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Changes of some soil chemical and microbiological characteristics in a long-term fertilization experiment in Hungary
253-265Views:330Agricultural management practices – directly or indirectly – influence soil properties.
Fertilization rates and crop rotation can strongly affect soil pH, soil nutrient supply and soil organic matter content due to the changes of microbial processes. The objective of this study was to compare the effects of different fertilization doses in monoculture and tri-culture of maize (monoculture: only maize grown since 1983, tri-culture: it is a three-year crop rotation system: pea – winter wheat – maize) on selected soil characteristics. The long-term fertilization experiments were set up in 1983 in Eastern Hungary. These experiments are situated west of Debrecen in Hajdúság loess region, on calcareous chernozem (according to WRB: Chernozems).
The test plant was maize (Zea mays L.). One-one pilot blocks were selected from monoculture and tri-culture of the long-term experiments. The observed soil samples were taken in the 30th year of the experiment, in 2013. The doses of NPK fertilizers increased parallel together, so the effects of N-, P- and K-fertilizers cannot be separated.
With the increasing fertilizer doses, the soil pH has decreased in both crop production systems and, in parallel, the hydrolytic acidity has significantly increased. A close negative correlation was proved between the pHH2O, pHKCl and hydrolytic acidity. An increased nutrient content in soil was recorded in every NPK treatment and the available phosphorus and nitrate content increased in higher proportion than that of potassium. Of the measured parameters of C-and N-cycles, fertilization has mostly had a positive effect on the microbial activity of soils. Besides the effects of fertilizer doses, correlation were looked for between soil microbiological properties. Evaluating the ratios among the measured parameters (organic carbon and microbial biomass carbon, OC/MBC ratio; carbon-dioxide and microbial biomass carbon; CO2/MBC proportion), the fertilization rate seems to be favoured by the increase of amounts of organic compounds
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Risk and Risk Management in the Hungarian Sheep Sector
25-29Views:78The aim of this paper is to give an overview of the risk attitudes of Hungarian sheep producers regarding the changes they have had to go through since the political changes of 1989-1990. Moreover, the objective of this study is to strengthen the empirical basis for risk analysis by identifying the importance of farmers’ risk attitudes. The results of a nationwide survey of over 500 sheep farmers presented a framework of risk attitudes, risk sources and applied risk management techniques of livestock producers.
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Effect and interaction of crop management factors and crop year on the yield of maize (Zea mays L.)
31-41Views:221The aim of this study was to determine the combination of treatment levels of crop management factors which can optimize and sustain maize yield under varying climatic conditions. The effect of winter wheat forecrop, three tillage systems (Mouldboard-MT, Strip-ST, Ripper-RT), two planting densities (60,000 & 80,000 plants ha-1), three fertilizer levels (N0-control, N80, N160 kg ha-1) with four replications in irrigated and non-irrigated treatments were evaluated over a five year period, 2015–2019. The obtained results revealed that growing season rainfall positively correlated with yield, whereas, temperature negatively correlated with yield. Impact of adverse weather on yield was less severe in biculture, irrigated plots, at lower planting density (60,000), lower fertilizer rate (N80) and in RT and ST, compared to MT. In years with favorable rainfall, yields of MT and RT were significantly (P<0.05) higher than ST. However, in a less favorable year, such as 2015, with 299 mm growing season rainfall and the lowest July rainfall (59% below mean) there was no significant difference (P>0.05) in yield among the three tillage treatments. Higher planting density (80,000), and fertilization rate (N160) in tandem with MT are treatments combination conducive for high yield under favorable climatic conditions, whereas, in years with low rainfall and high temperatures, RT and ST offer alternative to MT for optimum yield with 60,000 plants ha-1 and N80 treatment level. Crop year effect accounted for 20.7% of yield variance, fertilization 35.8%, forecrop 12.8%, plant density 3.4%, tillage 1.2% and irrigation <1%. It is conclusive that with proper selection of the appropriate levels of agrotechnological inputs the adverse effect of weather on yield can be mitigated.
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Biological control of sweet chestnut on Pécsbánya, Hungary
77-81Views:252The supervision of plant hygiene of sweet chestnut grove on Pécsbánya (South Hungary) started more than four years ago. Hypovirulent strains were applied as a biological process to control Cryphonectria parasitica fungus which causes the chestnut blight disease. By now the performed interventions have shown obvious results, the vitality of the trees has greatly improved, the amount of harvested nuts is increasing, and the hypovirulent strain has been spreading within the area. During plant health inspection the galls of chestnut gall wasp (Dryocosmus kuriphilus) was found in the year of 2015, which is the obvious symptom of new occurrence of the pest. The pest was eradicated by destroy galls, which allows taking out of consideration the damage by now in this area.
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Agricultural aspects in river basin management plans
149-152Views:104In the European Region agriculture is the second largest water user after power industry cooling water use. As part of the implementation of Water Framework Directive EU Member States prepared their river basin management plans by the end of 2009 or first half of 2010, In these plans impacts of agriculture on water bodies have received attention. The detailed information elaborated in the plans by countries and river basin districts were uploaded into the WFD section of the WISE system. This database provides opportunity for multi-criteria analysis for different water types. The paper discusses the effects of agriculture on hydromorphological pressures and impacts affecting surface water bodies. It was pointed out that among the pressures affecting European surface water bodies the hydromorphological and diffuse pressures represent the highest ratios (Figure 1). Within the hydromorphological pressures affecting classified surface water bodies the ratio of pressures related to agricultural activities is low,
it does not exceed 1% at European level. In case of Hungary the agriculture related river management pressures effect about 80% of the surface water bodies, which is much higher than the corresponding European average. The agricultural water abstractions affect about 10% of the Hungarian surface water bodies (Figures 2 and 3). The river and lake water bodies are impacted in significant ratio by nutrient enrichments and organic material enrichments, while in case of river water bodies the impact of organic material enrichments is also significant (Figures 4 and 5). -
Microbiological quality of bulk tank raw milk from two dairy farms in Hajdú-Bihar County, Hungary
105-112Views:327Two main channels have been identified to be responsible for microbiological contamination of raw milk and milk products. Firstly, contamination has occurred due to udder infection from the cow or the blood which harbours most bacteria that come in contact with the raw milk. Secondly, via external factors (may include faeces, skin, contaminated water, environment etc.) which are associated with the operation of milking. There is direct contact with the milk and/or surfaces before, during or after the milking, posing public health risk and economic decline. The aim of this study was to examine the bacteriological quality of bulk tank raw milk samples collected from two different size dairy farms (Farm 1 and Farm 2) of different housing forms (cubicle loose and deep litter) in Hajdú-Bihar County, Hungary in July, 2017. Three samples were taken from each farm, and the total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, and yeast and mould count were determined in them.
The results clearly showed low level of all measured bacteria group load in Farm 1 samples in comparison to Farm 2 with the exception of coagulase-negative Staphylococcus (CNS) which represented high level in general, indicating significant difference (P<0.05). The mean value of total plate count in Farm 2 samples was higher (1.0 × 105 CFU/mL) than Farm 1 samples (2.8 × 104 CFU/mL). There was a significant difference (P<0.05) in mean count of coliforms in raw milk samples between Farm 1 and Farm 2. Similarly, results of E. coli were significantly different (P<0.05) with mean count of 1.44 × 102 CFU/mL and 2.02 × 103 CFU/mL for Farm 1 and Farm 2 respectively.
Results of Staphylococcus aureus also showed significant difference (P<0.05) with mean count of 9.7 × 101 CFU/mL for Farm 1 and 6.28 × 102 CFU/mL for Farm 2. The mean of mould count recorded was 1.07 × 102 CFU/mL and 4.93 × 102 CFU/mL for Farm 1 and Farm 2 respectively. The recorded mean of yeast count was 1.68 × 103 CFU/mL and 3.41 × 103 CFU/mL for Farm 1 and Farm 2 respectively; however, both farms showed no significant difference (P>0.05) in terms of mean of mould and yeast count. Although Farm 2 produced six times lower milk quantity than Farm 1, the measured microbial parameters were high. Both farms’ microbiological numbers were higher above the permitted limit values as stated by Regulation (EC) No 853/2004, Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.).
This could be an indication of non-conformance to effective GMP, ineffective pre–milking disinfection or udder preparation, poor handling and storage practice, time and temperature abuse and inadequate Food Safety Management System Implementation. Therefore, our recommendation is as follows; establish control measures for pre- and postharvest activities involved in the milking process which would be an effective approach to reduce contamination of the raw milk by pathogenic microorganisms from these farms, strict sanitation regime and hygiene protocol be employed and applied to cows, all equipment, contact surfaces and minimize handling of the milk prior, during and after milking. This will also serve as scientific information to the producers for continual improvement in their operations.
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Application of laparoscopic artificial insemination in conventional Lacaune sheep farm using frozen-thawed semen
133-138Views:320The complex anatomical structure of the ewe reproductive tract accompanied with low quality of frozen ram semen for artificial insemination, resulted in a challenge with regard to using superior genotypes for reproductive ovine performance. Hence, improved genetics in ovine management has not been efficiently and widely used especially in undeveloped countries. Therefore, intrauterine semen deposition by laparoscopic insemination should be adopted in the current sheep production systems. Thus, this study aimed to assess the pregnancy rate and lambing rate of ewe inseminated by laparoscopic insemination techniques using frozen-thawed semen. The research used imported frozen semen from two rams of the Lacaune breed. Ewes were grouped according to age in years (1, 2 and 4). Before insemination, the semen was examined microscopically for its motility and viability and thereafter the laparoscopic artificial insemination technique was performed to 19 Lacaune breed ewes using frozen-thawed semen. The overall pregnancy and prolificacy rates were 31.57% and 42.10% respectively. Out of 2 ewes in the 1-year age group that were inseminated, only 1 ewe lambed representing 50%. However, from 16 ewes inseminated of four-year age group, 5 ewes lambed representing 31.25%. Significant difference based on age group was not evaluated due disproportionate of the data, (such that the data included 2 ewes in one-year-old age, 1 ewe in 2-year-old age and 16 ewes in 4-year-old age). Based on the ram semen, 33.33% and 30% of the inseminated ewes were pregnant from ram A and ram B semen respectively. However, in the case of prolificacy rate, 44.44% and 40 % of the ewes lambed from using semen of ram A and B, respectively. There was no significant difference (p>0.05) in pregnancy and prolificacy rates based on semen from the two rams. In conclusion, in this research study, ram semen had no significant effect on pregnancy and prolificacy rates using laparoscopic AI on Lacaune sheep. This could be due to the fact that the rams had very good quality semen. Evaluation of ram semen, accompanied with appropriate ewe selection based on age and rightful deposition of semen could lead to better and more consistent results. Overall this could contribute to the successful application of laparoscopic artificial insemination in Lacaune sheep production systems for enhanced productivity.
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Determining the optimal age of breeding gilts and its impact on lifetime performance, Literature review
15-20Views:555Lifetime sow performance is a critical indicator for producers managing a commercial sow herd. It is comprised of sow longevity, lifetime piglets born alive, lifetime number of pigs weaned and lifetime non-productive sow days. Increased lifetime performance and longevity in sows reduces costs of replacement gilts and improves herd performance and profitability (Sasaki et al., 2008, 2011). One of the most critical factors driving the performance of sow herds is gilt management. Decisions regarding gilts have profound effects on sows’ lifetime performance. Age at first breeding of a gilt is a management decision that has been shown to affect performance and retention of the gilt in a herd.
The main aim of this review is to establish factors affecting age of first breeding of gilts and its impact on lifetime performance.
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Practical experiences in Moodle LMS
129-133Views:86We use Moodle at the University of Debrecen, Business- and Agricultural Department since January 2007. Moodle is an open source Learning Management System.
Learning Management System (or LMS) is a software package that enables the management and delivery of learning content and resources to students. Most LMS systems are web-based to facilitate "anytime, anywhere" access to learning content and administration. LMS tracks student progress in a course and indicates completions. At the least, learning management systems track individual student progress, record scores of quizzes and tests within an online learning program, and track course completions.
Moodle has more and more function at our Department in education. At present, we work to introduce Moodle in our Faculty. Therefore, we took lessons for the tutors about the usage of the Moodle. Our aim to develop such a learning system, which is an integral part of educational process. -
The time, than the resource of the managerial activity
19-24Views:79The role of the time factor in management is increasing. Change directs attention to the time factor from the point of view of the organisation, and management does the same from that of
the person. I examined the time management practice of managers of organisations related to agribusiness, and the reaction time characterising the adaption ability of the organisations through a questionnaire survey. I analysed the collected data with scientifically established statistical methods.
During studying resources I analysed the time factor from two aspects. On the one hand, I wanted to know what role time has in managers’ opinions in increasing company efficiency and
performance. On the other hand, I asked them how much they consider this resource to be expandable. -
Comparative study of special honey products and herbhoneys
117-120Views:391Honey has a positive effect on human body due to its high content of biologically active substances (e.g. monosaccharides, vitamins, enzymes, amino acids, polyphenolic compounds). The properties of honey depend on its botanical origin due to the bioactive plant components, mainly secondary metabolites that are included in honey made by bees from nectar. Herbally infused honeys are delicious products that combine the therapeutic action of herbs and honey. Additionally, herbs can provide nutrient fortified syrup for honey bees and protect them against diseases and other ecological threats.The aim of this study was to define the physicochemical properties of multifloral honey, herb enriched natural honey and herbhoney samples. We measured the moisture content, pH value, electrical conductivity and proline content. Although great diversity was observed in the basic properties of the examined products. In our study, we found that the electrical conductivity shows the significant differ between the groups. All the samples, including the herbhoneys passes the quality standards of honeys. -
Application of semen evaluation techniques
5-11Views:232Laboratory methods of semen evaluation are used to select males for artificial insemination. The current review describes several techniques that have been recently used for sperm analysis. Conventional microscopic methods in combination with the objective computerassisted sperm motility and morphology analyzers and flow cytometry, allows to obtain more precise information about the membrane and functional status of spermatozoa. By using several methods to detect motility, viability, acrosomal and capacitation status besides DNA integrity sperm biology and some of the mechanism involved in sperm cry injury can be better understood. The number of possible targets related to sperm quality is increasing, and possible that some of them could enable sperm analysis for predicting freezability and fertility to be improved.
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A new model for predicting carbon storage dynamics and emissions related to the waste management of wood products: introduction of the HWP-RIAL model
75-81Views:154The ability of wood products to store carbon allows for their significant contribution to the climate mitigation efforts and the emission reduction commitments set by the EU. In order to optimise the carbon storage capacity of wood products, it is important to take climate mitigation aspects into consideration as much as possible during their production, use and waste management. The aim of this study was to quantify the effects of product development, recycling, and waste management technologies on carbon storage and emissions. In the frame of the ForestLab project, a new model and decision support tool was developed, which is able to predict the duration of carbon storage of wood products and the evolution of emissions from them. The developed HWP-RIAL model (Harvested Wood Product Recycling, Incineration And Landfill model) uses the methodology of the Intergovernmental Panel on Climate Change (IPCC) to calculate emissions, which is also used in the National Greenhouse Gas Inventory report. It combines the IPCC waste model with equations describing the carbon storage and emissions of wood products, and the model is also supplemented with a self-developed recycling and waste routing module. This paper provides insight into the operation of the model by following the life cycle of 200,000 m3 particle board.
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The Role and Significance of Soil Analyses in Plant Nutrition and Environmental Protection
3-8Views:98Hungary has a rich history of soil analyses and soil mapping. Our main tasks today are the preservation of soil fertility as well as balancing the goals of production and environmental protection. The main requirement of agricultural production is to adapt to ecological and economic conditions.
In a series of consultative meetings in the past seven years, representatives from Central and Eastern Europe have analyzed nutrient management practices in their respective countries. According to a joint memorandum agreed upon in 2000, in the countries awaiting accession, the quantity of nutrients used per hectare is considerably smaller than the Western-European usage targeted through special subsidies. The current low nutrient usage contradicts the principles of sustainability and that of the efficient use of resources, jeopardizing soil fertility.
In Hungary, the use of inorganic fertilizers underwent a dynamic development, which manifested itself in an almost tenfold usage growth between 1960 and 1985. This growth slowed down somewhat between 1985 and 1990 and then reduced dramatically after 1990, reaching record lows at the usage levels of the 60s. The nutrient supply has had a negative balance for the last 15 years.
The increasing and then decreasing usage trends can equally be detected in the domestic yield averages of wheat and corn as well as in the nutrient supply of soils. Yields were the largest when usage levels were the highest, and decreased thereafter. Draughts have also contributed to smaller yields. The dramatic decrease in the use of inorganic fertilizers when adequate organic fertilizers are lacking endangers our soils’ fertility.
About 50% of soils in Hungary are acidic. Acidity is mostly determined by soil formation, but especially on soils with a low buffering capacity, this acidity may intensify due to inorganic fertilizers. Sustainable agriculture requires the chemical improvement of acidic soils. According to their y1 values, the majority of our acidic soils need to be improved. This chemical soil remediation is required in 15% of the acidic soils, while it’s recommended for another 20% of these soils.
Results of the analyses conducted in the framework of the soil-monitoring system set up in Hungary in 1992 show that in 95% of the analyzed samples, the toxic element content is below the allowable limit. Cultivated areas are not contaminated; toxicity above the legal level was found only in specific high-risk sampling areas: in the vicinity of industry, due to local overload. The basic principle of sustainable agriculture is to preserve soil fertility without undue strain on the environment. The intensity of the production needs to be considered according to the conditions of the site; i.e.; nutrient management needs to be site-specific. It is recommended to differentiate three types of cultivated land in terms of environmental sensitivity: areas with favorable conditions, endangered areas, and protected areas, and then to adopt nutrient management practices accordingly. To meet all the above-mentioned goals is impossible without systematic soil analysis. Tests conducted by the national monitoring system cannot replace regular field measurements. -
The possibilities and framework of conditions of organisational development at a particular administrative body
63-70Views:121The demand for the recognition, identification and introduction of measures aimed at improving organizational culture and the efficiency of management and leadership plays a crucial role in the life of every organization, including the law enforcement bodies as well. The tasks and responsibilities of the Hungarian Police Force have changed considerably both in terms of quantity and quality since Hungary’s accession into the European Union first, and since the country’s joining the Schengen Zone. We are now facing problems formerly unheard of (e. g. the integration of the police force and the border guard, joining the Schengen Zone, the emergence of new forms of crime). As border control within the Union stopped, organized crime has become more powerful. The changes are by no means over: in order to improve the subjective sense of security of the population and to advance organizational efficiency, a number of changes are being implemented at present. For the internal security of the member states of the Union and for the improvement of the efficiency of police work in the member states, the use of consciously coordinated management methods is indispensable. These efforts are the subject of this essay.
A police force is an partially open system, the work of which is influenced by a number of external processes and factors. The organizational culture of a police force is determined by its traditions on the one hand, and by the demands to develop its functions as an authority and a service provider on the other. It is undeniable that any conscious effort in shaping and developing the organizational culture of the police requires special competences and commitment from the leaders of the organization. Furthermore, it is also necessary to modernize the management tasks. It applies to all the levels of leadership, from the head of a patrol to the supreme commander of the police force.
The organizational scheme of the police force is that of a functional, linear system. Such an organization is characterized by a relatively high degree of inflexibility, a slow and cumbersome reaction to external changes. All these may adversely influence organizational efficiency. It therefore appears to be necessary to examine the present structure of police, and survey the possibilities of modernization. Modernization does not necessarily mean a change in the structure; it may be restricted to changes in organizational culture, the creation of an atmosphere favourable for reforms, and the coordination of diversified structures. In this paper I wish to demonstrate some of the possibilities of improving organizational culture and organization development, based upon the findings of a specific survey.
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Examinations of the carbon dioxide emission of the soil in the case of different tillage methods in a field experiment
209-212Views:243Today's global challenge is the increasing concentration of carbon dioxide (CO2) and other greenhouse gases in the air. The level of CO2 emissions may be significantly affected by the agriculture and, more specifically, the applied tillage method, even though to a lesser extent than industrial production. On a global scale, the CO2 emission of an agricultural area is insignificant in comparison to that of a large-scale plant in an area of the same size, but areas under cultivation, including arable land, have a large global area. In this paper, we investigated the relationship between applied soil tillage methods and carbon dioxide emissions in the case of different fertiliser treatments. In our experiment we examined four types of tillage with five different fertiliser effects. Comparing fertiliser treatments and tillage methods, it was found that their interaction significantly affected carbon dioxide emissions, the lowest value was obtained in the case of the 210 l ha-1 Nitrosol+N-LOCK – tillage radish treatment. Strip and tillage radish methods have relatively homogeneous, low value. -
Improved soil and tomato quality by some biofertilizer products
93-105Views:283The use of microbial inoculums is a part of sustainable agricultural practices. Among various bioeffectors, the phosphorus-mobilizing bacteria are frequently used.
The objective of this study is to investigate the effect of some industrial biofertilizer inoculums, of containing P-mobilizing bacteria on the quantity and some quality parameters of tomato fruits. Spore-forming industrial Bacillus amyloliquefaciens FZB42 (Rhizovital) as single inoculums and combinations with other Bacillus strains (Biorex) were applied on Solanum lycopersicon Mill. var. Mobil test plant. Soil microbial counts, phosphorus availability, yield and fruit quality, such as total soluble solids (TSS) content and sugars (glucose, fructose) were assessed. The results found that single industrial inoculums of FZB42 product had positive effect on P-availability and fruit quality in the pots. Fruit quality parameters, TSS content, soluble sugars were significantly improved (p<0.05). Such better fruit taste was correlated significantly by the most probable number (MPN) microbial counts. Use of such bioeffector products is supported by the positive interrelation among measured soil characteristics and inside healthy quality parameters of tomato fruits.
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Sustainable energy management – the importence of the renewable energy sources, their difficulties and chances
145-154Views:108The biggest question of the century standing before us is that if people will be able to direct the development to sustainable direction. One of the components of global problems threatening
us is the pressure that the energy management puts on the environment, which can be significantly lowered by utilizing renewable energy sources. The aim of my study is to draft the
model of a sustainable energy management, putting the renewable energy sources into it in a sensible way, regarding the possibilities of Hungary. -
Management of phytopathogens by application of green nanobiotechnology: Emerging trends and challenges
15-22Views:325Nanotechnology is highly interdisciplinary and important research area in modern science. The use of nanomaterials offer major advantages due to their unique size, shape and significantly improved physical, chemical, biological and antimicrobial properties. Physicochemical and antimicrobial properties of metal nanoparticles have received much attention of researchers. There are different methods i.e. chemical, physical and biological for synthesis of nanoparticles. Chemical and physical methods have some limitations, and therefore, biological methods are needed to develop environment-friendly synthesis of nanoparticles. Moreover, biological method for the production of nanoparticles is simpler than chemical method as biological agents secrete large amount of enzymes, which reduce metals and can be responsible for the synthesis and capping on nanoparticles.
Biological systems for nanoparticle synthesis include plants, fungi, bacteria, yeasts, and actinomycetes. Many plant species including Opuntia ficus-indica, Azardirachta indica, Lawsonia inermis, Triticum aestivum, Hydrilla verticillata, Citrus medica, Catharanthus roseus, Avena sativa, etc., bacteria, such as Bacillus subtilis, Sulfate-Reducing Bacteria, Pseudomonas stutzeri, Lactobacillus sp., Klebsiella aerogenes, Torulopsis sp., and fungi, like Fusarium spp. Aspergillus spp., Verticillium spp., Saccharomyces cerevisae MKY3, Phoma spp. etc. have been exploited for the synthesis of different nanoparticles. Among all biological systems, fungi have been found to be more efficient system for synthesis of metal nanoparticles as they are easy to grow, produce more biomass and secret many enzymes. We proposed the term myconanotechnology (myco = fungi, nanotechnology = the creation and exploitation of materials in the size range of 1–100 nm). Myconanotechnology is the interface between mycology and nanotechnology, and is an exciting new applied interdisciplinary science that may have considerable potential, partly due to the wide range and diversity of fungi.
Nanotechnology is the promising tool to improve agricultural productivity though delivery of genes and drug molecules to target sites at cellular levels, genetic improvement, and nano-array based gene-technologies for gene expressions in plants and also use of nanoparticles-based gene transfer for breeding of varieties resistant to different pathogens and pests. The nanoparticles like copper (Cu), silver (Ag), titanium (Ti) and chitosan have shown their potential as novel antimicrobials for the management of pathogenic microorganisms affecting agricultural crops. Different experiments confirmed that fungal hyphae and conidial germination of pathogenic fungi are significantly inhibited by copper nanoparticles. The nanotechnologies can be used for the disease detection and also for its management. The progress in development of nano-herbicides, nano-fungicides and nano-pesticides will open up new avenues in the field of management of plant pathogens. The use of different nanoparticles in agriculture will increase productivity of crop. It is the necessity of time to use nanotechnology in agriculture with extensive experimental trials. However, there are challenges particularly the toxicity, which is not a big issue as compared to fungicides and pesticides.
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Reference genes for livestock gene expression profiling – Literature review
81-89Views:195Quantitative real-time polymerase chain reaction (qPCR) is an essential tool for understanding animal cell’s response to developmental progression or to different experimental conditions at gene expression level. However the reliability of this method heavily lies on proper normalization (measuring a target and a reference gene’s expression from the same sample to correct for technical related variations).
Our literature review aimed to summarize the articles addressing the most important livestock species in regards of reference gene stability used as normalizers for quantitative real-time polymerase chain reaction experiments. Stably expressing reference genes were categorized into 14 distinct groups according to gene function. The number of reference genes tested and the publication numbers according to years and the ranking algorithms were also noted.
Counting showed that genes encoding ribosomal protein components are ranked as most stable in majority of cases and therefore should be taking into account for qPCR stable normalizer gene finding experiments. -
Sow removal patterns in commercial breed-wean herds of Midwest, USA
9-14Views:379An observational retrospective study was done to provide up-to-date information on recent sow removal patterns from 9 breed-wean herds of Midwest USA. The study comprised of sow’s removal reasons and removal types of F1 Landrace x Yorkshire gilts entered in the herds between 1st Jan 2014 and 31st July 2016. Data was extracted from existing database on Dec 2018 and 15% of the sows were still active in the herds hence not included in the study. Descriptive statistics showed that out of the 20,009 removed sows, planned removals comprised of farrowing productivity (FP) 3,523 (17.6%) and old age (OA) 1,785 (8.9%) while unplanned removals consisted of reproductive failure (RF) 7,786 (38.9%), health problems (HP) 2,629 (13.1%), locomotion problems (LP) 1,473 (7.4%) and conformation issues (CI) 1,350 (6.8%).‘Did not conceive’ and “No heat” were observed as the main contributing factors accounting for 37.6% and 32.9% respectively for gilts & sows removed by RF.13.5% of the gilts (Parity 0) were removed from the herds before attaining their first litter of which 64.1% of their removals was due to RF. Removal type consisted of slaughter (S) 85.0%, found dead on the farm (DoF) 10.8% and euthanized (E) 4.2%. The research findings depict an upward trend of sow RF removals in the US swine herds posing a serious concern for US swine producers. Characterization and quantification of sow removals gives a revelation on the deeper intrigues about the vulnerability of the various parity in respect to common causes of RF. This helps swine producers to decisively improve on gilt replacement selection, reproductive efficiency, health and nutrition management all aimed at increasing overall swine productivity and efficiency in management. Swine farmers in the US can now focus their efforts towards curbing unnecessary RF removal within parity specifics.
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The study of the fertilizing effect of wheat straw ash in a greenhouse experiment
47-51Views:219The effect of wheat straw ash as a fertlizizer was studied in a pot experiment with an acidic sandy loam soil (pHKCl=4.9) with weak K and P supply. The test plant was ryegrass (Lolium perenne). The treatments were the following: 1. control untreated soil, 2. NPK fertilizer, 3. small dose of ash (1.4 g kg-1), 4 large dose of ash (2.8 g kg-1), 5. small dose ash completed with NP fertilizers. Soil parameters (pHH2O, pHKCl, ammoinum-lactate soluble P, K, 0.01 M CaCl2 soluble PO43--P, K, Mn, Cu, Zn ) and plant parameters (yield, P, K, Ca, Mg, Zn, Mn uptake) were investigated. Based on the analysis of the straw ash sample and the results of pot experiment it can be stated that the wheat straw ash is suitable for the fertilization of the studied soil. The small dose ash completed with NP resulted in the largest yield increment (43%). In order of the treatments the pHKCl changes to: 4.9, 4.8, 5.2, 5.8, 5.1. As the N : P2O5 : K2O ratio is 0 : 1: 3.5 in the wheat straw ash sample, to reach optimal yield ash should be completed with N and P.
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Seasonal variations in somereproductive parameters of Dorper Rams in Hungary
17-20Views:307The purpose of the present study was to test the hypothesis that season has an affect on semen quality and scrotal circumference of Dorper rams. The experiment was carried out with six Dorper rams aged between 15 and 18 month. Semen samples were collected with artificial vagina and volume, concentration (x 109/ml), total sperm number/ejaculate (x 109), mass motility (0–5), progressive motility (%), scrotal circumference (cm) was observed. Significant differences (P<0.05) were observed in concentration, total sperm number/ejaculate, scrotal circumference in different seasons. Volume was the highest in autumn (1.4±0.5 ml) and the lowest in the spring (1.3±0.4 ml). Concentration of semen was lower in spring (2.6±1.5 x 109) and summer (3.3±1.5 x 109) as compared to fall (4.1±1.1 x 109) (P<0.05). Regarding total sperm number/ejaculate (x 109), scrotal circumference (cm) all the seasons differed significantly (P<0.05), although the season had no effect on mass motility and progressive individual motility (P<0.05). In conclusion the present study showed that semen quality parameters and scrotal circumference of Dorper rams were better in autumn than in the other sea- sons.
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The effect of different genotype cattle grazing on the nutrient content of saline grasslands vegetation
46-50Views:208Maintaining saline grasslands in good condition, preserving their yield and diversity is important not only for the purpose of nature conservation, but also for farming. Therefore, the primary purpose of our study is to analyse the effect of pasture use of the smaller weight extensive and the larger weight intensive beef cattle on the grassland vegetation and nutrient content. In this way, we can answer the question whether grazing for nature conservation can be achieved with more profitable, more economical and more intense varieties.
The tests were carried out in May 2016 and May 2017, in the Hortobágy National Park (Pap-ere and Zám puszta), where a total of 16 sample areas were analysed. These areas are grazed with extensive beef cattle (Hungarian grey) and mixed genotype of intensive cattle. The associations were selected along a moisture gradient, such as wet salt marsh (Bolboschoenetum maritimi) and drier saline meadow (Beckmannion eruciformis). All the vegetative material collected both years in May was analysed for the following parameters: dry matter, crude protein, crude fibre and life-sustaining net energy content. We compared the effects of medium grazing (0.46 livestock/ha) and abandonment on vegetation and nutrient content.
We examined the effect of (i) grazing, (ii) different grasslands (salt marsh, saline meadow) and (iii) grazing of different cattle breeds (Hungarian grey, intensive beef) on the nutrient content of the vegetation of grasslands Based on our results, it was found that grazing had an impact on crude protein and life-sustaining net energy content. The highest crude protein content (12.75 m/m%) was obtained in the year 2017 in the area where higher density had been grazed for two years. For the lifesustaining net energy, the highest value (5.05 MJ/kg d.m.) was also obtained in 2017 and the lowest in 2016. Furthermore, it was found that there was no significant difference between the effect of the two cattle breeds on the parameters examined. Significant effects were observed only in the case of life-sustaining net energy: in the area of intensive beef cattle we received a higher value (5.15 MJ/kg body weight) than in the area with extensive beef cattle (4.96 MJ/kg body weight).
Our results have also shown that cattle grazing is of the utmost importance for the maintenance of both wet and mesophilous habitats. Based on our three-year study, we can say that grazing by both extensive and intensive cattle breeds is suitable for the management of saline habitats.
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Examination of compost maturity using reflectance
29-34Views:209Composting is one of the most popular recycling processes for organic waste. Composting plays an important role in waste and by-product management and is becoming increasingly important in both sustainable energy management and circular economy. Composting transforms organic matter to produce a safe and stable by-product (compost) that can be applied to arable land in a similar way to fertilizer. Physical, chemical and biological methods can be used to monitor the process and to determine the maturity of the compost, as spectrometric/spectroscopic methods play an important role in the analysis of different environmental samples.
Our aim was to (1) non-destructively detect the effects of different additive ratios on the spectral properties of the composting process and the spectral data of different compost mixtures, (2) to find the wavelength ranges of the reflectance curve (inflection points) sensitive to compost maturity, (3) to determine the correlation between the inflection points and the chemical and physical parameters measured in compost by conventional methods.
The mixture of broiler and hen manure and zeolite was composted 62 days in windrow composting. In the composting experiment, the moisture content and temperature (°C) were measured every three days and compost samples were taken and in 10% destillated aquaeous suspension were measured the pH and electrical conductivity (mS cm-1). Compost samples dried to mass stability were spectrally analyzed in the wavelength range 400–1000 nm with AvaSpec 2048 spectrometer.
Based on the results, the reflectance of mature compost were smaller in the last days of composting than the reflectance values of day 0 samples, thus compost maturity can be detected spectral in the VIS-NIR wavelength range. For the tested compost prisms, the reflectance of each sampling day shows a constant slope, with a significant overlap of the reflectance curves up to 400–700 nm wavelength range, and there was a breakpoint in the 700–750 nm wavelength range which was proved by binary encoding.