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Reproductive biology of Duranta repens L. (Verbenaceae) in relation to its environment
51-55.Views:218Impact of environmental changes (temperature and RH) on reproductive biology in Duranta repens (Verbenaceae) growing at ten different sites of Agra was studied. It flowers throughout the year, with optimum flowering in September. The flowers are arranged in loose clusters on terminal or axillary racemes. They are either blue or lavender in colour, hermaphrodite, actinomorphic and complete. The plants exhibit floral polymorphism (increase and decrease in number of petals and stamens) and considerable variation in extent of pollen fertility, floral density, insect pollinators and fruit-set percentage. The changes in temperature and relative humidity during the entire flowering period, was found associated with the variation in floral structure, pollen fertility and fruit-set percentage. Based on the percentage of fruit-set during different seasons of a year, there were three distinct periods, namely maximum, moderate and minimum periods. The present paper deals with the comparative view of reproductive biology of this ornamental plant in these periods. During the months of August—November when temperature ranges between 13.7-36.6 °C and RH between 79-89% the plant exhibits maximum fruit-set percentage (68-85%). This was associated with maximum flowering, increase in floral size, and increase in visitation rates of pollinators and higher degree of pollen fertility. On the other hand, with temperature reaching to the maximum (15.1-41.5 °C) and reduction in RH (14.1-41.3%), the percentage of fruit-set was reduced to the minimum (21-30%). During this period, number of flowers/plant, floral size, pollen fertility, visitation rates of pollinators were reduced to the minimum. During this period floral polymorphism was also 'recorded.
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Giant reed (Arundo donax L.) from ornamental plant to dedicated bioenergy species: review of economic prospects of biomass production and utilization
39-46.Views:483Giant reed (Arundo donax L.) is a perennial, herbaceous grass, it has been spread all over the world from continent to tropical conditions by human activities. In continental climate, especially Hungary, it has been considered as ornamental species, due to its decorative appearance, striped variants’ colour of leaves, long growing season and low maintenance requirements. It does not produced viable seeds, so it can be propagated vegetative ways by rhizomes or stem cuttings and by in vitro biotechnology methods. Because of its growth habits and good adaptation capability, it has been considered invasive weed primarily in coastal regions in warmer climate areas. In the previous century, giant reed produced for paper/cellulose/viscose production, woodwind musical instruments, stakes for plants or fishing rods etc. Over the last few decades, it has been produced for bioenergy purposes (bioethanol, biogas, direct combustion) or utilize as chemical basic compounds or construction materials. It has been considered a dedicated promising biomass crops thanks to high biomass production, high energy balance of cultivation and adaptability of different kind of soils and conditions. The objective of the present paper is to overview the most significance literature data on giant reed production and utilization, compare to own experimental data and economic calculations and to determine some critical factors, advantages and disadvantages of giant reed production compare to other biomass species.
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The main colouring substance and essential oil components of different carrot varieties
46-49.Views:157Investigating colouring components it was found that the p carotene being the most important from the point of view of nutrition constitutes about 60-70% of the total colouring material, whereas the ratio of a carotene is 18-34%.
Among the varieties having a short growing season the common incidence rate of the a and 13 carotene is very high (92.49 %), even a lower total colouring material content (178.02 ppm) results in bright orange red carrot roots.
The incidence of a and carotene represents, however, only 84.24% of the similar total colouring material value (171.74 ppm) measured in Nantais.
Among the storage varieties Fertődi vörös has the highest value of total colouring substance (213.04 ppm) from which the common proportion of the two carotene (a and (3) compounds amounts to 93.77%.
When testing the essential oil components the cariophyllen shows the highest incidence rate, which has a negative influence on the flavour materials of the carrot. Among the early varieties in Nantais Forto this quantity amounts to 24.08 ppm which is almost twice as much as the amount measured in Nantais. We found a similarly unfavourable quantity in %lords &ids (25.47 ppm).
Looking at the beta-pinen and 1-limonen of a bactericidal effect the quantity of l-limonen is higher (1.36 ppm on the average of the varieties). Among the varieties that have a longer growing season and which are suitable for processing Fertődi vörös is the best in terms of essential oil and colour content. Its beta-pinen content was above the average of this variety (1.12 ppm) while its 1-limonen content was considerably high (1.26 ppm).
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Commercial varieties of European plums grown in Hungary, a comparison of promising foreign varieties with the widely grown traditional ones
15-22.Views:253Observations and investigations performed in laboratory at several growing sites over a period of many years are summarised on established plum varieties as well as on new promising ones concerning their marketing value. The ripening period of plum varieties was enlarged substantially with the appearance of new varieties of extra late maturity (‘Elena’, ‘Tophit’, ‘Presenta’). The new varieties did not alter the traditional colour, more or less long, violet or blue character of the plum. The most known type, ‘Besztercei szilva’ with its accustomed taste was followed by the new varieties ‘Katinka’, ‘Tegera‘, ‘Hanita’ and ‘Presenta’. The fruit size are largely of the medium category, except the early ripening small ‘Katinka’, whereas the larger and attractive (~60 g) fruits are represented by the late ripening ‘Tophit’ and ‘Empress’. The stone/fruit ratio was lowest in 3% (‘Tophit’) and 6% (‘Besztercei Bt. 2’, ‘Hanita’, ‘Jojo’, ‘ ‘Čačanska rana’) at the other end of the scale. The width and thickness of the fruit fl esh between 28 mm (‘Besztercei szilva’) and 43-44 mm (‘Empress’, ‘Tophit’). The fi rmness of the fruit fl esh excelled in the late maturity varieties ‘Presenta’ and ‘Tophit’ (~4 kg/cm2). Water soluble solids were 12-13 Brix% (‘Čačanska rana’, ‘Katinka’, ‘Silvia’) and 20 Brix% (‘Presenta’, ‘Tophit’), whereas titrated acids are found between 0.2% (‘Besztercei Bt. 2’) and 1.2% (‘Tegera’).
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Test of the utility of apple retrotransposon insertion patterns for molecular identification of 'Jonathan' somatic mutants
7-10.Views:171Up until today, apple sport mutants proved to be indistinguishable from each other and their progenitors at the molecular level using random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) marker techniques. This is not surprising, since the genomes of these somatic mutants differ only in one or a few small regions that affect economically important characteristics, such as improved fruit colour, size, or flavour. In most cases, these genome differences are probably caused by retrotransposons which are able to convert their RNA transcripts to DNA with reverse transcriptase enzyme prior to reinsertion, but unable to leave the genome and infect other cells. Retrotransposon insertions can alter the expression of other genes and/or the structure of encoded proteins. The sequence-specific amplified polymorphism (S-SAP) technique is capable of revealing the genetic distribution of retrotransposable elements over the whole genome. The present study used this approach to try to characterize and distinguish 'Jonathan' somatic mutants via fingerprinting, which is an unsolved problem.
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Obtention of new ornamental leaf variants of giant reed (Arundo donax L.) originated from somatic embryogenesis and their photosynthetic parameters
18-24.Views:415Giant reed (Arundo donax L.) is a perennial rhizomatous herbaceous plant, it has been widespread all over the world by human activities. It is a 2-8 meter high, polyploid, sterile species (not produce viable seeds), it can be propagated only by vegetative methods (rhizomes, stem cuttings, in vitro biotechnological methods). It has considered promising dedicated energy crop thanks to high biomass production (20-40 dry tons per hectare depending on microclimate), adaptability of different kind of soils and environment and low energy input required for its cultivation. It has been utilized for energetic purposes, biogas/bioethanol production, cellulose/paper production and ornamental purposes. The objectives of the present study were to determine morphological properties of new ornamental giant reed leaf variants originated from somatic embryogenesis and to evaluate their photosynthetic pigment content and photosynthetic activities. The most typical changes was the appearance of different colour (white, yellow, light green) longitudinal stripes on leaves (also on petiole and on leaf blade). It was significant differences (p<0.05) between green and leaf variants in case of photosynthetic pigments content and photosynthetic activity (Fv/Fm value). There was no detectable chlorophyll a or b content in the white bands of leaves and albino shoots. Total chlorophyll content of the white striped leaf variety was more than twice than the light green leaves. Photosynthetic activity and content of photosynthetic pigments also confirm and determine the morphological characteristics and growth habit of leaf variants.
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Long term investigations of flowers and leaves on mainly non-domestica plums
73-79.Views:161The author dealt with plum species representing different eco-geographic areas by their genetic adaptation and their hybrids, as European (P. domestica, P. italica, P. cerasifera), Asian (P. salicina, P. simonii, P. ussuriensis), American (P. americana, P. besseyi, P. munsoniana, P. tomentosa). The rootstocks of the trees examined were seedlings of C. 679 myrobalan with the exception of Laroda and Santa Rosa II, which were grown on three different stocks: seedlings of C. 174 myrobalan, C. 449 bitter almond and C. 471 sweet almond. The size of peduncle, length of pistil, stamen number per flower, relative stamen number (SN/PL) have been suitable for description and distinction of varieties. Similarly shape of leaves, length of petiole, length and width of blade helped the identification.
The ratio of the dimensions of leaves, length of petiole and of leaf blade, also contributed to the distinction of European, Asian and American plum species, notwithstanding their relations with ecological conditions as well as historical, technical properties, pomological features, etc. Computed indicators (relative stamen number and shape-index of leaves) also have been useful data.
Significant correlations have been found between colour of nectaries and mean values of variety-groups. The potential values of non-European varieties for purposes of commercial production could be forecasted from the point of view of quality, ecological, pomological as well as market value. It is important, however, to know the effect of the rootstock and growing site as well as their interaction, on the one hand, whereas the resistance or tolerance of the varieties as limiting factors, at least to the sharka (Plum pox) virus, Xanthomonas pruni, on the other hand (cf. Surányi & Erdős, 2004a and 2004b).
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Concentration of ‘Oblachinska’ sour cherry juice using osmotic distillation
31-34.Views:230Sour cherry is one of the most famous and characteristic Hungarian fruit what is also commonly used in food production as a colour additive. The sour cherry cultivar, ‘Oblachinska’ was shown to be a rich source of antioxidant compounds, including mainly anthocyanins. Valuable compounds in fruit juice – vitamins, polyphyenols etc. –are heat-sensitive molecules, which should be taken into account during the process of concentration to prevent degradation. Osmotic distillation seems a suitable option to product high quality sour cherry juice because this process does not require high temperature or pressure. Raw juice with approximately 15°Brix was used for the experiment and tried to concentrate up to 60° Brix where the water activity low enough to inhibit the microbiological deterioration. Before and after the process, TPC (total phenolic compounds) and FRAP (ferric reducing antioxidant power) was measured using spectrophotometric methods to determine the effect of the osmotic distillation. Our results point out that osmotic distillation is a promising method to concentrate sour cherry juice and prevent the loss of valuable compounds.
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Atropine and scopolamine in leaf and flower of Datura arborea L.
61-64.Views:224Angel's trumpet (Danira = Brugmansia arhorea) is a common, popular ornamental plant in Hungary. On the basis of thin layer chromatographic and densitometric alkaloid studies of leaf and flower samples collected from several places in Somogy and Baranya counties it can be stated that in samples of cultivars with different flower colour, taken approximately at the same time, the atropine and scopolamine content varies: there are samples free of alkaloids, but most of them are rich in alkaloids. Although the means do not reflect the extremes, they are suitable for determining the alkaloid accumulating capacity of flowers. Generally the amount of atropine and scopolamine is significant both in the leaf and the flower. It is conspicuous that the flower can be characterised by an extremely high scopolamine content (mean in mg/g dry matter of leaf: atropine 0.34, scopolamine 0.31; of flower: atropine 0.26, scopolamine 0.85). Thus it can be stated that the leaf, and especially the flower of angel's trumpet is a potential hallucinogen, just like in the case of Datura stramonium.
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Anatomical relations of root formation in strawberry
71-75.Views:158Anatomical relations of root formation are traced throughout the life cycle of the strawberry plant from the germinating seed up to the runners of the adult plant. Histological picture of the root changes a lot during the development of the plant. First the radicle of the germ grows to a main root, which makes branches into side roots and later adventitious roots are formed on the growing rootstock or rhizome. The anatomy of the different types of roots is also conspicuously different. First tiny branches appear relatively early after germination on the seedling's radicle, but soon the hypocotyl of the seedling thickens and develops side roots, which are already somewhat stronger. During this interval, the first true leaves are formed. The 4th or 5th of them being already tripartite, and the initiation of new roots extends into the epicotylar region of the shoot. The second years growth starts with the development of reproductive structures, inflorescences and runners starting from the axils of the new leaves. Near the tips of the runners below the small bunch of leaves, new root primordia are initiated. The tiny radicle of the germ develops a cortical region of 5-6 cell layers. Cells of the central cylinder are even smaller than the cortical parenchyma and include 3-4 xylem and 3-4 phloem elements as representatives of the conductive tissue. Roots originating from the shoot region are much more developed; their cortical zone contains 17-20 cell layers, whereas the central cylinder is about half as large. In the next year, new roots are formed at the base of the older leaves. These roots differ hardly from those of the last season in size and volume, however, they are recognised by colour and their position on the rhizome. The roots of the last year are dark, greyish-black, and grow on the lower third length of the rhizome, on the contrary, the new ones, on the upper region, are light brown. Roots starting from the shoot or rhizome are, independently from their age or sequence, mainly rather similar in size and diameter, thus being members of a homogenous root (homorhizous) system, i.e. without a main root. Plants developed and attained the reproductive phase develop in the axils of the leaves runners being plagiotropic, i.e. growing horizontally on the surface of the soil. The runners elongate intensely, become 150-200 mm, where some long internodes bear a bunch of small leaves and root primordia on short internodes and a growing tip. Runners do not stop growing, generally, further sections of 15-25 cm length are developed according to the same pattern, with small leaves on the tip. The growing tip of the runners is obliquely oriented, and small, conical root primordia are ready to start growing as soon as they touch the soil. The roots penetrate the soil, quickly, and pull, by contraction, the axis of the runner downwards, vertically, developing a new rhizome. The short internodes elongate a little and start developing adventitious roots. At the end of the growing season, the plantlets arisen on the rooted nods of runners are already similar to the original plants with homogenous root system. On the side of the adventitious roots, new branches (side-roots) are formed. The root-branches are thinner but their capillary zone is more developed being more active in uptake of water and nutrients. The usual thickening ensues later.
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Evaluation of elder (Sambucus nigra) varieties and candidates for the canning industry. Results of the composition studies
102-107.Views:152Sambucus nigra is a very common elder species in Europe. Due to its excellent composition, natural dye content and healing power it can be considered as a biological active plant. In Hungary the cultivation of Sambucus nigra started in the nineties and since that time there is a growing demand for this plant. Sambucus nigra is a special fruit due to its sole.-application for processing industry. Partly fruit products are made of it, partly it is used as a natural dye. In both cases the first step is the knowledge and evaluation of the composition of the various elder varieties and candidates. For that reason composition studies of different elder types were performed in several years at the Department of the Canning Technology of the Szent Istvan University.
The most important sample was the Haschberg variety, which is the sole elder type accepted and allowed for propagation by the government. This plant is grown in the Pilot Plant of the Szent István University in Szigetcsép. Among the studied samples there were some varieties cultivated in the Fertőd Research Station. They were the following types: No. 33, 480 and 481. Besides elder collected wild in Szigetcsep and the very promising early-ripened SZ-CS 21-23 variety originated also from Szigetcsép were investigated. One year it was possible to study the Sambu variety, too. Department of the Fruit Culture of the Szent István University helped to obtain the various elder types and candidates. Along our experimental activities, the following components and parameters were studied: the content of pectin, organic acid, carbohydrate, mineral, vitamin C, total acid, aquesoluble dry material, anthocyanin and the pH.
After having obtained a juice, a concentrate from the species has been made and — after freezing — the aquesoluble dry substance and the acid content, as well as the colour parameters were monitored. Studies were performed in two years (1997-98). On the basis of these results classes were established for all compounds, from which an overall ranking was derived.
It was found that in both years the Haschberg variety proved to have the best composition. Therefore regarding to the overall series of order the experiments performed in the second year supported the results obtained in the previous year. Results have shown that Sambucus nigra has excellent composition beside its curative effect. Its mineral content has surpassed that of the other fruits and it had advantageous acid-and carbohydrate composition. The ascorbic acid content of the Haschberg variety is striking. It can be concluded from the experiments that the Haschberg type has the most advantageous composition, therefore it is suitable for making special curative and exclusive products.
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Microclimatic studies on different aged apple plantations
7-11.Views:188The purpose of measuring parallel canopy and out of canopy microclimates was to find out in what extent climatic parameters measured in different aged canopis differ from each other and from the values characteristic to out-of-canopi areas. The importance of phytoclimatic researches seems to lie in the fact that if the reactions of fruit trees towards meteorological elements are continuously followed, we have the possibility to provide growers with information. These pieces of information are like defining the optimum time of phitotechnical interventions (summer pruning, sorting sprouts, thinning fruits, etc.), the necessity of applying mulching, defining the method and time of irrigation and applying plant protection activities. By means of phytoclimatic researches, it is possible to react to unfavourable meteorological impacts within a certain extent. It is also possible to successfully reduce the risks of late spring and early autumn frost damage, as well as the risks, content and measure of experienced heat and water stress conditions by finding out about the physical characteristics of the canopis' internal area.
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Comparison of Xanthomonas arboricola pv. juglandis isolates from walnut trees grown in Romania and Hungary
65-69.Views:262Among diseases that affect walnut, bacterial blight is considered the most important one in all walnut growing areas both from Hungary and Romania. For the determination of susceptibility/resistance of walnut cultivars in our posterior work planned, 61 bacterial isolates were collected from walnuts showing symptoms of blight, with the purpose of isolating Xanthomonas arboricola pv. juglandis (Xaj). The characteristic Xaj colonies, frequently present as saprophytes in the infected plant tissues, were separated from other bacteria according to their morphology, yellow colour, hydrolysis of starch, and oxidation of glucose. All isolates were tested for pathogenicity by hypersensitive reaction on tobacco leaves (Nicotiana tabacum L.), bean pods (Phaseolus vulgaris L) and unripe nuts (Juglans regia L.). Determination of taxonomy of the selected isolates denotes a possible subdivision (races, biotypes) of Xaj occurring in different geographical areas, API 20NE and API 50CH kits were used. Hungarian and Romanian strains showed a high degree of similarity of carbohydrate utilization but slightly differed from the type strain. All were Cu-sensitive.
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Rootstocks for Cherries from the Department of Fruit Science Budapest
63-66.Views:161Cherry rootstock breeding started at the Department of Fruit Science, SSU Budapest by the late 50-s and the activity can be divided into three main groups. In the first stage the activity was focused on collection of native mahaleb cherry (Prunus mahaleb L.) varieties lead by L. Sebők. After evaluation in the nursery and orchard tests there are four promising rootstock cultivars selected from this material: 'Korponay' used as self fertile seed tree, its seedlings are recommended for sour cherries. The mahaleb varieties 'Bogdány' (vigorous), 'Egervár' and 'Magyar' (medium vigour) are propagated by cuttings. The next project has started in 1979 with the aim to select self fertile mahaleb seed trees producing homogeneous seedling populations with reduced vigour. Inbred populations from isolated flowering self fertile trees were produced and planted out in 1980. The inbreeding of 'Korponay' self fertile P. mahaleb variety resulted in specimens with different fruit colour (yellow, red, black), fruit shape and size. From among them self fertile trees were selected with various growth characteristics. Seedlings of that self fertile mother trees (S2 population) were tested in seedbed, they showed homogeneous phenotype characteristics as liners in the nursery. As rootstock of 'Érdi bőtermő' sour cherry in the orchard most of the S2 lines proved to be less vigorous in comparison to SI popuplations. 'Érdi bőtermő' trees budded on certain S2 lines in the orchard are more productive than those on S1 ('Korponay' seedling). Characteristics of the S2 generation as seed tree were studied as well. We expect to get morphologically homogeneous seedling populations with different growth vigour and good productivity in the later inbred generations. In the last couple of decades the research activity concerning ground-cherry and its hybrids resulted in dwarfing rootstocks. Prunus fruticosa Pall. hybrids from the natural flora of Hungary were collected and artificial hybrids were created between P. fruticosa and mahaleb cherry. Most of them are in the initial tests, only one of them is before registration, named 'Prob', which is a dwarf rootstock for sweet cherry. By the screening of new hybrids medium vigorous or semi dwarfing and precocious rootstocks seem to be promising for the cherry industry.
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Response of sour cherry cultivar `Érdi jubileum' fruits to modified atmosphere packaging after ethephon spraying
81-85.Views:167Sour cherries are not adapted to long storage. The largest percentage of sour cherries reaches the consumer either directly or indirectly through the tin can or the frozen pack. Different concentrations of ethephon usually are used commercially to facilitate harvesting sour cherry fruits that this hormone influences on fruit quality. The objective of this study was effect of ethephon preharvest application on sour cherry fruits quality during storage period at modified atmosphere packaging was investigated. Modified atmosphere packaging used with 10, 15 and 75 percent for 02, CO, and N2 respectively that those held at 0 °C. This gas compounds synchronous to 0 °C temperature caused to increase shelf life postharvest of fruits. Fruit samples were evaluated at harvest date and after 6 weeks in storage. Skin fruit colour, pH, total soluble solids, titrateable acidity, sugar/acid ratio, firmness were monitored. Ethephon concentration influenced on total soluble solid, titrateable acidity, sugar/acid ratio and L* value. In more case, 225 ppm ethephon's concentration has no observable different to control samples. Thus, the best ethephon's concentration was 225 ppm ethephon's concentration.
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Alternate bearing of 'Golden Reinders' and 'Summered' apples
77-85.Views:203The aim of the present study was to study the effect of biennial bearing (irregular yields) on the generative production of apple cultivars 'Golden Reinders' and 'Summerred' on M.9 rootstock. The observations were made at Nagykutas in West-Hungary for four years. The authors have studied the flowering time, flower density, fruit density, fruit drop, seed content, yielding and fruit quality in the on-year and off-year. Based on the results, it can be stated that the alternation does not cause a significant change in the flowering time of cvs. 'Golden Reinders' and `Summerred': the difference between the flowering time of trees in the on-year and off-year period was 0-1 day. However, there were significant differences in the flower density. 'Golden Reinders' proved to have a weaker tendency to alternation similarly to the statements of the literature. Alternation (off-year) was observed only in three years from the studied four years. While in the case of `Summerred', strong alternation was observed in all four years. In fruit density, there were differences among the cultivars and among the different cycles of biennial bearing. Fruit density (and its opposite, fruit drop) changed inconsistently in the period between flowering and harvest. In the case of 'Golden Reinders' and 'Summerred' 2 and 4 fruit drop periods were detected, respectively. In the off-year, the different periods were less distinct, in most cases they were overlapping each other. The dynamics of fruit drop was related to seed content per fruit. The lower the seed number was, the higher the degree of fruit drop was. In both the high- and off-year cycles, the number of seeds in fruits on the tree and on the ground increased with time. In the yield parameters (fruit number, fruit load, yield efficiency), the differences were greater among the yields of trees in the high- and off-year cycles for 'Summerred'. The fruit quality parameters were greatly influenced by the fruit load of the trees in the different alternation cycles. In general, it can be stated that fruit mass, diameter and height were lower on trees in the on-year. A similar trend could be observed in flesh firmness, cover colour and dry matter content, but the differences were smaller. 'Summerred' was more sensitive to the differences in fruit load.
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Colour and water content detection of sweet cherry by portable spectrometer
23-26.Views:295Based on the most recent data, the average amount of sweet cherry produced in Hungary is around 10-12 thousand tons. Therefore fast and effective method is important for sweet cherry fruit quality analyses. The aim of the study was to examine the applicability of reflectance measurements for sweet cherry fruit quality analyses. In our experiment five cherry species (Vera, Cristalina, Germersdorfi, Noir de Mechet, Canada Giant) were examined in order to measure the spectral differences between species. Further more, spectral alteration was examined between different health and maturity status of the fruits in the case of a specified, the Germesdorfi species. The four new indices are appropriate tools for cherry quality analysis. Thus reflectance measurements can also support more precise and automated fruit selections. The methods for the differentiation of species could also be viable at a concerned habitat; however, the climate, habitat and soil conditions strongly affect the yield quality. Concerning the fast determination of water content, WBI could be a reliable method for the assessment
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Bean tissue culture and genetic transformation with Agrobacterium
32-35.Views:117In this paper we report the establishment methods of a rapidly growing callus culture of Phaseolus vulgaris bean as well as the conditions required for a high level of transient gene expression using Agrobacterium-mediated transformation. A vector is containing both the lindan-resistance gene as a selectable marker, and GUS gene as a screenable marker. By using hypocotyl explant and vertical culture on B5 medium supplemented with 1 mg/1 kinetin- and 2,4-D 2 mg/1 and subcultured every 3-4 weeks, we can recommend to get a good and much callus from bean. This will help in introducing foreign DNA into callus cells. One strain of Agrobacterium carrying plasmid as vector for introducing foreign DNA into plant cells was used. At different concentrations of lindan; 3, 4 and 4.5 mg/I, the transformed Maxidor callus survived and grew over a period of 6 month and subcultured every 3-4 weeks, but the control callus died. Callus were assayed for GUS activity to confirm the expression of the GUS gene using the histochemical assay test. The GUS gene was also correctly expressed in callus cultures grown on 4mg/I lindan-selected medium, the typical blue colour in the histochemical assay using the X-gluc as substrate. But the control, non-transformed callus was not able to grow in the presence of lindan, neither showed a positive reaction in the in vitro assays.
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Comparative study of heirloom tomato varieties
31-33.Views:188The study examined the health status, growth type, berry morphology, berry quality, and gastronomical value of 60 heirloom tomato lines. In the middle of June, the level of virus infection was medium in the plant stock according to the assessments. 21% of the lines did not show symptoms of virus infection. During the vegetation period, the major fungal diseases were Alternaria by the middle of August and Phytophthora from the beginning of September. In spite of the fact that no fungicides were applied, 13% of the lines were healthy and 36% showed medium infection at the end of August. Half of the lines had a strong, 23% of the lines had a medium growth vigour. The majority (74%) of the lines showed strong or medium lateral shoot growth and only 7% of them proved to be less prone to branching. In spite of the rapid growth, 20% of the lines had a weak main stem, the majority of the lines had a main stem medium strength. The different shapes occurred in different colours, in addition to the traditional red colour (53%), there were orange, yellow, wine-red (blackish), lilac, green, almost white or multicoloured varieties. Unfortunately, most of the lines were prone to longitudinal or circular cracking of the berries. Based on the first experiences, all tested variety types had more favourable utilization characteristics then the traditional tomatoes. Varieties with a thick flesh and harmonic acid/sugar ratio such as some of the ox-heart varieties are especially valuable. For drying, the elongated types proved to be the best. The hollow tomato is the most special type which can be utilized most diversely. It is especially suitable for making white tomato soup and, “Concasse" due to its easily removable placenta rich in jelly materials and its thick flesh. In addition, it can be used for preparing stuffed tomato.
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Relation between the germination and infection ratio on Sida hermaphrodita L. Rusby seeds under hot water treatment
117-121.Views:173Sida hermephrodita or virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through between ten and twenty years. Additionally, the plants have a lot of uses and benefits for instance it can use it as a fodder crop, honey crop, ornamental plant in public gardens. It has favorable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in base stage of domestication therefore has a serious disadvantage the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seed/acre instead of 10-20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a disposable ,basically wild Sida population. We examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenus or endogenus infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65, 80 and 95 oC), 29,33 to 46% germinated of the seeds collected from the population of S. hermaphrodita in Debrecen. The average germination for all season was 5-10 % wihitout treatment and rised using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46 %) after the hot water operation in spite of the previus studys (Spooner 1985; Chudik et al. 2010; Doliński R. 2009.). We discovered that there are a distinguished close relationship between the seeds collecting time and the infection, as well as germination percentage. Thus, 2009 season was the most favourable in case of contamination (control:17,33 and 80 oC treatment:0%) as well as germination percent. It could be concluded that, the best season for our findings was 2009 due to autumn harvest of Sida seeds. In our oppinion, the autumn harvesting should be the best time to overcome the problem of the low germination and high infection percentage.
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Fruit Quality of Sweet Cherry Cultivars Grafted on Four Different Rootstocks
59-62.Views:175A rootstock trial planted at the Szigetcsep experimental station in 1989 involved the study of two cultivars- `Germersdorfi FL 45' and 'Van' -grafted on four different rootstocks — 'Colt', `MxM 14 — Brokforest', `MxM 97 — Brokgrow' and 'Saint Lucie 64' as a control. The trees were trained to the "Modified Brunner-spindle" system and came into bearing in 1993. The yield per tree, fruit weight and fruit diameter have been measured each year since then. The refractivity, the acid content of the fruit juice, fruit cracking after four hours' dipping in water and stone weight ratio have also been measured since 1995. In 1997 and 1998 these parameters as well as fruit cracking after 24 hours' dipping were measured. Fruit firmness and fruit colour were also estimated. In almost every observed parameter significant differences were found between the scion cultivars. Yield efficiency was significantly higher on 'NUM 14' and 'Saint Lucie 64' than on the other two rootstocks. As regards fruit weight, in both cultivars and over an average of six years, it was found that trees on low yielding tree on 'Colt' rootstock had the highest fruit weight values and on heavy producing `MxM 14' the smallest. Soluble solids content was higher on 'Colt' and `MxM 97'. No significant differences between the rootstocks were found in acid content of the fruit juice. There were significant differences between the rootstocks in fruit cracking after 4 and 24 hours' dipping in water. Seemingly with respect to cultivars and rootstocks the year has a considerable effect on fruit cracking.
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Examination of fruit juices produced with aseptic technology
65-70.Views:134The quality of finished products with a 100% fruit content is influenced to the greatest extent by the fruit-base (fruit pulp and fruit juice concentrate) used. In terms of the production of the fruit juice, the minimization of the quantity of the dissolved oxygen, which can cause detrimental oxidation processes to start, is a very important aspect. Scientific experiments specify de-aeration before pasteurization as the solution to the problem. The choice of the packaging material and the manner of packaging also contribute to the preservation of the alimentation-biological and pleasure value of fruit juice. On the basis of the results of the experiments, regarding the parameters which are important in terms of quality, i.e. dissolved oxygen content, C-vitamin concentration, deepening in the colour and sensory features, Tetra Bric Aseptic packaging proved to be better in the examined cases. Storage circumstances also influence the quality of fruit juice.