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Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
15-22Views:323Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.
Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.
Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.
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Inheritance of Plant and Ear Height in Maize (Zea Mays L.)
34-38Views:459Plant and ear height are very important characters not only for describing new varieties of maize (Zea mays L.), but for green and dry matter production, and even for grain yield. Significant positive correlations have been reported by various authors between plant height and stover yield, plant height and dry matter yield, and plant height and grain yield. The height of the main ear is also correlated to plant height. It depends on the variety or the environment, but is likely to be the same height within a population. Many environmental and agronomical factors (e.g. plant density, fertilization, pests and diseases) influence the expression of these characters, which are not quality traits. Their expression is controlled by many genes and by the interactions between these genes. The heritability of these traits is high and they show significant genotypic variability and positive heterosis, as reported in many research publications.
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Laboratory and small plot fungicide trials to control potato late blight (Phytophthora infestans) next to Sepsiszentgyörgy town (Romania)
72-72Views:127Our experiments were carried out during 2009 and 2010 years on Szépmező field, next to the Sepsiszentgyörgy town, Kovászna county, Romania. The experimental area situated at 520 - 580 m altitude, its soil is a humus rich chernozem, the yearly average temperature is between 7 - 8 oC, the precipitation is 500 - 600 mm/year. 7 different fungicides efficacy were studied for control to potato late blight disease caused by Phytophthora infestans on three potato varieties, viz. Ostara, Santé and Desirée.
In the laboratory the fluanizam (500 g/L, ALTIMA 500) fungicide was the best in inhibition of growing fungus while on the field trial plots cymoxanil + famoxadone (22.1 + 16.6%, EQUATION PRO WP*) and metalaxyl** + mancozeb (200 g/L + 1.600 g/L, RIDOMIL MZ 72WP) were the most effective. This facts were confirmed by statistic Duncan-test and data of yields.
The appearance of Phytophthora infestans epidemic was influenced by the climate conditions too during May and June when the minimum temperature was 12 oC, and the maximum 20 oC, the rain quantity more than 120 mm, the air humidity above 75%, these conditions promote the appearance and spread of fungus.
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Evaluation of mineral element content of beetroot during the different stages of the growing season
459-469Views:292In modern nutrition, bioactive materials of different vegetables are especially important to be researched. The experiment was carried out on March 30, 2016 (sowing date). Mineral element content (B, Ca, Cu, Fe, K, Mg, Na, P, S, Zn) was evaluated on the 60th, 85th and the 105th day of the vegetation period. The data are presented as the mean of five varieties which can give the real state of these parameters of beetroot grown on lowland chernozem soil.
In the young beetroot leaves (60 days) the mineral element content was higher than in the older ones (85 days). The calcium and magnesium content of the leaves was much more favourable (10 times higher) than in the root. The potassium content of leaves has reached the amount of 5000 mg kg-1, but at the same time more than 3000 mg kg-1 was detected in the improved root. This value is favourable for the potassium supply of the human organism.
The sulphur content (1300 mg kg-1) of the leaves was the highest on the 85th day of vegetation period. Similar tendency was detected for boron content (2.45 mg kg-1), while for iron content it was higher (28.23 mg kg-1) in the younger leaves (60 days).
Finally, it can be concluded that the increased element content of beetroot leaves will be a favourable source of mineral element supply of the human organism.
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Mathematical modelling of surface irrigation for field crops in Jordan based on soil hydrological-physical properties
137-148Views:106Jordan suffers from drought and depletion of water resources. In-field crop management, the issue of irrigation scheduling is important and influential. In this research note, a simple method was developed for scheduling surface irrigation of field crops based on inputs of crop ecology, effective root depth, soil texture, soil hydrology, and logical mathematics. It was concluded that the science of mathematics has succeeded to meet academic irrigation scheduling in terms of surface irrigation for field crops based on both soil hydrological and physical traits. Extension scholar has a decision to choose mathematical irrigation model depends on the traditional inputs or updating the model by searching for renewable inputs such as different varieties root depths, optimum row spacing of each crop, drip irrigation mathematical modelling, and digital sensing. In both cases, the input related to the effective root depth is a major and basic factor in mathematical irrigation scheduling. It is, therefore, recommendable that extension research-based systems should focus on basic mathematics to capacitate the complementary role of academics, research, and extension in irrigation modelling, and rural development.
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The Role and Importance of Variety in Intensive Cherry Production
81-84Views:80Increasing the intensity of plantations is a basic precondition for the renewal of fruit growing in Hungary.
The intensification of production of stone-fruit species is difficult for several reasons. In particular, knowledge of dwarfing rootstock is very limited. In this study, we tried to achieve smaller-sized, intensive crown formation by co-ordinating the date and degree of pruning.
In intensive cherry production, the most important variety-dependent characters determining the formation of the required productive surface are vigour of growth, branching potential and quantity and quality of the buds.
In this paper we have demonstrated that significant differences exist between varieties in these characters. -
Characteristics of Corn Seed Sales in Hungary
78-81Views:96As seen on the above tables we can acknowledge the fact that the Hungarian corn seed market is very unique and, because of its size, indispensable for all multinational breeder companies. We can also assume that the main places in the market are taken by big companies as Pioneer, with its unbreakable first place, followed closely by Syngenta Seeds and the fusionized Monsanto. Although the Hungarian breeders’ hibrids perform on field tests and trials similarly or better than other varieties developed by the above mentioned companies, we can’t find them even near to those leading few given mostly by Pioneer, Syngenta and Monsanto hibrids. This fact leaves us with a conclusion that the Hungarian companies should change the strategy of their marketing procedures to increase sales.Thus, they need better technology to keep up with their competitors, more advertisement, other marketing „hints”, which can only be fulfilled with a big economical background, unfortunately not yet available for our breeders.
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Evaluation of sweet sorghum and sudangrass varieties by the viewpoint of bioethanol production
57-61Views:140Bioenergy and biofuels are very important in today’s energy policy. These kinds of energy resources have several advantages against fossil fuels. Environmental protection is a cardinal point of widespreading these technologies but the economic considerations are important as well. In order to improve the rate of the renewable energy in the energy consumption, the European Union settled down a program which determines a minimum ratio of renewable energy in the energy consumption for each member country of the EU. To fulfil the requirements bioenergy and biofuels should be produced. This production procedure needs adequate stocks which are commonly agricultural products.
One of the promising stocks is sorghum. This plant fits for bioethanol production due to its juice content being rich in sugar. In this study six sweet sorghum hybrids, two sudangrass hybrids and a sudangrass variety have been evaluated to determine their theorical ethanol production capacity.
On the score of the results of the year 2009 it can be set that sudangrasses have a lower theorical ethanol capacity than sweet sorghums have. In the case of sweet sorghums 1860.29–2615.47 l ha-1 ethanol yields had been calculated, while the sudangrasses had only 622.96–801.03 l ha-1. After that throughout three years (2011–2013) the sweet sorghum hybrids have been evaluated in order to determine the fluctuations of the ethanol production capacity caused by the impact of the years. As a result 2425.44–4043.6 l ha-1 theorical ethanol capacities have been calculated, which means that sweet sorghums can be an adequate stock to produce bioethanol. -
SSR based characterization of peach (Prunus persica L.) and apricot (Prunus armeniaca L.) varieties cultivated in Hungary
17-24Views:306The SSR (Simple Sequence Repeat) markers allow the discrimination of the cultivars and determination its specific DNA fingerprints. The aim of this research was to evaluate fifteen apricot (Prunus armeniaca L.) and fifty-one peach (Prunus persica L.) genotypes cultivated in Hungary to obtain their DNA fingerprints in 6 SSR (Simple Sequence Repeats) loci by allele numbers and sizes.DNAs were extracted from leaves. PCR was carried out with CY-5 fluorescent labeled Prunus microsatellite markers and the products were separated on polyacrylamide gel with ALF (Automated Laser Flourometer)-Express II.According to our results, in the case of peach genotypes, all 6 SSRs were able to amplify alleles. UDP 96 005 was the most informative marker and UCDCH 17 was the least due to its monomorphic pattern. Regarding the apricot samples BPPCT 041 did not amplify any allele. In the case of P. armeniaca UDP 96 005 had the highest heterozygosity index as well and the highest number of alleles. The least informative marker was the UCDCH 17. Since the 6 SSR were not enough to discriminate the apricot and peach genotypes, it is suggested to use more SSR primers. -
Genetic progress in winter wheat quality and quantity parameters
71-75Views:173Wheat production is significant branch of Hungarian crop production (with about 1 million hectares of sowing area). Weather anomalies resulted by climate change have increased the importance of biological basis in wheat production. Yield quality and quantity parameters of three wheat genotypes sown on chernozem soil type after maize pre-crop were studied in a long-term field experiment. Yield amount of the studied genotypes varied between 2894 and 8074 kg ha-1 in 2017 and between 5795 and 9547 kg ha-1 in 2018 depending on the applied treatments. Based on our results it can be stated that in both studied crop years the highest yield increment was realized by the application of the nutrient supply level of N30+PK. As the result of the application of the optimum mineral fertilizer level – in contrast to the control – resulted in significant yield increment in both crop years. The results of the long-term field experiment prove that water utilization of the studied wheat varieties / hybrids was improved by the application of the optimal nutrient supply. Furthermore, the water utilization of the latest genotypes was more favorable by both the control and the optimum nutrient supply level treatments. Analyzing the quality parameters of winter wheat using the NIR method it has been stated that the quality results of the well-known genotype (GK Öthalom) were better than those of the new genotypes. A negative correlation between winter wheat quality and quantity parameters has also been confirmed. As the result of the mineral fertilizer application protein and gluten content of winter wheat increased to a significant extent.
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Investigating the above-ground biomass values of sweet potatoes (Ipomoea batatas)
35-39Views:156The role of sweet potato tubers in human nutrition is not new. The above-ground biomass of sweet potatoes is not used for nutritional purposes in most countries, but it has a high biological value. Therefore, the aim of the present study is to investigate the production of press fibre from above ground biomass by wet fractionation. Two sweet potato varieties (purple- and white-fleshed sweet potato) and two types of irrigation system were used: bubbling water flow system (BWS) and continuous water flow system (CWS). Glucan, xylan, arabinan were analysed by HPLC and elemental content was measured by ICP-OES. Our results show that the total carbohydrate content in the pressed fibre of the leaf blades (27.64–29.88 %w/w) is lower than in the stem with petiole (51.14–57.36 %w/w). No significant difference in glucan, xylan and arabinan content was observed in the leaf blade. In the stem with petiole, significant differences were observed for xylan and arabinan contents. For elemental content, generally higher values were measured in the leaf blade than in the stem with petiole. This information may be relevant for the selection of the appropriate variety and treatment, even for the production of functional food.
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Pest-fauna of grasslands and seed-grass varieties
56-59Views:131The present paper deals with various herbivorous species living in grassland ecosystems. The research focused not only on the complexity of pests in grasslands but also on individual pest species; their biology and the severity of damage, with special regard to seed feeders of some frequent herd’s grass species. The observed pests are showed in two ways, both taxonomically and according to their localization on the crop that is where they cause damage. An attempt was made to present the results being easily understandable for practitioners.
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Investigation of the milk composition of three Tsigai sheep varieties, and the detection of cazein fractions of sheep milk by Reversed-Phase HPLC
13-22Views:107The measurements started in 2005, the author used the milk of 3 types of Tsigai Sheep that are suitable for milking, they examined the milk composition, the possible differences between the milk types, the coagulation characteristics, cheese processing, yielding characteristics and the transfer of solid content into cheese. From the large number of measurements, the results of milk composition examinations are described.
During the research the author examined the milk coagulation property of three types of Tsigai Sheep QTS 25 is a substance investigating instrument, and its primary mission is the analysis of the substance profile. The advantage of this instrument is the representation of the survey graphs from which the part of the substance characteristics could be directly read off. By the help of the applied mathematical and statistical operations we tried to establish connection between the congealment-hardness and the specialty of the different types of Tsigai Sheep.
In 2006 the author began the identification and qualification of the major sheep milk proteins by RP- HPLC method. She used a scientific article written by Bordin et al. The method is able to separate and quantify the seven major proteins. However Bordin’s procedure was developed using bovine milk, the author adapted it to sheep milk. -
Application of GIS, precision agriculture and unplugging cultivation in plant breeding of Karcag
49-56Views:187In the last two decades, the prevailing ecological conditions and climate change have caused negative effects. Therefore, a paradigm shift is needed in the field growing of plants. The latest inventions, digital technologies, precision cultivation are not enough, the mentality of the farmers is more important. For this reason, not only big financial sacrifices, but adequate receptivity are needed on behalf of farmers. Adequate skills and continuous self-education are necessary. The yield of plant growing farms is determined by ecological conditions to a 40% extent, genetic background of the seed has a 30% share and the applied agricultural technology has a proportion of 30%. In different agroecological conditions, bred varieties of plants have bigger tolerance to unfavorable factors of the regions and significant yield stability. Farmers, who buy and sow seeds, can only contribute to the genetic potential of the seeds with cultivation technology. Plant breeding provides stable genetic background and good quality seeds. Breeding activity – choosing variety proposants, breeding them, selection work, classical breeding process for 8-10 years – must create new landraces, which can produce balance, high yield and have good quality parameters in extreme ecological conditions, yearly excursion and have higher tolerance to unfavorable factors of the region giving significant production stability for farmers. In Karcag GIS technology, precision cultivation elements and soil-friendly agrotechnical methods have been introduced which largely support the aims of breeding and can also provide optimal cultivation conditions in extreme years. Because of the specificity of breeding the main aim is not only to increase yield but to provide harmonic growing for bred materials, to decrease the number and the cost of cultivation and to be punctual. In this study, applied new methods and technologies are introduced.
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Analyses of a few physiological parameters of hybrid wheat in the case of different nitrogen supply levels
49-53Views:214The winter wheat is one of the most determinant crops because its role was always important in human’s life. To increase the average yield there are several possibilities, which are still not clear fields of agricultural plant production. Our main goal was to examine the responses of winter wheat genotypes to different amounts of nitrogen supplies. The sowing area of hybrid wheats are increasing, they may have different nutrient nitrogen utilization compared to varieties, and the question arose if it is possible to achieve same yield at lower nitrogen fertilizer application or not.
The present study analyzes the results of winter wheat (Triticum aestivum L.) from tillering growing stage. Under controlled conditions three different wheat hybrids were grown (Hywin, Hystar, Hybiza) with two different amounts of nitrogen supplies (optimal and the fourth part). The dry matter accumulation, relative chlorophyll content and nitrogen content were measured in order to draw conclusions from the different supplies of nitrogen for winter wheat genotypes and their physiological plasticity.
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Use of molecular marker methods in the classification of bamboo taxa: A review
51-59Views:254Bamboo plants are currently attractive to researchers because of their versatile uses. Understanding the bamboos’ genetic level is needed to develop new varieties. Taxonomic identification is the basis for plant development. Bamboos were identified as their taxonomical morphological characters which are dependent on environmental factors. Molecular Marker techniques can be used to perform accurate genotype identification, which can be used for genetic diversity analyses. The RFLP, RAPD, AFLP, SSR, ISSR, iPBS, SCARS, SCoT, SRAP marker systems have been shown to be able to efficiently determine the genetic diversity of bamboo species based on genotyping. This paper summarizes research that aims to analyze the genetic diversity of bamboo species on a molecular basis.
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The effect of different genotype cattle grazing on the nutrient content of saline grasslands vegetation
46-50Views:208Maintaining saline grasslands in good condition, preserving their yield and diversity is important not only for the purpose of nature conservation, but also for farming. Therefore, the primary purpose of our study is to analyse the effect of pasture use of the smaller weight extensive and the larger weight intensive beef cattle on the grassland vegetation and nutrient content. In this way, we can answer the question whether grazing for nature conservation can be achieved with more profitable, more economical and more intense varieties.
The tests were carried out in May 2016 and May 2017, in the Hortobágy National Park (Pap-ere and Zám puszta), where a total of 16 sample areas were analysed. These areas are grazed with extensive beef cattle (Hungarian grey) and mixed genotype of intensive cattle. The associations were selected along a moisture gradient, such as wet salt marsh (Bolboschoenetum maritimi) and drier saline meadow (Beckmannion eruciformis). All the vegetative material collected both years in May was analysed for the following parameters: dry matter, crude protein, crude fibre and life-sustaining net energy content. We compared the effects of medium grazing (0.46 livestock/ha) and abandonment on vegetation and nutrient content.
We examined the effect of (i) grazing, (ii) different grasslands (salt marsh, saline meadow) and (iii) grazing of different cattle breeds (Hungarian grey, intensive beef) on the nutrient content of the vegetation of grasslands Based on our results, it was found that grazing had an impact on crude protein and life-sustaining net energy content. The highest crude protein content (12.75 m/m%) was obtained in the year 2017 in the area where higher density had been grazed for two years. For the lifesustaining net energy, the highest value (5.05 MJ/kg d.m.) was also obtained in 2017 and the lowest in 2016. Furthermore, it was found that there was no significant difference between the effect of the two cattle breeds on the parameters examined. Significant effects were observed only in the case of life-sustaining net energy: in the area of intensive beef cattle we received a higher value (5.15 MJ/kg body weight) than in the area with extensive beef cattle (4.96 MJ/kg body weight).
Our results have also shown that cattle grazing is of the utmost importance for the maintenance of both wet and mesophilous habitats. Based on our three-year study, we can say that grazing by both extensive and intensive cattle breeds is suitable for the management of saline habitats.
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Dieback of apricot plantations caused by 'Ca. Phytoplasma prunorum' in Borsod-Abaúj-Zemplén county (Northern-Hungary)
34-41Views:132Plant diseases caused by phytoplasmas have increasing importance in all over the world for fruit growers. Lately, phytoplasma diseases occur on many fruit varieties and responsible for serious losses both in quality and quantity of fruit production. In the long-run these diseases cause destruction of fruit trees. The apricot phytoplasma disease (Ca. Phytoplasma prunorum) was first reported in Europe in 1924 from France. In 1992 the disease has also been identified in Hungary. On the base of growers' signals serious damages of "Candidatus Phytoplasma prunorum" Seemüller and Schneider, 2004 (formerly: European stone fruit yellows phytoplasma) could be observed in different stone fruit plantations in the famous apricot-growing area nearby Gönc town, Northern-Hungary. Field examinations have been begun in 2009 in several stone fruit plantations in Borsod-Abaúj-Zemplén County mainly in Gönc region which is one of the most important apricot growing regions in Hungary, named “Gönc Apricot Growing Area”. Our goals were to diagnose the occurrence of Ca. Phytoplasma prunorum on stone fruits (especially on apricot) in the North-Hungarian growing areas by visual diagnostics and confirm data by laboratory PCR-based examinations. All the 28 collected samples were tested in laboratory trials and at 13 samples from apricot, peach, sour cherry and wild plum were confirmed the presence of phytoplasma (ESFY). On the base of observations it seems evident that the notable losses caused by "Ca. Phytoplasma prunorum" is a new plant health problem to manage for fruit growers, especially apricot producers in Hungary.
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Changes in the Diaporthe helianthi Infection of Sunflower Hybrids Between 1998 and 2002
214-233Views:77Plant protection, and especially the efficiency of protection against mycosis, is a very important production technological element concerning sunflower. The efficiency of production can be increased on the basis of a thorough pathological survey and its results carried out in a wide variety of hybrids, as the features of hybrids, the reactions of genetic bases to pathogens can be found out under domestic conditions.
The tests were carried out at variety-test lots of OMMI for hybrids used for food or oil and other sunflower varieties admitted by the state at the Experimental Site DTTI Látókép, Centre of Agricultural Sciences, University of Debrecen between 1998 and 2002. The number of the tested hybrids was 49 in 1998, 45 in 1999, 49 in 2000, 55 in 2001, and 44 in 2002.
Due to the infection source of high amounts remained from 1997 and the favourable weather conditions for the pathogens, an infection of high degree was experienced in the tested stands. The lowest infection index in the hybrids with a very short vegetative period was experienced with Beni hybrids. Comparing to the average of the hybridgroup with short vegetative period, a low infection index characterised the Baleno, Trident, IBH-166, Hysun 321, Resia, Alexandra, Cergold and Pixel hybrids. In 1999, among hybrids with medium vegetative period, Zoltán, Zsuzsa and Util hybrids could be highlighted because of their low infection index. In 1998, among confentionary hybrids, Marica-2 hybrid had the most favourable index values (2,55).
During our trials, the experienced infection dynamics were compared in the event of hybrids with higher and lower susceptibility under different sowing technological elements. The results call for the fact that when a hybrid with higher susceptibility is produced, production technological elements, such as the time of sowing, influence considerably the damage caused by the pathogen, therefore it must not be ignored when its determination takes place. In the event of hybrids with lower susceptibility the change of the tested production technological elements within the biological optimum does not lead to the increased risk of the damage caused by Diaporthe helianthi. -
Plant production possibilities on a heavy metal contaminated soil with the purpose of biorefinery
215-222Views:126Significant part of not cultivated area of Hungary is not suitable for agricultural utilization because of industrial
pollution. Technologies of biorefinery make reutilization of contaminated areas possible. Biomass of plants
produced on polluted soils can be raw material of valuable products. Applicability of biorefinery was tested on a
heavy metal polluted soil, where the contamination originated from previous mining activity. Complete biomass
utilization was aimed to obtain cosmetic ingredients, pharmaceutical agents, and precursors. During our research
work 88 plant species and varieties were produced and tested for potential utilizable components. Levels of
possible contaminants in these plants were monitored, and amounts of carbohydrates, protein, organic acid and
cellulose were determined as well. Different plant extracts were tested as potential sources of biologically effective
components or as raw materials for lactic acid fermentation. Our results show that biorefinery is a real possibility
for utilization of polluted areas. Numerous plants could be cultivated on contaminated areas without increased
levels of contaminants in their tissues, thus they can be sources of valuable compounds. -
Sour cherry as a functional food
41-47Views:190The antioxidant capacity of ’Debreceni bőtermő’, ’Újfehértói fürtös’ and ’Érdi bőtermő’ cultivars were determined by FRAP (Ferric Reducing Ability of Plasma), DPPH (1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity) and photochemiluminescence method. In sour cherry, the most antioxidant effects of natural bioactive compounds are anthocyanins. Our results show that the photochemiluminescence method out of applied assays is ratheris suitable to determine the antioxidant capacity of red soft fruits and tart cherries. The correlation is good between the determined anthocyanin concent by this technic and pH-differential spectrophotometry. However, both FRAP and DPPH assays are inaccurate.
The anthocyanin composition of ’Debreceni bőtermő’, ’Újfehértói fürtös’ and ’Érdi bőtermő’ ’Csengődi csokros’ sour cherry varieties were analised. There are big differences between the accumulation of anthocyanan compounds of cultivars. ’Csengődi csokros’ produce melatonin in large quantity. On the evidence of the results, we can say that the hungarian sour cherry cultivars are suitable for functional food development.
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Effect of cropping technologies on the yield of dry bean variety ’Diana’
37-41Views:139An experiment on three dry bean varieties (Start, Hópehely, Diana), using different sowing-times, fertilizers and plant densities was performed on sandy soil in the University of Debrecen, Centre for Agricultural Sciences, Research Institute of Nyíregyháza in 2015. The aim of the experiment was to study which treatment gives the highest yield, and whether the different treatments result in significant differences in the yields. In this paper the dry bean yield at ‘Diana’ variety is analyzed.
The treatments were done with three fertilizer doses and three plant densities at different sowing-times (April 24; May 8; May 18). As a result of the high temperature and the drought during the growth season, the yields we harvested were in low, which shows the ecological sensitivity of the plant we examined.
We concluded that the poorest yield was harvested at the third sowing-time. There was no significant difference in the yields at the first and second sowing- time. Examining all the three fertilizer treatments we applied at the experiment, we achieved the highest yields in the control plots. It might be due to the weak efficiency of nitrogen fertilizer under the extremely dry conditions. The highest yield was harvested at the control treatments during the second sowing-time. Regarding the effect of the plant densities, the highest crop yield was achieved at the treatment using 400 000 germs ha-1, followed by 300 000 germs ha-1 and 200 000 germs ha-1.
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The efficacy of combining paraffin oil with conventional fungicide treatments against grape powdery mildew in Eger
173-180Views:174We aimed to test the combination of paraffin oil (PFO) with regular fungicide treatment to assess its efficacy against grape powdery mildew (GPM) in a small spraying experiment on two Vitis vinifera L. cultivars (Chardonnay and Kékfrankos) with different susceptibility to Erysiphe necator. The visual symptoms of GPM on leaves and clusters were examined at three phenological states. The harvest yield was characterized by two methods, data were analyzed with one-way ANOVA and Tukey post-hoc test. Regular fungicide treatment (CT) and its combinations with PFO showed better results in both varieties to repress GPM in 2015 relative to sole PFO treatments. Mean values of combined treatments were often lower than CT but did not differ significantly from each other. The same was observed in 2016, despite the higher pressure of GPM, and missed the third survey. No significant differences were detected between treatments in yield. In contrast, the mean cluster weight of CT and combined treatments resulted in (insignificantly) higher values in each variety and year. In summary, the sole PFO showed some disease control capability as reported earlier, but this effect was greatly affected by the given vintage. Combining PFO with CT resulted in increased protection against GPM relative to the solely applied fungicides. However, this effect was not significant in all cases. It also depended on the vintage and cultivar characteristics. The beneficial impact of paraffin oil as an additive to CT may be due to the induction of plant stress responses and/or its ability to support the adherence and absorption of the combined agents.
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Deproteinized plant juice as part of circular economy: A short review and brief experimental data
23-26Views:1536As the population of the Earth is constantly growing it generates an unmet demand for protein, which is an urgent problem. The protein extraction process is a potential solution, which offers high-quality plant protein suitable for animal and human nutrition at a favorable price. The process used within our project produces green juice from the green alfalfa biomass through pressing. After the coagulation of protein from this green juice, the by-product is called DPJ (Deproteinized Plant Juices) or brown juice. Our preliminary results match the international literature, namely that brown juice take up as much as 50% of the fresh biomass in weight. To utilize this by-product is a crucial part of the process to make it environmental-friendly and financially viable as well. The examined brown juice samples came from a small-scale experiment of alfalfa varieties carried out in the experimental farm at the University of Debrecen. According to our preliminary results, brown juice has high macro- and micronutrient values, furthermore, it has a potentially high amount of antioxidant compounds. The study highlights that brown juice is suitable as an ingredient in microbiological media, in plant nutrition as a supplementary solution, for feedstock and for preparing human food supplements or functional foods. The potential utilization of all biorefinery products makes it a very appropriate technology for today’s challenges.
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Results of fast neutron induced mutations in M1 and M2 generations of sorghum (Sorghum bicolour)
49-54Views:98Due to climatic barriers, the genetic variability of Sorghum varieties decreases away from the gene centre, therefore hybrids cultivated in Hungary are restricted to a relatively limited genetic basis. This limited genetic basis results in a lower rate of genetic improvement, so the genetic basis of cytoplasm factors are to be extended. We enhanced the diversity of primary materials by fast neutron treatment. As a result of this treatment we detected sterile, deformed specimens with double panicles in the stock treated with a dose of 12.5 Gy in the M1 generation. Agronomically useful
specimens were found in the stock treated with 10 Gy and 7.5 Gy doses, where the leaf area index (LAI) and Harvest- index gave higher values than those of the control group. In the stock treated with 5 Gy dose, variations were less significant. In the reseeded M2 generation we detected albino specimens with extreme chlorophyll defects in the 10 Gy Zádor stock.