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The Effect of Fertilization and Irrigation on Maize(Zea mays L.) Production
26-29Views:110In a long-term field experiment set up at the Látókép experimental station of the Center of Agricultural Sciences of Debrecen University, the data of the last five years (1995-1999) were analyzed to determine the crop production factors with the greatest influence on maize production and the relationship and interactions between irrigation and fertilization.
In the extremely dry year of 1995, fertilization was found to cause substantial yield depression in the absence of irrigation. According to results of analysis of variance, fertilization significantly reduced the maize yield by 40-90% compared to control plots. Under irrigated conditions, there was a considerable increase in the maize yield, the yield surplus being 4.4-9.4 t ha-1, depending on the nutrient supply level.
During the period from 1996-1999, when rainfall conditions were favorable for maize, fertilization significantly increased the maize yield even without irrigation over the average of the four years. The yield surplus due to fertilization was 3.9-4.6 t ha-1, depending on the fertilization rates. The maximum yield surplus was obtained on plots fertilized with 120 N kg ha-1, while at the rate of 240 N kg ha-1 the maize yield did not differ significantly from this value. During the period examined, corn yield was significantly higher at all three nutrient supply levels as the result of irrigation than in the non-irrigated treatment. As in the case of non-irrigated conditions, the highest fertilizer dose did not result in a substantial yield increase. An analysis of the interaction between fertilization and irrigation indicated that the yield-increasing effect of fertilization was not significantly different under irrigated and non-irrigated conditions. The significant year x irrigation interaction was confirmed by the fact that the yield surplus (1.3-2.3 t ha-1) differed greatly from the irrigation effect recorded in 1995. -
Complex problem analysis of the Hungarian milk product chain
43-47Views:90Hungarian dairy sector went through significant changes in past two decades. The most significant changes were caused by our accession to the European Union. In Hungary milk production remarkably declined after EU accession. The size of our dairy herd has been practically reducing since the political transformation, but increasing yields per cow could compensate it in some way and for some time. However, in recent years, increasing yield per cow came to a stop and in parallel, the number of cows declined further and faster. Low prices, high production costs and tightening quality requirements ousted several producers from the market in past years. Feeding cost represents the highest rate in cost structure of production, but animal health expenditures and various losses are also significant. There are undeniably competitive disadvantages in the level of organisation and labour productivity; however competitiveness already depends on cost effectiveness in the medium run. In Hungary concentration of the dairies is relatively strong in spite of the relative high number of corporations. The dairies compete with each other and with the export market for the raw material and the better exploitation of their capacities. Applied technology of the Hungarian dairies lags behind the Western-European competitors’; in addition they have handicaps in efficiency and product innovation. Presence of chain of stores being dominant in sale of milk products does also not favour in all respects to the position of the dairies. The aforementioned retail chains are namely consumer-centric, engage in price follower conduct and weaken the position of the dairies with their private label products. As a result of increasing import of milk and milk products Hungary became a net importer in recent years. Today, disposable income still essentially determines the consumption habits of price-sensitive consumers. Loyalty for Hungarian products is not typical, consumers are open for import products being preferred by retail chains. In addition Hungarian milk and milk product consumption is about half of the Union average and it is far behind the level being necessary for healthy eating. In Hungary lack of competitiveness and vertical integration relationships and backwardness are revealing among the dairy farmers and the dairies, while chain of stores are in unprecedented “monopolistic situation”; the whole sector can be characterised by defencelessness.
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Morphological diversity of current melons (Cucumis melo) compared to a medieval type
84-90Views:103Morphological diversity of melon (Cucumis melo); phenotype reconstruction of a medieval sample. Morphological diversity among 47 melon (Cucumis melo) cultivars and landraces from Hungarian germplasm collection (ABI, Tápiószele) were analyzed with an ultimate aim to characterize morphologically cv. Hógolyó, which showed the closest genetic similarity to a medieval melon recovered from the 15th century. Cultivars based on fruit morphology were grouped into the three main types of melon as reticulatus, cantalupensis and inodorus. Cluster analysis (by SPSS-11) based on 23 morphological (quantitative and qualitative) traits recorded revealed an extreme diversity among accessions, nevertheless cultivars were clustered into main melon clusters with only two exceptions of inodorus type cv. Zimovka J. and Afghanistan. Cultivars Sweet ananas and Ezüst ananász; and two Hungarian landraces Kisteleki and Nagycserkeszi showed close similarity. Cultivars Hógolyó and Túrkeve of inodorus type
were also grouped in one cluster, which provide insight into the morphological reconstruction of the medieval melon recovered from the 15th century. These results also indicate that old Hungarian landraces could be re-introduced into breeding programs for broadening genetic base of melon. -
Examining the compensation for the 2010 flood in the Sajó valley using real estate examples
5-14Views:158The intention of this article is to investigate the central damage mitigation of the 2010 flood in point of properties. During the investigationI performed the processing of the revealed data on a country level perspicuity, and in details on the level of sample area. In my study I worked with the available damage summations, the number of the damaged properties and the sources granted by central budget to extenuate those,and the husbandry database comparative analysis of local government of the sample strata. It came clear by the analysis of the results, which regions require more acquisition in the further process of the project.
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Analysis of the photosynthetic parameters, the yield and the quality of winter wheat
101-106Views:125The environmental adaptability of crop production is basically determined by the selection of biological background (plant species and
varieties) suitable for the region and the site. The aim of our work is to parametrize the plant assimilation, its intensity, dynamics and the
most important characteristics and the relationships to the quality in winter wheat trials. The measurements were carried out at the research
site of the University of Debrecen in small parcel experiments. We measured the leaf net CO2 assimilation rate, stomatal conductance,
intercellular CO2 level, the transpiration, the leaf temperature and the air temperature by the LICOR LI-6400 portable photosynthesis
system in field trials on the nutrient supply. The soil of the experimental area is calciferous chernozem with favorable water regime.
We have examined the photosynthetic activity, the productivity and yield stability of winter wheat varieties. We have compared the yield
results, at similar agrotechnical conditions in seven cropyears. We also determined the quality parameters of the winter wheat varieties.
Then we valued the yield stability of genotypes with the help of analysis of variance and linear regression equations. We have defined the
connections between assimilation parameters, the yield stability and quality parameters of wheat varieties. -
Technological and Economic Analysis on the Hungarian Rice Sector
226-233Views:67Hungary lies on the northern edge of rice production area. According to this, the climatic conditions area not perfect for this species. The production area of rice involves typically the poorer quality soils, however these meet the requirements of rice. In Hungary exclusively domestic types are grown which have high yield and good quality and these are usually wore successful than foreign types. On the other hand, these Hungarian types should be improved considering safety in production. Nowadays, rice is grown in large scale companies with 300-1400 hectares, where production technology already exists, machinery is suitable, however the latter one a little bit old.
The average yields of the analysed companies were 3-4 t/ha in the past few years, which were a little bit above the national averages. The operating cost per hectare is almost 200 thousand HUF, from which the main part is the cost of machinery (35%) and the material cost (34%). The main part of the latter one is the irrigation costs (30%). The average cost, calculated from the total production cost, is 80 thousand HUF/t. Considering the above-mentioned costs and the price of rice (75 thousand HUF/t) it can be stated that the profitability of the rice sector is not the best, the cost rated profitability is -6.6%. According to the results of this analysis possibilities for the increase in profitability and improvement are increased subsidies and market price, as well as genetic improvement. -
Research of the interrelations between actors and strategic goals in decision making
71-75Views:53management, although there are many chiseled methodologies plenty of professional literature.
The problem is more complex in strategic planning of regional communities – societies – of different range. These societies are very diverse, and accordingly the strategic goal setting can, and has to be very different than the strategic goal setting of enterprises.
The group of methods for morphologic and structural analysis and the computer based MACTOR program could be used.
The output data are presented in a large number of matrixes, vectors, graphs and histogram’s, which describe very fair the possible scenarios for preparation and decision making about
strategic plans. -
Analysis of the Environmental Status of Nagykálló Subregion
362-367Views:83The third smallest region of Szabolcs-Szatmár-Bereg county is the Nagykálló subregion. Its territory is 377 km2 and its inhabitants number 32.526. Due to the fact that industrial development arrived late, the environmental status of this subregion was saved from serious ecological degradation. The quality of the environment shows a reasonable picture in many respects, comparing to the general survey of the country. The air quality of the region can be qualified as acceptable. Leaving some critical points out of consideration, it is better than the national average. It is favourable from the human environment point of view that the region is free of extremes, and has a balanced climate. The supply of drinking water is above 95%, and the remainsing water requirements are supplied by artesian wells. The water supply network is fully extended in the subregion. The most significant environmental noise source is traffic, including public road traffic, which causes a problem in the town of Nagykálló. The situation of the collection, transportation, and placement of the settlement’s solid wastes show a similar picture to the status of the country. The environmental status of the region is included in the SWOT analysis.
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Sensitivity of methods for estimating reference evapotranspiration
51-56Views:129The knowledge of the evapotranspiration of natural ecosystems and plant populations is of fundamental importance in several branches of science, research topics and practical uses. Nevertheless, the harmonization of the large number of methods and the changing user needs often causes problems. Sensitivity analysis of 10 ET0 estimation models and model variants was performed. Magnitude of the obtained outputs and the changes triggered by each atmospheric parameter were evaluated. The objective of the analyses was to get to know the sensitivity of the different models and to select the most accurate and the most suitable ones for adaptation to local circumstances. Therefore, it becomes possible to achieve as high accuracy as possible in applications which need ET0 estimation.
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Qualitative detection of genetically modified organisms in plant samples
309-313Views:93We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.
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How can we determine the expected prices for lambs? – Analysis of the Hungarian and Italian lamb prices
59-67Views:65The Hungarian sheep sector has become a one-market sector, almost the whole amount of slaughter lamb went to Italy, which increased its defenselessness. In addition to the sole Italian market showing a permanent demand for Hungarian lambs, it would worth to exploit possibilities in other European markets considering the seasonal differences in Italian prices. Such markets can be the Spanish and Greek for ”light” and the French, German and English markets for ”heavy” lambs. Both the Italian and Hungarian prices have three major periods: Easter, Ferragusto and Christmas. Due to the changes in the prices, the producers often suffer great losses, therefore, the temporal adaptation of production, market research, technological and development activities need to be improved. It is important that the living of Hungarian sheep breeders should not be dependent solely upon the Italian market, distribution of products should be ensured in other markets too, so that the safety of sales be increased and higher prices and higher income could be achieved. The European lamb prices are characterized by large seasonal fluctuation and the degree and timing of changes are different in the different countries. Study of the literature and the results obtained in my study on lamb sales called for an analysis and study of the possibilities of price forecasting. In my study, I performed a forecasting of lamb prices in Hungary and Italy for the period between 1996 and 2006 based on the data of the European Committee. Among the forecasting methods, seasonal decomposition and SARIMA models are the most precise, producers can achieve a better market position by using these in the practice.
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The use of MFA indicators in the characterization of the settlement sustainability
107-116Views:68One of the key sustainability challenges for the coming decades will be to improve the management of natural resources in order to reduce current levels of anthropogenic environmental pressure and respect the biological and physical limits and the carrying capacity of the planet. The first step towards meeting this challenge is an enhancement of the understanding of the material basis of our society. In the past 15 years, scientists in several research institutes have created a fast growing field of research, a new family of different methods, named material flow analysis (MFA). These instruments have an increasing policy relevance: international organizations (UN, EU, OECD) have encouraged member states to establish MFA accounting in their statistical programmes and urged governments and economic actors to use these tools. In this paper I present selected examples to reveal how the MFA approach and derived material flow indicators can be used for the evaluation of sustainability policies at municipality level.
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Examination of renewable energy sources and their endowments
67-70Views:93Due to the exhaustion of fossile fuel reserves, the increase of their prices on the world market and the environmental pollution resulting from their use make alternative energy sources increasingly important. In my study, I am going to present the currently known and researched renewable energy sources. Based on the results achieved from the SWOT analysis, I provide an overview on the future opportunities of their use. After the comprehensive examination of alternative energy sources, I give insight into the factors which influence the energetic use and utilisation of biomass, as well as the theoretical background of bioenergy. In my work, I pay particular attention to the analysis of the use of biomass as an energy source, as well as the difficulties related to its utilisation.
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Examination of motivation in civic organizations
73-79Views:98The primary objective of my research is to examine and explore the specialties of the civil organizations composition and operation. In my study I describe the results of my research in Hajdú-Bihar County. The results concern to the features of motivation and incentive in civil organizations.
Quantitative data from an empirical analysis is used for the research. My questionnaires were measured on a representative sample. The basis of this sample was the County Court of Justice’s website. Thus, my research on managerial tasks included in Hajdú-Bihar County. During the survey I examined the motivation from different sides. First of all, what was the purpose of establishing civil organizations? I also analysed the managers’ motivation. Why they participate in civic activities? Finally, I examined the perception of managers of incentive forms. The general findings beyond the context of the investigations were carried out on the basis of organizational parameters and characteristics of the interviewees.
The results of my research arise from an analysis of data I collected. Civil organizations are established for a well-defined task or as a way of problem-solving. In terms of internal motivation of managers stated that it was mainly an internal urge and desire to help the cause, participate in the activities of civic organizations. In their motivational activities, the psychological incentives were classified as most effective motivators.
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The effect of the queen's age on the Varroa mite (Varroa destructor) burden of honey bee (Apis mellifera L.) colonies
83-87Views:275An apiary trial was conducted in 2016 August to October in Szabolcs-Szatmár-Bereg County, Nyírmada to evaluate the influence of queen’s age on the Varroa destructor-burden in the treatment colonies. Sixty colonies of bees belonging to the subspecies Apis mellifera carnica pannonica in Hunor loading hives (with 10 frames in the brood chamber/deep super) were used. The colonies were treated with amitraz and the organophosphate pesticide coumaphos active ingredients. The amitraz treatment includes 6 weeks. The coumaphos treatment with Destructor 3.2% can be used for both diagnosis and treatment of Varroasis. For diagnosis, one treatment is sufficient. For control, two treatments at an interval of seven days are required. The colonies were grouped by the age of the queen: 20 colonies with one-year-old, 20 colonies with two-year-old and 20 colonies with three-year-old queen. The mite mortality of different groups was compared. The number of fallen mites was counted at the white bottom boards. The examination of spring growth of honey bee colonies has become necessary due to the judgement of efficiency of closing treatment. The data was recorded seven times between 16th March 2017 and 19th May 2017.
Data on fallen mites were subjected to one-way analysis of variance (ANOVA) and Post-Hoc Tukey-test. Statistical analysis was performed using the software of IBM SPSS (version 21.). During the first two weeks after treatments, the number of fallen mites was significantly higher in the older queen’s colonies (Year 2014). The total mite mortality after amitraz treatment in the younger queen’s colonies was lower (P<0.05) compared to the three-year-old queen’s colonies. According to Takács and Oláh (2016) although the mitemortality tendency, after the coumaphos (closing) treatment in colonies which have Year 2014 queen showed the highest rate, considering the mite-burden the colonies belongs to the average infected category. The colonial maintenance ability of three-year-old queen cannot be judged based on the influencing effect on the mite-burden. The importance of the replacement of the queen was judged by the combined effect of several factors.
During the spring-growth study (16th March–19th May) was experienced in the three-year-old queen’s colonies the number of brood frames significantly lower compared to the one- and two-year-old queen’s colonies. In the study of 17th April and 19th May each of the three queen-year-groups were varied. Therefore in the beekeeping season at different times were determined the colonial maintenance ability of queens by more factors: efficiency of closing treatment in early spring, the spring-growth of bee colonies, the time of population shift (in current study, this time was identical in each queen-year), honey production (from black locust).
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Comparison of the sample preparation methods worked out for the examination of the element content of wine
77-82Views:113The examination of the potentially toxic elements content of the wines is not easy task, because the most elements are in little concentration (mg kg-1 or μg kg-1) in the wine and the wines contain great amount of organic matrix. The efficient sample preparation is essential for the accurate determination of element content. The eim of our research was to determine which sample preparation method will be the most efficient in examination of wines with ICP technology. The examined wine sample was a 2008 Chardonnay from the Eger wine region. We did the sample preparation and analysis examination in University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Institute of Food Science, Quality Assurance and Microbiology.
We did the analysis examinations with ICP- MS (inductively coupled plasma mass spectroscopy). We always did the sample preparations and the examinations in three times rehearsal. The applied sample preparation methods: dilution with distilled water, open digestion and microwave digestion.
We were able to measure B, Al, Mn, Fe and Zn with only dilution and open sample preparation. In the smaller quantity present Sr and Ba were measurable in the wine in the case of all three methods well. We were able to measure the Co with dilution and open digestion method, while Cr, Ni, and Te with only dilution method. In the case of arsenic we were not able to measure reliable result with dilution and open digestion method because of organic matrix and other components
(alcohols, monosaccharides, polysaccharides, polyalcohols and inorganic salts). On the whole we are able to say that in the case of certain elements (B, Mn, Fe, Zn, Sr, Ba) the open digestion and dilution sample preparation is applicable well, however, in the case of certain elements (As, Al, V, Cr, Se, Mo, Cd, Hg, Pb) we have to develop the methods. It may be development of one of the way, if we develop sample preparation methods to examined element specifically and not
to wine generally. -
Interaction of yield stability and year in major agricultural crops
41-46Views:85The effect of hydro-meteorological extremities on plant cultivation is the result of the correlation of many factors. These may increase or decrease the effects of hydro-meteorological extremes. The degree of this variance depends on the professionality of treatments, on the quality of the applied technique and technology and also on the soil’s water management characteristics.
The water management characteristics of Hungary’s arable land are mainly unfavorable or medium. In the past two decades the conditions of originally good soils, from a water management aspect, have significantly deteriorated in the critical 0-60 cm soil layer. This is mainly due to unprofessional land use, a lack of deepening cultivation and neglected organic cultivation. At the same time, hydro-meteorological extremities occur more frequently and the sensibility of plant cultivation has increased.
The sensibility of plant cultivation is type and location specific, yet, it also effects both the quality and quantity of the result.
The stability analysis, which covered the period of four decades and incorporated 6-7 agro-ecological areas proves and highlights the following:
• Winter wheat only reacted to extensive cultivation and unfavorable environmental conditions to a small degree. On the other hand, the effect of hydro-meteorological extremities increases.
• The stability analysis of maize, which is sensitive to cultivation technology and the location of cultivation, proved just the opposite. Good soil and adequate technology significantly reduces the effect of any particular year.
• From the years examined, the most favorable proved to be the one with average precipitation. Maize reacted to both extremities in a similar way. Winter wheat reacted to more precipitation with less yield.
• The yield quality of winter wheat was negatively effected by drought. The negative effect of precipitation is limited to the period of ripening and harvest, so the likelihood of such an effect is not significant.
• The yield of sunflower – due to pests – significantly reduces in years with high precipitation, while a difference between dry and average years cannot be pinpointed out. The oil content in both dry years and in years with high precipitation is evident, compared to years with average precipitation.
• The root yield of sugar beet is reduced by drought while the sugar content depends on soil characteristics and climatic extremities. A difference could also be noted by location, whether in Western Hungary and on the Great Plain. Great sugar content can be achieved in years with high precipitation in Western Hungary, while the same result occurred with average precipitation on the Great Plain. Drought did not have a positive effect on sugar content in either location. -
Food allergy, safety and analytics
323-326Views:105The food allergy is a hypersensitivity reaction against naturally occurring proteins in food. These types of disease can cause not only personal inconvenience to the patient but serious health, food safety and food analysis, social-economic problems. The only effective treatment for these illnesses is a life-long diet avoiding the allergenic foods or components of food. In the interest of the patients’ health 14 allergenic components must be labeled on the food packaging. To meet the requirement of regulation reliable and valid analytical methods are necessary which for the most allergenic foods are not available.
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The effect of sowing date and plant density of winter oilseed rape (Brassica napus var. napus f. biennis L.) population
213-215Views:160The experiment has been set up in the University of Debrecen Látókép Experimental Station in three different years (2014, 2015 and 2016), three different plant densities 200, 350 and 500 thousand ha-1, four replications of the same nutrient supply with using a line spacing of 45 cm. In the experiment, the fore crop was winter wheat in each year. The amount of weeds was observed five times in the last experimental year (2016/2017). In the three experimental years, the highest yield was harvested from the early sowing plot with the highest plant density. On the basis of the Pearson’s correlation analysis there was significant negative correlation (r=-0.583) between the effect of the annual year and yield of the hybrid.
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Comparison of RAPD and AFLP Analysis in Some Maize (Zea mays L.) Lines and Hybrids
3-7Views:88The use of molecular markers to enhance plant breeding efforts is being widely studied. DNA-based fingerprinting technologies (RAPD and AFLP) have proven useful in genetic similarity studies. We estimated different maize (Zea mays L.) inbred lines and hybrids originated from mutant ones based on their genetic differences.
We carried out RAPD analysis with different primers and the 707 (CCCAACACCC) and 792 (CAACCCACAC) primers with 50% similarities provided quite good DNA fragments. By applying the DNA based-AFLP technique, we had very dense DNA fingerprinting. We differentiated 15-32 polymorphic bands, the highest number of bands were found in P-T/H-CA (32). AFLP seems to be the more efficient method of comparing genetic similarities/differences among different genotypes. -
Study of the economic involvement of civil organizations
139-145Views:98On the basis of definitions found in the literature, as well as my own, civil organizations are such structures that basically have specific organizational characteristics and their own management tasks in view of their operational principles.In my study, I describe the status quo of civil organizations, as regards their “economic involvement”. On this basis, I examined the level of the economic involvement of civil organizations, more specifically as to the level of economic partnership between representatives of civil organizations and greater society. I placed the economic involvement of civil organizations into three categories: ‘active’, ‘passive’ and ‘has no effect on individuals in society’. During my research, I largely build my investigations on the results I obtain through questionnaires, which I measure on a representative sample. On the basis of this sampling, the County Court of Justice was able to launch its own website. Thus, my research on this topic included the townships in Hajdú-Bihar County.
The results of my research arise from an analysis of data I collected in 2010–2011. This data allowed me to be able to make several observations concerning the specific activities of civil organizations. I have result of the organizations’ annual income, as well as the types of revenues they obtain the county. The results of my analysis of the examined organizations show that more than two-thirds (66.7%) of their available revenues totaled less than one million Hungarian Forints, with no significant difference in the amount of revenue existing between the two organizational types (association, foundation). The most common source of revenue claimed by the studied organizations was from membership fees and/or various types of funding programs. In examining civil organizations on the basis of their type, I made the observation that in the case of associations, it was the membership fees which were the most characteristic type of income, followed by the 1% donation possibility from personal income tax filings for foundations, but funding proposal possibilities were also important for both types of organization.
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Associations analysis of production traits with leptin gene T3469C polymorphisms in pig
39-43Views:138The aim of this study was to define the connection between the leptin (LEP) gene T3469C polymorphism and its potential association with production traits in improved hybrid pigs. The study included data from 397 gilts and barrows from 2 different sample. The polymorphism was identified by using the polymerase chain reaction-restriction fragment-length polymorphism (PCR–RFLP) method with HinfI restriction enzyme. Two alleles of LEP gene were identified: T (0.93) an C (0.07). The analysis of values of production traits, depending on LEP genotype did not reveal significant (P≤0.05) differences. In the examination the loin diameter (between the 2nd and 3rd ribs), the live weight at slaughter and the averege daily gain during fattening were higher at pigs with C allele than pigs with TT genotipe. Accordingt to our data the effect of C allele was favourable in this population, because these animals had bigger bodyweight without valuable change of lean meat percent.
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Application of microsatellite fingerprints for pedigree analysis of Hungaricum grapevine varieties
71-77Views:99the Carpathian Basin were involved into our examination, which aimed at genotyping their accessions. DNA fingerprints of 101 varieties were determined with 6 microsatellite markers till 2005, resulting in successful discrimination of the accessions. Based on these results for pedigree determination, even more cultivars and primers were involved into the analyses. For studying the origin of Csabagyöngye and for proving the parent-progeny relations of Irsai Olivér and Mátrai muskotály, 19 microsatellite markers were applied, while 11 were selected for tracing the origin of Királyleányka. Genetic distances between the varieties were estimated with cluster analysis and demonstrated by dendrogram, proving that the varieties can be discriminated from each other based on the microsatellite allele sizes. Pedigree of Irsai Olivér and Mátrai muskotály has been confirmed by microsatellite allele size results, searching for the parents of Csabagyöngye and Királyleányka is in progress, since the molecular-marker based pedigree does not correspond with the putative origin of these cultivars. Our results excluded progeny-parent relationships in the
Csabagyöngye-Bronnerstraube-Muscat ottonel (Ottonel muskotály) and the Királyleányka-Kövérszőlő combinations. -
Phylogenetic studies of Phoma species by maximum likelihood analysis
37-46Views:104The cosmopolitan Phoma genus contains mainly phytopathogenic, opportunistic parasite, and saprophyte fungal species. Up to now the characterization of Phoma species and other taxa of Phoma has so far been determined on the basis of morphology on standardized media, and gene sequence analysis was only used as a confirmative or distinctive complement.
In this study we have tried to study phylogenetic relationships by maximum likelihood method in the Phoma genus. We employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1) containing both introns and exons, a part of the gene responsible for synthesis of tubulin protein and ITS region containing the internal transcribed spacer regions 1 and 2 and the 5.8S rDNA as potential genetic markers to infer phylogenetic relationships among different Phoma taxa. Twenty-four isolates of eleven different Phoma species were firstly characterised by morphologically, and then their tef1, tubulin and ITS sequences were sequenced and analysed by maximum likelihood method carried out by PAUP*4.0b program. According to constructed phylogenetic trees, the different Phoma taxons are well separated. However these trees do not support the traditional Phoma sections based on morphological characterization.
The maximum likelihood analyses of all three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The experienced molecular evidences initiate the demand of reclassification of formerly mentioned soybean pathogens. -
Problem analysis of health tourism sector
27-31Views:115On the basis of experts health tourism is one of the most dynamically developing sectors within tourism. The outstanding role of the sector is partly owing to increasing health consciousness and partly to aging society – beside numerous other factors. But health tourism in Hungary is hit by several problems in spite of the fact that recently particular attention is paid to this sector. It can be mentioned as a difficulty that supply of education system is not in conformity with market demands, thus fresh supply of experts is not appropriate. As a world trend, classic position of thermal baths based on natural curative factors is getting to be displaced by adventure baths, which is unfavourable for health tourism. Notwithstanding that Hungary is in a distinguished place on a world scale as regards tourist arrivals, yet we are only in a mid-filed position relating to industrial revenues. Suppliers of this sector do not pay enough attention to disabled persons. The established TDM Organisations have to cope with numerous challenges. Nevertheless experts agree that high support of health tourism can be suggested despite to problems, market risks and significant international competition. However, this support has to be more aimed than before and has to be rather founded on world trends.