83-87.

  Cs. Szamosi

  Views:

  232

  While supermarkets devote whole aisles to hybrids, traditional varieties are hard to find, and becoming scarcer day by day. Unfortunately, countless old melon varieties have already been lost. Luckily we succeded in collecting most of these varieties, and thus conserving them in Gene Banks. Landraces, local types, and old breed races show many characteristics that could be useful in organic farming. It is important to get acquainted with these varieties and cultivars, because they have greatly adapted to the climatic and pedological conditions of the Carpathian basin. Therefore their conservation is essential for the protection of Hungarian genetic variability. With the help of utilising our landraces in organic farming; careful selection; and the usage of marketing strategy in order to enhance quality features, such as unique flavour; we could reclaim the one-time excellent reputation of Hungarian melon.

• Healthcare values and potential uses of the new Hungarian apple varieties on the basis on fruit analysis

  25-28.

  G. Ficzek

  M. Ladányi

  Zs. Radeczky

  M. Tóth

  Views:

  363

  Biological active compounds and valuable characteristics of some apple varieties and candidates were measured in our trials. Fruits of ’Rosmerta’, ’Hesztia’, ’Cordelia’ and ’Artemisz’ are recommended to enrich the Hungarian assortments for fresh consumption and choice of new tastes. Based on examined parameters it can be assumed that novel Hungarian resistant varieties are suitable for juice and fruit concentrate production, and due to high pectin content of their remaining pomace these varieties can be raw material of pectin production as well as they are also suitable for jam production mixed with other fruit species. Furthermore, functional food industrial product having high quality can be produced by using novel resistant varieties because of their high pectin and polyphenol content. Beside of their high market value their suitability for growing among orchard conditions is confi rmed by lower costs of production because of less plant protection treatments.

• Test of the utility of apple retrotransposon insertion patterns for molecular identification of 'Jonathan' somatic mutants

  7-10.

  Zs. Galli

  B. Wichmann

  E. Kiss

  T. Szabó

  L. Heszky

  Views:

  472

  Up until today, apple sport mutants proved to be indistinguishable from each other and their progenitors at the molecular level using random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) marker techniques. This is not surprising, since the genomes of these somatic mutants differ only in one or a few small regions that affect economically important characteristics, such as improved fruit colour, size, or flavour. In most cases, these genome differences are probably caused by retrotransposons which are able to convert their RNA transcripts to DNA with reverse transcriptase enzyme prior to reinsertion, but unable to leave the genome and infect other cells. Retrotransposon insertions can alter the expression of other genes and/or the structure of encoded proteins. The sequence-specific amplified polymorphism (S-SAP) technique is capable of revealing the genetic distribution of retrotransposable elements over the whole genome. The present study used this approach to try to characterize and distinguish 'Jonathan' somatic mutants via fingerprinting, which is an unsolved problem.

• First selections of the Hungarian apple breeding program for multiple resistance

  9-13.

  M. G. Tóth

  Szabó Kovács

  K. Kása

  Zs. Rozsnyay

  M. Hevesi

  Views:

  337

  The aim of the first Hungarian apple breeding program for multiple resistance started in the beginning of the nineties is to widen Hungarian apple assortment by good quality, resistant apple cultivars with excellent productivity and ecological capability to the most important fruit growing areas in Hungary. In the first years of seedling production we made early selection for susceptibility to apple scab in greenhouse. Alter this, field observation of susceptibility to powdery mildew, scab and canker and a yearly negative selection was carried on. From 1997, fruit quality was evaluated as well, and from 2001 the resistance of shoots to Erwinia ainylovora (Burrill/Winslow et al.) was examined using inoculations in greenhouse conditions. From the progenies of crosses in 1992 and 1993, six candidates were announced to national recognition out of hybrids examined for more than a decade. Descriptions of these selections from 'Prima' progenies and the most important data of their resistance, growing habit, morphological characteristics and fruit quality are shown in this article.

• Comparative study of the vegetative and generative organs in pear varieties

  21-25.

  P. Dremák

  G. Kocsisné Molnár

  Views:

  244

  An assortment of 17 pear varieties was examined in 2006 at Keszthely, Department of Horticulture, Georgicon Faculty of Agriculture, Veszprem University. The selected varieties were planted in 1980, grafted on seedling rootstock and represented the majority of existing pear plantations in Hungary. The main objective was the determination of suitability of the most important varieties for the purpose of intensive growing technologies even when grafted on vigorous seedling rootstock. The most important growing and fruiting characteristics of the varieties have been assessed and evaluated from the point of view of productivity. We stated that the relations of the trunk or the main axis to the lateral branches and fruiting structures are all subject to varietal effects and are valuable indices of the growing character. The quotient of the diameters of trunk and branch should be around 0.3-0.4, and the relative frequency of fruiting structures (Dárda, nyárs, vessző) meaning the ability of branching and regeneration associated with accurate pruning policies are decisive from the point of view of promising success.

• Evaluation of essential oils by gas-chromatography and a new method: "electronic nose"

  85-89.

  I. Novák

  N. É. Zámboriné

  H. Horváth

  Zs. Seregély

  K. Kaffka

  Views:

  252

  The chemical quality of the essential oils is determined by their composition as well as by the complex aroma features. For the evaluation of odour and aroma, sensory tests are practised, especially for applications such as aromatherapy, food industry or perfumery. In the recent studies we investigated eight Mentha origins (populations of Mentha iperita, M. spicata and M. arvensis) of our genebank collection using a new and effective instrumental sensory evaluation method parallelly with the usual GC analysis.

  The results show that the examined mint clones possess different essential oil accumulation levels and special flavour characteristics, too. The GC analysis of the essential oil revealed characteristic differences at both inter- and intraspecific level in the proportion of mentol, menthon, carvon, limonene, menthyl acetate and in two not identified monoterpenes.

  In the sensory tests the special complex aroma enables a reliable distinction with a single exception among the populations by the help of the "electronic nose" equipment. The distinction among the samples based on the sensor signals of the instrument — evaluated by multivariate methods — shows a close relation with the detected monoterpene components of the essential oil.

   

• Work quality assessment of a cherry sorting machine

  57-62.

  E. Ancza

  Z. Csizmazia

  N. I. Polyák

  E. Farkas

  J. Nyéki

  Z. Szabó

  Views:

  823

  The conditions of the cultivation of marketable cherries are diverse. Choosing the production site on the basis of the climatic conditions, selecting the most appropriate variety taking into account the region and the purpose of the production, utilization of the optimal production method, to ensure the required water and nutrients supply, frost and hail protection techniques, modern technology in crop protection, and professional performance of harvesting and handling, to name just a few of the most important issues. The objective of present study is to determine the qualitative characteristics of the UNITEC cherry sorting machine.

• Nutritional values of traditional open-pollinated melon (Cucumis melo L.) and watermelon (Citrullus lanatus [Thumb]) varieties

  29-31.

  Cs. Szamosi

  H. Némethy-Uzoni

  G. Balázs

  É. Stefanovits-Bányai

  Views:

  440

  The aim of our experiments was to investigate the internal quality parameters of some old melon and watermelon landraces or varieties whether they have any desirable characteristics. Measurements were carried out using 8 different types of melon (Cucumis melo L.) and 8 accessions of watermelon (Citrullus lanatus [Thumb]), which were self pollinated under greenhouse conditions. Besides the determination of dry matter content; soluble solids, titratable acidity of the fruit juices, investigation of carbohydrates, total phenol content was measured by spectrophotometer. Total antioxidant capacities were determined spectrophotometrically using the FRAP method. We found some accesions both among melon and watermelon varieties, which could be characterized by outstanding inner content.

• Chemical characteristics of nectarine genitors used for breeding program in Romania

  41-43.

  A. Ivascu

  Views:

  186

  The geographic location of Bucharest area, the nature of its soil and its climate offer remarkably favourable conditions for nectarine growing, with juicy and well flavoured fruit, good for fresh consumption and processing.

  The five years' investigation (1996-2000) has helped to identify some nectarine cultivars and hybrids (NJN 58, ARK 165, ARK 139, Morton, ARK 107, Romamer I, ARK 125, Firebrite) with exceptional fruit quality (dry matter content, titratable acidity, vitamin C content, overall sugar, and amount of pectic substances).

  Dry matter content varied over the three years from 8.3 to 18.5% (NJN 68), meanwhile titratable acidity varied between 0.26 and 1.08% (Romamer I). Sugar / acidity ratio was within the limits of 8.6 and 36.5 (Nectared 7). Nectarines have high vitamin C content, over 10 mg/100g (Fairlane, NJN 67, Regina, Harko), glucides were found in Firebrite, Romamer I varieties and NJN 21, CIR1T127, HFSR3P4, HNA hybrids.

  Some cultivars have been recommended for planting in this southern area of Romania (Crimsongold, ARK 125, Harko, Hardyred, ARK 134), others used as quality genitors (ARK 85, Nectared 7, NJN 21, ARK 21, ARK 134, Fantasia) in breeding projects.

• The after-effect of paclobutrazol on morphological characteristics of in vitro Narcissus poeticus ssp. radiiflorus plants

  43-46.

  M. Jevcsák

  M. Ördögh

  E. Jámbor-Benczúr

  Views:

  343

  After different pre-culturing period (12, 23 or 34 days) on ½ MS medium with 1 mg l-1 paclobutrazol, 1 mg l-1 N6-benzyladenine and 0.1 mg l-1 1- naphthaleneacetic acid , 3 groups of Narcissus poeticus ssp. radiiflorus bulb scales were kept on the same medium without hormones. The results were evaluated monthly and the final one happened after 7 month. The best results were achieved due to the shortest pre-culturing period (12 days;  Group 1), with 4.9 bulblets and 4.54% hyperhydricity. The result of the second treatment (pre-culturing period of 23 days; Group 2) was not different significantly but the number of bigger bulblet were higher (4.54 bulblets). After the longest pre-culturing period (34 days; Group 3), the number of bulblets was low (3.68) and more hyperhydricity (18.18%) was detected. The highest number of roots (13.91) was observed in this group
  very likely due to the strong after-effect of paclobutrazol.

• Relations of phenometrical indices of apple fruits with weather variation in the assortment of varieties of an apple gene bank

  115-120.

  L. Lakatos

  T. Szabó

  Z. Szabó

  M. Soltész

  Z. Sun

  J. Nyéki

  Views:

  625

  The purpose of phenometry is to examine the measurable parameters of the plants in order to follow up the consequences of weather processes. We should fi nd the reasons, why the diameter of fruits grows larger in one season and smaller in the next. Variation may occur as a response to insuffi cient provision of water or nutrients, but also because of pathological effects and of extremely high or low temperatures, moreover, of extraordinary heavy fruit load. There are phenometrical characteristics, which consider the fi nal consequences (density of fl owers, fruits set, drop of fruits), whereas other parameters could be followed up (size, length and width of fruits) as the dynamic components of growth. The quantitative parameters of growth are functionally related to each other, where the weather conditions, soil humidity and nutrients are on the input side, thus it is possible to model the growth of fruits as a function of the environment. Initially, the relations between the main weather variables and the phenometrical data have to be cleared. In the present study, the interactions between the mentioned phenomena are presented and numerically defi ned.

• Microclimatic studies on different aged apple plantations

  7-11.

  L. Lakatos

  I. Gonda

  Z. Szabó

  S. Zhongfu

  Y. Wang

  J. Nyéki

  Views:

  750

  The purpose of measuring parallel canopy and out of canopy microclimates was to find out in what extent climatic parameters measured in different aged canopis differ from each other and from the values characteristic to out-of-canopi areas. The importance of phytoclimatic researches seems to lie in the fact that if the reactions of fruit trees towards meteorological elements are continuously followed, we have the possibility to provide growers with information. These pieces of information are like defining the optimum time of phitotechnical interventions (summer pruning, sorting sprouts, thinning fruits, etc.), the necessity of applying mulching, defining the method and time of irrigation and applying plant protection activities. By means of phytoclimatic researches, it is possible to react to unfavourable meteorological impacts within a certain extent. It is also possible to successfully reduce the risks of late spring and early autumn frost damage, as well as the risks, content and measure of experienced heat and water stress conditions by finding out about the physical characteristics of the canopis' internal area.

• Floral biological studies on pear cultivars in relation to fire blight susceptibility

  25-30.

  A. Farkas

  Zs. Orosz-Kovács

  T. Bubán

  Views:

  357

  Floral biological characteristics that may influence cultivar susceptibility to fire blight were studied in 10 pear cultivars in two Hungarian orchards from 1999 to 2003. The receptaculo-ovarial, automorphic nectary is usually bigger in tolerant cultivars than in susceptible ones. Nectary stomata are meso- or xeromorphic. Susceptible cultivars tend to have more xeromorphic stomata, where guard cells are located 1-3 cell rows below the epidermis. The size of nectar chambers is usually smaller in susceptible cultivars. Floral nectar, consisting mainly of glucose and fructose, is more abundant and less concentrated if the cultivar is susceptible to fire blight. The amount of chlorogenic acid was higher in the flowers of tolerant cultivars than in susceptible ones.

• Estimation of plum and prune cultivars with morphogenetical traits

  147-152.

  D. Surányi

  Views:

  341

  The author post few years organizes the plum breeding program. He uses up earlier elaborated morphogenetic and productive characteristics and traits, already on such basis their selected 21 cultivars in the pomological garden at Cegléd. The Hungarian plum assortment and offer very specific and tight, thus that they endeavor, that the selection before then flare. There are indicating already only the perspective cultivars in present paper on basis of gathered data one decade underneath. Paralleling control was three type of used (Besztercei szilva Bt. 2, Green gage and Stanley). 12-sort trait according to were compared the cultivars. The productivity-biological standpoints without, the open pollination and pollen germination, over and above that the ripening on date big differences appear. The near future the 'C. 1501' (Yellow Besztercei), 'Ontario' and `Mirabelle de Nancy' (yellow plums), the yes untimely `Precoce di Guigno' (red plum), the very well abundant 'C. 940' and Victoria (lilac plums), over and above that 'Beregi datolya', ‘Révfülöpi’ and `Szarvasi' (blue plums) cultivars setup suggest. The demonstrated cultivars out of further give for deputize value the Precoce di Giugno, as earliest ripening and the 'Beregi datolya' but the at the latest ripening.

• Effects of aromatic cytokinins on structural characteristics of leaves and their post- effects on subsequent shoot regeneration from in vitro apple leaves of 'Royal Gala'

  41-46.

  J. Dobránszky

  E. Jámbor-Benczúr

  M. L. Reményi

  K. Magyar-Tábori

  I. Hudák

  E. Kiss

  Z. Galli

  Views:

  345

  The effects of different aromatic cytokinins applied in different concentrations and combinations were investigated on the histology of in vitro apple leaves and their post-effects on subsequent shoot regeneration from these leaves were studied. Great differences in the anatomical structure of leaves could be detected originating from media containing different types and concentrations of aromatic cytokinins. The number of regenerated shoots per explant and the organogenetic index were used for the evaluation of the post-effect of aromatic cytokinins on shoot regeneration. The histological structure of leaves used for regeneration and their regeneration response showed a good correlation. When the pre-treatment caused a juvenile-like or less-differentiated structure, the number of regenerated shoots per explant increased and often vitrification also decreased and consequently the organogenetic index also increased. A strong interaction between cytokinin-content (type and concentration) of the pre-treatment medium and that of the regeneration medium could also be detected.