Search
Search Results
-
Impact of nitrogen and sulphur fertilization on the growth and micronutrient content of spring wheat (Triticum aestivum L.)
211-219Views:166Micronutrients are as important as macronutrients for crops. Each micronutrient has its own function in plant growth. Zinc is important for membrane integrity and phytochrome activities. Copper is an essential micronutrient required for the growth of wheat. Manganese is required for enzyme activation, in electron transport, and in disease resistance. The pot experiment was set up in greenhouse on calcareous chernozem soil Debrecen-Látókép with a spring wheat. In certain development stages (according to BBCH growth scale of wheat), at the beginning of stem elongation (29–30), at the heading (51–59), at the flowering (61–69) stage three average plants were removed from all pots for analysis. Fresh and dry weight of the plant samples were measured. Plant leaves after drying were digested by HNO3-H2O2 methods and manganese, zinc and copper contents of plant were quantified by atomic absorption spectrophotometry. At the flowering stage, when the nutrient uptake of plants is the most intensive, the weight of wheat ranged between 0.94–1.57 g plant-1. In this development stage, the NS2 treatment produced the highest weight of wheat, and compared to this the NS3 treatment decreased that value already. The results show unfavourable effect of NS3 treatment. On the basis of microelement content of wheat and the weight of a plant, nutrient uptake by plant were calculated. At the beginning of growth the starter treatment had positive effect on Cu-uptake compared to the NS1 treatment, where the same dose of fertilizer was stirred into the soil. Wheat is very sensitive to copper deficiency, so copper dissolved by starter treatment could be favourable to the early development of wheat. At flowering stage the Zn-uptake of wheat became the highest and it was between 133.7–234.6 mg plant-1. The Mn-uptake of wheat plant was higher than the Cu- and Zn-uptake of wheat.
This phenomenon can be explained by the fact that the untreated soil had higher Mn-content, than Cu- and Zn-content. To summarize the results, it can be stated, that the copper uptake of wheat was more affected by the different treatments in the stage of stem elongation, while Mn- and Zn-uptake of wheat were influenced primarily in the stage of heading and flowering.
-
The importance of millet production in regional production, with special emphasis on climate change
141-146Views:137Regional production is a traditional production structure developed adjusting to the geographical, climatic, biological and soil conditions in given production regions, a certain territorial specification of agricultural production, and a type of farming that best fits the natural conditions and takes the biological needs of plant and animal species into account as fully as possible. The most probable element of risk in plant production is the changeable, extreme weather. That is the reason why the specific characteristics of the place of production and the characteristics of regional production should be considered to a greater extent. The establishment of the range of varieties appropriate for the place of production is the key issue in regional production. One of our historically grown cereal plants that perfectly fits regional production is millet. Due to its short growing season, favourable reproduction ratio and the fact that it is relatively undemanding, it used to be grown in larger quantities in the middle ages. Its good nutritional values made it an important food item, but over time, as a result of industrialisation and technological progress; it has been eclipsed by other cereal crops. In our country it is mainly used to cook porridge, but it is also used in the form of flour and as a base material in the spirit drinks sector. In the recent decades, millet has been applied only in a small area, mostly as a secondary crop in areas that dried out from drainage water in late spring, or as a replacement of extinct sowings due to its late sowing time. Water will be the most significant factor for the future of agriculture, especially considering climate change.My examinations took place in the area of the Institutes for Agricultural Research and Educational Farm of University of Debrecen, in the Research Institute of Nyíregyháza, in a small-plot experiment with four replications in 2016. -
Development of a New Maize (Zea mays L.) Breeding Program
25-30Views:91Genetic manipulation may not replace any conventional method in crop breeding programs, but it can be an important adjunct to them. Plant regeneration via tissue culture is becoming increasingly more common in monocots such as corn (Zea mays L.). In vitro culturability and regeneration ability of corn decreased as homozigosity increased, which suggested that these two attributes were controlled primarily by dominant gene action. Pollen (gametophytic) selection for resistance to aflatoxin in corn can greatly facilitate recurrent selection and screening of germplasm for resistance at a much less cost and shorter time than field testing. Integration of in vivo and in vitro techniques in maize breeding program has been developed to obtain desirable agronomic attributes, speed up the breeding process and enhance the genes responsible for them. The efficiency of anther and tissue cultures in most cereals such as maize and wheat have reached the stage where it can be used in breeding programs to some extent and many new cultivars produced by genetic manipulation have now reached the market.
-
The influence of fertilization and tillage method on the formation root system capacity and grain production of spring barley
89-93Views:124To evaluate the interaction of year x variety, year x tillage method and year x fertilization on the grain yield and root system capacity (RSC) of spring barley, we ran polyfactorial field trials in agroecological conditions of a warm corn production area in Slovakia, at Malanta, in 2009 and 2010. The RSC measurements were done using LCR - meter at a frequency of 1 kHz and they took place in four growth stages: at leaf development in the stage of four leaves (RSC1), in full tillering (RSC2), in the stage heading (RSC 3) and at the stage of ripening (RSC4). The values of grain yield, RSC1, RSC2, RSC3, RSC4 reached in 2009 comparison to 2010 were significantly lower. The highest yield in 2009 was reached by variety Marthe (4.49 t.ha-1) and by variety Bojos (7.19 t ha-1) in 2010. The highest values of RSC in observed growth stages were achieved by variety Bojos in 2009, and in 2010 also besides RSC1. Within both years, difference in yields between tillage methods was not observed. The values of RSC in growth stage of 4 leaves and tillering was higher at conventional tillage, butthe values of RSC3 and RSC4 were higher with minimized tillage. The highest grain yield and values of RSC in every growth stage were achieved on the fertilization variant “c“ in 2009 and on the fertilization variant “b“ in 2010. The correlation relationships between grain yield and RSC were significant and positive in every growth stage. The strongest relationship was found among grain yield and RSC (r=0.6047).
-
Foreword
9-10Views:141The higher education of economics and agriculture in Debrecen started 150 years ago. The historical roots of our institution reach back to the middle of the 19th century. In the 1850s, the wise leaders of the city of Debrecen realised that the development of agriculture is the sole opportunity which can make the region of the Tisza River and the Trans- Tisza region the most valuable pantry of the nation. Due to the generous support of the citizens of Debrecen, as well as the enthusiastic work of scientist and teachers, the National Higher Education Institute of Farming was established on 22nd October 1868 as the first agricultural higher education institution of the Great Hungarian Plain.
-
Leaf reflectance characteristics and yield of spring oat varieties as influenced by varietal divergences and nutritional supply
29-34Views:90Inadequacy of nutrients in the soil and sufficient inaccessibility to nutrients is caused by factors that affect production and productivity of spring oat varieties. Exogenous application of nutrient and real time nutrient assessment can therefore reverse these associated negative consequences. A field experiment was conducted to evaluate the response of spring oat varieties to sulphur and silicon based fertilisation. Eight spring oat varieties, four level of nutrient application was arranged in a split-plot design with three replications. The obtained results showed that foliar application of sulphur improves the grain yield of most nutrients responsive varieties by about 34.7%. However, application of silicon had shown a diminishing return association to grain yield of variety GK Kormorán, GK Pillangó, Lota, Panni. LAI, thousands grain weight, SPAD, NDVI was significantly (p < 0.05) influenced by genetic difference of the tested varieties, developmental plasticity, and nutrient application. Significantly higher grain yield was obtained from the variety Mv Pehely than the other tested varieties. Therefore, it could be inferred that a combined use of nutrient responsive spring oat varieties and sulphur containing fertilisers could be important agronomic practice to improve grain yield and to develop climate resilient oat varieties.
-
Evaluation of water balance in apple and pear trees
193-198Views:103A significant proportion of the aboveground green and dry weight of the plant constitutes the foliage. The canopy is an important factor
of plant growth. On one hand the canopy absorbs the solar energy, which is necessary for the photosynthesis, on the other hand accumulates
the absorbed nutrients by the roots, and the most of the water-loss happens through the foliages. The determination of the full canopy is not
an easy target. In our research we developed a measurement method to determine the leaf area. With the parameters of the examined tree
(leaf length and maximum width) and the data of ADC AM 100 leaf area scanner we determined the k-value, with which we can easily and
fast evaluate the leaf surface. Furthermore we defined from the water balance of compensation lysimeters the cumulative transpiration of
fruit trees and the efficiency of water use of trees. -
Folic acid content of beetroot leaf and root by different growing stages and genotypes
115-119Views:227An increasing interest has been observed of beetroot leaf as a salad component due to recent studies focusing on their nutritional value. The randomized field experiment was carried out on lowland chernozem soil with 6 varieties, 3 replications and 2 sowing dates. Sampling was performed on 23 of August 2018 at the stage of 30 and 50 days of vegetation, where leaf (30 and 50 days) and root (50 days) were collected. Total dry matter, folic acid and nitrate content were evaluated.
The results of this investigation show that higher total dry matter content was measured in the root (8.47–10.30%) compared to the leaf in both developmental stages (6.47–9.20%). Nevertheless, higher folic acid content was found in the young leaves of 30 and 50 days of development (58.77–113.86 µg 100g-1). Among the examined varieties, Bonel has presented great amount of folic acid not only in the leaves (99.35–113.61 µg 100g-1), but also in the root (89.99 µg 100g-1). Finally, lower nitrate content was found in Libero (316.16 mg kg-1) at 30 days and in Akela (340.41 mg kg-1) at 50 days of development. Thereby, fresh consumption of beetroot leaves are highly recommended.
-
Price Risk Management by Futures Markets and Public Warehousing
58-65Views:82The principal achievement of this paper is the combinative use of two market institutions: public warehousing and commodity exchange and how their joint application is beneficial for the players on the grain market. Based on a theoretical foundation, a calculation model was developed in order to assist short and long-term marketing decisions. It allows all the three participants of the market: producers, consumers and traders, to use this model in order to establish their own business strategy. The model can be used to analyse factors influencing the establishment of price; therefore, it can be also used for policy-making decisions.
-
Growth and clorophyll content dynamics of winter wheat (Triticum aestivum L.) in different cropyear
101-105Views:107The experiments were carried out at the Látókép experimental station of the University of Debrecen on chernozem soil in a long term winter wheat experiment in the season of 2011 and 2012 in triculture (pea-wheat-maize) and biculture (wheat-maize) at three fertilisation levels (control, N50+P35K40, N150+P105K120). Two different cropyears were compared (2011 and 2012).
The research focused on the effects of forecrop and fertilisation on the Leaf Area Index, SPAD values and the amount of yield in two different cropyears. We wanted to find out how the examined parameters were affected by the cropyear and what the relationship was between these two parameters and the changes of the amount of yield.
Examining the effects of growing doses of fertilizers applied, results showed that yields increased significantly in both rotations until the N150+PK level in 2011 and 2012. By comparing the two years, results show that in 2011 there was a greater difference in yields between the rotations (7742 kg ha-1 at N150+PK in the biculture and 9830 kg ha-1 at N150+PK in the triculture). Though wheat yields following peas were greater in 2012, results equalized later on at N150+PK levels (8109–8203 kg ha-1).
Due to the favorable agrotechnical factors, the leaf and the effects of the treatments grown to a great extent in 2011, while in 2012 the differences between treatments were moderate. Until the N150+PK level, nitrogen fertilisation had a notable effect on the maximum amount of SPAD values (59.1 in the case of the biculture and 54.0 in the triculture). The highest SPAD values were measured at the end of May (during the time of flowering and grain filling) in the biculture. In the triculture, showed high SPAD values from the beginning. The same tendency could be observed in the 2012 cropyear, although increasing doses of fertilizers resulted in higher SPAD values until N150+PK level only from the second measurement. Maximum SPAD values were reached at the end of May in both crop rotation system
-
Data on the bumblebee assemblages (Apidae: Bombus spp.) lives in lands under agri-environment commitment
31-35Views:199The goal of agri-environmental schemes (AES) and greening programs are protecting and increasing biodiversity in agricultural lands. The evaluation of effectiveness of AES needs further investigations. For the purpose of investigations, species and species groups should be selected which can indicate the effects of changes in landscape use on biodiversity. Bumblebees are good indicators for this purpose.
The role of bumblebees in pollination is well studied but in the case of different crops, much less detailed data are available. In 2018, bumblebee assemblages of 44 sites belonged to 8 different agricultural and semi-natural habitat types were studied in the surroundings of Sajószöged, Tiszaújváros and Derecske.
This study provides new distribution data of 8 bumblebee species in three 10×10 km UTM cells covering the sampling area. According to our results, the alfalfa and red clover fields and semi-natural grasslands has more species rich and abundant bumblebee assemblages than different crop fields (sunflower, oilseed radish and vegetable morrow) and can help protect bumblebee assemblages of agricultural lands. Based on the collected distribution and abundance data, the role of the bumblebees in pollination of the studied crops should be re-evaluated.
-
Marginalisation and Multifunctional Land Use in Hungary
50-61Views:75Our study prepared as a brief version of National Report in the frame of EUROLAN Programme. We deal with the interpretation of some definitions (marginalisation of land use, multifunctionality of land use, marginalisation of agriculture, multifunctionality of agriculture), with sorting and reviewing indicators of marginalisation and finally with the analysis of functions of land use. We suggested a dynamic and a static approach of marginalisation. We can explore the dynamic process by time series and the static (regional) one by cross-section analyses.
It is very hard to explain the perspective of the future of marginalisation of land and of agriculture in Hungary. The process of marginalisation seems faster in the agriculture in the coming years, but it depends on the utilisation of new possibilities given by the EU financial resources and by the Common Market. At this moment agriculture seems one of the big losers of the accession.
In the long term we should face considerable challenges in the land use. It is necessary to take into account that there is a supply market of foods and traditional fibre production world-wide. There are limited possibilities to produce and to market for example biodiesel (fuel), bioethanol, or maybe biogas. Thus the environment and landscape preservation becomes more and more real land use alternatives.
The environmental interpretation of the multifunctionality of land use: activities (functions) of environmental preservation and nature conservation in a certain area, which aim to preserve natural resources by the existing socio-economic conditions.
Preservation of rural landscapes is the task mainly for land-users, who can be commanded by legal means and can be encouraged by economic measures to carry out the above activity. In the recent past measures of „command and control” type regulation were predominant, however nowadays, especially in the developed countries, the role of economic incentives increases.
As a conclusion of our analysis we can state that as long as the main land-dependent activities (agriculture, forestry, housing, tourism, local mining) cease to be viable under an existing socio-economic structure, then it is hardly possible to sustain the rural landscape on an appropriate level by non-commodity products (such as environment preservation, cultural heritage, nature conservation, employment etc.).
1 The study was prepared in the frame of EUROLAN (EU-5 Framework Project), QLK5-CT-2002-02346, as a compiled version of the Hungarian National Report, The national project co-ordinator: Prof. Dr. Gabor Szabo.
A part of places with high ecological values coincides with the areas with unfavourable agricultural endowments and underdeveloped micro-regions. We think so that the marginalisation preserves the non-environmental-sound activities and hinders the development of multifunctional agriculture and this process can change only by joint utilisation of endogenous and exogenous resources and methods. Thus the successful programmes for agri-environmental protection and multifunctional land use can serve the moderation of negative effects of marginalisation or maybe the marginalisation process itself. -
The main influencing factors effecting the yield of maize
137-141Views:88Maize is one of Hungary’s major cereals. In the 1970s and 1980s, we were in the frontline regarding yields and genetic advancement. However, yield fluctuation in maize has increased to 50-60% from 10-20% since the 1980s, which was partly caused by the increase in weather extremes due to climate change and by agrotechnical shortcomings.
The experiments were carried out on typical meadow soil in four repetitions in the period of 2007-2008. In the sowing time experiment, sowing was performed on 10 April, 25 April, 15 May under a uniform fertilization of N120, P2O580 K2O 110 kg/ha. In the fertilization experiment, the yielding capacity of 10 hybrids with different genetic characteristics was studied in a control (non-fertilized) treatment and basic treatment of N40 P2O5 25, K2O 30 kg ha-1 active ingredient and a treatment with fivefold dosages of the basic treatment. In the plant density experiment, the relationship between plant density and yield was analysed at plant densities of 45, 60 and 75 thousand plants per ha. We found a tight correlation between sowing time and yield and grain moisture content at harvest. We found that grain moisture can be reduced by 5-10% by applying an earlier sowing time.
The agroecological optimum fertilizer dosage was N 40-120, P2O5 25-75, K2O 30-90 kg ha-1 active ingredient at a plant density of 60-90 thousand plants ha-1 depending on the hybrid and the year. -
Long term researches regarding the irrigation influence on sugarbeet crop inthe Crisurilor Plain
11-15Views:111The paper is based on the researches carried out in the long term trial placed on the preluvosoil from Agricultural Research and Development Station Oradea, Crisurilor Plain in during 1976–2012.
The soil water reserve in 0–75 cm depth decreased bellow easily available water content every year and in 32% of years the soil water reserve decreased bellow wilting point. For optimum water supply an irrigation rate of 2665 m3 ha-1 (variation interval 500–5090 m3 ha-1) was needed.
The irrigation determined improving of water/temperature + light report (Domuta climate index) with 47.4% in average in the period May–September. A statistically very significant connection was quantified between this indicator and the yield.
Daily water consumption increased in the irrigated variant, the biggest difference in comparison with unirrigated variant was registered in August, 86% in comparison with unirrigated variant. As consequence, the value of the total water consumption increased with 50%, variation interval was 11–154%. The irrigation covered 37.8% of total water consumption, the variation interval was 8.3%–67.9%.
The yield level of the sugarbeet increased in average with 61%, the variation interval was 9–227%. Standard deviation was lower in the irrigated variant and this emphasizes an improve of the yield stability with 25.1%. The sugar content of the sugarbeet roots from irrigated variant increased statistically very significantly in the droughty years and differs significantly in the rainy years.
Water use efficiency increased in the irrigated variant with 7% and irrigation water use efficiency was between 7.9 kg yield gain 1 m-3 irrigation water and 17.4 kg yield gain 1 m-3 irrigation water.
The positive influence of the irrigation on microclimate, water consumption, yield level, stability and quality and on water use efficiency sustain the need of the irrigation in sugarbeet from Crisurilor Plain.
-
Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)
93-96Views:147Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered next to Debrecen city (Hajdú-Bihar county, East Hungary).
In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.
The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.
-
Labour productivity in the Hungarian agriculture
119-128Views:149This paper presents the situation of the agricultural labour employment and evaluates the agricultural labour productivity in Hungary. Since Hungary’s EU accession, the share of agricultural employment in the total employed population has been stabilized at around 5% in the country. Due to low wages, low profit and low prestige, agricultural jobs are not attractive. The gross and net labour productivity gap between Hungary and the EU have been significant since the EU accession. The agricultural labour productivity of the Hungarian regions also shows a different picture. The labour productivity influences the cost, profitability and competitiveness of products. The advantage of the cheap labour force in Hungary’s agriculture significantly decreased due to low labour productivity. There are several opportunities to increase the agricultural labour productivity such as the increase of labour force qualification, moving toward producing higher value added agri-food products, rejuvenation of population in agriculture and the improvement of the conditions of financing agriculture.
-
Competence modules of the agro-environmental engineering bachelor course in Hungary
229-236Views:69One of the most important goals of the Bologna Process is to introduce the three circle system: bachelor, master, doctorate courses. In Hungary from 2006/2007 academic year 132 bachelor courses are launched. One of the launched bachelor course is environmental engineering of which the scientific background and economical sector are rapidly changing. Hence, to help graduates from this area to find a job, it is very important to examine working activities, expectations of employers, to study requirements of the training programs, the system of competencies. Having examined these points, frameworks of professional requirements have been worked out by 27 validation tests. In this publication, the results of the research work related to the environmental engineering bachelor program were presented.
-
The effect of crop protection and agrotechnical factors on sunflower in the Hajdúság region
39-46Views:79Extreme weather conditions are becoming more and more frequent in the crop years, thus increase the risk of sunflower production.
The objective of researches into plant production is to minimize these effects as much as possible. In this sense, the optimization of
agrotechnological factors is of high importance. Within these factors, the appropriate crop technology (sowing time, crop density)
and optimized, rational crop protection technologies are important, especially in the highly sensitive sunflower cultures. The effect of
sowing time, crop density, and fungicide treatments on the yield of sunflower hybrids was analysed in different crop years in 2008
and 2009. In each case, the infection was highest with the early sowing time and at the highest crop density level (65000 ha-1). When
one fungicide treatment was applied, the rate of infection decreased compared to the control treatment. The further decrease of the
infection rate was less after the second fungicide treatment.
In the humid year of 2008 the crop yield was the highest at 45000 ha-1 crop density level in the control treatment and at 55000 crop
ha-1 crop density level when fungicides were applied. In the draughty year of 2009 the maximum yield was gained at 55000 ha-1 crop
density level in the control treatment and at 65000 crop ha-1 when fungicides were applied. In 2008 and 2009 as regards the crop
yield, the difference between the optimal and minimal crop density levels was higher in the fungicide treatments than in the control
treatment (in 2008: control: 517 kg ha-1; one application of fungicides: 865 kg ha-1; two applications of fungicides: 842 kg ha-1), (in
2009: control: 577 kg ha-1; one application of fungicides: 761 kg ha-1; two applications of fungicides: 905 kg ha-1).
In each and every case, the first treatment with fungicides was more effective than the second. In 2008, the highest yield was
obtained with the third, late sowing time in each fungicide treatment. The differences between the crop yields with different sowing
times was less than in 2009, when the results of the second treatment exceeded those of the first and third treatment in each case. -
Nitrogen Supplying Capacity of Brown Forest Soil under Different Cropping Practices and 0.01 M CaCl2 Soluble Organic Nitrogen
17-23Views:86The best known and most remarkable example of continuous production in Hungary is the Westsik’s crop rotation experiment, which was established in 1929, and is still in use to study the effects of organic manure treatment, to develop models, and predict the likely effects of different cropping systems on soil properties and crop yields. In this respect, Westsik’s crop rotation experiment provides data of immediate value to farmers concerning the applications of green, straw and farmyard manure, as well as data sets for scientific research.
Although commonly ignored, the release of nitrogen by root and green manure crops has a significant impact on soil organic matter turnover. The design of sustainable nitrogen management strategies requires a better understanding of the processes influencing nitrogen supplying capacity, as the effects of soil organic matter on soil productivity and crop yield are still very uncertain and require further research. In the treatments of Westsik’s crop rotation experiment, nutrients removed from soil through plant growth and harvesting are replaced either by fertilisers and/or organic manure. Data can be used to study the nitrogen supplying capacity of soil under different cropping systems and its effect on the 0.01 M CaCl2 soluble organic nitrogen content of soil.
The aim of this paper is to present data on the nitrogen supplying capacity of brown forest soil from Westsik’s crop rotation experiment and to study its correlation with hundredth molar calcium-chloride soluble organic nitrogen. The main objective is to determine the effects of root and green manure crops on the nitrogen supplying capacity of soil under different cropping systems. The nitrogen supplying capacity was calculated as a difference of plant uptake, organic manure and fertiliser supply.
The 0.01 M CaCl2 soluble organic nitrogen test has proved reliable for determining the nitrogen supplying capacity of soils. Brown forest soils are low in organic matter and in the F-1 fallow-rye-potato rotation, the nitrogen supplying capacity was 15.6 kg/ha/year. 0.01 M CaCl2 soluble organic nitrogen content was as low as 1.73 mg/kg soil. Roots and green manure increased the nitrogen supplying capacity of soil by more than 100%. This increase is caused by lupine, a legumes crop, which is very well adapted to the acidic soil conditions of the Nyírség region, and cultivated as a green or root manure crop to increase soil fertility. -
Adopt the tobacco plastid transformation technics in Hungary
73-76Views:69Under the GENOMNANOTECH Debrecen Regionális Egyetemi Tudásközpont (GND RET) research program in summer 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture experiments were launched to transform tobacco plant by plastid transformation technics.
Our aim was to adopt first in Hungary the tobacco plastid transformation technics, which were used in Waksman Istitute Rutgers, The state University of New Jersey (USA) leading with Prof Dr. Pál Maliga. Scientists won scholarships learn and use this technics in the University of Debrecen. -
Effect of G2548A polymorphism in the leptin gene on the BMI level in human population
5-10Views:129The polymorphism in leptin (LEP 2548A) seems to influence obesity among others genes. The aim of this study is to investigate the effect of the G2548A polymorphism on body mass index. We included 79 people from Slovakia with some genetic relatedness and used barrels kit to isolate the genomic DNA from an adenoblast swab- from the salivary. PCR products were amplified by pursued polymorphisms and G2548A, we restriction-analyzed them and then we identified the specific fragments describing the presence of chosen SNP polymorphism by the agarose electrophoresis, to analyze SNP polymorphism by PCR-RFLP method.
The LEP gene had increased frequency of G allele (0.5506). The most common genotype occurring in the gene LEP was heterozygous genotype (AG) and the least frequent genotype in LEP was AA (0.1899). Taking the age into account the BMI is higher if the G allele occurs in the LEP gene. Moreover, if the G allele genotype was situated in dominant form, then the highest average BMI was present.
According to the results we can assume that the AA genotype (LEP) has a protective effect on the prevalence of obesity compared to the other genotypes.
-
Leaf and root evaluation of bioactive compounds of different beetroot varieties
135-139Views:196The importance of beetroot lies in the numerous health benefits, attributed to its wide-ranging array of minerals, vitamins and bioactive compounds which play a key role in the human diet. Generally, beetroot is consumed for its root, however, its leaves also contain a good amount of bioactive compounds. The experiment was carried out with four beetroot varieties in order to compare the amount of bioactive compounds in the root and the leaf. The analyzed data were total dry matter content, total polyphenol, flavonoid and vitamin C.Results have shown that roots contain the highest amount of bioactive compounds. Nevertheless, leaves can be also considered a significant source. Therefore, consumption of the young leaves is highly recommended due to their availability after thinning or at maturity stage (60 days after the germination). Of the examined varieties, ‘Libero’ demonstrated the highest total polyphenol, flavonoid and vitamin C values, although it was found to be statistically insignificant. -
Nutrient Uptake of Miscanthus in vitro Cultures
23-24Views:72The large biomass production and the low necessary input fertilizer make Miscanthus an interesting, potential non-food crop with broad applications, e.g. for fuel and energy, for thatching, fiber production, for the paper and car industries, as well as for ethanol production.
Axillary buds of Miscanthus x giganteus were placed on a shoot inducing nutrient solution (modified Murashige and Skoog, 1962), basic medium supplemented with 0,3 mg l-1 6-Benzylaminopurin. After 40 days of culturing, the axillary buds produced three times more shoots than could normally be harvested. The nutrient content (N, P, K, Ca, Mg) was measured several times during culturing. The results showed that, after 35 days, nitrogen and phosphate were nearly completely taken up. From that time, shoot growth was not observed.
After shoot propagation, the plants were transfered into a nutrient solution for root formation (modified Murashige and Skoog, 1962), basic medium supplemented with 0,5 mg l-1 Indole- 3-Butyric acid, and could be potted in soil after about 14 days. -
Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:273The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.
-
Impact of weather on the spring crops yield in Croatia with emphasis on climatic change and the 2014 growing season
41-46Views:157Main field crops in Croatia are maize, soybean, sunflower and sugar beet. By these crops are covered (status 2014) close to 50% (385 234 ha) of utilized arable land. Global warming, have often adverse influence on field crop yields. Aim of this study was testing precipitation and temperature regimes on spring crops yield in Croatia in 15-year period (1999–2013) and elaboration of the 2014 growing season with aspect of climatic change.
Four growing seasons (2000, 2003, 2007 and 2012) were less favorable for maize because annual yield was bellow 5 t ha-1 (average 4.38 t ha-1), while in four more favorable years (2005, 2008, 2009 and 2010) annual yield was above 6.8 t ha-1 (average 7.32 t ha-1). Average precipitation and temperature for the April-September period in Osijek were 226 mm and 496 mm, 19.6 oC and 18.6 oC, for less and more favorable years, respectively. Yields of soybeans and sugar beet have mainly similar trend as maize yields in function of weather conditions, while sunflower is more susceptible to extremely moist growing seasons (for example, 2001 and 2005: 650 mm and 697 mm precipitation and very low yields in level 1.7 and 1.6 t ha-1, respectively). On the other side, under drought conditions of 2003, 2007 and 2012, yields of sunflower were above average in range from 2.5 to 2.7 t ha-1), while at same period yields of maize, soybean and sugar beet were drastically reduced.
Average precipitation in the April-September period of 2014 for eight selected sites of Croatia was 756 mm or for 68% higher in comparison with the long-term average 1961–1990 with variation among the sites from 520 mm in Osijek to 910 mm in Varazdin. On the other side, average air-temperature in 2014 was 17.8 oC or for 0.7 oC higher with variations among the sites from 17.2 oC in Daruvar and Varazdin to 18.2 oC in Osijek and 18.3 oC in Gradiste. Under these favorable weather conditions, annual yields of maize (8.1 t ha-1), soybeans (2.8 t ha-1) , sunflower (2.9 t ha-1) and sugar beet 63.6 t ha-1) were considerable higher than usual.