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Evaluation of the vegetative and generative performance of new apple cultivars in the Nyírség region
39-44.Views:258In this paper we examine apple varieties, which are planted expansively in the development apple orchards of the Western- European countries, but about its production characteristics we don’t have or just few practical experiences in Hungary. These varieties can be described with regular and high yields, aesthetic and alluring appearance in their origin places, what ensure profitable producing for the growers. Under the Hungarian climate which tends to be often extreme (hard cold winters, late spring frosts, summer drought, heat stress) these varieties may behave differently, so before the substantial planting it is expedient to carry out variety evaluations to avoid the later failures. The place of our experiments is located in the Nyírség representing adequately the ecological conditions of the surrounding areas, so the gained results can be adapted easily in the biggest apple production site of Hungary. Regarding the vegetative and the generative parameters of the 14 examined varieties (’Gala Venus Fengal’, ’Gala Decarli-Fendeca’, ’Galaval’, ’Jugala’, ’Gala Schnitzer (S) Schniga’, ’Red Cap Valtod (S)’, ’Early Red One’, ’Jeromine’, ’Crimson Crisp (Co-op 39)’, ’Red Topaz’, ’Wilton’s Red Jonaprince’, ’Red Idared’, ’Fuji September Wonder’, club cultivar) we found significant differences. The ratio of the thickness of trunk and the main branches showed that the trees have an optimal canopy structure in accordance with the Zahn principles, so they are in harmonious growing balance. The ‘Gala’ sport trained to slender spindle produced the highest yields, while the ‘Fuji’ and the ‘Red Delicious’ sport trained to super spindle can be described with the lowest values. All the cultivars reached the eating quality (65-70 mm), from which the ’Red Idared’, the ’Red Jonaprince’ and the ’Red Cap’ produced outstanding fruit size (81-85 mm).
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The effect of nectar production to the gathering behaviour of honeybees and to the foraging activity of wild bees at apple flowers
45-57.Views:183Intensity of bee visitation (honeybees and wild bees), foraging behaviour of honeybees and nectar parameters (nectar production, sugar concentration, sugar content in nectars) were inspected at 18 apple cultivars for three consecutive years. Honeybee visitation was calculated to be some 3.07 bee visits at a single apple flower a day but wild bee activity was very low, only some 0.11 wild bee visits per flower per day.
The nectar production (nectar content) of apple flowers was fairly different according to the cultivars and the nectar production (nectar content) of flowers was negatively correlated with the sugar concentration in nectar. Interestingly, at the majority of the inspected 18 cultivars the nectar production has shown minor or no changes in the morning and at the afternoon. The nectar production (nectar content) of flowers clearly encouraged the total bee activity at the inspected cultivars (r = 0.54). Bees visited abundant nectars with greater sugar concentration most intensely than lees abundant nectars with smaller sugar concentration.
Nectar parameters, however, affected the activity of honeybees with different types of gathering behaviour in different way. More intense activity by pollen gatherer and mixed behaviour honeybees was observed at cultivars producing abundant nectar (pollen gatherers: r = 0.65, mixed behaviour: r = 0.79). The activity of pollen gatherer honeybees and of mixed behaviour bees, on the other hand, was negatively correlated to the sugar concentration in nectar (pollen gatherers: r = -0.51, mixed behaviour: r = -0.73). This can be explained by the fact that their behaviour was much more affected by the amount of pollen than by any nectar parameters. Accordingly, "mixed behaviour honeybees" should probably rather be called as "pollen gatherers with nectar load" instead of "nectar gatherers with pollen load", as widely used in literature. The activity of ineffective side worker nectar gatherers was greatly encouraged by the sugar concentration in nectar (p = 0.63), similarly to the pure (top) nectar gatherer honeybees (r = 0.72). There was a negative relationship between the nectar production (nectar content) of flowers and the activity of these behaviour classes (nectar gatherers: r = -0.47, side workers: r = -0.36).
Concluding the findings we can state that the activity of pollen gatherers and mixed behaviour honeybees is strongly encouraged by greater nectar production (greater nectar content) of apple flowers. Their activity, however, is mostly dependent firstly on the amount of pollen. This is the reason why the sugar concentration is in a negative relationship to the activity of pure pollen gatherers and mixed behaviour bee. On the other hand, pure nectar gatherers and side worker nectar gatherers are greatly encouraged by the sugar concentration of apple nectar the amount of nectar was not a definite influence on their activity.
Side worker honeybees occurred at each cultivar inspected, however, their ratio varied widely among cultivars. So we can draw the conclusion that, in contradiction with earlier believes, side worker nectar gathering is a general phenomenon at apple flowers. The activity of side workers depends first of all on the relative position of stamens and petals; however, low sugar concentration of nectars can probably moderate their activity but probably do not affect pollen gatherers and mixed behaviour bees.
Wild bee visitation was very low; the wild bee species observed (Osmia cornuta, Andrena flavipes, Anthophora acervorum, Bombus terrestris, Bombus lapidarius and Halictus simplex) comprised some 3.44 per cent of the total bee visitation at apple flowers only. Wild bee visitation was in a positive correlation with the nectar production (nectar amount) of flowers (r = 0,4) and with the sugar content of nectar (r = 0.46) but it was negatively correlated with the sugar concentration in nectar (r = -0,27).
Our result calls the attention to the importance of nectar parameters in the effective honeybee pollination of apple plantations. Most effective pollinating activity of honeybees can be expected in plantations with cultivars of high nectar production because this feature seem so encourage the activity of those behaviour classes (pure pollen gatherers and mixed behaviour bees) that are the most effective pollinating agents among honeybees.
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Effects of salinity stress induced by hot spring water on tomato growth, yield and fruit quality under hydroponic cultivation in Japan
23-28.Views:166The objective of this research was to test hot spring water as a source of salt to improve tomato quality under the hydroponic system. This research was conducted at Yamagata University, in Japan from February to July 2017. Salt stress was induced using salts of hot spring wastewater collected from Yupoka Onsen (Tsuruoka, Japan). The treatments were EC 2, 4, 8 12, and 16 mS/cm which were arranged in a Randomized Complete Block Design (RCBD) with five replications. Tomato plants were grown at EC 2 until flowering and then subjected to different EC concentrations until harvesting. The data were collected on plant growth parameters and fruit quality. Fruits were harvested at the red stage until the 5th truss. The results showed that fruits' Soluble Solids Content, organic acid, Nitrate contents and Sugar: Acid ratio increased significantly at EC 16 mS/cm and in the upper trusses compared to EC 2 and in the lower trusses. In contrast, fruit weight significantly decreased at EC 16 mS/cm and in upper trusses. Leaf thickness, size and SPAD, and specific leaf weight significantly declined at EC 16 mS/cm and upper leaves compared to EC 2 and in lower leaves. Plant height started to decline significantly after three weeks of treatment at EC 16 compared to EC 2.
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Cost and profit conditions in the Hungarian intensive apple production
65-68.Views:220In this study I investigated the cost and profit conditions and the efficiency of intensive, qualitative apple production on the basis of a data collection carried out in ventures of high standard production. I concluded that the intensive apple production has an extremely high cost requirement, the production costs are approximately 1500 to 1600 thousand HUF per hectare. In an average case, a production value of 2000 thousand HUF per hectare may be reached, which may fluctuate in a wide interval during the years. Considering the above mentioned, a net profit of 400 to 500 thousand HUF may be realized in one hectare. It should be highlighted that regarding the present marketing conditions, realizing the appropriate profit may be expected only by producing 30 to 40 tons per hectare yields and 80 to 90% food quality ratio.
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Studies on the essential oil of different fennel (Foeniculum vulgare Mill.) populations during onthogeny
27-30.Views:228In the recent studies two Foeniculum vulgare Mill. populations (Hungarian and Korean) had been studied in open field trials in 1997-98 in Budapest for the identification of their essential oil characteristics. The essential oil accumulation level as well as the composition of the oil were studied during ontogenesis and at 6 phases of the umbel development from budding to overripening.
In the vegetative phases, the accumulation level of the essential oil was higher in the Hungarian genotype. In the leaf rosette stage, anethole is the main compound in both genotypes (40-96%), except the root of the Korean populations which contains 54% dillapiol. The accumulation level of anethole is slightly decreasing, while dillapiol is slightly increasing during the ontogeny. a- and [3-pinenes are characteristic compounds of the leaves, especially in the Korean genotype (10-11%). Before seed setting, fenchone was present in a considerable amount (7.5%) only in the umbels of the Hungarian genotype.
During the generative development, the maximum values of essential oil content are reached at the milky fruit stage (10,11%) in the Hungarian. and at the green fruit stage (7.1% ) in the Korean type, while the composition of the essential oil changed only slightly. The ratio of fenchone is increasing after flowering and being stable during ripening. Anethole varies to a smaller extent in the umbels, only.
We proved, that the dinamics of essential oil accumulation and the oil composition may vary according to genotype. Based on our results, the Korean population is characterized in the system of Bernáth et al. (2) as a high anethol-low methyl chavicol chemoform of the anethol chemovariety (fenchone<15%; anethole>68%; methyl chavicol< 3,2%).
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Effect of foliar spraying with algae suspension on leaf and fruit quality parameters of apple varieties
35-38.Views:265Foliar nutrition experiment was made to investigate the effect of algae products on fruit quality of apples (Malus domestica Borkh.). The study was conducted in 2011 at Siófok inWest-Hungary on cv. ‘Jonagored’ and ‘Idared’grafted on M9 rootstock. In our trial leaf diagnostic and fruit quality measurements were made to study the effectiveness of applied products. Relative leaf chlorophyll content was determined by a portable chlorophyll meter. Fruit weight and shape index were measured. Fruit inner qualifying parameters like titratable acid content and Brix value were also determined. It can be stated that the used products had traceable effect on investigated fruit parameters. Although, leaf N content was not effected by treatments significantly, relative leaf chlorophyll content significantly increased by both treatments. Used products significantly increased the fruit weight and shape index. Applied treatments significantly decreased the pH of pulp. Treatments increased the acid contents (fumaric, citric, malic) of apples and decreased the amount of monosaccharides. It means that the applied treatments pushed out the ratio of acid/sugar.
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The effect of different carbohydrates on the multiplication of Hosta cultivars
115-117.Views:159The effect of seven concentrations of two carbohydrate sources were compared to determine the best source and the most suitable source and concentration for micropropagation of some Hosta cultivars: H. 'Gold Haze', H. 'Gold Drop' and H. 'Dew Drop'. 0, 5, 10, 20, 30, 40 and 50 g/1 sucrose or glucose were added to a MS basic medium supplemented with 3 mg/1 kinetin and 0.1 mg/1 IAA. For 'Gold Haze' 40 g/1 sucrose proved to be the best source and concentration, the proliferation ratio was 15 shoots per explant. Thirty g/1 sucrose concentration was the optimum for 'Gold Drop', the proliferation rate was 14.6 shoots per explant. In 'Dew Drop,' the best results were obtained with 30 g/1 sucrose but 40 g/l sucrose gave good results too. Both cultivars rooted well on these media. On glucose containing media, very low propagation rates were found in all concentrations and all examined cultivars.
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Floral analysis can be use as an early plant analytical tool to diagnose nutritional status of fruit trees?
23-27.Views:224A field experiment was conducted in an integrated apple orchard (Malus domestica Borkh.) established on a lowland chernozem soil in East-Hungary, to investigate if flower analysis could be used to diagnose the nutritional status of the trees. In April 2008, during full bloom, flowers and leaves were collected. Leaves were collected again in August, at the standard sampling time from the same trees. The content of nitrogen, phosphorous, potassium, calcium magnesium and boron were measured in flowers and in leaves. Correlation analyses were carried out to establish the relation s between nutrient contents in same and different plant part s. In flowers the following ratio was found between nutrients: N:P: K:Ca:Mg:B -10: l.35:7: 1.7: I :0,02. From result s it was evident that flower as organ contain significant and comparable amount of nutrients like as leaf. The N, K and Ca content were higher in leaves than in flowers at full bloom. The opposite was true for B. The P and Mg content of flower were quite equal with leaf P and Mg at full bloom . All examined nutrients were significantly affected by cultivars both in flowers and leaves. Significant correlation, was found between flower P and leaf P, flower B and leaf B (P=O.O 1) and flower K and leaf K at blooming time (P=0.05). Significant, but weaker correlation was also found between flower K and leaf K and between flower Mg and leaf Mg al JOO days after full bloom (P=0.05). Moreover, strong, but negative correlation was observed between flower B and leaf B at 100 clays after full bloom (P=O.O 1). Within flowers, the strongest positive correlation was found between Mg and B content (P=O.O 1). Strong positive correlations were also recognised between flower K and flower N, Ca and Mg and between N and Ca in the flowers (P=0.01). The strongest correlation was found between K and P in leaves at full bloom (P=O.O 1 ). Strong significant correlation was observed between N and B in the leaves collected at standard sampling time (100 DAFB) (P=0.01). Strong, but negative correlations were found bet ween leaf Mg and leaf P, K and between leaf N and leaf P at 100 days after full bloom (P=O.O 1).
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Enzym Methods in Wine Analysis
67-70.Views:145In our laboratory for special determinations BOERINGER-MANNHEIM's enzyme-test combinations have been used for 10 years. Our present work deals with the practical aspects of the enzymatic determination of so important wine components like L-malic, L-lactic and citric acid, glycerol, D-gluconic acid, D-sorbitol, acetic aldehyde and D-glucose to D-fructose (G/F) ratio. Whenever possible, the results arc compared to the results of other methods (spectrophotometry, gas chromatography, polarimetry) used at our department.
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The uses of wolfberry (Lycium barbarum L.) as a fruit in an international breadth of view
7-13.Views:310Lycium barbarum is known exclusively as an ornamental plant in Hungary, and is planted so, as popular belief deem it a toxic plant. The plant’s fruit receives great respect in countries abroad, thus, due to its favourable content values the Lycium has achieved the title of ‘biological gold mine’. The last couple of years has seen the import of Lycium shrubs and its corresponding products, dried goods or in the form of various processed products which have been marketed and sold at extreme prices (under name of Goji, Wolfberry, Lifeberry). Our goal was to examine the similarities of the content values of wolfberry found in Hungary and those cultivated abroad. Along with Miklós Józsa the domestic Lycium population was surveyed based on foreign examples, between the years 2009 and 2011. Those defined sweet and largefruited were selected for further investigations to be set into a clone repository. This clone repository– which contains 67 different clones from a number of regions of Hungary – was established in the nursery of Dr. Miklós Józsa, located in the city of Szombathely. The phenological and morphological characteristics and the fruit ripening and quality indicators of plants in the clone repository were investigated. Six ‘best’ clones – selected based on flavour, disease-resistance and vegetative characteristics (plant size, fruit set, yield and fruit size) were analysed based on their content values. The control plant was a cultivar imported by a delivery service, found also in growing. In addition to the results of the selection, the results of the content values of the six selected ‘best’ clones (total soluble sugar content, glucose-fructose ratio, carotene content, FRAP value) is documented in this paper. Further, based on the resulting information the possibilities of the fruit’s utilization are suggested. Significant differences were measured in the vegetative characteristics of the clones (plant size, fruit set, yield and fruit size) and of those of
its inner content values. The resulting properties are considerable in regards of the plant’s introduction into cultivation and also utilization. Based on growing characteristics, more favourable clones were selected than the foreign varieties already in growing. Those clones selected by us have had similar or better content values than foreign breeds. The investigation of these content values is still in progress. -
Comparative evaluation of chamomile (Matricaria recutita L.) populations from different origin
91-95.Views:451In our research project under cultivation we examined 4 cultivars ('Soroksári 40', 'Lutea , 'Goral', 'Bona') and 28 wild populations of chamomile assuring all of them the same environmental conditions. Plant height, flower-diameter, essential-oil content, and the main terpenoid and flavonoid composition were analysed. The aim of our study was to establish the genetic background of breeding a new cultivar as well as encircle those natural habitats that give chamomile drug with the best quality.
In the case of plant height populations from the Great Hungarian Plain were lower than plants from Transdanubia and the control cultivars. Between the wild and the standard individuals we found significant differences with relevance to the flower-diameter. With regard to the essential oil content the populations were very heterogeneous even those, which came from the same habitat. Populations form Transdanubia and Nagyiván reached the essential oil level of the cultivars (0.721-0.931 gi100g), and 75% of the examined plants exceeded the minimum requirement of the PhHg VIII.
According to the essential oil composition our previous statement was confirmed that in the populations of Transdanubia and Northern part of Danube—Tisza Mid Region the main component is bisabolol-oxide A (30-41.2%), while plants native to the territory cast of the river Tisza are mainly characterised by a-bisabolol (32.3-48.4). In some samples the ratio of bisabolol-oxide B was more than 10%. The chamazulene content was higher in the cultivars selected to this component (above 20%), than in the wild populations (varied between 1.22 and 17.2%). Populations originated from the central part of Hortobágy region had extremely high apigenin content (10-13 mg/g), but in the case of chlorogenic acid, hyperoside and quercitrin we did not find any differences affected by the origin.
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Hail data analyses
99-103.Views:130Long term data of hail events of three meteorological stations (Budapest, Debrecen and Szeged) were investigated. The hail event frequencies show significant differences in the time period 1901–2000 when the first and the second half of the century are compared. The frequencies of hail events are higher in the first half of the century in case of Debrecen and Szeged while it is higher in the second 50 years for Budapest. None of the frequencies of hail events in between 2001–2008 are significantly higher, though the average of precipitation fell during these events is higher except for Szeged. We have found that the results about the monthly distribution of hail events differ from the ones in the literature; the ratio of hail events is significantly less in the vegetation period.
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Grapevine - and apple - replant disease in Hungary
29-33.Views:128Field experiment was conducted to study the replant problems of grapevine and apple. Plantings were in three different fields: on virgin soil, on apple replant soil and on vine replant soil. Each field was planted with 60 pieces of grafted vine (variety Bianca on rootstock Berl. X Rip. T.K. 5BB) and 60 pieces of grafted apple (variety Gloster on rootstock MM. 106). Fungicide (BUVICID K with 50% captan agent, 0.5 g/1 1 soil) and nematocide (VYDATE 10 G with 10% oxamil agent, 0.03 g/1 1 soil) treatments were used in the soil in order to identify the causal factor of the problem.
Biological soil test was conducted to test 17 soil samples of 11 wine districts and vine growing fields in plastic pots, under shading net. No root pieces were left in the soil. Two bud-cuttings of the Berl. X Rip. T 5C rootstock varieties were used as test plants. In each case, samples were taken from the vineyard and from the virgin soil. One fourth of the soil from the vineyard was left untreated and the other three part was treated with nematocide, fungicide or heat.
The results of the field experiment suggest that there was no problem growing grapevine after apple and apple after grapevine, but both species had been inhibited growing after itself. The fungicide and nematocide treatments did not succeed in determining the casual factor of the problem. Heat treatment of replant soil (in pot test) was useful in AS and VNS soils.
Results of biological soil test suggest, that grapevine replant problem do not occur in every vineyard. In fifty percent of soils, no significant differences between the treatments for shoot length, weight of cane, length, diameter and wood:ratio of the fourth internode were observed. In one case, difference was not found in any of the measured characters. However, fruiting bodies of Roesleria pallida (Pers.) Sacc. and the mycelium of Rosellinia necatrix Prill. were observed in this sample. In other samples, there was no significant difference between the treatments, but nematode and fungus infection appeared to be involved in increased shoot growth in nematocide and fungicide treated plants (mycelium of Rosellinia necatrix was detected). In other samples, the fungus infection caused significant difference between the virgin, untreated and fungicide treated soils and infection of Rosellinia necatrix was observed.
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Effect of seedling quality on growth, yield and quality of tomato (Solanum lycopersicum L.)
64-72.Views:311A two trial greenhouse experiment was carried out at Rwanda-Israel Horticulture Centre of Excellence located at Mulindi Station to evaluate seedling quality on growth, yield and quality of tomato. The seedlings were grown in different growing media and produced seedlings with varying quality indices. The growing media of peat moss 100% (T2) and sand + goat manure + carbonized rice husks 50%: 10%: 40% (T8) were revealed in seedlings with the highest mean quality indices of 31 and 28 respectively, while sand 100% (T2) presented the lowest quality indices during both trials. The transplants were planted in polybags filled with 2:1 of topsoil and kitchen manure arranged in a randomized complete block design (RCBD) with four replications. Collected data were subjected to analysis of variance and means were separated using HSD test at a 5% level of significance. The results revealed that the seedlings grown in T1 (S1) and T8 (S8) consistently presented tomatoes with better growth performance and yield. S1 and S8 produced mean yield of 93.59 and 92.35 t/ha respectively while S2 had the lowest yield with 53.86 t/ha. The fruit produced from seedlings grown in T4 (S4) had the highest mean sugar acid ratio of 5.88 but not significantly different from 5.61 and 5.44 of S1 and S8 respectively. Hence, there was a positive relationship among seedling quality and growth and yield performance of tomato but not in fruit quality.
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Influence of various mineral supply on sweet corn root development
49-52.Views:141Investigations into root strength have assumed particular importance with the emergence of American rootworm (Diabrotica virgifera-virgifera) as the number one pest, mainly as a result of monocultural production. Three treatments were applied in the trial: zero control treatment, treatment "A" (g/m2): 4, 47 N + 7, 89 P205 + 19, 74 K20 and treatment "B"(g/m2): 8, 94 N + 15, 78 P205 + 19, 74 K20. Measurements were made to find out whether the treatments had produced any significant difference between the root strength of the two varieties (normal sweet Puma F I and super sweet Dessert R75 Fl) at the two dates of measurement (tasseling, milk stage) in the year 2003. The treatments with a high phosphorous ratio resulted in an apparent increase in root strength with both varieties. There was a detectable difference in root strength also between the two points of measurement, suggesting that root development had remained stable. The treatments produced significant increase not only in the root strength but also in the yields of both varieties.
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The main colouring substance and essential oil components of different carrot varieties
46-49.Views:183Investigating colouring components it was found that the p carotene being the most important from the point of view of nutrition constitutes about 60-70% of the total colouring material, whereas the ratio of a carotene is 18-34%.
Among the varieties having a short growing season the common incidence rate of the a and 13 carotene is very high (92.49 %), even a lower total colouring material content (178.02 ppm) results in bright orange red carrot roots.
The incidence of a and carotene represents, however, only 84.24% of the similar total colouring material value (171.74 ppm) measured in Nantais.
Among the storage varieties Fertődi vörös has the highest value of total colouring substance (213.04 ppm) from which the common proportion of the two carotene (a and (3) compounds amounts to 93.77%.
When testing the essential oil components the cariophyllen shows the highest incidence rate, which has a negative influence on the flavour materials of the carrot. Among the early varieties in Nantais Forto this quantity amounts to 24.08 ppm which is almost twice as much as the amount measured in Nantais. We found a similarly unfavourable quantity in %lords &ids (25.47 ppm).
Looking at the beta-pinen and 1-limonen of a bactericidal effect the quantity of l-limonen is higher (1.36 ppm on the average of the varieties). Among the varieties that have a longer growing season and which are suitable for processing Fertődi vörös is the best in terms of essential oil and colour content. Its beta-pinen content was above the average of this variety (1.12 ppm) while its 1-limonen content was considerably high (1.26 ppm).
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Work quality assessment of a cherry sorting machine
57-62.Views:370The conditions of the cultivation of marketable cherries are diverse. Choosing the production site on the basis of the climatic conditions, selecting the most appropriate variety taking into account the region and the purpose of the production, utilization of the optimal production method, to ensure the required water and nutrients supply, frost and hail protection techniques, modern technology in crop protection, and professional performance of harvesting and handling, to name just a few of the most important issues. The objective of present study is to determine the qualitative characteristics of the UNITEC cherry sorting machine.
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Grapevine and apple replant disease in Hungary
57-61.Views:154Field experiment was conducted to study the replant problems of grapevine and apple. Plantings were in three different fields: on virgin soil, on apple replant soil and on vine replant soil. Each field was planted with 60 pieces of grafted vine (variety Bianca on rootstock Berl. X Rip. T.K. 5BB) and 60 pieces of grafted apple (variety Gloster on rootstock MM. 106). Fungicide (BUVICID K with 50 % captan agent, 0.5 g/1 1 soil) and nematocide (VYDATE 10 G with 10 % oxamil agent, 0.03 g/1 I soil) treatments were used in the soil in order to identify the causal factor of the problem.
Biological soil test was conducted to test 17 soil samples of II wine districts and vine growing fields in plastic pots, under shading net. No root pieces were left in the soil. Two bud-cuttings of the Bed. X Rip. T 5C rootstock varieties were used as test plants. In each case, samples were taken from the vineyard and from the virgin soil. One fourth of the soil from the vineyard was left untreated and the other three part was treated with nematocide, fungicide or heat.
The results of the field experiment suggest that there was no problem growing grapevine after apple and apple after grapevine, but both species had been inhibited growing after itself. The fungicide and nematocide treatments did not succeed in determining the casual factor of the problem. Heat treatment of replant soil (in pot test) was useful in AS and VNS soils.
Results of biological soil test suggest, that grapevine replant problem do not occur in every vineyard. In fifty percent of soils, no significant differences between the treatments for shoot length, weight of cane, length, diameter and wood:ratio of the fourth internode were observed. In one case, difference was not found in any of the measured characters. However, fruiting bodies of Roesleria pallida (Pers.)Sacc. and the mycelium of Rosellinia necatrix Prill. were observed in this sample. In other samples, there was no significant difference between the treatments, but nematode and fungus infection appeared to be involved in increased shoot growth in nematocide and fungicide treated plants (mycelium of Rosellinia necatrix was detected). In other samples, the fungus infection caused significant difference between the virgin, untreated and fungicide treated soils and infection of Rosellinia necatrix was observed.