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Assessment of soil characteristics in orchard
11-14.Views:1278The research field was at Siófok, in Hungary, which is situated in the South East side of Lake Balaton. The physical characteristic of the soil is sandy loam and loam and the peach orchard is irrigated. The detailed goals were mapping and analyzing of physical properties of the soil in water management point of view, mapping the acidity and CaCO3content of soil for precision liming, measurement of humus the element content. Sites with different physical characteristics (from sandy loam to loamy clay) could be distinguished. The reason for this is that besides the possible increase of clayminerals, the increasing rate of colloidal humus content contributes to larger soil plasticity. Statistics also proved positive and strong correlation (r=0.822) between the soil plasticity and humus content. In the case of pH, only a small part of the orchard is has to be limed, since most of the orchard has neutral pH, which is advantageous for nuts and stone fruits. It has to be mentioned, that the CaCO3 supply is also appropriate for the stone fruits. Based on the results hyperspectral imagery can be a good solution for detecting calciferous soils, although these measurements are still need validation.
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Illumination-Independent Reflectance Information Acquirement for Leaf Water Potential Measurement on the Example of Satsuma Mandarin
75-79.Views:232Mandarin fruit sugar content can be increased when subjecting the satsuma mandarin tree (Citrus unshiu MARC. var. satsuma) to moderate water stress by mulching during the period of active sugar accumulation, thereby fruit quality improvement can be realized. In the frame of the precision agriculture production system, a non-destructive measuring method development became necessary based on remote sensing, field spectroscopy and image analysis, to be able to measure the degree of water stress. Large amount of visual information have been recorded at ground level, in near infrared, red and green channels by a false color digital camcorder designed specially for remote sensing applications. A method have been found to be able to calculate the absolute reflection of mandarin leaf surface by comparing leaf reflectance with known reference target reflectance, thereby established the basis of further studies in this topic. Leaf absolute reflection can be measured reliably, under variable natural illumination at field conditions. Functional correlation can be searched between visual information and leaf water potential measured by PMS pressure chamber.
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Spectral properties of strawberry plants
17-22.Views:603Strawberry varieties were studied in 3 different growing areas of different soil- and ecological properties in Hungary. We have measured the optical spectra of the leaves together with their nutrient content and performed sensory examinations on the deep frozen and melted fruit. The 6 varieties were Elsanta, Marianna, Spadeka, Symphony, Camarosa, and Raurica. The 3 sites were Újfehértó, Pölöske, and Kecskemet. Colour of the leaves was characterised by their lightness and dominant wavelength. Under non-optimal soil and ecological conditions the varieties could be distinguished by their colour — the leaves became lighter and more yellow. The fruits did not produce the genetically determined taste if the plant can not grow in optimal circumstances — we found a negative correlation between the leaf colour in flowering time and the fruit flavour. Leaf colour measurements performed in flowering time make it possible to improve fruit quality by changing the nutrient supply between flowering and ripening.
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Pollen morphology of fruit species
49-57.Views:17388Size and surface morphology of pollen has been studied in 87 twit varieties of 10 fruit species during the period of 1990-1995. No preceding work of that type came to our knowledge, yet.
The samples comprised a wide variety of cultivars included male sterile, self-incompatible, partially self-fertile stone fruits, diploid and hexaploid plums, diploid and triploid apples.
The large number of species and varieties facilitated the comparison of items within and between the respective species.
It was stated that the size, shape and surface morphology of pollen is genetically determined and those data, combined with other variety characters, are suitable for the classification and distinction of varieties.
In assessment of pollen size and shape, their moisture content is crucial. The major diameter of the swollen pollen as well as the length and width of the dry grains are characteristic to species and/or to variety.
The width and shape changes largely with moisture content. Large grains are proper to quince, apricot, peach and almond, medium sizes are found in apple, sweet cherry, sour cherry, European plum, whereas small size is typical to Japanese plums.
The low number of varieties studied does not allow conclusions concerning differences within pears, quinces and almonds as species. In the rest of species, valid differences have been registered as between varieties.
Within species, as apple and plum, the effect of ploidy (i.e. number of chromosomes) was expressed in the size of their pollen. In stone fruit species, the correlation between size. of anthers and size of pollen grains was positive.
Genetic relations between the self-fertile sour cherry varieties of the Pándy type (Debreceni bőtermő, Kántorjánosi, Újfehértói fürtös) as well as the self-incompatible apricots of "giant" fruit size are supposed to be analysed by pollen studies but there did not turn out any decisive conclusion, yet. Other characters also should be considered.
The assembly of pollen characters is decisive in the determination of the variety. The ratio of empty pollen grains, the grain size and the density as well as the size of the pits on the surface are best suited to distinguish pollen lots.
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Evaluation of morphological parameters and bioactive compounds in different varieties of beetroot (Beta vulgaris L. ssp. esculenta GURKE var. rubra L.)
31-35.Views:882Beetroot consumption based on pickled beets generally in Hungary which is due to the higher yield from second crop harvested in autumn and processed by manufacturing industry. Researches of the past years confirmed its favourable nutritional-physiological effects on human body so demands, as for fresh salad, also increased. The trial aimed at testing the interaction of varieties on quality parameters and in the same time suggestions are made how to use different varieties of beetroot according to its quality. Morphological and sensory evaluations were examined on 10 varieties of beetroot harvested in autumn. The regular spherical shape can reduce the refining loss during the processing of beetroot which is beneficial for the manufacturing industry. In our trial the root shape of Libero, Mona Lisa and Rubin varieties approached most the regular spherical shape (diameter/length – 1.0) which is favoured by not only processing industry but also fresh market. The highest red pigment content (betanin) was observed in Mona Lisa, Akela and Cylindra (34.58–47.66 mg/100 g). A similar trend could be observed in yellow pigments (vulgaxanthins) which proves the close correlation between the quantities of the two pigments (r=0.898). Highest total polyphenol (77.13–83.37 mg GAE/100g) and flavonoid (21.73–22.73 mg CE/100g) contents were detected in Akela, Mona Lisa and Bonel. These varieties are favourable for fresh salad and they can satisfy processing requirements also. Highest water soluble solids content was found in Akela (7.15%). In our conditions nitrate (NO3-N) values below 900 mg/kg were examined in all of the varieties which is favourable in the case of beetroot.
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Prediction infection risk on the basis of weather-related factors and Erwinia amylovora colonization in apple and pear flowers
39-54.Views:341Current infection risk prediction models utilize environmental parameters and field records, but do not take into account the estimated inoculum potential within the orchard. The object of this study was to survey the accuracy of three simple prediction methods under Hungarian climatic conditions, which could easily be used by the farmers. We also tested whether the accuracy of infection risk predictions can be improved by taking into consideration the incidence and/or rate of flower colonization by Erwinia amylovora.
After preliminary investigations in 1999-2001, data concerning the weather-related infection risk were recorded in 5 apple and 1 pear orchards in 2002, and in 12 apple and I pear orchards in 2003. The weather data were processed by the easy-to-use risk assessment models of the mean temperature prediction line (MTL), Smith's Cougarblight 98C and Billing's integrated system (BIS), and by the MaryblytTM 4.3 computer-assisted model for reference. The population size of E. amylovora in the flower samples was estimated within an order of magnitude by PCR.
For all years and orchards tested, Maryblyt indicated 35 days on which there was an acute infection risk. The same days were indicated by all 3 methods in 23 cases (66%), 8 days were indicated by 2 methods (23%) and 4 days were indicated by 1 method only. A similarly good correlation was found for prediction of the date of the first massive infection risk: in 2003, for instance, there was a perfectly consistent prediction by all 4 models in 9 of the 13 participating orchards. A coincidental forecast was provided by 3 of the 4 models in the other 4 orchards.
The results indicate that any of the risk assessment models could provide an increased accuracy of the actual infection risk prediction if combined with an estimation of the incidence of Erwinia amylovora colonization in the open flowers. We found no convincing differences in the size of the epiphytic population in flowers of cultivars possessing high or low susceptibility to Erwinia amylovora.
We conclude that the easy-to-use methods tested could be used by the fanners to recognize weather-related risks, especially when coupled with an estimation of the proportion of the pathogen-infested flowers. This local prediction would provide rapid information (faster than the regional forecast systems) specifically for a given orchard.
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Effects of aromatic cytokinins on structural characteristics of leaves and their post- effects on subsequent shoot regeneration from in vitro apple leaves of 'Royal Gala'
41-46.Views:465The effects of different aromatic cytokinins applied in different concentrations and combinations were investigated on the histology of in vitro apple leaves and their post-effects on subsequent shoot regeneration from these leaves were studied. Great differences in the anatomical structure of leaves could be detected originating from media containing different types and concentrations of aromatic cytokinins. The number of regenerated shoots per explant and the organogenetic index were used for the evaluation of the post-effect of aromatic cytokinins on shoot regeneration. The histological structure of leaves used for regeneration and their regeneration response showed a good correlation. When the pre-treatment caused a juvenile-like or less-differentiated structure, the number of regenerated shoots per explant increased and often vitrification also decreased and consequently the organogenetic index also increased. A strong interaction between cytokinin-content (type and concentration) of the pre-treatment medium and that of the regeneration medium could also be detected.
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Comparative study of different fertile groups in plums
71-76.Views:391The plum traditional fruit species in Hungary, several local cultivars was born in the different grower's districts. The author that investigated, that are morphological differences between an odds find self-fertile, self sterile (with functional stamens) and male sterile plum cultivars. For it thought about main questions of a scientific debate sown up and the study this way gave reactions totalize. There were in three fertile groups 8-8 type of feature plum cultivars in periods of 1992-2001, respectively 1993-1999. It was founded by author big odds found the troops on the basis of 9 traits between. Pistil length of self sterile cultivars very typical, such as sesquipedalian flower peduncle of the self-fertile plums and the hypoandry of male sterile cultivars. The relative stamen number and the pollen viability as well significant odds gave.
The average fruit mass and sharka infection of self-fertile plums this troops extreme work. According to cultivar's averages the shark symptoms standard the right correlation the singular traits, but those one part of her with each other not shown connection.
The annuity potencies underdeveloped the troops behind and the troops within cultivar's differences had case significance. The results usable the male sterile cultivars and progeny further its investments.
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The morphology of stigmata in stone fruit species
45-48.Views:976The morphology of the stigma has been studied in 50 varieties belonging to 6 stone fruit species. The majority of samples had elliptical stigmata with some exceptions with circular form (Duane, Tuleu gras). The surface of the stigma is papillary, flattened in side view (sweet cherry) or bulging (apricot, peach). The suture of the stigma is clearly visible as a depression and the varieties may differ in this respect.
The size of the stigma depends highly from the season, although the varietal differences are maintained. The dimension of stigmatic surface is characteristic for the species expressed in square millimetres: sweet cherry 0.92 to 2.91; sour cherry 1.64 to 2.48; plum 0.83 to 1.80; oriental plum 0.53 to 1.15; apricot 0.57 to 1.69 mm2.
The size and morphology of the stigma changes according to varieties too, and it may used in description and identification of varieties. No correlation has been found between the size of stigma and the fertility relations (self-fertility or self-incompatibility) of the respective varieties.
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The effect of different cytokinins on chlorophyll content and morphological features of in vitro Nidularium ’Kertész Jubileum’
47-51.Views:430During in vitro multiplication of Nidularium ‘Kertész Jubileum’, 20 g/l sucrose, 5 g/l agar, 100 mg/l inositol, and different concentrations of benzyladenine (BA), benzyladenine-riboside (BAR), kinetin (KIN), meta-topolin (mT) were added to the MKC (Knudson, 1946) basal medium. Furthermore, 0.1 mg/l naphthaleneacetic acid was used to every medium. Number of shoots, length of leaves, number and length of roots, chlorophyll (a+b) content were examined and evaluated with Ropstat statistical software. As compared to the other cytokinin, significantly most shoots were obtained in the case of applying BA. Increasing of BA-concentration (as far as 2 mg/l) enhanced shoot number (from 10.92 to 19.26) but 4 mg/l BA resulted only 6.63 shoot. The less efficient cytokinin was KIN, in most cases no more than about 2 shoot was achieved. Regarding the length of leaves, the higher level of BA effected averagely the shorter leaves (from 24,46 to 7.31 mm). KIN effected significantly the longest leaves (43.4-61.29) in inverse proportion to the concentration. The same cytokinin resulted the most (and the longest) roots with the highest rooting percentages, but more KIN decreased the number and length of roots (from 7.95 to 4.4 and from 38.49 to 22.73 mm). There were no definite correlation between cytokinin concentration and chlorophyll (a+b) content, but the highest doses resulted decreasing (except of meta-topolin which leads to the lowest values). Summarizing, BAR effected the highest contents (mostly more than 1400 μg/g), particularly in the case of 1 mg/l (1807.3 μg/g).
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Carbohydrate utilization of Erwinia amylovora in vitro
31-34.Views:406Nectar is a multi-component aqueous solution that promotes bacterial multiplication. The concentration of nectar in plant flowers is not stable since it is under the influence of environmental conditions, especially free moisture and relative humidity. Experiments were conducted with "artificial nectar" and directed along two lines: (1) determination of the optimal concentrations of carbohydrates for the growth of E. amylovora development (2) consumption of different carbohydrates besides basic sugars.
Solutions of "artificial nectar" were prepared in different compositions by changing the dominance of basic sugars (fructose — glucose —sucrose) in proportions of 2:1:1, 1:2:1, 1:1:2 and between concentrations of 10-0.6% (diluted with Basal minimum broth) in order to determine optimal conditions for the development of E. amylovora.
At a basic sugar concentration of 10% bacterial multiplication started and continued until I log degree (from 106 to 107 cfu/ml). At concentrations of 5% and 2,5 % cells developed with nearly the same kinetics (from 106 to 8x107 cfu/ml and from 106 to 9x107 cfu/ml, respectively). Multiplication was more pronounced and nearly the same at concentrations of 1.2 % and 0.6 % (from106 to 2x108 cfu/ml). At a basic sugar concentration 30% total sugars bacterial multiplication did not occur, while at 20 % it was negligible, not measurable photometrically.
At minimal concentrations of F, G, S (between 1-0.1 %) bacterial cells were still able to multiply, producing organic acids from sugars.
Our study showed that E. amylovora requires only a small amount of sugars (0.1%) for multiplication (acid production) while high concentrations inhibit multiplication. There was a negative correlation between sugar content and cell density. The optimal range of sugar concentration was at about 1%.
Effect of "less frequent carbohydrates" to E. amylovora multiplication was also determined using the API 50 CH strip. We could provide information on utilization of 39 carbohydrates by the bacterium at different categories as follows: Not utilized-, Slowly and weakly utilized-, Slowly and completely utilized-, Quickly and completely utilized carbohydrates. We suppose that carbohydrates that belong to the latter two groups could play an important role as nectar components in promoting E. amylovora multiplication in the blossoms of pome fruit trees.
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The application of A HEAT SUM MODEL for the budburst of sour cherry varieties grown at Újfehértó
105-112.Views:1009Experiences of the last decades showed univocally that the climatic changes, especially the warming up, influenced clearly the phenology, i.e. speed of growth and development of plants. To check the effects, the phenological studies became a topic of special interest. Our research has been performed at Újfehértó, the Research Institute of Fruit Growing and Extension, where the respective database accumulated observations during the period 1984–2005, where the meteorological data as well as the parallel phenological diary referring to the varieties ’Újfehértói fürtös’, ’Kántorjánosi’ and ’Debreceni bôtermô’ during the period 1984–1991 have been utilised. The method of calculating the sum of daily mean temperatures, “degree days”, is based on the observation that the plants are able to utilise cumulatively – in growth and development – the temperature above a set basic temperature. Our phenology model examined the correlation between the sum of degree days and the date of sprouting (budburst). The basic temperature has been determined by optimization, above which (threshold temperature) the accumulation of daily means was most active, or alternatively, below which the daily means are most sensitively expressed in the phenology. The model has been extended to the calculation of the end of rest period (endodormancy) – by optimization as well. Our phenology model will be suitable for two main purposes: for estimating the time of budburst for the Hungarian region during the next decades calculated on the basis of regionally downscaled climate models; on the other hand, by applying our model, the risk of damage caused by spring frosts could be estimated more exactly than earlier.
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Overwintering capability and spring population size of honeybee colonies (Apis mellifera L.) in Hungary
153-156.Views:234Honeybee races and ecotypes of different genetic background have different population development in spring. Some of them can reach the necessary population size by the beginning of Robinia pseudoacacia (black locust) blooming period. There were significant differences in the spring population development between the colonies of different genetic background. The Italian races (A. m. ligustica) and their cross-breeds over-wintered poorly in Hungary, their spring population was low and they collected small amount of Robinia honey. The Austrian improved Carniolan (A. m. carnica) colonies over-wintered well, they had the largest spring population in both years. There was no significant difference between the size of the spring population of the same colonies of different genetic background in 1995 and 1996. The rate of the population development of the colonies was different in the two examined years. There was strong correlation (r = 0.8) between the spring population size and the Robinia honey yield, and between the mid-April population size and the Robinia honey yield of the colony groups of different genetic background. Spring population size also important in the effective pollination of fruit tree species that bloom earlier than the black locust trees.
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Organoleptic evaluation of sweet cherry varieties
7-13.Views:21092The extraordinary abundance of precipitation in 2010 (somewhere around 1000 mm) influenced highly the development of taste and flavour in cherry fruits. As stated, only a few varieties out of 27 were acceptable as for commercial quality. Under the given climatic conditions, actually ‘Bigarreau Burlat’ earned 965.11 points and proved to be the best in the early ripening group. In the mid season group, three varieties earned more than 1000 points (‘Giant Red’, ‘Carmen’ and ‘Vera’ grown at Nagykutas and Pallag), whereas in the late ripening group ‘Germesdorfi’ sent from Csenger (1084), ‘Linda’ (1070.07) and ‘Lapins’ (1052) received recognition. Correlation has been tested on the basis of 27 varieties between the individual properties. We sated that the most important attributes, which influenced the general impression of decision makers are the following: form and size of the fruit (r=0.835 and 0.797), furthermore juiciness (r=0.776), taste (r=0.876) and sweetness (0.875). Crispness was considered to be typical to cherry (r=0.743). Relation between acidity and sweetness was also essential to determine the acceptance of the cherry character.
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Stimulating effect of distilled water
47-49.Views:389It is an early observation that plants in poor soil are developing roots quicker and more abundantly than on rich one. There is a similar correlation between the nutrient status of medium and adventitious root formation.
In order to throw more light on the background of this strange phenomenon we started a systematic experimental program in which the biological effects of distilled water as model factor was investigated.
The experiments proved that the root formation of Pinto bean (Phaseolus vulgaris L.) cuttings with 3 cm long hypocotyls was promoted by distilled water.
The phenomenon above accompanied with slower decline and faster recovery of total and also water-soluble protein content, more intensive efflux of amino acids, greater amount of tryptophane and increased uptake of water compared to those in control hypocotyls. From other data obtained we may suspect that some additional active substance unknown for us also contributes to the stimulation of root initiation in distilled water.
