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Leaf protein analyses in order to utilise the leaf shoot of artichoke
43-47Views:129The constat growth of the Earth’s population brings with itself a higher demand for food and protein not only in human nutrition but also for the feeding of livestock. Currently, the feed industry is mainly built onseed-based protein, wherethebaseplant is soybean, which is large lycovered by imports in Hungary, similar toother European countries. However, the long-term economically sustainable lifestock breeding demandschanges which has also worked out strategies. An alternative protein sources could be green leafy plants.
In current work the Jerusalem artichokes as an alternative source of protein was studied, compared to alfalfa as a valuable protein plant. Our results indicate that fiber fraction ofJerusalem artichoke shootswas 34 to 37% after pressing in the autumn period while alfalfa slightly lower values were obtained (30%). On the other hand extracted green leaf protein concentration was higher in alfalfa than in Jerusalem artichoke. Along with this higher protein content could be measured from the leaf protein concentration of alfalfa and almost each amino acids were more, as well comparing to Jerusalem artichoke.
Overall, the alfalfa proved to be advantageous as expected both in leaf protein extraction efficiency, both regarding the content of the protein in the Jerusalem artichoke. However, considering aminoacid composition and green biomass production, Jerusalem artichokecould be a promisingplant species asplant protein sourceinthefuture.
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Comparative research of the Wild Sage (Salvia nemorosa L.) population and selected clones flowering biology
113-118Views:125The collected and evaluated of morphological Salvia variants widespread application provides for a new type of hungaricum series create. The excellent drought and frost tolerant Salvia nemorosa L. is climate change-tolerant perennials include. At the right time carried out with the new propagation cutting method can be shortened the seedling time, because in 1 year from broke cuttings develops flowering population. The flowering period of Salvia during one vegetation period may extended with right fertilization and phytotechnical, so it becomes suitable for planting in public spaces.
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Seed biology and possibility of improvement of seed germination capacity on Virginia mallow (Sida hermaphrodita L. Rusby)
71-76Views:136Sida hermephrodita or Virginia mallow is a perspective perennial herb in the Malvaceae family able to yield a biomass crop through the last two decades. Additionally, the plants have a lot of uses and benefits for instance it can use as a fodder crop, honey crop, ornamental plant in public gardens. It has favourable features like fast growing and resistance against the disease and climatic fluctuations, etc. Sida is in the beginning phase of domestication therefore it has a serious disadvantage: the low and slow germination as a big part of wild plants. Due to the expressly low germination percent the need of seed showing of driller is should tenfold, 200 thousand seeds/acre instead of 10–20 thousand what is not available and expensive Therefore practical purposes of our research of seed physiology was to increase the seed germination percent in a available, basically wild Sida population. In the first stage of our experiments we examined two factors relating to seed germination percent and seed germination power during our research: the influence of hot water treatment and the effect of exogenous or endogenous infection of seed. However, in our germination tests, utilizing scarified seeds with hot water (65 oC, 80 oC, 90 oC), from 29,3% to 46% germinated from those samples which were collected from the population of Sida hermaphrodita in Debrecen. The average germination for all season was 5–10% without treatment and rinsed using hot water up to almost 50%. When physically scarified used, the oldest seeds showed the best germination (46%) after the hot water operation in spite of the previous studies. We discovered that apparently there are close relationship between the seed fresh weight or water uptake capability and the percentage of infection. Following these recognition we modified our technique,in such a way that we fractionated the seeds based on their fresh weight/or relative density before we carried out the treatment. When we filtered the floating seeds on the surface of water, the hot water treatment was performed considerably better on the sunk seeds after separation. Therefore, by this special priming process we were able to reach 80% germination capacity of Virgina mallow seeds under laboratory conditions (26 oC without illumination).
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Rippl-Rónai’s color in the native parks: production and using of new, synthetic Wild sage color-mixture
59-64Views:151The climate changes is becoming more damaging to ornamental plants. Besides ornamental species and varieties of plants on public spaces. It will be necessary to use domestic, well adaptable climate-tolerant plant species.
One field of our growing ornamental plants researches from 2001 in the Centre of Agricultural Sciences of University of Debrecen, is studying drought patient, mainly Hungarian improved annual varieties, which are able to get acclimatized with the landscape. Moreover, we have in view to work out new, economical seedling production technologies. In the program, the excellent drought- and frost tolerant annuals and perennials belongs to the climate change tolerant plants. From the evaluated of species we want to create and spread the application. of the new types of ornamental horticulture culture.
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Will there be a horticultural triangle (cluster)? Thoughts about the reconstruction of the Hungarian horticulture between two European regions
21-27Views:99The authors of this study seek the answer to the question how to develop, in the first decade or decades of the 21st century, the university-level
horticultural scientific training, the horticultural innovation and the scientific co-operation between companies and universities in Debrecen and
in the North Great Plain Region and – in a wider sense – in Hungary to a standard being competitive even in European terms. With the synthesis
of the prospects of past, present and future, they drew the following conclusions. The reconstruction of agriculture, horticulture and food industry
is a part of reforming Hungary's countryside. Horticulture, producing high added value, will be able to decisively contribute to the plan whereas
the value presently produced in an agriculturally cultivated area of 1,000 euros/hectare can reach 2,000 to 3,000 euros in the next two decades.
A necessary and indispensable precondition to achieving this is the strengthening of the innovation output of the Hungarian horticultural sector.
Despite the numerous technical criticisms formulated in connection with the serious problems of Hungarian agricultural and horticultural
scientific innovation, no progress has been made in this field for the past one and a half decade. The scientific research of this topic hardly
continued or did not continue at all, the up-to-date surveys and in-depth analyses were missing. The objective, basic principles and tasks of the
Act CXXXIV of 2004 (TTI) enacted concerning research-development and technological innovation are clear and progressive. The co-operation
between the National Research Technology Office and the Hungarian Academy of Sciences, the setting up of the Innovation Fund are heartening
opportunities. These – along with the new Higher Education Bill to be passed – may as well be suitable for restarting the Hungarian agricultural
and horticultural scientific innovation. In our opinion, this requires a new, well-considered national agricultural programme, which can be
conceived in the framework of the "Ferenc Entz National Horticultural Plan" proposed by us for horticulture. In the most eastern Hungarian
university knowledge centre, at the University of Debrecen, the continuing of the horticultural scientific innovation strategy started in the last
decade may be the focal point and generator of the development of the so-called "Hungarian Horticultural Triangle”, or "Hungarian
Horticultural Cluster". This region comprises the Northern and Southern Great Plain Regions and the area between the Danube and Tisza
Rivers. Here, about 70 to 75% of the total Hungarian horticultural commodity stock is produced. The objective of the HORT-INNOTECH
DEBRECEN programme planned in 2004 by the University of Debrecen, Centre of Agricultural Science is to establish the horticultural scientific
research-development and technological innovation structure and knowledge base of the Hungarian Horticultural Triangle / Hungarian
Horticultural Cluster. In harmony with this, the objectives are to bring about competitive, new horticultural products, to improve the conditions
of utilising them, to enhance the competitiveness of enterprises based on technological innovation, to make use of the research-development and
innovation opportunities available in the regions in an efficient manner, to as full extent as possible, to encourage the creation of places of
employment producing high added value in the field of horticulture, to improve the technical skills of those employed in horticultural researchdevelopment and to promote their enhanced recognition by the society. -
Large Scale Measurement of the Total Antioxidant Capacity of Different hungaricum Fruit and Vegetable Samples with Photochem Chemiluminometric Device
300-304Views:78In our present study, we performed a quick test of the antioxidant capacity of domestic fruit and vegetable cultivars with a PHOTOCHEM® instrument (Analytik Jena AG, Germany). The instrument is special because it can measure both the total lipid-soluble antioxidant capacity (ACL method) and the total water-soluble antioxidant capacity. Another advantage of the system is its quick measurement. With similar instruments, such measurement can take several hours, while PHOTOCHEM® can perform it within a few minutes.
In our model studies, we examined the changes in the antioxidant capacity of sour cherry, green pepper, carrot, pumpkin, red beet and Brassica sp. cultivars. We aimed to determine how suitable the instrument is for quick, routine measurements in the case of the different horticultural products and which species have high antioxidant capacity values.
On the basis of our examinations, we can state that the PHOTOCHEM® system is suitable for routine, semi-automated measurements of the total antioxidant capacity at several vegetables and fruits. Besides this, the system can be used for testing the quality of food products, for monitoring storability in post-harvest technologies, and for special breeding programmes and for physiological and biotechnological studies. -
Effect of Selenium Supplementation on in vitro Radish and Green Pepper Seedlings Germination
149-155Views:132Selenium (Se) is an essential trace element for animals, microorganisms and some other eukaryotes. It has become increasingly evident that Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Although there is evidence that selenium is needed for the growth of algae, the question of essentiality of Se in vascular plants is unresolved. Therefore Raphanus sativus (Se accumulator) and Capsicum annuum (non Se accumulator) were treated with 0-200 mg/l natrium-selenate. The results showed that lower (2 mg/l) concentration natrium-selenate increased the fitomass and total antioxodant capacity in seedlings.
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Alternatives of the multiple use of Virginia mallow (Sida hermaphrodita L. Rusby) as a shrub-replacing mallow in the temperate climate zone
51-56Views:159In the seventies of the previous century, Dr. Zoltán Kováts set two directions in the research of mallows. One of the directions was the biotechnology of the mallow species and the other direction is using the mallow species as biomass material. In order to do this he brought mallow mother spawns of ornamental and biomass sorts from botanical gardens abroad and tested many of them, including the a Sida hermaphrodita kind. Fourty years later, for the second time this plant, known as the „energy mallow” got back to Hungary again, right into the sight of hungarian biomass business with the help of László Balogh and his associates using help from Poland. This genus got into the center of our research, because of it’s valuable attributes. The latest experiments are about using it as an energy plant, without examining genetic details. The plant grows up to more
than 3 meters, has high growing rate and produces big amount of green mass. We don’t have any hungarian data about whether the plant continues the sufficient growing rate or not, after cutting it back.
We have to explore the potentials in the Sida’s sublimation. The plant is mostly suitable for ornamental and energy utilization, so further sublimation should be aiming for these aspects. During my research period, we are willing to get to know these potentials and the best possible use of them. -
New color variations highlight Park sage (Salvia nemorosa L.) and characterization
41-44Views:132The negative effects of climate change on ornamental plants are also becoming more serious harm. In the current planting display appearing ornamental species and vareities needed in addition to the domestic well-changeable climate-tolerant plantspecies is involved. A natural populations of Sage Park is still unknown to us color- and shape variations are hidden. Plants of the natural vegetation collected from these clones formed three major evaluation of the color version with a temporary color variations have appeared. The axial length of inflorescens, color and brevity of the flowers were also significant differences. The botanists have not been tested by the sepal and bract from top to bottom and may vary. In 2011, the shoots are planted in field breading methods to perform an additional option.
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The bioactive compounds of sour cherry (Prunus cerasus) with special regards to antioxidant activity and antioxidant density
83-87Views:165Evidence suggests that a diet high in fruits and vegetables may reduce the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proven that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation.
In our work, we identified the total antioxidant capacity and ”antioxidant density” of sour cherry varieties named ”Újfehértói fürtös”, ”Debreceni bõtermõ”, ”Kántorjánosi” and ”Érdi bõtermõ” and those of the ”Bosnyák” sour cherry clones. ”Antioxidant density” is a biological value indicator obtained in a synthetic way, which indicates the antioxidant capacity of the particular food, e. g. fruit and vegetable, per 1 Calorie. -
The development of the scientific bases of horticulture and the history of horticultural innovation in the Northern Great Plain region and in Debrecen
17-20Views:75It can be told about the second half of the XXth century that, apart from preferring the large-scale growing of field plants and the largescale
livestock farming, corresponding to the central political will of the communist era, the significance and innovation output of
horticultural education in Debrecen was rather of follow-up trait, of secondary importance. The Tobacco Research Institute continued the
research work, and then even this activity was stopped. According to a survey finished in 1997, the mentioned institute had no invention,
granted patent, protection for registered model or any application for patent in progress at the Hungarian Patent Office. Until this time, invention activity at the University of Agricultural Sciences was of medium standard. In the National Patent Office, seven patent applications related to agricultural production and nine patent applications for other fields submitted under the inventors’ names were recorded. In the same period, the Cereal Research Institute (Szeged) led the absolute innovation list of Hungarian agricultural R+D institutions and university education institutes by submitting 164 own patent applications. Both in domestic and international terms, the horticultural innovation conducted at the University of Agricultural Sciences, despite the individual research results and achievements deserving recognition, without appropriate background – remained unnoticed. Let us put it this way: for the past decades, the light of the Debrecen Flower Carnival has not been thrown on the horticultural teachers and researchers of Debrecen. -
Deproteinized plant juice as part of circular economy: A short review and brief experimental data
23-26Views:1483As the population of the Earth is constantly growing it generates an unmet demand for protein, which is an urgent problem. The protein extraction process is a potential solution, which offers high-quality plant protein suitable for animal and human nutrition at a favorable price. The process used within our project produces green juice from the green alfalfa biomass through pressing. After the coagulation of protein from this green juice, the by-product is called DPJ (Deproteinized Plant Juices) or brown juice. Our preliminary results match the international literature, namely that brown juice take up as much as 50% of the fresh biomass in weight. To utilize this by-product is a crucial part of the process to make it environmental-friendly and financially viable as well. The examined brown juice samples came from a small-scale experiment of alfalfa varieties carried out in the experimental farm at the University of Debrecen. According to our preliminary results, brown juice has high macro- and micronutrient values, furthermore, it has a potentially high amount of antioxidant compounds. The study highlights that brown juice is suitable as an ingredient in microbiological media, in plant nutrition as a supplementary solution, for feedstock and for preparing human food supplements or functional foods. The potential utilization of all biorefinery products makes it a very appropriate technology for today’s challenges.
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Penicillium chrysogenum antifungal protein (PAF) production in transgenic tobacco (Nicotiana tabacum) plant
77-82Views:74Under the „Molecular farming” research program (product vaccines and substances for medical use with gene manipulated plant) in 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture Department of Plant Biotechnology experiments were launched to transform tobacco plant by PAF antifungal protein. Our aim was to learn the transformation technics. We chose the
Nicotiana tabacum and PAF as model systems.
Our work was to express several different paf constructions in plants with nuclear and plastid transformation too. After that we confirmed the presence of paf gene in the level of DNA and RNA. -
Anti-Oxidants in Agriculture
195-200Views:81Today’s programmes aiming at enhancement of fruit and vegetable consumption have been intensified. In the unanimous view of experts, different health problems, such as those of the immune system, inflammations, and even certain cancerous diseases can be prevented and/or cured with regular consumption of fresh (raw) fruit and vegetables. It is well-known fact that among the biologically valuable components, antioxidant compounds – C- and E vitamins, as well as carotinoides – play an important role. In this field, Hungary can expect success in the future since it has excellent plant genetic stock. Regretfully, national data banks regarding cultivation technology, cultivation areas or varieties for fruits and vegetables and their antioxidant contents do not exist in Hungary. Nevertheless, in connection with the so-called “Hungaricums” its existence would be of urgent necessity. Such excellent Hungarian products are – among others – a lot of sour cherry varietiles, the Szeged green pepper and the Makó onion. They enjoy high priority as “Hungaricums” even in the European Union and such activities that support these kinds of products should be enhanced by intense and consequent research work, which may prove their role as functional foods. Presently’ the USA leads in the research of antioxidant compounds of sour cherry, and so far 17 of these compounds have been found partly in Hungarian varieties. Similar research on green, and ‘pritamin’ peppers have not gone so far since they were limited only for seasoning paprika. In Hungary, studies on onion and garlic have not been performed. It should be mentioned that due to the continental climate, these products may be cultivated, consumed or processed only in a limited period. Taking this into consideration, processing and conserving methods are needed which make the consumption of these fruit and vegetables as functional food possible year-round. Scientific establishment of this set of questions is of current concern, because consumption of these products could have an important role in improving the health status of the Hungarian population in the future.
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Antioxidant density of Hungarian kapia and red bell pepper varieties
80-84Views:128There is a lot of evidence that the pepper is one of the most important sources of vitamin C. Albert Szent-Györgyi was the first to extract ascorbic acid from a red bell pepper in the 1930s. Previously people had eaten vitamin C on the long voyage to protect against scurvy. Vitamin C is an important antioxidant, and it is a cell protector today. Vitamin C protects the cardiovascular system against infection. This vitamin decrease blood-pressure, a raises the HDL cholesterol level (good), and inhibits gout.
„Antioxidant density” is a biological value indicator obtained in synthetic way. „Antioxidant density” indicates the antioxidant capacity of a particular food, e. g. fruits and vegetables, related to 1 Calorie. In our study, we measured the total antioxidant capacity, vitamin C content and „antioxidant density” of Hungarian paprika varieties. -
Adopt the tobacco plastid transformation technics in Hungary
73-76Views:69Under the GENOMNANOTECH Debrecen Regionális Egyetemi Tudásközpont (GND RET) research program in summer 2007 in UD Centre for Agricultural Sciences and Engineering Faculty of Agricultural Science Institute of Horticulture experiments were launched to transform tobacco plant by plastid transformation technics.
Our aim was to adopt first in Hungary the tobacco plastid transformation technics, which were used in Waksman Istitute Rutgers, The state University of New Jersey (USA) leading with Prof Dr. Pál Maliga. Scientists won scholarships learn and use this technics in the University of Debrecen. -
Micropropagation of Rudbeckia hirta L. from seedling explants
53-59Views:81We conducted experiments for developing an in vitro micropropagation protocol starting from meristems of Rudbeckia hirta L seedlings. We pre-soaked the seeds in sterile ion-exchanged water for 17 hours, and then achieved surface disinfection in two separate steps. First, we used concentrated household sodium-hypochloride solution for 20 minutes and, also for 20 minutes, we applied hydrogen peroxide of 10%, which was followed by washing with sterile ion-exchanged water three times. For the propagation of seedling meristems, the combination of half-strength solid Murashige and Skoog (1962) culture medium containing 10 mg/l of kinetin and 2 mg/l of kinetin + 0.1 mg/l of 2iP proved to be the most suitable. The average number of shoot-buds developed from the seedling axillary meristem in the best culture media varied between 5 and 17. Without separating them, we inoculated the shoot-bud clusters on MS culture medium containing 2 mg/l of IAA. After four weeks of incubation, we obtained elongated shoots, which we separated and inoculated into a new culture medium and from which we obtained elongated roots. The rooted plants were gradually acclimatised in the cultivation room, potted and carried to a greenhouse, and then planted in open field for subsequent observation. By adopting this method, our laboratory started the micropropagation of the superior and/or elite genotypes of the Rudbeckia hirta L. being of special value in respectt to breeding.