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Volatile constituents of Nepeta cataria L., N. glechoma Benth. and N. parviflora M. Bieb. from Hungary
47-50.Views:128In the temperate zone live about 150 species of the Nepeta genus. Our investigations covered the examinations of the volatile oil containing species of the genus endemic in Hungary, Nepeta cataria and Nepeta parviflora. Latter is a relict of the ancient steppe-flora and endemic in Hungary as well. Phytochemical examination of the volatile oil containing plant material has also been carried out. Catnip growing in the Botanical Garden of PTE Department of Botany contained 0,67% volatile oil in May and 0,14% in November. Chemical character of the volatile oils were measured by gas chromatography/mass spectrometry and citronellol, citral-A, citral-B and geraniol components were identified. The composition of the oil of November samples shifted towards citronellol (65%). In both samples insecticide and repellent activity bearing compounds (+)-cis-p-menthane-3,8-diol, and (—)-trans-p-menthane-3,8-diol in 2-2.5 and 4-4.5% amount have been found. The catnip sample deriving from Germany contained a small amount of anetol, citronellol, neral, geraniol and geranial (6-13%), and possibly two isomers of nepetalactone in 23-31%.
The Nepeta parviflora endemic in the Nagyvolgy valley near Nagykaracsony consisted of the same compounds in the investigated years (1998-2000). Its limonene, methyl chavicol, b-cariophyllene, b-selinene, b-cubebene, davanone, germacrene-D constituents have been identified. In the year 2000 different GC % of these compounds were detected in the different organs of the plants.
The closely related species Nepeta cataria var. citriodora contained 83% citral, and the N. glechoma (= Glechoma hederacea) contained 41% a-cubebene, 20% patchoulenol, 7,7% spathulenol respectively. These compounds were identified by gas chromatography and gas chromatography / mass spectrometry.
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Applicability of 3D laser scanning in precision horticulture
55-58.Views:479Due to the technological development, remote sensing instruments and methods have become widespread in all segments of life (from precision agriculture through architecture to medicine). Among the innovative development of remote sensing instruments the 3D laser scanner is of outstanding importance. Horticultural applicability of terrestrial laser scanning technique is a new innovation in the precision agriculture. The structure of trees and branches, the canopy extension, the fruit yield, which can help to recognize some biophysical parameters, can be determined. The examination was carried out with Leica ScanStation C10 terrestrial laser scanner in the Study and Regional Research Farm of the University of Debrecen near Pallag. In this article the measuring principle, the parameters and horticulture applicability of the terrestrial laser scanner are presented.
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Epiphytic microbiota of apple in integrated and organic growing
85-89.Views:224The surface microbial contamination is of great interest in case of fruits, since they are they potential sources of spoilage or foodborne diseases. The aim of this work was to compare the epiphytic microbiota of food safety concern of different apple cultivars as a function of cultivation methods (organic versus integrated), production year and place. Investigating 47 samples it was found that the average microbial contamination of the apple surfaces was within a certain range,, practically independent of cultivar, growing area and year. The frequency distribution of the surface bacterial, mould and yeast counts did not show significant differences between growing technologies or varieties. The principal component analysis the samples ranked the apple samples into seven groups on the basis of their microbial contamination level, and the discriminant analysis proved the goodness of grouping. The grouping was independent from cultivars and growing methods. No foodborne pathogen Salmonella spp. and Listeria spp. were found on the surfaces of apples.
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Terminology of fruit set and fruit drop of sour cherry cultivars
33-36.Views:240Fruit set and fruit drop rates of 9 sour cherry cultivars (‘Érdi bôtermő’, ‘Debreceni bőtermő’, ‘Kántorjánosi’, ‘Újfehértói fürtös’, ‘Éva’, ‘Petri’ ‘Oblocsinszka’, ‘Pandy 279’ and ‘Csengôdi’) of eight years-old trees grown in Újfehértó, located in the Eastern north part of Hungary which grafted on Prunns mahaleb have been studied. Significant differences have been found in fruit set among cultivars. The average percentage of fruit set was 18.3%, which the ‘Oblacsinszka’ by 32.6% the highest fruit set, while ‘Debreceni bőtermő’ is very similar to ‘Pandy279’ showed lowest (12%) fruit set. Seasonal changes of fruit set and drop shows that there are four abscission peaks. The first fruit abortion wave appears during second week after pollination Thereafter, the second and third dropping period was found during the third and forth weeks after pollination. The forth abscission happened on the forth week after pollination. The highest fruit drop happened on ‘Pandy279’ (92.4%) very similar to ‘Éva’ (90%) while the lowest fruit drop observed in ‘Oblocsinszka’ (71.5%)
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Comparative evaluation of chamomile (Matricaria recutita L.) populations from different origin
91-95.Views:450In our research project under cultivation we examined 4 cultivars ('Soroksári 40', 'Lutea , 'Goral', 'Bona') and 28 wild populations of chamomile assuring all of them the same environmental conditions. Plant height, flower-diameter, essential-oil content, and the main terpenoid and flavonoid composition were analysed. The aim of our study was to establish the genetic background of breeding a new cultivar as well as encircle those natural habitats that give chamomile drug with the best quality.
In the case of plant height populations from the Great Hungarian Plain were lower than plants from Transdanubia and the control cultivars. Between the wild and the standard individuals we found significant differences with relevance to the flower-diameter. With regard to the essential oil content the populations were very heterogeneous even those, which came from the same habitat. Populations form Transdanubia and Nagyiván reached the essential oil level of the cultivars (0.721-0.931 gi100g), and 75% of the examined plants exceeded the minimum requirement of the PhHg VIII.
According to the essential oil composition our previous statement was confirmed that in the populations of Transdanubia and Northern part of Danube—Tisza Mid Region the main component is bisabolol-oxide A (30-41.2%), while plants native to the territory cast of the river Tisza are mainly characterised by a-bisabolol (32.3-48.4). In some samples the ratio of bisabolol-oxide B was more than 10%. The chamazulene content was higher in the cultivars selected to this component (above 20%), than in the wild populations (varied between 1.22 and 17.2%). Populations originated from the central part of Hortobágy region had extremely high apigenin content (10-13 mg/g), but in the case of chlorogenic acid, hyperoside and quercitrin we did not find any differences affected by the origin.
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Some physical properties of apricots and testing apricot sorting machines
51-62.Views:335Examinations were carried out in the manipulating and packaging plant of Gyümölcsért Ltd, in Boldogkôváralja, to determine some physical properties of five apricot cultivars and to test the work quality of the apricot sorting machines. The size and the weight of the fruits were measured and two sorting machines were tested. The results are given in tables and diagrams. The conclusions are also summarised.
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Cooling irrigation as a powerful method for microclimate modification in apple plantation
33-37.Views:286Irrigation in some countries is a horticultural practice mainly used only to supply water. At the same time the use of microsprinklers have a powerful influence on the changes of temperature in orchards. When the air’s temperature is high (about 20 °C or higher) the evaporative cooling irrigation significantly decreases the plants’ surface temperature and air temperature. The cooling effect is stronger when the air is dryer. By using cooling irrigation regularly, canopy temperature can be decreased so that the beginning of blooming can be delayed. Also if the blooming is early and frost probability is high, serious damages can happen in orchards. The beneficial effect of cooling irrigation is the temperature reduction and frost protection. InMarch 2010, one month earlier than the expected blooming an irrigation system was established to produce anti-frost treatment and regulate the micro-climate of a Gala apple orchard which belongs to the University of Debrecen (Hungary). The objective of sprinklers was to cool the air by increasing water evaporation and relative humidity. The position of the micro-sprinklers was planned in three levels (around the tree trunks, a few cm near to the soil surface, in the crown region and above the crown, a half meter higher). The results showed that the water sprayed in the orchard by micro-jets influenced decisively the temperature of the plantation. At higher temperatures (around 20 °C), the drop of temperature may attain 5–7 °C. A low relative humidity of the air may increase the relative effect. When water was applied at intervals of 15 minutes for ten times a day from 8 am to 18 pm, the air, flowers and bud’s surface temperature could be kept low.At certain days when the temperature was higher than 10 °C, irrigation was used at night time in similar 15 minutes intervals, from 18 pm and 6 am. The beginning of bloom could be delayed for more than ten days. The Gala apple variety blooming dynamics was characterized by a logistic curve in the treated as well as in the control plot. In the treated plot, the curve was steeper than in the control one in spite of the equal temperatures measured in the plots. Under Hungarian climatic conditions, the method was successfully used to delay blooming dates. The main result was the diminution of the frost damage in the spring that assured apple yields.
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Ecological drought resistance and adaptability of apple varieties
113-122.Views:428For adequate yields in apple plantations, during the long growing period of the fruit primordia, one of the decisive factors is water supply. Indicators of stress are valuable signs for the diagnosis of drought and necessity of watering, i.e. planning the irrigation of plantations. The aim of the present study was to find reliable signs of water stress on apple trees and at the same time conclude on the drought tolerance of different varieties. The plantation of apple varieties grafted on various stocks and cultivated according to different systems (irrigated, non irrigated, integrate and biological) has been examined continuously by leaf analysis. Along the period of growing fruits, measurements were made in the field, then the leaf samples were analysed in the laboratory for composition of pigments, carbohydrates and antioxidants, as well as the histology of the tissues checked. Without irrigation, the mean leaf mass and the relative chlorophyll content (SPAD) of the variety Idared on M4 stocks increases beyond the values of 50 SPAD, whereas on M26 stock and integrated system, it declines. In the collection of varieties, grown according to the „integrated” system, during the development of fruits, ‘Gála’ and ‘Remo’ varieties have been affiliated to the “less susceptible” group regarding drought tolerance because of the leaf morphology, structure and content of SPAD and antioxidants. However, ‘Idared’ and ‘Jonagold’ belong to the “susceptible” group. To the same group are ranged the ‘Akane, Red Rome vanWell, Pink Lady’ varieties. Those varieties reacted to a short period of drought by increased production (content) of carbohydrates. Regarding changes of carbohydrate content ‘Greensleeves’ and ‘Ozark Gold’ varieties belong to the moderately water dependent (requiring) group. In the field, SPAD-tests facilitated the measurement of nutrient-uptake and incorporation, which is proved by the tight correlation between the data of SPAD and the increment of leaf weight (r=0.76–r=0.88), however, this depends on the variety too. SPAD is an indicator of water supply and is related with the density of stomata, cannot used for the selection of water-exigent varieties but for (drought) tolerant ones. In integrated culture, the (drought) susceptible varieties display (water-soluble) hydrophilic and lipophilic antioxidants (ACWand ACL resp.) the quantity of which may have some role in drought tolerance.
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New sour cherry cultivars selected from local sources
79-80.Views:296New sour cherry cultivars selected from local sources
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Effect of different flower thinning techniques on annual fluctuation of macro and micronutrients in sweet cherries
87-90.Views:274The seasonal dynamic of macro- and micronutrients uptake of a sweet cherry cultivar cv. 'Katalin' (Prunus avium L.) was studied according to apply different flower thinning techniques. Beside control treatment, three thinning treatments were performed: (I) thinning for I flower/inflorescence, (2) thinning for 2 flower/inflorescence, (3) thinning for 3 flower/inflorescence. Soil examination was carried out to establish the growing conditions of orchard site. Moreover, for studying the temporal dynamic of nutrient uptake plant analytical examination was performed four times per year based on leaf collecting according to the phenological phases. It was found that the macro- and micronutrients contents of leaves were showed significant differences between treatments before ripening, at ripening and after ripening stage. It was found that thinning has influence on mineral composition of leaf. Flower removal unbalanced the equilibrium of generative and vegetative processes. The applied manual flower thinning treatment resulted improving vegetative processes like nutrient uptake and storage.
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Hungaricum as a quality of fruits and fruit products
71-81.Views:224The territory of the Hungarian state is largely suitable for the purpose of growing fruits of the temperate zone species. During the next decennia, the annual volume of Hungarian fruit production is expected to be around 1.1-1.3 million tons, from which some 15% is considered to be a produce of Hungary or "Hungaricum" (90 thousand tons of sour cherry, 50 thousand tons of apricot, 20 thousand tons of raspberry, 10 thousand tons of walnut). These fruits symbolise the country's special quality, which are worth to catch the interest the foreign consumers.
The category of Hungaricum involves almost exclusively varieties of Hungarian origin as sour cherries, apricots, raspberries and walnuts, and they are representing outstanding qualities on the international markets.
As for the fruit products the fruit brandies are eligible to be "Hungaricum" and are called exclusively "Pálinka". The Pálinka, provided to be distinguished with a geographic mark and will be competitive on the world market. Smaller quantities, though significant produce is represented by the deep frozen raspberry.
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Preliminary results on salicylic acid treatment on brown rot caused by Monilinia laxa on Jumbo Cot fruit, Prunus armeniaca L.
37-39.Views:381The effect of salicylic acid on reducing brown rot caused by Monilinia laxa infection in postharvest apricot fruit Prunus armeniaca L. Freshly harvest Jumpo Cot fruit were treated with water as control treatment and other group treated with salicylic acid 2 Mm as induced resistance treatment, Fruits were infected by M. laxa (1 × 10-3spores ml-1) and incubated at 25 oC for 2, 4 and 6 days. Treatment fruits with salicylic acid resulted in direct effect on mycelial growth as in the salicylic acid treatment the growth reached to 45% after 6 days of incubation while in the control treatment it reached to 100%.
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Comparison of pear production areas from yield risk aspect
25-28.Views:390There are three main pear production regions in Hungary. The most relevant is theWest-Transdanubian (Zala, Vas and Gyôr-Moson-Sopron counties), where up to 30% of total pear production occurs. The second most productive region is Pest County, where pear is grown mostly in gardens and garden plots, resulting in 15-20% of Hungarian production. In the northern Hungarian region (Bodrog valley in Borsod-Abaúj-Zemplén, Heves and Nógrád counties), the microclimate is perfect for optimal pear production. In our analysis, we focused on four plantations that are dominant in pear production in Hungary. Two of them are situated in south-western Hungary, one of them is in South Transdanubia and one is in North Hungary. Considering the personal attitude of the decision maker towards risk, the best alternative is ‘Williams’ in Alsóberecki, as the yield risk is the lowest with this variety, while the second best alternative is ‘Bosc Beurre,’ also produced in Alsóberecki. This is an irrigated area, and this fact evidently decreases the yield risk. The highest risk is in Bánfapuszta and in Zalasárszeg, for the non-irrigated ‘Williams’ variety. The highest yield with the lowest risk can be obtained with irrigation. Nevertheless, in the case that relevant data are available, and by incorporating cost and expected profit data, the stochastic dominance method is suitable for financial risk assessment, as well.
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Fruit bearing shoot characteristics of apricot and sweet cherry cultivars in Hungary
107-110.Views:298: Our study was carried out on 23 apricot and 9 sweet cherry cultivars in February 2005. Fruiting laterals were classified into four groups (0-10 cm, 10-20 cm, 20-40 cm and >40 cm) and then the density and setting of flower buds were evaluated and expressed as bud/cm. The flower bud density of four types of fruit bearing shoots and the changes in the frost resistance were studied. Shoots were collected from a young orchard in Gone (apricot), Siófok (sweet cherry) and Nagykutas (sweet cherry). There were significant differences among the cultivars in the density of flower buds. The number of flower buds/cm shoot length ranged between 0.91 and 2.20 in the average of the different fruit bearing shoot types on apricot. Based on the results, the bud density of shorter shoots is generally higher on apricot, but this is not valid for all cultivars. For cvs. Magyarkajszi and Ceglédi bíborkajszi, the highest flower bud density was detected on shoots of medium length (10-40 cm). There were fivefold and almost twofold (1.85) differences in bud density among cultivars on shoots shorter than 10 cm length and longer than 40 cm length, respectively. The ratio of the bud densities of the different types of shoots also ranged between wide boundaries. For cvs. Bayoto, Toyesi and Toyiba this ratio was 2.5-3.5, while for cv. Magyarkajszi it was 1.3.
In the average of fruit bearing shoots on sweet cherry, cv. Bigarreau Burlat (1.10 bud/cm) and cv. Germersdorfi 45 (0.61 bud/cm) had the largest and the lowest flower bud density, respectively. Among the fruit bearing shoots, the largest flower bud density was in the group of 0-10 cm fruiting laterals. Among cultivars, cv. Bigarreau Burlat had the largest bud density. In the groups of n- i 0 cm, 10-20 cm, 20-30 cm and 30-40 cm fruiting laterals, the lowest flower bud density was for cv. Linda, cv. Germersdorfi 45, cv. Ferrovia and cv. Sunburst, respectively. On cvs. Van and Bigarreau Burlat, large numbers of double-set flower buds were observed on the fruit bearing shoots longer than 20 cm. Fruit setting differed on the different types of fruit bearing shoots, with the lowest value measured on above 40 cm shoots. The highest fruit setting was observed on cv. Katalin, while the lowest value was measured on cv. Germersdorfi 3.
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Honeybee (Apis mellifera L.) visitation at the flowers of quince cultivars (Cydonia oblonga Mill.)
95-102.Views:185Studies were made on the bee visitation of 6 quince cultivars and on the foraging behaviour of honeybees at quince flowers for 3 consecutive years. The bee visitation was highly intense because both the plantation and its surrounding was overpopulated by honeybee colonies. Some 5.5 bee visits were counted in average at 50 opening quince flowers in 10 minutes and some 9.7 flowers were visited of the 50 ones observed meanwhile. This equals some 7 bee visits per flower per day per in good weather. Bee visitation, however, was variable and it was greatly different in the three consecutive years with fairly favourable weather. Some cultivars tended to be more and others less visited by honeybees than the others but these differences were not consequent at each occasion. No consequent relationship between the weather and the bee visitation of quince trees could be recognised. It was concluded that .other factors were responsible for the variable nature of the bee activity at quince. Most honeybees tended to collect pollen (51.6% in average for the 3 years) and usually much less gathered for nectar only (19.9%), and the rest of them were mixed behaviour foragers gathering for both (28.5%). There were some slight differences in the foraging behaviour of honeybees at some cultivars but these differences were not always consequent in consecutive years. Also the nectar production of flowers failed to affect the bee visitation and the foraging behaviour of honeybees definitely. For the variable nature of bee visitation and bee behaviour at quince flowers, supplementary pollination is needed to achieve as high set of fruits as high is required to a good crop at quince (at least 20-25% because the flower density is low of this fruit tree species). Since the intensity of bee visitation at the flowers is the only reliable estimate of the necessity of supplementary bee pollination further research is needed to explore the relationship between the number of honeybee visits and the consequent fruit set at quince.
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Determination of chlorophyll content in case of peach leaf curl disease (Taphrina deformans) with spectral analysis
49-52.Views:372One of the most important conditions of high quality fruit production is the early detection of hytopathologycal infections. The most dangerous disease of peach (Prunus persica) is the Taphrina deformans, which causes serious damages mainly in the years where the weather condition is cool and wet. In this study, healthy and naturally diseased leaves by peach leaf curl were investigated in the laboratory of University of Debrecen, CAAES, Institute of Water and Environmental Management. Both of hyperspectral measurement and the values of spectrophotometer shown the chlorophyll content of diseased leaves were lower than the healthy samples. Based on the experiments the water status of investigated leaves established the infected leaves contained more water in their tissues.
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Carotenoid composition and content in products of sea buckthorn and peach as determined by high-performance liquid chromatography
69-74.Views:385A study was conducted to analyse the carotenoids by high-performance liquid chromatography (HPLC) using reversed-phase cross linked end-capping and to study the pigment content and composition in sea buckthorn products as well as in freshly harvested fruits from peach thees grown under organic and integrated farming conditions. It was found that carotenoids in fruits of both crops occur mainly esterified with fatty acids in form of mono- and di-esters. The major carotenoids were esters of zeaxanthin, lutein, β-cryptoxanthin and β-carotene. The major carotenoids showed different response to processing of sea buckthorn being highly sensitive to thermal treatments such as blanching, cooking and drying. Significant differences were found between peach varieties in their carotenoid content, whereas the impact of organic farming on carotenoids formation was found to be variety-dependent. Two of the three varieties examined in this work, when cultivated under organic farming conditions contained lower carotenoid level as compared to that found in the fruits of the same varieties but produced in integrated farms.
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Organoleptic evaluation of pear varieties grown at different sites
33-37.Views:290It was stated as a summary that a system of testing pear varieties on scale of 100 degrees, weighing 11 properties, gives a comprehensive information on qualities of pear varieties. The points of the form to be filled out, distinguishes the appreciation of accessory flavours and tastes from the main properties. Under the title of taste, sweet and acid taste are evaluated separately. The presently used system is a step forward as the composed characters do not influence the judgement of each other. In the representation of results, the 100 degree system was more efficacious than the usual 5-point system because there was no need of using fractions, and the graphic profile is more expressive, so the commodity is better visualised. The assembly of the jury was divided into two groups as below and above 35 year old persons. The two groups judged differently the acidity, sweetness and the thickness of the fruit peel. It was suggested that the choice of the produce should consider the differences in taste and give up the idea of being equally favourable for both groups. The samples of different growing sites revealed that the best quality is produced at Nagykanizsa, and this capacity would be able to supply the market also in the large super-markets and chain of department stores in Hungary.
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First selections of the Hungarian apple breeding program for multiple resistance
9-13.Views:168The aim of the first Hungarian apple breeding program for multiple resistance started in the beginning of the nineties is to widen Hungarian apple assortment by good quality, resistant apple cultivars with excellent productivity and ecological capability to the most important fruit growing areas in Hungary. In the first years of seedling production we made early selection for susceptibility to apple scab in greenhouse. Alter this, field observation of susceptibility to powdery mildew, scab and canker and a yearly negative selection was carried on. From 1997, fruit quality was evaluated as well, and from 2001 the resistance of shoots to Erwinia ainylovora (Burrill/Winslow et al.) was examined using inoculations in greenhouse conditions. From the progenies of crosses in 1992 and 1993, six candidates were announced to national recognition out of hybrids examined for more than a decade. Descriptions of these selections from 'Prima' progenies and the most important data of their resistance, growing habit, morphological characteristics and fruit quality are shown in this article.
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Floral biology and fertility in peaches (Review article)
9-14.Views:208Floral biology and fertility in peaches (Review article)
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Integration of terrestrial laser scanning and spectral canopy scanner in horticulture applications
15-18.Views:286One of the most difficult challenge in the everyday practice to describe the canopy growing of fruit trees in an orchard. The photosynthetic activity is the basic of the primer production of plants. The measurement of leaf area and determination of the photosynthetic activity could be occurred with some elaborated methods between experimental conditions. In this article we present such an integrated methodology, which is ideal to determine the geometric and spectral characteristic of fruit trees between field conditions.We have carried out laser scanning technology to investigate the geometric-topological characteristics and parallel the active infra-red sensor to collect spectral data about an apple orchard. The surveys were worked out in an intensive apple orchard with drip irrigation system, protected by hail net in Study and Regional Research Farm of the University of Debrecen near Pallag. This study shows the filtering and interpretation methods of created data. The produced high accuracy data can be directly used in the precision horticulture. It could serve as a guiding data to implementation a future “virtual horticulture”. Higher spatial and temporal resolution could help for a better recognition of water balance of orchards.
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Hazardous element content and consumption risk of 9 apricot cultivars
61-65.Views:309The heavy metals pollution is one of the problems that arise due to the increased uses of fertilizers and other chemicals to meet the higher demands of food production for human consumption. In order to assess possible health risk of apricot (Prunus armeniaca L.) consumption, levels of Arsenic, Cadmium, Mercury and Lead were determined in fresh and dried samples of "Jumbo cot", "Tom cot", "Gold strike", "Gold bar", "Bergeron", "Bergarouge", "Sweet cot", "Yellow cot" and "Zebra" apricot cultivars. Wet digestion of samples with concentrate HNO3 – H2O2 digester mixture and inductively coupled plasma–atomic emission spectroscopy was used. Highest content of As, Cd, Hg and Pb among all cultivars, were 0.5, 0.04, 1.5 and 0.5mg/kg of dried apricot samples. Fresh fruit samples also contain 0.2, 0.016, 0.6 and 0.2 mg/kg of Arsenic, Cadmium, Mercury and Lead respectively. Daily intake of metals, hazard quotient and health risk index to reveal health risk possibility of dried and fresh fruits consumption were calculate and compared.
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Analyses of Hungarian sour cherry germplasm with simple sequence repeat markers
27-31.Views:326Twenty-four sour cherry cultivars (genotypes), belonging to four cultivar groups were fingerprinted using microsatellite markers. All genotypes have been arisen from the Carpathian basin, which could be secondary gene centre of sour cherry, since its progenitor species, ground cherry and sweet cherry overlap here. Five SSR primer pairs, earlier used for fingerprinting Turkish sour cherry germplasm were tested. None of the five primer pairs showed any polymorphism within the cultivar groups. The primer pairs were able to distinguish between the cultivar groups. The Oblacsinszka and the Cigánymeggy cultivar groups were the most difficult to separate, while the Pándy cultivar group was the most distinguishable.