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Effect of variety and cultivation technology on phenols and antioxidant activity of sweet and sour cherry
59-61.Views:227The goal of the present work was to compare different sweet and sour cherry cultivars and cultivation methods (bio/integrated) with respect to polyphenol content and antioxidant activity. The concentration of total polyphenols ranged between 880–1050 mg kg-1 of fresh fruit, whereas antioxidant activity expressed as TEAC was found to be between 5.4 and 10.3 mmol kg-1 for the sweet cherry cultivars examined. In case of sour cherry the level of polyphenols ranged between 1283 and 3490 mg/kg fresh edible part of the fruit. Antioxidant activity was recorded between 15–32 mmol kg-1 for the different sour cherry cultivars included in this work. After one-month storage at low temperature, the total phenols and antioxidant activity decreased by 2–40% in the sour cherry cultivars studied. The anthocyanin content in cherry cultivars was less (131–312 mg kg-1) than the135–1893 mg kg-1 found in sour cherries. Anthocyanin level was higher in samples produced under organic farming conditions than in those produced with integrated cultivation.
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Carotenoid composition and content in products of sea buckthorn and peach as determined by high-performance liquid chromatography
69-74.Views:216A study was conducted to analyse the carotenoids by high-performance liquid chromatography (HPLC) using reversed-phase cross linked end-capping and to study the pigment content and composition in sea buckthorn products as well as in freshly harvested fruits from peach thees grown under organic and integrated farming conditions. It was found that carotenoids in fruits of both crops occur mainly esterified with fatty acids in form of mono- and di-esters. The major carotenoids were esters of zeaxanthin, lutein, β-cryptoxanthin and β-carotene. The major carotenoids showed different response to processing of sea buckthorn being highly sensitive to thermal treatments such as blanching, cooking and drying. Significant differences were found between peach varieties in their carotenoid content, whereas the impact of organic farming on carotenoids formation was found to be variety-dependent. Two of the three varieties examined in this work, when cultivated under organic farming conditions contained lower carotenoid level as compared to that found in the fruits of the same varieties but produced in integrated farms.
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Growing characteristics of apple cultivars in environmentally friendly growing systems
7-10.Views:226Nowadays the condition of the good saleability of the fruit is the application of controlled environmentally friendly technologies. Success of the growing is basically influenced by the production value of the cultivars, and their flexibility to the different technologies. In our examinations the effect of the integrated and organic farming system has been evaluated on the growing characteristics of the apple cultivars. According to our results the trunk thickness of the trees both in the initial and both in the later bearing period is higher in the integratedproduction compared to the organic farming system. This differences most likely caused by the variance of the crop load.
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Comparison of frost damages in apple plantations cultivated with environmental friendly growing technology
21-24.Views:169The global changes in climate and meteorological conditions have many negative consequences, which may diminished with adequate measures. In our continental climate, the winter frosts but also the late spring frosts are always threatening. There are technologies to avoid late frost damages (with spray irrigation or with smoking), but against winter minima, we are nearly helpless. The different damages experienced recently called our attention on the question of optimal condition, which is a delicate balance between the vegetative activity and the provision of nutrients. The excesses on both ends are increasing the danger of damages (Zatykó, 1980). Our examination was performed in the plantation, which was cultivated according to ecological and integrated technologies. The variable condition of the trees gave opportunity to evaluate the effects of cold temperatures as frost damages. Our results will represent not only the differences between varieties but also the effects of the growing technologies – which influence the condition of the plants – consequently, the measure of frost damages.
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Apple powdery mildew caused by Podosphaera leucotricha: some aspects of disease management
29-33.Views:527Apple powdery mildew (Podoshphaera leucorticha) occurs wherever apples are grown. One of the most important fungal disease of apple which causing severe econimic loss on susceptible apple cultivars. This review focuses on the control of apple powdery mildew. The first part of the study provides details of novel aspects of non-chemical control approaches, including agronomic measures, mechanical and biological control options as well as essential features of apple cultivar resistance. After this, developments in chemical control options are described sperately for integrated and organic apple orchards.
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Prediction infection risk on the basis of weather-related factors and Erwinia amylovora colonization in apple and pear flowers
39-54.Views:156Current infection risk prediction models utilize environmental parameters and field records, but do not take into account the estimated inoculum potential within the orchard. The object of this study was to survey the accuracy of three simple prediction methods under Hungarian climatic conditions, which could easily be used by the farmers. We also tested whether the accuracy of infection risk predictions can be improved by taking into consideration the incidence and/or rate of flower colonization by Erwinia amylovora.
After preliminary investigations in 1999-2001, data concerning the weather-related infection risk were recorded in 5 apple and 1 pear orchards in 2002, and in 12 apple and I pear orchards in 2003. The weather data were processed by the easy-to-use risk assessment models of the mean temperature prediction line (MTL), Smith's Cougarblight 98C and Billing's integrated system (BIS), and by the MaryblytTM 4.3 computer-assisted model for reference. The population size of E. amylovora in the flower samples was estimated within an order of magnitude by PCR.
For all years and orchards tested, Maryblyt indicated 35 days on which there was an acute infection risk. The same days were indicated by all 3 methods in 23 cases (66%), 8 days were indicated by 2 methods (23%) and 4 days were indicated by 1 method only. A similarly good correlation was found for prediction of the date of the first massive infection risk: in 2003, for instance, there was a perfectly consistent prediction by all 4 models in 9 of the 13 participating orchards. A coincidental forecast was provided by 3 of the 4 models in the other 4 orchards.
The results indicate that any of the risk assessment models could provide an increased accuracy of the actual infection risk prediction if combined with an estimation of the incidence of Erwinia amylovora colonization in the open flowers. We found no convincing differences in the size of the epiphytic population in flowers of cultivars possessing high or low susceptibility to Erwinia amylovora.
We conclude that the easy-to-use methods tested could be used by the fanners to recognize weather-related risks, especially when coupled with an estimation of the proportion of the pathogen-infested flowers. This local prediction would provide rapid information (faster than the regional forecast systems) specifically for a given orchard.
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Ecological drought resistance and adaptability of apple varieties
113-122.Views:300For adequate yields in apple plantations, during the long growing period of the fruit primordia, one of the decisive factors is water supply. Indicators of stress are valuable signs for the diagnosis of drought and necessity of watering, i.e. planning the irrigation of plantations. The aim of the present study was to find reliable signs of water stress on apple trees and at the same time conclude on the drought tolerance of different varieties. The plantation of apple varieties grafted on various stocks and cultivated according to different systems (irrigated, non irrigated, integrate and biological) has been examined continuously by leaf analysis. Along the period of growing fruits, measurements were made in the field, then the leaf samples were analysed in the laboratory for composition of pigments, carbohydrates and antioxidants, as well as the histology of the tissues checked. Without irrigation, the mean leaf mass and the relative chlorophyll content (SPAD) of the variety Idared on M4 stocks increases beyond the values of 50 SPAD, whereas on M26 stock and integrated system, it declines. In the collection of varieties, grown according to the „integrated” system, during the development of fruits, ‘Gála’ and ‘Remo’ varieties have been affiliated to the “less susceptible” group regarding drought tolerance because of the leaf morphology, structure and content of SPAD and antioxidants. However, ‘Idared’ and ‘Jonagold’ belong to the “susceptible” group. To the same group are ranged the ‘Akane, Red Rome vanWell, Pink Lady’ varieties. Those varieties reacted to a short period of drought by increased production (content) of carbohydrates. Regarding changes of carbohydrate content ‘Greensleeves’ and ‘Ozark Gold’ varieties belong to the moderately water dependent (requiring) group. In the field, SPAD-tests facilitated the measurement of nutrient-uptake and incorporation, which is proved by the tight correlation between the data of SPAD and the increment of leaf weight (r=0.76–r=0.88), however, this depends on the variety too. SPAD is an indicator of water supply and is related with the density of stomata, cannot used for the selection of water-exigent varieties but for (drought) tolerant ones. In integrated culture, the (drought) susceptible varieties display (water-soluble) hydrophilic and lipophilic antioxidants (ACWand ACL resp.) the quantity of which may have some role in drought tolerance.
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Effect of frost damage on leaf macronutrient status of eight apple cultivars in integrated apple orchard in Eastern-Hungary
37-40.Views:204The year of 2007 was critical for fruit growers in the region of Easter-Hungary. Several orchards were suffered frost damages. Significant frost damage was also happened in the orchard of Tedej Rt., which caused total fruit failure in the orchard. Our study was conducted in the integrated apple orchard (Malus domestics Borkh.) established on a lowland chemozem soil in East-Hungary, to investigate how frost changes the mineral content of different apple species. Leaf samples were collected 100 days after full bloom both in 2006 and 2007. The concentrations of nitrogen, phosphorous, potassium, calcium and magnesium were measured in leaves. The absence of fruits caused a smaller accumulation of nitrogen and magnesium in leaves. Falling down of flowers and fruit sets hindered the translocation of the uptaken phosphorous, potassium and calcium towards fruit sets. Due to fruit failure the vegetative processes became dominant. Leaves larger amount of phosphorous, potassium and calcium stored. Besides the absolute element content, the ratio of the different elements was also determined. Majority of calculated ratios were removed from optimal values due to frost. Both absolute content of nutrients and their ratios pointed out that the frost damage significantly affected the uptake of nutrients and their storing processes.
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Epiphytic microbiota of apple in integrated and organic growing
85-89.Views:168The surface microbial contamination is of great interest in case of fruits, since they are they potential sources of spoilage or foodborne diseases. The aim of this work was to compare the epiphytic microbiota of food safety concern of different apple cultivars as a function of cultivation methods (organic versus integrated), production year and place. Investigating 47 samples it was found that the average microbial contamination of the apple surfaces was within a certain range,, practically independent of cultivar, growing area and year. The frequency distribution of the surface bacterial, mould and yeast counts did not show significant differences between growing technologies or varieties. The principal component analysis the samples ranked the apple samples into seven groups on the basis of their microbial contamination level, and the discriminant analysis proved the goodness of grouping. The grouping was independent from cultivars and growing methods. No foodborne pathogen Salmonella spp. and Listeria spp. were found on the surfaces of apples.
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Flower production of apple varieties grown by different environmental technologies
47-49.Views:148The flower production of four apple varieties have been observed grown in integrated and in ecological growing technologies during the spring of 2010. The trees produced in the last year (2009) nearly similar yields, so the effect of the technologies may causen differences in the production of flowers. According to our results, the ecological technology produced higher flower densities in the majority of variables, which was in relation with the vigor, branching and thickening of the trunks. The varieties observed displayed substantial differences in the appearance of fruiting structures of different age on the branches. This information may prove to be essential for the decisions to be made in timing and severity at the pruning operations.
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Effect of organic and integrated farming on carotenoid and tocopherol content of apricot fruits
15-18.Views:203In modern and healthy diets antioxidants play an important role providing natural defence against serious diseases. Therefore it is recommended to include fruits and vegetables having high antioxidant capacity in daily diet in a due course. Apricot is one of the fruits receiving an increasing attention in this field. This study was conducted to investigate the composition and content of fat-soluble carotenoids and tocopherols in different varieties of apricot using recently developed liquid chromatographic methods. Also it was aimed to compare organic and integrated farming in their effect on carotenoid and tocopherol content of the fruits. The results showed that apricot fruit are rich in vital carotenoids and bioactive tocopherols with significant variation between different varieties. The organic farming had favourable effect on the level of the major carotenoids and depending on variety this technology either increases or does not have significant influence on vitamin E content.
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Effects of DMI and strobilurin fungicide combinations on scab and powdery mildew incidence in apple production: a preliminary study
37-39.Views:214The aim of our study was to evaulate the effectiveness of four DMI and strobilurion fungicide combinations in a season-long disease management programme against apple scab and powdery mildew in two integrated apple orchards on cultivar Jonagold. The four spray programmes were: i) standard, ii) DMI dominant iii) strobilurin dominant, and iv) DMI and strobilurin dominant. Apple scab incidence as generally high in both orchards in the standard fungicide treatment plots ranged between 21.8 and 26.4%. Leaf scab incidence was higher in all treatments compared to fruit incidence. Scab incidence was generally higher at Mándok compared to Ófehértó. In both locations the lowest scab incidence was assessed in DMI dominant treatment ranged between 5 to 10.7%, which was not signifi cantly different from the DMI and strobilurin dominant treatment. Powdery mildew incidence was generally low in both orchards in all fungicide treatments ranged between 0.002 and 0.5%. Although there were no signifi cant differences among treatments, the lowest powdery mildew incidence was assessed in the DMI and strobilurin dominant treatment in Mándok and in the strobilurin dominant treatment in Ófehértó.
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Integration of terrestrial laser scanning and spectral canopy scanner in horticulture applications
15-18.Views:236One of the most difficult challenge in the everyday practice to describe the canopy growing of fruit trees in an orchard. The photosynthetic activity is the basic of the primer production of plants. The measurement of leaf area and determination of the photosynthetic activity could be occurred with some elaborated methods between experimental conditions. In this article we present such an integrated methodology, which is ideal to determine the geometric and spectral characteristic of fruit trees between field conditions.We have carried out laser scanning technology to investigate the geometric-topological characteristics and parallel the active infra-red sensor to collect spectral data about an apple orchard. The surveys were worked out in an intensive apple orchard with drip irrigation system, protected by hail net in Study and Regional Research Farm of the University of Debrecen near Pallag. This study shows the filtering and interpretation methods of created data. The produced high accuracy data can be directly used in the precision horticulture. It could serve as a guiding data to implementation a future “virtual horticulture”. Higher spatial and temporal resolution could help for a better recognition of water balance of orchards.
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Tending operation models for black locust (Robinia pseudoacacia L.) stands growing on sandy soils in Hungary
55-57.Views:204A more intensive integrated research and development approach to the work carried out on the growth on sandy soils of stands of black locust (Robinia pseudoacacia L.) has been adopted in recent years, revealing several factors influencing stand growth. The fact that certain ecological factors influencing fundamentally the growth of trees have become unfavourable in Hungary in recent years has led to the more extensive use of black locust in the course of afforestation and forest regeneration schemes. The study presents a new, simplified tending operation model for black locust stands and age, growing space and target diameter models suitable for qualitaty log production and for mass assortments. The simplicity of these practice-oriented
models may foster the qualitative development of black locust management in Hungary and in some other countries where this tree species may gain greater acceptance by landowners and the forest industry. -
Tomato (Lycopersicon esculentum Mill.) anatomical, physiological, biochemical and production responses to drought stress - A mini-review essay
40-45.Views:796The cultivated plant climate association in agricultural yields is getting expanded consideration with regards to changing climatic conditions. Abiotic stressors can lead to morpho-anatomical, physiological, and biochemical alterations in harvests, resulting in a significant loss of profit. A comprehension of ecological elements and their communication with physiological cycles is critical for improving agricultural practices. Drought stress is among the main natural factor affecting plant development, growth, and yield measures. Assessing the impact of environmental change and atmospheric variability on tomato crop output will require a thorough understanding of this stress element. The physiology, development, improvement, yield, and quality of the tomato crop are all affected by dry season stress. This mini-review essay presents the most prominent features about the effects of drought stress on tomato crop plant physiology and production, with specific highlighting for the complex relationship between drought stress, and nutrients uptake.
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Some aspects of disease management of cherry leaf spot (Blumeriella jaapii) with special reference to pesticide use
45-49.Views:470In this review, some aspects of disease management of cherry leaf spot (Blumeriella jaapii) are summarised with special reference to pesticide use. In the first part of the review, we show the non-chemical control approach (e. g. removal of fallen leaves, planting resistant cultivar) against leaf spot. In the second part of the review, the effect of pesticides including fertilizers (urea) and fungicides on cherry leaf spot are discussed. Special attention are given to the fungicides of copper, dodine, captafol, captan, benomil, chlorothalonil, sterol demethylation inhibitors (e.g. fenarimol, fenbuconazole, myclobutanil, tebuconazole), and strobilurins about their effectiveness against cherry leaf spot. In the final part of the review, possibilities of cherry leaf spot control are discussed in integrated and organic cherry orchards.
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Tending operation models for white poplar (Populus alba L.) stands growing under sandy soil conditions
125-127.Views:157Integrated research aimed to intensify the practices of afforestation especially of white poplar and grey poplar woods. A new, simplified tending procedure has been developed to substitute earlier models considering the target diameter by right spacing as a tool to achieve adequat quality of logs. The practice-oriented model may help the qualitative improvement of white poplar growing technology in Hungary as well as in Serbia.
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Chemical communication with volatile semiochemicals in Phyllotreta species (Coleoptera, Chrysomelidae): a minireview
93-100.Views:148Phyllotreta species (Coleoptera, Chrysomelidae, Halticinae) rank among the most important horticultural pests in the Northern Hemisphere. Leaf damage caused by flea beetles upsets the water balance, blocks plant growth and sometimes causes a high level of mortality of seedlings. Several species are known to act as vectors of numerous pathogens as well. Chemical communication plays an important role in the host finding, feeding and oviposition behaviour of flea beetles. In the first phase volatile mustard oils (isothiocyanates and thiocyanates) are released from the host plants through the decomposition of non-volatile glucosinolates (chemicals specific to Cruciferae) by myrosinase, and beetles are attracted to the source of release from a distance. Among the isothiocyanates, allyl isothiocyanate is the longest known and widespread compound utilized in the host-plant location of Phyllotreta species, but some species may have a stronger preference to other isothiocyanates or thiocyanates. The attractive effect of the plant volatiles is enhanced by the emission of a male-produced aggregation pheromone. The presence of such a pheromone was first demonstrated in Phyllotreta cruciferae Goeze. In this species R5R,5aS)-1,1,5,8-tetramethy1-1,2,3,4,5,6,5a-heptahydrobenzo[1,2-a][7]annulene] was found to be the main pheromone component. Significant attraction by the pheromone was recorded only in the presence of ALLYL ITCN. The biological activity of the pheromone compound was connected to the plus (+) chirality. The same component seems to be occurring also in the pheromones of several other Phyllotreta spp. as well, suggesting a wider occurence in the genus. Once attracted by the joint effect of plant volatiles and aggregation pheromone, the presence of the non-volatile glucosinolates in the plant tissues is necessary for continuous feeding. Aggregations of flea beetles on suitable host plants, which result from the joint effects of plant-derived and pheromonal chemical cues detailed above may also be good rendez-vous occasions, increasing the probability of encounters with the opposite sex and mating in the vicinity of the optimal oviposition site. Due to the horticultural importance of Phyllotreta spp., deciphering details of their chemical communication has considerable significance in the development of new methods of integrated control.
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Study on the time of emergence of the first generation of raspberry cane midge (Resseliella theobaldi BARNES)
43-45.Views:172The raspberry cane midge (Resseliella rheohaldi BARNES) is a major pest of raspberry in Europe. The accurate prediction of adult midge emergence is an important part of integrated raspberry protection. Calculation of the accumulated effective temperature may be used in prediction. The values of the critical accumulated effective temperature needed for the first flight of the midge differ in the European regions. In our experiments we investigated the first generation of the midge in Hungary. Our results show that the critical accumulated effective temperature for the first flight was the lowest compared with results received in other European countries. The emergence of males of the first generation was found at 145-194 day °C, and females started laying eggs a few days later.
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Floral analysis can be use as an early plant analytical tool to diagnose nutritional status of fruit trees?
23-27.Views:191A field experiment was conducted in an integrated apple orchard (Malus domestica Borkh.) established on a lowland chernozem soil in East-Hungary, to investigate if flower analysis could be used to diagnose the nutritional status of the trees. In April 2008, during full bloom, flowers and leaves were collected. Leaves were collected again in August, at the standard sampling time from the same trees. The content of nitrogen, phosphorous, potassium, calcium magnesium and boron were measured in flowers and in leaves. Correlation analyses were carried out to establish the relation s between nutrient contents in same and different plant part s. In flowers the following ratio was found between nutrients: N:P: K:Ca:Mg:B -10: l.35:7: 1.7: I :0,02. From result s it was evident that flower as organ contain significant and comparable amount of nutrients like as leaf. The N, K and Ca content were higher in leaves than in flowers at full bloom. The opposite was true for B. The P and Mg content of flower were quite equal with leaf P and Mg at full bloom . All examined nutrients were significantly affected by cultivars both in flowers and leaves. Significant correlation, was found between flower P and leaf P, flower B and leaf B (P=O.O 1) and flower K and leaf K at blooming time (P=0.05). Significant, but weaker correlation was also found between flower K and leaf K and between flower Mg and leaf Mg al JOO days after full bloom (P=0.05). Moreover, strong, but negative correlation was observed between flower B and leaf B at 100 clays after full bloom (P=O.O 1). Within flowers, the strongest positive correlation was found between Mg and B content (P=O.O 1). Strong positive correlations were also recognised between flower K and flower N, Ca and Mg and between N and Ca in the flowers (P=0.01). The strongest correlation was found between K and P in leaves at full bloom (P=O.O 1 ). Strong significant correlation was observed between N and B in the leaves collected at standard sampling time (100 DAFB) (P=0.01). Strong, but negative correlations were found bet ween leaf Mg and leaf P, K and between leaf N and leaf P at 100 days after full bloom (P=O.O 1).
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The new era of horticultural production after the millennium
87-90.Views:157The horticultural branches turn out, in relation to their area, a very great production. value. At the same time' many useful jobs are created. The development of gardening is, therefore, an important interest of our national economy. To the continuous enhancement of quality and quantity belongs the alloyage of new methods (biotechnology, integrated and organic procedures) with our traditional feats and with the incessant raise of the professional level.
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Preliminary data on attractiveness of phenylacetaldehyde-based lures on economically important plant bug pests (Hemiptera: Miridae)
87-94Views:280Several plant bug species (Miridae) are important pests of crops and vegetables, thus monitoring them is of essential importance for effective pest control. During the current, preliminary study synthetic plant volatile combinations were tested in field conditions in Hungary in alfalfa fields. Beside semiochemical baited traps, sweep-netting was also performed. In the experiments three plant bug species were found in higher numbers: Adelphocoris lineolatus, Lygus rugulipennis and L. pratensis. As a novel, interesting finding L. pratensis was attracted to phenylacetaldehyde baited traps. For all species, both males and females were trapped in all combinations. Sweep-netting and semiochemical baited traps showed different efficacy in case of the three species, as sweep-netting catches were highly biased for A. lineolatus, which indicates the higher efficacy of this method as compared to the tested semiochemical-baited traps. On the other hand, semiochemical baited showed better performance for L. rugulipennis and L. pratensis. For these species none of the tested combinations performed better than phenylacetaldehyde baited traps. The potential implication of results in view of monitoring are discussed.
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Effects of irregular cropping on nutrient uptake and status of apple trees
41-44.Views:188Produce of harmonic nutrient balance and status of trees is basic task of growers for qualified fruit growing. The role of crop loaded is essential to grow the productivity of trees without nutrient disorder or other nutritional problems. To reduce the frequency of biennial bearing there are some ways (site and species selection, crop regulation, irrigation, nutrition etc.) from them one is the best easy way the proper site and species oriented nutrition. Sometimes the responsible for irregular cropping is the insufficient fertilization. Observed irregular cropping at two apple cultivars (Malus domestica Borkh., cv. ‘Summerred’ and cv. ‘Mutsu’) in an integrated apple orchard at Hajdúnánás-Tedej, in 2007 resulted a chance to determine nutritional status of “bearing” and “non bearing” trees and investigate the effects of irregular cropping on nutrient uptake and possibilities of correction. Our results pointed out that irregularity is connection on nutrition status of trees. Irregular cropping results in insufficient nutrient uptake and disharmony of nutrients. For this reason, the establishment of nutrient status of “non bearing” trees is could permit a chance to correct and improve the nutrient supply of trees which help to breakdown the vicious circle of irregularity.
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Variety specific integrated fruit production development in order to optimize inner content values
27-31.Views:169In this paper we introduce our results of three years (2007-2009) investigatio ns carried out in the framework of "Research and development in foodstaff chain" - project of Regional University Knowledge Center by the members of Corvi nus U niversity of Budapest - Department of Pomology and. Research Institute for Fru it growing and Ornamentals. Budapest-Erd. The main objectives of the project were the followings: submi ssion of sour cherry candidate suitable for industrial process for state approval; determination of physica l parameter and inner content value changes of sour cherry varieties duri ng ripening; evaluation of health care attributes of sour cherry fruit; ripen ing process description by the colour and the force required to pick fruits of sour cherry. We stated that the optimal begi nning and period of the harvest can be determined wi th the fruit removal force. In this period the fruit growth stops. ju iciness rat io does not change. refractions are approximately equal.acidic content turns to decreasing trend.However the proportion of anthocyan in and polypheno l can still increase. Fruits harvested in this period fulfil a wide range of industrial process opportunities. 'Erdi jubileum ·and candidate IV-3/48 according to their inner content values arc suitable for high quality products (containing real fruit material in high proportion).
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Apple allergens as affected by cultivation technolog and variental factors
91-93.Views:211Our objective was to study the effect of organic farming and area of cultivation on the allergen content of apple varieties by quantification of the major allergens (Mal d 1 and Mal d 2) using allergen-specific ELISA. The obtained results showed that except Idared IX. 29. most of examined varieties, when cultivated under organic farming conditions, contained lower level of Mal d1 allergen than those produced with integrated farming. The different varieties were found to vary substantially in theirMal d 1 allergen content. Furthermore, with some varieties the area of cultivation affected to some extent the content of Mal d 1 allergen.