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The significance of local meteorological stations in research planning
87-91Views:419The goal of research planning is to optimize human and material resources while maximizing efficiency. If there are databases that can be used as a substitute for own data collection, effective research can be facilitated by reallocating resources. In the case of environmental studies, the knowledge of the climatic conditions of the study period is known to be a key factor in research planning process.
In the present study, the data of our meteorological station in our research area (known as “Szamárháti Tanya”, Kesznyéten, Hungary) was compared with the measurements of meteorological stations operated by the competent water authority. Stations were taken into the study within a 10 and 20 km radius over a period of 21 months, to determine which provided more relevant data from the area.
During the evaluation of results, the relationship between the local and regional weather stations were determined, and deductions were made from the obtained results to support the decision which of the targeted investigations could be of greater benefit.
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Measuring of nitrogen leaching using ceramic suction cups at different locations
10-17Views:206Ceramic suction cups were used for the measurement of N-concentration in soil solutions under different soil and climate conditions in both field experiments of Rostock University and Agricultural University of Debrecen (Hungary). Depending on the soil utilisation the change in the N concentration of the soil solution can be proved on both sites.
The experimental field of Rostock University can be characterised by its high groundwater table. The nitrogen concentration of soil solutions in the different soil layers were determined by the trend downward of water. In the dactylis (Dactilis glomerata) experiment, the quadruple treatments involved the following: with and without N-fertiliser, with and without harvesting, respectively. In the lower soil layers, the least rising N concentrations were established in case of the treatment without N-fertiliser combined with harvesting. The nitrogen leaching calculated from the infiltrated water quantity and the nitrate N concentration increased in the following order: without N-fertiliser, with harvesting < without N-fertiliser, without harvesting < with N-fertiliser, with harvesting << with N-fertiliser, without harvesting.
The field experiment site of Debrecen can be characterised by a low groundwater table. The effect of N-fertilisation on the nitrate-N concentration of soil solution in the soil layers can be stated unanimously. Permanent nitrate-N leaching cannot be established due to the water upward movement under semiarid climate conditions. Intermittently transfer of nitrate-N between the soil layers is probable in cases of remarkable precipitation. -
Development of technology elements for growing of perennial sorghum
15-17Views:225Optimal sowing time for perennial sorghum under irrigation is when soil temperature at the depth of sowing reaches up to 10–11 °С, harvesting for the green mass has to be done when panicle is situated on the stem in 10 sm from the flag leaf and hight of cut must be 11 sm.
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Characterization of two rust fungi related to biological control concept in Hungary
195-199Views:585Weeds cause serious problems in agriculture on a global scale. These plants reduce yield and the quality of crops by competing for water, nutrients and sunlight. The improper or excessive usage of herbicides have led to development of resistance in some weed species while contaminating the environment; therefore, biological control has an increasing role as an alternative method for controlling special weed species.
The aim of this study is to make a brief review of biological control of weeds by pathogens and to characterize two rust fungi (Puccinia lagenophorae and Puccinia xanthii) which are broadly examined recently in a biological control concept and have been found on their hosts, such as common groundsel (Senecio vulgaris L.) and common cocklebur (Xanthium strumarium L.), two common and difficult to manage weeds both in horticultural and agricultural lands also in Hungary.
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Comparative study of newly-bred black locust clones with regard to photosynthetic rate and water use efficiency: early evaluation
5-10Views:585Black locust (Robinia pseudoacacia L.) is one of the most important tree species in Hungary, due to its positive economic impacts. Research to increase its yield, improve its stem quality and enhance its drought tolerance has been ongoing since the 1960s. Of the current research works in this field, the clone trial of the Forest Research Institute, University of Sopron, established in 2020 in the Nyírség region, is worth highlighting. In this experiment 4 newly-bred clones and a state-approved black locust cultivar ('Üllői') are being tested. In the summer of 2022, ‘on site’ measurements of assimilation parameters – net assimilation (An), transpiration (Tr) – were carried out using the LI-6800 portable photosynthesis system. From the data obtained, the water use efficiency (WUE) was calculated. The results of the statistical analysis (Kruskal-Wallis H test) have shown significant differences (p < 0.05) between the clones for all three parameters (An, Tr, WUE) tested. The NK2 clone has performed the highest value for all the parameters studied. However, no significant differences were found between clones NK2 and PL040 for Tr or between NK2 and control ('Üllői') for WUE. Studies of this kind contribute to the improvement of black locust growing through the production and selection of cultivars, which are relatively resistant to the negative effects (drought) of climate change.
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Examination of compost maturity using reflectance
29-34Views:626Composting is one of the most popular recycling processes for organic waste. Composting plays an important role in waste and by-product management and is becoming increasingly important in both sustainable energy management and circular economy. Composting transforms organic matter to produce a safe and stable by-product (compost) that can be applied to arable land in a similar way to fertilizer. Physical, chemical and biological methods can be used to monitor the process and to determine the maturity of the compost, as spectrometric/spectroscopic methods play an important role in the analysis of different environmental samples.
Our aim was to (1) non-destructively detect the effects of different additive ratios on the spectral properties of the composting process and the spectral data of different compost mixtures, (2) to find the wavelength ranges of the reflectance curve (inflection points) sensitive to compost maturity, (3) to determine the correlation between the inflection points and the chemical and physical parameters measured in compost by conventional methods.
The mixture of broiler and hen manure and zeolite was composted 62 days in windrow composting. In the composting experiment, the moisture content and temperature (°C) were measured every three days and compost samples were taken and in 10% destillated aquaeous suspension were measured the pH and electrical conductivity (mS cm-1). Compost samples dried to mass stability were spectrally analyzed in the wavelength range 400–1000 nm with AvaSpec 2048 spectrometer.
Based on the results, the reflectance of mature compost were smaller in the last days of composting than the reflectance values of day 0 samples, thus compost maturity can be detected spectral in the VIS-NIR wavelength range. For the tested compost prisms, the reflectance of each sampling day shows a constant slope, with a significant overlap of the reflectance curves up to 400–700 nm wavelength range, and there was a breakpoint in the 700–750 nm wavelength range which was proved by binary encoding.
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Evaluation of striptillage and conventional tillage in maize production
37-40Views:377Tillage changes soil properties and the way how the environment affects those properties. Soil properties and environment determine the rate of water movement in liquid and gaseous form into and out of soil. Based on the experimental database of the Institute of Land Utilisation, Regional Development and Technology of the University of Debrecen, Faculty of Agricultural and Food Sciences and Environmental Management and the KITE PLC, various cultivation systems were examined with maize (Zea mays L.) as indicator plant in Jász-Nagykun-Szolnok county. The sample area can be found in the outskirts of Kenderes on a meadow chernozem soil. On the examined plot, strip-tillage, subsoiling and moldboard ploughing were performed, each on 4.5 ha, respectively. The purpose of the present study is to compare these cultivation systems according to the soil- and maize kernel moisture content and to the yield based on the years of 2012 and 2013.
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Influencing the growth kinetics of yeast strains with vitamin supplementation
113-115Views:292The aim of the current experiment was to optimize the creation process of single cell protein on plant-based substrate solution with the intention to improve end-product turn out by means of adding vitamin solution. Based on the results of the fermentation processes of yeast strains, it was concluded that the vitamin-supplementation produced its greatest effect on the dry matter production, primarily on the K. marxianus DSM 4908 strain, while it was less beneficent when it comes to the figures of wet cell mass. In addition, it can be assumed that vitamin supplementation increased the maximum specific rate of growth (μmax) and decreased the generation time (tg) significantly. In the case of the K. marxianus yeast strain on corn steep liquor treated with vitamin-supplementation, the highest (μmax) and the lowest (tg) data were observed [(0.226 h-1) and (4.4 h), respectively]. Based on the results it was found that K. marxianus DSM 4908 is expedient to be applied on corn steep liquor medium in order to determine its suitability to produce additive for feeding.
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A review on fish in vitro digestion studies
55-63Views:150(1) Background: In vitro experiments in fish have been developed to search for dietary substitutes of fish meal in aquafeeds by measuring the digestibility of various feedstuffs by simply simulating the conditions of the stomach or portions of the digestive tract. In vitro digestion studies involving mainly commercial fish species have been conducted to determine the digestibility of conventional and alternative ingredients in fish diets using diverse digestion models. However, there remains a significant knowledge gap, particularly regarding the enzyme functionality, digestion mechanisms in many fish species, and the factors influencing enzymatic activity. This review article is focused on the importance of the use of different enzyme sources in the in vitro digestion model to predict protein hydrolysis.;(2) Methods: For this review, a comprehensive analysis of articles was conducted to gain insights into diverse enzyme interactions and feed evaluation assessments, from the nutritional science and biotechnology sector.; (3) Results: A total of 36 peer-review papers, which include original, and review articles were selected.; (4) Conclusions: In vitro digestion studies offer a valuable tool for evaluating the digestibility of conventional and alternative feed ingredients, helping to identify sustainable dietary substitutes for fish meal while reducing dependence on live animal trials.
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Baobab (Adansonia digitata L.) as a remedy to human livelihood challenges: a review
5-11Views:539Baobab (Adansonia digitata L.) is a unique plant predominant in different ecosystems in Africa. This review assessed its value in relation to human nutrition, health, and related aspects. Our synthesis shows that baobab leaves, seed oil, and fruit pulp were utilised in different forms. Baobab tree products such as fruit pulp, leaves, and seed oil contain high levels of macronutrients, micronutrients, vitamins, carbohydrates, and proteins. Therefore, promoting the utilization and processing of baobab products for improved human nutrition is key. However, the determination of optimal parameters through elemental traceability as well as the relationship between geographical locations and nutrient composition of the baobab plant is required. Altogether, promoting innovative utilization of baobab products by exploring processing techniques to develop novel food and product formulation from Adansonia digitata tree products sheds a green light to sustain human livelihoods.
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Storage of wheat at high moisture
111-114Views:194Deterioration rates were determined for 15-19% moisture content wheat (Dropia cultivar) stored at constant temperatures.
Deterioration rates were determined by measuring germination capacity of the grain and respiration rates of grain. Safe storage
time was defined as the time for germination to decrease to 90%. Safe storage times of 19% m.c. wheat stored at constant
temperatures ranged from 2.5 d at 30 and 35°C to 37 d at 10°C. Deterioration rates of 19% m.c. wheat stored with a step decrease
in storage temperatures (35-25, 30-20, 25-20, and 20-15°C) were determined and safe storage times were satisfactorily predicted.
Safe storage times of 17% m.c. wheat were 5, 7, and 15 d at 35, 30, and 25°C, respectively. Respiration rates and germination
percentages of 15 and 16% m.c. wheat stored at 25°C remained constant for 70 d. The respiration rates of 17-19% m.c. wheat at
25°C increased while the germination percentages decreased with storage time. Germination dropped from 98 to 92-89% when the
dry matter losses were about 0.05% and visible mould occurred when the dry matter losses were about 0.1% in 17-19% m.c.
wheat. -
Effect of Moringa leaf extract and set size on the bulb weight, diameter and yield of onions (Allium cepa L.)
127-131Views:719Studying the use of natural extracts or biostimulants in improving vegetable plants is the current needs as an alternative way to the use of chemical products. This experiment was carried out to evaluate the effect of Moringa oleifera leaf extract (MLE) as a natural growth regulator on bulb diameter, weight and yield of onions ‘Texas Early White’ cultivar. Therefore, three concentrations (control, 2%, 4% and 6%) of MLE and three different onion set sizes [small (3±1 g/set), medium (6±1 g/set), and large (10±1 g/set)] were prepared in a randomized complete block design with three replications and plant spacing 20×15 cm. MLE was applied as a foliar spray directly onto the plants with the amount of 25 ml/plant every two weeks from sprouting to maturity. The achieved results indicate that the interaction of foliar spray with moringa leaf extract 4% and large set size of 10±1 g/set gave the highest value of the total yield 4802.7 g m-2, while the lowest yield 1531 g m-2 was recorded in the interaction of control and the smallest set size of 3±1 g/set. Also, a significant difference was recorded in bulb diameter separately. Thus, the largest bulb diameter 68.90 mm was obtained from the planting of the largest set size 10±1 g/set with 4% of MLE while the smallest bulb diameter 38.40 mm was recorded from the smallest set size 3 ±1 g/set with control treatment. Similarly, a significant effect was recorded in both set size and MLE separately. The highest bulb diameter 58.71 mm was achieved in the planting of the largest sets 10±1 g/set, while the lowest diameter was 42.12 mm in the planting of the smallest set size 3 ±1 g/set. Similar results were recorded for the bulb weight and total yield. The concentration of 4% MLE produced the highest bulb weight 99.74 g/bulb and yield 3324.5 g m-2, while the lowest bulb weight and yield was obtained in the control treatment 55.61 g/bulb, and 1869.3 g m-2. Consequently, the results indicate the positive effect of Moringa leaf extract which can be applied as a natural substance in the form of foliar spray at critical growth stages to improve the growth and yield of dry onions.
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Impact of ammonium nitrate and Microbion UNC bacterial fertilizer on dry matter accumulation of ryegrass (Lolium perenne L.)
35-39Views:201Pot experiment was performed to investigate the effects of increasing NH4NO3 doses with or without Microbion UNC bacterial fertilizer
application on dry matter production of ryegrass (Lolium perenne L.). Experiment was set up on calcareous chernozem soil of Debrecen-Látókép and on humus sandy soil of Őrbottyán. The bi-factorial trials were arranged in a randomized complete block design with four replications. Grass was cut three times. Dry matter production was determined and the sum of biomass of cuts was calculated as cumulated dry weights. Analysis of variance was carried out on the data in order to provide a statistical comparison between the treatment means. The least significant difference (LSD5%) test was used to detect differences between means. On the basis of our results it can be concluded, that the dry weights of ryegrass cultivated on chernozem soil were higher than on sandy soil. With increasing nitrogen supply the dry matter production of grass significantly increased in both types of soils. In case of sandy soil the increasing effect was more expressed, but dry weights of this soil never reached the appropriate values of chernozem soil. Application of Microbion UNC had positive effect on dry matter production of ryegrass grown on both two types of soils but the effect was more expressed on chernozem soil. Finally it can be concluded that the increasing effect of NH4NO3 on biomass weights was more expressed in both types of soils, the biofertilizer application also increased the dry weights of plant in a small degree. -
Modern approaches to assessment of PAHs bioavailability in soil for environmental toxicology research
35-36Views:204The efficiency of TENAX beads sorption method enabling to separate the bioavailable fraction of PAHs in the soil has been estimated. Due to the method the PAHs effects on soil biota have been explored. Laboratory investigations have ascertained possibility of application of TENAX beads sorption for soil quality assessment.
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Effect of agrotechnical factors on the yield of the Grolim asparagus (Asparagus officinalis L.) hybrid on acidic sandy soil
43-48Views:519Research of blanched asparagus has begun at the University of Debrecen Institutes for Agricultural Research and Educational Farm Research Institutes of Nyíregyháza in 2011. Establishment of the plantation took place in May 2011. The Grolim hybrid was used in the trial, 16 medium plot trial area has been formed under field conditions, with four repetitions and 36 m2 plot size. In the course of our studies, the effect of different nutriment supply methods (untreated, manure, sheep manure compost, fertilizer) has been analysed on the spear yield of the Grolim asparagus hybrid between 2013 and 2017.
In our studies, the beginning of vegetative growth has been recorded upon the constant presence of 10 °C of average soil temperature in the case of the Grolim asparagus hybrid. The beginning and length of spear harvesting are both influenced by the time and dynamics of initial development in spring. During the analysed period, the dates of spear harvesting were various, the earliest being on 23rd March, 2014 and the latest on 23rd April, 2015; the rest of the three years have been varied within this one month interval. The total of heat units required for the vegetative development of spears has been determined; it provides important information for cultivation practice.
Spear yields turned out to be hectic during the analysed period. In 2013 and 2014, yields have surpassed the amount of 50 kg/harvest period/plot in the case of every treatment version. However, in 2015 a significantly lower specific yield has been recorded due to the unfavourable weather conditions in spring; a yield decline of nearly 50% was recorded in the case of the control treatment compared to the previous years. Yield was also lower in the rest of the fertilization treatments compared to 2014; however, in these cases, the degree of yield decrease was around 5–10%, which suggests the yield stabilising effect of fertilization. In 2016, a slight yield increase was measured in comparison with the base year. In 2017, there was a decline of yield in the control treatment; however, the different fertilization treatments resulted in yield increase as compared to previous years.
On the basis of our studies, it is clear that the best yield results have been provided by the artificial fertilization treatment in all of the five analysed years. It was followed by the sheep manure compost and manure treatments in terms of their effect on spear yield. During the three harvesting periods, the lowest yield on acidic sandy soil was recorded in the case of the control treatment. The most remarkable effect of nutriment treatments has been realised in terms of the decreased deviation of yield results, which perfectly represents the yield stabilising effect of nutriment supply in the case of perennial crops – asparagus – as well, even on a poor nutriment supply characteristic sandy soil.
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The Examination of the Effects of Value Modifying Factors on Dairy Farms
36-40Views:166We wish to present a method to quantify the value modifying effects when comparing animal farms. To achieve our objective, multi-variable statistical methods were needed. We used a principal component analysis to originate three separate principal components from nine variables that determine the value of farms. A cluster analysis was carried out in order to classify farms as poor, average and excellent. The question may arise as to which principal components and which variables determine this classification.
After pointing out the significance of variables and principal components in determining the quality of farms, we analysed the relationships between principal components and market prices. Some farms did not show the expected results by the discriminant analysis, so we supposed that the third principal component plays a great role in calculating prices. To prove this supposition, we applied the logistic regression method. This method shows how great a role the principal components play in classifying farms on the basis of price categories. -
The impacts of spring basal and side dressing on maize yield
83-86Views:340The yield potential of maize is very high. According to Tollenaar (1983), maize yield potential is as high as 25 t ha-1 (absolute dry yield) which is the highest among all cereals. In order to fully utilise this high yield potential, proper nutrient replenishment is of chief importance among all agrotechnical factors.
The aim of research was to examine the effect of nitrogen fertiliser applied as basal and side dressing on maize yield.
The measurements were performed at the Látókép experiment site (47° 33’ N, 21° 26’ E, 111 m asl) of the Centre for Agricultural Sciences of the University of Debrecen on mid-heavy calcareous chernozem soil with deep humus layer in an established experiment in 2011, 2012 and 2013. The trial design was split-split-plot with two replications.
Based on the experiment results, it can be established that the nutrient uptake of maize is greatly dependent on the amount of water store in the soil. From the aspect of the development of the maize plant and water supply, the most determinant factor was the distribution of precipitation over the growing season and not the amount precipitation. This is shown by the fact there was only 276 mm precipitation – which was favourably distributed – in 2012 to increase the availability of nutrients and the main average was the highest in this year (14.394 t ha-1).
Spring basal dressing helped maize development in all three years even on chernozem soil which is well supplied with nutrients. Although the effect of side dressing did not result in any yield increase, it could still contribute to mitigating the stress effects caused by environmental factors. Altogether, nutrient supply adapted to the various development stages of maize can favourably affect the success of maize production.
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Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
153-157Views:469Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.
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Possibilities of mass valuation in land use in Hungary
59-68Views:362Technological development makes it possible to simplify and accelerate decision-making processes by adequately processing and evaluating large volumes of data. Sub-data obtained from large data sets have a very important practical role in asset valuation, forecasting and valuing delineated or difficult-to-map areas, or in the context of portfolio management. Land valuation is a separate segment within asset valuation and it requires a specific methodological approach on behalf of evaluators. In this study, the authors compared the transaction data of arable land and the value of other land use categories. Based on empirical assessments, the authors developed proposals for the fast and cost-effective determination of the value of land use categories other than arable land - mainly meadows and pastures.
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Development of a New Maize (Zea mays L.) Breeding Program
25-30Views:321Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.
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Comparison of growth parameters of 40 varieties/clones short rotation coppice willow (Salix)
99-104Views:242The plantation of willow varieties was established in 2009. The 40 different varieties and clones of Salix were planted at a research field in Kolíňany (Nitra district). The paper evaluates results from the first growing season in 2009. The survival rate of planted cuttings ranged from 55.56 % to 100.00 % after the first year. The lowest survival rate was reached by Terra Nova variety. The stem numbers per plant ranged from 1.17 ±0.37 to 2.53 ±0.98. The average height of one-year old stem varied from 65.82 ±36.60 cm to 225.58 ±68.61 cm. The average stem diameter ranged from 6.90 ±2.63 mm to 14.34 ±3.39 mm. There were statistically very significant differences in parameters of stem height stem diameter and stem numbers per plant among studied varieties/clones. The statistic method used was analysis of variance ANOVA. The varieties were then divided into 6 groups according to their similarity in observed parameters after the first growing season by cluster analysis. The best results were reached by varieties/clones classified in the second group. The survival rate, stem diameter and stem height values of these varieties/clones were above average.
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Emerging sectors in rural development
65-72Views:241This paper focuses on the innovativeness of rural economy and in particular on the way rural entrepreneurs in endogenous and exogenous sectors make use of their rural environment. building upon the sustainable innovation framework, key strategies for rural development are formulated. the described data refers to the rural innova region (10 rural eu regions). The results show that rural innovation is essentially a process of valorising endogenous potentials by connecting them with exogenous assets.
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Evaluation of water balance in apple and pear trees
193-198Views:609A significant proportion of the aboveground green and dry weight of the plant constitutes the foliage. The canopy is an important factor
of plant growth. On one hand the canopy absorbs the solar energy, which is necessary for the photosynthesis, on the other hand accumulates
the absorbed nutrients by the roots, and the most of the water-loss happens through the foliages. The determination of the full canopy is not
an easy target. In our research we developed a measurement method to determine the leaf area. With the parameters of the examined tree
(leaf length and maximum width) and the data of ADC AM 100 leaf area scanner we determined the k-value, with which we can easily and
fast evaluate the leaf surface. Furthermore we defined from the water balance of compensation lysimeters the cumulative transpiration of
fruit trees and the efficiency of water use of trees. -
Folic acid content of beetroot leaf and root by different growing stages and genotypes
115-119Views:529An increasing interest has been observed of beetroot leaf as a salad component due to recent studies focusing on their nutritional value. The randomized field experiment was carried out on lowland chernozem soil with 6 varieties, 3 replications and 2 sowing dates. Sampling was performed on 23 of August 2018 at the stage of 30 and 50 days of vegetation, where leaf (30 and 50 days) and root (50 days) were collected. Total dry matter, folic acid and nitrate content were evaluated.
The results of this investigation show that higher total dry matter content was measured in the root (8.47–10.30%) compared to the leaf in both developmental stages (6.47–9.20%). Nevertheless, higher folic acid content was found in the young leaves of 30 and 50 days of development (58.77–113.86 µg 100g-1). Among the examined varieties, Bonel has presented great amount of folic acid not only in the leaves (99.35–113.61 µg 100g-1), but also in the root (89.99 µg 100g-1). Finally, lower nitrate content was found in Libero (316.16 mg kg-1) at 30 days and in Akela (340.41 mg kg-1) at 50 days of development. Thereby, fresh consumption of beetroot leaves are highly recommended.
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Mitochondrial DNA-based diversity study of Hungarian brown hares (Lepus europaeus Pallas 1778)
23-29Views:352The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.
The aim of our large scale long term ongoing study is to fill this gap of information on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.
This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.