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The impact of different fertilization methods on some microbiological soil characteristics
Published December 21, 2008
119-126

In our experiment, we studied the impact of an organic fertilizer, Bactofil® A10 (half- and full dosage applied in field practice) and an artificial fertilizer of Ca(NO3)2 content in different dosages (20-40 mg kg-1) – in addition to control treatments – on two different soils (calcareous chernozem, humus sandy soil) in 2005-2006, the expe...riment was complemented with treatments applying 250% dosage (100 mg kg-1 N, Bactofil® A10 2.5 times the field dosage) and a compost from urban sewage (25 g kg-1 compost) was also tested on these two soil types. In the
experiment, several soil microbial parameters were studied. The experiment was set up at the Department of Agrochemistry and Soil Science using 1-kg pots.
Our laboratory experiments were performed at the soil microbiology laboratory of UD CAS Department of Agrochemistry and Soil Science, the total number of bacteria, microscopic fungi, nitrifying and aerob cellulose-decomposing bacteria were determined together with the CO2-production of soil, N content of the biomass and urease enzyme activity.
Statistical analysis of the data was done using the program SPSS 13.0, means of the measurements, deviation and significance values were calculated. 
In 2005-2006, the effect of the different dosages of Bactofil® A10, and the Ca(NO3)2 fertilizer on the examined microbial parameters of calcareous chernozem and humus sandy soils can be summarized as follows:
• Concerning the total number of bacteria, both treatments were effective on calcareous chernozem soil, the higher (significant) increment in bacteria number was observed in the artificial fertilizer treatments, while in the humus sandy soil Bactofil treatments had a beneficial effect. The number of microscopic fungi also increased in both treatments, higher numbers were observed in the average of two years in the Bactofil treatments.
• The number of nitrifying bacteria was 2.5 times higher in both high-dosage treatments on calcareous chernozem soil, while on humus sandy soil a slight (not significant) increment was observed only int he high-dosage Bactofil treatment. The amount of aerob cellulose-decomposing bacteria significantly increased on calcareous chernozem soil in both the highdosage artificial fertilizer and the small-dosage Bactofil treatment, however, on humus sandy soil no significant increase was observed in either treatment.
• The CO2-production increased in both soil types, although it was not significant in either treatment. A higher (though not significant) soil respiration was observed in the Bactofil treatments in both soil types.
• The microbial biomass N values were significantly higher in the high-dosage Bactofil treatments, however, the high-dosage artificial fertilizer treatment also increased these values significantly on calcareous chernozem soil.
• On calcareous chernozem soil, urease activity was significantly increased and reduced by high-dosage artificial fertilizer treatments and Bactofil treatments, respectively. On humus sandy soil, urease activity was also reduced except for the high-dosage artificial fertilizer treatment. In 2007, the pot experiment with 250% dosages was complemented with the application of compost rich in organic matter, the results of these treatments are sumnmarized as follows:
• In the case of the total number of bacteria, all three treatments resulted in a significant increase on calcareous chernozem soil with the highest values in the Bactofil treatment. The Bactofil treatment was the most effective on the humus sandy soil, but the artificial fertilizer treatment also
resulted in a significant increment. In the case of the total number of fungi, Bactofil treatments resulted in the highest values on both soils, but the compost treatment also increased the number of fungi in calcareous chernozem significantly. 
• The number of nitrifying bacteria was increased most (significantly) by the Bactofil and compost treatments on both soil types. The amount of cellulose-decomposing bacteria was significantly increased by he compost treatment on calcareous chernozem soil, while its effect was not significant on humus sandy soil. The number of these bacteria was increased significantly by the Bactofil treatment on humus sandy soil.
• On calcareous chernozem soil, all three treatments significantly increased CO2-production, while the compost treatments had the resulted in the largest increment in soil respiration on both soil types.
• The soil biomass N content was significantly increased in both soils by the compost treatment, while in the case of the humus sandy soil, the Bactofil treatment also resulted in a significant increment.
• Urease enzyme activity was significantly increased by the artificial fertilizer treatment on both soils. In calcareous chernozem soil, the Bactofil treatment resulted in a slight (not significant) reduction in enzyme activity. In humus sandy soil, the Bactoful treatment also resulted in a slight reduction, while the compost treatment increased (though not significantly) the urease activity.
Based on our results, it can be stated that all three treatments were effective with respect to the studied soil microbial parameters. For both the calcareous chernozem and the humus sandy soil, the organic fertilizer Bactofil and the compost with high organic matter content had a stronger effect on some soil microbial parameters than the artificial fertilizer.

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Statistical comparison of soil analysing results of chernozem soils
Published October 10, 2008
93-99

The soil fertility was degraded as a result of unreasonable tillage, therefore the application of site-specific nutrient replacement is necessary. It is essential for the application of precision fertilization to know the location, extension, soil properties and nutrient-supply of the different soil types of
cultivated areas.
We collected... soil samples from 580 hectares of land in 2006. Soil samples were collected from every 5 ha in 30 and 60 cm depths during Spring from 20.05.2006 to 12.06.2006 and again in Autumn from 09.19.2006 to 02.10.2006. Soil samples were analysed at the Department of Agricultural Chemistry and Soil Science of DE-ATC.
The two examined soils are slightly calcareous, weakly saline, poor in zinc. The calcareous chernozem soil is slightly acid, the content of humus, nitrogen, phosphorus and potassium is medium in this soil. The meadow chernozem soil is slightly alkaline, and properly supplied with humus and potassium, and middling supplied with nitrogen and phosphorus. The meadow chernozem soil is more heterogenous in soil plasticity, lime, saline, nitrogen phosphorus and potassium content and less heterogenous in pH and zinc content than the calcareous chernozem soil.
Standard deviation of measured values in pH, soil plasticity, humus and nitrogen content significantly differ between the examined soil types. The soil plasticity, pH, humus, nitrogen and zinc content significantly differ among calcareous chernozem soil and meadow chernozem soil, but the difference in phosphorus content can be statistically proven only in case of Spring soil sampling.

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Examination of CO2 emission of different stubbles on a chernozem soil
Published November 3, 2010
53-59

Applying alternative soil cultivation methods based on reduced disturbance of the soil more favourable conditions can be created in order to increase the organic matter content of the soil and the availability of the nutrients for the crops. In complex soil tillage experiment – in 1997 was set on – at Karcag, as the element of the investiga...tion of soil reduced and conventional tillage systems. There is close correlation between the degree and intensity of CO2-emission from the soil and the structural state and organic matter content of the soil. In order to quantify the increased CO2-emission from soil due to soil preserving cultivation systems, in situ CO2-emission of soil was measured by means of an ANAGAS 98 infrared gas analyser. The soil type of the investigated plot is meadow chernozem solonetz in the deeper layers, a soil type that is characteristic
for the Trans-Tisza Region of Hungary. In this paper the results gained from the measurement on different stubbles are published, as we consider stubbles the most suitable state when the effects of different soil cultivation systems on the microbiological activity of the soil can be compared. Experimental data provided information about the length of the time period when CO2 emission increasing effects of soil cultivation are observable. Studying the effect of different soil cultivation methods on the CO2 emission from chernozem soil is indisputably actual and needs more efforts as it can contribute to develop a more environmental friendly agricultural production. The main goal of these measurements was to determine the effect of soil cultivation technologies and certain agrotechnical elements on the factors of the soil carbon cycle.

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Soil analysis as the foundation of precision nutritive supply in the Hajdúszoboszló region
Published July 16, 2007
141-148

Larger cultivated plots are heterogeneous from a pedological aspect. Heterogeneity causes problems during fertilization and harvest. The heterogeneity of cultivated areas can be compensated by fertilization which is based on soil analysis. We carried out research into the changes of the soil on three soil types, from 1966 to 2006, on the cultiv...ated areas of Hajdúszoboszló.
There were no significant changes in pH on chernozem meadow soil and meadow chernozem soil, but the pH increased in 0-30 cm layer on type meadow solonetz soil. The saline content decreased in every examined soil type. Decrease was the largest on meadow solonetz soil. Decline of humus content was the largest (0.95%) on chernozem meadow soil, and the smallest (0.39%) on meadow chernozem soil. The nitrogen content decreased with 528 ppm in the 0-30 cm layer on chernozem meadow soil, and decreased by 186 ppm on meadow solonetz soil. Phosphorus and
potassium content increased in every examined soil types. Rise of phosphorus content was 188.9 ppm in the 0-30 cm layer on meadow chernozem soil. The potassium content rose by 153.7 ppm on this soil type. Phosphorus content increased with 70.8 ppm, and potassium content increased by 57.6 ppm from 1966 to 2006.

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Comparative examination of a bacterium preparation (BACTOFIL® A10) and an artificial fertilizer [CA(NO3)2] on calcareous chernozem soil
Published November 3, 2010
75-80

In a small-pot experiment a bacterium preparation, Bactofil® A10 and an artificial fertilizer containing Ca(NO3)2 in different dosages were studied on calcareous chernozem soil, concerning the readily available nutrient content of soil (nitrate-nitrogen, AL-phosphorus, ALpotassium content of soil, some soil microbial char...acteristics (total number of bacteria and fungi, cellulose-decomposing and nitrifying bacteria, CO2-production of soil), and the amount of the plant biomass.
The readily available nutrient content of the calcareous chernozem soil increased due to the treatments, except for the change in the soil nitrate-nitrogen content, which did not measure up to the control due to the effect of high-dosage Bactofil.
The treatments also influenced the examined microbial characteristics of the soil positively. The artificial treatments significantly increased the total number of bacteria and the number of cellulose-decomposing and nitrifying bacteria. The low-dosage Bactofil significantly increased the number of cellulose-decomposing bacteria and both Bactofil dosage significantly increased the number of nitrifying bacteria. The measure of the soil respiration grew in all treatments, but significantly only in Ca(NO3)2 fertiliser treatments.
The quantity of the plant biomass was grew in a low-dosage Bactofil and significantly in the artificial fertiliser treatments. The highest plant biomass quantity was measured in the high-dosage artificial fertiliser treatment.
In the correlation analyses we found some medium positive correlation between the soil chemical, microbiological parameters examined, and the plant biomass in the case of both treatment-forms. 
Based on our results Ca(NO3)2 artificial fertiliser treatments on calcareous chernozem soil proved to be more stimulating regarding the
examined soil characteristics and the amount of the plant biomass, but the low-dosage Bactofil also positively influenced the majority of the
soil characteristics examined in terms of nutrient supply.

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Comparative analysis of soil analysing datas on different sempling-plots
Published May 23, 2006
85-90

Hibrid maize is cultivated on larger plots, therefore the sown areas of hibrid maize are heterogeneous from a pedology aspect. Heterogenity causes problems during tasseling, chemical plant protection and harvest. The heterogenity of sown areas can be compensated by fertilization which is based on soil analysis. We carried out research into chan...ge of the soil on four soil types from 1987 to 2005.
There were no significant changes in pH, hydroiodic acidity, CaCO3-content, humus-content on meadow chernozem soil. We detected equalization of salin content in the examined soil layers. There were no significant changes in the measured values on chernozem meadow soil and solonetz meadow soil in 2005. We discoverd equalization of saline content on chernozem meadow soil, but the changes were not as obvious as the changes on meadow chernozem soil. We found salinization in the 30-60 cm soil layer on type meadow soil that may be due to water movement.

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Changes of some soil chemical and microbiological characteristics in a long-term fertilization experiment in Hungary
Published September 5, 2018
253-265

Agricultural management practices – directly or indirectly – influence soil properties.

Fertilization rates and crop rotation can strongly affect soil pH, soil nutrient supply and soil organic matter content due to the changes of microbial processes. The objective of this study was to compare the effects of different fertilization d...oses in monoculture and tri-culture of maize (monoculture: only maize grown since 1983, tri-culture: it is a three-year crop rotation system: pea – winter wheat – maize) on selected soil characteristics. The long-term fertilization experiments were set up in 1983 in Eastern Hungary. These experiments are situated west of Debrecen in Hajdúság loess region, on calcareous chernozem (according to WRB: Chernozems).

The test plant was maize (Zea mays L.). One-one pilot blocks were selected from monoculture and tri-culture of the long-term experiments. The observed soil samples were taken in the 30th year of the experiment, in 2013. The doses of NPK fertilizers increased parallel together, so the effects of N-, P- and K-fertilizers cannot be separated.

With the increasing fertilizer doses, the soil pH has decreased in both crop production systems and, in parallel, the hydrolytic acidity has significantly increased. A close negative correlation was proved between the pHH2O, pHKCl and hydrolytic acidity. An increased nutrient content in soil was recorded in every NPK treatment and the available phosphorus and nitrate content increased in higher proportion than that of potassium. Of the measured parameters of C-and N-cycles, fertilization has mostly had a positive effect on the microbial activity of soils. Besides the effects of fertilizer doses, correlation were looked for between soil microbiological properties. Evaluating the ratios among the measured parameters (organic carbon and microbial biomass carbon, OC/MBC ratio; carbon-dioxide and microbial biomass carbon; CO2/MBC proportion), the fertilization rate seems to be favoured by the increase of amounts of organic compounds

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The effect of nickel-contamination, nitrogen-supply and liming on the chemical composition of perennial ryegrass (Lolium perenne L.)
Published October 10, 2008
85-92

Plant-production is determined by many production-factors. Each of these factors became subject of research-works through the years, still we state, that studying their interaction is even more important. For studying these interactions we set up a potexperiment, within that the direct effect and the interactions of four factors was inspected: ...soil, nitrogen-supply, nickel-loading and liming. Experiments were carried out on two soil types with extremely different characters: one was a chernozem soil with good fertility and buffering capacity, the other was a shifting sand soil with low humus-content and buffering capacity. Nitrogensupply and liming was added on two levels, while nickel on three within 12 combinations on each soil types. Plant production was cut two times within the vegetation period. The amount of production and dry matter was weighted, fractured and their element-content was measured by an ICP-detector.
Ca-content on the shifting sand soil was determined by all three factors, however the interaction between nickel-loading and liming was also significant. Nitrogen and liming increased Cauptake, that is due to appropriate nutrient-supply and improvement of a better pH-value. On the chernozem soil nitrogen and CaCO3 also increased the Ca-content. This is caused by a better nutrient supply and a higher amount of available Ca-ions.
On the shifting sand soil nickel content was increasing parallel to higher nitrogen-dosages. In presence of higher nickelamount the nickel-content of plants was also increasing, still according to liming, this increment was different. On the chernozem soil nitrogen a nickel increased Ni-uptake. However, liming also had a positive effect on Ni-content, that can be explained by the high amount on colloids in the soil, the adsorption of Ni-ions on them and in presence of liming material the replacement of Ca-and Ni-ions. 
The potassium-content on the shifting sand soil was different in each liming-combination. In combinations without nickel the potassium-content of limed and not limed combinations was on the same level. In not limed combinations by adding nickel potassiumcontent was increasing, while in limed combinations no change was observed. On the chernozem soil by adding liming material the amount of uptaken potassium was decreasing, that is due to the antagonism between Ca- and K-ions. 

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Effects of cultivation methods on some soil biological parameters of a meadow chernozem soil (Vertisols)
Published November 3, 2010
61-66

The effect of extended drought conditions on soil, the unfavourable cultivation technologies and the application of chemicals have been enhancing the processes of physical and biological soil degradation, so the fertility of soil is gradually declining. 
The effects of two cultivation methods – traditional ploughing (TP) and conservat...ion tillage (CT) – on the biological activity of a meadow
chernozem soil were examined in a long term experiment. Different parameters of the biological activity of soil were determined. These are
the numbers of total bacteria, microscopic fungi, aerobic cellulose decomposing bacteria, as well as the activities of some important soil
enzymes and CO2 production.
Conservation tillage seemed to be a more favourable cultivation method for the majority of microorganisms, the activities of urease and
dehydrogenase enzymes and CO2 production, compared to the traditional ploughing system. These parameters increased significantly,
especially in the upper layer of conservation tillage plots. Concerning the plant cultures, the majority of microbiological parameters were
higher in the soil of vetch (Vicia sativa L.) depending on the cultivation methods, so involving the pulses to the crop-rotation seems to be
very important in this soil type.
According to the ninth year’s results, the importance of conservation tillage as a means of protecting the soil biological activity in meadow
chernozem (Vertisols) can be established; it was proven by microbiological investigations.

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Effect of PRPSOL soil conditioner on the physical status of the soil in conventional and reduced tillage systems
Published February 25, 2014
109-113

The effect of PRP-SOL soil conditional on soil compaction, moisture content and bulk density is studied in a long-term soil cultivation experiment from 1997 on a heavy textured meadow chernozem soil, in reduced and conventional tillage at Karcag Research Institute. Our investigations were made in the vegetation period of corn, in June and after... harvesting, on stubble. Soil compaction was measured with a penetrometer, the actual moisture content was determined by gravimetric method. The bulk density values of the regularly cultivated soil layer of 0–10 and 10–20 cm depths were defined from undisturbed soil samples. We established that after 3 years the application of the soil conditioner has positive effect on soil compaction and moisture status of the top layer in the reduced tillage system. We could not figure out this positive effect in the case of conventional tillage.

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Nitrogen Content of Hungarian Soils and Nitrogen Fertilization
Published December 10, 2002
51-61

For crop production and agricultural production, the most important natural resource is the soil that can optionally renew. Paralelly with this, soil plays a major role in the geological and biological cycle of elements. As a result of the big (geological) and small (biological) element cycles, the elements and combines neccessary for organisms... can accumulate in the soil creating suitable living conditions for plants and other organisms. Soil is a heterogenous system both horizontally and vertically, and soil constituents show great variety in all the three dimensions, in addition, most of the parameters can also change between two examination dates. When talking about the factors influencing plant production, one should take into account this variation and heterogenity in time and space. When making fertilization recommendations, these factors should all be considered. In any consultation system, most of the mistakes and errors made are due to the unsatisfying soil testing and the negligence of soil heterogenity. In the practice of fertilization the biggest mistake is the improper soil sampling, then comes the methodical mistake of soil testing, which is followed by the inaccuracy of instrumental analysis and the subjectivity of result evaluation, but the latter two are negligible compared to the others. Under normal, i.e. production conditions, the quantity and distribution of nutrients in the soil are greatly dependent upon the applied technology, the amount and form of the applied natural and artificial fertilizers and the quality of fertilization.
Fertilization recommendations are needed because in the layer which is accessible for plant roots only a part of the nutrient content is available for plants in a specific production cycle. An illustration of this is that though the upper 1 m layer of an average chernozem soil contains more than 5000 kg N, 12000 kg K2O and 1500 kg P2O5 (form of expression mostly used in Hungary), the application of fertilizer doses which are just fractions of these quantities is essential. This is due to the fact that the available amount of the total nutrient content depends from the quality of soil, the environmental factors (the physical and chemical qualities of the soil) and the specific nutrient’s qualities (solubility, adsorption). Knowledge of these processes and the examination of the factors influencing the actual nutrient content are vital for working out a fertilization practice, which does not put more strain on the environment than neccessary.

All of the above mentioned should be considered when applying inputs in the fields. In a well-functioning practice that considers the economic and environmental conditions (unfortunately the present production and economic conditions do not enable an appropriate level and degree), three nutrients are supplemented generally (and were supplemented in the last decades): nitrogen, phosphorus, potassium.
Studying the nutrient balance of the Hungarian field production’s last hundred years, we can draw some interesting conclusions.
The nutrient balance became positive for nitrogen and potassium in the second half of the 1960’s, while for phosphorus it was positive from the first half of the 1960’s and this period lasted until the end of the 1980’s.
Neither before the 1960’s, nor since the 1990’s has the amount of nutrients supplemented in a specific year reached the amount of the nutrient uptake of the same year.

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Examination of the physical state of the soil under conventional and reduced tillage systems
Published February 10, 2013
183-186

...5); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">he effect of reduced and conventional tillage systems on soil compaction and moisture content in two years with extreme weather conditions is introduced in this paper. The investigations were carried out in a long-term soil cultivation experiment set on a heavy textured meadow chernozem soil at the Karcag Research Institute. In 2010 the amount of precipitation during the vegetation period of winter wheat was 623.3 mm, 2.2 times higher than the 50-year average, while in 2011 this value was 188.7 mm giving only 65% of the average. The examinations were made after harvest on stubbles on 4 test plots in 5 replications in the case of each tillage system. Soil compaction was characterised by penetration resistance values, while the actual soil moisture contents were determined by gravimetry. The values of penetration resistance and soil moisture content of the cultivated soil layer were better in the case of reduced tillage under extreme precipitation conditions. It could be established that regular application of deep soil loosening is essential due to the formation of the unfavourable compact soil layer under 30 cm. Conventional tillage resulted in enhanced compaction under the depth of ploughing, the penetration resistance can reach the value of 4 MPa under wet, while even 8 MPa under dry soil status.

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Impact of ammonium nitrate and Microbion UNC bacterial fertilizer on dry matter accumulation of ryegrass (Lolium perenne L.)
Published November 3, 2010
35-39

Pot experiment was performed to investigate the effects of increasing NH4NO3 doses with or without Microbion UNC bacterial fertilizer
application on dry matter production of ryegrass (Lolium perenne L.). Experiment was set up on calcareous chernozem soil of Debrecen-Látókép and on humus sandy soil of Őrbottyán. The bi-factorial trials w...ere arranged in a randomized complete block design with four replications. Grass was cut three times. Dry matter production was determined and the sum of biomass of cuts was calculated as cumulated dry weights. Analysis of variance was carried out on the data in order to provide a statistical comparison between the treatment means. The least significant difference (LSD5%) test was used to detect differences between means. On the basis of our results it can be concluded, that the dry weights of ryegrass cultivated on chernozem soil were higher than on sandy soil. With increasing nitrogen supply the dry matter production of grass significantly increased in both types of soils. In case of sandy soil the increasing effect was more expressed, but dry weights of this soil never reached the appropriate values of chernozem soil. Application of Microbion UNC had positive effect on dry matter production of ryegrass grown on both two types of soils but the effect was more expressed on chernozem soil. Finally it can be concluded that the increasing effect of NH4NO3 on biomass weights was more expressed in both types of soils, the biofertilizer application also increased the dry weights of plant in a small degree. 

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Studies of the influences of different N fertilizers and Microbion UNC bacterial fertilizer on the nutrient content of soil
Published October 5, 2010
134-140

A field experiment was conducted to examine the effects of different nitrogen fertilizers in combination with bacterial fertilizer on
nutrient uptake of horseradish and plant available nutrients of the soil. Three different N fertilizers, ammonium-nitrate, urea and calciumnitrate
(116 kg ha-1 N) in combination with Microbion UNC bacterial... fertilizer (2 kg ha-1) were applied as treatments in a randomized
complete block design in three replications. In this paper we presented the results of soil measurements. The soil of the experimental area
was chernozem with medium sufficiency level of N and P and poor level of K.
Our main results:
The amount of 0.01M CaCl2 soluble inorganic nitrogen fractions, NO3
--N and NH4
+-N and also the quantity of soluble organic-N were
almost the same in the soil. N fertilizers significantly increased all the soluble N fractions. The amount of NO3
--N increased to the greatest
extent and the increase of organic N was the slightest. We measured the largest CaCl2 soluble NO3
- -N and total-N contents in the plots
treated with ammonium-nitrate, the largest NH4
+-N in the plots treated with calcium-nitrate and the largest organic-N fraction in plots
treated with urea.
Bacterial inoculation also increased both soluble inorganic nitrogen forms and also total-N content of soil compared to the control. In
the case of combined (artificial and bacterial fertilizer) treatments we measured lower NO3
--N, organic-N and total-N compared to the
values of plots having only nitrogen fertilizer treatments. On the contrary in the plots with combined treatments the CaCl2 soluble NH4
+-N
content of soil in more cases were higher than that of values with artificial fertilizer treatment.
As a function of calcium-nitrate application increased AL-P2O5 and AL-K2O values were measured compared to control. Microbion
UNC supplement of calcium nitrate yielded also increase in AL-P2O5 and AL-K2O values, till then supplement of ammonium-nitrate fertilizer
yielded a decrease in these values compared to the control.
All nitrogen fertilizers resulted in a significant decrease in AL-Mg content of soil compared to the control. Nevertheless bacterial
fertilizer increased AL-Mg values in any cases.

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The effect of different microbial preparations on some soil characteristics
Published July 31, 2012
83-86

In pot experiment the effect of different microbial inoculants and their combinations with NPK fertilizer and wheat straw on some soil properties (physical, chemical, and microbiological parameters) were studied. The experiment was set up in 2011 at the Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random b...lock design. The studied soil type was calcareous chernozem soil from Debrecen (Látókép) with ryegrass (Lolium perenne, L.) test plant.
At the end of the experiment in our laboratory the nitrate-nitrogen content of soil, the AL-soluble phosphorus and potassium content of soil, the urease enzyme activity of soil, the total number of bacteria and the number of microscopical fungi were determined.
The results of the study were the following:
– The straw treatment and the straw + biofertilizer combinations influenced positively the nitrate content of soil.
– The NPK fertilization and the straw+bacterial fertilizer combinations had significant positive effect on the AL-soluble phosphorus content of the soil.
– The biofertilization and the straw+biofertilizer combinations stimulated the AL-soluble potassium content of soil occasionally.
– The total number of bacteria was influenced by the NPK fertilization, the bacterial fertilization and the straw+bacterial fertilizer combinations significantly.
– In case of the number of microscopic fungi caused in some cases significant changes the NPK+bacterial fertilizer and straw+bacterial fertilizer combinations.
– The soil urease enzyme activity was increased in all cases strongly especially by the straw+bacterial preparation combinations.

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The effect of different bacterial fertilizers on the AL-soluble P2O5 content of soil, and the biomass of the rye-grass (Lolium perenne, L)
Published December 16, 2012
93-98

In pot experiment the effect of different bacterial fertilizers on some soil properties, and the amount of plant biomass were studied. The
experiment was set up in 2010 at the Department of Soil Science and Agricultural Chemistry, in a three replications in a random block design. The ryegrass (Lolium perenne, L.) was used as a test plan...t. The studied soil type was calcareous chernosem soil from Látókép. In our laboratory AL-soluble P2O5 content of soil, the phosphatase enzyme activityof soil, the dry weight of rye-grass, and the phosphorus content of rye-grass were determined.
The results of the study were the following:
– The bacterial fertilizers - by basic treatments NPK - had significant positive effect on the AL- soluble phosphorus content of the soil.
– The soil phosphatase enzyme activity was increased in all cases strongly by the microbial preparations used, the greatest impact was the Bactofil A bacterial fertilizer.
– The plant educed P values significantly increased by the effect of microbial products, in addition to the fund NPK. In this case, the EM-1 and Microbion UNC bacterial fertilizer were the effective.
– In case of the rye-grass biomass none of the bacterial preparations used caused any significant changes, either alone or when used them with straw treatment.

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Evaluating changes in nitrogen and sulphur content in a soil-plant system in a long-term fertilization experiment
Published December 1, 2020
77-85

The objective of this study was to evaluate the impact of long term NPK fertilization (considering that S containing superphosphate was supplied for 26 years of experiment, but since 9 years S has not used any longer) on sulphur- and nitrogen content and N/S ratio of winter wheat. The second objective of this work was to determine the chang...es of the amount of the different nitrogen and sulphur fraction in chernozem soil in a long term fertilization experiment. The third aim of the work was to determine if a relationship could be established between the studied parameters. Based on our results, it can be stated that the sulphur containing superphosphate supplied in the period of 1984-2010 has no longer significant effect on total sulphur content of plant in 2018. The NPK fertilization treatments had positive effect on total nitrogen content of winter wheat. In general, increasing NPK doses resulted in significantly higher nitrogen. The effect of irrigation applied in previous years has no statistically significant effect on the sulphur and nitrogen content of wheat. The wheat grain produced in our experiment, especially in fertilized treatments showed S deficiency. Analysing the changes of CaCl2 soluble nitrate-N and total N of the soil, it can be stated that the effect of increasing fertilizer doses clearly appears in these parameters, because the treatment with increasing fertilizer doses resulted higher CaCl2 soluble N forms compared to the control treatment in soil. These values increased until flowering stage of wheat and after that a slightly decrease was observed as a result of higher N uptake of plant. In overall, it can be stated, that the effect of superphosphate on measured sulphur fraction is prevailed. With increasing fertilizer doses higher sulphate content was detected in soil, but the sulphate content measured in different soil extractant is not enough for the wheat in this experiment area. Studying the correlation between the measured parameters of plant and soil, it can be concluded, that the relationships between nitrogen in the plant and in the soil is stable, and did not change during the growing season. The correlation between plant S and soil S varied in the measured periods and the r value was low in most cases. At the stage of flowering the highest r value was found between KCl-SO4 and plant S. In the stage of ripening the strongest correlation was detected between KH2PO4-SO4 and grain S content.

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The effect of sulphur and nitrogen supply on the growth and nutrient content of spring wheat (Triticum aestivum L.)
Published June 30, 2018
65-70
Sulphur is an essential element for plants. Decreasing sulphur deposition from the air, and the use of more concentrated phosphate fertilizers, which contain no sulphur, has led to reports of sulphur deficiencies for wheat. Sulphur deficiency significantly affects yield and also the quality of wheat. The pot exper...iment was set up on calcareous chernozem soil at Látókép, Hungary, test plant was spring wheat (Triticum aestivum L). Seven treatments were used where nitrogen and sulphur were supplied as soil fertilizers in increasing rates (NS1, NS2, NS3) and in foliar fertilizer as well (NS1+fol., NS2+fol., NS3+fol.). Plant aboveground biomass production was determined in samples taken in the stages of development BBCH 29-30, 51-59, 61-69, 89. The nitrogen and sulphur content of straw and grain were measured. N/S ratios of grain and straw were calculated. The weights of grain were ranging between 8.6–16.1 g/pot. NS2 and NS2+fol. treatments produced the highest values. Foliar fertilizer had no further effect on grain. Analysing the values of the straw, it was observed that tendencies were similar to values of grain. The NS2 treatment produced the highest weight of straw and the NS3 rate already decreased that amount. The obtained results show the unfavourable effect of excessively high rate applied in NS3 treatment. The supplementary foliar fertilizer had no significant influence on the weight of straw. Both N and S-uptake of plant was very intensive at the stem elongation stage, then the N and S-content of plant continuously decreased in time in all treatments. The N-content of grain ranged between 2.215–2.838%.
The N-content of grain slightly increased with increasing of nitrogen doses. In the higher doses (NS2, NS3) foliar fertilization slightly increased the nitrogen content of grain, although this effect was not statistically proved. The N-content of straw varied from 0.361 to 0.605%. The growing dose of soil fertilizer also considerably increased the nitrogen content of straw. Foliar fertilization further increased the nitrogen content of straw. The S-content of grain ranged between 0.174–0.266%. The lowest fertilizer dose (NS1) significantly increased the sulphur content of grain. The further increasing fertilizer doses (NS2, NS3) did not cause additional enhance in sulphur content of grain.
The foliar fertilizer also did not change the sulphur value of plant. The increasing amount of soil fertilizer and the supplementary foliar fertilizer had no effect on the sulphur content of straw. The treatments influenced the N/S ratios of grain and straw. On the basis of experimental results it can be concluded that the examined nitrogen and sulphur containing soil fertilizer had positive effect on the growth and yield of spring wheat grown on the calcareous chernozem soil. The soil fertilizer application enhanced the grain nitrogen and sulphur content. The highest rate of fertilizer (600 kg ha-1) proved to have decreasing effect on the yield. The sulphur and nitrogen containing foliar fertilizer did not have significant effect on the yield parameters but slightly increased the nitrogen content of plant.
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Evaluation some important microbiological parameters of the carbon cycle in chernozem soils profiles
Published October 24, 2016
33-39

Some chemical and microbiological properties of the carbon cycle were investigated in three chernozem soil profiles. The soil profiles originated from a long term fertilization experiment (potato) of the University of Debrecen, Látókép, Kryvyi Rig Botanic Garden (grassland) and a large-scale farm (sunflower) of Ukraine. The results of the or...ganic C-content, total number of bacteria, microscopical fungi, cellulose decomposing bacteria, CO2-production, microbial biomass carbon and saccharase and dehydrogenase activities were compared and evaluated with the help of correlation analyses. Close correlation was found between the organic carbon content and the number of microscopical fungi,, saccharase and dehydrogenase enzymes’ activities, as well as close correlation was found between the dehydrogenase activity and microbial biomass-C and saccharase activity.

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98
119
Effect of different sources and doses of sulphur on yield, nutrient content and uptake by spring wheat
Published June 1, 2021
109-115

The objective of this study was to investigate the effect of two sulphur forms (sulphate and tiosulphate) in combination with three different N:S ratios on the yield of spring wheat and total N- and S-content and uptake by the aboveground biomass on chernozem and sandy soil. In the greenhouse experiment, the effects of two sulphur forms wer...e compared: sulphate (SO42-) and thiosulphate (S2O32-). The sulphate was applied as potassium-sulphate (K2SO4) and thiosulphate as ammonium-thiosulphate ((NH4)2S2O3). Increasing doses of both sulphur forms (24, 60, 120 kg S ha-1) were used with the same nitrogen dose (120 kg N ha-1) which caused three different N:S ratios background (1:0.2, 1:0.5, 1:1). Nitrogen was supplied in the form of monoammonium-phosphate (MAP), ammonium-nitrate and ammonium-thiosulphate. Plant samples were taken in three different development stages of spring wheat based on the BBCH scale: at the stage of BBCH 30–32 (stem elongation), BBCH 65–69 (flowering) and BBCH 89 (ripening). The total nitrogen and total sulphur content of plant at different development stages and also wheat grain were measured by Elementar Vario EL type CNS analyser. The nutrient uptake by plant and grain was calculated from the yield of spring wheat and the N and S content of plant.  The grain yield on chernozem soil ranged between 6.31 and 12.13 g/pot. All fertilised treatments significantly increased the grain yield compared to the control. The highest yield was obtained in the case of the application of 120 kg N ha-1 and 60 kg S ha-1in sulphate form. The grain yield on sandy soil varied from 2.53 to 6.62 g/pot. The fertilised treatments significantly enhanced the yield compared to the control. The highest yield was observed in the case of the application of 120 kg N ha-1 and 60 kg S ha-1 in thiosulphate form. On chernozem soil the increasing doses of sulphur (24, 60, 120 kg S ha-1) with the same N dose (120 kg N ha-1) increased the N-content of spring wheat at all development stages and in the grain. The treatments with different sulphur sources did not cause further changes in the N-content. On sandy soil in the most cases the N-content did not change significantly as a result of increasing sulphur doses. The treatments with sulphate form basically resulted higher nitrogen-content than treatments with thiosulphate form. The treatments with increasing sulphur doses resulted higher S-content on both of chernozem and sandy soil in the case of all development stage. Comparing the effect of the applied sulphur sources on the S-content it can be stated that at the stage of BBCH 30–31 and 65–69 the treatments with sulphate form resulted higher sulphur-content. At the stage of BBCH 89 there was no significant differences in S-content of grain as a result of different sulphur-sources.

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60
76
Investigation of soils of stubbles of winter wheat and winter peas in conventional and reduced tillage systems
Published February 3, 2016
95-99

The effect of reduced and conventional tillage on soil compaction, soil moisture status and carbon-dioxide emission of the soil was studied on a meadow chernozem soil with high clay content in the soil cultivation experiment started in 1997 at Karcag Research Institute. Our investigations were done on stubbles after the harvest of winter wheat ...and winter peas after the very droughty vegetation period of 2014/2015.

We established that the soil in both tillage systems was dry and compacted and the CO2-emission was very low. The positive effects of reduced tillage could be figured out only in the soil layer of 40–60 cm in the given weather conditions of that period.

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130
126
Relationship between the change of soil moisture content of different soil layers and maize yield
Published April 23, 2014
19-25

The development of chernozem soil water management and its relationship with maize yields was studied in a 30-years long-term field experiment with different crop-rotation systems (mono-, bi- and triculture), in three crop years with different natural precipitation: a drought (2007), a wet (2008) and a dry (2009 one. The relevant soil layer was... divided to three sub-layers: (0–60 cm, 61–120 cm, 121–200 cm) in which the development of soil moisture content was investigated during the whole vegetation. From the results it can be stated that change of the water stock of the upper soil layer (0–60 cm) was the most intensive. Both the direct effect of natural precipitation and irrigation could be observed in the most obvious way in it. Yield result of maize and the highest water supply deficit values in the vegetation were compared in our work too. According to the results it was revealed that among the three studied crop rotation systems it was the monoculture, the success of production of which depends the most of water supply. The most favourable crop rotation system was the triculture from both the aspect of the yield of produced crops and the favourable soil properties too.

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119
98
Examination the effects of different herbicides on the soil microorganisms of a calcareous chernozem
Published November 3, 2010
121-126

Pesticides play a key role in fighting weeds, pests and parasitic fungi. According to surveys, pests reduce the yield of agricultural crops by 35% worldwide. Pests, fungi and weeds account for 14%, 12% and 9% yield loss, respectively (Gáborjányi et al., 1995). Chemicals have contributed to increasing and maintaining the yields of crop product...ion for decades. Today, agricultural production (in spite of many efforts) is unthinkable without the use of pesticides (herbicides, insecticides and fungicides). On the other hand, these chemicals contribute to the pollution of the atmosphere, surface and underground waters, and agricultural soils, especially if they are applied improperly.
The sustainable agricultural production pays attention to environment-friendly cultivation-technologies; but at the same time it makes an effort to produce good quality and economical products. The examination of the herbicides’ secondary effects, fits into this chain of idas namely, how the herbicides affect – stimulating or inhibiting – the soil microbiological processes, prevention of soil fertility.
In the course of the experimental work the effect of herbicides on soil biological properties were examined in different maize (Zea mays) cultures. We wanted wished to know that how the herbicides affect the quantity change of soil microorganisms, the life of different physiological groups of bacteria and the activity of microorganisms. A small pot experiment was set up in 2008 with the application of two herbicides - Acenit A 880 EC and Merlin 480 SC – in the breeding house of the Department. The moisture content and nutrient supply were at optimal level in the experiment.
On the basis of results the following can be stated: 
1. It can be stated that the two herbicides and all their doses affected negatively the number of total soil bacteria, the
inhibiting effects were significant. The quantity of microscopical fungi increased by the effect of Merlin 480 SC and decreased in the treatments of Acenit A 880 EC.
2. The Acenit A 880 EC had stimulating effect on the nitrate mobilization. The CO2-production was stimulated by the basic doses of herbicides; the other treatments did not influence the CO2-production significantly.
3. The quantity of microbial biomass-carbon –except for only one treatment- decreased significantly by the effect of herbicides. Besides it, the quantity of microbial biomass-nitrogen increased significantly in the treatments of Acenit A 880 EC.
4. The biomass of test plant decreased in the treatments of herbicides, their quantities were smaller than in the control. In the pots treated by Merlin 480 SC, parallel with the increase of doses decreased the quantity of plant-biomass.

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Microbiological preparations affecting the soil nutrient availability and growth of ryegrass in a pot experiment
Published March 11, 2014
49-53

The effects of different bacterial fertilizers and their combinations with NPK fertilizer and wheat straw were investigated on some soil properties (chemical parameters) and on the biomass production of testplant. The applied quantities of the bacterial fertilizers were the double of the recommended dose. The experiment was set up in 2013 at th...e Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random block design. Calcareous chernozem soil; originating from Debrecen (Látókép) was used with ryegrass (Lolium perenne L.) test plant. At the end of the experiment (after 8 week) the samples of soil and plants were determined for nitrate-nitrogen, ALsoluble phosphorus and potassium content of soil, the weight of green biomass of ryegrass per pot, the dry matter and moisture content of ryegrass. Straw treatment resulted better water and available nutrient content of soil in general. Inoculation however was not improving the biomass production over the fertilizer treatment. Interrelation with the recommended dose could be further studied.

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98
109
Examination of lead absorption ability on chernozem soil and the observation of the accumulation effect of Lactusa sativa L. in pot experimentation
Published March 11, 2014
101-104

In our research a chernozem soil sample formed on loess was collected from an area under cultivation. Our aim was to determine the lead adsorption capacity using a soil column experiment. The study showed saturation of lead content of the soil. The lead accumulation capacity of Lactuca sativa L. was measured in the sections of roots and leaves ...applying pot experiments. It could be observed that the lettuce accumulated lead easily from the chernozem soil. The lead content was increased in the analyzed sections of the plants against an increasing lead content.

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