Search
Search Results
-
Micropropagation of Rudbeckia hirta L. from seedling explants
53-59Views:194We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding.
-
Utilization of Okra (Abelmoschus esculentus L. Moench.)
122-125Views:200Okra is warm season crop native to Africa. Presently it is widely grown in the northeastern parts of Brazilia, both northen, and western parts of Africa, southeast Asia, and in the eastern parts of the Mediterraneum. In Hungary it is a rarity. It’s adaptation to Hungary began about 40 years ago.The optimal sowing time under Hungarian climatic conditions is middle of May. The harvests is started – depending on the weather – first, by the middle of July, and picking continues until the first of September.
According to our results, seed soaking, sowing under a perforated foil sheet-covered trench (2 weeks earlier the normal sowing time), and 55-65 g thousand weight seeds are favourable.
The harvest period under Hungarian circumstances is appr. one and half months. -
Alternative protein sources in the nutrition of farm animals
21-31Views:608Protein requirement and its demand of farm animals became one of the critical problems in nutrition on a global scale. Protein requirement has been an explicit demand for a long period with soybean meal and animal protein, but recently there are some limitations in relation to their use and the availability of the high quality fishmeal decreases constantly. For this reason there is increased demand for finding new protein sources which could be the alternatives of soybean meal and fishmeal. Alternative protein sources can be divided into seven categories, according to their origin. In different countries, their use depends on the availability in large quantity and at reasonable price.
There is a long tradition of using legume seeds, as alternatives of soybean. Most of them contain some anti-nutritive compounds, but it can be reduced with systematic selection. Oilseed meals are also generally use in poultry and pig nutrition, but those crude protein content varied, depending on the oil extraction technology. Green fodder and leaf protein was also proposed as alternative protein sources, but their use is limited, in particular because of the market price. The amount of bioethanol and starch industry by-products increases gradually in recent years, therefore those became alternatives of soybean meal, or in much less extend, fishmeal. However, amino acid composition of such by-products are far from optimal for poultry and pig; therefore, in the case of their use amino acid supplementation is necessary. Several novel protein sources are proposed in the last decade, such as algae or insect proteins. Recently, their availability and use is limited, but in the near future those would be alternative protein sources in monogastric animal nutrition.
-
Statistical comparison of soil analysing results of chernozem soils
93-99Views:311The soil fertility was degraded as a result of unreasonable tillage, therefore the application of site-specific nutrient replacement is necessary. It is essential for the application of precision fertilization to know the location, extension, soil properties and nutrient-supply of the different soil types of
cultivated areas.
We collected soil samples from 580 hectares of land in 2006. Soil samples were collected from every 5 ha in 30 and 60 cm depths during Spring from 20.05.2006 to 12.06.2006 and again in Autumn from 09.19.2006 to 02.10.2006. Soil samples were analysed at the Department of Agricultural Chemistry and Soil Science of DE-ATC.
The two examined soils are slightly calcareous, weakly saline, poor in zinc. The calcareous chernozem soil is slightly acid, the content of humus, nitrogen, phosphorus and potassium is medium in this soil. The meadow chernozem soil is slightly alkaline, and properly supplied with humus and potassium, and middling supplied with nitrogen and phosphorus. The meadow chernozem soil is more heterogenous in soil plasticity, lime, saline, nitrogen phosphorus and potassium content and less heterogenous in pH and zinc content than the calcareous chernozem soil.
Standard deviation of measured values in pH, soil plasticity, humus and nitrogen content significantly differ between the examined soil types. The soil plasticity, pH, humus, nitrogen and zinc content significantly differ among calcareous chernozem soil and meadow chernozem soil, but the difference in phosphorus content can be statistically proven only in case of Spring soil sampling. -
Presence, feeding and health status of collared doves (Streptopelia decaocto Friv. 1838) on animal breeding farms
49-54Views:253In the course of our examinations we were determining the gender of collared doves (Streptopelia decaocto) feeding at animal breeding farms by biometric data. We were estimated their nourishment and necropsies were carried out.
We compared the results of the nutrition analysis with similar data of collard doves feeding at agricultural areas.
We analyzed the crop and gizzard content and recorded the body proportions of collard doves that were captured between December of 2004 and August 2005. In the course of the necropsy examinations we did parasitological, bacteriological examinations as well as examinations for the detection of Newcastle disease. In the course of the gender analysis of the collard doves that were collected at animal breeding farms, both the wing length and the length of the third quill-feather has shown a significantly greater value in the case of males (p>0.05).
From the orts found in the crop and gizzard it has been stated that the feed consumed the most was corn (80.77% m%). The collared doves consumed much less other cereal seeds (wheat 0.82%, barley 1.26%).
In the course of the necropsy examinations done in the Veterinary Institute of Debrecen, the birds proved to be free of parasites, and the results of the Salmonella and Newcastle disease analysis were negative as well. E. coli bacteria had been detected in the liver of not more than three birds. -
Evaluation of chickpea (Cicer arietinum L.) in response to salinity stress
105-110Views:682Soil salinity is a severe and expanding soil degradation problem that affects 80 million ha of arable lands globally. Chickpea (Cicer arietinum L.) is very sensitive to saline conditions; the most susceptible genotypes may die in just 25 mM NaCl in hydroponics. Approximately 8–10% yield loss in chickpea production is estimated due to salinity stress. However, it is still not established why chickpea is so susceptible to salt affection. Salinity (NaCl) impedes germination of seeds, though chickpea varieties considerably differ from one another in this respect. Some chickpea genotypes are more tolerant in the stage of germination, tolerating even 320 mM NaCl. The reasons of this variation are unrevealed; there is a shortage of knowledge about the germination abilities of chickpea genotypes in saline conditions. Nevertheless, the effect of salt stress on vegetative growth can be analysed in hydroponics, in pot or field conditions, regardless the experimental environment, the ranking of genotypes regarding salt resistance is coherent. Chickpea genotypes can be different in their ability to retain water, maybe under salt affection; the more salt tolerant lines can maintain higher water content in the shoots, while the more sensitive ones cannot. The identification of salt tolerant chickpea landraces based on developing genetic variability is a suitable strategy to combat against salinity problems arising in arid and semi-arid areas.
-
Fruit melanotic ringspot (FMRS) – a disease of resistant Capsicum genotypes infected with Tomato spotted wilt virus (TSWV) on the fruits
64-69Views:145Etiology of pepper fruit melanotic ringspot (FMRS) disease (Salamon, 2009) was studied on fruit samples collected in forced pepper populations. It was noticed that in spite of heavy thrips (Frankliniella occidentalis) infestations and of TSWV epidemy detected in the forcing houses, FMRS occurred only in plants having healthy foliage. Symptomatological surveys strongly suggested that FMRS appeared exclusively in specific pepper genotypes. The size of melanotic ringspots has been observed to grow at room temperature during postripening of diseased fruits. A mechanically transmitted plant virus was isolated from symptomatic parts of 9 white pepper fruits affected by FMRS. On test plants each of the virus isolates caused systemic symptoms characteristic to TSWV. Using cDNA/PCR technique and TSWV N-gene specific primers a ca. 300 bp long DNA fragment has been amplified from total nucleic acid extracted from symptomatic tissues but never from asymptomatic parts of the fruits showing FMRS. Plant progenies grown from seeds of FMRS diseased fruits segregated in respect of resistance and/or susceptibility to TSWV infection. TSWV was also detected in and isolated from three fruits showed non-melanotic yellow rings (one of them was infected with a tobamovirus, too). Seedlings derived from these fruits proved to be susceptible to TSWV. Based on the above results we could conclude that the FMRS disease developed on fruits of “cecei” type white peppers that carry a TSWV resistance gene, most likely the Tsw gene in heterozygous form. These fruits were infected with thrips transmitted TSWV and FRMS appeared as a hypersentive reaction (HR) manifested in fruits.
-
Application of AFLP-Method in Plant Sample Identification
207-213Views:237One possible method for the determination of DNA-polymorphism is the PCR-based AFLP (Amplified Fragment Length Polymorphism). This method had been succesfully introduced to the Department of Botany at University of Debrecen in 2000-2001 with the examination of hay saffron (Crocus sativus L.) and its allies. Hay saffron is grown as a spice for some thousand years producing the most expensive spice in the world. This plant is sterile, triploid reproduces only vegetatively with no fertile seeds. However its origin is unknown it exists only in cultivation and it is a mutated variety of another species or an artificial or natural hybrid. Usual methods for the systematic examination are restricted hence it seemed to be reasonable to apply molecular biological methods in its case. Results of this work include the introduction and many fold application of the method beside ensuring the consequences of science literature with determining the C. cartwrightianus to give the most similar genetical pattern to C. sativus.
-
Evaluation of decreasing moisture content of different maize genotypes
147-151Views:545An experiment was conducted to evaluate the decrease in grain moisture content in three maize hybrids in Debrecen in 2017. Armagnac, Sushi and Loupiac were the examined hybrids in this study. The culture medium and temperature conditions were applied uniformly for all three hybrids. According to the results obtained from the ratio of moisture content of seeds per day, the Armagnac variety in the intensive drying down phase loses more time and moisture content, so it can be concluded that the produced dry matter is more than in the case of other varieties. Armagnac requires more time to achieve yield, while the Sushi and Loupiac hybrids produce less harvest. Regarding the slope of the regression line, the rate of loss of moisture in the grain has been negatively correlated with the amount of “b” in the three examined hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97% in the Loupiac, 95% and in the Sushi 90% of the total dynamic value of moisture motion.
-
Habitat suitability modeling of a Subterranean mammal in Iran: case study of the Western Mole-Vole (Ellobius lutescens)
113-120Views:444Rodentia is the largest order of mammals in Iran, distributed in almost all types of habitats. Subterranean mammals, such as the Western Mole-Vole, have an important role as ecosystem engineers by creating underground burrows and tunnels and aerating, propagating seeds, and building shelter for other small mammals and animals. They are also a keystone part of the food source for other prey vertebrates. However, many areas are considered pests, and their habitats are fragmented due to human activities, which causes a reduction in their habitats and colonization. Habitat Modeling tools help us to predict a suitable niche for conservation planning. The purpose of this study is to predict habitat suitability for Western Mole-Vole in Iran by using the MaxEnt method to determine which part of Iran is a desirable habitat for this species. The result indicated that the northwest of Iran, especially the southern slopes of the Alborz Mountains and the Eastern slopes of the Zagros Mountains, provides suitable habitats for the western Mole-Vole. The environmental variables that are more influenced by the distribution of this species are elevation of 1800 to 2500 meters, human activities such as roads, and rainfed farms. Approximately 7% of Iran is a suitable habitat for the Western Mole-Vole.
-
Product orientated sewage sludge composting technologies in the practice of AKSD Ltd.
33-36Views:338In recent years the regulations of the EU unambiguously determine that the biodegradable wastes should be used for biogas production or composting. The direct use of sewage sludge in agriculture is limited by its hazardous microelement content, but it can be used effectively in composting. The limitation parameters can be extinguished with correct compost-mixture and technology variants. The exact determination of the technologies and mixtures result a stable, constant quality product. The compost, as a classified product, is friable structured. The end-product is a
homogeneous mould with good water capacity, does not contain any weed seeds and pathogenic organisms. The compost does not have a acidify effect on the soil. It is an effective fertilizer thanks to its macro and microelement content. -
The effect of gamma irradiation on the germination and growth of Turda Star maize hybrid dry samples
23-25Views:136In order to study the effects of gamma rays on germination indices i.e. germination index (GI), total germination (GT) and the
germination energy (GE), of maize hybrid, Turda Star current experiment was conducted. Dry seeds of Turda Star maize hybrid were
irradiated with 2, 5, 15, 30 and 50 Gy, using a 60Co-gamma radiation source.
Results showed that gamma irradiation affected all the above mentioned parameters except germination percentage. Total
germination and germination energy was significantly affected at higher doses of gamma rays. However, increasing doses of gamma
rays did not have significant effects on seed germination percentage. The other growth parameters showed declining tendency with
increasing doses of gamma irradiation. -
Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)
93-96Views:247Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered next to Debrecen city (Hajdú-Bihar county, East Hungary).
In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.
The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.
-
Gene Bank Developed by Induced Mutation for Selection
45-49Views:198Heterosis breeding in maize caused gene erosion by using uniform inbred lines. In order to strengthen the genetic base, we established a gene bank containing lines with broad genetic variability, resistance and adaptability. The maize gene bank is a result of our work in the past two decades.
The gene bank originated from treatments of maize seeds of hybrids and inbred lines with fast neutrons. The 1500 maize lines have great genetic variability which can be exploited after strict assessment and selection. As a result of the past several years, P 26, P 61 and P 62 lines have been released after DUS investigation in 2001. -
Stand evaluation, crop estimation and yield analysis of winter wheat for the optimization of yields
103-109Views:207The authors have been carrying out stand evaluation, crop estimation and yield analysis in winter wheat since 2012. The sampling areas were assigned at the fields of the Training Farm of the Faculty of Agricultural and Food Sciences of Széchenyi István University Mosonmagyaróvár according to the structure of the cropping system. According to their observations the value of field emergence is always lower than the laboratory germination. The weak emergence is important because the lower plant density cannot be compensated by the increased tillering in spite of having larger plant growth space. It is proven by the fact that they detected strong productive tillering even at 5 and 10 mm plant spacing while there were single-spiked plants at 40-50 mm plant spacing as well. The analysis revealed that the total ear mass and grain mass of wheat plants bearing two or more ears is almost the double than that of the single-spiked plants. It was a further basic experience that the largest ear of ”multiple-spiked” plants is always heavier than the single ear of one-spiked plants. Plants with intense tillering and more ears demonstrate the importance of proper seedbed preparation and drilling and the significance of sowing good quality seeds. These are the factors that determine field germination and emergence, influence the speed and intensity of initial development and by all these factors the sufficient productive tillering. The authors emphasize the use of exact and objective methods at crop estimation, e.g. the relationship between the ear mass and the yield which is in strong correlation whilst ear length and grain mass are not suitable for a precise estimation. The authors conclude that crop estimation and yield analysis must be inevitable tools of modern crop production and will be particularly important in precision agriculture. These tools also qualify the job done by farmers and helps to identify the areas that require special attention.
-
Characteristics of Corn Seed Sales in Hungary
78-81Views:174As seen on the above tables we can acknowledge the fact that the Hungarian corn seed market is very unique and, because of its size, indispensable for all multinational breeder companies. We can also assume that the main places in the market are taken by big companies as Pioneer, with its unbreakable first place, followed closely by Syngenta Seeds and the fusionized Monsanto. Although the Hungarian breeders’ hibrids perform on field tests and trials similarly or better than other varieties developed by the above mentioned companies, we can’t find them even near to those leading few given mostly by Pioneer, Syngenta and Monsanto hibrids. This fact leaves us with a conclusion that the Hungarian companies should change the strategy of their marketing procedures to increase sales.Thus, they need better technology to keep up with their competitors, more advertisement, other marketing „hints”, which can only be fulfilled with a big economical background, unfortunately not yet available for our breeders.
-
The effect of plant density to the yield results and the yield components of maize hybrids
89-93Views:224Maize is the crop that is produced on the second largest area in our country, in Hungary. It is planted on nearly 25% of the country’s growing area and it was produced on 1 090 439 hectares in 2016. Despite the continuous development of the biological basis and production
technology, the growth of the yield results is not constant, its fluctuation is significant. It can be even up to 60%, because of the extremity of the years. The exploitation of the yield potential of modern hybrids is possible if we harmonize the effects of the ecological factors and properly applied instruments of agro technology and by these we ensure their interaction to reach a favorable outcome. The applied plant density is an important, well researched, but at industrial level a not enough utilized element of the maize production.
The results of the extensive tests, done between 2009 and 2015, showed that the genotype, the year effect and the plant density are in strong correlation with each other determining the yield results. In the past seven years the examined genotypes reached the highest yield
performance at the highest plant densities. The early hybrids (RM90–95, FAO 200–300) are capable of producing them at higher plant density, while in case of the mid and late maturity varieties the further increasing of the density after reaching the optimum level led to yield depression.
According to our experimental results, the yield is in close positive correlation with the increase of the plant density. The effect of the growing season has great significance in forming the yield results and this determines the applicable plant density too.
The yield of maize is determined by a resultant of components. The main component is the number of ears per plant and the amount of kernels per ear, which is calculated from the number of kernels on an ear and the weight of them. The number of the kernels on an ear is
calculated from the number of rows on the cob multiplied by the number of seeds in one row on the cob. In dry years, at lower yield levels the yield decreases because of the shorter ears, while at the higher levels the number of kernels in a row and the thousand-kernel weight decreases,causing yield depression this way. From our examinations it turned out that the plant density reaction of a genotype is individual, every variety reaches its maximum kernel number per hectare – in other words the maximum yield - in an individual way. -
Evaluation of the Green bean variety candidate BU-16
75-81Views:150This study presents the results of the variety comparison trials conducted with the French bean variety candidate BU-16 on sandy soil, during two years at the Research Center of the University of Debrecen.
The outstanding characteristics of this variety candidate are the early maturity, the showy pods (yellow, straight, cylindrical cross-section „pencil bean”) and the high yield potential.
The experiments were conducted with a randomized design, with five varieties and four replications. The varieties (of which two are foreign and three are domestic) were as follows: BU-16 fj, Maxidor, Paridor, Hungold and Budai piaci. The individual plots were 2 m wide and 4 m long. The seeds were sown at a density of 30 germs per m2, with a row spacing of 50 cm.
The following parameters were taken: time of flowering, time of green maturity, plant height, height of the lowest pod above ground level, number of pods per plant, distribution of the pods by the state of development (20 plants), usable pod length (1 kg of standard crop-yield), yield per hectare based on the number of plants per plot. The experiments were evaluated statistically with Excel and SPSS softwares.
Results obtained with the variety candidate BU-16:
– The time from the emergence to green maturity is 45 to 46 days, which is short as compared with the other cultivars studied.
– Plant height is 38cm (two years average), which is among the highest ones, together with Paridor.
– The pods are set high above the ground level similarly to Paridor, the height of the lowest pod is 19 cm.
– The average number of pods per plant is 13 (two years average) similarly to Paridor and to Maxidor.
– The distribution of pod size in 2007 is as follows: 68% of the pods of BU-16 is of the standars size, which is a good rate. The value of this trait for the other varieties is 60 to 63%, except for Paridor, the value of which is similar to that of BU-16.
– The usable pod length (10 to 14 cm) is 63% in 2007 and 84% in 2006. Similar pod length rates were obtained for Maxidor, while the rate for Paridor is as high as 91%.
– The total yield and the standard yield of BU-16 is among the highest ones.
According to the results obtained and presented above, the variety candidate BU-16 is the earliest maturing among the varieties tested with high yield potential which, however, is not significantly different from that of the others. Significant differences can only be detected in plant height and the number of pods per plant. Considering the results, BU-16 is to be further tested. -
SNP analysis of ITS1-5.8S-ITS2 rDNA loci in modern and ancient melons (Cucumis melo)
120-124Views:241ITS (internal tanscribed spacer) profiles of the aDNA (ancient DNA) of seed remains extracted from an extinct sample recovered from the 15th century (Budapest, Hungary) were compared to 31 modern melon cultivars and landraces. An aseptic incubation followed by ITS analysis was used to exclude the exogenously and endogenously contaminated (Aspergillus) medieval seeds and to detect SNPs in ITS1-5.8S-ITS2 region of rDNA (ribosomal DNA). SNPs were observed at the 94–95 bp (GC to either RC, RS or AG) of ITS1; and at 414 bp (A-to-T substitution), 470 bp (T to Y or C), 610 bp (A to R or G) of ITS2. The results facilitate the final aim of molecular and morphological reconstructions of ancient melon tpyes.
-
The Role of Trichoderma in the Rhizosphere of Tomato Plants
67-69Views:125It is well established that microorganisms are closely associated with the roots of plants can directly influence plant growth and development. Species of Trichoderma, on the other hand are primarily studied for their ability to control plant disease. The ability of species of Trichoderma to directly promote or inhibit plant growth has been noted for many years.
Tomatoes were treated with different Trichoderma strains by seed treatment and soil inoculation. The Trichoderma were grown on malt-agar medium and conidia were washed off by sterile water for making suspension which contained 107 CFU/ml (colony forming unit/ml). The suspension was used for seed treatment and for the soil inoculation by watering as well. The artificial soil inoculation was made by Trichodermas growing on grounded maize were mixed in mould and tomato seed were sown in it. Tomato seeds were also sown in bags made of close-meshed material which allowed the soil microorganisms to colonize the roots but it simultaneously protected the roots from soil contamination. Roots were put on Trichoderma selective medium to check the root colonization of the Trichoderma.
The tomato plants were bedded out in a field in four repetition. After harvesting by hand the results supported by statistics shown that there was significant differences between the yield of the untreated and treated tomato plants by Trichoderma strains. -
The effects of spraying with onion and garlic extracts on the growth of the plant Anethum graveolens L. and its antioxidant effectiveness
19-24Views:243This experiment was conducted during the 2022–2023 agricultural season in a mixed clay soil at one of the fields of the Faculty of Agriculture, Basra University. The aim of the experiment was to investigate the effect of spraying dill plants (Anethum graveolens L) with aqueous extracts of onion and garlic on their growth. The plants were sprayed with garlic aqueous extract at three concentrations (0, 20, 30 g L-1) and onion aqueous extract at three concentrations (0, 15, 25 g L-1), with three applications at a 15-day interval between each spray. The experiment was designed using a Randomized Complete Block Design (RCBD).
The obtained results showed that the plants sprayed with garlic aqueous extract exhibited significant improvements in most characteristics, except for the fruit content of total dissolved carbohydrates and the yield of fruits from volatile oil. Specifically, the plants sprayed with a concentration of 30 g L-1 outperformed the other two concentrations in terms of plant height (cm), number of branches per plant, and 1000-seed weight for each experimental unit. On the other hand, the plants sprayed with a concentration of 20 g L-1 significantly outperformed in terms of floral inflorescence number per plant and seed yield. Additionally, the plants sprayed with a concentration of 30 g L-1 had the highest chlorophyll content and antioxidant effectiveness of dill leaves (%). However, spraying with garlic extract did not have a significant effect on the fruit content of total dissolved carbohydrates and the yield of fruits from volatile oil.
Regarding the plants sprayed with onion extract, they showed overall superiority in most traits, except for the chlorophyll content of the leaves. Specifically, the plants sprayed with a concentration of 15 g L-1 significantly excelled in plant height, while the plants sprayed with a concentration of 25 g L-1 outperformed in terms of floral inflorescence number per plant and seed yield. The plants sprayed with a concentration of 25 g L-1 also had the highest content of total dissolved carbohydrates in fruits and the highest antioxidant effectiveness of dill leaves and seeds (%). However, the plants sprayed with a concentration of 15 g L-1 had the highest yield of volatile oil. The interaction between the two factors (garlic and onion extracts) had a significant effect on all studied traits, except for the chlorophyll content of the leaves.
-
Research on the allelopathic effect among the species Tanacetum vulgare and some agricultural crops
105-109Views:229The research presents the results of the allelophatic effect upon the germination and growth of plants, immediately after springing, viewed as the interaction between the species of Tanacetum vulgare sin Chrysanthemum vulgare and three other crops: wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), rape (Brassica napus ssp. oleifera L.). The experiments that were performed consisted in applying treatments with aqueous extracts obtained from the roots, leaves, stems and flowers harvested from T. vulgare plants upon the seeds of the three agricultural crops mentioned before. In all aqueous extracts, the results indicate the presence of some chemical compounds that have inhibiting allelophatic effect. The plants upon which tests were made showed great sensitivity, the results on their germination and growth being significantly negative and highly significantly negative.
The conclusions of this research sustain the idea of setting new research objectives in order to discover the chemical compounds from T. vulgare extracts that have such a stong effect and the possibilities they offer. -
Comparison of the physiological responses of an oil-pumpkin cultivar and hybrid under different nitrogen supply conditions
65-69Views:420Cucurbita pepo var. styriaca is known as a medicinal crop among other cucurbits. The benefits of cucurbits fruits are very important in terms of human health, purification of blood, removal of constipation, digestion and supplying energy. The mutant styrian oil-pumpkin (Cucurbita pepo L. convar. Pepo var. styriaca Greb.) exposes a complete lack of lignification of the seed testa. For this reason, this kind of naked pumpkin seed is more consumable as snack than other seeds. The vegetative growth of plants is crucial for fruit production. Nitrogen is the most imperative element for the proper vegetative growth and development of plants which significantly increases and enhances yield and its quality by playing a vital role in the biochemical and physiological functions of plants.
Field studies were conducted to study the effect of nitrogen nutrition on certain physiological parameters and their differences between the styrian oil pumpkin hybrid and cultivar (Gleisdorfi Classic cultivar and GL Rustical hybrid). The small block experiment was carried out based on a factorial experiment with a completely randomised block design and four replications in the Research Institute of Nyíregyháza during the summer period of 2017 and 2018. Three different concentrations of nitrogen fertiliser was applied at sowing. Photosynthetic pigments (chlorophyll-a, -b and carotenoids) were analysed as one of the basic factor of efficient photosynthesis. The yields of two genotypes were also measured in all treatments as seed-weight. The contents of photosynthetic pigments were higher in the oil-pumpkin hybrid, mainly the carotenoids in 2018. The vegetation period of 2018 was drier than in 2017, the hybrid oil-pupmkin was more efficient in stress tolerance than the cultivar with higher carotenoids pool. The obtained yield was more pronounced in the oil-pumpkin hybrid than the cultivar in all examined years. The hybrid GL Rustical was more sensitive to nitrogen treatment than the cultivar Gleisdorfi Classic.
-
Sunflower in Romanian agriculture
153-163Views:320Romania is one of the largest producers of sunflower closely followed by Bulgaria, Hungary, France and Spain. The total sunflower production increased after 1990, due the increase of cultivated surface during the moderate yield average increasing rate. Last year, Romania was ranked first in the European Union (EU) for the area cultivated with sunflower and the total production of sunflower seeds, according to the National Statistics Institute (INS). Romania's position on the map of sunflower producers area is supported by about 1 million hectares sown. In the segment of sunflower productivity, Romania has an unfavourable position, situated in the last places in the classification of the EU's most productive agricultures. In order to increase production per hectare, it is required to perform measures aimed at the elements of sustainable cultivation technology, including the stability of the production level by ensuring phytosanitary protection.
-
Sequence stability at SSR, ISSR and mtDNA loci of common millet (Panicum miliaceum) from the middle ages
10-19Views:259Seed remains of medieval millet, recovered from a 15th century layer (King’s Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval c. millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAPS and mtDNA. Analyses of fragments and sequences revealed
polymorphism at seven ISSR loci (22 alleles) and at the 5S-18S rDNA locus of mtDNA. CAPS analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed
consensus sequence in the medieval sample compared to a modern variety. Morphological characterization of twenty common millet (Panicum miliaceum L., 2n=4×=36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. In the comparative AFLP, SSR and mtDNA analysis modern millet cv. ‘Topáz’ was used. AFLP analysis revealed that extensive DNA degradation had occurred in the 4th CENT. ancient millet resulting in only 2 (1.2%) AFLP fragments (98.8% degradation),
compared to the 15th CENT. medieval millet with 158 (40%) fragments (60% degradation) and modern millet cv. ‘Topáz’ with 264 fragments (100%). Eight AFLP fragments were sequenced after reamplification and cloning. Microsatellite (SSR) analysis at the nuclear gln4, sh1, rps28 and rps15 loci of the medieval DNA revealed one SNP (single nucleotide polymorphism) at the 29th position (A to G) of rps28 locus compared to modern millet.
Mitochondrial (mtDNA) fragment (MboI) amplified at the 5S-18S-rDNA locus in the medieval millet showed no molecular changes compared to modern millet. The results underline the significance of survived aDNA extraction and analysis of excavated seeds for comparative analysis and molecular reconstruction of ancient and extinct plant genotypes. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje.