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Leaf protein analyses in order to utilise the leaf shoot of artichoke
Published February 3, 2016
43-47

The constat growth of the Earth’s population brings with itself a higher demand for food and protein not only in human nutrition but also for the feeding of livestock. Currently, the feed industry is mainly built onseed-based protein, wherethebaseplant is soybean, which is large lycovered by imports in Hungary, similar toother European countr...ies. However, the long-term economically sustainable lifestock breeding demandschanges which has also worked out strategies. An alternative protein sources could be green leafy plants.

In current work the Jerusalem artichokes as an alternative source of protein was studied, compared to alfalfa as a valuable protein plant. Our results indicate that fiber fraction ofJerusalem artichoke shootswas 34 to 37% after pressing in the autumn period while alfalfa slightly lower values were obtained (30%). On the other hand extracted green leaf protein concentration was higher in alfalfa than in Jerusalem artichoke. Along with this higher protein content could be measured from the leaf protein concentration of alfalfa and almost each amino acids were more, as well comparing to Jerusalem artichoke.

Overall, the alfalfa proved to be advantageous as expected both in leaf protein extraction efficiency, both regarding the content of the protein in the Jerusalem artichoke. However, considering aminoacid composition and green biomass production, Jerusalem artichokecould be a promisingplant species asplant protein sourceinthefuture.

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Alternative protein sources in the nutrition of farm animals
Published September 5, 2018
21-31

Protein requirement and its demand of farm animals became one of the critical problems in nutrition on a global scale. Protein requirement has been an explicit demand for a long period with soybean meal and animal protein, but recently there are some limitations in relation to their use and the availability of the high quality fishmeal decrease...s constantly. For this reason there is increased demand for finding new protein sources which could be the alternatives of soybean meal and fishmeal. Alternative protein sources can be divided into seven categories, according to their origin. In different countries, their use depends on the availability in large quantity and at reasonable price.

There is a long tradition of using legume seeds, as alternatives of soybean. Most of them contain some anti-nutritive compounds, but it can be reduced with systematic selection. Oilseed meals are also generally use in poultry and pig nutrition, but those crude protein content varied, depending on the oil extraction technology. Green fodder and leaf protein was also proposed as alternative protein sources, but their use is limited, in particular because of the market price. The amount of bioethanol and starch industry by-products increases gradually in recent years, therefore those became alternatives of soybean meal, or in much less extend, fishmeal. However, amino acid composition of such by-products are far from optimal for poultry and pig; therefore, in the case of their use amino acid supplementation is necessary. Several novel protein sources are proposed in the last decade, such as algae or insect proteins. Recently, their availability and use is limited, but in the near future those would be alternative protein sources in monogastric animal nutrition.

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Connection of protein and amino acid content of forage and medium quality winter wheat varieties
Published November 2, 2009
101-107

We analysed the crude protein content, amino acid content, amino acid composition of four forage and milling III. quality winter wheat varieties (Magor, Hunor, Róna and Kondor) from their samples from five following years (2003, 2004, 2005, 2006, 2007). We found that quantity of essential and non-essential amino acids rose with increase in cru...de protein content. On examination of protein amino acid composition in relation to crude protein content we found that the crude protein content increased the quantities of the non-essential amino acids also rose, while those of the essential amino acids decreased as the lysine, the limiting amino acid of wheat. We also established that, as crude protein content increased, the biological value of the protein decreased.

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Connection of crude protein content and amino acid content of forage and medium quality winter wheat varieties in the relation of quantity and quality
Published December 21, 2008
73-79

We analysed the crude protein content, amino acid content, amino acid composition of four forage and milling III. quality winter wheat varieties (Magor, Hunor, Róna and Kondor) from their samples from five following years (2003, 2004, 2005, 2006, 2007). We found that quantity of essential and non-essential amino acids rose with increase in cru...de protein content. On examination of protein amino acid composition in relation to crude protein content we found that the crude protein content increased the quantities of the non-essential amino acids also rose, while those of the essential amino acids decreased as the lysine, the limiting amino acid of wheat. We also established that, as crude protein content increased, the biological value of the protein decreased.

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Insect base-protein: A new opportunity in animal nutrition
Published May 20, 2020
129-138

The consumption of insect protein, as well as its use in animal feeding, has become a trend. This trend is particularly receiving a lot of attention in animal nutrition because the current protein source of the highest quality in animal feeding which is fishmeal is unsustainable, expensive and its demand is higher than supply. Insect protei...n can be of great potential in combating world hunger as the world population continues to increase. The potential of insect protein is wide-ranging, it could improve the economics of feed production, lead to cleaner production due to its low environmental footprint.

This review focuses on the need, (nutritional and environmental) advantages of insect protein in feed production, as well as previous research findings.

The unanimous conclusion of the reviewed papers is that insect protein has a great potential in feed production in terms of nutritional value, environmental implication, sustainability as well as economic implication.

However, there is a need for the intensification of research on this alternative protein source in Europe, especially in aquaculture due to the approval of 7 insect species in aquafeeds by the European Union. Another reason for the intensification is that there is still much to be done as a result of past research which shows that growth effects vary based on species and inclusion level.

 

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Examination of French bean on organic and conventional farming of Research Centre of Nyíregyháza
Published September 2, 2009
87-98

This study presents the yield results of some French bean varieties in organic and conventional farming. This study presents the advantage of organic farming in environmental point of view and in nutrition. Sale of organic products is insured, there is solvent demand rather in abroad than in Hungary.
In Research Centre of Nyíregyháza had m...ade organic farming since 1994, at present on 74 hectares.
In the first trial, variety comparison with 9 yellow podded French beans in organic and conventional farming was conducted. Varieties: Carson, Cherokee, Debreceni sárga, Goldmine, Héliosz, Minidor, Sonesta, Sundance és Unidor. The following parameters were observed: the time of emergence and flowering, number of plants per plot, plant height and flowing green harvest. We weighed yield of the standardized, un-standardized and diseased pod fractions. The results were evaluated statistically with SPSS and Excell softwares.
Emergence had all at once, but the plants of organic farming were 5 days earlier at flowering and maturity, than conventional farming.
The plants in organic place were more developed than in conventional ones. Emergence was more uniform, the growth and the number of plant were square. Significant difference was not detected in plant height between two places. Most of the varieties examined had better total yield in organic place, than in conventional ones. Deviation depended on variety. ‘Sonesta’ and ‘Debreceni sárga’ had the best yield in both places. In
conventional farming choice can be expanded with ‘Unidor’ and ‘Sundance’. In organic farming choice can be expanded with ‘Minidor’ and ‘Carson’.
In both places the Sonesta, Debreceni sárga and Unidor varieties had the most standardized yield per hectare. In organic place Carson variety had good pod yield because it was infected less by diseases.
In the another trial we studied inside content values of some varieties on organic and conventional places. The parameters were observed: dry matter-, starch-, crude fibre-, crude protein content and amino acid content.
The rates of asparagin or glutamine acid were the highest, which was followed by serin or histidine. In asparagin content was the most deviation between conventional and organic farming.
Significant differences were between varieties in dry matter-, starch-, crude fibre-, and crude protein content both on organic and conventional places. Each variety had significant differences between organic and conventional farming.
Starch had strong and negative correlation with dry matter, crude fibre and crude protein content.
Budai piaci and Minidor varieties and BU-16 variety candidate had higher starch content and lower dry matter, crude fibre and crude protein content on organic place. Sonesta variety had almost equal dry matter content on both places, but crude protein content was higher with 10% and starch content was lower with 6% on organic place, than on conventional place. Paridor variety had almost equal starch content, but it had higher dry matter and crude protein content on organic place. 

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Effect of weather conditions on the protein content and baking value of winter wheat flour
Published May 23, 2006
83-94

We searched for connections between weather conditions (with its sub-parameters as precipitation and average temperature) and the yearly formation of two quality parameters (protein content and baking value) on three levels of mineral fertilization, based on the results of a variety comparison experiment on chernozem soil, to select those weath...er parameters and critical periods which have significant effects on the quality of winter wheat flour.
We established that the protein content of winter wheat flour can be increased with increasing levels of mineral fertilizers. Protein content is lower and has higher deviation during non-fertilized conditions in different cropping years than on higher fertilization levels. Thus, it seems proved again that quality (as protein content) is mainly formed by the crop year, but can be improved with adequate agricultural engineering (with mineral fertilization in the present case). The higher sum of precipitation in May, and the lower average temperature after flowering, have the highest increasing effect on the protein content of flour of the examined parameters. Based on the results of the examined period, the rainier and warmer term than average before flowering and lower – average amount of precipitation and colder circumstances are favourable for higher baking values. The analysis with data of decades, proves the importance of the first half of May and the middle of June as especially important periods for quality formation. An increasing nutrient supply has different effects on the varieties; mineral fertilization increased the baking value of GK Öthalom winter wheat variety in almost every case, but the second level of fertilization decreased it in half of the examined years. Additionally, mineral fertilization played a role in the stabilization of the quality of highlighted varieties.

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Effect of sowing time and planting space on change of yield and protein content of white lupine (Lupinus albus L.)
Published February 3, 2016
85-89

The hinge of the lupine crop cultivation technology is the sowing time and the determination of the breeding area. According to the literature the early sowing (as soon as possible to go to the soil) and larger growing area is recommended for seed production. Based on the results of our 2 years experiment, the effect of sowing time and differen...t spacing is important on yield of white lupine, and the effect of the sowing time is more significant. A decrease in the yield was observed after later sowings. The yield and the rate of its decreasing was varied in one year and among different years depending on wheather condition and sowing time.

The role of sowing time is significant for protein content of seeds. The average protein content decreased due to the later sowings in 2014, while 2015 the protein content of seeds in early sowing time and of plants sowed 4 weeks later was similar. We measured less protein content in the 2. sowing time sowed 2 weeks later than the first one. In the studied years the average protein content of seeds from different row spaces were not different.

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The changes of the most important quality parameters of szegedien triticale cultivars in long-term fertilization trials
Published February 3, 2016
21-26

We were monitoring the quality changes of 2 triticale cultivars from Szeged (GK Rege and GK Szemes) in Fülöpszállás, Hungary, in a longterm fertilizer trial in 2012/2013 and 2013/2014. The following fertilizer combinations were used: untreated control, single applied N and single applied PK, 30 and 60 kg ha-1 N or PK, and N and PK together ...in 30:30, 60:60 ha-1 ratio. We measured the following quality parameters: kernel hardness, crude protein content and farinograph quality number for wholemeal flour.

Based on the results, the N fertilization treatment was beneficial to the tested triticale culticars in terms of kernel hardness and protein content as both indicators increased. The efficiency of the treatment was proportional to the N dose rate. On the other hand, the applied PK treatment decreased the kernel hardness and crude protein values. On these two parameters, the PK free, and high N dosage treatment (N60P0K0) had the most positive effect. However, the single applied N dose had no significant effect on farinograph quality numbers of the wholemeal flours, but PK dose had significantly positive impact on the tested cultivars. The N30P30K30 treatment resulted in the highest farinograph quality number, thus the low PK and low N combination was the most efficient treatment. The correlation analysis of the tested quality parameters showed positive correlation (0.9965***) between kernel hardness values and crude protein contents. Nevertheless, we found strong negative correlation between kernel hardness values and the farinograph quality number of the wholemeal flours (-0.9720***), as well as in the case of crude protein contents and farinograph quality number of the wholemeal flours (-0.9796***).

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Production and disposition of purebred Jersey populations
Published November 24, 2008
89-96

In Hungary crossbreeding wit Jersey has been in practice since 2004. Uniquely in the Hungarian history of the Jersey breed two times fifty head pregnant heifers were imported in 2006. The aim of our study is to present through the description of the production and the type traits of the population the inherent possibilities of Jersey. The breed... is presented by the figures of the rolling lactations, 305 day milk production, correlations between the traits and by the results of the evaluation of type traits. Analyses of the data were done by using independent samples t-test.
Based on data of 87 cows the average number of days in milk  is 308 and 48 cows has finished lactation. During the rolling lactation they produced 5050 kg milk with 5.34% fat and 3.8% protein content.
The first lactation Jersey cows’ production when corrected to 305 days was 5089 kg milk with 5.35% fat and 3.81% protein. Between the herds there was significant difference in milk kg, amount of fat produced, protein content and amount of protein produced. On the two farms distribution of cows belonging to different production levels is very different. The biggest difference is when categorized by the amount of milk produced.
Correlation between the milk kg and fat kg is the greatest (r=0.950). Similar values were obtained for the correlation between fat and protein kg (r=0.919) and for the milk kg and fat kg (r=0,898). 
Disposition of the Jersey cows is very well balanced. Their stature, strength, body depth, dairy form, rump angle and pin width is favorable, the average scores are between 4.5 and 6.5. The leg of the cows is a bit sickly, and is hocking in a little with medium-low angled hoof. Average values of all traits describing the udder are between 4 and 5. Cows belonging to the two herds differ by disposition. Significant difference exists in the following traits: body depth, dairy form, pin width, rear leg - side view, rear leg, rear view, foot angle, fore udder attachment, teat length. The
Jersey population imported to Hungary has a final score of 78 points which equals with the “good” qualification. 

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A growth model to predict body weight and body composition of broilers
Published December 28, 2018
17-24

Models predicting the nutrient partitioning and animal performance have been developed for decades. Nowadays, growth models are used in practical animal nutrition, and they have particular importance in precision livestock farming. The aim of the present study was to introduce a broiler model and to provide examples on model application. The mo...del predicts protein and fat deposition as well as the body weight of an individual broiler chicken from digestible nutrient intake over time. Feed intake (FI) and the digestible nutrient content of the feed are inputs as well as some animal factors like: initial BW, feed intake at 1 and 2 kg of BW, precocity and mean protein deposition. The protein and energy metabolism is represented as in the classical nutrient partitioning models. The protein deposition (PD) is driven by digestible amino acid supply and is under “genetic control”, the so-called potential PD limits the actual PD if protein is oversupplied.

The authors discuss how the model can be used to simulate the animal response upon different scenarios. Examples are given to show that the diet might be limiting if some animal trait is changed. Applicability of the model has shown through running the model by using different feed strategies (three- vs five-phase-feeding) and variations with animal factors. In conclusion, growth models are useful tools to support decision making for defining the most suitable feeds used in a broiler farm. The model presented in this paper shows a high sensibility and flexibility to test different scenarios. By challenging the model with different inputs, the animal response in terms of changes in body weight and feed conversion can be understood more by studying the shift in deposition of chemical constituents. The examples provided in the present paper shows the benefit of using mathematical models and their applicability in precision nutrition. It can be concluded that the growth model helps to apply “from desired feed to desired food” concept.

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Nutritive value of Timothy (Phleum pratense L.) during primary growth in 2005
Published December 21, 2009
61-67

In this paper we analysed the change of the chemical composition and nutritive value of Timothy observed during the spring of 2005. The nutritive value of Timothy was observed between the end of April and the beginning of June relating to the following parameters: crude protein, crude fibre, crude fat, ash, N-free extract, net-energy growth, ne...t-energy lactation, net-energy maintenance, Metabolizable Protein Energy dependent, Metabolizable Protein N-dependent. We also analysed whether a relationship between the environmental factors that affect the
growing period of grasses and the chemical composition can be detected or not. While testing for correlation, the number of days from 1st January, the amount of heat accumulation, solar radiation and rainfall were considered as independent coefficients.
For the estimation of weather conditions we calculated the climate index. The observed year can be described as a year with a rainfall above the average and abundant solar radiation. A correlation can be detected between the change of parameters of nutritive value and the quality of the current year. In 2005 the result of the analysis of nutritive value was showed a significance difference with respect to each chemical composition at the rate of P<0.001 depending on the time when the samples were taken.
According to the outlined data it can be stated that the change of the value of crude protein and ash show decreasing tendency agreeing the research literature. In parallel with the change of nutritive value, the amount of crude fibre and N-free extract increased. However, the value of Net-Energy maintenance showed an alteration only in the first half of the observed period. The same alteration tendency can be detected in the Net-Energy growth and the Net-Energy lactation. 

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Application of the SDS-PAGE method for the characterisation of winter wheat flour quality
Published May 23, 2006
112-118

The principle, development and importance of the SDS-PAGE method are presented in this article. The SDS-PAGE method has become one of the basic methods of molecular biological research, because it is widely applicable and its sensitivity is excellent in the separation of wheat storage proteins.
We have shown the application of this method wi...th a concrete example. It was also tested whether, it was possible to obtain a better baking quality product from a large amount of poor quality less valuable wheat by fractioning the flour according to particle sizes after grinding. We studied the rheological properties of flours with different particle size fractions from the original flour. The baking quality of the original flour was B2. The 125-90 and 90-63 μm fractions have significantly better baking quality (B1) than the original flour. The protein contents of these flour fractions were also significantly higher than the protein content of the original flour. We had a question: what has influenced the baking quality: the protein content or other factors? We searched for an explanation on these results in the protein composition of the flour samples. We studied the distribution of glutenin-fractions by SDS-PAGE method and evaluated them. We found with correlation analysis that the amount of LMW-Glutenin D-group (52-60 kDa) is in a strong, negative correlation to the baking quality (r = – 0.855*). Therefore, the baking quality of flour samples was influenced by this glutenin fraction.

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Changes of chemical composition and nutritive values of Reed Canary Grass (Phalaroides arundinacea) in first growth
Published December 1, 2010
37-42

Change in chemical composition and nutritive value of Reed Canary Grass (Phalaroides arundinacea) were investigated in springtime in 2004-2006. The nutritive value of Reed Canary Grass was observed between the end of April and the middle of June relating to the following parameters: crude protein, crude fibre, crude fat, ash, N-free extract, ne...t-energy growth, net-energy maintenance, Metabolizable Protein Energy dependent,
Metabolizable Protein N-dependent.
It was also analysed whether a relationship between the climatic factors affecting the growing period as well the chemical composition of grasses and the annual weather were looked for. For the estimation of weather conditions the climate index was calculated. A correlation can be detected between the change of parameters of nutritive value and the quality of the current year. Between 2004 and 2006, the result of the analysis of nutritive value indicated a significant difference with respect to each chemical composition at the rate of P<0.01 depending on the time when the samples were taken. According to the outlined data it can be stated that the change of the value of crude protein and ash show decreasing  endency agreeing the research literature. In parallel with the change of nutritive value, the amount of crude fibre and N-free extract increased. However, the value of Net-Energy maintenance revealed an alteration only in the first half of the observed period. The same alteration tendency can be detected
in the Net-Energy growth. 

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Evaluation of the blood product characteristics of meat meal and hemoglobin with non-invasive methods in the VISNIR wavelength
Published March 23, 2016
49-56

 

The separate collection of poultry slaughterhouse trimmings and blood is partially solved in Hungary. Only properly prepared animal by-products, protein meals can be utilized as animal feed additive. Howev...er, different protein meals are appropriate for feeding different animal species. That is the reason why it is important to avoid accidental cross contamination of the products. Meat and blood meal produced on the same technological line, therefore mixing of the products can happen in various proportions during the shift of production.

 

Thus the aim of this study is to develop a spectral method which will allow to estimate the ratio of meat and blood protein meal in the final product. During the test the products were mixed in different proportions and were examined by the spectral method. Measurements were conducted with AvaSpec 2048 spectrometer in visible (VIS) and in near infrared (NIR) wavelength range (400–1000 nm) to define the spectral differentiation of the different meal products. Significant difference can be detected in spectral reflectance between the meat and blood product in the VIS-NIR range. The blood product has a characteristic spectral property: in the range of 600 and 735 nm reflectance values are increasing following a sigmoid curve. This property is not observed in the case of meat meal: close to linear rising is detected. Effective protein rate and purity detection could be made by Blood Product Sensitive Mixing Index (BPSMI – R930/R600), and by the calculation of inflection point in 600–735 nm.

 

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The effect of different fertilizer treatments on the sulphur and protein content of wheat
Published July 31, 2012
73-76

In this study the effect of N,P and K nutrients on the S and protein content of wheat grains was investigated in a long-term fertilization experiment set up in Nagyhörcsök. The calcareous chernozem soil having the following characteristics: pH (KCl): 7.3, CaCO3: 4.27%, humus: 3.45%, Al-soluble P2O5 and K2...O: 60–80 and 180–200, KCl- Mg: 150–180, KCl+EDTA-soluble Mn-, Cu- and Zn-content: 80–150, 2–3 and 1–2 mg kg-1. The experiment had a split-split-plot design with 40 treatments in 4 replications. Plant samples were collected from 2002 and 2004. 2002 was a drought year while 2004 was very wet.
The main conclusions are as follows:
– The sulphur and protein content were than the control higher in every NPK treatments.
– The sulphur and protein content of the wheat grains were higher in 2004 that had a lot of rain than in 2002 that had drouht.

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Agronomic bases of precision barley production
Published November 13, 2012
217-220

To ascertain the importance of individual preciosion factors in achieving yield and quality of malting barley, we established a multifactor 
experiment at the research station of the Slovak University of Agriculture in Nitra in 2009 and 2010. Four variants of fertilization, 2 ways of soil cultivation and four varieties 'Bojos’, 'Kango...o’, 'Marthe’ and 'Xanadu’ were observed. From the quality indicators the nitrogen content (%) was observed. The results were statistically analyzed by using a multifactor analysis of variance by using Statistica 8, the program Statgraphics. The difference between years was statistically significant (1.87 t ha-1) in favour of 2010. The difference was also significant between the varieties 'Bojos’ and ’Kangoo’(1.07 t ha-1), respectively. 'Martha’ and 'Xanadu’ as well as 'Kangoo’ (0.56 resp. 0.33 t ha-1). Conventional soil cultivation in
comparison with a minimalization technology, demonstrated a  tatistically insignificant increase of yield. Significant differences were obtained between the variants of fertilization. Treatment by using Condit (b-var.) showed a very positive impact in climatic favorable year (2010) with a yield result of 7.42 t ha-1. In comparison with an untreated control, the difference in yield was 0.89 t ha-1. A significant increase of yield was achieved by using the combination of solid fertilizer with foliar fertilizer (LAV + Hakofyt, var.c) 0.47 t ha-1. The crude protein content was statistically influenced by a variety and year. In 2009, the crude protein content was above average (12.38%). Significantly lower attributes were achieved in 2010 (9.90%). From the studied/observed varieties the lowest crude protein content was showed by a variety 'Kangoo’ (1.68%).

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Comparative study of different soybean genotypes in irrigation technology
Published May 23, 2019
91-95

In many places in Hungary, early maturity soybean can be successfully grown. The earlier maturity group of soy which ripened in 110–125 days in most crop areas in Hungary. However, to achieve excellent results, the selection of proper varieties is important too. Successful cultivation is largely dependent on the macro and microclimate of the ...production area, the nutrient supply of the soil and the cultivation technology. Soybean can be produced in places where the amount of precipitation is right, as the lack of water results in lower yields and deteriorated oil and protein concentrations. In the following study, 2 years (2016 and 2017) are compared to the yield, protein and oil content of the soybeans of the early maturation group in irrigated and non-irrigated treatments. Based on our experiment, it can be stated that, during the irrigation of soybean, oil and protein content and yields did not always change.

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Comparative analysis on the fertiliser responses of Martonvásár maize hybrids in long-term experiments
Published December 21, 2008
111-117

The results of experiments carried out in the Agricultural Research Institute of the Hungarian Academy of Sciences clearly show that in the case of hybrids grown in a monoculture greater fertiliser responses can be achieved with increasing rates of N fertiliser than in crop rotations. In the monoculture experiment the parameters investigated re...ached their maximum values at a rate of 240 kg/ha N fertiliser, with the exception of 1000-kernel mass and starch content. In both cases the starch content was highest in the untreated control, gradually declining as the N rates increased. Among the parameters recorded in the crop rotation, the values of the dry grain yield, the 1000-kernel mass, the protein yield and the starch yield were greatest at the 160 kg/ha N fertiliser rate, exhibiting a decrease at 240 kg/ha. Maximum values for the protein content and SPAD index were recorded at the highest N rate. It is important to note, however, that although the N treatments caused significant differences compared to the untreated control, the differences between the N treatments were not significant.
In the given experimental year the values achieved for the untreated control in the crop rotation could only be achieved in the monoculture experiment at a fertiliser rate of 160 kg/ha N, indicating that N fertiliser rates could be reduced using a satisfactory crop sequence, which could be beneficial from the point of view of environmental pollution, crop protection and cost reduction.
The weather in 2006 was favourable for maize production, allowing comparative analysis to be made of the genetically determined traits of the hybrids. Among the three hybrids grown in the monoculture experiment, Maraton produced the best yield, giving maximum values of the parameters tested at a fertiliser rate of 240 kg/ha N. The poorest results were recorded for Mv 277, which could be attributed to the fact that the hybrid belongs to the FAO 200 maturity group, while the other hybrids had higher FAO numbers. Maraton also gave the highest yields in the crop rotation experiment at the 160 kg/ha N level. All three hybrids were found to make excellent use of the natural nutrient content of the soil.
It was proved that the protein content of maize hybrids can only be slightly improved by N fertilisation, as this trait is genetically coded, while the starch content depends to the greatest extent on the ecological factors experienced during the growing season.

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Effect of the fertilization on some quality parameters of winter wheat
Published December 15, 2010
105-110

We studied the different mineral fertilizer rates effect on the baking quality of five different winter wheat genotypes (GK Öthalom, Lupus, KG Széphalom, GK Békés, Mv Mazurka) on chernozom soil in 2008.
The bigger fertilizer doses increased the wet gluten content, gluten elasticity and the protein content. The high doses of fertilization... has got a moderate effect on the valorigaph value, water absorption, and falling number. The optimum level of the fertilizer doses were the N90-120+PK.
The Pearson’s correlation analysis proved strong relationships between the fertilization and gluten content, the quality of the gluten, a valorigraph value, and the protein content. The analysis verified a strong correlation between genotype and falling number, and the protein content.
The variety Mv Mazurka has got the best baking quality parameters.

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Lack of polymorphism of the agouti signaling protein (ASIP) gene among four different brown hare (Lepus europaeus Pallas 1778) populations
Published October 24, 2016
81-85

The brown hare (Lepus europaeus Pallas 1778) is a common palearctic and a popular game species therefore it has been an obvious subject for population genetic studies since the second part of the 20th century. Among the several mitochondrial DNA studies some have been carried out concerning nuclear genes as well. The agouti signaling... protein gene (ASIP) is involved in regulating the synthesis of eumelanin and pheomelanin in melanocytes of mammals. Though many studies focused on it in relation with several mammalian species, minimal information is available on this topic concerning the brown hare.

Here we present a short communication concerning the agouti signaling protein (ASIP) gene in four different country’s L. europaeus populations, namely Lithuania, Hungary, Serbia and Georgia. N=45 tissue samples have been investigated from overall 17 sampling sites of the different countries. There has not been found any polymorphism among the sequences. In an alignment with other Leporid species’ partial ASIP sequences downloaded from ENA we have found that based on a 178 base pairs long DNA sequence the haplotype of our samples contains three other Lepus species as well. This is concordant with the findings of a previous study focusing predominantly on the European rabbit (Orycto lagus cuniculus Linnaeus 1758) and the several mutations of its ASIP gene.

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The effect of different genotype cattle grazing on the nutrient content of saline grasslands vegetation
Published December 28, 2018
46-50

Maintaining saline grasslands in good condition, preserving their yield and diversity is important not only for the purpose of nature conservation, but also for farming. Therefore, the primary purpose of our study is to analyse the effect of pasture use of the smaller weight extensive and the larger weight intensive beef cattle on the grassland... vegetation and nutrient content. In this way, we can answer the question whether grazing for nature conservation can be achieved with more profitable, more economical and more intense varieties.

The tests were carried out in May 2016 and May 2017, in the Hortobágy National Park (Pap-ere and Zám puszta), where a total of 16 sample areas were analysed. These areas are grazed with extensive beef cattle (Hungarian grey) and mixed genotype of intensive cattle. The associations were selected along a moisture gradient, such as wet salt marsh (Bolboschoenetum maritimi) and drier saline meadow (Beckmannion eruciformis). All the vegetative material collected both years in May was analysed for the following parameters: dry matter, crude protein, crude fibre and life-sustaining net energy content. We compared the effects of medium grazing (0.46 livestock/ha) and abandonment on vegetation and nutrient content.

We examined the effect of (i) grazing, (ii) different grasslands (salt marsh, saline meadow) and (iii) grazing of different cattle breeds (Hungarian grey, intensive beef) on the nutrient content of the vegetation of grasslands Based on our results, it was found that grazing had an impact on crude protein and life-sustaining net energy content. The highest crude protein content (12.75 m/m%) was obtained in the year 2017 in the area where higher density had been grazed for two years. For the lifesustaining net energy, the highest value (5.05 MJ/kg d.m.) was also obtained in 2017 and the lowest in 2016. Furthermore, it was found that there was no significant difference between the effect of the two cattle breeds on the parameters examined. Significant effects were observed only in the case of life-sustaining net energy: in the area of intensive beef cattle we received a higher value (5.15 MJ/kg body weight) than in the area with extensive beef cattle (4.96 MJ/kg body weight).

Our results have also shown that cattle grazing is of the utmost importance for the maintenance of both wet and mesophilous habitats. Based on our three-year study, we can say that grazing by both extensive and intensive cattle breeds is suitable for the management of saline habitats.

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Fractionation of chicken egg proteome by isoelectric point in liquid phase
Published March 20, 2014
39-42

The application of proteomics is relevant to physiology, reproduction, immunology, muscle and lactational biology in animal science, altough its use is still limited. One of the greatest challenges of proteome analysis is the reproducible fractionation of the complex protein mixtures. The fractionation methods can increase the probability of bi...omarker protein discovery. The fractionation by liquid-phase isoelectric focusing is one of the prefractionation methods. As a result, protein fractions can be easily collected, pooled and refractionated. There is a lack in the knowledge of gel-based proteomic methods of egg as only a limited number of protocols can be found in the literature, thus sample purification and fractionation require a time consuming optimisation procedure. The aim of this study was to fractionate egg yolk and white proteins by isoelectric point in liquid phase.

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Stability analysis of different winter wheat genotypes in long-term experiment
Published December 16, 2012
135-141

Nowadays, due to the climate change, it is becoming increasingly important in the occasionally extreme years that the yield and the quality
parameters of a given winter wheat variety should not fluctuate at all or only slightly under similar agrotechnical conditions as a result of the year effect. In four years (2005–2008) we studied the c...hanges in the wet gluten content, gluten speading and protein content of five wheat genotypes at six fertilization levels.
In the control, it can be observed that the year had a significant effect on the wet gluten content, the protein content of the flour and gluten speading, therefore, a great fluctuation was detected in these qualities of the varieties in the four studied years. At the optimum fertilization levels (N120-150+PK), the varieties Sixtus, Saturus and Lupus showed a much lower fluctuation and more stable values were measured. The most stable variety in the control treatment was Mv Mazurka for all the three quality parameters, while at the optimum fertilization level (N120+PK), the most stable results were obtained for the variety Sixtus as an average of the four years.
When studying the results using Kang’s method for stability analysis, it can be stated that the most stable values of wet gluten content were obtained at the fertilization level of N120+PK under a variable year effect, the varieties gave also the best gluten content values at this level. The most stable protein content values of flour were obtained at the fertilization level of N60+PK. The results showed that the fluctuation of quality parameters as a result of the changing years differed between the different winter wheat varieties due to their differing genotypes, but this fluctuation could be reduced or minimized by a proper fertilization. 

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The hydric stress influence on quantityand quality of the maize yield in the Crisurilor Plainconditions
Published May 6, 2013
21-27

...5); font-variant-ligatures: normal; font-variant-caps: normal; -webkit-text-stroke-width: 0px; text-decoration-style: initial; text-decoration-color: initial;">The paper is based on the researches carried out in an experiment placed on the preluvosoil from Agricultural Research and Development Station Oradea, Crisurilor Plain during 2008–2011in the following variants: V1=Irrigated, without irrigation suspending; V2=Irrigated, irrigation suspending in May; V3=Irrigated, irrigation suspending in June; V4=Irrigated, irrigation suspending in July; V5=Irrigated, irrigation suspending in August; V6=Unirrigated. The hybrid used: Fundulea 376. In the variant with optimum irrigation, water reserve on 0–75 cm depth was maintained between easily available water content and field capacity. Pedological drought was determined every year and the irrigation was also needed. The irrigation determined the increase of the total water consumption and yield gain in comparison with unirrigated variant. Irrigation suspending in different months determined the yield losses very significant statistically. The biggest protein content was registered in the variant without irrigation suspending; the values registered in the variants with irrigation suspending in May, June, July and August and in the unirrigated variant are smaller, with differences statistically assured. There was a direct link between de Martonne aridity index values and water consumption, yield and protein content and an inverse link between pedological drought and yield quantity and protein content. These are the arguments for irrigation opportunity in maize from Criurilor Plain.

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