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The effect of hybrid, nutrient-supply and irrigation on the grain moisture content at harvest and the starch-content of maize (Zea mays L.)
89-95Views:229Maize is a worldwide dominant plant. According to nowadays plant production principles it is important to investigate and optimize the site-specific nutrient-supply and other production factors, such as hybrid and irrigation, in the case of this plant as well.
At the Research Institute of the University of Debrecen, Center of Agricultural Sciences and Engineering, at Látókép the effect of nutrient-supply and irrigation on the quantity and quality parameters of different hybrids were investigated in a small plot long-term field experiment. In this paper we introduce the results regarding the corn moisture-content and the starch content of the yield.
We have chosen three maize hybrids – that have been bread in Martonvásár – for our investigations. The effect of macronutrients is investigated in this experiment on five levels. The half of the experimental area can be irrigated during the vegetation period – whenever it is needed – by linear irrigation equipment, but on the other half only the water amount originating from the precipitation can be used by plants.
In the year 2008 the hybrid affected the grain moisture content at P=0.1% level, while nutrient-supply had an effect at P=10% significance level. We haven’t revealed either any effect of irrigation or of interrelationship between production factors. It can be stated that there are differences between the hybrids on each nutrient-supply and on both irrigation levels. The grain moisture content increased parallel to the longer vegetation
periods.
The starch content of maize is mostly affected by the hybrid,
so on P=0.1% significance level. Regarding our results, it can be
stated, that the starch content shows a decreasing tendency
parallel to the longer vegetation periods. -
Mitigation of environment impact of mycotoxins produced by Fusarium species
159-164Views:501The Fusarium fungi hazards the grain quality of cereals, therefore significantly affects their utilization as animal feed or consumable product. The Fusarium can decrease the quality of wheat in different ways: decreases the germination capability, causes visible discoloration, mould may appear, reduces the dry material and nutrient content of the grain, causes mycotoxin infection – as a result given by its by-product. Micotoxins produced by Fusarium genus, as the trichotecenes (T-2, HT-2, deoxynivalenol, nivalenol, diacetoxyscirpenol, Fusarenone-X) and the zearalenone (F-2) are the most common in Hungary. Occurrence of fumonisins first discovered in 1988 are must be identified carefully. About 20–30% of the overall worldwide production of cereals is infected with Fusarium and its toxins, which situation is similar in Hungary. This infection causes serious yield-losses in cereal production. In the case of cereal products, which non-utilizable as forage seems, an optimal solution is utilizing as biogas raw material, but it is also important to examine the effect of the infected cereal on the anaerobe digestion process.
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Evaluation of automated anaerobic fermentation processes as in the case of mould infected maize
81-86Views:523In Hungary the renewable energy utilization is planned to achieve 13% by 2020. Biogas production is one of the fields with the largest energy potential. Achieving high efficiency during continuous production despite the mixed and variable composition of input materials is the most common problem which the newly built biogas plants using agricultural raw materials have to deal with. The first experimental reactors at the Department of Water and Environmental Management were built 12 years ago. Control and automation of the four separated bioreactors were executed with ADVANTECH GENIE 3.0 software which granted pre-programmed measurement and points of intervention for pH, temperature, CH4, CO2, H2S, and NH3. The system became out-of-data, therefore in 2010 it has been redesigned and tested. The system is controlled by Compair Proview SCADA (Supervisory Control and Data Acquisition) software running on Linux platforms. The Fusarium infection caused serious yield-losses in cereal production in 2010. In the case of cereal products, which non-utilizable as forage seems an optimal solution is utilizing as biogas raw material. The raw material was based on the Fusarium infected maize. In the recent publication infotechnological and technological experiences of the pilot test period are evaluated as well as direction of future development is defined.
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Dimensionality Expressed by Caseendings and Spatial Prepositions
7-15Views:211The purpose of this essay is to investigate some of the uses of English prepositions and Hungarian case endings employed to express spatial relations. The observation of invariant mistakes Hungarian native speakers learning English make initiated the investigation. The questions raised are: (a) where do the two systems match and where do mismatches lie, (b) how do language users perceive the world, and (c) do speakers observe spatial relations as two-dimensional or three-dimensional cognitive models? Do different languages see the same thing as either three-dimensional, or two-dimensional?
Abondolo (1988) gives an adequate morphological analysis of ten Hungarian case-endings (inessive, illative, elative, superessive, delative, sublative adessive, ablative, allative and terminative) used in spatial reference, which give a closed set in references made to factors, such as (1) location which can be broken down as interior vs. exterior location with the latter being further analysable as superficial and proximal, and (2) orientation which can be analysed as zero orientation (position), source and goal. In addition to those in this list, two other case endings (genetive/dative and locative) are also used for expressing spatial relations but the last is only a variant of the inessive and superessive case-endings and is only used with place-names. The set is closed in the sense that the same item is meant to refer to the same sort of spatial relation in every case. Language textbooks, c.f. Benkő (1972) seem to suggest a neat match between the above Hungarian case endings and their English prepositional counterparts, e.g. London-ban (inessive) = in London.
The picture, however, is far from being so clear-cut. The data, which were taken from various dictionaries and textbooks, show that the choices of both the prepositions and the case endings listed above depend on how the speaker considers factors (1) and (2) and that proximity is very important. Instead of a one-to-one match between the prepositions and the case endings, we rather find that the above case endings will match a dual, and in some cases a tripartite system of prepositions with the correspondences found in the two languages, which yield the following chart:
We suggest that languages may view or map the same physical entities in different ways, for example along surface vs. volume or goal vs. passage, etc.
Furthermore, we also find it possible that it is the language specific, inherent coding of the nominal phrase that decides – in many cases – upon the choice of prepositions and case endings. -
Comparative analysis of maize weed control system and the competitive effect of sorghum
97-104Views:327In our investigation we used different weed control technologies in the different phenology states of the maize. The experiment have been
carried out in Hódmezővásárhely, in the Experiment garden of the Pilot farm of University of Szeged Faculty of Agriculture, on meadow
chernozem soil, on 24 m2 plots, in 3 replications, randomized blotch design. The experiment can be regarded as 15 weed-control strategies
where, in addition to the untreated control, six chemicals or chemical-combinations are applied (Spectrum 720 EC, Motivell Turbo D,
Stellar + Dash HC, Clio + Akris SE + Dash HC, Clio + Dash HC) in five different times (pre, early post, post and two late post) and eight
mechanical weed-control technologies were used. Hoeing took place connected to the herbicide treatments in different times: until 2-3-leave
age weedless, in 3-4-leave age hoed once, from 3-4-leave age weedless, in 6-7-leave age hoed once, from 6-7-leave age weedless, in 8-leave
age hoed once, from 8-leave age weedless.
Our results were assessed by chemical efficiency examination, maize length measurement, corncob-length and fertility examination,
Sorghum plant-number determination and yield weighing carried out in four periods. The data were evaluated by a one-factor analysis of
variance and a two-factor linear regression analysis. -
Different soil fertility conditions depending on different land use methods
169-172Views:204In precision nutrient management the most important aspect is adaptation but we should consider the possibility of the long-term improvement of soil fertility within the less fertile landscape zones. This possibility can be evaluated principally by long-term field experiments, which are running on similar soil types. The results of these field experiments can indicate that which soil fertility status should be attained. Some more important soil fertility data, (such as pH, P-, K- and soil organic matter (SOM) content) of a long-term field experiment with increasing farmyard manure(FYM) doses or equivalent NPK fertilizers, set up on an Eutric cambisol, are presented. The yieldincreasing capacity of FYM doses was only 82%, as compared to the equivalent amount of mineral NPK, but long-term FYM treatments resulted in 10% higher SOM content than that of equivalent NPK
fertilizer doses. The studies indicate that SOM content is a function of local climate and clay content of the soil, and neither long-term high FYM doses can increase SOM content steadily above a supposed steady-state value. However we have to make efforts to keep the optimum level. The lowest soil reactions developed both with the highest NPK doses and without any fertilization. AL-P2O5 content of soil was increased more by mineral fertilization than by FYM treatments, but in case of AL-K2O content there was no difference between the fertilization variants. However the highest doses of both fertilization variants increased soil nutrient content to an excessive degree. Wecould get very valuable data from the unfertilized control plots as well, where long-term yield data suppose 48 kg ha-1 year-1 air-borne N-input. -
The impact of sowing date, production area and nutrient supply on the yield and quality parameters of millet
105-109Views:244The millet is a very special plant with good adaptation that gives the possibility for the late sowing and secondary production. However the effects of late sowing modifies to the efficiency of the agrotechnological elements. The examinations – focused on this aspect – was conducted in the DU CAS RINY in the small plots in four replications in 2013. The largest effect was recorded in the sowing time of the examined factors (sowing time, nutrient supply, growing area). The effectiveness of the agrotechnological elements decreases under unfavourable circumstances caused by the late sowing. The examined factors did not affect in the protein content and milling rate, except in the latest sowing time when protein content was significantly the highest.
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Polymorphism of the bovine GH and LEP genes in a population of Slovak spotted bulls
19-23Views:282The aim of this study was the detection of polymorphism in the bovine growth hormone and leptin genes using the PCR-RFLP method. A
polymorphic site of the growth hormone gene (Alul loci) that results in amino acid change at position 127 of the protein chain (leucine, L to
valine, V) has been linked to differences in circulating metabolites, metabolic hormones and to milk yield. The polymorphism in bovine leptin
gene is situated in the intron between two exons, which results in an amino acid change at position 2059 of the protein chain (cytosine, C to
thymine, T). The polymorphisms were studied in a group of 58 bulls of the Slovak spotted breed. A strategy employing PCR was used to amplify 428 bp (GH gene) and 422 bp (LEP gene) products from blood samples. Digestion of PCR products with restriction enzymes AluI and Sau3AI revealed alleles: L and V; A and B for GH gene and LEP gene, respectively. The growth hormone gene is a candidate gene for body weight gain in cattle, since it plays a fundamental role in growth regulation. Leptin plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle; therefore, animals with higher leptin gene expression will probably have lower daily weight gain than others with similar forage offer and nutritional condition and will also likely have longer calving intervals. -
Analysis of longevity in Holstein Friesian cattle using proteomic approaches
21-25Views:631The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,
we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group. -
Contexts between apple orchards with various cultivar comparisons and the effect of ATS (ammoniumthiosulphate) on fruit thinning
35-38Views:271Our research focuses on a fruit thinning material that can also be used in apple production. This material reduces significantly the required manual labor of hand thinning by russeting the blossoms selectively. The ATS (ammonium-thiosulphate) acts as chemical desiccant contrary to the nowadays commonly used materials such as naphthalene acetic acid, naphthyl acetamide, benziladenin and ethylene, which affect the metabolic processes of the plant by regulating the hormone system. In our experimentals cultivar ’Pinova’ and ’Golden Reinders’ were treated with different concentrations of ATS. The effect of these doses on the fruit setting and the quality and quantity parameters of the fruits was studied. According to our results, in the case of cultivar ’Pinova’ the ATS did not have any detected effects at the concentration of 1.5%. Application of ATS at 3% decreased considerably the fruit setting and fruit yield, accordingly the mean fruit size improved. The response to treatment in the case cultivar ‘Golden Reinders’ does not have any similar consistent results.
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Impact of nitrogen and sulphur fertilization on the growth and micronutrient content of spring wheat (Triticum aestivum L.)
211-219Views:383Micronutrients are as important as macronutrients for crops. Each micronutrient has its own function in plant growth. Zinc is important for membrane integrity and phytochrome activities. Copper is an essential micronutrient required for the growth of wheat. Manganese is required for enzyme activation, in electron transport, and in disease resistance. The pot experiment was set up in greenhouse on calcareous chernozem soil Debrecen-Látókép with a spring wheat. In certain development stages (according to BBCH growth scale of wheat), at the beginning of stem elongation (29–30), at the heading (51–59), at the flowering (61–69) stage three average plants were removed from all pots for analysis. Fresh and dry weight of the plant samples were measured. Plant leaves after drying were digested by HNO3-H2O2 methods and manganese, zinc and copper contents of plant were quantified by atomic absorption spectrophotometry. At the flowering stage, when the nutrient uptake of plants is the most intensive, the weight of wheat ranged between 0.94–1.57 g plant-1. In this development stage, the NS2 treatment produced the highest weight of wheat, and compared to this the NS3 treatment decreased that value already. The results show unfavourable effect of NS3 treatment. On the basis of microelement content of wheat and the weight of a plant, nutrient uptake by plant were calculated. At the beginning of growth the starter treatment had positive effect on Cu-uptake compared to the NS1 treatment, where the same dose of fertilizer was stirred into the soil. Wheat is very sensitive to copper deficiency, so copper dissolved by starter treatment could be favourable to the early development of wheat. At flowering stage the Zn-uptake of wheat became the highest and it was between 133.7–234.6 mg plant-1. The Mn-uptake of wheat plant was higher than the Cu- and Zn-uptake of wheat.
This phenomenon can be explained by the fact that the untreated soil had higher Mn-content, than Cu- and Zn-content. To summarize the results, it can be stated, that the copper uptake of wheat was more affected by the different treatments in the stage of stem elongation, while Mn- and Zn-uptake of wheat were influenced primarily in the stage of heading and flowering.
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Organic soybean production in Hungary
45-52Views:475Organic soy production, particularly for feedstuff in organic animal husbandry, is needed in Hungary and the EU regions. Soybean producing crop area in Hungary has increased in the last years, however, the agroecological potential would allow for a larger soybean production area. This study presents the soybean market in Hungary, and the role of soybeans in protein supply in food and feed, summarising the critical elements of organic soybean production from variety selection to marketing. During the field research, the effects of the agronomical environment on yield potential factors were examined. We aimed to determine how different row spacing and tillage systems affect organic soybean yields of different varieties, with particular attention to the dry region, and determine the specific methods and varieties of soybean that favour these areas. We found that the tillage (plough/grubber), the spacing (wide/narrow), and the variety effects were all significant on morphological variables. The most remarkable difference was seen in plant height and the number of pods per plant, while the number of nodes was also highly impacted by tillage treatment and variety. The nutrition variables were significantly different, mainly as an effect of tillage and spacing interaction with significantly different plant responses of varieties.
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Possibilities of Growing Artichoke (Cynara scolymus L.) in Hungary
126-130Views:210The domestication of choice broadening, special vegetable species offers new market possibilities in Hungary. The production of artichokes has good perspectives due to its high protein, vitamin and mineral contents and its wide usability. According to the literature, in Hungary it is worth examining the annual production technology, which has yet to be researched in our country. The basic aim of our work was to establish the annual production of artichoke in Hungary with scientific research.
In the experiment, 5 Italian sorts (Valetta di Roma, Gobbo di Nizza, Violetto di Romagna, Violetto S. Francesino and Precoce di Romagna), 1 American sort (Green Globe) and 1 Egyptian sort (Balady) were used. For one half of the plants, we tried to bring on bud-production appearing in the first year only with early planting, while we used also a gibberellic acid hormone treatment for the other half.
Only the sort Green Globe had yields in appropriate quantity (21.4 t/ha) and quality. The low yield and inequable quality of buds of the Italian and Egyptian sorts refers to the fact that these sorts are not applicable for annual production in Hungary. Although the gibberellic acid treatment resulted an earlier harvest, increased the equality of buds and increased the proportion of stems producing buds except for the sort Green Globe, overall it did not have a positive effect. The hormone treatment fastened the physiological processes of the plants, thereby it induced early insenescence and lignifying of the buds. The high temperatures during the bud-formation period largely increased the ratio of flabby, over-blowing buds, so the planting must be timed in the future that the harvest period is in early automn or late spring, early summer months.
According to our results, globe artichoke can become a perspective culture by applying cold-resistant sorts with short growing seasons, which can be produced as annual crops without hormone treatment by the appropriate timing of bud-formation. -
Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
153-157Views:470Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.
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Response of maize seedlings to the latent zinc deficiency under different Fe/Zn rati
125-128Views:240Zinc (Zn) deficiency is a critical nutritional problem for plants and peoples all over the world. Almost half of the world’s cereal crops are deficient in Zn, leading to poor crop yield. In this study, the effect of different Fe/Zn ratio on some physiological parameters of maize seedlings were investigated on the dry matter of shoots and roots and their ratio, SPAD index and the total length of shoots. The relative chlorophyll contents significantly decreased under increasing Fe given to the Zn deficient. The results showed that the different Fe/Zn supplies decreased the total length of shoots - ranging from 9% to 65% - by latent Zn deficiency. Corresponding to several scientific findings, it was observed that the non-optimal Fe/Zn ratio of tissues take part in the evolution of latent Zn deficiency in the case of high Fe concentration.
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The effect of compost made of sheep manure on the first cut of a semi-natural grassland
25-29Views:400In an experiment, two types of compost were tested on natural grassland in order to improve the productivity of a natural sward on solonetz soil. Both composts were made of on-farm produced sheep manure, but the second one was enriched in phosphorus. These fertilizers/composts are officially authorized and can be applied in organic farming. Zero application and three rates of fertilizer were tested (10 t ha-1, 20 t ha-1, 30 t ha-1) on 30 m2 experimental plots wit four replications. Dry matter, crude protein and net energy content for maintenance were measured and determined and their yields per unit area were calculated. We found that all the treated plots produced significantly higher yield than the control ones, and in some cases the yields were almost three times higher. However, the optimal compost dose varied, depending on the examined parameter. Based on the evaluation of the experiment results along with rainfall data, it was concluded that, with higher precipitation, the positive effect of the compost application was bigger. The difference between the natural and enriched compost is remarkable, but it has to be mentioned that even the natural compost showed good results; therefore, it is also effective in improving the fertility of grassland.
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Changes in the Sulphur Content of Winter Wheat in a Field Experiment
85-88Views:201The use of superphosphate as P-containing fertiliser decreased in the last years in many countries in accordance with strict air pollution laws, and the S-deposition decreased from the atmosphere to the soil as well.
Winter wheat is the one of the S-demanding plants. Recently, the gradually increasing S absence endanger the formation of required average yield of winter wheat, and has bad effect on its quality.
We examined the effect of treatments on the sulphur-, nitrogen content and the N/S ratio of winter wheat in the whole upperground plant and in the grain and straw at harvest in a arable land sulphur fertilization experiment on brown forest soil (Agricultural Company of Felsőzsolca).
We analysed the samples from spring to harvesting, in the critical phenophases. In this study we discuss only the values from the stooling and stalking and the results of analysis of grain and straw in the harvest.
We experienced that the concentration of sulphur in the whole upperground parts of winter wheat showed increase to the end of vegetation independently of fertilization. The N/S ratio was between 8% and 12% in the beginning of the growth period in the whole upperground plant, while the ratio in the grain at harvest was between 13 and 14%. When we examined the whole upperground plant, stalk and leaf at stalking, we got the highest sulphur content in the leaf. Mostly the middle level sulphur fertilization dose (4 l/ha) increased the sulphur accumulation in the green plant. At total maturing, the greatest part of accumulated sulphur is in the grain, but then the effect of fertilization is less glaring. -
The examination of relation between the C/N ratio and the biogas yields in the Regional Biogas Plant of Nyírbátor
63-68Views:278The Regional Biogas Plant of Nyírbátor was built by the Bátortrade Ltd. The biogas plant contains 6 mezophil and 6 thermophil fermentation tanks, because the biogas production is based on mixed compositions. The regional plant is a multifunctional system. It produces agricultural products and biogas with high methane content. The utilization of biogas is also accomplished here; gas-engines transform it to electricity and
heat-energy. The product electricity is used by the local plants, the surplus is sold.
The aim of the research is the examination of the quality and quantity of the input materials that put into the mixers and follow the seasonal, periodical and optional changes of the input materials. The analyzation of the quality and quantity data can give an answer to the optional changes of biogas production because the input materials determine the composition of the examined recipe. The C/N ratio was between 11-13, the maximal value of the biogas yield was observed by 12.35 C/N ratio. -
Stability analysis of different winter wheat genotypes in long-term experiment
135-141Views:231Nowadays, due to the climate change, it is becoming increasingly important in the occasionally extreme years that the yield and the quality
parameters of a given winter wheat variety should not fluctuate at all or only slightly under similar agrotechnical conditions as a result of the year effect. In four years (2005–2008) we studied the changes in the wet gluten content, gluten speading and protein content of five wheat genotypes at six fertilization levels.
In the control, it can be observed that the year had a significant effect on the wet gluten content, the protein content of the flour and gluten speading, therefore, a great fluctuation was detected in these qualities of the varieties in the four studied years. At the optimum fertilization levels (N120-150+PK), the varieties Sixtus, Saturus and Lupus showed a much lower fluctuation and more stable values were measured. The most stable variety in the control treatment was Mv Mazurka for all the three quality parameters, while at the optimum fertilization level (N120+PK), the most stable results were obtained for the variety Sixtus as an average of the four years.
When studying the results using Kang’s method for stability analysis, it can be stated that the most stable values of wet gluten content were obtained at the fertilization level of N120+PK under a variable year effect, the varieties gave also the best gluten content values at this level. The most stable protein content values of flour were obtained at the fertilization level of N60+PK. The results showed that the fluctuation of quality parameters as a result of the changing years differed between the different winter wheat varieties due to their differing genotypes, but this fluctuation could be reduced or minimized by a proper fertilization. -
A földhasználat alakulása az ezredfordulón - egy felmérés eredményei
58-66Views:223There were no significant changes in land ownership and use in 2000, compared to the period following the compensation process. Land is mainly owned by people who do not want to use their property, so they generally turn to renting it out to others. Although farming on rented land is performed under various conditions, farms try to rent the lands of better quality. In general, examined farms would like to increase their size by tenancy or land purchase. Buying land is a good investment, considering the price increase in the future, but because of the lack of capital, tenancy remains the main form of territory increase.
Farms which rent land are in connection with a lot of owners, the land within their use is frittered away, it is in a lot of parts. Most rental contracts are written, but the ratio of oral contracts is still quite high. To reduce this ratio is one future aim. One more characteristic of the rental contracts is the dominance of a medium length period – which is acceptable for both the owner and user, but the ratio of short time contracts is still high. Land rent, on average for the examined farms, is quite balanced, it is on the level of about 16.000 HUF/hectare.
The land users mainly pay the same land rent to the owners, but in some cases, there are exceptions. Generally, land rent is a fix cost, but sometimes this cost depends on the level of the yield or the change of product price. Land rent, on average, is 16% within the production costs on the examined farms, which try to choose better and larger lands for rent. Sometimes, farmers can pay higher land rent for the unit of better land, but this is not a general tendency. It is mainly true that they pay the same land rent for the unit, regardless of land quality. Land owners can not interfere in the use of their land within the rental period, and also is a main characteristic of contracts that important particulars are not spelled out sufficiently by the partners. It follows that their content has to be improved in the future. -
Qualitative detection of genetically modified organisms in plant samples
309-313Views:625We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.
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Sour cherry anthracnose and possibilities of the control with special regard to resident Glomerella population in sour cherry plantations of East Hungary
12-17Views:297Anthracnose is considered one of the most destructive diseases for sour cherry production due to the rapid development of the disease on fruits. Glomerella cingulata (Stoneman) Spauld. & H. Schrenk (anam.: Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz.) has been the fungal pathogen responsible for anthracnose in last decades. Yield losses greater than 90% may occur under epidemic conditions. C. acutatum (J.H. Simmonds, 1968) strains were isolated of sourcherry plantations in East Hungary and this pathogen, new for Hungarian microbiont became recently dominant. Contrarily to the former species it is certainly transmitted with ants during fruit ripening. About third of strains proved to be cutinase producers that enable them to actively penetrate via cuticule, and these strains infect directly berries of blackberry, grape and tomato as well as plum and apple. Most of cutinase negative strains could also infect these fruits after mechanic injury. All strains of both species produce amylase, cellulase, lecithinase, lipase, polyfenoloxydase and protease in vitro, although the activity of these enzymes highly varied in the medium. The only C. acutatum strains produced noticeable amount of chitinase. Strains, tolerant to recently applied fungicides to control the anthracnose, could be isolated of sour cherry plantations that might be the cause of ineffectiveness of control measures in 2010. The mycofungicide containing mixture of three Trichoderma species in oil carrier could efficiently depress the development of anthracnose in ripening sour cherry.
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The role of green manure crops in Hungarian plant production
49-53Views:571According to the data of KSH (Hungarian Central Statistical Office), the sowing area of cereals in the crop year 2016 was over 2.56 million ha, on which winter wheat and maize were produced in a rate of around 50–50%. Regarding these data it is obvious that the domestic cropping structure has been simplified and become unilateral. This unfavourable crop rotation system causes several problems. The number of Hungarian livestock decreased in the past decades. The amount of manure was 24 million tons in 1960, but only hardly 4.5 million tons of organic manure was applied in 2016. Therefore, the importance of other possibilities, alternatives for organic matter recovery have become enhanced. This is especially important from the aspect of sustainable plant production. The fact that the European Union has introduced new directives for subsidiaries in 2015 has to be noted as well.
The objective of the set experiment is to find new technological solutions that are suitable for the execution of sustainable plant production by inadequate crop rotation, organic substance recovery conditions or under more unfavourable climatic conditions.
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Quality comparison of selected apple cultivars under ULO-storage
62-75Views:204Our examination was carried out in the 103 ha apple orchard of the Kasz-Coop Ltd.. The plantation works with modern varieties standing on M9 root stock, planted in a tree density of 2500 tree/ha and treated with professional integrated technology. The date of harvest is defined by a combination of several ripening analyses methods. The orchard has its own ULO-system warehouse.
Used in the experiment were four apple varieties: ’Idared’, ’Golden Reinders’, ’Jonagold’ and ’Braeburn’.
While under ULO-storage, we took 20-25 piece samples 6 times regarding the four varieties, from which 5-5 pieces were kept under three weeks’ shelf life, measured on a weekly bases. In our research, we analysed the dynamics of the flesh firmness and the refractometric value under ULO-storage and three weeks’ shelf life, and the standard deviation dynamics of the refractometric value under ULO-storage.
Analyses were based on the following measurements: flesh firmness, refractometric value and number of mature seeds.
We looked for morphological and morphological-quality correlations.
Through these we described the dynamics of the flesh firmness and the refractometric value under ULO-storage and three weeks’ shelf life for each variety. We referred to the stability of these attributes and to the dynamics of their change. Later, with the help of these, and also considering our practical experience related to the measurements, we characterised the four varieties.
We found a positive correlation between the seed number and the flesh firmness in the case of the ‘Idared’, ‘Jonagold’ and ‘Braeburn’ varieties, but no correlation was found in the case of ‘Golden Reinders’ in this concern. We visualized the standard deviation dynamics of the refractometric values under ULO-storage. This allowed us to categorise the varieties. -
Use of Clearfield technology in the sunflower
114-118Views:217Sunflower is one of the most important cultivated plants in Hungary. We carried out our research in 2009 with eight imidazolinone resistant hybrids and one conventional variety in order to compare the efficiency and selectivity of Clearfield technology to the conventional system. In the trial the Clearfield hybrids were treated by 3,5 l/ha Wing-P (pre) and 1,2 l/ha Pulsar 40 SL(post). The plot of the conventional variety was sprayed by 4,0 l/ha Wing-P and 0,5 l/ha Goal Duplo (pre).
Wing-P also had a poor effect because of the lack of rain. Pulsar 40 SL gave an excellent result against the 2-6 leaves of monocotyledonous and the dicotyledonous weeds. Only the well-developed Hibiscus trionum survived the treatment. The combination of Wing-P and the Goal Duplo herbicide provided poor result against the caracteristical weeds of the experimental area because of the lack of rain. Oxifluorfen with contact effect burned the leaves of the sunflower.