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Impact of sodium-selenate on the growth of radish (Raphanus sativus L.) seedlings in vitro
113-115.Views:208Selenium (Se) is an essential trace element for animals, microorganisms and some other Eukaryotes. It has become increasingly evident that Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Although it is well known that some species among higher plants are able to accumulate selenium in their tissues, but others are not able to do so, and there is evidence that selenium is needed for the growth of algae, meanwhile the question of essentiality of Se in vascular plants is unresolved. We aimed to study the in vitro growing and to characterise some physiological properties in radish (Raphanus sativus L.) seedlings treated with 0 to 200 mg/1 sodium-selenate. The results showed that lower (2 mg/1) concentration sodium-selenate increased the biomass as well as the total antioxidant capacity of seedlings. The seedling's selenium content showed linear correlation with the sodium-selenate content of the medium.
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Total anthocyanine content and antioxidant density of some Hungarian sour cherry varieties
109-111.Views:434Evidence suggests that a diet high in fruits and vegetables may decrease the risk of chronic diseases, such as cardiovascular disease and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proven that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation. In our work, we identified the total antioxidant activity, anthocyanine content and "antioxidant density" of sour cherry varieties named "Újfehértói fürtös", "Debreceni bőtermő", "Kántorjánosi" and "Érdi bőtermő" and those of the "Bosnyák" sour cherry clones. "Antioxidant density" is a biological value indicator obtained in a synthetic way, which indicates the antioxidant capacity of the particular food, e. g. fruit and vegetable, related to 1 Calorie.
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Down-regulation of ethylene production in carnation (Dianthus Caryphyllus L.) by an apple derived ACC-cDNA
101-104.Views:140Transgenic carnations were produced with an apple derived antisense ACC-synthase cDNA. Transgenic carnation regenerants were potted in glasshouse. All transformed plants showed normal growth and were true-to-type. Ethylene production — measured at full opening stage — lowered by 30-60 %, no plant with 100 % decrease was identified. The vase-life has been observed for 5 years. 38 % of the transformant carnations showed a higher a relative value in days by more than 2 days to 6 days. Twenty six plants were found exhibiting the most marked alterations in the tested trait. In these plants ethylene production decreased by 37-67 %, they have longer vase-life (by 4 days or more). Since the fragrance variety 'Bíbor' was the plant material for genetic modification of vase-life, this trait has been conserved after transformation in spite of the fact that the position of transgene integration cannot be directed.
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Organoleptic evaluation of sweet cherry varieties
7-13.Views:672The extraordinary abundance of precipitation in 2010 (somewhere around 1000 mm) influenced highly the development of taste and flavour in cherry fruits. As stated, only a few varieties out of 27 were acceptable as for commercial quality. Under the given climatic conditions, actually ‘Bigarreau Burlat’ earned 965.11 points and proved to be the best in the early ripening group. In the mid season group, three varieties earned more than 1000 points (‘Giant Red’, ‘Carmen’ and ‘Vera’ grown at Nagykutas and Pallag), whereas in the late ripening group ‘Germesdorfi’ sent from Csenger (1084), ‘Linda’ (1070.07) and ‘Lapins’ (1052) received recognition. Correlation has been tested on the basis of 27 varieties between the individual properties. We sated that the most important attributes, which influenced the general impression of decision makers are the following: form and size of the fruit (r=0.835 and 0.797), furthermore juiciness (r=0.776), taste (r=0.876) and sweetness (0.875). Crispness was considered to be typical to cherry (r=0.743). Relation between acidity and sweetness was also essential to determine the acceptance of the cherry character.
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Actual state of research concerning vitamin C as reflected in the literature (Review article)
7-15.Views:222Vitamin C (L-ascorbic acid) being essential for many living organisms, including man, became once more into the focus of interests because of its numerous physiological effects. Its anti-scurvy and anti-oxidant properties have already been recognised since long in the human body, but it turned out gradually that it has many other functions. In plants, its primary importance is defense against the photo-oxidative stress.
The present review is intended to reveal some details of the artificial synthesis of vitamin C. Emphasis is put on the metabolism of L-ascorbic acid in higher plants. Biosynthetic processes, translocation and accumulation are discussed in detail on the basis of recent results published in the scientific literature.
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Flower density and winter damage of apricot and peach varieties
53-56.Views:291Hungary is located on the northern boundary of economical apricot and peach production. The present assortment of varieties and the actual, not adequately selected growing sites cause a permanent risk of winter and spring frosts in their cultivation. The field observations are performed at Debrecen, the Experiment Station Pallag, on 20 apricot and 21 peach varieties. The flower density among the varieties attained 3-4-fold differences. Three categories have been suggested for both species. The density was inferior in Hungary established varieties compared with the new varieties of foreign origin. The minimum temperatures of January 9, 2009 was –17,6 °C , and of December 21, 2009 also –17,6 °C. In some varieties the damage of buds attained 100%. For estimating the yield security, we need to consider also the flower density and the frost damage together. For a mediocre yield, we need a flower density in both species of at least 0.2 living bud/cm. The results confirm the statement that in Hungary, the revision of growing sites is indispensable in order to develop a profitable and competitive apricot and peach growing industry.
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Hungarian fruits and vegetables of high anti-oxidant activity as functional foods (Review article)
13-21.Views:442Recently, projects aiming to enhance the consumption of fruits and vegetables are intensified. Experts agree in the principle of fresh vegetable foods being a valid panacea in averting risks of ailments as well as curing immune-insufficiency, inflammations, moreover, certain cancerous processes. It is generally accepted that among substances of biological activity the anti-oxidant compounds such as vitamins C, E and carotenoids, etc. have the major role in this process. Hungarian agriculture has outstanding chances in utilising its natural as well as cultivated plant resources and favourable climatic conditions. It would be, however, necessary to build up a databank of antioxidant substances found in fruits and vegetables and including the modifying effects of technology, growing site, variety, etc. The concept of promoting the trade of Hungarian food-specialities as "Hungaricum" needs, urgently, the aid of a databank of that kind. Some of those excellent products are for instance the sour cherry, pepper and onion. They enjoy high priority as "Hungaricum" in the EU and it should be enhanced by intense and consequent research work, which may prove their role as functional foods. The USA is the leading country in research on the anti-oxidant substances of sour cherries, and up to now more than 17 compounds have been found in Hungarian varieties among others. In pepper fruits used as vegetable and source of vitamin C, the analyses are still lacking because research of the past concentrated on the products of milled spice pepper. Onion and garlic are entirely unexplored in this respect. It should be noted that availability of these fresh products in the moderate climate is restricted to a relatively short season. For that reason, some processing and preservation methods are needed in order to use those fruits and vegetables as functional foods all around the year. The scientifically founded endeavour as a solution of the questions mentioned is stimulated by vigorous commercial interests as well as by the urgent needs of the consumers to improve their health.
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High antioxidant - and anthocyanin contents of sour cherry cultivars may benefit the human health: international and Hungarian achievements on phytochemicals
45-47.Views:448Evidence suggests that a diet with high fruit and vegetable consumption may decrease the risk of chronic diseases, such as cardiovascular diseases and cancer, and phytochemicals including phenolics, flavonoids and carotenoids from fruits and vegetables may play a key role in reducing chronic disease risk. Recent research has proved that sour cherry (Prunus cerasus L.) is a valuable natural source of some bioactive compounds important in human health preservation. According to the published data, the most important biological effects of sour cherry are connected — directly or indirectly — to their endogenous antioxidant behaviour as well as to their specific pattern of anthocyanin components. In the present work, we measured the total antioxidant capacity of some Hungarian sour cherry varieties in combination with their anthocyanin-, and vitamin-C content. In 2003, twelve clones were selected and grafted from a local sour cherry population called "Bosnyák" sour cherry grown in small home gardens and farms of the village Csengod (Great-Plain Region, South Hungary). Other Hungarian sour cherry varieties, i.e. cv. Újfehértói fürtos, cv. Érdi bőterrnő, cv. Debreceni bőterrnő, cv. Csengődi and cv. Kántorjánosi served as a control.
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Production of transgenic carnation with antisense ACS (1-aminocyclopropane44-carboxy late synthase) gene
104-107.Views:186Dianthus chinensis and Dianthus caryophyllus varieties were tested for shoot regeneration from leaf and petal explants and transformed with Agrobacterium tuniefaciens strains (EHA 105 and LBA 4404) harbouring an apple derived ACS cDNA in antisense orientation in order to reduce ethylene production and influence the ethylene dependant traits in carnation. After transformation regenerating shoots were selected on MS medium containing 50-75-100-125-150 mg/1 kanamycin and supplemented with 1 mg/1 BA, 0.2 mg/1 NAA. Transgene integration was proved by PCR analysis with npt II spcific primers followed by Southern hybridisation of DNA isolated from green shoots on medium containing 150 mg/1 kanamycin. Several putative transformants were subjected to RT-PCR in order to examine the npt 11 expression at mRNA level. Both the transformant and the non-transformant plants were potted into glasshouse to observe the effect of changed ethylene production on flowering time, petal senescence and vase life.
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Strategy of the sour cherry verticum in the Northern Great Plain Region Hungary (Analytic study)
7-31.Views:294Sour cherry growing and consumption grows dynamically around the world. The present volume of 1 million tons will incerase within 10 years with 20-30, or even with 50%. In the world wide sour cherry production, Europe is a decisive factor, i.e. 2/3 of the volume is grown there. Prominent capacities are concentrated in East-Central Europe, mainly Poland, Germany and Hungary. In the future, new concurrent exporters are expected on the European market as Turkey, Iran, Serbia-Montenegro. Hungarian sour cherry production has rich traditions, so the growing techniques and the assortment of sour cherry varieties make Hungary a „Great Power" on this field. Fresh fruit and products developed from sour cherry represent values distinguished as „Hungaricum" on the markets. Sour cherry growing and the path of its products are one of the „pulling branches" of Hungarian fruit growing. Sour cherry occupies 15% of area for fruit growing and 40% within the stone fruits. Sour cherry was grown widely in Hungary, it was grown everywhere as for utilizing waste areas. This is the main reason that yields are low as a mean of 15 000 ha and the volume is low (50-60 000 tons) only. To that poor figure the heavy infections of Monilia contributed substantially in the last couple of years. The two most valid arguments of using the present varieties as the best solution are 1) the cross bred new varieties, and 2) the selections of local, traditional varieties, which substituted the earlier dominant 'Pándy meggy' variety, which had a good quality but yielded poorly. Sour cherry growing of Hungary shifted from the dry regions of the country toward the cooler and more humid regions, where the weather excesses secure a less risky production. The most decisive region is the Norther Great Plain Region comprising SzabolcsSzatmar-Bereg county, where more than the half of the Hungarian sour cherry volume is produced, and which is bound to increase its production in the future. The majority of sour cherry produced in Hungary is processed, moreover, an important fraction of the exported fresh fruit is also used by the industry. The main importer of Hungarian sour cherry is Germany. The industry manufactures mainly canned products, a smaller fraction will be processed to other products. The expected volumes of sour cherry grown in Hungary in the next 5 and 10-year-period was estimated from data based on the ratio of young plantations, predicted consequences of the global climatic changes, phytosanitary aspects, furthermore, on the development of the technological level. In the region, the volume grown within 5 years, 40 000 t/year will increase within 10 years to 55 000 t/y. The processing in Hungary is not sufficiently differenciated, which is attributed partly to the characters of the varieties, partly to the weaknesses of the processing industry. One of the reasons is the suitability of varieties mainly for canning products. Processed sour cherry products could not be sold at the same price levels achieved by concurrent sour cherry growing countries. The vertical structure of the path of products of sour cherry disposes of adequate processing capacity being ready to be developed or there is sufficient intention of making investments for the purpose of manufacturing special sour cherry products. Significant tasks of development are actual in the field of the ecological and biological conditions of production. Volume and yield security as well as the maturity time and diversification of processing possibilities are the main endeavours in widening the assortment of varieties to be grown in the near future. The main objective in growing techniques is the modernization of phytotechnical procedures, and new solutions of methods of mechanical harvesting and related technical innovations are necessary in the sour cherry verticum. A key question is the effectiveness of phytosanitary procedures with special reference to the Monilia fungus and to the cherry fruit fly as the most important pest. There are two points of break through in the Hungarian sour cherry verticum. On the one hand, meeting the increasing demands in fuits for fresh consumption, on the other hand, the diversification of processed sour cherry products and their introduction to the markets. Both are aiming to increase the competitiveness of the Hungarian sour cherry. For that purpose, outstanding varieties and excellent as well as internationally recognised fruit qualities are ready to be utilized. The most susceptible problems of the Hungarian sour cherry verticum are associated with marketing, alliance of the grower-and processor organisations and their co-operation because no overall integration within the sour cherry verticum has been established yet. Most urgent necessity as well as possibility of changes are felt in the Northern Great Plain Region.
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Effect of postharvest sodium benzoate treatment on some fruit parameters of two organic apple cultivars
35-37.Views:444The aim of this study was to investigate the effect of postharvest sodium-benzoate treatment on some fruit parameters of two organic apple cultivars (’Topaz’ and ’Florina’). Assessments were made at three times during storage: 17 November 2019, 20 December 2019 and 23 January, 2020. During every assessment dates, each fruit was observed separately, and determined the proportion of i) healthy fruits (%), ii) post-harvest fruit rot diseases iii) fruits with mechanical injury (%), iv) fruit with russetting (%), v) damage of codling moth and vi) Ca-deficiency symptoms. In addition, fruit weight (g) was measured at each assessment date. Sodium benzoate reduced the fruit decay and the proportion of healthy fruit was higher in this treatment compared to water treated fruit. This effect could be seen in all assessment dates and on both cultivars. Effects on other parameters were various according to cultivar and assessment dates. Loss of fruit weight was similar in both treatments and cultivars compared to control treatment.