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Effects of initial spacing on the stand structure and yield of young black locust (Robinia pseudoacacia L.) stands
37-39.Views:180The choice of the right initial spacing of stands is one of the most decisive operations of a successful afforestation. It is even more important in the case of fast growing tree species grown in plantations; it is expressed in their early phase of development and in wood quality. The results of a 5-year long experiment with four treatments will be presented in this paper. They proved the priority of an initial spacing of 1.61.0 m in the majority of quality This treatment has been proved optimal exploitation of growing space by the young trees.
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Eicosapentaenoic acid production by Phaeodactylum tricornutum under different culture condition
133-136.Views:141Phaeodactylum tricornutum UTEX 640 strain of microalgae was screened under different culture conditions for their capacity to produce eicosapentaenoic acid (EPA) the most abundant polyunsaturated fatty acid (PUFA). In our experiments, the effect of sodium chlorid, nitrogen source, phosphate, initial pH, as well as the CO2 content of the medium on production of the eicosapentaenoic acid (EPA) by P. tricornutum were investigated. The EPA content of biomass was enhanced by the low pH of the medium, with increased concentrations of B17 vitamin and nitrate, and also with decreasing concentrations of sodium chlorid. The EPA is most likely associated with polar (membran) lipids and the role of EPA appears to be involved with membran permeability in microalge. The synthesis of phospholipids, enhances the EPA content of the cells, as expected. The maximum EPA yields were observed under optimum culture condition 43 — 48 mg/g of dry cell weight.
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Results of cherry rootstock evaluations in Hungary
11-14.Views:258The paper gives a review on the results of the latest rootstock evaluation projects in Hungary. Several cherry rootstock evaluation projects were carried out in Hungary during the last two decades. The evaluated rootstocks are partly mahalebs selected in Hungary, but more or less all the most important new rootstocks from different countries are involved. The aim of these evaluation projects was to find appropriate rootstocks in a wide range of vigour for our climate and soil conditions. The conclusion of the last 20 years of research in Hungary proved just the opposite of believes, that high density orchard can only be planted with dwarfing rootstocks. Before choosing the right rootstock the most important is to consider adaptability, precocity and productivity. Dwarfing and semi-dwarfing rootstocks showed proper results only with irrigation or very good site conditions. These rootstocks are very precocious, but branches form easily blindwood. This must be corrected by severe pruning in the first years. The relatively small leaf surface area can also be disadvantageous, but it can be controlled by pruning, fruit-thinning, irrigation and fertilization. After studying different rootstocks semi-vigorous rootstocks seem to be the most adequate for different site conditions in Hungary. Fast initial growing and competent precocity are their favourable characters. From the vigorous group of clonal mahalebs or seedlings are highly recommended first of all for poor site condition (sandy, lime soils with high pH). They turn to bearing early and easy to find them in the Hungarian nurseries. But on vigorous rootstocks trees might need stronger summer pruning.
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Comparative investigations on protoplast culture of some Brazilian and Hungarian sweet pepper cultivars and hybrids
39-45.Views:198Cotyledon protoplasts were isolated from 16-18-day-old in vitro grown seedlings of 9 Brazilian and 3 Hungarian pepper varieties and hybrids. Large numbers (average 9.59 X 106 protoplasts g 14 fresh weight) of highly viable (average 87.0%) protoplasts were released using a pectocellulolytic enzyme mixture. Protoplasts were cultured in K8p mediuni using an alginate disc embedding method. The osmotic pressure of the medium surrounding the alginate-embedded protoplasts was reduced by replenishing the liquid medium at K8p:K8 ratios of 1:0. 2:1, 1:1 in the first. second, and third week, respectively. Initial plating efficiency (IPE) average was 38.5% and after 21 days protoplasts reached microcolonies (15-20 cells) stages. Microcolonies were transferred after 3-4 weeks to a MS-based medium supplemented with 1.0 mg I-1 zeatin, 3.0% (w/v) sucrose, 0.24% (w/v) phytagel and pH 5.8, whereupon they formed callus. Final plating efficiency (FPE) average was 0.29% at a plating density of 1.0 x 105 protoplasts Protoplast-derived calli were cultured on a range of MS-based media supplemented with either BAP, IAA, TDZ; and zeatin. No morphogenic response was observed in any genotype investigated.
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1-MCP and STS as ethylene inhibitors for prolonging the vase life of carnation and rose cut flowers
101-107.Views:262The effect of STS and 1-MCP on the postharvest quality of carnation and rose cut flowers was studied. Cut flowers of Dianthus c..aryophyllus L. cv. Asso and Rosa hybritia cv. Baroness were treated with silver thiosulfate (STS) at 0.4 mM with sucrose at 50 g 1-t and 1-methylcyclopropene ( I -MCP) at 0.5 g m-3 for 611.
Pretreatment with STS and 1-MCP significantly extended the vase life and minimized the % loss of initial weight of carnation and rose cut flowers comparing to the untreated control. The two chemicals applied inhibited the chlorophyll degradation and carbohydrate loss and hence, significantly improved the postharvest quality of carnation and rose cut flowers comparing to the control. Ethylene production by cut flowers was inhibited as a result of using these chemicals. In general, there were no differences between STS and (-MCP but the later does not have the heavy metal implications of STS treatment, and hence, using 1-MCP pretreatment for extending the vase life of carnation and rose cut flowers was recommended.
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Growing characteristics of apple cultivars in environmentally friendly growing systems
7-10.Views:255Nowadays the condition of the good saleability of the fruit is the application of controlled environmentally friendly technologies. Success of the growing is basically influenced by the production value of the cultivars, and their flexibility to the different technologies. In our examinations the effect of the integrated and organic farming system has been evaluated on the growing characteristics of the apple cultivars. According to our results the trunk thickness of the trees both in the initial and both in the later bearing period is higher in the integratedproduction compared to the organic farming system. This differences most likely caused by the variance of the crop load.
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Stress physiology of palm trees II. The effect of heavy metals and high irradiance on the photosynthesis of palm Trachycarpus fortunei
84-88.Views:164A study was carried out to analyse the individual and combined effects of heavy metal toxicity and high irradiance on the photosynthetic characteristics of young, fully expanded leaves of palm seedling Trachycarpus fortunei under laboratory conditions. Heavy metals were found to inhibit both the light and dark reactions of photosynthesis and the inhibition was more affected in the light than in the dark. Single photoinhibitory conditions caused a 60 % decrease in the electron transport activity after 120 min of light exposure which was completely reversible in the dark. In contrast, the combined effect of high light and heavy metal treatment resulted in a 90 % decrease in the activity, but no reversible recovery in the dark could be detected. This indicated that the simultaneous effect of these two stress factors led to irreversible damages of the photosynthetic machinery and as a consequence caused the general destruction of the plant.
Abbreviations and symbols: Fo: initial chlorophyll fluorescence; Fm: maximum total fluorescence; Fv: variable fluorescence; AFi: intermediate level of fluorescence induction; PSII: photosystem 2.
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Rootstocks for Cherries from the Department of Fruit Science Budapest
63-66.Views:196Cherry rootstock breeding started at the Department of Fruit Science, SSU Budapest by the late 50-s and the activity can be divided into three main groups. In the first stage the activity was focused on collection of native mahaleb cherry (Prunus mahaleb L.) varieties lead by L. Sebők. After evaluation in the nursery and orchard tests there are four promising rootstock cultivars selected from this material: 'Korponay' used as self fertile seed tree, its seedlings are recommended for sour cherries. The mahaleb varieties 'Bogdány' (vigorous), 'Egervár' and 'Magyar' (medium vigour) are propagated by cuttings. The next project has started in 1979 with the aim to select self fertile mahaleb seed trees producing homogeneous seedling populations with reduced vigour. Inbred populations from isolated flowering self fertile trees were produced and planted out in 1980. The inbreeding of 'Korponay' self fertile P. mahaleb variety resulted in specimens with different fruit colour (yellow, red, black), fruit shape and size. From among them self fertile trees were selected with various growth characteristics. Seedlings of that self fertile mother trees (S2 population) were tested in seedbed, they showed homogeneous phenotype characteristics as liners in the nursery. As rootstock of 'Érdi bőtermő' sour cherry in the orchard most of the S2 lines proved to be less vigorous in comparison to SI popuplations. 'Érdi bőtermő' trees budded on certain S2 lines in the orchard are more productive than those on S1 ('Korponay' seedling). Characteristics of the S2 generation as seed tree were studied as well. We expect to get morphologically homogeneous seedling populations with different growth vigour and good productivity in the later inbred generations. In the last couple of decades the research activity concerning ground-cherry and its hybrids resulted in dwarfing rootstocks. Prunus fruticosa Pall. hybrids from the natural flora of Hungary were collected and artificial hybrids were created between P. fruticosa and mahaleb cherry. Most of them are in the initial tests, only one of them is before registration, named 'Prob', which is a dwarf rootstock for sweet cherry. By the screening of new hybrids medium vigorous or semi dwarfing and precocious rootstocks seem to be promising for the cherry industry.
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Effects of different levels of NaCl and CaCl2 on seed germination characteristics of Melissa officinalis L. and Ocimum basilicum L.
21-25.Views:325More than 15 million hectares of Iran’s land areas are suffering from salinity.We investigated the effects of two types of salt (NaCl, CaCl2) in different levels on the germination and initial growth of two medicinal and aromatic plants, Melissa officinalis L. and Ocimum basilicum L. The experiment was carried out in Qazvin Agriculture and Natural Resource Research Center based on a randomized complete block design, for 14 days in a germinator. Sweet basil germinated above 50% in each saline treatment (EC=10 dSm-1), in alkali treatment (CaCl2=18800 mg/L) and in sodic-saline treatment (Ec=10 dSm-1; SAR=5.6), however at the highest concentrations germination was slowed down. Lemon balm showed a higher sensitivity: except the lowest NaCl treatment, each other ones inhibited germination and its rate to some extent. The combined treatments of both salts increased the stress reactions. However, seedling growth was less effected. The results showed a stimulating effect of NaCl treatment in 2500 mg/L (EC<5 dSm-1) for both species concerning germination and seedling growth.
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Grape rootstock - scion interactions on shoot growth and cane maturing
30-34.Views:214Interaction between the rootstock and scion varieties (clones) was examined. Berlandieri x Riparia T. K. 5BB, Berlandieri x Riparia T.5C being the most widely used varieties in Hungary, Fercal, Ruggeri 140 being recently used on special soil conditions ; Georgikon 28 a new established variety and Berlandieri x Riparia T. 8B GK 10 clone were the rootstocks used. The V. vinifera L. varieties were a clone of Italian Riesling and two new hybrids from the same cross population f ( Noble Italian Riesling X Ezerjó) X ( Noble Italian Riesling X Pinot Gris ) }. This study presents the results how the growth habit depends on different scion/stock combinations in the first three years.
Shoot growth characters were observed during the initial years after plantation in the vineyard until we got the first yields. Our results did not agree with those of Pospisilova (1977) that the anticipated vigour of shoot growth will appear later in the vineyard only. However we agree with Zimmerman (1970), who showed that the differences are caused by the rootstocks in the growth habit of vines during the first two years already. The differences in shoot elongation decreased similarly to the rootstocks in the first year of fruit production. It shows us that the intense root development caused vigorous shoot growth, which we think to be a rootstock effect. When the vegetative and generative development are balanced already, the effect of scions in shoot elongation became stronger than the effect of rootstock.
We also found great differences in the maturation of wood each year from planting of the vineyard to the year of fruit production.
During the first years the differences of cane maturation were greater according to the scion varieties, the means were significantly different. In the first ripening year the differences dwindled or increased due to the rootstocks. This means that the effect of the rootstock on cane maturation became stronger in the first year of fruit production.
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The role of black locust (Robinia pseudoacacia L.) in establishment of short-rotation energy plantations in Hungary
41-44.Views:286Establishment of short-rotation energy plantations for fuel production has been of international interest for many years. Energy plantation experiments in Hungary have been conducted for a longer time. In the country black locust ( Robinia ps eudoacacia L.) is one of the most important stand-forming tree species, covering approximately 23% of the forested land (410 000 ha) and providing about 19% of the annual timber output of the country. This fast growing species seems to be suitable for energy plantations as well. So, in Helvecia (Central Hungary, sand-soil region) two energy plantation s were established u sing common black locust and its cultivars improved in Hungary. The spacing variations of the common black locust were: l.5x0.3 m, I .5x0.5 m and l.5x 1.0 m. At the age of 5 the closest spacing ( 1.5x0.3m) produced the greatest annual increment in oven-dry weight (6.5 t ha·1 yr- 1). In the trial with black locust cultivars planted in spacing of 1.5xl.0m, at the age of 7 the highest annual increment in oven-dry mass was produced by the cultivar ' Ulloi' (9.7 t ha-1 yr- 1) followed by the common black locust (8.4 t ha-1 yr- 1) and the cultivar 'J tiszkiseri (1.6 t ha·1 yr- 1). The trials have verified that in temperate climate the increment in oven dry dendromass of black locust energy plantation s has ranged from 6 to 12 t ha·1 yr·1. On the basis of the trials' evaluation the quantity of dendromass mostly depends on site quality, species and cultivars, as well as on the initial spacing (plants per hectare).
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Effect of acidity on growth rate and stroma formation of Monilia fructigena and M. polystroma isolates
63-67.Views:197The effect of acidity (pH) ranges on the mycelial growth and stroma formation of Monilia fructigena Pers: Fr. and of M. polystroma van Leeuwen was determined on agar plates and apple fruits. Four isolates of each of the brown rot fungi and two apple cultivars, `James Grieve' and 'Cox's Orange Pippin', were used for the study. For the agar plate study, a range of the initial pH was prepared from 2.5 to 6.5. The dishes were inoculated with a 4 mm plug of each isolate and incubated at 23 °C in darkness. The mycelial growth was measured after 1.5, 4, 7, 10 and 20 days of incubation. After a 30-day incubation, stroma formation was determined by image analysis and weighing of mature stroma. In the fruit experiment, both cultivars were inoculated with one isolate of M. fructigena and of M. polystroma. The pH changes were determined after 7, 14, 28 and 35 days of incubation in both healthy and inoculated fruits. The fastest mycelial growth was at pH 4.5 for M. polystroma and at pH 3.5 for M. fructigena. After a 30-day incubation, M. polystroma isolates produced twice or three times more stroma compared to M. fructigena isolates. For both brown rot fungi, the amount of mature stroma increased from pH 3.5 to 5.5, and then decreased at pH 6.5. Results of the.fruit experiment showed that healthy fruits were quite acidic (pH < 3.5), but pH rapidly increased in the inoculated fruits for both cultivars, reaching pH 4.6-5.4 depending on cultivar and fungus isolate. On both cultivars, the stroma developed at a significantly higher pH for M. polystroma than for M. fructigena. Biological and practical implications of the results are discussed.
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Extending the vase life of Solidago canadensis cut flowers by using different chemical treatments
83-86.Views:235In order to increase the vase life as well as quality of leaves of goldenrod (Solidago canadesis), the effect of 8-hydroxyquinoline sulphate (8-HQS), silver thiosulphate (STS) and l-methylcyclopropene (l-MCP) were investigated. 8-HQS was used as a continuous treatment at 400 ppm with or without sucrose at 50 g/l. The treatment of STS was used by putting the flower bases at 0.4 mM for 6h with or without sucrose at 50 g/l. l -MCP was used at 0.5 g/m3 for 6h dry or in water. Except the treatment of l -MCP in water, the chemical treatments, which were used, led to the increase vase of life of leaves as well as to the inflorescence of cut solidago spikes compared to the control. The best treatment in this concern was 8-HQS at 400 ppm without sucrose, which resulted in longest vase life of leaves as well as inflorescences and lowest percent loss of fresh weight of initial.
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Preliminary studies on propagating natural mason bee (mixed Osmia cornuta and O. rufa) populations in artificial nesting media at the site for fruit orchard pollination
95-101.Views:291Preliminary field management studies were made to increase the population density of native early spring Osmia species (O. cornuta, O. rufa) at fruit tree plantations in Hungary. Initial bee activity around artificial nesting media and the population increase during consecutive years were much smaller at large commercial fruit orchards under intense chemical plant protection than in small mixed fruit plantation with moderate or low pesticide usage. Osmias accepted all the four kinds of tested artificial nesting media (reed, bamboo rods, hardwood blocks and light walling blocks with drilled holes). Reed provided the best nesting conditions so it is suggested to be used in the practice. Calculations showed that under favourable conditions more than 50 to 100 thousand incoming flights (arrivals or landings) occurred in a single day around a single bee shelter and this makes enormous number of possible bee visits at fruit tree flowers. For this reason it is strongly recommended to put simple bee shelters filled with artificial nesting media into fruit orchards. Experiences show that the population sizes of Osmias increase during consecutive years without any specific additional maintenance except providing shelters and nesting media.
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Aminoglycoside antibiotics affect the in vitro morphogenic response of chrysanthemum and tobacco
93-104.Views:119Broadly the success of genetic transformation of plants requires non-chimeric selection of transformed tissues and its subsequent regeneration. With rare exceptions, most plant transformation protocols still heavily utilize antibiotics for the selection of transgenic cells containing an antibiotic-degrading selectable marker gene. The morphogenic capacity of in vitro chrysanthemum and tobacco stem and leaf explants change with the addition of aminoglycoside antibiotics (AAs). Of 6 antibiotics tested, phytotoxicity occurred at 10-25 and 50-100 pgml-I in chrysanthemum and tobacco explants, respectively, depending on the size of the explant and the timing of application. The presence of light or darkness also had a significant effect. The use of transverse thin cell layers (tTCLs) in conjunction with high initial AA selection levels supported the greatest regeneration of transgenic material (adventitious shoots or callus) and the lowest number of escapes. Flow cytometric analyses demonstrate that regeneration can be predicted in both species, depending on the ploidy level of the callus. Endoreduplication was not observed in chrysanthemum, even at high AA levels, but occurred (8C or more) in tobacco callus, even at low AA concentrations (5-10 pgml-1). The higher the AA level, the greater the DNA degradation and the lower the 2C and 4C values.
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Genetic variation in the rose pathogen Marssonina rosae estimated by RAPD
63-67.Views:183Blackspot, caused by the fungus Marssonina rosae (Diplocarpon msae), is one of the most devastating and widespread diseases in garden roses, and it has as yet not been fully characterized in molecular terms. In this initial study we used RAPD analysis to investigate the genetic diversity among and within a few geographically diverse groups of single-spore isolates of M. rosae. DNA was extracted from in vitrogrown mycelia of 1 I single spore isolates grown on PDA medium. High levels of polymorphism were detected among the isolates. They clustered into three distinct groups: Group !consisted of isolates from eastern North America plus a European isolate (Germany), Group 2 included isolates from southern Sweden, and Group 3 included the isolates from Manitoba, Canada. The greater similarity of the environmental conditions in eastern North America and Europe as compared to the Canadian prairies suggest that. climate and weather could be key factors in influencing the potential race structure of M. rosae. However, variations among closely situated sites, e.g. southern Sweden, also occurred.