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The effect of crop protection and agrotechnical factors on sunflower in the Hajdúság region
39-46Views:88Extreme weather conditions are becoming more and more frequent in the crop years, thus increase the risk of sunflower production.
The objective of researches into plant production is to minimize these effects as much as possible. In this sense, the optimization of
agrotechnological factors is of high importance. Within these factors, the appropriate crop technology (sowing time, crop density)
and optimized, rational crop protection technologies are important, especially in the highly sensitive sunflower cultures. The effect of
sowing time, crop density, and fungicide treatments on the yield of sunflower hybrids was analysed in different crop years in 2008
and 2009. In each case, the infection was highest with the early sowing time and at the highest crop density level (65000 ha-1). When
one fungicide treatment was applied, the rate of infection decreased compared to the control treatment. The further decrease of the
infection rate was less after the second fungicide treatment.
In the humid year of 2008 the crop yield was the highest at 45000 ha-1 crop density level in the control treatment and at 55000 crop
ha-1 crop density level when fungicides were applied. In the draughty year of 2009 the maximum yield was gained at 55000 ha-1 crop
density level in the control treatment and at 65000 crop ha-1 when fungicides were applied. In 2008 and 2009 as regards the crop
yield, the difference between the optimal and minimal crop density levels was higher in the fungicide treatments than in the control
treatment (in 2008: control: 517 kg ha-1; one application of fungicides: 865 kg ha-1; two applications of fungicides: 842 kg ha-1), (in
2009: control: 577 kg ha-1; one application of fungicides: 761 kg ha-1; two applications of fungicides: 905 kg ha-1).
In each and every case, the first treatment with fungicides was more effective than the second. In 2008, the highest yield was
obtained with the third, late sowing time in each fungicide treatment. The differences between the crop yields with different sowing
times was less than in 2009, when the results of the second treatment exceeded those of the first and third treatment in each case. -
Nitrogen Supplying Capacity of Brown Forest Soil under Different Cropping Practices and 0.01 M CaCl2 Soluble Organic Nitrogen
17-23Views:94The best known and most remarkable example of continuous production in Hungary is the Westsik’s crop rotation experiment, which was established in 1929, and is still in use to study the effects of organic manure treatment, to develop models, and predict the likely effects of different cropping systems on soil properties and crop yields. In this respect, Westsik’s crop rotation experiment provides data of immediate value to farmers concerning the applications of green, straw and farmyard manure, as well as data sets for scientific research.
Although commonly ignored, the release of nitrogen by root and green manure crops has a significant impact on soil organic matter turnover. The design of sustainable nitrogen management strategies requires a better understanding of the processes influencing nitrogen supplying capacity, as the effects of soil organic matter on soil productivity and crop yield are still very uncertain and require further research. In the treatments of Westsik’s crop rotation experiment, nutrients removed from soil through plant growth and harvesting are replaced either by fertilisers and/or organic manure. Data can be used to study the nitrogen supplying capacity of soil under different cropping systems and its effect on the 0.01 M CaCl2 soluble organic nitrogen content of soil.
The aim of this paper is to present data on the nitrogen supplying capacity of brown forest soil from Westsik’s crop rotation experiment and to study its correlation with hundredth molar calcium-chloride soluble organic nitrogen. The main objective is to determine the effects of root and green manure crops on the nitrogen supplying capacity of soil under different cropping systems. The nitrogen supplying capacity was calculated as a difference of plant uptake, organic manure and fertiliser supply.
The 0.01 M CaCl2 soluble organic nitrogen test has proved reliable for determining the nitrogen supplying capacity of soils. Brown forest soils are low in organic matter and in the F-1 fallow-rye-potato rotation, the nitrogen supplying capacity was 15.6 kg/ha/year. 0.01 M CaCl2 soluble organic nitrogen content was as low as 1.73 mg/kg soil. Roots and green manure increased the nitrogen supplying capacity of soil by more than 100%. This increase is caused by lupine, a legumes crop, which is very well adapted to the acidic soil conditions of the Nyírség region, and cultivated as a green or root manure crop to increase soil fertility. -
Adopt the tobacco plastid transformation technics in Hungary
73-76Views:80Under the GENOMNANOTECH Debrecen Regionális Egyetemi Tudásközpont (GND RET) research program in summer 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture experiments were launched to transform tobacco plant by plastid transformation technics.
Our aim was to adopt first in Hungary the tobacco plastid transformation technics, which were used in Waksman Istitute Rutgers, The state University of New Jersey (USA) leading with Prof Dr. Pál Maliga. Scientists won scholarships learn and use this technics in the University of Debrecen. -
Effect of G2548A polymorphism in the leptin gene on the BMI level in human population
5-10Views:140The polymorphism in leptin (LEP 2548A) seems to influence obesity among others genes. The aim of this study is to investigate the effect of the G2548A polymorphism on body mass index. We included 79 people from Slovakia with some genetic relatedness and used barrels kit to isolate the genomic DNA from an adenoblast swab- from the salivary. PCR products were amplified by pursued polymorphisms and G2548A, we restriction-analyzed them and then we identified the specific fragments describing the presence of chosen SNP polymorphism by the agarose electrophoresis, to analyze SNP polymorphism by PCR-RFLP method.
The LEP gene had increased frequency of G allele (0.5506). The most common genotype occurring in the gene LEP was heterozygous genotype (AG) and the least frequent genotype in LEP was AA (0.1899). Taking the age into account the BMI is higher if the G allele occurs in the LEP gene. Moreover, if the G allele genotype was situated in dominant form, then the highest average BMI was present.
According to the results we can assume that the AA genotype (LEP) has a protective effect on the prevalence of obesity compared to the other genotypes.
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Leaf and root evaluation of bioactive compounds of different beetroot varieties
135-139Views:202The importance of beetroot lies in the numerous health benefits, attributed to its wide-ranging array of minerals, vitamins and bioactive compounds which play a key role in the human diet. Generally, beetroot is consumed for its root, however, its leaves also contain a good amount of bioactive compounds. The experiment was carried out with four beetroot varieties in order to compare the amount of bioactive compounds in the root and the leaf. The analyzed data were total dry matter content, total polyphenol, flavonoid and vitamin C.Results have shown that roots contain the highest amount of bioactive compounds. Nevertheless, leaves can be also considered a significant source. Therefore, consumption of the young leaves is highly recommended due to their availability after thinning or at maturity stage (60 days after the germination). Of the examined varieties, ‘Libero’ demonstrated the highest total polyphenol, flavonoid and vitamin C values, although it was found to be statistically insignificant. -
Nutrient Uptake of Miscanthus in vitro Cultures
23-24Views:78The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.
Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that, after 35 days, nitrogen and phosphate were nearly completely taken up. From that time, shoot growth was not observed.
After shoot propagation, the plants were transfered into a nutrient solution for root formation (modified Murashige and Skoog, 1962), basic medium supplemented with 0,5 mg l-1 Indole- 3-Butyric acid, and could be potted in soil after about 14 days. -
Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:294The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.
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Impact of weather on the spring crops yield in Croatia with emphasis on climatic change and the 2014 growing season
41-46Views:162Main field crops in Croatia are maize, soybean, sunflower and sugar beet. By these crops are covered (status 2014) close to 50% (385 234 ha) of utilized arable land. Global warming, have often adverse influence on field crop yields. Aim of this study was testing precipitation and temperature regimes on spring crops yield in Croatia in 15-year period (1999–2013) and elaboration of the 2014 growing season with aspect of climatic change.
Four growing seasons (2000, 2003, 2007 and 2012) were less favorable for maize because annual yield was bellow 5 t ha-1 (average 4.38 t ha-1), while in four more favorable years (2005, 2008, 2009 and 2010) annual yield was above 6.8 t ha-1 (average 7.32 t ha-1). Average precipitation and temperature for the April-September period in Osijek were 226 mm and 496 mm, 19.6 oC and 18.6 oC, for less and more favorable years, respectively. Yields of soybeans and sugar beet have mainly similar trend as maize yields in function of weather conditions, while sunflower is more susceptible to extremely moist growing seasons (for example, 2001 and 2005: 650 mm and 697 mm precipitation and very low yields in level 1.7 and 1.6 t ha-1, respectively). On the other side, under drought conditions of 2003, 2007 and 2012, yields of sunflower were above average in range from 2.5 to 2.7 t ha-1), while at same period yields of maize, soybean and sugar beet were drastically reduced.
Average precipitation in the April-September period of 2014 for eight selected sites of Croatia was 756 mm or for 68% higher in comparison with the long-term average 1961–1990 with variation among the sites from 520 mm in Osijek to 910 mm in Varazdin. On the other side, average air-temperature in 2014 was 17.8 oC or for 0.7 oC higher with variations among the sites from 17.2 oC in Daruvar and Varazdin to 18.2 oC in Osijek and 18.3 oC in Gradiste. Under these favorable weather conditions, annual yields of maize (8.1 t ha-1), soybeans (2.8 t ha-1) , sunflower (2.9 t ha-1) and sugar beet 63.6 t ha-1) were considerable higher than usual.
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Evaluation of mineral element content of beetroot during the different stages of the growing season
459-469Views:264In modern nutrition, bioactive materials of different vegetables are especially important to be researched. The experiment was carried out on March 30, 2016 (sowing date). Mineral element content (B, Ca, Cu, Fe, K, Mg, Na, P, S, Zn) was evaluated on the 60th, 85th and the 105th day of the vegetation period. The data are presented as the mean of five varieties which can give the real state of these parameters of beetroot grown on lowland chernozem soil.
In the young beetroot leaves (60 days) the mineral element content was higher than in the older ones (85 days). The calcium and magnesium content of the leaves was much more favourable (10 times higher) than in the root. The potassium content of leaves has reached the amount of 5000 mg kg-1, but at the same time more than 3000 mg kg-1 was detected in the improved root. This value is favourable for the potassium supply of the human organism.
The sulphur content (1300 mg kg-1) of the leaves was the highest on the 85th day of vegetation period. Similar tendency was detected for boron content (2.45 mg kg-1), while for iron content it was higher (28.23 mg kg-1) in the younger leaves (60 days).
Finally, it can be concluded that the increased element content of beetroot leaves will be a favourable source of mineral element supply of the human organism.
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Nutrient deficiency and effects of various nutrition technologies on crop health
109-113Views:241The impacts of climate change on crop production are increasingly noticeable. Extreme weather conditions – such as devastating droughts, which occur more often – have serious effects on crop conditions, thus damaging their defence ability against pathogens and pests. Therefore, in order to achieve high-quality and high yielding crops, it is urgent to elaborate new technologies that improve general condition of crops and prevent development of nutrient diseases. Those crops which suffer from the lack of certain nutrients are more sensitive and their tolerance against diseases are decreased. Nitrogen – as the most influencing macronutrient in yield – is also essential in maintaining crop health. Nevertheless, due to the complicated processes in soil (such as leaching, denitrification), the utilization of nitrogen is not nearly complete, therefore nitrogen stabilizers may be needed to maximize this factor. The use of these stabilizers can be promising where plants with high nitrogen content are grown, although further experiments are needed in which impacts of nitrogen stabilizers on crop protection aspects are examined as well, since there is a close correlation between exaggerated nitrogen fertilizing and sensitivity to pests. During my research I am going to examine the combined effect of foliar fertilizer and nitrogen stabilizer on crop health. Furthermore, my goal is to find clear correlation between pathogens and the different technological variants of nutrition.
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Basic Research for the Development of Fertiliser Spreaders
52-57Views:87The knowledge of the physical characteristics of fertiliser particles is essential for the constructors and operators of fertiliser distributors. Among physical characteristics, the most important are the frictional and aerodynamic properties for the description of particle movement. Adjustable angled slopes, shearing boxes and various rotating disks are used to identify frictional properties. We have developed a high precision shearing box with digital force measuring cells and a distance signaller (incremental transducer) that we use for slide tests efficiently. We measured the frictional characteristics of 6 different fertilisers: the inner coefficient of friction and the coefficient of friction on ten test surfaces most commonly used in machinery, and we specified the relationship between displacement, loading and the coefficient of friction. We can conclude that the material of the frictional surface significantly influences the force of friction.
However, our experience tells us that the shearing box is not suitable for the measurement of the inner friction, since the examined particles slide on the metal surface of the shearing box in a growing extent in the course of displacement, so it does not measure the real inner friction. Therefore, in our experiment we have developed rotating shearing equipment with a constant shearing surface to identify the inner friction. We tested the equipment with fertilisers and we identified the inner frictional characteristics of 6 different fertilisers. With the developed rotating shearing apparatus we could measure the real inner friction of the particles.
To identify the aerodynamic characteristics of granules, wind tunnels and free-fall tests are used. An elutriator have been developed for our investigation. We have used fertilisers for testing the measuring equipment and we have identified the aerodynamic characteristics of 6 different fertilisers. -
Texture analysis as a method for grape berry characterization
Views:217The marketability of table grapes is mainly characterized by berry size, color, taste and texture. Mechanical measurements of table grape berries could provide objective information on the textural qualities of grape berries. In addition, this method might be suitable to study the effects of phytotechnical methods (such as girdling) on table grape quality. The aim of this preliminary work was to demonstrate how instrumental testing could be used to examine the effects of girdling on berry grape texture and define the textural characteristics of table grape berries. Cane girdling was carried out at veraison in two table grape varieties. Texture analysis was performed several times during the maturity. Besides this examination another five varieties were analyzed to assess their berry mechanical properties. Double compression test was used to determine berry hardness and its derived parameters. Puncture test was applied to evaluate skin hardness, skin elasticity and skin break energy. Skin thickness was also investigated. Berry hardness, skin hardness and skin thickness of the girdled grapevines were significantly affected by this technique. Most of the textural parameters showed differences among the seven cultivars.
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The olive (Olea europaea) and the almond (Prunus amygdalus) related phytonutrients, and the associated health-promoting biological effects, a review
11-24Views:224With the increasing attention to the health promoting activities of the bioactive compounds from some plants, many researchers are focusing on the biological potential and mechanisms of certain cultivated plant species. In this review, we survey the olive and almond based extracts specific phytoconstituents and their associated health promoting effects that have been evaluated in experimental and clinical studies.
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The recent state of cryopreservation techniques for ex-situ gene conservation and breeding purposes in small ruminants: A review
81-87Views:290The viewpoint of the recent cryopreservation techniques (CT) suggests the use of a reduced volume of cryopreservation solution, high concentration of cryoprotectants and ultra-rapid cooling and warming rates help to reduce cryo-injury and maximize the viability of the preserved animal genetic resources (AnGR). The CT had now become widely accepted as one of the best methods of choice for the ex-situ conservation of AnGR due to its high success rate recorded and no-invasive nature as compared to the conventional slow rate freezing (CSRF). Rapid advances and wide acceptability of the use of assisted reproductive technologies (ART’s) particularly artificial insemination (AI) in animal breeding had resulted in a greater loss of a large number of good quality genes in virtually almost all the native breeds of animals across the globe. Small ruminant (SR) animals are not an exception in such present predicaments situation of erosion and dilution of the valuable AnGR among the native breeds. As a result of this, 148 and 16 breeds of sheep and goats respectively have already become extinct in Europe and the Caucasus. In view of the aforementioned situation, the present review aimed at exploring some of the current states of development, roles played and potentials of CT in the conservation of SR genes and genome for the immediate and future breeding purposes for sustainable development. It basically covers; animal genetic resource, the need to conserve AnGR, tools for ex situ in vitro conservation of AnGR and recent developments in breeding and cryopreservation of SR AnGR.
Cryopreservation is playing a pivotal role in ex-situ gene conservation of AnGR. Decline in genetic diversity among SR breed population was high in Europe and the Caucasus. There is therefore, need for improvent on current stringent measures on conservation of AnGR in this region of the world.
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Findings on the cultivation of potatoes in organic farming
113-116Views:91This paper explores the effectiveness of organic and plastic mulching for potato production in the Czech Republic. The mulching with chopped grass (GM) and black textile mulch (BTM) were compared to non-mulching control variant (C) with mechanical cultivation. Especially in plots with BTM were first formed ridges and covered by the black polypropylene non-woven textile and then they were planting. During vegetation the infestation of Colorado potato beetle (CPB), weeds biomass, course of soil temperature and soil water potential were assessed. The results showed that surface of GM had a positive effect on soil temperature reduction, soil water potential depression. This study also indicated a positive effect of GM on the larvae of CPB diminution, on the other hand higher incidence of larvae and higher defoliation was observed in BTM. GM had a significant effect on the yield of potatoes. The yield of ware potatoes was higher by 27 % higher on plots with GM and by 16 % lower on plots wit BTM in comparison with C. NeemAzal T/S decreased statistically significantly % of defoliation and increased yield of ware potatoes by 35 % in comparison with control.
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Researches regarding the influence of the some technological elements on water use efficiency in maize from Crisurilor Plain
5-9Views:139Plain and the influence of the hybrid, plant density, crop rotation, nutrient supply, weeds and irrigation on water use efficiency were studied.
Choosing of the hybrid with the best water use efficiency is very important because a hybrid from 500–600 FAO group (Fundulea 376) in unirrigated conditions and a hybrid from FAO group over 600 (Fundulea 365) obtained the biggest water use efficiency; the hybrid Fundulea 365 obtained the highest irrigation water use efficiency, 20.1 kg yield gain 1 mm-1 irrigation water.
One of the most known hybrid in the area is Turda super and the highest water use efficiency was obtained using the plant density of 55 000 plants/ha in unirrigated variant and 70 000 plants/ha in irrigated variant. The highest irrigation water use efficiency, 20.7 kg yield gain 1 mm-1, was obtained at 70 000 plants ha-1.
In maize monoculture was obtained the lowest values of the water use efficiency in unirrigated and irrigated variant: in the wheat-maize crop rotation the values were higher than in maize monoculture and in the wheat-maize-soybean were registered the highest values. The same situation was registered regarding the irrigation water use efficiency.
Farm manure (30 t ha-1) and especially manure (30 t ha-1) +chemical fertilizers (N90P45) determined a higher values of the water use efficiency in comparison with the control. In the variant with organic + mineral fertilization was registered the higher value (19.4 kg yield gain mm-1) of the irrigation water use efficiency.
Water use efficiency was much lower in the variant with weeds in comparison with the variant without the weeds; the differences were of 69% in unirrigated variant and of 64% in irrigated variant, very significant statistically. Irrigation water use efficiency from variant with weeds was lower than the value registered in the variant without weeds; the difference (68%) was very significant statistically.
In average in period 1976–2012, the irrigation determined the increasing in water use efficiency with 22%, 19.4 kg mm-1 vs. 15.8 kg mm-1, but not in all the years caused the irrigation increasing in water use efficiency in comparison with unirrigated maize.
The results research emphasized the need of the optimization for technology elements studied and a better water use efficiency will be obtained.
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Impact of the integration of lupine (Lupinus albus) into crop rotation on the extent of soil compaction in the Westsik longterm field trial
529-537Views:96In order to reduce or eliminate soil compaction, rational crop rotation and appropriate sequence of crops have an increasingly important role in addition to mechanical and tillage solutions. In this respect, introduction of greening in recent years has been a major step, which focuses on aspects of environmentally conscious, soil conserving farming and the improvement of biodiversity. The cornerstone of this strategy is the cultivation of crops that have a beneficial effect on soil properties, such as the use of nitrogen-fixing plants and green manure plants in the cultivation system that have a beneficial effect on soil structure. In our examinations, penetrometer measurements were carried out in the second longest crop rotation-based field experiment in Europe in order to quantify the effects of green crops and crop rotation strategies on soil resistance. Our aim was to evaluate and compare the impact of lupine (Lupinus albus) on the penetration resistance of soil on sour sandy soils. At the time of the penetration resistance measurement, different crop rotations had a significant effect on the development of the parameter in the examined soil layer. The most favourable penetration resistance values were found in the crop rotation, which included lupine as a green manure. The favourable effect is dominant below the cultivated layer (0–40 cm), which is statistically verified. The values of penetration resistance of the cultivated soil layer of lupine sown as primary green manure did not differ significantly from the values measured in the case of the fallowing-based crop rotation. Therefore, the use of lupine green manure instead of fallowing could be worth considering by practical application due to its favourable effects on soil penetration resistance. The use of lupine green manure after the production of rye cultivation resulted in penetration parameters similar to fallowing, irrespective of the green crop and the applied amount of nitrogen fertilizer, which justifies the cultivation of the crop as green manure. In the case of potato cultivating, recorded compaction within the cultivated layer is an obvious consequence of mechanical compaction during harvest; therefore, machinery operations are decisive for the development of penetration resistance values of the cultivated layer. In addition to the beneficial effect of lupine as a green manure crop on soil condition, its nitrogen-fixing ability is also important; it stresses the utilisation of the crop of sour sandy soils for the sake of proper soil management.
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Correlation between the weather in 2017 and the productivity of maize
89-93Views:160In our research we examined the effect of the hybrid, the nutrient supply, the number of plants and the abiotic factors (temperature, amount of precipitation) on the yield, crop quality and yield stability of maize. We devoted special attention to the natural nutrient utilization ability and fertilizer reaction of maize. The experiment took place in Hajdúszoboszló on chernozem soil, on a nearly 8 ha field. The size of one plot was 206 m2; therefore, this experiment was half-industrial. We tested six hybrids with different genetic characteristics and growing seasons. I analysed the correlation between the nutrient supply and the yield of maize hybrids with control treatment (treatment without fertilization) and with N 80, P2O5 60, K2O 70 kg ha-1 and N 160, P2O5 120, K2O 140 kg ha-1 fertilizer treatments. The yield increasing effect of the fertilizer also depended on the number of plants per hectare to a great extent. The number of plants of the six tested hybrids was 60, 70, and 80 thousand plants ha-1.In Hajdúszoboszló in 2017, up to October, 445.8 mm of rain fell, which is in line with the average values of 30 years, and is only 45.7 mm less than those. In 2017, the effect of increasing the plant number was slighter. Averaged over the observed fertilizer treatments and hybrids, the yield was 9.10 t ha-1 with 60 thousand plants ha-1, 9.11 t ha-1 with 70 thousand plants ha-1 and 9.12 t ha-1 with 80 thousand plants ha-1. Without fertilization, in most cases, increasing the plant number from 60 thousand plants ha-1 to 70-80 thousand plants ha-1 does not increased the yield but decreased it. With N80+PK treatment the yield changed between 8.90 and 11.27 t ha-1. The effect of increasing the plant number was just slightly observable and did not show a clear tendency. The effect of changing the plant number, even with the highest dosage of fertilizers, could not be detected adequately. In contrast with the plant number, the effect of the different fertilizer treatments was expressly traceable. Compared to the control treatment (treatment without fertilization), with N80+PK fertilizer dosage with 60 thousand plants ha-1 the yield increased by 3.36–4.99 t ha-1. The smallest demonstrable proof, i.e. the LSD5% was 0.22 t ha-1, which means that fertilization, in each case, significantly increased the yield. When analysing the effect of fertilization in the average of the hybrids and the different plant numbers, a yield of 5.61 t ha-1 could be detected, which value was 10.12 t ha-1 with N80+PK treatment and 11.61 t ha-1 with N160+PK treatment. Thus, it can be calculated that compared to the treatment without fertilization, the N80+PK treatment increased the yield by 4.51 t ha-1, while compared to the N80+PK treatment, the N160+PK treatment increased the yield by 1.49 t ha-1. In addition to agrotechnical factors, in maize production, the impact of the crop year is specifically of high importance.The average yield of hybrids (in the average of the different fertilizer treatments) was 6.81 t ha-1 in 2015, 11.86 t ha-1 in 2016 and 9.11 t ha-1 in 2017. When comparing the yield results against the precipitation data, it is clearly visible that the amount of rain fell in the January– October period is directly proportional to the average yield of maize. The effect of the crop year can be defined in a 5.05 t ha-1 difference in the yield. -
Comparison of sample preparation methods for the identification of Staphylococcus Aureus by MALDI-FOF MS
9-14Views:281Coagulase-positive staphylococci include 3 species, Staphylococcus aureus, S. hyicus and S. intermedius. Of these three species, S. aureus is the most well-known human pathogen. S. aureus is part of the human and animal normal microbiota, however, it is capable of producing several staphylococcal enterotoxins (SEs) that cause intoxication symptoms of varying intensity in humans after consuming contaminated food. Selective media which are used for the determination of coagulase-positive staphylococci from foods are not able to identify isolates at a species. With the MALDI-TOF MS technique, we can identify S. aureus cheaper and faster than by using molecular methods. This paper describes the results of the study of the presence of coagulase-positive staphylococci and S. aureus in many food products, and the application of three sample preparation methods: direct sample preparation, formic acid suspension and ethanol extraction.
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Influence of temperature and variety on seed germination of soybean (Glycine max L. Merr) at different germination times
5-12Views:108Seed germination is an important stage in crop development that affects plant performance, crop yield, and quality. Many factors influence seed germination, and one of the most important factors is temperature. The closer the temperature is to the optimum, the faster germination will occur. Temperature affects seed germination in various plants and varies depending on the variety. Therefore, the effect of temperature on seed germination is necessary to investigate, also for soybean varieties. Soybean is one of the world's most valuable oil-seed crops. Generally, proper seedling establishment and germination of soybean seeds are crucial processes in the survival and growth cycle of the crop. Thus, a study was done to investigate the influence of temperature and variety on soybean seed germination at different times after sowing. The experiment was carried out at the Institute of Agronomy, Hungarian University of Agriculture and Life Sciences (MATE), Crop Production Laboratory. Seeds of two soybean varieties were subjected to three different temperatures: 15, 25, and 35 °C, and two soybean varieties, Martina and Johanna were tested. There were four germination times based on the days after sowing: Days 3, 5, 7, and 9. This experiment was set up with a completely randomized block design and four replications. According to the findings of this study, the Martina variety showed a better germination rate as well as higher vigor and viability than the Johanna variety in the first 12 days after sowing. At temperatures of 15 °C and 25 °C, both varieties achieved comparable percentages of viability, but they were more vigorous at 25 °C due to better total seedling length. Thus, the information gained from this study will indirectly determine and confirm the proper temperature for the initial growth of the Martina and Johanna varieties.
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Development of the reproductive tract of immature gilts
165-166Views:63Mechanisms of regulation of litter size in pigs are complex and depend on many factors, including genetic regulation and also physiological and anatomical development of the reproductive tract in gilts. Improvement of growth rate in present breed pigs raises the question as to whether, with the development of the reproductive tract, sexual maturity would also be attained.
The aim of the study was to assess the morphometric traits of reproductive tracts taken from gilts slaughtered at 100 kg body weight, i. e., just when they may become actively sexual mature.
This study was concluded on 80 prepubertal gilts of the Polish Landrace (PL) breed tested at the Pig Program Testing Station. The animals were kept in individual pens with control feeding and standard management. They were slaughtered after attaining 100 kg body weight. Immediately after slaughter, the reproductive tract was removed and carefully assessed. The morphometric estimation of the reproductive tract involved the
measurement of uterus weight with ligament, vagina-cervix length, uterine horns and oviducts length, ovaries weight, height and width. Uterus volume capacity was also determined, based on volumetric method of Kwaśnicki’s (1951) with own modification.
All pigs were divided into three groups in respect to age at slaughter: A – below 160 days (n=38), B – from 160 to 180 days (n=28) and C – above 180 days of age (n=14). The results were elaborated statistically computing the arithmetic means (x) for every traits and standard deviations (s). One-way analysis of variance ANOVA was performed. The significance of differences between age groups was estimated using Duncan’s test.
Calculations were performed with STATISTICA 8PL Software.
Obtained results are presented in the tables below. The most pronounced differences in the development of the reproductive tract are dependent on the age of gilts concerning only the uterus weight (P≤0.01) and uterus vagina-cervix length (P≤0.05). Gilts at age 160-180 days attained the full stage of reproductive tract development. Differences between the compared age groups of gilts dealing with the other morphometric traits
and ovary characteristics were statistically not significant. -
Improvement of black locust (Robinia pseudoacacia L.) growing under marginal site conditions in Hungary: case studies
129-133Views:339In Hungary, black locust (Robinia pseudoacacia L.) is considered as an important exotic stand-forming tree species growing mostly under unfavourable ecological conditions for forest management. Due to climate change effects, its importance is increasing in many other countries, too. As a result of a selection programme, new black locust clones were tested in clone trials. Juvenile growth of 12 micropropagated black locust clones in two plots series established at different dates were evaluated in central Hungary under marginal site conditions. At age of 7 the clone R. p. ‘Bácska’ (‘KH 56A2/5’), at age of 10 the clones R.p. ‘Homoki’ (‘MB17D3/4’) and ‘PV201E2/4’ appeared to be especially promising for mass production. Based on the data obtained from the performed trials, it can also be concluded that tissue culture can be considered as a suitable tool for propagating superior individuals and offers new prospects for the rapid cloning of selected genotypes used for plantation forestry.
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Comparison of the geranium (Pelargonium) pathological results of 2016-2017
123-125Views:122The research was carried out in a Gyenes Flower gardening between 2016 and 2017 in Kecskemét. The gardening was founded in 1978. Initially, the main plants were gerbera (Gerbera) and yucca (Yucca), later replaced by the geranium (Pelargonium) cultivation as a result of market demand. In horticulture, there are about than 80 variety geranium of the standing, running, semi-trailer types and English gnawing. The Pelargonium had different sizes and colors. The study was set up in 1,000–1,000 pieces of geraniums each year. The following pathogens have damaged the geranium stock: Botrytis cinerea, Pythium debaryanum, with a rare occurrence of Alternaria porri, Phytophthora cryptogea. The greatest destruction was caused by botrytis (Botrytis cinerea). In the first experimental year, 42% of the 1,000 geraniums tested were infected with fungal diseases (30% B. cinerea, 8% P. debaryanum, 4% other fungi). In 2017, fungal infections were detected on 380 geraniums in the 1,000 tested geraniums (290 Botrytis cinerea, 70 Pythium and 20 other fungal diseases). In addition to the use of fungicides, we increased the spatial position of geraniums, early irrigation and frequent ventilation to ensure successful control. By 2017, we were able to reduce the damage caused by pathogens by 4 percent.
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The influence of fertilization on the soil characteristics of a calcareous chernozem in a long term experiment
47-52Views:82In the long term fertilization experiment of the University of Debrecen, Centre for Agricultural and Applied Economic Science(CAAEC) (Debrecen Látókép), the effects of a 25-year-long fertilization were examined in terms of some chemical and microbiological properties of soil. With the growing doses of fertilizers, the available nutrient content of soil increased. At the same time the pH significantly decreased, while the hidden acidity increased. Moreover, the ratio between the soil bacteria and microscopic fungi, and the occurrence of microbes also changed. The number of sensitive physiological bacteria groups decreased dramatically. These changes indicate the reactions of living organisms; they correspond to the „resistance stage” of stress effects, but in the case of nitrifying bacteria, they reach the „exhaustion stage”.
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A study of social and economic influences of universities in the Derecske-Létavértes subregion
153-157Views:106Businesses operating in the region – adopting to local conditions – mostly pursue agricultural activities. Their R+D activity is rather moderate and largely focuses on development, moreover, the range of innovative businesses is relatively significant, which businesses – before long – will become potential partners for institutions of higher education (especially for Debrecen University).
Social impacts of universities are notable on the person’s status in the labour market. Our study of the Derecske-Létavértes region shows that those with a university degree have an advantageous position in the labour market, compared to the sample of youth aged between 16 and 35. Knowledge obtained at college and other skills greatly help the young to find employment.