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The effect of different herbicide on the number and activity of living microorganisms in soil
76-82Views:136Sustainable plant growth, considering the difficulties of weed elimination, cannot be effective without the application of herbicides. However, these chemicals have enormous ecological implications, including effects on the microbiological communities of soils. It is advisable to use herbicides that have minimal secondary effects on the environment and soil-living microorganisms. In contrast, herbicides with prolonged growth stimulating or inhibiting effects are not suitable, because both types have strong influences on the number and activity of bacteria, thus causing changes in the ecological equilibrium.
Preceding small plot experiments, laboratory tests were carried out to study the effect of herbicides used in maize cultures on the number of bacteria and growth of microscopic fungi.
Substances that were observed to have stronger influences were applied in small plot experiments set up in the experimental garden of the Department of Plant Protection of the University of Debrecen. We studied the effects of four herbicides (Acenit A88EC, Frontier 900 EC, Merlin SC and Wing EC) on the microbiological properties of the soil. These herbicides were used in different concentrations in maize culture, and we investigated the effects in different soil layers.
In the laboratory experiments, we determined the total number of bacteria and microscopic fungi and examined the growth of Aspergillus niger, Trichoderma sp. and Fusarium oxysporum on peptone-glucose agar containing herbicides.
During the small plot experiments, soil samples were collected 3 times a year from 2-20 cm depth. The total numbers of bacteria and microscopic fungi were determined by plate dilution method, while the method of most probable number (Pochon method) was used to determine the numbers of nitrifying bacteria and cellulose decomposing bacteria. To evaluate the microbiological activity of the soil samples we measured carbon-dioxide release (after 10 days incubation), nitrate production (after 14 days incubation) and the concentration of C and N in the biomass.
We can summarize our results as follows:
• In laboratory experiments, herbicides caused a decrease in the number of bacteria and inhibited the growth of microscopic fungi.
• Frontier 900 EC and Acenit A 880 EC had the strongest inhibiting effect on microorganisms.
• In small plot experiments, herbicide treatment decreased the total number of bacteria and microscopic fungi.
• Herbicides caused a significant increase in the number of nitrifying and cellulose decomposing bacteria.
• Different herbicides containing the same active compound had similar influences on soil microoorganisms.
• A significant increase was observed in the physiological processes of tolerant microorganisms surviving the effects of herbicides -
Microbiological and Chemical Characterization of Different Composts
106-111Views:80Composting of agricultural waste is considered particularly important from the point-of-view of environmental protection. Degradation of organic substance results in a significant reduction of waste volume.
The end product of the composting process, mature compost, can be used as soil coverage against excess loss of wastes, for mulching, for organic manure etc. The problem of composting has come into limelight in environmental studies and in agriculture.
The quality of the mature compost is determined by physical, chemical and biological parameters of the composting process which, in turn, depend on initial composition of the raw materials, the technology, e.g. regular mixing and moistening and on environmental factors. Quality is the key question in compost use.
We studied the composting process in compost windrows of different raw material composition. We measured temperature, humidity content, pH, organic substance content, nitrogen and carbon content.
We counted the number of bacteria, microscopic fungy, ammonifying and cellulose decomposing microorganisms. We directed the composting process with turning weekly (to provide oxygen) and watering (to provide humidity content 40-60%).
We set up windrows of 1 m3 volume from dry plant substances (cornstalk, pea straw, tomato stalk and crop, weeds) and cow manure not older than 1 week. The cow manure was used at ratios of 0%, 35%, 50%, 65% and 100%, respectively.
We measured changes in compost temperature relationship with outside temperature until they were almoust the same. Humidity was 40-60% in most cases.
At the beginning of the process, pH was slightly acidic-neutral; it later becomes neutral-slightly alkaline (pH: 6.93-8.02) as ammonia is liberated from proteins.
At the end of the process, pH decreased again, due to humification.
Organic substance content decreased as microorganisms mineralized them. Organic carbon content decreased gradually due to microorganisms used it as an energy.
Total nitrogen content increased until middle of july and decreased gradually until than.
The carbon/nitrogen rate were higher in the beginning, it decreased until july-august and increased by smaller degree until end of the process.
The number of bacteria was higher in the first three weeks and between june-september. The number of cellulose degrading bacteria was the highest in the first three month, the number of ammonifying bacteria was the highest from the end of may until sepember.
The number of microscopic fungy was significant in the second part of process, after july. -
Study of the microbiological activity in the agrocenosis from Crisurilor Plain
73-77Views:125The seasonal variations of soil microorganisms depend on changes in the soil chemical properties and the microbiological processes of soil are determined in main by different cropping systems, soil management and season. Investigation of the microbiological properties of a haplic luvisol, under different cultivation conditions showed that anthropogenic factors such as fertilization and treatments with pesticides was favourable for certain microorganisms while others were inhibited by these factors. In order the quantitative occurence of microorganism was aerobic mesophilic heterotrophs (105–107 cellsxg-1 dry matter soil) were followed by Actinomycetes, yeast and mould (103–106 cellsxg-1 dry matter soil), nitrogen fixing bacteria (102–105 cellsxg-1 dry matter soil) and nitrifying bacteria (10-1–103 cellsxg-1 dry matter soil). The highest values of aerobic mesophilic heterotrophs were found in cropland and undisturbed meadow of haplic luvisol. Actinomycetes are more developed in undisturbed soils than in the cultivated soils. The number of yeast and mould was high in the apricot tree orchard, and Azotobacter and nitrifying bacteria were identified in a small number in all the soil variants studied. Among the total number of aerobic microorganisms, pH and humus content statistically proved relationship was established. Total number of yeast and mould depends on the proportion of 10.89% by moisture content and pH. The numerical presence of Azotobacter depends in a proportion of 9.6% by the ammonia nitrogen content and pH variations. The numerical presence of nitrifying bacteria depends in a proportion of 1.69% by the nitric nitrogen content and humus content variation.
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Examination the effects of different herbicides on the soil microorganisms of a calcareous chernozem
121-126Views:91Pesticides play a key role in fighting weeds, pests and parasitic fungi. According to surveys, pests reduce the yield of agricultural crops by 35% worldwide. Pests, fungi and weeds account for 14%, 12% and 9% yield loss, respectively (Gáborjányi et al., 1995). Chemicals have contributed to increasing and maintaining the yields of crop production for decades. Today, agricultural production (in spite of many efforts) is unthinkable without the use of pesticides (herbicides, insecticides and fungicides). On the other hand, these chemicals contribute to the pollution of the atmosphere, surface and underground waters, and agricultural soils, especially if they are applied improperly.
The sustainable agricultural production pays attention to environment-friendly cultivation-technologies; but at the same time it makes an effort to produce good quality and economical products. The examination of the herbicides’ secondary effects, fits into this chain of idas namely, how the herbicides affect – stimulating or inhibiting – the soil microbiological processes, prevention of soil fertility.
In the course of the experimental work the effect of herbicides on soil biological properties were examined in different maize (Zea mays) cultures. We wanted wished to know that how the herbicides affect the quantity change of soil microorganisms, the life of different physiological groups of bacteria and the activity of microorganisms. A small pot experiment was set up in 2008 with the application of two herbicides - Acenit A 880 EC and Merlin 480 SC – in the breeding house of the Department. The moisture content and nutrient supply were at optimal level in the experiment.
On the basis of results the following can be stated:
1. It can be stated that the two herbicides and all their doses affected negatively the number of total soil bacteria, the
inhibiting effects were significant. The quantity of microscopical fungi increased by the effect of Merlin 480 SC and decreased in the treatments of Acenit A 880 EC.
2. The Acenit A 880 EC had stimulating effect on the nitrate mobilization. The CO2-production was stimulated by the basic doses of herbicides; the other treatments did not influence the CO2-production significantly.
3. The quantity of microbial biomass-carbon –except for only one treatment- decreased significantly by the effect of herbicides. Besides it, the quantity of microbial biomass-nitrogen increased significantly in the treatments of Acenit A 880 EC.
4. The biomass of test plant decreased in the treatments of herbicides, their quantities were smaller than in the control. In the pots treated by Merlin 480 SC, parallel with the increase of doses decreased the quantity of plant-biomass. -
The effect of breed and stage of lactation on the microbiological status of raw milk
37-45Views:340The microbiological quality of the milk is important not only for food safety, but it can also influence the quality of dairy products. The microbiological status of raw cow milk can be influenced by many factors. Our aim was to determine whether there was a difference between the microbiological quality of milk of two different cow breeds (Holstein Friesian and Jersey) kept and milked in the same conditions, and how the microbiological quality of the raw cow milk changed during lactation (beginning, mid, and end). Samples were taken and analysed in July, August and September in 2018 from two dairy farms in Hajdú-Bihar county. During the conducted studies, the total plate count (TPC), the coliform count, the Staphylococcus aureus count and the coagulase-negative Staphylococcus (CNS) count of raw milk samples were determined.
There was no significant difference (P>0.05) between the milk of the Holstein Friesian and Jersey breeds in the case of TPC. However, the mean coliform count of milk samples taken from Holstein Friesian cows was significantly lower (P<0.05) than the mean coliform count of milk samples taken from Jersey cows. S. aureus was detected in one of the twelve milk samples taken from Holstein Friesian cows, and in two of the eleven milk samples taken from Jersey cows. CNS was found in larger amount in milk samples taken from Holstein Friesian cows, and the difference was significant (P<0.05). Both TPC and CNS count were significantly higher (P<0.05) in individual milk samples taken at the end stage of lactation, than in samples taken in the earlier stages of lactation from Farm “A”. However, in the case of Farm “B”, there was no significant difference (P>0.05) in colony counts at different stages of lactation. S. aureus was only present in milk samples that collected from cows, which were at the beginning and middle stages of lactation. Testimg the hemolysin production ability of S. aureus strains isolated from the raw milk samples, only weak hemolysis was observed on blood agar. In case of antibiotic resistance testing, it was found that all strains were susceptible to cefoxitin, chloramphenicol, clindamycin, erythromycin, gentamicin, penicillin G, tetracycline and trimethoprim/sulphamethoxazole.
Based on the results of our studies, staphylococci were detected in a higher amount in the milk of Holstein Friesian cows, and coliform bacteria were detected in a higher number in the milk of Jersey cows. Summing up the results of the milk samples taken from the different stages of lactation in one of the farms, it can be concluded that higher TPC and CNS count could be detected at the end stage of lactation than in the samples taken from the earlier stages of lactation. The fact that at the end of lactation the microorganisms could be detected in a higher colony count may be related to the fact that teats could be damaged during lactation by the milking machine, which increased the chance of imvading the microorganisms into the udder.
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The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:165he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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The Role of Trichoderma in the Rhizosphere of Tomato Plants
67-69Views:73It is well established that microorganisms are closely associated with the roots of plants can directly influence plant growth and development. Species of Trichoderma, on the other hand are primarily studied for their ability to control plant disease. The ability of species of Trichoderma to directly promote or inhibit plant growth has been noted for many years.
Tomatoes were treated with different Trichoderma strains by seed treatment and soil inoculation. The Trichoderma were grown on malt-agar medium and conidia were washed off by sterile water for making suspension which contained 107 CFU/ml (colony forming unit/ml). The suspension was used for seed treatment and for the soil inoculation by watering as well. The artificial soil inoculation was made by Trichodermas growing on grounded maize were mixed in mould and tomato seed were sown in it. Tomato seeds were also sown in bags made of close-meshed material which allowed the soil microorganisms to colonize the roots but it simultaneously protected the roots from soil contamination. Roots were put on Trichoderma selective medium to check the root colonization of the Trichoderma.
The tomato plants were bedded out in a field in four repetition. After harvesting by hand the results supported by statistics shown that there was significant differences between the yield of the untreated and treated tomato plants by Trichoderma strains. -
The effect of keeping technology on the microbiological status of raw milk
67-75Views:136The importance of the quality of raw milk increased after Hungary had joined to the EU. On delivery of raw milk, the microbiological quality, especially total plate count of the milk is very important. Twenty-two farms (7 large, 4 medium-sized, and 11 small farms) were included in the study. We considered the different farm size, keeping- and milking circumstances during the selection of farms. The examined large farms use loose housing system (cubicle, deep litter) and milking parlour. Most of them use preand post-milking disinfection. In the medium-sized farms, loose,
deep litter and tie-stall housing system, as well as milking parlour, pipeline milking and bucket milking occurred. All of them use preand post-milking disinfection. Small farms use tie-stall housing system, bucket milking and udder preparation by water. Unfortunately, they do not use pre- or post-milking disinfection. In the large and medium-sized farms mainly Holstein Friesian, in the small farms Hungarian Simmental breeds can be found.
The aim of our research was to examine the microbiological status of the raw milk produced in dairy farms (total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, psychrotroph bacteria count, furthermore yeast and mold count); sources of the contamination; connection between the microbiological quality of produced milk and housing-, milking technologies of farms; furthermore the hygienic circumstances of milking and milk handling of the farms, by the examination of coliform bacteria and Escherichia coli contamination.
During the examination of the connection between the different farm sizes, various housing- and milking forms and the microbiological characteristics we observed similar tendencies in the case of total plate count, coliform count, yeast and molds count, furthermore psychrotroph bacteria count. The value of these parameters was significantly higher in small farms, and infarms which use tie-stall housing forms, bucket milking, udder preparation with water, and which do not use pre- and post-milking disinfection.
The results showed that besides cooling, the milking procedure and the type of udder preparation had the largest effect on the total plate count. Statistical analysis shows that in medium and small farms the combination of pipeline milking – tie stall housing system – disinfectant preparation of the udder; in large farms the combination of milking parlour – loose cubicle housing system – dry preparation of the udder are the most appropriate in the aspect of the total plate count. We experienced that in farms where the hygienic instructions are not followed – and therefore
equipment used during the milking and handling of milk is very contaminated – or rather the separation of mastitic cows’ milk is not appropriate, different microorganisms may contaminate the produced milk. -
Saccharomyces cerevisiae growth kinetics study dairy byproduct
169-172Views:243By guess, annual volume of milk whey is 185–190 million tons and this volume probably will increase next years. Whey has significant biochemical oxygen demand due to its high organic matter content so whey as sewage is one of the most pollutant by-products in the food industry. Apart from environmental pollution, benefit of several whey constituents for human health is another reason to utilize whey. Corn and potato, as well as the processing of milk in the food industry in large quantities of by-products generated by low cost, substantial quantities of starch and lactic acid, which are due to high biological oxygen demand are considered as hazardous waste. Some of them are destroyed sewage storage tanks, and those products are excellent substrates for the growth of microorganisms could be. The traditional nutrient solution optimization methods are solution and time-consuming and are not able to determine the real optimum because of the interaction of factors involved.
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Usage of microbiological products in the protection of the sunflower
36-40Views:85White mould is the most important desease of sunflower. We are not able to grow sunflower on the same area for 5-6 years, because of sclerotia. One of the protection methods is if we destroy the sclerotia in the soil with hyperparasite microorganisms, so we can reduce the comeback time of sunflower.
We carried out our farm size researches through 3 years, with the most important sunflower hybrids of Hungary in the area of Vásárhelyi Róna Kft. We can conclude that the microbiological products (Koni, Trifender, Mico’sol) effect positively on the yield of sunflower hybrids, but the weather (moisture) can significantly modify this effect.
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Identification of the Slovak traditional cheese “Parenica” microflora
227-239Views:266Numerous studies have demonstrated the higher accuracy, faster time-to-results and lower costs provided by MALDI Biotyper systems compared to classical methods. In this study, the culturable population of total count of bacteria, enterococci, coliforms bacteria, lactic acid bacteria (LAB) and microscopic fungi and yeasts from cow’s dairy products was identified using the MALDI-TOF MS Biotyper. Altogether, 50 samples of the Slovak cheese “Parenica” were examined. Total numbers of bacteria were cultured on Plate count agar at 37 °C for 24–48 h, aerobically; enterococci were cultured on Enterococcus selective agar at 37 °C for 24–48 h, aerobically; coliforms bacteria were cultured on Violet Red Bile lactose agar at 37 °C for 24–48 h, aerobically. The LAB were cultured on MRS (Main Rogosa agar), MSE and APT agar at 30 °C in microaerophilic conditions. The microscopic fungi and yeasts were cultured on Malt extract agar at 25 °C for 5 days, aerobically. Isolated strains (total 669) were subjected to identification by the MALDI-TOF MS. Among total count the identified bacteria mostly were Acinetobacter baumannii, Bacillus cereus, Micrococcus luteus and Staphylococcus warneri. Escherichia coli and Enterobacter cloacae were the most abundant coliform bacteria representatives identified. Coliform bacteria included Citrobacter, Hafnia and Klebsiella. Altogether three genera belonged to the LAB – Lactobacillus, Lactococcus and Leuconostoc were identified with Lactococcus lactis, Lactobacillus plantarum, Lactobacillus coryniformis, L. fructivorans and Leuconostoc mesenteroides were considered as the dominated LAB species in dairy products. Among yeasts, Kluyveromyces lactis, Candida zeylanoides and Yarrowia lipolytica were among the most isolated.
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Bacteria in the milk of sheep with or without mastitis- mini Review
47-52Views:373From a nutritional point of view, sheep milk is more valuable than cow and goat milk and the interest for sheep milk is increasing in many countries. However, sheep milk is easily contaminated during milking, handling, and transport and it is an ideal medium for bacterial propagation. Consequently, sheep milk spoils quite quickly. The proper, clean handling of milk is not only of sanitarian interest, but it also serves the farmers’ interests, because contaminated milk may not be distributed, and is unsuitable for producing good quality products. Following this technological trend, this review addresses the bacterial composition of sheep milk with and without mastitis. Even though sheep milk contains a lot of bacteria, this review article highlighted total plate count, Enterobacteriaceae, coliform, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Campylobacter, Salmonella spp. and Streptococcus spp. Mastitis in sheep is a vital cause of mortality, reduction in milk production and early culling. The reported risk factors for mastitis in sheep were age, a case of mastitis, breed, husbandry systems, and location. The main priority should be implementation of programs to minimize human pathogenic bacteria and mastitis in raw ewe milk.
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Effects of some herbicides on the microbiological characteristics of soil nitrogen cycle under maize plantation
93-100Views:89Nitrogen is a key element for the living organisms and influence not only for the quantity but for the quality of the yield, considerable. Availability of nitrogen from the soil is influenced by several microbiological processes of the Nitrogen-cycle. Among the intensive agricultural production the herbicide application cannot be omitted more information needs therefore about the inhibitor effect of herbicides on the different microorganisms.
An experiment was set up on calcareous chernozem soil under maize culture. Effect of four different herbicides (Acenit, Frontier, Merlin, and Wing) was investigated. The effect of herbicides was measured to four microbiological parameters of the Nitrogencycle (abundance of nitrifying bacteria, nitrate solubilisation, biomass nitrogen and urease enzyme activity). There were singledouble- and five times of recommended doses of herbicides applied for two onsecutive vegetation periods.
From the results of the different doses of herbicides, the following can be stated:
– The Acenit has a stimulating effect on nitrifying bacteria in general. The Frontier and Merlin also influenced the quantity of nitrifyers, however in certain cases decreased in another cases increased the number of bacteria.
– The double doses and five times doses of herbicides was found to be increasing the nitrate content of soil, -especially in 2006.
– The quantity of microbial biomass nitrogen increased in the 60% of treatments and decreased in the 40% of the treatments.
– Except of the result of Wing in 2006 and Merlin in 2005, the effect of simple dose herbicides was the smallest on the urease enzyme activity. According to the results the effect of Merlin was positive; the effect of Wing was negative on the soil enzyme’s activity.
Regarding the application of four different herbicides in three different doses on the microbiological parameters of soil (at two consecutive years-) in 39% of the treatments has resulted a significant inhibitory effect, 28% of the treatments, however have significant stimulating effect on the parameters measured. More than 50% of the inhibitory effect was measured in case of the Wing, at more than 50% of the Frontier the microbiological parameters have not changed. -
Definition antioxidant activity of selenium-enriched food sprout, as well as their microbiological analysis
25-30Views:255In this present study, we prepared selenium-enriched food sprouts, where the antioxidant capacity was analysed, we also determined their microbiological status. We took into account the fact, we choose micronutrients to our treatment, that selenium can be delivered to the body by a small amount with the most widely consumed food.
We focused during our research to determine that the increasing concentrations of selenium treatment, in which we used sprouts, knowing fully well that it has an impact on aboriginal antioxidant capacity of sprouts, which is mainly due to high vitamin content of sprouts.
Furthermore, we think it is important to make microbiological analysis, because germination conditions, for example temperature, pH, all this will create an ideal environment for the growth of microorganisms. So we had goal to determine, how the used selenium concentration affect the total plate count, coliform bacteria count and Staphylococcus aureus count of sprouts.
We determined the aboriginal water-soluble and lipid-soluble antioxidant capacity of sprout with the PHOTOCHEM chemiluminometer and we applied pour plate technique for the mapping of the mycrobiological state of sprouts.
Experimental results are evaluated, we state that increasing concentrations of selenite or selenate treatment, is primarily water-soluble antioxidant capacity of sprouts was affected. The water-soluble antioxidant capacity of wheat sprout was much higher than the measured values in pea sprout, this may be linked to what we measured. That is much higher ascorbic acid content in case of wheat sprout, which is well known as one of the most important antioxidant properties compounds of wheat sprout.
We conclude from the results of the microbiological, that the highest concentrations of selenite or selenate treatment has a relative significant anti-microbial effect in case of wheat sprouts. Coliform and total plate count showed no clear decreasing tendency, although the values of treatments in both cases obtained were below the control values.
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Energy production systems of phototroph microorganisms (classification of photobioreactors)
35-39Views:72In the field of alternative energy sources there is an argue in the comparison of its effects on the benefits and disadvantages to the economics and the environment. New studies are born which are in contradiction with each other. The demand for bioenergy feedstock is growing rapidly however there are the environmental problems caused by the extending energy crop plantations. There is such a significant need for land to grow traditional energy crops on (rape, soy, palm-oil, sugarcane, etc.) that the food purpose agricultural capacity could be in danger. Probably the extensively
growing energy crops play a role in the very high prices of food. In some countries like China for example laws prohibit the use of food based crops such as corn for energie production. In the case of corn based ethanol production the cost only for the feedstock itself is over 60% of the whole preparation costs which significantly effects the entire economy of the energy productions process.
The microalgaes however have a huge biotechnological potential and their production is notably cheaper then the traditionaly grown food crops growing expenses. They play a significant role as feedstocks in todays industrial production in such fields as comestible production, cosmetics, pharmaceutics and biotechnology especially in biofule production. In the field of economy the major aspects here are also the technological designs
and the construction. For the future industrial production the closed type systems seem to have more advantages compared to the open, pond-type systems. For high value material production the more innovative and more easily developed closed photobioreactors are the profitable regardless the vast techological designs used in the construction. -
Microbiological quality of bulk tank raw milk from two dairy farms in Hajdú-Bihar County, Hungary
105-112Views:327Two main channels have been identified to be responsible for microbiological contamination of raw milk and milk products. Firstly, contamination has occurred due to udder infection from the cow or the blood which harbours most bacteria that come in contact with the raw milk. Secondly, via external factors (may include faeces, skin, contaminated water, environment etc.) which are associated with the operation of milking. There is direct contact with the milk and/or surfaces before, during or after the milking, posing public health risk and economic decline. The aim of this study was to examine the bacteriological quality of bulk tank raw milk samples collected from two different size dairy farms (Farm 1 and Farm 2) of different housing forms (cubicle loose and deep litter) in Hajdú-Bihar County, Hungary in July, 2017. Three samples were taken from each farm, and the total plate count, coliform count, Escherichia coli count, Staphylococcus aureus count, and yeast and mould count were determined in them.
The results clearly showed low level of all measured bacteria group load in Farm 1 samples in comparison to Farm 2 with the exception of coagulase-negative Staphylococcus (CNS) which represented high level in general, indicating significant difference (P<0.05). The mean value of total plate count in Farm 2 samples was higher (1.0 × 105 CFU/mL) than Farm 1 samples (2.8 × 104 CFU/mL). There was a significant difference (P<0.05) in mean count of coliforms in raw milk samples between Farm 1 and Farm 2. Similarly, results of E. coli were significantly different (P<0.05) with mean count of 1.44 × 102 CFU/mL and 2.02 × 103 CFU/mL for Farm 1 and Farm 2 respectively.
Results of Staphylococcus aureus also showed significant difference (P<0.05) with mean count of 9.7 × 101 CFU/mL for Farm 1 and 6.28 × 102 CFU/mL for Farm 2. The mean of mould count recorded was 1.07 × 102 CFU/mL and 4.93 × 102 CFU/mL for Farm 1 and Farm 2 respectively. The recorded mean of yeast count was 1.68 × 103 CFU/mL and 3.41 × 103 CFU/mL for Farm 1 and Farm 2 respectively; however, both farms showed no significant difference (P>0.05) in terms of mean of mould and yeast count. Although Farm 2 produced six times lower milk quantity than Farm 1, the measured microbial parameters were high. Both farms’ microbiological numbers were higher above the permitted limit values as stated by Regulation (EC) No 853/2004, Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.).
This could be an indication of non-conformance to effective GMP, ineffective pre–milking disinfection or udder preparation, poor handling and storage practice, time and temperature abuse and inadequate Food Safety Management System Implementation. Therefore, our recommendation is as follows; establish control measures for pre- and postharvest activities involved in the milking process which would be an effective approach to reduce contamination of the raw milk by pathogenic microorganisms from these farms, strict sanitation regime and hygiene protocol be employed and applied to cows, all equipment, contact surfaces and minimize handling of the milk prior, during and after milking. This will also serve as scientific information to the producers for continual improvement in their operations.
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Búzaminták fungicid szermaradvány ellenőrzése és gombafertőzöttségének kimutatása
45-51Views:88To protect crops with chemicals against different microorganisms are very important because residues of toxins could pollute infected grains. This could have the meaning of serious danger to the human and animal health. Similarly important is knowledge of details about presence or absence of any practically applied protective compounds. Experimental studies were performed on fungicide treated cereals of the Cereal Research Non-Profit Company, Szeged. Chromatographic methods were used (GC and some GC-MS) to find low level fungicide residues, microwave-assisted isolation of ergosterol was followed by HPLC to detect possible fungal infections and a more detailed microbiological analysis completed the work. Concerning the dry year 2000, no fungicides were found in the samples (by November), the ergosterol level was found to be less than 8 ppm, meaning good quality, coupled with an extremely low level of fungal infections. This was confirmed by direct microbiological testing. In comparison with the microbiological results from 1999, a negative difference can be recognized, most probably due to the rainy weather throughout the year.
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Alternatives of sewage sludge use in the crop production
83-87Views:118The produced plants reduce the greenhouse effect because they fix CO2 that contributes to the causing of the greenhouse effect with about 50%. The production of fertilizers is not only a costly process but it needs a considerable energy at the same time. Nowadays, the reduction of the proportion of the fertilizer is significant. One of the reasons of this is that during the production such by-products are produced in a big quantity in which the necessary vegetal nutrients can be found in a considerable measure these enrich the organic matter of soil. The latter is essential condition for the microorganisms in the soil, without which the sustainable plant cultivation can not be achieved. Besides high prices of artificial fertilizers the utilization of the wastes is economically justified. Finally the other reason for the reduction of a usage of artificial fertilizer is that the wrong use of the fertilizer may cause environmental pollutions. I examined the cultivation application of the sewage sludge in laboratory circumstances during my work.
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The effect of bentonite on the quantity change of soil microorganisms, the CO2-production and the activity of saccharase enzyme
287-293Views:96We examined the impact of bentonite – the perspective improving material of sandy soils – and treatments of livestock manure composted with bentonite on sandy soils, within the framework of a small-plot experiment.
The adjustment of the experiment was made on the Experiment Site of the Nyíregyháza Research Centre of the University of Debrecen, Centre of Agricultural Sciences (UD CAS). We collected soil samples from parcels treated with increasing bentonite doses (5, 10, 15, 20 t/ha) on the one hand, and from the parcels treated with livestock manure composted with increasing doses of bentonite, on the other.
We performed laboratory research in the soil microbiology laboratory of the Soil Science Faculty of UD CAS DAS, during which we determined the total number of bacteria, the quantity of microscopic fungi, the number of cellulose-decomposing bacteria, the CO2-production of the soil and the activity of saccharase enzyme.
During the evaluation of the examinations, we made a statistical analysis using SPSS 9.0. We determined the average of measurements, the standard deviation of controls, the standard deviation, the significance value and we also performed a correlation analysis.
Concerning the impacts of bentonite treatment and the treatment of livestock manure composted with bentonite on the examined microbiological features of sandy soil, we can summarize the following:
• Our results prove that the microbiologic activity of the soil has increased owing to the impact of bentonite treatments regarding total number of bacteria and the quantity of microscopic fungi. ”Pure bentonite” treatments – although not significantly in every case – increased these values, but larger doses decreased them. The treatments of livestock manure composted with bentonite resulted in a larger increase regarding both parameters.
• The number of cellulose-decomposing bacteria was increased by the low doses of both series, and was decreased by the higher doses. Higher bentonite doses decreased it in a higher – significant – degree than those of treatments of livestock manure composted with bentonite, whose low dose caused salient number of bacteria.
• Regarding the carbon-dioxide formation, we have experienced an increase even in the case of low dose treatments (nevertheless, the increase did not prove to be significant), but – similarly to the quantitative changes in the number of cellulose-decomposing bacteria – the large doses of both series of treatment decreased the CO2-production of the soil.
• Bentonite also increased the activity of saccharase enzyme significantly. We learned that”pure bentonite” treatments increased the activity of the enzyme to a higher degree than composted treatments. Moreover, it can be stated that the treatments of larger doses of both bentonite and livestock manure composted with bentonite have decreased the enzyme activity – not significantly, though.
• Based on the correlation analysis, it can be stated that as an impact of the treatments, the microbiological activity of the soil has also increased with the increase of the number of soil microbes, as in both treatment series we have experienced a tight positive correlation (r=0.81-0.82) between the change of total number of bacteria and the CO2-production of soil. In both treatments, there was a medium correlation between the total number of fungi and soil respiration (r=0.63-0.63). Furthermore, it can be stated that the usage of organic manure had a positive effect on the activity of cellulose-decomposing bacteria, as in this treatment series there was a positive correlation (r=0.65) between this physiological group and CO2-production. Both treatments prosperously impacted the activity of saccharase enzyme, because there was a medium correlation (r=0.62-0.64) between the activity of the enzyme and soil respiration. -
Effects of cultivation methods on some soil biological parameters of a meadow chernozem soil (Vertisols)
61-66Views:85The effect of extended drought conditions on soil, the unfavourable cultivation technologies and the application of chemicals have been enhancing the processes of physical and biological soil degradation, so the fertility of soil is gradually declining.
The effects of two cultivation methods – traditional ploughing (TP) and conservation tillage (CT) – on the biological activity of a meadow
chernozem soil were examined in a long term experiment. Different parameters of the biological activity of soil were determined. These are
the numbers of total bacteria, microscopic fungi, aerobic cellulose decomposing bacteria, as well as the activities of some important soil
enzymes and CO2 production.
Conservation tillage seemed to be a more favourable cultivation method for the majority of microorganisms, the activities of urease and
dehydrogenase enzymes and CO2 production, compared to the traditional ploughing system. These parameters increased significantly,
especially in the upper layer of conservation tillage plots. Concerning the plant cultures, the majority of microbiological parameters were
higher in the soil of vetch (Vicia sativa L.) depending on the cultivation methods, so involving the pulses to the crop-rotation seems to be
very important in this soil type.
According to the ninth year’s results, the importance of conservation tillage as a means of protecting the soil biological activity in meadow
chernozem (Vertisols) can be established; it was proven by microbiological investigations. -
Effect of agrotechnical factors on the activity of urease enzyme in a long term fertlization experiment
43-48Views:150The soil is a natural resource, the fertility preservation is an important part of the sustainable development. We have to monitor the transformation dinamics of the organic nitrogen-containing substances, to get accurate information about the changes of the nitrogen cycle in the soil.
Physical and chemical properties of the soil and the microorganism effect on the organic matter in the soil – in addition to the composition of organic matter. Wide variety of extracellular enzymes are present in this decomposition. These enzymes help in the transformation of the macromolecules to transforming low molecular weight compounds so they will be available during the assimilation.
The urease enzyme, catalyzes the hydrolysis of urea to CO2 and NH3. The urease is widely spread in the nature, it is present in the microorganisms, plants and animals.
We found that the soil moisture content, the rotation and the fertilization affect to the amount of urease in spring. Furthermore, we get significant difference between the irrigated and non irrigated samples in the second period of the year. Based on our results we can state that the activity of urease was higher in spring 2014.
The objective of our study was to present how the different agronomic factors affect on the activity of urease in a long term fertilizationexperiment.
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Soil Biological Activity within Integrated and Ecological Management of Soil
47-52Views:88The effects of the integrated (IS) and ecological (ES) management of soil on chosen parameters of soil biological activity were investigated in the period 1999-2000. The following characteristics were determined: biomass of microorganisms (Cmic), dehydrogenase activity (DHA), an amount of potentially mineralizable nitrogen (Nbiol), and nitrification intensity. Soil samples were collected from a stationary field experiment established in 1990 on gley brown soil at the Experimental Station of Slovak Agricultural University, Nitra. For each field with a different crop rotations two fertilization treatments were selected: (a) no fertilization and (b) use of manure for silage maize and, within IS, also mineral fertilizers. There was a statistically significant difference at α = 0.05 in the amount of biologically released nitrogen (Nbiol) between both systems and in the nitrification intensity in favour of ES. A higher amount of microbial biomass (Cmic) was noted for ES but without statistical significance. Cultivated crops and the timing of soil sampling were found to have the greatest effect on all the parameters observed in individual experimental years and within the two systems of soil management.
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The effect of different microbial preparations on some soil characteristics
83-86Views:113In pot experiment the effect of different microbial inoculants and their combinations with NPK fertilizer and wheat straw on some soil properties (physical, chemical, and microbiological parameters) were studied. The experiment was set up in 2011 at the Institute of Agricultural Chemistry and Soil Science, in a three replications, in a random block design. The studied soil type was calcareous chernozem soil from Debrecen (Látókép) with ryegrass (Lolium perenne, L.) test plant.
At the end of the experiment in our laboratory the nitrate-nitrogen content of soil, the AL-soluble phosphorus and potassium content of soil, the urease enzyme activity of soil, the total number of bacteria and the number of microscopical fungi were determined.
The results of the study were the following:
– The straw treatment and the straw + biofertilizer combinations influenced positively the nitrate content of soil.
– The NPK fertilization and the straw+bacterial fertilizer combinations had significant positive effect on the AL-soluble phosphorus content of the soil.
– The biofertilization and the straw+biofertilizer combinations stimulated the AL-soluble potassium content of soil occasionally.
– The total number of bacteria was influenced by the NPK fertilization, the bacterial fertilization and the straw+bacterial fertilizer combinations significantly.
– In case of the number of microscopic fungi caused in some cases significant changes the NPK+bacterial fertilizer and straw+bacterial fertilizer combinations.
– The soil urease enzyme activity was increased in all cases strongly especially by the straw+bacterial preparation combinations. -
Antimicrobial effect of dried sage on the microbiological state of fresh Hungarian sausage
189-192Views:170The purpose of this study was to evaluate the microbial effect of dried sage (Salvia officinalis L.) on the traditional Hungarian sausage. We added 0.5, 1, 1.5, and 2 w/w% of sage to the sausages and tested them on the 0th, 7th and 14th day. The added dried sage had no effect on the tested microorganisms, but the sage extract inhibited Salmonella, Enterococcus faecium and Staphylococcus aureus.
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Overview of the evolutionary history and the role in citric acid production of alternative oxidase
83-88Views:148All organisms are exposed to countless environmental effects, which influence in a disadvantageous way their life processes. They continuously adapt to the changing conditions and respond to the stress impacts by defence mechanisms. Through different signal transduction pathways they are able to increase or decrease the expression of their genes and consequently modify their metabolic processes. My interest focuses on alternative oxidase (AOX) enzyme whose expression is often increased under biotic and abiotic stress. The so far proven and putative functions of the AOX play a role in the ability of organisms to adapt to different conditions, such as heavy metals accumulation, pathogenic infection, oxidative stress and lack of oxygen or nutrients.
AOX is a member of the di-iron carboxylate protein family. Members of the di-iron carboxylate protein family are present in all kingdoms of life. They are considered to have ancient origin. It is believed that their sulfide-resistant and oxygen-reducing ability played a role in the survival of organisms during the transition between the anaerobic and the aerobic world. It is assumed that the AOX arose in eukaryotes through a primary endosymbiotic event, and this event made possible the development of mitochondria. Afterwords, vertical inheritance, and secondary and tertiary endosimbiotic events led to its spread among eukaryotes. It is assumed that bacteria obtained AOX by horizontal gene transfer from plants.
AOX-catalyzed alternative respiration plays an important role in the operation of energy-producing and biosynthesizing system of microorganisms. In these cases, the regeneration of reduced cofactors is an essential condition, and therefore may be rate-limiting for biotechnological processes, including the citric acid production.