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Utility of Winter Wheat According to Visual and Microbiological Fusarium Infection, as Well as Toxin Examination
26-34Views:95In 1998 the Fusarium infection was studied visually and microbiologically and also F-2, T-2, HT-2, DAS and DON contamination were measured using 22 winter wheat samples. The correlation between the different parameters of 22 wheat samples were determined by regression analysis. According to our results we can state the following.
There is no significant connection between the results of visual, microbiological and toxicological examinations. This means that no certain conclusion can be drawn about the toxin contamination of samples – which is a determining factor of its utility – based on the visual symptoms and the plate dilution method.
Our results indicate however – though it is not proven statistically – that those samples in which the Fusarium infection did not exceed the limit of the standard, also had low toxin contamination, therefore they can be used as components of forage.
It is a considerable problem, however, that according to the visual qualification, such samples are excluded from the later utilisation, wherein the toxin contamination does not justify such action. Therefore, it is necessary to examine the toxin content of those samples which show high infection by visual symptoms. To reducing the number of expensive toxin examinations it would be advisable to change the currently used 0,5% limit which is indicated in the standard for a higher value of infection, for example to 2%, as recommended by Mesterházy (1998). -
Effect of cadmium and zinc contamination on the population dynamics of soil microorgani
73-77Views:111Changes in the population dynamics of microorganisms in a soil artificially contaminated with various doses of cadmium and zinc was examined from a quantitative point of view, under laboratory circumstances. The research was based on a chernozem soil originating from the area of a long-term microelement contamination model experiment (Nagyhörcsökpuszta, Hungary), which was carried out during 1991 in the Experimental Site of the Institute of Soil Science and Agricultural Chemistry, Centre for Agricultural Researche Hungarian Academy of Sciences, Budapest, Hungary. According to the amount of bacteria, microscopic fungi and nitrifying bacteria, it can be stated that the effect of contamination can be observed even in the perspective of nearly two decades. In more cases significant changes in the number of soil bacteria and microscopic fungi could be observed, and the nitrification activity increased in case of both microelements. Therefore the further research of changes in microbial activity of these soils can provide novel scientific results.
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Aflatoxin production on agricultural products
67-71Views:147Aflatoxins due to their toxicity pose significant economic and human health threat; therefore, it is important to avoid this type of contamination in agricultural products. Until now significant aflatoxin contamination occurred mainly in foods of tropical and subtropical origin because the optimal growth of the producer Aspergillus species is between 32–38 ºC. Nowadays the aflatoxin contamination is becoming higher threat in Hungary, due to the imported products, the rising average temperature and the climatic changes. There is a significant knowledge on the genetic and environmental effectors of the aflatoxin production; however, it is remained a great problem to control mold contamination and toxin production in farming and stock-raising. Here we attempted to summarize the knowledge on aflatoxin production and attempts of the elimination.
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The importance of selenium in the living world and in the scientific research
278-286Views:180Presently, selenium (Se) is one of the most investigated microelements. It has an important proven role in many vital processes. Directly or indirectly, selenium deficiency can play a role in the development of many diseases. On the other hand, the concentration range in which selenium is essential is narrow; there is a narrow gap between necessary and toxic content in dietary intake. In this context, selenium contamination poses a further health risk for people if they live near the industrial areas and mining activity.
In this paper, we comprehensively introduce the very important trace element selenium. We studied the base parameters, deposit, analytic and deficiencies, problem of contamination and also the solution of contamination problems of selenium. -
Surface polishing method application to reduce micotoxin content of mill wheat
339-342Views:141The fungi causing the infection and most of the harmful toxins they produce are concentrated in the bran of the grain, thus the intensive surface cleaning, the so-called debranning operation could allow the reduction of contamination in the milling technology. The essence of the PeriTec technology – originally developed by SATAKE, a Japanese company, to clean rice – is that it gradually removes the bran layers of the grain by mechanical means before further processing. We modeled the PeriTec technology with a laboratory size, batch-operating, horizontal debranning machine by SATAKE. The flour, milled grain after grinding 40 sec, the initial toxin content was only a small proportion (~15–20%) measured. The results showed that below the limit of DON toxin contaminated wheat (DON: 1.15 mg kg-1) during the grinding surface of the detached bran toxin contamination shows a very high (6.16 mg kg-1). The 40 seconds debranning before grinding shows lower DON toxin content than without debranning. So it is importance before the grinding. The toxin contamination of the bran fractions is significantly reduced, which is importance to the feeding point. As a result of debranning, the toxin content of the grinding fractions decreased, which justifies that that PeriTec method is suitable for the reduction of toxin contamination.
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Microbiological quality of bulk tank raw milk from two dairy farms in Hajdú-Bihar County, Hungary
105-112Views:303Two main channels have been identified to be responsible for microbiological contamination of raw milk and milk products. Firstly, contamination has occurred due to udder infection from the cow or the blood which harbours most bacteria that come in contact with the raw milk. Secondly, via external factors (may include faeces, skin, contaminated water, environment etc.) which are associated with the operation of milking. There is direct contact with the milk and/or surfaces before, during or after the milking, posing public health risk and economic decline. The aim of this study was to examine the bacteriological quality of bulk tank raw milk samples collected from two different size dairy farms (Farm 1 and Farm 2) of different housing forms (cubicle loose and deep litter) in Hajdú-Bihar County, Hungary in July, 2017. Three samples were taken from each farm, and the total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, and yeast and mould count were determined in them.
The results clearly showed low level of all measured bacteria group load in Farm 1 samples in comparison to Farm 2 with the exception of coagulase-negative Staphylococcus (CNS) which represented high level in general, indicating significant difference (P<0.05). The mean value of total plate count in Farm 2 samples was higher (1.0 × 105 CFU/mL) than Farm 1 samples (2.8 × 104 CFU/mL). There was a significant difference (P<0.05) in mean count of coliforms in raw milk samples between Farm 1 and Farm 2. Similarly, results of E. coli were significantly different (P<0.05) with mean count of 1.44 × 102 CFU/mL and 2.02 × 103 CFU/mL for Farm 1 and Farm 2 respectively.
Results of Staphylococcus aureus also showed significant difference (P<0.05) with mean count of 9.7 × 101 CFU/mL for Farm 1 and 6.28 × 102 CFU/mL for Farm 2. The mean of mould count recorded was 1.07 × 102 CFU/mL and 4.93 × 102 CFU/mL for Farm 1 and Farm 2 respectively. The recorded mean of yeast count was 1.68 × 103 CFU/mL and 3.41 × 103 CFU/mL for Farm 1 and Farm 2 respectively; however, both farms showed no significant difference (P>0.05) in terms of mean of mould and yeast count. Although Farm 2 produced six times lower milk quantity than Farm 1, the measured microbial parameters were high. Both farms’ microbiological numbers were higher above the permitted limit values as stated by Regulation (EC) No 853/2004, Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.).
This could be an indication of non-conformance to effective GMP, ineffective pre–milking disinfection or udder preparation, poor handling and storage practice, time and temperature abuse and inadequate Food Safety Management System Implementation. Therefore, our recommendation is as follows; establish control measures for pre- and postharvest activities involved in the milking process which would be an effective approach to reduce contamination of the raw milk by pathogenic microorganisms from these farms, strict sanitation regime and hygiene protocol be employed and applied to cows, all equipment, contact surfaces and minimize handling of the milk prior, during and after milking. This will also serve as scientific information to the producers for continual improvement in their operations.
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Heavy Metals in Agricultural Soils
85-89Views:72The soil constitutes the basis of the food chain. To keep soil conditions in a good trim is very important, it’s part of the sustainable development and of producing food supply harmless to health.
In some cases, soil productivity is the only important part, qualitative requirements or economical characteristics can improve it. The soil is threatened by two danger factors: the soil degradation and the soil pollution. The accumulation of different harmful and/or toxic substances in the soil is well known. Heavy metals constitute a part of it. Metals in the soil and in the soil-solution are balanced. This balance depends on the type of the metal, on the pH, on the cation-band capacity of the soil, on the redox relations and the concentration of cations in the soil.
To be able to handle the metal contamination of the soil, it is important to estimate the form, the possible extension and the concentration of metals.
Of course, the different types of soils have different physical-chemical, biological and buffer capacity, they can moderate or reinforce the harmful effects of heavy metals. To draw general conclusion of the dispersion and quantitative relations on the metals originated from different contamination sources is hard, because in some emissive sources contamination is limited in small areas but on a high level, some others usually expand on larger areas, and as a result of equal dispersion, the contamination’s level is lower.
Heavy metals – unlike alkali ions – strongly bond to organic materials, or infiltrate in a kelát form. Their outstanding characteristic is the tendency to create metal-complex forms. Kelats take part in the uptaking and transportation of heavy metals. Heavy metals exert their effects mostly as enzyme-activators.
The metals cannot degrade in an organic way, they accumulate in living organisms, and they can form toxic compounds through biochemical reactions.
Lot of the heavy metals accumulate on the boundaries of the abiotic systems (air/soil, water/sediment), when physical or chemical parameters change, and this influences their remobilization.
Human activity plays a great part in heavy metal mobilization, results in the human origin of most biochemical process of metals.
To understand the toxic influence of accumulated metals of high concentration, their transportation from soils to plants or their damage in human health, must clearly defined and investigated.
For effective protection against soil pollution, the types and levels of harmful pollution to soil must identified, regarding legal, technical and soil-science aspects, preferable in a single way. Difficulties in this area mean that toxicity depends on loading, uptake, soil characteristics and living organisms (species, age, condition etc.), furthermore, local and economic conditions considerably differ. -
Wheat cleaning and milling technologies to reduce DON toxin contamination
89-95Views:210Mycotoxicosis caused by Fusarium fungi holds a huge risk considering economic and food safety issues worldwide. By applying milling technologies, we attempted to reduce the concentrates of DON toxin, as it is the most often found toxin in wheat.
The processes of sieving, aspiration and combination had been used on wheat with high DON toxin concentration. As a next step, grains were sorted using a horizontal cylinder separator, assorted by an optical and a gravity separator, and finally, the products were scoured and ground. The contamination level of the wheat and flour samples were defined by the HPLC-MS method.
Regarding the results, it can be stated that toxin concentration was most effectively reduced by optical separation and scouring, and by applying these milling techniques, food safety can be increased significantly.
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The effect of keeping technology on the microbiological status of raw milk
67-75Views:114The importance of the quality of raw milk increased after Hungary had joined to the EU. On delivery of raw milk, the microbiological quality, especially total plate count of the milk is very important. Twenty-two farms (7 large, 4 medium-sized, and 11 small farms) were included in the study. We considered the different farm size, keeping- and milking circumstances during the selection of farms. The examined large farms use loose housing system (cubicle, deep litter) and milking parlour. Most of them use preand post-milking disinfection. In the medium-sized farms, loose,
deep litter and tie-stall housing system, as well as milking parlour, pipeline milking and bucket milking occurred. All of them use preand post-milking disinfection. Small farms use tie-stall housing system, bucket milking and udder preparation by water. Unfortunately, they do not use pre- or post-milking disinfection. In the large and medium-sized farms mainly Holstein Friesian, in the small farms Hungarian Simmental breeds can be found.
The aim of our research was to examine the microbiological status of the raw milk produced in dairy farms (total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, psychrotroph bacteria count, furthermore yeast and mold count); sources of the contamination; connection between the microbiological quality of produced milk and housing-, milking technologies of farms; furthermore the hygienic circumstances of milking and milk handling of the farms, by the examination of coliform bacteria and Escherichia coli contamination.
During the examination of the connection between the different farm sizes, various housing- and milking forms and the microbiological characteristics we observed similar tendencies in the case of total plate count, coliform count, yeast and molds count, furthermore psychrotroph bacteria count. The value of these parameters was significantly higher in small farms, and infarms which use tie-stall housing forms, bucket milking, udder preparation with water, and which do not use pre- and post-milking disinfection.
The results showed that besides cooling, the milking procedure and the type of udder preparation had the largest effect on the total plate count. Statistical analysis shows that in medium and small farms the combination of pipeline milking – tie stall housing system – disinfectant preparation of the udder; in large farms the combination of milking parlour – loose cubicle housing system – dry preparation of the udder are the most appropriate in the aspect of the total plate count. We experienced that in farms where the hygienic instructions are not followed – and therefore
equipment used during the milking and handling of milk is very contaminated – or rather the separation of mastitic cows’ milk is not appropriate, different microorganisms may contaminate the produced milk. -
The study of headspace sampling for the measurement of Volatile Organic Compounds byGC-MS
35-38Views:126Headspace sampling was studied for GC-MS measurements. A quick, direct method was developed for the measurement of petrol, gasoline and volatile organic solvent contamination of soil. According to our results this method is suitable for quick qualitative analysis of VOC contamination of soil, and helps to make decisions about further measurements. We concluded that an accurate quantitative measurement is not possible.
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Germination and sugar content alteration in maize grain caused by Fusarium contamination
42-44Views:67Healthy and Fusarium affected ears were collected in Fészerlak, Somogy County at the end of vegetation cycle of maize (25-30 August). Each pattern contains 25-25 ears. We compared the samples on the basis of visual image of Fusarium affection. The ears were shelled and two 0,5 kg samples were formed: healthy and Fusarium contaminated. After surface sterilisation the uniform sized seeds were soaked in sterile distilled water for 24 hours and there were germinated for 7 days. The α-amylase activity was measured with Phadebas- α-amylase test. Seeds were extracted one by one three times under reflux using 10 cm3 boiling water for 15 minutes. During our investigation germinating activity was detected to measure glucose, fructose, sucrose content and α-amylase activity. In the first seven days of germination the highest values were detected in control seeds followed by the affected seeds. Our results clearly show that stress conditions applied altered not only the saccharide content but decreased their germinating activity as well in the case of maize grain.
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A tárolási feltételek hatása a kukorica Fusarium fertőzöttségére és toxin szennyezettségére
28-32Views:221Corn samples harvested in 1997, 1998 and 1999 from different soil types were stored at different conditions (temperature, moisture content, state of kernels) for six months. The Fusarium contamination was examined by plate dilution method and the amount of mycotoxins (F-2, T-2, HT-2, DON, DAS) were determined applying HPLC method immediately after harvesting and in the third and sixth month of storage. The aim of our study was to find correlation between the ecological factors, storage conditions and the examined parameters mentioned above, as well as to prove them statistically. According to the examinations carried out after harvesting we could state that the soil type had no effect on the parameters. Analysing the effect of the years we found considerable differences. The Fusarium infection of corn samples in 1998, while the toxin contamination in 1999 was the highest. The results of storage experiment show that year (number of microscopic fungi, F-2, T-2, HT-2, DAS and total toxin content) and moisture content of kernels (F-2, T-2, and total toxin content) have a significant effect on the examined parameters. We could prove the effect of temperature on the T-2 content (samples with natural moisture content) and DON content (samples with 14% moisture content). Higher values were found at higher temperature storage. The ratio of damaged kernels influenced the DON concentration in the non- wetted samples and the number of microscopic fungi in the wetted samples.
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Mycotoxin contamination in maize triggered by arthropod pests and the related protection possibilities
59-64Views:143Mycotoxin contamination in harvested maize has increased in the last decades, which can be unequivocally back to the plant health troubles caused by global warming. The increasing of wounds in maize crops was occurred by climate change both on direct (hailstorm) and indirect
(newly appeared pests) ways. In additional, the settling phytopathogenic microfungi on these plant wounds inflict serious human and animal health problems.
The changing of Hungarian arthropod pests assemblages stand in the background of this dangerous nuisance complex. The spreading of European corn borer (Ostrinia nubilalis Hbn.) bivoltine ecotype as well as the newly appeared adventive species [cotton bollworm Helicoverpa armigera Hbn.), western corn rootworm (Diabrotica v. virgifera LeConte), fourspotted-sapbeetle (Glischrochilus quadrisignatus Say)] in Hungary can be responsible for this situation. In total, all technological elements, which obstruct the damage of these chewing mouthparts pests, as well as moderate the mechanical damage of maize, can be contribute to the reduction of both these phytopathogens injuries and mycotoxin contaminations. -
The roles of mycotoxins in cereal crops production: A comparative study of Hungary and Tanzania
151-159Views:188Although Hungary and Tanzania's climatic, soil, and technological conditions differ significantly in crop production, cereals crop cultivation is of paramount importance; maize crop dominates the cultivated area (Hungary 1 million ha, Tanzania 3 million ha) both from a feed and food point of view. Unfortunately, in both countries, fungal species (Fusaria, Aspergilli, Penicillia, etc.) that produce various mycotoxins on cereals, including maize grains, are a growing concern. The situation is complicated because these fungal species and their toxins can appear not only on cereals but also on other crops. Despite the prevalence of mycotoxins in both countries, studies show higher exposure risks and contamination above tolerable levels for human consumption in Tanzania to Hungary, with Tanzania observing acute aflatoxicosis.
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Species-specificity of the glutathione reduced form accumulation in the arboreal plants, testing influence of industrial emissions
105-110Views:72Conducted are researches of dynamics of glutathione reduced form maintenance in the leaves of arboreal plants which grow in the zones of different level of contamination by industrial emissions of“Red Lead Factory” LTD and ArselorMittal Kryvyi Rig. For comparison, similar investigations were conducted in the intact Botanic Garden in s.c.t. Sofievka. Findings testify to absence of negative influence of industrial emissions and, thus, the arboretum of botanical garden also can be examined as conditional control. It was stated that it is set a few strategies of active oxygen forms and their metabolites deactivation, appearing in cells under action of environment negative factors. To our opinion, fluctuation of glutathione reduced form maintenance can examine as an index of plants stability, on which investigational species can be distributed on steady and moderato steady.
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Activity of some enzymes, participating in nitrogen compounds transformation in chernozem, polluted by fluorine compounds
99-104Views:96Contamination of chernozem by fluorine compounds variously affects those enzymes (urease, asparaginase, glutaminase, arginase, amidase), which takes part in the metabolism of nitrogen-bearing organic compounds. In broken soils the inhibited desaminisations is stronger, than enzymatic hydrolysis of asparagine and arginine. The features of seasonal dynamics of change activity of urease and correlation dependence of its activity from some physical and chemical soils properties are described. These tendencies well comport with the results of model experiments. At minimum HF influence there is inhibition of processes of monohydrocarboxylic acids desaminisation, hydrolytic breaking up of arginine and glutamine. By a side with this there is activating of urea and asparagine breaking up processes on the initial stages of toxicant influence. The study of kinetics of process of urea enzymatic hydrolysis in chernozem at the different level of HF influence showed changes of initial and maximal velocity of enzymatic reaction, and also Michaelis-Menten constant.
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Coagulase-negative staphylococci in ewe udder surface and raw milk samples
221-225Views:157Coagulase-negative staphylococci (CNS) are among the major causes of subclinical mastitis in dairy ewe flocks. This has a financially significant impact on the ewe dairy sector and consumer health. The determination of the bacterial count, particularly CNS, is critical in terms of the quantity and quality of ovine milk. Thus, the purpose of this study was to quantify the CNS count in the udder surface and raw milk samples of the ewe, in addition to identifying CNS strains (n=8) collected from udder surface and individual raw milk samples by 16S rRNA gene sequencing. A total of 164 samples of udder surface and raw milk originated from four sheep farms were tested. The obtained values were compared between the different farms. Furthermore, values during 2018 and 2019 in the case of Farm I were compared. Significantly higher (p<0.05) average count was observed in udder surface samples taken from Farm I (2.8±1.0 lg CFU/cm2) than that of Farm III samples (2.3±0.6 lg CFU/cm2). In the case of individual raw milk, the higher value was observed in samples derived from Farm III (3.5±0.9 lg CFU/mL), while the obtained value was significantly lower (p<0.05) in samples originated from Farm IV (1.8±0.4 lg CFU/mL). In the bulk tank milk samples, the highest mean value was 5.3±0.4 CFU/mL, and there was no significant (p>0.05) variation between farms. Coagulase-negative staphylococci counts were decreased to a certain extent in both sample types tested during 2019 except for individual raw milk derived from the Tsigai breed. The correlation between the mean CNS counts of udder surface and individual raw milk was very weak (r=0.048). Staphylococcus simulans, Staphylococcus auricularis, and Staphylococcus equorum were identified by molecular sequencing and Staphylococcus simulans were the most frequently identified CNS species. A higher CNS count of bulk tank milk than individual raw milk indicates possible contamination during milking and storage. Therefore, further studies are required to investigate the other sources of bulk tank milk contamination to improve the hygienic quality of milk.
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Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:128Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested. -
Opportunities for improving the nutritional value of cereal-based products
275-278Views:221We set up experiments for improving the nutritional value of cerealbased products. Our aims were to decrease the energy content of bakery products with the mixing of plant originated raw materials and byproducts with high fibre contents, and we have evaluated the effect of sodium-chloride on the physical properties of bakery products. We found that the apple pomace, the byproduct of juice production, is excellent for increasing of fibre content of bakery products and the further aim of investigation is to develop economical ways of hygienic byproduct handling and purification. Our experiments, evaluate the bakery use of triticale, have significant achievements and the breads made from triticale flour and whole-grain are commercially available nowadays. Our investigations included the possibilities of decrease of toxin contamination of cereals and our results can form a part of risk estimation systems after further experiments .
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Intensity of free radical processes in the leaves of arboreal plants under act of industrial dust borne extracts
83-87Views:65The influence of industrial pollutants on the intensity of lipid peroxidation in the assimilatory organs of arboreal plant was investigated. The differential changes of the probed indexes are set depending on the species. Information is got can testify to participation of lipid peroxidation products in forming of reactions-answers of arboreal plants on influence of industrial dust borne extract with content of heavy metals. Determination of level and rates of accumulation of Zn, Ni, Pb and Cd, in the leaves of arboreal plants in the conditions of different
contamination level allowed to take species to two groups. To the first (phytoextraction potential exceeds a base-line level in 10 times) belong Populus bolleana Lauche, P. italica (Du Roi) Moench, Picea pungens Engelm and Sorbus aucuparia L. To the second (exceeds a base-line level from 5 to 10 times) belong Acer negundo L., Aesculus hippocastanum L., Betula pendula Roth and Tilia cordata Mill. The most substantial increase of peroxidation secondary product content (more than in 2.5 times) is peculiar for B. pendula, A. hippocastanum and P. pungens Engelm., that well conforms to the rates of heavy metals translocation, it has however species-specific character. -
Evaluation of the blood product characteristics of meat meal and hemoglobin with non-invasive methods in the VISNIR wavelength
49-56Views:243The separate collection of poultry slaughterhouse trimmings and blood is partially solved in Hungary. Only properly prepared animal by-products, protein meals can be utilized as animal feed additive. However, different protein meals are appropriate for feeding different animal species. That is the reason why it is important to avoid accidental cross contamination of the products. Meat and blood meal produced on the same technological line, therefore mixing of the products can happen in various proportions during the shift of production.
Thus the aim of this study is to develop a spectral method which will allow to estimate the ratio of meat and blood protein meal in the final product. During the test the products were mixed in different proportions and were examined by the spectral method. Measurements were conducted with AvaSpec 2048 spectrometer in visible (VIS) and in near infrared (NIR) wavelength range (400–1000 nm) to define the spectral differentiation of the different meal products. Significant difference can be detected in spectral reflectance between the meat and blood product in the VIS-NIR range. The blood product has a characteristic spectral property: in the range of 600 and 735 nm reflectance values are increasing following a sigmoid curve. This property is not observed in the case of meat meal: close to linear rising is detected. Effective protein rate and purity detection could be made by Blood Product Sensitive Mixing Index (BPSMI – R930/R600), and by the calculation of inflection point in 600–735 nm.
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Some aspects of food safety from animal origin
153-155Views:118The relationship between the civilisation illnesses and incorrect nutrition can be considered as demonstrated. The functional foods having one or several nutritional- biological advantage can contribute to the healthy nutrition and to improving the quality of life. Beside the healthy nutrition it is an another important requirement that the food does not contain pathogens or substances of chemical and biological origin namely it has to be safe.
In this publication the foods of animal origin were investigated in the point of view of public health hazards arising during their production and processing.
The contamination and infection of food raw materials, the consequence of chemical substances getting in or developing it the food, the judgement of food deviring from genetically modified or cloned animals are discussed. -
Environmental Consequences of Efficient Use of Nitrogen Fertilizers
41-46Views:78Nitrogen fertilizer represents major economic burden. For this reason, although the efficiency of nitrogen utilization varies highly, its actual use generally remains at low levels; these averaging between 25 and 50%. We set up an experiment at the Oradea Research Station, using 15N labeled fertilizers, in order to investigate the possibility of increasing N fertilizer efficiency in winter wheat under irrigation conditions.
Fertilizers labeled with 15N allows us to individually determine its effect on yield formation, as well as the use efficiency of N from fertilizer following application rate and time. The amount of N derived from fertilizer as determined in straw and grain yield is high. When the labeled fertilizer is applied at tillering time, the values of this indicator rise when higher N levels we applied.
In separate experiments, we investigated a series of aspects connected to chemical fertilizer regarding the determination of the type of fertilizer, optimum time and rates of application; all these as a function of the special pedoclimatic conditions.
The results obtained in the field show that the effectiveness of N utilization in wheat is most variable and generally low, often ranging between 25 and 33%, owing to N loss within the system through leaching and NH3 volatilization.
A readily achievable increase in efficiency of 5 percentage points would result in considerable savings, and can be brought about by reducing nitrogen losses. The added benefits to the environment in terms of reduced ground/water contamination and lowered nitrous oxide (N20) emissions would also be substantial.
The figures for N fertilizer use efficiency (% N range from 35.5 to 72.6, the highest value being recorded with an N application of 120 kg/ha at tillering, when the previous crop was sunflower).
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Applicability of hyperspectral technology for in situ phytoremediation
71-78Views:111The characterization of heavy metal polluted abandoned mining sites is a complicated assignment due to the variable spatial distribution of the pollutants, therefore complex integrated method is required in order to assess precisely the amount and the distribution of the contaminants. The examined area is flotation sludge reservoir of abandoned Pb-Zn mining site with serious heavy metal contamination. located in Gyöngyösoroszi, Northern Hungary.
The hyperspectral image of the flotation sludge is obtained by using a Digital Airborne Imaging Spectrometer DAIS 7915, in the frame of DLR HySens first Hungarian hyperspectral flight campaign (21/08/2002). Parallel to the flight campaign heavy metal content of soil samples were examined from the area of the flotation sludge. The analysis of hyperspectral data was verified by the examination of mine tailing samples by FPXRF (Field Portable X-ray Fluorescence spectrometry) (NITON XL-703).
Determinations of heavy metal containing minerals are based on the spectral profiles of the pixels of the area with using USGIS standard spectral profiles of the examined materials (galena, pyrite, sphalerite, goethite and jarosit).Applying the Spectral Angle Mapper with BandMax classification the distribution of minerals (galena, pyrite, sphalerite, goethite, jarosit) in the area was defined. The mineral formation occurs especially at the levees and the barren places of the Szárazvölgyi flotation sludge reservoir. Based on the statistic results of the samples, principal component analysis and correlation coefficient between the different metal content of the samples were calculated. The highest correlations were found between Pb-Zn, Fe-Zn and between Fe-Pb. This prove the results of the principal component analysis, where usually Pb, Zn, Fe introduce the main component.
Canopy analysis was also carried out with the hyperspectal image in order to classify the differences between vegetation types at the Szárazvölgy flotation sludge reservoir and analyse the applicability of it. Supervised classification methods were used to distinguish 8 vegetation types based on the spectral properties of the area. The results of the classifications were compared to a ground truth image, based on ortophoto, topographic map, and GPS based field data collection. According to results of the comparison, the paralellpiped classification method is proved to be appropriate method based on the overall accuracy of canopy classification, which was 54% due to heterogeneity of the vegetation.
The results of hyperspectral data and FPXRF analysis suggest that Pb, Zn and Fe containing minerals have similar spatial distribution in the examined and barren area.
Based on this study hyperspectral remote sensing is likely to be an effective tool for the characterization and modeling the distribution of Pb, Zn and Fe containing minerals at the examined heavy metal polluted sites. Further more, based on the vegetation analysis plant species for phytoremediation can be defined.
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Correlations of the global, regional and local factors of the anthropogenic effects on the water reserves of the Earth’s crust
85-92Views:102The decrease in Earth's drinking water resources and the degradation of its quality has become a critical problem. Our planet's total water supply is estimated to be around 2 billion km3s. This is only 1% of Earth's own weight. Of this small amount only a tiny 3% is fresh water, of which 79% is forzen in glaciers and 1% is present as surface waters.The ratio of water stored in soil is around 20%. This is 0.2% of the total water supply. Our study aims to summarize the layered groundwater aquifer systems and its changes which are the results of anthropogenic effects in both global and hungarian respects and also for the region of Debrecen.In particular with regard to the geological and ecological level where irreversible
processes take place. All this is discussed in the context of cause and effect. Pointing out the dangers of excessive deep groundwater extraction and the contamination caused by toxic substances that are the byproducts of modern life. In addition we discuss the Water Directive of the European Union which gives a policy for community action concerning the goal to achive the status of „in good condition” for our waters till 2015.