Search
Search Results
-
Comparison of the sample preparation methods worked out for the examination of the element content of wine
77-82Views:428The examination of the potentially toxic elements content of the wines is not easy task, because the most elements are in little concentration (mg kg-1 or μg kg-1) in the wine and the wines contain great amount of organic matrix. The efficient sample preparation is essential for the accurate determination of element content. The eim of our research was to determine which sample preparation method will be the most efficient in examination of wines with ICP technology. The examined wine sample was a 2008 Chardonnay from the Eger wine region. We did the sample preparation and analysis examination in University of Debrecen, Centre for Agricultural and Applied Economic Sciences, Institute of Food Science, Quality Assurance and Microbiology.
We did the analysis examinations with ICP- MS (inductively coupled plasma mass spectroscopy). We always did the sample preparations and the examinations in three times rehearsal. The applied sample preparation methods: dilution with distilled water, open digestion and microwave digestion.
We were able to measure B, Al, Mn, Fe and Zn with only dilution and open sample preparation. In the smaller quantity present Sr and Ba were measurable in the wine in the case of all three methods well. We were able to measure the Co with dilution and open digestion method, while Cr, Ni, and Te with only dilution method. In the case of arsenic we were not able to measure reliable result with dilution and open digestion method because of organic matrix and other components
(alcohols, monosaccharides, polysaccharides, polyalcohols and inorganic salts). On the whole we are able to say that in the case of certain elements (B, Mn, Fe, Zn, Sr, Ba) the open digestion and dilution sample preparation is applicable well, however, in the case of certain elements (As, Al, V, Cr, Se, Mo, Cd, Hg, Pb) we have to develop the methods. It may be development of one of the way, if we develop sample preparation methods to examined element specifically and not
to wine generally. -
The effect of sowing date and plant density of winter oilseed rape (Brassica napus var. napus f. biennis L.) population
213-215Views:521The experiment has been set up in the University of Debrecen Látókép Experimental Station in three different years (2014, 2015 and 2016), three different plant densities 200, 350 and 500 thousand ha-1, four replications of the same nutrient supply with using a line spacing of 45 cm. In the experiment, the fore crop was winter wheat in each year. The amount of weeds was observed five times in the last experimental year (2016/2017). In the three experimental years, the highest yield was harvested from the early sowing plot with the highest plant density. On the basis of the Pearson’s correlation analysis there was significant negative correlation (r=-0.583) between the effect of the annual year and yield of the hybrid.
-
Failure causes and effect analysis of water supply systems
209-214Views:139The present paper intends to overview the problems of the failure causes and effect analysis of water supply systems. The author’s efforts were channeled to systemizing, classifying and adapting the content to the hydraulics engineering field.
-
Simulation of optimizing decisions and risk analysis in investment plans
160-164Views:194Investments always contain risks, as data referring to the future are planned and uncertain. Therefore, besides feasibility analyses we need to perform risk analyses, as well. Through statistic simulation methods, our aim is to examine how uncertain and prospective data as risk factors affect investment-profitability indices. On the other hand, our aim is to find out the optimal innovation – financing decisions by using decision optimizing
methods. -
Food allergy, safety and analytics
323-326Views:201The food allergy is a hypersensitivity reaction against naturally occurring proteins in food. These types of disease can cause not only personal inconvenience to the patient but serious health, food safety and food analysis, social-economic problems. The only effective treatment for these illnesses is a life-long diet avoiding the allergenic foods or components of food. In the interest of the patients’ health 14 allergenic components must be labeled on the food packaging. To meet the requirement of regulation reliable and valid analytical methods are necessary which for the most allergenic foods are not available.
-
Study of the economic involvement of civil organizations
139-145Views:206On the basis of definitions found in the literature, as well as my own, civil organizations are such structures that basically have specific organizational characteristics and their own management tasks in view of their operational principles.In my study, I describe the status quo of civil organizations, as regards their “economic involvement”. On this basis, I examined the level of the economic involvement of civil organizations, more specifically as to the level of economic partnership between representatives of civil organizations and greater society. I placed the economic involvement of civil organizations into three categories: ‘active’, ‘passive’ and ‘has no effect on individuals in society’. During my research, I largely build my investigations on the results I obtain through questionnaires, which I measure on a representative sample. On the basis of this sampling, the County Court of Justice was able to launch its own website. Thus, my research on this topic included the townships in Hajdú-Bihar County.
The results of my research arise from an analysis of data I collected in 2010–2011. This data allowed me to be able to make several observations concerning the specific activities of civil organizations. I have result of the organizations’ annual income, as well as the types of revenues they obtain the county. The results of my analysis of the examined organizations show that more than two-thirds (66.7%) of their available revenues totaled less than one million Hungarian Forints, with no significant difference in the amount of revenue existing between the two organizational types (association, foundation). The most common source of revenue claimed by the studied organizations was from membership fees and/or various types of funding programs. In examining civil organizations on the basis of their type, I made the observation that in the case of associations, it was the membership fees which were the most characteristic type of income, followed by the 1% donation possibility from personal income tax filings for foundations, but funding proposal possibilities were also important for both types of organization.
-
Associations analysis of production traits with leptin gene T3469C polymorphisms in pig
39-43Views:530The aim of this study was to define the connection between the leptin (LEP) gene T3469C polymorphism and its potential association with production traits in improved hybrid pigs. The study included data from 397 gilts and barrows from 2 different sample. The polymorphism was identified by using the polymerase chain reaction-restriction fragment-length polymorphism (PCR–RFLP) method with HinfI restriction enzyme. Two alleles of LEP gene were identified: T (0.93) an C (0.07). The analysis of values of production traits, depending on LEP genotype did not reveal significant (P≤0.05) differences. In the examination the loin diameter (between the 2nd and 3rd ribs), the live weight at slaughter and the averege daily gain during fattening were higher at pigs with C allele than pigs with TT genotipe. Accordingt to our data the effect of C allele was favourable in this population, because these animals had bigger bodyweight without valuable change of lean meat percent.
-
Analysis of sweet corn nutritional values using multivariate statistical methods
103-108Views:659Processing large amounts of data provided by automated analytical equipment requires carefulness. Most mathematical and statistical methods have strict application conditions. Most of these methods are based on eigenvalue calculations and require variables to be correlated in groups. If this condition is not met, the most popular multivariate methods cannot be used. The best procedure for such testing is the Kaiser-Meyer-Olkin test for Sampling Adequacy. Two databases were examined using the KMO test. One of them resulted from the sweet corn measured in the scone of the study, while the other from the 1979 book of János Sváb. For both databases, MSA (measures sampling adequacy) was well below the critical value, thus they are not suitable e.g. for principal component analysis. In both databases, the values of the partial correlation coefficients were much higher than Pearson’s correlation coefficients. Often the signs of partial coefficients did not match the signs of linear correlation coefficients. One of the main reasons for this is that the correlation between the variables is non-linear. Another reason is that control variables have a non-linear effect on a given variable. In such cases, classical methods should be disregarded and expert models better suited to the problem should be chosen in order to analyse the correlation system.
-
Problem analysis of health tourism sector
27-31Views:200On the basis of experts health tourism is one of the most dynamically developing sectors within tourism. The outstanding role of the sector is partly owing to increasing health consciousness and partly to aging society – beside numerous other factors. But health tourism in Hungary is hit by several problems in spite of the fact that recently particular attention is paid to this sector. It can be mentioned as a difficulty that supply of education system is not in conformity with market demands, thus fresh supply of experts is not appropriate. As a world trend, classic position of thermal baths based on natural curative factors is getting to be displaced by adventure baths, which is unfavourable for health tourism. Notwithstanding that Hungary is in a distinguished place on a world scale as regards tourist arrivals, yet we are only in a mid-filed position relating to industrial revenues. Suppliers of this sector do not pay enough attention to disabled persons. The established TDM Organisations have to cope with numerous challenges. Nevertheless experts agree that high support of health tourism can be suggested despite to problems, market risks and significant international competition. However, this support has to be more aimed than before and has to be rather founded on world trends.
-
Application of semen evaluation techniques
5-11Views:646Laboratory methods of semen evaluation are used to select males for artificial insemination. The current review describes several techniques that have been recently used for sperm analysis. Conventional microscopic methods in combination with the objective computerassisted sperm motility and morphology analyzers and flow cytometry, allows to obtain more precise information about the membrane and functional status of spermatozoa. By using several methods to detect motility, viability, acrosomal and capacitation status besides DNA integrity sperm biology and some of the mechanism involved in sperm cry injury can be better understood. The number of possible targets related to sperm quality is increasing, and possible that some of them could enable sperm analysis for predicting freezability and fertility to be improved.
-
Effect of Silver willow “Russian olive” (Elaeagnus angustifolia) on extensive sheep management
157-162Views:221We carried out a study in the Karcag Research Institute, which affected the areas of narrow-leaved silver willow. In the framework of this research, we performed Balázs's coenology, and thus established the degree of Borhidi degradation, which resulted in the fact that the areas of the silver willow were degraded practically irreversibly, the diversity of the grassland has decreased. We consider it very important to study these grassland areas of silver willows, as they can provide an additional fodder base for sheep grazing, which will also increase the sustainability of the grassland. The obtained soil analysis results show that the soil samples of the silver willow areas are richer in nitrogen (p-value: 0.006) and phosphorus (p-value: 0.003) than the examined control area.
-
Analysis of the Relation Between the Relief and the Surface Water Network
90-93Views:139The Bihar plain situated in the Great Hungarian Plain has altitudinal values between 87 and 108 meters above Baltic level and these low average values decreases from East to West. We can find on this place a surface water network with a high density; the most of them was created for diversion of inland water.
The GIS is the best practice for modelling and simulating the relief and the water network. Towards the creation of the TIN model and relief- analysis we need the digital elevation model as well as the digital water network dataset for the whole territory. The source of the data was topographic maps on high scale level (1:10.000). -
Examinations connected with productive life in Hungarian Holstein Friesian populations
32-39Views:368In Hungary, an intensive dairy population has developed in the last few decades as the result of upgrading to Holstein-Friesian. Intensive milk production, adjusted with housing and feeding, unfavorably affected secondary traits. The number of days between two calvings has increased remarkably and productive lifetime has decreased drastically. In the interest of profitable milk production, it is important that cows should be able to stay in the herd as long as possible, in order to have great lifetime productivity.
The aim of this paper was to compare the production and pedigree of cows with eight or more lactations to cows which were culled after their first lactation.
It can be stated that cows with longer herd lives produced more milk in their first lactation than those which were culled after their first lactation. When analyzing the pedigree effect of sires, this could not be proven, although some bulls, mainly of Hungarian origin, appeared at a higher frequency. In the analysis of the effect of dams, it was found that many of the offspring of the cows with longer herd lives had similarly great lifetime productivity. Correlation between herdlife and milk production quantity traits was found to be mild or stronger (r>0,60), while according to our analysis, there is no correlation between herdlife and milk production quality traits. -
Phylogenetic studies of Phoma species by maximum likelihood analysis
37-46Views:395The cosmopolitan Phoma genus contains mainly phytopathogenic, opportunistic parasite, and saprophyte fungal species. Up to now the characterization of Phoma species and other taxa of Phoma has so far been determined on the basis of morphology on standardized media, and gene sequence analysis was only used as a confirmative or distinctive complement.
In this study we have tried to study phylogenetic relationships by maximum likelihood method in the Phoma genus. We employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1) containing both introns and exons, a part of the gene responsible for synthesis of tubulin protein and ITS region containing the internal transcribed spacer regions 1 and 2 and the 5.8S rDNA as potential genetic markers to infer phylogenetic relationships among different Phoma taxa. Twenty-four isolates of eleven different Phoma species were firstly characterised by morphologically, and then their tef1, tubulin and ITS sequences were sequenced and analysed by maximum likelihood method carried out by PAUP*4.0b program. According to constructed phylogenetic trees, the different Phoma taxons are well separated. However these trees do not support the traditional Phoma sections based on morphological characterization.
The maximum likelihood analyses of all three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The experienced molecular evidences initiate the demand of reclassification of formerly mentioned soybean pathogens. -
Definition antioxidant activity of selenium-enriched food sprout, as well as their microbiological analysis
25-30Views:606In this present study, we prepared selenium-enriched food sprouts, where the antioxidant capacity was analysed, we also determined their microbiological status. We took into account the fact, we choose micronutrients to our treatment, that selenium can be delivered to the body by a small amount with the most widely consumed food.
We focused during our research to determine that the increasing concentrations of selenium treatment, in which we used sprouts, knowing fully well that it has an impact on aboriginal antioxidant capacity of sprouts, which is mainly due to high vitamin content of sprouts.
Furthermore, we think it is important to make microbiological analysis, because germination conditions, for example temperature, pH, all this will create an ideal environment for the growth of microorganisms. So we had goal to determine, how the used selenium concentration affect the total plate count, coliform bacteria count and Staphylococcus aureus count of sprouts.
We determined the aboriginal water-soluble and lipid-soluble antioxidant capacity of sprout with the PHOTOCHEM chemiluminometer and we applied pour plate technique for the mapping of the mycrobiological state of sprouts.
Experimental results are evaluated, we state that increasing concentrations of selenite or selenate treatment, is primarily water-soluble antioxidant capacity of sprouts was affected. The water-soluble antioxidant capacity of wheat sprout was much higher than the measured values in pea sprout, this may be linked to what we measured. That is much higher ascorbic acid content in case of wheat sprout, which is well known as one of the most important antioxidant properties compounds of wheat sprout.
We conclude from the results of the microbiological, that the highest concentrations of selenite or selenate treatment has a relative significant anti-microbial effect in case of wheat sprouts. Coliform and total plate count showed no clear decreasing tendency, although the values of treatments in both cases obtained were below the control values.
-
Economic impacts of applying EU animal protection regulations in hog breeding farms
76-80Views:303Hungary’s intention to join the EU makes it necessary to adopt, introduce and use the EU system of law. In Hungary, the legal control of animal welfare has improved (XXVIIIth law in 1998); however, most of our pig farms do not meet the EU animal welfare law requirements for some reason. We examined 9 pig farms in Hajdú-Bihar, Borsod-Abaúj-Zemplén and Heves counties. We chose those farms which use the combined breeding
technology most frequently used in Hungary. The most important part of the welfare directive is the definition of the minimum space per animal. We analysed the data in comparison with EU laws. On the basis of the analysis, it can be said that there is a narrow cross-section: the breeding of piglets. During the cost analysis, we analysed cost and highlighted the permanent cost. We studied how these costs would change if EU animal welfare laws were observed. We also examined the specific data per 1 sow and per 1 kilogram of slaughter pig. We compared the present data (1999) with those we get if EU animal welfare laws concerning minimal space per pig were now followed. It can be stated that after decreasing the sow live-stock in accordance with EU directives, the permanent cost would increase by 17,7% per sow. If the required space per sow were provided, the total cost per sow would increase by 1,9% from 421,1 thousand forints to 429 thousand forints, on average. This would mean a decrease of 7,9
thousand forints profit per sow. As with the decrease of the number of sows, the number of slaughter pigs also decreases. Total cost per 1 kg of slaughter pig would increase from 214,7 forints to 218,2 on average. -
Determination of the validation parameters of inductively coupled plasma mass spectrometer (iCP-mS): response curve linearity in the case of arsenic and selenium
67-71Views:487In the field of elemental analysis inductively coupled plasma mass spectrometers (ICP-MS) have the best sensitivity that means the lowest limit of detection, subsequently their applicability for the detection of essential and toxic elements in foods and foodstuffs is prominent. For the most elements could be measured the detection limit is between μg kg-1 (ppb) and ng kg-1 (ppt) e.g. for arsenic and selenium.
Considering an analytical task (sample type, analytes and their concentration, pretreatment procedure etc.) the applicability of an analytical method is determined by its performance characteristics. The purpose of validation is to ensure that the method would be used fulfills the requirements of the given task. In this article we describes one of the performance characteristics, the linearity, and the whole validation procedure aims measurement of arsenic and selenium in foodstuffs by inductively coupled plasma mass spectrometer (Thermo XSeries I.); but because of the limited number of pages the results are demonstrated only for arsenic.
The linearity of calibration was evaluated in three concentration ranges (0.1–1 μg l-1; 1–10 μg l-1; 10–50 μg l-1), with nine line-fit possibilities (without weighting, weighting with absolute or relative deviation; with or without forcing the curve through blank or origin) and different methods (graphical examination, correlation coefficient, analysis of variance).
The best method to ensure the linearity of correspondence between signal and concentration was the ANOVA test. In view of calibrations it was found that the range of 10–50 μg l-1 could be regarded as linear with four line-fit possibilities, and was non-linear between 0.1–1 μg l-1 and 1–10 μg l-1.
-
ROC analízis alkalmazása
4-7Views:248According to publishings in foreign scientific journals, ROC (Receiver Operating Characteristic) analysis is a widely used method for analysing the diagnostic utility of clinical laboratory tests. In this paper, we explain the basic principles of ROC analysis and produce ROC curves, as well as demonstrate some ROC curves, which represent the results of diagnostic tests.
-
The effect of the plant density for the yield of the maize hybrids
50-61Views:332In order to enchance the yield stability of maize, the effect of plant density on yields was studied on a typical meadow soil in Hajdúböszörmény between 2002-2004. In the plant density experiment, we used the method of Béla Győrffy. The plant densities applied therefore 20 to 100 thousand plants/ha by ten thousand scale. The application of fertilizer rates for the maize hibrids in every year were N: 110 P: 90 K: 120 kg/ha. We used a manual soiling-gun in the experiment. In every year we used plant protection techniques against monocotyledonous and dicotyledonous weeds. The harvest was done by hand. The facts were read by variancie analysis and linear regression analysis. The moisture and the temperatures were extreme in 2002, 2003, 2004. We have to mention defficiery of moisture in 2003 which is shown that the hot days number increased. After evaluating our findings we can conclude that most hybrids showed a significant correlation between increased plant density and the volume of yields. On the basis of the experiments we divided the hybrids into four groups: the first group included the hybrids suitable for increased plant density with a wide range of optimal density values; the second group included hybrids, which did not require high plant density, were capable of good individual performance and tended to grow several ears; the third group included flexible corn types, which grew longer ears in favourable years, thus yielded more; and the fourth group included the hybrids, which were sensitive to increased plant density and which showed a narrow range of optimal density values. Finally, plant density determines the yield; we have to consider optimal plant density intervals as well as optimal plant density, and we also have to place a high emphasis on the use of hybrid-specific technologies.
-
Comparative analysis of Carpathian Braunvieh’s morphological traits
15-19Views:611The Carpathian Braunvieh cattle established by the cross-breeding of the Schweizer Braunvieh and the local breeds of the Carpathian basin approximately 150 years ago. The evolved three usage breed was durable and resistant, however in comparison with the high-productivity breeds was less competitive. The dramatic lay-off, and the endangered status of the breed requires a conservation programme. University of Debrecen took the investigation of the Hungarian population and it’s detailed genetic studies. Within this research project among others we carry out body measurements and rates of the body traits. Depending on these results, we may decide on the subsequent gene-reserving objects. Based on body measurements and live weight we established that the breed at Mikóháza favours to the one lived in Hungary in the 1960’s, and to another, named Ukrainian Carpathian Brown. Compared to the earlier data (Horváth, 1966) smaller body traits can be seen. Our livestock is proportionately smaller than the other breeds in height at withers, as well as in live weight. Based on the comparative analysis, we established, that – thanks to the aware sorting – the imported individuals represent the ancient, primitive Carpathian variant.
-
Development Level Analysis of the Settlements and Micro Regions of the Hungarian-Ukrainian Borderland
73-78Views:208This paper aims to make a settlement and micro region-level analysis in case of one of Hungary’s most backwater regions, the Hungarian-Ukrainian borderland. After a brief methodological and theoretical review, it creates a complex development indicator with the help of certain ratios belonging to appropriate groups. It analyzes the exceptionally heterogeneous settlement-level values with applied statistics methods and tries to determine the role of population, population density, the distance from the nearest border-crossing point and the travel time from the centre of the micro region and the county. As its main findings the followings can be mentioned: there are significant development level differences in case of the „periphery of the periphery” micro regions which depend on the number of population, population density and the travel time from the centre of the micro regions.
-
HPLC-MS analysis of the active ingredients of chamomile (Matricaria chamomilla) bee feeding syrup
61-65Views:658Apiarists let prepare by bee honey products containing medicinal drugs. Our aim was to prove that the active ingredients originated from the herbs are also present in the bee products. This publication focuses only to the chamomile (Matricaria chamomilla) containing feeding syrup and the difference between the various syrup phases. To fulfil this task, we developed a method analysing the non-volatile components of the syrup using the flowers of chamomile. The method involves a filtration followed by HPLC-MS analysis. The analyses and the data evaluations proved that the non-volatile components of the herb were transferred to syrup. As the characteristic components of certain herbs could be identified also in the syrup, it is obvious that the components responsible for the medicinal effects are also transferred.
-
Decreasing Energy and Water Use in the Meat Industry
24-28Views:172The technology supervisions are needed from time to time in order to involve the new research results, experience, new technologies and equipments organising principles into the production and to make it cheaper and with less expenditure to increase the profitability and competitiveness. In the course of our investigation we aimed the analysis of the traditional technologies and improving of it. In this article we detail the heat treatment curing process. We applied the principles of experimental design for the determination the main influencing parameters in the processes. Then we made simulations and modelling in seeking for the environmentally better technology programs. We developed a method for measuring the temperature development of products with small diameter using the similarity theory in unit operation. In the course of the analysis of the heat treatment processes we could state that the heat treatment time can be reduced significantly by decreasing the size of the product and choosing the right ratios among the surface heat transfer intensity, ambient temperature and involving the lethality obtained during the cooling phase. The total sparing were about 10-20%. We solved the problem associated with the sensor placement error using a plastic material (metamid). We establish a curve between the real product temperature and plastic rod temperature by which the monitoring of the process became more accurate. We determine the size modification for different product sizes. In case of curing we observed very similar phenomena. For the determination of the diffusion coefficient we adopted the Ball-method not using in this field till today. We could reduce the excess saltiness and loss of NaCl with about 5%.
-
Effects of production factors on the yield and yield component of winter wheat
26-31Views:219The effect of major production factors (forecrop, fertilisation, irrigation, soil cultivation and soil preparation) on the yield components and yield of winter wheat were studied in a long-term experiment set up at the Látókép Experimental Nursery of the Agricultural Sciences Centre of the University of Debrecen. The results of regression analysis led to the following conclusions:
• In our experiments in 2000, after using maize as a forecrop –based on the results of analysis of regression – fertilisation determined the yield.
• After using pea as a forecrop, a N50 P35 K40 kg/ha fertiliser rate led to an economical increase in the yield of winter wheat.
• None of the determinative yield components varied significantly for winter wheat produced after using pea as a forecrop.
• There is a closed, significant correlation between plant height, spike length, plant and spike mass, the number of spikelets and grains per spike after using maize as forecrop. The thousand grain mass is different from the other yield components, because it is not part of the relation system of
those yield components.
• The increased yield of winter wheat after maize has been used as a forecrop is due to the positive change in grain number per spike yield component. -
Analysis of different fertilization settings’ effect in the case of the summer savory’s (Satureja hortensis L.) yield and active agents
101-105Views:310During our research we investigated the Mediterranean summer savory’s (Satureja hortensis L.) nutrient requirement with different fertilization settings in a small plot trial. We measured the medicinal plant’s raw and dry drug mass, and the presence of the main essential oil active agents under the different fertilization settings using SPME (Solid phase microextraction) and GC-MS (Gas Chromatograph mass spectrometer).From the drug’s raw and dry mass perspective, based on the obtained results, in our opinion, the N30P40K60 nutrient requirements were ideal in 2015, while the N60P80K120 nutrient requirement showed the best values in 2016. A significant relationship is assumed between the different fertilization settings and the essential oil active agents’ distribution. During the analysis of data, we compared three of the savory’s essential oil active agents’ percentage of presence in the nutrient requirement samples.