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The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:163he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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Effect of cadmium and zinc contamination on the population dynamics of soil microorgani
73-77Views:122Changes in the population dynamics of microorganisms in a soil artificially contaminated with various doses of cadmium and zinc was examined from a quantitative point of view, under laboratory circumstances. The research was based on a chernozem soil originating from the area of a long-term microelement contamination model experiment (Nagyhörcsökpuszta, Hungary), which was carried out during 1991 in the Experimental Site of the Institute of Soil Science and Agricultural Chemistry, Centre for Agricultural Researche Hungarian Academy of Sciences, Budapest, Hungary. According to the amount of bacteria, microscopic fungi and nitrifying bacteria, it can be stated that the effect of contamination can be observed even in the perspective of nearly two decades. In more cases significant changes in the number of soil bacteria and microscopic fungi could be observed, and the nitrification activity increased in case of both microelements. Therefore the further research of changes in microbial activity of these soils can provide novel scientific results.
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Optimization of inductively coupled plasma mass spectrometer parameter’s to measuring arsenic and selenium
59-64Views:215In the last decades an increased interest has been evolved about arsenic and selenium. The aim is to understand the environmental, agricultural and biological role of the these elements. In case of arsenic the mayor reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking waterbases of some Asian countries besides Hungary. The toxicity of higher level selenium content is also known, nevertheless selenium is essential for some biological functions. Considering its esssentiality, in our country the insufficient selenium intake rate couse lack of selenium. Measuring the concentrations of these elements are cruital but not satisfactory information, but the speciation, that is the form of an element presented in a sample is also required.
In both cases the most suitable method to determine concentration is the inductively coupled plasma mass spectrimetry. My objective was to optimase the changeable parameters of the ICP-MS for reaching the lowest (the best) detection limit. For this porpuse I have investigated the effect of parameter change on nett signal intensity and relative signal intensity. With the optimased parameter settings the limit of detection for arsenic and selenium were determined, which are 0,032 ng cm-3 for arsenic, and 0,097 ng cm-3 for
selenium. -
Parameter optimization of an inductively coupled plasma mass spectrometer for measuring arsenic and selenium
81-85Views:175In the last decades, an increased interest has evolved in arsenic and selenium. The aim is to understand the environmental, agricultural and biological roles of these elements. In the case of arsenic, the major reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking water bases of some Asian countries, as well as Hungary. The toxicity of higher level selenium content is also known; nevertheless, selenium is essential for several biological functions. Considering its essentialness, in our country, the insufficient selenium intake rate causes a lack of selenium. Measuring the concentrations of these elements provides crucial, but unsatisfactory information, as the speciation, i.e. the form of an element presented in a sample is also required.
In both cases, the most suitable method to determine concentration is inductively coupled plasma mass spectrometry (ICP-MS). Our objective was to optimize the variable parameters of the ICP-MS to attain the lowest (the best) detection limit. For this purpose, we investigated the effect of parameter change on net signal intensity and relative signal intensity. With the optimized parameter settings, the limits of detection for arsenic and selenium were determined, which are 0,032 ng dm-3 for arsenic, and 0,097 ng dm-3 for selenium.