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Pollen tube growth in sweet cherry (Prunus avium L.) styles following fully compatible, half compatible and incompatible pollinations
63-68.Views:215In vivo as well as in vitro pollen tube growth studies along the style were performed, each with two pairs of sweet cherry cultivar combinations by means of fluorescence microscopy. In vivo studies showed that the percentage of pollen tubes penetrating the middle and basal section of the style was higher in the fully compatible 'Margit' x 'Alex' combination than in the half compatible `Germersdorfi 3' x `Alex' cross. The year effect was significant at P=0.] probability level. All pollen tubes in vitro stopped at the upper third of the style in the incompatible 'Vera' x 'Van' cross, whereas in the half compatible 'Alex' x 'Van' 50% of the pollen tubes penetrated to the lower third of the style. By in vitro fluorescence microscopy, it was possible to distinguish half compatible combinations from incompatible ones. Results obtained by in vivo technique only were much ambiguous.
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Inhibition and recovery of germination and growing ability of seedlings under and after osmotic stress induced by polyethylene glycol in 8 pea genotypes
53-59.Views:232Germination and early seedling growth of eight pea genotypes were examined under and soon after different (5%, 10%, 15%, and 20%) PEG treatments. Seeds were germinated on PEG solution for 3 or 6 days and then further germinated and cultured on filter paper moistened by water for 3 and 6 days. The length and fresh weight of shoots and roots of seedlings were measured and used for evaluation of genotypes. Roots were less inhibited by osmotic stress than shoots similarly to other plant species. The variability among the genotypes was the greatest in the case of shoot growth at 5% PEG treatment and in the case of root growth at 15% PEG treatment. Results suggest that growing responses of genotypes after cessation of stress are more suitable for the evaluation of their osmotic tolerance, than their responses expressed during in vitro stress conditions. Genotypes with relatively high or low osmotic stress tolerance, respectively, could be distinguished with 6 days after recovery from 3-day-long 15% PEG treatment concerning the rate of shoot weight to root weight.
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Development in intensive orchard systems of cherries in Hungary
76-86.Views:566High density central leader systems, the so called "spindle trees" are spreading in intensive stone fruit orchards established for hand picking in Hungary. Results of Brunner (1972, 1990) and Zahn (1967, 1996) inspired the researchers to implement their theories into practice under our climate and special soil conditions. For sweet cherry it is essential to apply an orchard system appropriate for hand picking because of the European market requirements. In intensive sweet cherry orchards two new training and orchard systems are developed and adapted to environmental conditions in Hungary based on previous inventions. The first step of the development is represented by modified Brunner-spindle, which applies the delayed heading of the central leader and the sectorial-double-pruning system from Brunner (1972), resulting intensive orchard of 600-800 trees/ha density, planted on standard vigour rootstocks. Modified Brunner-spindle trees are developed with a central leader and wide-angled branches on it. Light bearing wood is positioned on the central leader and wide-angled branches. During training, shoots for branches are bent or a sectorial double pruning is used. The growth of central leader is reduced by delayed heading, and the strong upright shoots are pinched in summer. Based upon tree size spacing of 5 m between row and 2.5-3 m between trees is recommended, tree height is around 3.5-4 m. This training system is useful for hand-picking; 60-70% of the crop can be harvested from ground. Modified Brunner-spindle is suitable for either standard or moderate vigorous rootstocks. The cherry spindle is an intensive orchard planted with 1250-2300 trees per hectare and it is recommended for sweet and sour cherries on semi dwarf to vigorous rootstocks, depending on soil fertility and quality. Trees are 2.5-3.5 m high, 75-80% of the crop can be harvested from the ground. Permanent basal scaffolds are developed on the basis of the canopy to counteract the stronger terminal growth. The tree is headed only once, after planting, from the following year the central leader grows from the terminal bud. The central leader developed from the terminal bud results moderated growth in the upper parts of the tree head. The strong upright shoots that may develop below the terminal bud are pinched to 3-4 leaves in the summer or removed entirely. The weaker, almost horizontal shoots growing from the central leader form fruiting twigs in the following year if their terminal bud is not removed. Brunner's double pruning is used only once or twice on the permanent basal branches because of its good branching effect. Trials on various rootstocks are running to find optimum spacing and fruiting wood management. The training and pruning guidelines are discussed in the paper. The average crop of bearing years is around 20-30 t/ha depending on site and cultivars. This new system is spreading in Hungary, around 70 ha sweet and sour cherry orchards are trained according to our guidelines.
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Rootstock effects on fruit drop and quality of 'Arlet' apples
69-75.Views:716The aim of this study is to investigate the effect of different growth inducing rootstocks on fruit drop of apple. This research was conducted for 3 years at Nagykutas, Western Hungary on apple cv. 'Arlet'. For the experiments, 3 different growth inducing rootstocks were choosen: M.9 (weak), MM.106 (moderate) and crabapple seedling (strong). There were 3 fruit shedding periods on the trees grafted M.9 and MM.106 rootstocks, but in the case of crabapple seedling, only 2 were found. The measure of fruit drop was closely related to seed count of fruits; seed number was the lowest, fruit drop was the highest. The lowest seed number was counted in fruits from trees on crabapple seedling. Seasonal changes of leaf:fruit ratio mainly depended on shoot growth and fruit drop. The rise of the curve of leaf:fruit ratio was very important during the first phase of fruit development, in especially at the end of June and in the beginning of July. The cause of this is that first and second periods of fruit drop appeared during this term. The rise of the curve was important in the beginning of June and the end of July on crabapple seedling. Decreasing tendency of quality parameters was found of fruits from trees on M.9, MM.106 and seedling rootstocks, except flesh firmness which was the highest in fruits from trees on crabapple seedling.
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Relations of phenometrical indices of apple fruits with weather variation in the assortment of varieties of an apple gene bank
115-120.Views:625The purpose of phenometry is to examine the measurable parameters of the plants in order to follow up the consequences of weather processes. We should fi nd the reasons, why the diameter of fruits grows larger in one season and smaller in the next. Variation may occur as a response to insuffi cient provision of water or nutrients, but also because of pathological effects and of extremely high or low temperatures, moreover, of extraordinary heavy fruit load. There are phenometrical characteristics, which consider the fi nal consequences (density of fl owers, fruits set, drop of fruits), whereas other parameters could be followed up (size, length and width of fruits) as the dynamic components of growth. The quantitative parameters of growth are functionally related to each other, where the weather conditions, soil humidity and nutrients are on the input side, thus it is possible to model the growth of fruits as a function of the environment. Initially, the relations between the main weather variables and the phenometrical data have to be cleared. In the present study, the interactions between the mentioned phenomena are presented and numerically defi ned.
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Treatments for improving tree growth, yield and fruit quality and for reducing double fruit and deep suture incidence in “Desert red” peach trees
7-19.Views:478Five years old “Desert red” peach trees budded on Nemaguard rootstock and grown in sandy soil at commercial orchard Al-Nubaria city, El-Behira Governorate-Egypt were treated with some agricultural treatments involving thinning out pruning, fruit thinning and foliar application of potassium silicate (25% S +10% K2O) at 0.1% and super grow (20% N, 20% P2O5 and 20% K2O) at 0.3% in 2014 and 2015 seasons, to study their effects on yield and fruit quality and the relationship between nutrient balance and yield of “Desert red” peach trees. Beside, testing the influence of used treatments on two physiological disorder, double fruit and deep suture %. Also, economic evaluation of different treatments was done. All obtained data were statistically analyzed using a randomized complete block design. Depending on the obtained results in this study, it could be concluded that application of thinning out pruning 35%, fruit thinning by leaving 15 cm between fruits on one-year old shoot at 20 days after full bloom and foliar application of potassium silicate which sprayed five times during each growing season at fruit set, the second fruit development stage, the beginning of the fruit color change and twice after month from harvest, most profitable treatment for peach trees grown under conditions of this investigation. This treatment gave the best vegetative growth, yield, fruit quality, higher crop value with high net income /fed. from “Desert red” peach trees, in addition, reduced the percentage of double fruit and deep suture by more than 50% in both seasons, therefore, the study recommends this treatment for “Desert red” peach growers.
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Rootstock evaluation in intensive sweet cherry (Prunus avium L.) orchard
7-12.Views:364During 2000 and 2007, rootstocks of different vigor have been tested in a high density sweet cherry orchard with 'Vera '® and 'Axel'® cultivars at 4 x 2 meter row and plant distance. Trees are trained to Hungarian Spindle with permanent basal branches; in the alley way naturally grown grass is managed by mowing. The first considerable fruiting was in 2004. Every year we measured trunk and canopy parameters of the trees, productivity and fruit size. Our conclusion is that the rootstocks considerably affected the growth, precocity, as well as tree and orchard productivity, fruit weight of sweet cherry cultivars, but these rootstock effects are modified by cultivars, except for growth vigor. According to our results Cema, SL 64, and Bogdany are vigorous rootstocks, moderate vigorous are MaxMa 97, Pi-Ku I , and Tabel® Edabriz, Gisela® 5 and Prob are dwarfing rootstocks. Besides the precocious Gisela® 5 also mahaleb rootstocks CEMA, Bogdany and SL 64 showed considerable precocity, which can be explained by the larger bearing surface to the time of turning to bearing, and a similar or relative large density of burse shoots on fruiting branches. Cumulative yield of 'Axel'® was the highest on Bogdany and on Cerna, contrary to Gisela® 5, which produced only 50% of the previous ones. Cumulative yield of 'Vera'® was the highest on SL 64, and no significant difference was found, compared to trees on rootstocks Cema, Bogdany and Pi-Ku I . Cumulative yield production of trees was smaller on Gisela® 5, Prob, Max Ma 97 and Tabel® Edabriz rootstocks. Corresponding to the literature data of yield efficiency calculated on TCSA basis was highest on Gisela® 5 rootstock. but the efficiency calculated on canopy volume of 'Axel•® trees was similarly high on CEMA and Bogdany, and that of 'Vera'® trees relatively high on CEMA, Bogdany, SL 64 and PiKu I rootstocks. When calculating orchard efficiency al spacing 4 x 2 meters (1250 tree/ha), we received highest yield values on Bogdany, CEMA, SL 64, and PiKu I rootstocks, with large fruit weight. Rootstocks also affect fruit weight. We measured the largest fruit weight on trees on Bogdany.
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Evaluation study for the effect of four mango rootstocks on behaviour of Keit mango variety
49-53.Views:402Our investigation was carried out in four mango rootstocks were namely Zebda, Socaria, 13/1 an 4/9. This study reviewed the effect of these rootstocks on growth behaviour of Keit mango variety as a scion grown on them. This evaluation and comparison included data on following aspects: the vegetative growth characters, root growth parameter, uptake of some macro nutrient and some organic substance. All results in this regard refer that the Socaria rootstock gave the highest values off all parameters so it is considered as a vigorous rootstocks, means that the trees on this rootstocks will reach to high size after short time. By contrast, Zebda rootstocks gave the lowest values in this concern and this will reflect on tree size on it. This means that the Zebda rootstocks will be useful in the system of high density orchard comparing with Socaria. Concerning to other rootstocks, they were intermediate between Socaria and Zebda rootstocks.
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Genotypic difference of garlic (Allium sativum L.) cultivars for growth, bulb yield and yield related attributes at Tigray Province, Northern Ethiopia
46-52.Views:305The experiment was conducted at Ofla district from June to October of 2017 and 2018 cropping season to select high yielding and adaptable garlic cultivar(s). Six garlic cultivars namely Chefe, Tsedey, Holeta local, Kuriftu and Bora-4 and one Ofla Local were evaluated. The experiment was arranged in completely randomized block design (RCBD) with three replications. Different growth, phenology, yield and yield related data were collected. Leaf length, leaf width, bulb diameter and length were significantly (p<0.05) influenced by garlic cultivars in both cropping seasons. Bulb weight and number of cloves per bulb were highly significantly (P<0.01) influenced by cultivars in both cropping seasons. Also, marketable yield, unmarketable yield and total yield ha-1 were highly significantly (P<0.01) influenced by cultivars in both seasons. During 2017 and 2018, the highest marketable bulb yield was observed from Ofla Local cultivar with an average value of (8.86 t/ha) and (9.06 t/ha) respectively. During the 2017 cropping year, the maximum and significantly higher total bulb yield of 10.21 t/ha was recorded on the Ofla Local cultivar. Moreover, in 2018, this cultivar provided the maximum total bulb yield of 9.80 t/ha. Therefore, the Ofla Local cultivar showed the greatest performance for growth, yield and yield related attributes. Thus, it is recommended for cultivation in areas having similar agro-ecology. To improve the productivity of garlic, it is important to study and identify the optimum fertilizer level and spacing in the study area.
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Tending operation models for black locust (Robinia pseudoacacia L.) stands growing on sandy soils in Hungary
55-57.Views:347A more intensive integrated research and development approach to the work carried out on the growth on sandy soils of stands of black locust (Robinia pseudoacacia L.) has been adopted in recent years, revealing several factors influencing stand growth. The fact that certain ecological factors influencing fundamentally the growth of trees have become unfavourable in Hungary in recent years has led to the more extensive use of black locust in the course of afforestation and forest regeneration schemes. The study presents a new, simplified tending operation model for black locust stands and age, growing space and target diameter models suitable for qualitaty log production and for mass assortments. The simplicity of these practice-oriented
models may foster the qualitative development of black locust management in Hungary and in some other countries where this tree species may gain greater acceptance by landowners and the forest industry. -
Effects of different levels of NaCl and CaCl2 on seed germination characteristics of Melissa officinalis L. and Ocimum basilicum L.
21-25.Views:465More than 15 million hectares of Iran’s land areas are suffering from salinity.We investigated the effects of two types of salt (NaCl, CaCl2) in different levels on the germination and initial growth of two medicinal and aromatic plants, Melissa officinalis L. and Ocimum basilicum L. The experiment was carried out in Qazvin Agriculture and Natural Resource Research Center based on a randomized complete block design, for 14 days in a germinator. Sweet basil germinated above 50% in each saline treatment (EC=10 dSm-1), in alkali treatment (CaCl2=18800 mg/L) and in sodic-saline treatment (Ec=10 dSm-1; SAR=5.6), however at the highest concentrations germination was slowed down. Lemon balm showed a higher sensitivity: except the lowest NaCl treatment, each other ones inhibited germination and its rate to some extent. The combined treatments of both salts increased the stress reactions. However, seedling growth was less effected. The results showed a stimulating effect of NaCl treatment in 2500 mg/L (EC<5 dSm-1) for both species concerning germination and seedling growth.
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Knot formation by Pseudomonas syringae subsp. savastanoi on the in vitro shoots of Sorbus redliana
59-62.Views:370Two strains of Pseudomonas syringae subsp. savastanoi were isolated from Forsythia sp. and Nerium oleander in Hungary in 1997. The effects of growth regulators produced by the bacteria were studied in different experiments. The strains were co-cultured with Sorbus redliana in vitro shoots without being in contact with the plant on solid media. Further culture filtrates in different concentrations were added to the culture medium. The growth regulators presented in the agar caused knot formation on the shoots and on the leaves in both kinds of culture. There were significant differences in the cultural and physiological characters, auxin and cytokinin activity of the strains of different origin.
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Vegetative Indicators of sour cherry cultivars and resistant clons
55-58.Views:300Sour cherry is a hungaricum. Hungary has several excellent and self fertile sour cherry cultivars. Hungarian sour cherries have a name for fruit quality and they are suitable for fresh consumption. Today breeding is focused not only on fruit quality, but also on disease resistance. The new, investigated cultivars have these characteristics too. But growth characteristics are also very important because they contribute to successful production. The vegetative and generative properties of the cultivars are different. If a cultivar is very productive, the ignorance of cultivar-specific pruning may result in the decrease in tree condition and fruit quality. The properties of producing fruiting wood greatly determine the renewal capabilities of cultivars. Knowing these properties is essential in case of sour cherries, the fruit species that requires a lot of sunlight and tends to produce bare wood. The research of candidate cultivars until now focused on fruit quality. Our aim was to investigate the vegetative characteristics of the resistant ‘bosnyák’ cultivar group in detail, defining the growth characteristics of these cultivars.
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The effects of growth regulators in proliferation of Sorbus redliana 'Burokvölgy'
77-83.Views:326The Hungarian cultivar Sorbus redliana 'Burokvölgy' was proliferated on Murashige and Skoog (MS, 1962) medium with half-strength macroelements and 100 mg/1 meso-inositol, 20 g/1 sucrose, 11 g/1 agar-agar. Different combinations of kinetin (KIN), metatopolin (mT), benzyladenine (BA), benzyladenine-ribosid (BAR) and indolebutiric acid (IBA) were tested, and pH was adjusted to 5.6 every case using KOH. The cultures were incubated at 20-24 °C in 8/16 hours dark/light photoperiod for 50-52 days. The main aim of our research was to find the optimal growth regulator and its optimum concentration. Purthermore, to determine the chlorophyll contents of the in vitro propagated plants' leaves. During the proliferation, the highest number of shoots were observed in the case of using BA + IBA, and on the medium containing 0.75 mg/I BA + 0.05 mg/1 IBA 8.93 shoots were found. The addition of KIN + IBA decreased the number of shoots and increased the sizes of leaves — the widest (11.2 mm) and longest (17.8 mm) leaves were obtained on the medium containing 1.00 mg/I KIN + 0.05 mg/1 IBA. The longest shoots (36.46 mm) were found in the case of applying 0.75 mg/1 BAR + 0.05 mg/I IBA. The BA + KIN + IBA combination resulted the shortest shoots. Sometimes not only shoot regeneration but spontaneous rooting was observed during the multiplication. The highest chlorophyll content (1.569 mg/g total chlorophyll, 1.132 mg/g chlorophyll-a, 0.437 mg/g chlorophyll-b) was obtained in the presence of 1.0 mg/I KIN + 0.05 mg/1 IBA.
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In vitro investigation of King Oyster Mushroom [Pleurotus eryngii (DC.: Fr.) Quel.] strains in vegetative growing phases
47-53.Views:324The king oyster mushroom ( Pleurotus eryngii) is more and more popular amongst the producers due to its excellent taste and relatively easy cultivation . In the course of our work we collected 15 king oyster mushroom strains from various habitats in Hungary in order to get a better picture about the growth of the vegetative mycelia of the species and its different strains. In the in vitro experiments we investigated the growth of the strains at various temperatures and pH. incubated them in light and darkness and in aerobic and anaerobic atmosphere. In addition to these we measured the weight of dry mycelia produced in a given time by the strains. Our results showed that the above mentioned environmental conditions resulted in a very different growth rate of the vegetative mycelia of the various P. eryngii strains. These results may provide valuable data about the vegetative phase of the cultivation.
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Growth and productivity of a young apple orchard at different spacing
23-27.Views:284Planting of new high density apple orchards showed an increasing tendency over the last ten years. Growers use in those orchards mainly dwarfing or semi dwarfing rootstocks. The spacing for those orchards is recommended based on Dutch and German experiences; however, the optimization of orchard planting density as a key factor for successful orchard management should consider the local climate conditions. An experimental orchard was planted in 2000 to investigate the effect of spacing on three dwarfing rootstocks with two apple cultivars `Jonica' and 'Gala Must'. We compared 8 planting densities (1270-3704 tree/ha) and two tree shapes (slender spindle and vertical axis). In this paper the data of the first five years' growth and bearing are presented. After 4 years, the decreasing tree densities caused reduced trunk cross sectional area. Tree density had a significant positive effect on cumulative yield per hectare. From the examined rootstocks, M.9 Burgmer 984 gave the smallest canopy for both cultivars. The trees on M.9 T.337 and on Jork 9 rootstocks have stronger growth. The light interception was measured under the canopy by AccuPAR (Decagon Devices Inc.'s).
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Impact of different growing media on propagation of bougainvillea (Bougainvillea spp.) through hardwood stem cuttings in Chitwan, Nepal
37-43.Views:520An experiment was conducted in Rampur, Chitwan, Nepal, in a Randomized Complete Block Design (RCBD) with five treatments: T1 (sand:soil, 1:1) [control], T2 (sand:cocopeat:perlite, 1:1:1), T3 (perlite:sand, 1:1), T4 (sand:cocopeat, 1:1), and T5 (peat moss:perlite, 1:1). Each treatment was replicated four times with a total of 300 planted cuttings. The key parameters evaluated were sprouting percentage, shoot growth (length, leaf number, and branch number), and root development (length, number, fresh, and dry biomass). Analysis of data was done using R-Studio, and Duncan’s Multiple Range Test (DMRT) was performed at a 5% significance level. The results revealed that peat moss:perlite (1:1) demonstrated the highest shoot growth with shoot length of 6.00, 14.38 and 28.78 cm at 30, 60, and 90 Days After Planting (DAP) respectively. Leaf number were 6.25, 13.12, and 19.57, while branch numbers were 1.76, 2.00, and 2.18 at the same intervals., Additionally, the fresh root weight was recorded 8.74 g. Sand:cocopeat:perlite (1:1:1) recorded the highest sprouting percentage (81.66%) and root length (17.11 cm), while sand:cocopeat (1:1) achieved the highest root number (18.37) and dry root weight (0.99 g). Perlite:sand (1:1) exhibited moderate performance with sprouting percentage 69.99%, and root length 12.56 cm. In contrast, control (sand:soil) showed the least favorable results with the lowest sprouting percentage (58.33%), the fewest number of roots (7.81), and the minimum root length (9.73). This study concludes that peat moss:perlite (1:1) is the most effective growing medium for bougainvillea propagation, offering a practical solution to enhance rooting success and growth performance. Sand:cocopeat (1:1) with maximum dry weight of root and maximum number of roots emerged as a reliable alternative, proving the effectiveness of the medium.
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Allelopathic effect of the Cladonia verticillaris lichen extracts and fumarprotocetraric acid on the early growth of germinating seeds and seedlings in Allium cepa L.
31-35.Views:242The allelopathic activity of the different extracts of the lichen Cladonia verticillaris and fumarprotocetraric acid on the early growth of A cepa (IPA 6) seedlings depends on their chemical composition and concentration, respectively. It was observed that the length of the radicle was significantly stimulated by fumarprotocetraric acid at high concentrations and by the total extract of C. verticillaris thalli, which contained a high level of fumarprotocetraric, acid confirmed by HPLC-technique. In addition, it was found, that the phosphate buffer extract, which contained high level of methyl (3-orcinol carboxilate measured by HPLC, reduced the length of the hypocotyls significantly. Under our experimental conditions there was no influence of the different types of extract and fumarprotocetraric acid on the ratio of seed germination, in relation to the control. From the study of HPLC it was found that fumarprotocetraric acid and methyl 13-orcinol carboxilate were present in all extracts at different concentrations, depending on the method of extraction.
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Rootstocks for Cherries from the Department of Fruit Science Budapest
63-66.Views:282Cherry rootstock breeding started at the Department of Fruit Science, SSU Budapest by the late 50-s and the activity can be divided into three main groups. In the first stage the activity was focused on collection of native mahaleb cherry (Prunus mahaleb L.) varieties lead by L. Sebők. After evaluation in the nursery and orchard tests there are four promising rootstock cultivars selected from this material: 'Korponay' used as self fertile seed tree, its seedlings are recommended for sour cherries. The mahaleb varieties 'Bogdány' (vigorous), 'Egervár' and 'Magyar' (medium vigour) are propagated by cuttings. The next project has started in 1979 with the aim to select self fertile mahaleb seed trees producing homogeneous seedling populations with reduced vigour. Inbred populations from isolated flowering self fertile trees were produced and planted out in 1980. The inbreeding of 'Korponay' self fertile P. mahaleb variety resulted in specimens with different fruit colour (yellow, red, black), fruit shape and size. From among them self fertile trees were selected with various growth characteristics. Seedlings of that self fertile mother trees (S2 population) were tested in seedbed, they showed homogeneous phenotype characteristics as liners in the nursery. As rootstock of 'Érdi bőtermő' sour cherry in the orchard most of the S2 lines proved to be less vigorous in comparison to SI popuplations. 'Érdi bőtermő' trees budded on certain S2 lines in the orchard are more productive than those on S1 ('Korponay' seedling). Characteristics of the S2 generation as seed tree were studied as well. We expect to get morphologically homogeneous seedling populations with different growth vigour and good productivity in the later inbred generations. In the last couple of decades the research activity concerning ground-cherry and its hybrids resulted in dwarfing rootstocks. Prunus fruticosa Pall. hybrids from the natural flora of Hungary were collected and artificial hybrids were created between P. fruticosa and mahaleb cherry. Most of them are in the initial tests, only one of them is before registration, named 'Prob', which is a dwarf rootstock for sweet cherry. By the screening of new hybrids medium vigorous or semi dwarfing and precocious rootstocks seem to be promising for the cherry industry.
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Vegetative and micropropagation potential of Piper guineense (Schumach and Thonn)
29-36Views:322The continuous loss of forest plants due to deforestation, and the increasing demand for Piper guineense because of its medicinal and food value, has put a permanent pressure on its population in the wild where it is collected. A method for conservation and mass propagation is therefore required. This research was undertaken to determine the optimal concentration of auxin needed for vegetative propagation and to investigate the potential of Piper guineense for micropropagation. The auxin optimization study of vegetative propagation was based on the use of two-nodal stem cuttings treated with five different concentrations of indole-butyric acid (IBA). Growth parameters such as the number of sprouted, rooted and survived cuttings among others were determined. To investigate the potential of Piper guineense for micropropagation, nodal explants were subjected to different sterilizing treatments using ethanol, NaOCl, mancozeb, streptomycin and Plant Preservative Mixture (PPM). The effect of plant growth regulators (PGRs) was tested on sterilized nodal explants using full strength Murashige and Skoog (MS) hormone-free media alone as control and MS media supplemented with PGRs (BA, NAA and KIN) at different concentrations and combinations. Significant differences were observed across the treatments for all growth parameters measured. However, 2000 ppm IBA significantly (p<0.05) influenced sprouting and rooting of the stem cuttings. Piper guineense explants have deep tissue contaminants, which cannot be eradicated by surface sterilization alone except double sterilization using PPM. On control media, neither shoot nor root response was observed while the highest percentage of induced roots was obtained from explants cultured on MS +1 mg/L BA + 0.25 mg/L NAA. Shoot induction was only achieved when BA was used alone and when subcultured on media supplemented with NAA, which generated roots.
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Effects of crop load on tree water use in apple (Malus x domestica Borkh.)
29-31.Views:238Sap flow rate measurements were carried out during two consecutive ('on' and 'off') years in an apple orchard cv. 'Florina' M.26 to analyse the effects of various crop load on tree water use. Sap flow rate was measured by thermal dissipation method in trunks of nine trees from June to the harvest. Crop load was between 0.2-9.0 fruits • cm -2 of trunk cross section area (equal to 0.5-35.0 fruits•m-2 of leaf area), trunk diameter varied between 4.7-8.7 cm.
Total leaf area was estimated by leaf counting or using shoot girth and leaf area relationship. In both years, fruit growth rate was determined by measuring diameter of 280 marked fruits with 7 days frequency. Fruit volume calculated as a function of fruit diameter based on a previously determined relationship.
Total leaf area of trees ranged from 4.4 to 19.5 m2 and it was closely related to trunk cross section area. At high crop load the fruit growth rate peaked in August with 0.51.tree-l.day-1 and the leaf area specific total fruit volume reached 61 m2 before the harvest.
There was a linear relationship between total leaf area and daily water use, while the leaf area specific water use was influenced by crop load rate. The relationship was described by piecewise linear regression with the breakpoint at crop load rate of 12 fruits•m2 of leaf area. At low crop load the slope of the fitted regression line was less than at high crop load rate.
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Effect of hail protecting nets on the quality of apples
77-80.Views:755Today, successful fruit growing depends largely on the security of production. Among the technological elements of a secure growing system, the use of hail protection nets serves special attention. Components of security are the regularity, conspicuous large yields with excellent quality, which determine the prices and profitability as well (Szabó et al., 2010). In the European Union, most of the apple growing countries produce higher yields by 10-20% than Hungary. The conditions of better yields are partly due to a more favourable ecological milieu, partly to more developed technologies and serious discipline of the operations. Our own competitiveness could be developed first of all in those moments. As a consequence of global climatic changes, excesses of weather, i.e. hail-storms became more frequent. Different methods of protection against hail are developed abroad but also in Hungary. The examination of effects of the hail protection nets compared with the check without nets has been the purpose of our experiments. The growing system was the intensive one with high planting densities. Fruits of the two subsequent years have been compared as grown with and without hail protective nets. It turned out that in one season when shoot growth was stimulated, the shadowing effect of the net increased in addition the growth and at the same time braked the differentiation of flower buds, which exerted negative effects on yield of the next year. Let alone this phenomenon, the quality of fruits was not influenced by the hail protection net.
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The effect of Regalis® (prohexadione calcium) on the reduction of fire blight (Erwinia amylovora) severity in apple trees
61-66.Views:324Due to the lack of effective and non-phytotoxic materials for control of the blossom and shoot blight phase of fire blight in pome fruit trees, two novel control strategies have emerged: shoot growth retardation by bioregulators and applying resistance inducer compounds. Prohexadione calcium (ProCa) is the active ingredient of the bioregulator Regalis® registered in several European countries. The reduction of shoot growth elongation is the most obvious effect of ProCa. Furthermore, it causes significant changes in the spectrum of flavonoids and their phenolic precursors, leading to the considerable reduction of susceptibility to fire blight.
In Poland, potted one-year-old apple trees of cvs. Gala Must grafted on M.26 and Sampion on M.9 (in 2001) as well as Gala Must on P.60 (in 2002) were treated with Regalis® at a range of concentration of 250, 150 or 150 + 100 ppm, respectively. The inoculation of shoots was made with the strain No.691 of E. amylovora (107 cfu/ml), on the 7th and 21st'day after treatments with Regalis. In Hungary, during the years of 2002 and 2003 one-year-old container grown apple trees of the cvs. Idared/M.9 and Freedom/M.9 were treated with the prohexadione-Ca, the active ingredient of Regalis® 100, 150 or 200 ppm, two weeks before inoculation with the Ea 1 strain of E. amylovora (107 cfu/m1).
In Poland, the suppression of fire blight in shoots reached up to 80%, depending on concentration and application time of Regalis®. In Hungary, the effect of prohexadione-Ca treatments, determined by the length of necrotic lesion developed, proved to be better than that of streptomycin used for comparison.
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Effects of different rootstocks on the morphologic parameters of watermelon grafts during transplant production
69-73.Views:318Before Hungary joined the EU watermelon production was a key sector of the Hungarian vegetable industry. Its production area shrank by nearly 60 % since 2004, it is only 5000 ha today. Stable and reliable market failed to evolve in the last years and extreme weather events occurred more frequently. An unfavourable growing season can notably affect the production volume of the next season. Information gathered in the last years support the assumption, that suitable stocks can help eliminate the issues caused by extreme weather or short-term crop rotation. Choosing the right grafted or non-grafted transplant is not enough any more, growers also have to select stock. In fruit and vine production choosing the stock, which is the most suitable for the technology is evident and this approach should be widely adapted by watermelon growers as well. In this research we investigated the growth parameters of different watermelon stocks and their effect on the scion during the transplant production. Measurements were carried out on seven different stock-scion combinations of the scion ’Topgun F1’ hybrid. Two of the stocks were interspecific (Cucurbita maxima × Cucurbita moschata) and five stocks were Lagenaria-type (Lagenaria siceraria) stocks. We made and recorded observations of the transplants’ length (cm), diameter of the stock and scion (mm), number of leaves (piece), root length (cm), root mass (g) and shoot mass (g). We concluded that early pricking out of transplants with interspecific stocks is successful due to their hardiness and vigorous growth. The effect of the ’Shintosa camelforce F1’ interspecific stock was outstanding. Furthermore, the results confirmed the practical observation that the root system of interspecific stocks grow vertically more allowing them to take up water and nutrients from lower soil layers in poor sandy soils. From the Lagenaria group the root system of the ’DG-01 F1’ was similar to the interspecific stocks’. It can be grown even in sandy soil, however other Lagenaria-type stocks are more suitable for production in hard soils with nutrient supply because of their compact root system. This study can serve as a basis for further research in the topic that can conduce to site- and technology-specific stock selection.
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Analyses of the pathogen and weather components of disease progress for modeling apple scab epidemics in integrated and organic production systems
101-106.Views:452The pathogen and weather components of apple scab disease progress were analysed in a three-year study, in two environmental-friendly production systems (organic and integrated) on cvs. `Idared', `Jonica' and 'Mutsu'. Linear regression analyses of transformed disease incidence and severity data and "area under the disease progress curves" (AUDPC) were used for the analysis of the pathogen component. To evaluate the role of the weather component in apple scab epidemic, first, the weekly disease increase was determined at a certain week (n). Weekly disease increase was related to rainfall, relative humidity, Mills' wetness period, temperature and interaction between temperature and relative humidity. Five different periods were used in the analyses: i) week (n-1), ii) week n(n-1), iii) week (n-2), iv) week (n-1)(n-2) and v) week n(n-1)(n-2). In the analyses of the pathogen component, the best transformation function was the logistic one. Regression analyses showed that disease growth rates were higher for disease incidence and for the organic production system than for disease severity and for the integrated production system, respectively. Disease growth rates for leaf incidence were higher than fruit incidence on all the three cultivars. AUDPC values showed great differences in both leaf and fruit incidences among cultivars and between the two production systems. The results the of analyses of the weather component showed that the best relationships between disease increase and weather parameters were found for fruit incidence and leaf incidence in week (n-2) in the organic and integrated production systems, respectively. Results also demonstrated that in week n(n-1) temperature played a more important role in the fungus development than the water parameters (relative humidity, rainfall and leaf wetness). Consequently, infection process is significantly dependent on almost all weather parameters, but during the incubation period the most important weather parameter is the temperature. Results were compared with similar studies and biological interpretations of the analyses are discussed.
