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Additional information for the interpretation of the practice of rural development in the light of the corporate relations of the Agricultural Centre, University of Debrecen in the North-Great Plain Region
65-69Views:63In the spirit of the Lisbon strategy, the European Union has set out the primary aims of innovation development and competitiveness. A potential method to reach these aims is to enhance the relation between science and practice and to apply scientific results. This is the case at the Agricultural Centre of the University of Debrecen as well, since the tighter and looser cooperations of the institution provide a scientific structure, which is now a significant prerequisite for Hungarian agro-innovation, primarily for rural development processes in the North Great Plain Region. The present study, based mostly on empirical findings, strives to analyze the field of activities in relation to rural development performed by the Agricultural Centre, University of Debrecen.
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Methane emission from Matsuo rice paddy field in light of different fertilizers, costs, profit and carbon credit
9-13Views:170Nowadays global warming is a major issue to our environment. This issue is generated by the modern human activities like industry and intensive agriculture. This research is about methane emission from rice paddy fields. The aim of the study is to lower the methane emission from the field with the help of using different type of fertilizers, whilst we keep in focus the efficient economic operation. The main experimental field is Matsuo paddy field, (Matsuo town, Sanbu city, Chiba prefecture) which is analyzed by the Chiba University’s soil science laboratory, they provided the data for this study. During the study three type of fertilizer was analyzed which are all organic and the control was a regular chemical fertilizer. For all fertilizers the cost and income of the production were calculated and the profit was weighted with the methane emission what a specific fertilizer produced during the cultivation. In the future if the organic fertilizers are in focus than it is necessary to find a new material what can be competitive with the chemical fertilizers in focus of GHG emission or find an alternative way of the usage of methane in biogas production.
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Yield components of hairy vetch (Vicia villosa Roth.) in different sowing technologies on acidic sandy soil
83-88Views:266Nearly a quarter of the agricultural utilized area of our country is made up of sandy soils. Sandy soils are poor in nutrients, and, therefore, the effectiveness of farming is basically determined by the method of maintaining soil fertility and the fertilization practice.
The hairy vetch called Vicia villosa Roth (Sandy Roth.), also known as a sand pioneer, plays a significant role in the exploitation of sandy soils. Its cultivation was started in Hungary in the late 1800s. It is primarily used as green fodder, most recently as a green manure and as a soil protection plant. The lupine is grown mainly as a supportive plant, which was previously rye, and today it is triticale. The ratio of the two plants to each other and the spatial location of plants depend on the method of sowing.
The aim of our work was to present the yields of some of the grain grown in different sowing methods and some of its crops.
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Adopt the tobacco plastid transformation technics in Hungary
73-76Views:80Under the GENOMNANOTECH Debrecen Regionális Egyetemi Tudásközpont (GND RET) research program in summer 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture experiments were launched to transform tobacco plant by plastid transformation technics.
Our aim was to adopt first in Hungary the tobacco plastid transformation technics, which were used in Waksman Istitute Rutgers, The state University of New Jersey (USA) leading with Prof Dr. Pál Maliga. Scientists won scholarships learn and use this technics in the University of Debrecen. -
Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:294The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.
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Angle-count sampling method for estimating forest stand volume – a practical approach
99-102Views:165Point sampling, which is also known as angle-count sampling (ACS), can be considered an efficient way of estimating the basal area and volume of forest stands. It is possible to use it in forest management: providing more accurate estimates (precision <10%) of site and stand characteristics needed for management planning. 20 black locust (Robinina pseudoacacia L.) stands were selected at final cutting age to determine the regeneration criteria based on their total volume. It was verified that at P=5% there was no difference between the main volume values of stands indicated in the relevant forest plans as well as calculated by the ACS method.
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Does drought stress always negatively affect the yield and quality of soybean in Hungary?
37-40Views:208Globally-fluctuating climate imposed serious abiotic stresses on the agricultural sector, leading to noticeable, and sometimes disastrous, losses in yields and/or quality of crops; however, in certain cases, plants could survive stress with relatively low reductions, and sometimes even with some enhancements as a reaction to changed environment, especially in the case of mild stress. An experiment was conducted in 2017 and 2018 in Debrecen, Hungary to evaluate the mild drought stress influence on the yield and quality of three soybean cultivars. The results showed that both ES Pallador and Pedro cultivars could achieve more yield when subjected to mild drought conditions; however, protein concentration was enhanced in ES Pallador whereas slightly degraded in Pedro under drought. The cultivar Pannonia Kincse followed different trend; both yield and protein concentration were reduced under drought. Oil concentration of the three cultivars did not show significant changes; however, it always followed opposite trend to that of protein concentration. It could be concluded that both ES Pallador and Pedro are recommended to be grown under rain fed conditions in Debrecen, whereas Pannonia Kincse is recommended under irrigation conditions.
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The effect of sowing date and plant density of winter oilseed rape (Brassica napus var. napus f. biennis L.) population
213-215Views:175The experiment has been set up in the University of Debrecen Látókép Experimental Station in three different years (2014, 2015 and 2016), three different plant densities 200, 350 and 500 thousand ha-1, four replications of the same nutrient supply with using a line spacing of 45 cm. In the experiment, the fore crop was winter wheat in each year. The amount of weeds was observed five times in the last experimental year (2016/2017). In the three experimental years, the highest yield was harvested from the early sowing plot with the highest plant density. On the basis of the Pearson’s correlation analysis there was significant negative correlation (r=-0.583) between the effect of the annual year and yield of the hybrid.
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Evaluating of soil sulphur forms changes in long-term field experiments of Látókép
71-76Views:161The aim of this work was to evaluate the changes of different sulphur forms (soluble, adsorbed) in chernozem soil in a long-term field experiment supplied with increasing doses of NPK fertilizers for a long time. In addition, other objective of this study included the examination of the applicability of recommended extractants of the different sulphate fraction in Hungarian soils. A long-term field experiment was established at the Research Station of Látókép of the University of Debrecen in 1984. In addition to control, two levels of NPK fertilizer doses have been used with irrigated and non-irrigated variants. Winter wheat and corn were cropped in a crop rotation on plots. Soil samples were collected in three different development stages of winter wheat, at the stage of stem elongation (April), flowering (May) and ripening (June of 2018) from the topsoil (0–20 cm) of experiment plots. Water-soluble inorganic sulphate was extracted with 0.01M CaCl2 solutions. The soluble plus adsorbed sulphate was extracted with 0.016M KH2PO4 solution. Sulphate was measured by turbidimetric method. 0.01M CaCl2-SO42— ranged between 0.293–1.896 mg kg-1 and the 0.016 M KH2PO4-SO42- varied between 5.087–10.261 mg kg-1. The values of KH2PO4 SO42- was higher than that of CaCl2-SO42-, because KH2PO4 extracted the adsorbed and soluble fractions of sulphate, while CaCl2 extracted the soluble sulphate fraction. The amount of absorbed sulphate was calculated by the differences of KH2PO4- SO4 and CaCl2-SO4. The KH2PO4 characterizes mainly the adsorbed sulphate fraction much more than the water-soluble fraction. KCl is the most widely used extractant for the determination of plant available sulphate content of soil in Hungary; therefore, KCl-SO42- fraction also was determined. The KCl-SO42- ranged between 0.328–2.152 mg kg-1. The CaCl2-SO42- and KCl-SO42- fractions were compared and based on Pearson's linear correlation, moderate correlation was established (r=0.511) between them. In all three extractant (0.01M CaCl2, 1M KCl, 0.016 M KH2PO4) higher sulphate fractions were measured in the fertilized plots where superphosphate had been supplied for ages until 2010. The arylsulphatase activity of soil also was determined and ranged between 9.284 and 26.860 µg p-nitrophenol g-1 h-1. The lowest value was observed in the treatment with highest NPK2 dose, both in irrigated and non-irrigated areas.
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The scientific background of competitive maize production
33-46Views:294The effect and interaction of crop production factors on maize yield has been examined for nearly 40 years at the Látókép Experiment Site of the University of Debrecen in a long-term field experiment that is unique and acknowledged in Europe. The research aim is to evaluate the effect of fertilisation, tillage, genotype, sowing, plant density, crop protection and irrigation. The analysis of the database of the examined period makes it possible to evaluate maize yield, as well as the effect of crop production factors and crop year, as well as the interaction between these factors.
Based on the different tillage methods, it can be concluded that autumn ploughing provides the highest yield, but its effect significantly differed in irrigated and non-irrigated treatments. The periodical application of strip tillage is justified in areas with favourable soil conditions and free from compated layers (e.g. strip – strip – ploughing – loosening). Under conditions prone to drought, but especially in several consecutive years, a plant density of 70–80 thousand crops per hectare should be used in the case of favourable precipitation supply, but 60 thousand crops per hectare should not be exceeded in dry crop years. The yield increasing effect of fertilisation is significant both under non-irrigated and irrigated conditions, but it is much more moderate in the non-irrigated treatment.
Selecting the optimum sowing date is of key importance from the aspect of maize yield, especially in dry crop years. Irrigation is not enough in itself without intensive nutrient management, since it may lead to yield decrease.
The results of research, development and innovation, which are based on the performed long-term field experiment, contribute to the production technological methods which provide an opportunity to use sowing seeds, fertilisers and pesticides in a regionally tailored and differentiated way, adapted to the specific needs of the given plot, as well as to plan each operation and to implement precision maize production.
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Effects of soil cultivation and environmental changes on maize yield
97-100Views:143We evaluated the relationships among soil cultivation and other agrotechnical factors (fertilization, number of plants and hybrid) within the framework of a multifactorial long-term experiment set at the Látókép Experimental Site of the Centre for Agricultural Sciences of the University of Debrecen in mid-heavy chalcareous chernozem soil based on a long-term experiment conducted for a 5-year period (2002–2006).
Based on the evaluation of soil cultivation by the average of treatments, it may be assessed that spring ploughing (8.204 t ha-1) provides more favourable conditions to the stand compared to spring shallow cultivation; however, this did not result in a significant difference. Spring ploughing considerably increased the yield of hybrid FAO 300 in dry years, whereas it considerably increased the yield of hybrid FAO 400 in favourable crop years. A stand of 70 thousand stems/ha provided the higher yield result in both soil cultivation types. It was sufficient to use a fertilizer dose of 120 kg N ha-1 for economical production.
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The effect of sowing date and plant density on the yield of maize (Zea mays L.) under different weather conditions
205-208Views:294Maize has high productivity and produces huge vegetative and generative phytomass, but this crop is very sensitive to agroecological (mainly to climatic, partly to pedological conditions) and agrotechnical circumstances. In Hungary, maize is grown on 1.1–1.2 million hectares, the national average yields vary between 4–7 t ha-1 depending on the year and the intensity of production technology. The longterm experiment was set up in 2015–2016 on chernozem soil in the Hajdúság (eastern Hungary). The maize research was set up on chernozem soil at the Látókép MÉK (Faculty of Agricultural and Food Sciences and Environmental Management) research area of the University of Debrecen. We examined the following commonly used hybrids of Hungary: SY ARIOSO (FAO 300), P9074 (FAO 310), P9486 (FAO 360), SY Octavius (FAO 400), GK Kenéz (FAO 410), DKC 4943 (FAO 410). The experiment was set up in three different plant densities. These were 60, 76, 90 thousand plant ha-1. The experiment was set up with three different sowing dates, early, average and late sowing. The yield was measured using a special plot harvester (Sampo Rosenlew 2010), measuring the weight of the harvested plot and also taking a sample from it. As a next step, we calculated the yield (t ha-1) of each plot at 14% of moisture content to compare them to each other. We evaluated the obtained data using Microsoft Excel 2015.
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Rheological evaluation of industrial tomato
137-140Views:122Today, tomato has the second largest harvesting area globally, and the fourth largest in Hungary. The importance of industrial tomato is growing year by year, and one of the most important tasks is to satisfy the needs of the industry. To meet these needs, the producer has to find the proper genotype, which can achieve up to 100 t ha-1 crop yield. This quality has high importance to be researched.
The experiment was carried out in the Botanical and Exhibition Garden of the University of Debrecen, with 3 examined genotypes. The planting date was 3 of May 2018. The harvesting was two-threaded. On 27 of July the physical parameters – fruit shape index (length/diameter), firmness (kg cm-2), water-soluble dry matter content (Brix%) and weight of the berries (g) – were evaluated. On 15 of August the rheological parameters were examined – the force needed to tear the skin – bioyield point (g) and flesh firmness (g).
The fruit shape index was oval (between 1.19 and 1.24) for all the examined genotypes. The firmness was between 0.92 (UG1122713 F1) and 1.05(Prestomech F1) kg cm-2, which compliance the needs of the industry. The water-soluble dry matter content was suitable, with a value between 4.49 (Prestomech F1) and 4.65 (UG1122713 F1) Brix%. The weight of the berries was between 45.94 (UG989 F1) and 49.37 (Prestomech F1) g per piece.
The bioyield point was between 76.00 (UG1122713 F1) and 85.16 (UG989 F1) g. The flesh firmness was between 33.68 (UG1122713 F1) and 42.81 (UG989 F1) g.
Finally, we can conclude based on the obtained data that the UG989 F1 hybrid was the most proper for the applied cultivation conditions. This genotype can be recommended for the farmers even in extreme weather conditions.
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Evaluation of dry matter accumulation of maize (Zea mays L.) hybrids
35-41Views:369The increase of the grain yield of maize is closely correlated with its seasonal dry matter accumulation. Dry matter is accumulated into the grain yield during the grain filling period. The following maize hybrids were involved in the experiment: Armagnac FAO 490, Loupiac FAO 380 and Sushi FAO 340. In order to determine dry matter content, two samples per week were taken on the following days: 22nd, 25th, 28th, 31st August, 4th, 7th, 14th, 18th, 22nd, 25th, 29th September and 2nd, 6th, 9th, 13th October. In the course of sampling the weight of 100 grains from the middle section of 4 ears was measured in 4 replications. Dry matter content was determined after drying to constant weight in a drying cabinet at 60 °C. Harvesting was performed on 13th October 2017.
The daily precipitation sum was determined by local measurements, while the daily radiation and temperature data were provided by the Meteorological Observatory Debrecen of the National Meteorological Service in Budapest. Among the agrometeorological parameters, an analysis was made of the precipitation during the growing season, effective heat sums during the vegetative and generative phase, and the water supplies. The daily heat sums were determined using the algorithm proposed.
The amount of precipitation in the winter period before the 2017 growing season was 210 mm. The soil was saturated until its field capacity. The rather dry and warm March and April had a favourable effect, but there was no worthy amount of precipitation until May (51 mm) due to the condition of the dried seedbed. Sowing was performed on the 5th of May 2017 in a randomised small plot experiment. There was favourable precipitation and temperature during the growing season, thereby providing ideal conditions for maize development, growth and yield formation. There was near average amount of precipitation in each year. The total amount of precipitation in the summer period is 342 mm. Temperature was mostly above the average, but there was no long and extremely warm period.
The Armagnac hybrid reached its highest dry matter mass 126 days after emergence. Physiological maturity was reached sooner (on the 119th day) in the case of Loupiac, and even sooner in the case of Sushi (116th day). The thousand grain weight of Sushi (which has the shortest ripening period) was 286 g at the time of physiological maturity, while that of Loupiac was 311 g. Compared to Sushi, Armagnac showed 12 g more dry matter accumulation (306 g). In the case of all three examined hybrids, physiological maturity was preceded by an intensive phase, when the dynamics of dry matter accumulation was rather quick. On average, Sushi gained 2.8 g dry matter per day between 103 days following emergence and physiological maturity, while the same values were 3.2 g for Armagnac and 3.3 g for Loupiac. The aim of the regression line slope is to predict the behavior of the dependent variable with the knowledge of the values and characteristics of the independent variables using the regression line equation. Furthermore, to determine how the location affected the dynamic of dry matter accumulation in the Armagnac, Loupiac and Sushi hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97%, in the Loupiac 94%, in the Sushi 90 %. The determination coefficient (R2) is useful in determing how the regression equation fits. But, as we have seen, the determination coefficient alone is not sufficient to verify the model’s accuracy, in addition to the determination coefficient (R2), the normality of the data or the residuals, the variance of the variables at different levels, the independence of the data relative to time and non-oblique. Observations are evaluated for the correctness of the fitted model.
Dry matter values decreased evenly and slightly following physiological maturity. According to our research results, it was established that physiological maturity is followed by a moderate dry matter loss. Until harvesting, Armagnac lost 40 g of its thousand mass weight in 29 days, while the same value pairs were 69 g in 36 days for Loupiac and 29 g in 39 days for Sushi. Loupiac – which had the highest weight at the time of physiological maturity – lost the most of its dry weight; therefore, Armagnac and Sushi had higher values at the time of harvesting.
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Evaluation of striptillage and conventional tillage in maize production
37-40Views:161Tillage changes soil properties and the way how the environment affects those properties. Soil properties and environment determine the rate of water movement in liquid and gaseous form into and out of soil. Based on the experimental database of the Institute of Land Utilisation, Regional Development and Technology of the University of Debrecen, Faculty of Agricultural and Food Sciences and Environmental Management and the KITE PLC, various cultivation systems were examined with maize (Zea mays L.) as indicator plant in Jász-Nagykun-Szolnok county. The sample area can be found in the outskirts of Kenderes on a meadow chernozem soil. On the examined plot, strip-tillage, subsoiling and moldboard ploughing were performed, each on 4.5 ha, respectively. The purpose of the present study is to compare these cultivation systems according to the soil- and maize kernel moisture content and to the yield based on the years of 2012 and 2013.
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Impact of tillage systems on maize emergence
129-136Views:63In Europe, there has been a significant change in the way tillage is approached in recent years. This change is due to a growing awareness among farmers, politicians and society as a whole that soil is not a renewable resource in itself. From an agricultural point of view, the greatest impact on soil condition can be achieved through the use of the applied tillage systems. My research takes this approach as a basis when examining the different tillage systems and their impact on the environment. In this context, conventional and a variety of no-tillage systems are examined in this paper. As a next step, it is examined how the environmental conditions created by the different tillage systems influence the emergence of maize hybrids. The analyses are carried out in a multi-factorial, long-term tillage field experiment. The same batch of the same hybrid seed was sown in several crop years, and the effects of environmental conditions on the emergence process were examined. Environmental effects and emergence-related uptake were measured in the examined plots. Measurements of environmental effects included air temperature, precipitation, soil temperature measured at seeding depth, as well as % cover of stem residue on the surface in the treated plots. The first emergence time measurements of the sown crop in the plots of each treatment were compared and relationships between these factors were investigated.
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The development of the scientific bases of horticulture and the history of horticultural innovation in the Northern Great Plain region and in Debrecen
17-20Views:79It can be told about the second half of the XXth century that, apart from preferring the large-scale growing of field plants and the largescale
livestock farming, corresponding to the central political will of the communist era, the significance and innovation output of
horticultural education in Debrecen was rather of follow-up trait, of secondary importance. The Tobacco Research Institute continued the
research work, and then even this activity was stopped. According to a survey finished in 1997, the mentioned institute had no invention,
granted patent, protection for registered model or any application for patent in progress at the Hungarian Patent Office. Until this time, invention activity at the University of Agricultural Sciences was of medium standard. In the National Patent Office, seven patent applications related to agricultural production and nine patent applications for other fields submitted under the inventors’ names were recorded. In the same period, the Cereal Research Institute (Szeged) led the absolute innovation list of Hungarian agricultural R+D institutions and university education institutes by submitting 164 own patent applications. Both in domestic and international terms, the horticultural innovation conducted at the University of Agricultural Sciences, despite the individual research results and achievements deserving recognition, without appropriate background – remained unnoticed. Let us put it this way: for the past decades, the light of the Debrecen Flower Carnival has not been thrown on the horticultural teachers and researchers of Debrecen. -
Characterisation of basic water balance parameters of Debrecen
35-40Views:200This work aims to develop a hydrological modelling tool to help managers make the right decisions for Debrecen, in the face of water scarcity and the increase in agricultural and domestic needs over time. The methodology was based on the creation of a climatic database, at monthly time steps, from 2016 to 2019, and cartographic (land use, digital elevation model, and hydrological network). As a next step, the watershed was delimitated into sub-basins to determine the shape and the physiographic characteristics of sub-watersheds. Finally, a hydrological study was prepared by calculating the time of concentration to build a database of water resources in the study area. This water resource will be used as an input parameter for urban farming.
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Results of the sensory analysis of precision maize production
59-62Views:194This research was carried out in 2018, at the Látókép Experimental Station of the University of Debrecen in a moderately warm and dry production area, on deep humus layered medium-hard calcareous chernozem soil. In the scope of the research, the chlorophyll content of maize (Zea mays L.) was examined under field circumstances by means of local sensory measurements and we were looking for correlation between the obtained values and the amount of yield. Our measurements were carried out with Minolta SPAD-502 and GreenSeeker devices at 3 measurement times (4 leaf stage, 10 leaf stage and silking). It was found that phenological phases had an effect on the obtained SPAD and NDVI values and were in a slightly significant correlation with the yield. The most significant correlation was found between the results obtained during silking and the amount of yield. This may be because the least time has passed between the measurement time and harvest. Results obtained during the 10-leaf stage show excessive values in each case, which can be due to a measurement error. It was found that the phenological phase had an effect on the correlation of SPAD and NDVI values and the amount of yield. As the phenological phase progressed, the correlation between the measured results and yield has increased.
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Interpretation of rurality and the situation of land use in Hungary
79-85Views:189The summarizing data collection of our study has been carried out in the scope of the FP7-REGPOT-2010-1 ’UD_AGR_REPO’ project as a part of the cooperation with the University of Lincoln. The University of Lincoln is an important partner of the project, the knowledge transfer activities that have been carried jointly with them are multilateral. One of the most important cooperation areas is the analysis of rural areas, rurality itself, determination of breakout points, exploration of alternative income sources, diversification possibilities. Some part of the work of the University of Lincoln on the field of rural development is based on the assessment and documentation global similarities and differences of rural areas. Present study also contributes to that work, it has been prepared on the request of the University of Lincoln with the aim of providing insight into the special political and economic changes/processes that took place in Hungary, and through them into the structure and operation of the unique Hungarian rural areas.
The study first positions the definition of rurality and rural areas into context on the basis of official EU and Hungarian legal classification. Then it covers the important agricultural nature of Hungary, which significantly determines the possibilities and properties of Hungarian rural areas. The further description of rural areas is completed by some historical summary, the introduction of ownership changes, detailed description of employment and income conditions and finally by the listing of breakout points of rural areas as a conclusion.
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Effects of long-term K fertilization and liming on the extractable and exchangeable K contents of a Haplic Phaeosem soil
141-145Views:35Effects of regular K fertilization and liming on the easily extractable K content of a Haplic phaeosem soil determined in 0.01 M CaCl2
and AL (traditional method in Hungary) were examined in the B1740 type of the National Uniformed Long-Term Fertilization Experiments
at Karcag.
Close correlation (r=0.95) was found between the 0.01 M CaCl2 and ammonium lactate - acetic acid (AL) extractable K contents of
soils.
K fertilization increased the amount of 0.01 M CaCl2 and AL extractable K significantly. Liming had different effects on the amounts of
K extracted by these two methods. Liming increased the amount of AL-K and decreased the amount of CaCl2-K. CaCl2 extractable K was in
close correlation with the relative amount of exchangeable K content of the soil (K%) and the agronomic K balance. The results of regression
analysis confirmed that the CaCl2-K characterized K% and the AL-K related to the absolute amount of exchangeable K.
On the basis of the presented results it can be stated that the 0.01 M CaCl2 is able to detect not just the increase of easily extractable K
caused by fertilization and liming but the changing of the rate of the relative amount of exchangeable K. -
Effect of anthocyanin-rich sour cherry extract on the level of IL-8 in LPS-induced endothelial cell
27-30Views:203The anthocyanin content of the Hungarian sour cherry is remarkable. Nutraceutical and pharmaceutical effects of the anthocyanins and their role in disease prevention have been studied extensively. Endothelial cells are involved in the pathogenesis of several inflammatory diseases. The objective of this work was to investigate pure sour cherry extract on human umbilical cord vein endothelial cells (HUVECs) as an inflammatory model. HUVECs were treated with 100 ng/mL lipopolysaccharide (LPS) and 50 mg/mL sour cherry extract or M199 medium as control. The optimal concentration range of the sour cherry extract was investigated and selected based on MTT assay measuring the conversion of the tetrazolium salt to formazan by mitochondrial dehydrogenases. The level of interleukine-8 (IL-8), a pro-inflammatory cytokine, was measured in Luminex MagPlex assay. LPS treatment significantly increased the secretion of IL-8. The pure sour cherry extract was able to attenuate this increment indicating the potent anti-inflammatory effect of pure sour cherry extract. Our results emphasize that pure sour cherry extract could reduce the LPS-induced inflammatory response thereby may improve endothelial dysfunction.
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Effect of hail net on the water potential of an apple orchard
109-113Views:147Apple production has seen a decline in yields in recent years, primarily due to ecological factors such as drought, water stress, water scarcity, uneven rainfall distribution, frost damage and hail damage. Ecological factors that are harmful to the plant can be eliminated by human intervention, irrigation or the use of hail net. In our study, we investigated the positive effects of hail net on the development of plant water potential as the vegetation progressed, in non-irrigation area with temperature and humidity. Water potential values were determined using a field osmometer. Confirmed the positive effect of the ice protection net in the apple orchard of Early Gold and Golden Reinders. Our results were supported by statistical analyzes our results.
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Bacteria in the milk of sheep with or without mastitis- mini Review
47-52Views:338From a nutritional point of view, sheep milk is more valuable than cow and goat milk and the interest for sheep milk is increasing in many countries. However, sheep milk is easily contaminated during milking, handling, and transport and it is an ideal medium for bacterial propagation. Consequently, sheep milk spoils quite quickly. The proper, clean handling of milk is not only of sanitarian interest, but it also serves the farmers’ interests, because contaminated milk may not be distributed, and is unsuitable for producing good quality products. Following this technological trend, this review addresses the bacterial composition of sheep milk with and without mastitis. Even though sheep milk contains a lot of bacteria, this review article highlighted total plate count, Enterobacteriaceae, coliform, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Campylobacter, Salmonella spp. and Streptococcus spp. Mastitis in sheep is a vital cause of mortality, reduction in milk production and early culling. The reported risk factors for mastitis in sheep were age, a case of mastitis, breed, husbandry systems, and location. The main priority should be implementation of programs to minimize human pathogenic bacteria and mastitis in raw ewe milk.
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Long-term effect of soil management on the carbon-dioxide emission of the soil
515-527Views:99CO2 emission from soils is one of the most important elements of the global carbon cycle, thus it has crucial rule in climate change. Each soil cultivation operation intervenes in the microbiological life of the soil, hence tillage is a factor through that the processes taking place in soil can be controlled. During the last decades, the organic material content of agricultural soils decreased to the half due to the intensive management resulting in the degradation of natural soil fertility. While intensive, plough-based tillage can cause soil degradation and erosion, the physical, chemical and biological status of the soil can be significantly improved through the application of conservation tillage methods. The results of long-term experiments prove that soil protective tillage enhances the enrichment of organic matter in the top layer of the soil. In order to reveal the role of tillage systems in CO2 emission from the soil, regular measurements were carried out in the plots with conventional and reduced tillage of the soil cultivation experiment of Research Institute of Karcag. Anagas CD 98 and Gas Alert Micro 5w infrared gas analysers were used to measure CO2-concentrations, and a specially developed method (consisting of a frame and a bowl) was applied to delimitate the measuring area. Most of the measurements were done on stubbles after harvest in order to exclude root respiration. The weather conditions of the examined 10 years were very changeable providing a good chance to compare them to each other. We found the tillage operations resulting in higher emission values in both tillage systems. On stubbles higher and more even emission was characteristic to reduced tillage due to the lower degree of soil disturbance and higher soil moisture content.