No 69 (2016)

Published March 23, 2016

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Articles

Impact of lameness on the milk production of ewes
5-7

The aim of this study was assessing the impact of lameness on the milk production, somatic cells count and component of milk. We assess also impact of lameness on the order of entry into the milking parlour.

The experiment was carried at the farm, located in northern Slovakia. The farm keeps sheep Improved Valachian. Samples of milk wer...e taken during two periods: May, July. It was taken 428 samples together. We recorded three groups by lameness- strong lame, slightly lame, non-lame ewes. We recorded also the order of entry of ewes into the milking parlour in milking row. The results were mathematically processed using the Microsoft Excel program and statistically evaluated by SAS.

We found significant statistical differences between months (P<0.0001) in all the above mentioned indicators. In July we recorded 26 ewes with slightly lameness and 18 ewes with strong lameness. Other ewes were non-lame. Non-lame sheep had in July the highest milk yield (356±148 ml) and the lowest decrease in milk yield from May to July (-206±131 ml) compared with slightly (317±116 ml, -223±163) and strong (319±122 ml, -219±151 ml) lame ewes. However, these differences were not statistically significant. We have not identified statistically significant differences between groups in somatic cells count (logxSCC for non-lame: 4.83±0.608 in ml, slightly lame: 4.76±0.653 in ml, strong lame 4.71±0.787 in ml). Milk composition (fat, proteins, lactose) nor changes in the composition of milk that occurred between May and July were not affected by lameness of ewes. But lameness in July affected the change the order of entry of ewes in the milking parlour in July compared with the order of entry recorded in May.

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Methane emission from Matsuo rice paddy field in light of different fertilizers, costs, profit and carbon credit
9-13

Nowadays global warming is a major issue to our environment. This issue is generated by the modern human activities like industry and intensive agriculture. This research is about methane emission from rice paddy fields. The aim of the study is to lower the methane emission from the field with the help of using different type of fertilizers, wh...ilst we keep in focus the efficient economic operation. The main experimental field is Matsuo paddy field, (Matsuo town, Sanbu city, Chiba prefecture) which is analyzed by the Chiba University’s soil science laboratory, they provided the data for this study. During the study three type of fertilizer was analyzed which are all organic and the control was a regular chemical fertilizer. For all fertilizers the cost and income of the production were calculated and the profit was weighted with the methane emission what a specific fertilizer produced during the cultivation. In the future if the organic fertilizers are in focus than it is necessary to find a new material what can be competitive with the chemical fertilizers in focus of GHG emission or find an alternative way of the usage of methane in biogas production.

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Some population genetics parameters of the present Hungarian Hucul Horse population
15-22

We examined the Hungarian population of the Hucul horse breed, under genetic protection, based on population genetic indicators until the year 2014 included. The evaluation was performed using the Endog programme based on the following indicators: inbreeding coefficient, average relatedness, the maximum number of generations, the number of full... generations traced and offspring number. Our findings were as follows: the average inbreeding coefficient of the total population was 5.99%, average relatedness was 11.82%, the maximum number of generations was, on the average, 16.04%, and the number of full generations traced with reference to the whole population was 4.15% on the average. 40% of the whole population (723 individuals) did not have any offspring; 42% (759 individuals) attained an offspring of 1 or 2, while 3.4% (88 individuals) had a surviving offspring of 3. The highest offspring number according to the national database (92) was attained by one stallion.

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Mitochondrial DNA-based diversity study of Hungarian brown hares (Lepus europaeus Pallas 1778)
23-29

The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.

The aim of our large scale long term ongoing study is to fill this gap of informati...on on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.

This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.

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Survival Analysis of Hungarian Large White, Duroc and Pietrain Sows
31-36

The aim of the present study was to perform lifetime performance analysis in three pig breeds; Hungarian Large White (n=295), Duroc (n=76) and Pietrain (n=91) on a commercial farm using analysis of survival sows. We took into consideration the age of sows at the time of their inclusion into breeding, their age at the time of culling, time spent... in production, number of mating and parities, parity percentage, intervals between litters, number and mean of piglets born alive and born dead, number of raised piglet litters, number and mean of 21 days old piglets, the weight and mean of raised litter and raise percentage.

We carried out the analysis by SPSS 22.0. Single factor analysis of variants, Kaplan-Meier analysis and Cox PH model were used. The determination of the significance of risk rates differences was done by Wald chi square test.

Our results showed that the average culling age were 1056 (±33.52) days for the Hungarian Large White, 735 (±73.56) days for Duroc and 818 (±71.98) days for the Pietrain.

The log rank test of the survival analysis indicated a significant difference between the three tested genotypes (χ2=16.981, P<0.001), which means that the survival percentage of the individual breeds varied significantly from one another. In comparison with the Hungarian Large White genotype the Duroc genotype has a 1.6 times higher (P<0.001) culling risk while that of the genotype Pietrain was 1.36 times higher (P<0.001).

Our results can be used to compare the breeds kept under the same conditions and to compare the life span of one genotype under different farming conditions. Factors that increase survival and improve the profitability of pig farming can be determined by this method.

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Application of molecular genetic methods for birds (literature review)
37-41

Ornithology studies have been extended by molecular genetic techniques. In this paper we are dealing with the most common use of areas. Our basic aim is to give a comprehensive view of the most commonly used methods in ornithological studies, including the available results by their use. We also deal with the following areas: an essential step ...during examination namely the applicability of sample collecting methods, and the unique identification, also the sex determination, methodology of DNA barcoding, as well as the role of molecular methods to protect endangered species. We discussed the advantages and disadvantages of the methods, such as the current trend for each method.

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Micropropagation of Leuce-poplar clones and its role in selection breeding
43-48
Leuce-poplars (mostly white poplar and its natural hybrid grey poplar) are native tree species trough Hungary. They are covering more than 4.0 per cent of the Hungarian forested area (77 000 ha). The white (grey) poplars play a significant role in the forestation under sandy soil site conditions as well as they are of importance from nature c...onservation point of view as well.
Long-term selection breeding work is going on at the National Agricultural Research and Innovation Centre Forest Research Institute (NARIC FRI), involving selection of fast-growing Leuce-poplar clones under dry site conditions.
Micropropagation technology is relatively quickly spreading in forestry. In vitro multiplication of trees is applied mainly in fruit growing in Hungary, in case of forestry it is used mainly for selection breeding.
This paper presents a short overview on the micropropagation trials with different Leuce-poplar clones and the early evaluation of the seedlings growing of the micropropagated clones/varieties.
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Evaluation of the blood product characteristics of meat meal and hemoglobin with non-invasive methods in the VISNIR wavelength
49-56

 

The separate collection of poultry slaughterhouse trimmings and blood is partially solved in Hungary. Only properly prepared animal by-products, protein meals can be utilized as animal feed additive. Howev...er, different protein meals are appropriate for feeding different animal species. That is the reason why it is important to avoid accidental cross contamination of the products. Meat and blood meal produced on the same technological line, therefore mixing of the products can happen in various proportions during the shift of production.

 

Thus the aim of this study is to develop a spectral method which will allow to estimate the ratio of meat and blood protein meal in the final product. During the test the products were mixed in different proportions and were examined by the spectral method. Measurements were conducted with AvaSpec 2048 spectrometer in visible (VIS) and in near infrared (NIR) wavelength range (400–1000 nm) to define the spectral differentiation of the different meal products. Significant difference can be detected in spectral reflectance between the meat and blood product in the VIS-NIR range. The blood product has a characteristic spectral property: in the range of 600 and 735 nm reflectance values are increasing following a sigmoid curve. This property is not observed in the case of meat meal: close to linear rising is detected. Effective protein rate and purity detection could be made by Blood Product Sensitive Mixing Index (BPSMI – R930/R600), and by the calculation of inflection point in 600–735 nm.

 

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Examination of microsatellite markers of Dorper sheep breed
57-61

Number of not woolly and molty sheep exceeds 60 million throughout the world. Their numbers and their importance is growing, still they have appeared in the past two decades all over in North-America, Australia, New-Zealand and also in Europe. The South African Dorper has been a pioneer among them in Hungary. It was introduced in 2006 in the co...untry. The Dorper sheep is the second largest breed in South Africa, which was developed from the crossing of Dorset Horn and the Blackhead Persian. The aim of the EU Member States in terms of this specific breed is increasing the small populations, improving the productive qualities, in addition to this avoiding inbreeding. However, finding appropriate breeding stock is difficult due to the small size of available populations and also to the suspected common of origin. With the help of various molecular genetic methods we could get a total view of the genetic background of these flocks. Nowadays the most commonly known and used genetic markers are microsatellites, because their applications give fast, accurate and easily reproducible results. There is no specific descriptive information on the genetic background of Dorper populations in the various EU countries , also regarding diversity between populations. Therefore in our work we want to optimize the conditions of applicability of 31 selected microsatellite reactions as a first step of mapping the entire genetic background of the different EU Dorper populations.

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Nutrition reaction of different varieties of winter wheat (Triticum aestivum L.) bred in Karcag
63-67

In our study the nutrition reaction of four varieties of winter wheat (KG Bendegúz, KG Kunhalom, KG Kunkapitány, KG Széphalom) has been investigated. In the experiment the effect of twenty different nutrition doses on the yield and thousand kernel weight of the wheat varieties has been studied. Significant difference could be figured out amo...ng the yield and thousand kernel weight of the wheat varieties, so the choice of the proper variety determines the quantity of yield. KG Kunkapitány had the highest yield, while KG Széphalom had the highest thousand kernel weight in the experiments.

Investigating the effect of the nutrients on the yield the conclusion was that all nitrogen doses had significant yield increasing effect compared to the untreated plots, but among the 40, 80 and 120 kg ha-1 doses there was no statistical difference. In the case of phosphorus the 40 kg ha-1 dose showed statistically verifiable increase of the yield, while potassium doses have no influence on the yields. The thousand kernel weights reached the maximum values where the highest nutrient doses were applied.

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Comparison of major population parameters of Brown hare (Lepus europaeus, Pallas 1758) in two hunting fields of the Great Hungarian Plain
69-74

Hungarian stock of game is not only part of our national treasure but also one of our domestic products and economic income. Not indifferent therefore the number and the state of health of our wildlife. Population decline of the Brown hare (Lepus europaeus, Pallas 1758) (one of our most important small game in Hungary) takes a long time. Demogr...aphic parameters of Brown hare was examined, particularly the factors affecting the decline of the species in Hungary. We took samples from typical habitats where the Brown hare could be found in relatively high density in our country. The article reports data of reproductive characteristics, diseases, parasites of Brown hare and other factors such as climatic and anthropogenic which could influence of the population dynamics. We mention sample collection and processing methods eg: population size estimates, examination of reproductive organs, the sex ratio and the age structure as well as the individual condition based on data of domestic and foreign authors and our partial results.

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Significance of PSI re-use
75-78

The prime aim of this study is draw attention to the importance of the issue connection with PSI reuse, to give a survey of the attepts carried out so far, legal impoudments. With the help of this we are trying to systematize conceptual basic as well as to expand the fields researched. Regarding PSI reuse the 89/2003 Directives of EU serve far ...the prime factor, as well as the LXIII. act of 2012. Which is the adoptation of the directive in Hungary including its modfications.

In Hungary a new governmental strategy is being formed, which would make the widespread use of PSI possible. Doing so via their reuse it would contribute to establishing new products and services. By this means it would greathy support the improving competitivness of the Hungarian economy.

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Examination of the conditions of extreme water balance circumstances (water logging, drought) with environmental information technology tools
79-86

The Carpathian Basin is characterized by varying hydrological extremes, both in space and time. Hungary's natural endowments are more favourable than average, especially for agricultural production, with 5,3 million hectares of land we have which is suitable for agricultural production. These extreme water management are often occur in the same... year and mostly in the same region, which may become more frequent in the future, especially in the lowland regions. The negative impacts of extreme water management was influenced by the land use changes in recent years, which has modified the runoff processes of the affected regions.

The aim of the study was to research the formation of inland water and drought circumstances in two sample areas the Great Plain (Szolnok-Túri flat and Nyírség) by geoinformatic tools. During the investigation in the first step we determined that areas which are susceptible to inland water and drought, based on the AGROTOPO database. In addition, land-use categories of characteristics of the sample areas are evaluated according to the Corine Land Cover. Furthermore, after defining characteristic of NDVI values between the period of 2003–2013, we evaluated the effect of drought whether can be detected in crop failures in respective areas.

Based on our results, we concluded that the formation of inland water and drought circumstances can be investigated in a large spatial extension by geoinformatic tools and databases.

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Examination the stresseffect of litter’s let-together system (“kindergarten”) by measuring faecal cortisol metabolites of sows and piglets
87-90

Piglets in commercial intensive pig husbandry are often abruptly weaned between 3 and 4 weeks for economic reasons. The process of weaning is a multifactorial stressor, in which nutritional, social, physical and psychologic stressors are combined. Piglets are often exposed to unfamiliar piglets around weaning which results in a period of vigoro...us fighting. Stress plays an important part in welfare research. Traditionally glucocorticoids are measured in blood samples but their use is often limited as the act of sample collection may stress an animal. Measurement of faecal cortisol/corticosterone metabolites is a non-invasive method for evaluation adrenocortical activity. The aim of this study was to examine the effect of litter’s let-together system (“kindergarten”) in the farrowing house by measuring faecal cortisol metabolites. According to our results the “kindergarten” system has no stress effect on sows and piglets, respectively.

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Sorghum and millet as alternative grains in nutrition
91-95

Sorghum (Sorghum bicolor L.) and millet (Panicum miliaceum L.) are the fifth and sixth most important cereal crops in the world. Gluten-free grains, therefore persons with coeliac disease could consume them also. In addition, they have a lot of positive effects due to their phenolic compounds (phenol acid, flavonoid, tannin). The total phenol c...ontent of sorghum is high, but Panicum miliaceum and Eleusine coracana have higher antioxidant activity. Fiber and mineral contents are also high, the protein contents are also higher than in standard cereals. Sorghum use is similar to corn: starch, glucose, syrup, and oil can be produced. Moreover, it can be used in preparing whole grain products, bread, pancake, dumpling, mush, cake, pasta and beer from sorghum. Broom and forage are also can be prepeared from them. Millet used such as mush, steamed food, cake, bread, alcoholic and non-alcoholic beverages.

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Evaluation of critical factors determining the profitability of sweet maize
97-102

The main goal of this analysis was to determine, whether the production of sweet corn competitive is from the point of view of profitability and identify the main factors determining profitability. The hypothesis of this research was that sweet corn production is profitable and output factors (yields, selling prices) affect most significantly t...he profitability. The total costs of production with irrigation are 560 000 HUF ha-1. Average yield is 18 t ha-1, while selling prices on average of the last 5 years were about 38 000 HUF t-1. The realisable profit in the sector was 248 828 HUF ha-1. Direct cost-related profitability was 48.6% and profit level 26%. Elasticity analysis pointed out that the changes of selling prices and yields affect profitability. The critical value shows the turning point of profitability, which was at the yield of 13.82 t ha-1. It can be concluded, the hypothesis was true, because sweet corn production was profitable compared to other field crops.

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The effect of NPK fertilization and the number of plants on the yield of maize hybrids with different genetic base in half-industrial experiment
103-108

In our research we examined the effect of the hybrid, the nutrient supply, the number of plants and the abiotic factors (temperature, amount of precipitation) on the yield, crop quality and yield stability of maize. We devoted special attention to the natural nutrient utilization ability and fertilizer reaction of maize.

The experiment ...took place in Hajdúszoboszló on chernozem soil, on a nearly 8 ha field. The size of one plot was 206 m2, this it was a halfindustrial experiment. We tested six hybrids with different genetic characteristics and growing seasons. I analysed the correlation between the nutrient supply and the yield of maize hybrids with control treatment (treatment without fertilization) and with N 80, P2O5 60, K2O 70 kg ha-1 and N 160, P2O5 120, K2O 140 kg ha-1 fertilizer treatments. Yield increasing effect of the fertilizer also depended on the number of plants per hectare at a great extent. The number of plants of the six tested hybrids was 60, 70, and 80 thousand plants/ha.

In Hajdúszoboszló, in 2015 the amount of rainfall from January to October was 340.3 mm, which was less than the average of 30 years by 105.5 mm. This year was not only draughty but it was also extremely hot, as the average temperature was higher by 1.7 °C than the average of 30 years. In the critical months of the growing season the distribution of precipitation was unfavourable for maize: in June the amount of rainfall was less by 31mm and in July by 42 mm than the average of many years.

Unfavourable effects of the weather of year 2015 were reflected also by our experimental data. The yield of hybrids without fertilization changed between 5.28–7.13 t ha-1 depending on the number of plants.

It can be associated also with the unfavourable crop year that the yield of the six tested hybrids is 6.33 t ha-1 in the average of the stand density of 60, 70 and 80 thousand plants per hectare without fertilization, while it is 7.14 t ha-1 with N80+PK fertilizer treatment. That increase in the yield is only 0.81 t ha-1, but it is significant. Due to the especially draughty weather the yield increasing effect of fertilizers was moderate. In the average of the hybrids and the number of plants, increasing the N80+PK treatment to N160+PK, the yield did not increase but decreased, which is explicable by the water scarcity in the period of flowering, fertilization and grain filling.

The agroecological optimum of fertilization was N 80, P2O5 60 and K2O 70 kg ha-1. Due to the intense water scarcity, increased fertilization caused decrease in the yield. As for the number of plants, 70 000 plants ha-1 proved to be the optimum, and the further increase of the number of plants caused decrease in the yield.

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The role of mediation resources in the operation and development of knowledgeintensive small and medium enterprises
109-113

Address social and economic processes of social capital system between universities and the business sector – because of their special and similar characteristics – major emphasis will be displayed. Knowledge-intensive companies would be hard to imagine functioning without established links with tertiary education institutions and universit...ies can now functions would be unthinkable without displaying the corporate sector sectoral specificities of knowledge and strategies of the university.

Because of the development of knowledge-intensive business activities based on innovation-oriented economic development which are specific to innovative SMEs, funding sources and opportunities. In a typical operating environment of innovation, with sectoral, spatial and temporal factors are constantly changing. These factors are decisive elements in the innovation opportunities, and through this indirectly the success of these enterprises. The resources involved in mediating the markets perceive a high risk to price this innovation activities and their funding. Under the study to try to answer that by optimizing the functions of universities, how and in what form they may have a role in mitigating financial risks.

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The economic structure and the tertiary ecucation of the function of transformation Hungary 19th and 20th century of in economic integration periods
115-122

Knowledge-intensive businesses sectoral nature of the operation and the specific attributes of the innovation life cycle processes, different needs and different needs induce. These features can also differentiate time that their activities when and what type of resources they require. The availability of capacity in terms of a network co-benef...its for those businesses that are creating the possibility that such resources are available to ensure the realization of goals that are not individually owned. These factors and the availability of local availability is also motivated by the small and medium enterprises willingness to cooperate.

Regional supply of resources necessary for the operation of the business of insurance, universities play a key role. The functions of universities in innovation as a business service can be understood as an activity that mediate high added value to other organizations and companies. Questions arise as to the innovative nature of the business industry and sector weights would differentiate whether the R&D&Iintensive areas of expertise.

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The characteristics of the resource needs of innovative businesses
123-127

Every university was funded in different historical periods with particular feature, particular political system, particular proprietory structure and particular economic background, which characterised the particular era. The historical antecedents considerably influenced the situation and role of the institutions as well as the course of thei...r development. Although they had common features but their spatial projections are very dissimilar. In the 19th and 20th century Hungarian history – in the periods of economic integration with the modification of political system and transformation of the social background – the economic and social functions of tertiary education underwent considerable changes, which started to accomplish by the second half of the 20th century and the early 21st century. To moderate regional disparities, European and Hungarian regional development policy considers particular importance to the economic structure of the regions and their potential to be reformed, which is one of the corner stones of compatibility. Considering the more and more diversifying functions of universities, the question is, which factor is more significant; tertiary education or the relation between the sectors of national economy. The possible correlations we presented through the economic structure and the transformation of tertiary education functions of the integration periods.

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Market competition and concentration in the world market of concentrated apple juice
129-135

In the study, I examined the market competition and concentration in the word market of concentrated apple juice. I used RCA index to determine comparative advantages In the world China possesses the strongest comparative advantages. Besides China, Poland, Hungary, Chile, Ukraine, Turkey and Moldova possess strong comparative advantages too. I ...used Herfindahl-Hirschman-index to determine the market concentration. Based on it – excluding the high Chinese market share between 2006 and 2010 – the world market of concentrated apple juice can be considered as a market with a moderate concentration. Based on the secondary research the study has a detailed examination of cause and effect relationships behind the RCA and HHI index results

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Comparative analysis of certain soil microbiological characteristics of the carbon cycle
137-141

In our researches, we examine the soil microbial parameters related to the carbon cycle. In this study, we compare the changes of microbial biomass carbon (MBC) and the soil CO2 production in soil samples which were taken in spring and autumn. The 30 years old long-term experiment of Debrecen-Látókép is continued in our experiment...s. The long-term fertilization experiment was set in 1983, and our sample was taken in spring 2014. The examinations of soil respiration processes and factors that influence soil respiration are required in optimal management. In our study, we interested to know how the growing levels of fertilization influence the soil respiration and microbial biomass carbon under non-irrigated and irrigated conditions in maize mono, bi, and triculture.

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Harvesting system established for the utilisation of Miscanthus sinensis ‘tatai’ “energy cane” in biomass power plants
143-150

The increasing demand for energy worldwide and the resulting environmental impacts of fossil fuels forced many countries to turn to renewable energy resources as a clean and sustainable alternative. More than a third of Europe’s binding renewable energy source target of 20% by 2020 will come from solid biomass for electricity and heating acco...rding to the National Renewable Energy Action Plans submitted by member states of the European Union (EU) to the European Commission. To achieve this goal long-term yield studies in renewable energy plants are important to determine mean annual biomass and energy yield, and CO2 emission. Field experiments worldwide and also in Europe have demonstrated that Miscanthus, a fast-growing C4 rhizomatous grass can produce some of the highest biomass and energy yield per hectare of all potential energy plants. Miscanthus is a plant that originates from the southern slopes of the Himalayas. It was bred for the Hungarian climatic conditions in 2006 under the name of Miscanthus sinensis ‘Tatai’ (MsT). The species has high frost and drought tolerance and high energy value. This is why there is growing demand for the biomass (lignocellulose) produced by growing this plant. The biomass, produced from the high yield energy reed, can be transported to power plants in large quantities, in forms of bales. Its household consumption is not yet significant. This study presents the external features, characteristics, propagation and plantation process of MsT energy reed. The study also demonstrates the harvest technology of the species worked out between 2009–2012 in Tata, Hungary and the options of supplying to biomass

power stations.

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The status of the Hungarian Merino sheep breed compared to the other merino breeds
151-156

Merino and Merino-derived sheep breeds have been widely known and distributed across the world, both as purebred and admixed populations. They represent a diverse genetic resource which over time has been used as the basis for the development of new breeds. In spite of this, their gene-pool potential is still unexplored. The Merino sheep repres...ent the most important sheep resource of the Hungarian husbandry. It has the largest amount of individuals between both of the stock and commercial flocks. But in Europe the Merino stocks went through a drastic reduction in number. Thus these breeds became endangered in several countries as well as in Hungary. In this study we would like to present the recent status of different Merino breeds of the world to ground our further phylogenetic research with the Hungarian Merino breed.

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The effect of genotype and the location of sampling on the mineral content of wool
157-160

Mineral supplementation is very important in high producing farm animals. The estimation of exact mineral intake is very difficult in forage eating animals, like sheep. Accessing of long term mineral status seems to be possible using wool mineral analysis. However several factors can affect the results. Therefore, the aim of this study was to t...est the effect of breed and sampling location on the mineral content of sheep wool. 20 Dorper and 20 Tsigai sheep were chosen from the same farm. Samples were obtained from 3 locations (withers, side and quarter) and tested for 8 elements: Ca, Mg, Na, Co, Cu, P, S, Se , Zn. The samples were cleaned with ethyl alcohol from organic contamination, then after adding nitric acid were mineral analized using ultrasonic cleaning unit. The samples were analysed with ICP-OES (Perkin-Elmer, Optima 3300 DV). Statistical analyses were carried out by GLM procedure of SAS statistical analyses software. Differences between means were checked with Tukey-test. Significant breed differences were detected in the case of Mg, Na, S, Se in spite of the same feeding regime. The wool mineral content were within the reference range. The sampling location had no effect on the mineral content of wool.

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Changing of antioxidant and sugar contents of self brewed beers during the brewing steps
161-166

Beer is a valuable beverage, its composition is influenced by raw materials and technology. We tried to determine how the technological steps influence the antioxidant content, the colur value, and the carbohydrate content, and how fruit syrups influence the antioxidant content and the colour value.

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Comparative analysis of sample preparation methods to determine the concentration of arsenic in soil- and plant-samples
167-170

Arsenic contamination of the fields and groundwater is a global problem. Alföld is the most affected area in Hungary. Irrigation witharsenic contaminated water, and crop production on the contaminated soil can cause a food safety problem, because arsenic is easy taken up by the cell of the plant roots. To prevent this, very important to monito...ring the arsenic content of soils and plants. Inductively coupled plasma mass spectrometry (ICP-MS) is a fast, easy method to determined the concentration of minerals in the case of plant and soil samples The analytical methods can give reliable, results if the analytical process, including the sample preparation method, is the best. The objective of this study was to compare 3 type of sample preparation method which was dry ashing, wet digestion in open system, and microwave digestion. As a result of our experiement shows the microwave digestion is the appropriate method to determined the arsenic content of soil samples. In the case of plant samples we can use wet digestion in open system or microwave digestion as a samle preparation method.

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