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Lack of polymorphism of the agouti signaling protein (ASIP) gene among four different brown hare (Lepus europaeus Pallas 1778) populations
81-85Views:140The brown hare (Lepus europaeus Pallas 1778) is a common palearctic and a popular game species therefore it has been an obvious subject for population genetic studies since the second part of the 20th century. Among the several mitochondrial DNA studies some have been carried out concerning nuclear genes as well. The agouti signaling protein gene (ASIP) is involved in regulating the synthesis of eumelanin and pheomelanin in melanocytes of mammals. Though many studies focused on it in relation with several mammalian species, minimal information is available on this topic concerning the brown hare.
Here we present a short communication concerning the agouti signaling protein (ASIP) gene in four different country’s L. europaeus populations, namely Lithuania, Hungary, Serbia and Georgia. N=45 tissue samples have been investigated from overall 17 sampling sites of the different countries. There has not been found any polymorphism among the sequences. In an alignment with other Leporid species’ partial ASIP sequences downloaded from ENA we have found that based on a 178 base pairs long DNA sequence the haplotype of our samples contains three other Lepus species as well. This is concordant with the findings of a previous study focusing predominantly on the European rabbit (Orycto lagus cuniculus Linnaeus 1758) and the several mutations of its ASIP gene.
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Effect of agrotechnical factors on the yield of the Grolim asparagus (Asparagus officinalis L.) hybrid on acidic sandy soil
43-48Views:223Research of blanched asparagus has begun at the University of Debrecen Institutes for Agricultural Research and Educational Farm Research Institutes of Nyíregyháza in 2011. Establishment of the plantation took place in May 2011. The Grolim hybrid was used in the trial, 16 medium plot trial area has been formed under field conditions, with four repetitions and 36 m2 plot size. In the course of our studies, the effect of different nutriment supply methods (untreated, manure, sheep manure compost, fertilizer) has been analysed on the spear yield of the Grolim asparagus hybrid between 2013 and 2017.
In our studies, the beginning of vegetative growth has been recorded upon the constant presence of 10 °C of average soil temperature in the case of the Grolim asparagus hybrid. The beginning and length of spear harvesting are both influenced by the time and dynamics of initial development in spring. During the analysed period, the dates of spear harvesting were various, the earliest being on 23rd March, 2014 and the latest on 23rd April, 2015; the rest of the three years have been varied within this one month interval. The total of heat units required for the vegetative development of spears has been determined; it provides important information for cultivation practice.
Spear yields turned out to be hectic during the analysed period. In 2013 and 2014, yields have surpassed the amount of 50 kg/harvest period/plot in the case of every treatment version. However, in 2015 a significantly lower specific yield has been recorded due to the unfavourable weather conditions in spring; a yield decline of nearly 50% was recorded in the case of the control treatment compared to the previous years. Yield was also lower in the rest of the fertilization treatments compared to 2014; however, in these cases, the degree of yield decrease was around 5–10%, which suggests the yield stabilising effect of fertilization. In 2016, a slight yield increase was measured in comparison with the base year. In 2017, there was a decline of yield in the control treatment; however, the different fertilization treatments resulted in yield increase as compared to previous years.
On the basis of our studies, it is clear that the best yield results have been provided by the artificial fertilization treatment in all of the five analysed years. It was followed by the sheep manure compost and manure treatments in terms of their effect on spear yield. During the three harvesting periods, the lowest yield on acidic sandy soil was recorded in the case of the control treatment. The most remarkable effect of nutriment treatments has been realised in terms of the decreased deviation of yield results, which perfectly represents the yield stabilising effect of nutriment supply in the case of perennial crops – asparagus – as well, even on a poor nutriment supply characteristic sandy soil.
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Fish consumption of the great cormorant in the area of Hortobágy Fish Farm
81-84Views:133The cormorant (Phalacrocorax carbo) is a bird species that nests sporadically but in colonies, besides larger fishponds and rivers in Hungary. The number of its individuals has been increasing during the last two decades. The species eats solely fish, therefore it can cause serious depredation of the fish stocks in fishponds of intensive system and after the freezing of the ponds, in larger rivers, which are not yet frozen. The aim of our research is to reveal the damages the birds can cause on the studied areas and the extent of the losses the Hortobágy Fish Farm Co. has to realize.
Our studies were carried out between April 2012 and November 2013. During cleaning, the investigation of the crop contents of the birds and their biometric studies were conducted. The results revealed the diverse nutrition base of the species. In the samples, we have determined 300 identified fish individuals; in 289 cases, the exact fish species were determined, too. Although statistical differences were found between the given pond unit and the consuming of the corresponding fish species (P=0.05% beside), this cannot be considered as absolutely certain.
Our investigation is of considerable significance in terms of nature preservation and not least, it has substantial financial concerns too. Under the ever harder fish production conditions, the presence and thus the permanent nutrition of the bird affect the fishermen. In favour of the maintaining of the natural ecological balance and the income of the fishers, the elaboration of adequate protective and efficient preventive processes will be necessary in the near future.
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GC-MS studies to map mechanistic aspects of photolytic decomposition of pesticides
11-16Views:82Transformation of pesticides in the environment is a highly complex process affected by different factors. Both biological and physical-chemical factors may play a role in the degradation, whose ratio depends on the actual environmental conditions.
Our study aims to reveal specific details of photolytic degradation of pesticides as important soil contaminants. Significance of these studies is enhanced by the fact that pesticide decomposition may contribute to soil degradation, and have harmful biological effects by degrading to toxic products. The toxicity of the examined pesticides is well known, however very little information is available regarding their natural degradation processes, the quality, structure and biological impact of the degradation products.
The photolytic degradation of frequently applied pesticides of distinctive types (acetochlor – acetanilide, simazine – triazine, chlorpyrifos – organophosphate, carbendazim – benzimidazole) was investigated. A special, immerseable UV-light source was applied in order to carry out photodegradation. The degradation processes were followed by thin layer chromatography (TLC) and mass spectrometry coupled with gas chromatography (GC/MS). EI mass spectrometry was used to identify the degradation species.
Each of the studied pesticides underwent photolytic decomposition, and the detailed mechanism of photolytic transformation was established. At least four degradation species were detected and identified in each case. Loss of alkyl, alkyloxy, amino-alkyl and chloro groups might be regarded as typical decomposition patterns. Deamination occurred at the last stage of decomposition. -
Studies on the development of food attractants catching noctuid mouths (Lepidoptera: Noctuidae)
86-91Views:162The monitoring of the most dangerous noctuid pests (Lepidoptera: Noctuidae) can be performed by species specific pheromone traps. Recently the development of traps catching female moths became the main objective of the studies. We studied the synergistic effect of vine and beer as natural additive on the effectiveness of baits containing isoamyl alcohol and acetic acid in Forró and Debrecen-Ondód. The addition of vine and beer had positive effect on catches and it was significant in case of two dangerous pest species Agrotis segetum and Lacanobia oleracea. The synergistic effect was also significant with regard to the number of detected species especially in case of lower abundances and rare species.
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The occurrence and phenology of moth pests in different granary of Borsod-Abaúj-Zemplén County
70-75Views:90The aims of my studies were the followings: primarily to find correlation between the conditions of granary and the occurrence of moth pests. Secondly I studied the effect of disinfection on individual numbers in the population of moths. My studies were started in May 2009 in six different places of Borsod-Abaúj-Zemplén County. Indianmeal moth (Plodia interpunctella) and Mediterranean Flour Moth (Ephestia kuehniella) traps with pheromone were installed in four repetitions as well as Angoumois Grain Moth (Sitotroga cerealella) traps in also four repetitions. Control traps without attractant were also placed at every place.
From my researches, it became clear that the disinfection alone is not enough to protect cereals from moths. As in the case of crop protection, we need to apply integrated pest management.
We have to make differences between preventive protections from moth pests and the elimination of them by chemicals.
Up to my opinion, the regular checking and cleaning of the granary are important as well as the prognosis of the possible occurrence of moths. The prognosis is considered important because the studied moths do not feed at the adult stage or only at a low level. However, the caterpillars coming from the eggs placed by females can cause a significant damage in the stored cereals.
The studied sex pheromone traps are proved to be useful for the reduction of number of moths since the traps caught lots of them. These traps are relatively cheap because only the temporarily changes of pheromones increase the cost. -
The comparison of sperm motility and density in four different goldfish (Carassius auratus) types
135-140Views:161Different goldfish types play an important role both in ornamental fish farming and science. Considering its historical background, the goldfish is a suitable model animal for the study of artificial selection as well as for developmental biological studies. Sperm motility and cell density is an important parameter in determining sperm quality. The aim of our study was to examine the effects of different goldfish types on the sperm quality. Several sperm motility parameters (progressive motility (pMOT, %), straight line velocity (VSL, μm s-1), curvilinear velocity (VCL, μm s-1), linearity (LIN, %), amplitude lateral Head Displacement (ALH, μm), Beat Cross Frequency (BCF, Hz)) of four different goldfish types (Common goldfish-“wild type” N=5, Shubunkin N=4, Black Moor N=4, Oranda N=5) was compared during 60 hours (at 12-hour intervals) at refrigerated storage (4 °C). The variability of sperm density was also investigated in all types. A similar cell concentration was determined in the four goldfish variants (Common goldfish 2.01*1010 ± 3.46*109; Shubunkin 1.71*1010 ± 3.25*109; Black Moor 1.66*1010 ± 3.02*109; Oranda 1.56*1010 ± 5.83*109). Statistically significant difference between the 4 goldfish types in the motility parameters and cell density was not noted. However, a decreasing tendency in Black Moor sperm motility parameters (pMOT, VCL and VSL) was observable, as well as a reduced spermatozoa density in Oranda was also recorded. Our results can contribute to the improvement of the common hatchery propagation of goldfish. Future studies can add more evidence of the possible effects of artificial selection on the reproduction in different goldfish types.
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75 years of the chestnut blight fungus Cryphonectria parasitica (Murr.) Barr in Europe
82-85Views:197The chestnut blight fungus Cryphonectria parasitica is a native pathogen in East Asia and has been introduced into North America and Europe. Historical records and population genetic studies revealed at least three major introduction events from Asia into Europe.
Nowadays, chestnut blight is present in almost the entire distribution range of European chestnut, i.e. from the Iberian Peninsula to the Caucasus. The C. parasitica population in most countries has been studied in respect to the diversity of vegetative compatibility (vc) types and the occurrence of hypovirulence. The vc type diversity of the different populations varied considerably. Typically, a high diversity of vc types has been found in areas with a long history of chestnut blight and where sexual recombination between divergent genotypes commonly has occurred. On the other hand, newly established populations often showed a low diversity with only one, or a few vc types present.
Hypovirulence, i.e. the occurrence of C. parasitica isolates infected by Cryphonectria hypovirus 1 has been found widespread in Europe. Natural dissemination and active biological control applications have lead to a high prevalence of the hypovirus and to the recovery of many chestnut stands. Virulent cankers became hypovirus-infected within a short time and ceased expansion. There is concern that the diversity of vegetative compatibility types could increase in Europe through sexual reproduction between C. parasitica genotypes originating from different introductions. A higher level of vegetative incompatibility would not only hamper hypovirus spread within a population but could also select for lower virulence in CHV-1 and subsequently lead to an erosion of biological control. Recent studies, however, indicate that the vc type barriers are not so restrictive than previously assumed and that so far no evidence for an erosion of biological control system in high diversity populations can be observed.
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Microbiological status of bulk tank milk and different flavored gomolya cheeses produced by a milk producing and processing plant
73-78Views:246The microbiological quality of milk is important not only for food safety, but it can also influence the quality of dairy products. In this study, our aim was to assess the microbiological status of the bulk milk of a milk-producing farm, and some natural and flavored (garlic, dill, onion) gomolya cheeses made from pasteurized milk produced by their own processing plant. We determined the number of coliform bacteria, Escherichia coli, Staphylococcus aureus, and molds of three milk and eight cheese samples. The tests were conducted between July and September, 2017.
In bulk milk, the mean coliform count was 3.83±0.17 log10 CFU/ml; the mean E. coli count was 1.38±0.14 log10 CFU/ml; the mean mold count was 3.74±1.30 log10 CFU/ml; and the S. aureus count was <1.00 log10 CFU/ml, respectively. The mean coliform count in gomolya cheeses was 3.69±1.00 log10 CFU/g; the mean E. coli count was 2.63±0.58 log10 CFU/g; the mean S. aureus count was 3.69±1.35 log10 CFU/g and the mean mold count was 1.74±0.37 log10 CFU/g. The amount of coliforms detected in different flavored gomolya cheeses were significantly different (P<0.05). More than 10 CFU/g of E. coli was found only in the dill flavored cheeses, and S. aureus was found only in dill (3.66±1.86 log10 CFU/g) and onion (3.71±0.52 log10 CFU/g) flavored gomolya cheeses. Based on the obtained results, it was found that the amount of coliform bacteria and E. coli in bulk milk exceeded the limit set in regulation of the Hungarian Ministry of Health (MoH) 4/1998 (XI. 11.) and the amount of S. aureus was below the limit. For gomolya cheeses, the S. aureus count exceeded the limit. The amount of coliform bacteria remained above the limit in cheeses, except for the garlic flavored gomolya cheese. In cheeses, a larger E. coli count was detected than in the bulk milk, but there is no specific limit for cheeses in the regulation. The mold count exceeded the limit specified in the regulation in cheeses, but a lower value was detected relative to milk.
The results show that, in the case of bulk milk and gomolya cheeses, certain detected quantities exceeded the limit values set forth in regulation of MoH 4/1998 (XI. 11.). The results indicate an inadequate microbiological state of the raw material and the finished products. The reasons for these are due to reduced technological hygiene or the inappropriate handling of raw material and finished products. In this study, we have summarized the results of our preliminary studies, which can provide a basis for further hygiene studies.
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Comparison of growth parameters of 40 varieties/clones short rotation coppice willow (Salix)
99-104Views:127The plantation of willow varieties was established in 2009. The 40 different varieties and clones of Salix were planted at a research field in Kolíňany (Nitra district). The paper evaluates results from the first growing season in 2009. The survival rate of planted cuttings ranged from 55.56 % to 100.00 % after the first year. The lowest survival rate was reached by Terra Nova variety. The stem numbers per plant ranged from 1.17 ±0.37 to 2.53 ±0.98. The average height of one-year old stem varied from 65.82 ±36.60 cm to 225.58 ±68.61 cm. The average stem diameter ranged from 6.90 ±2.63 mm to 14.34 ±3.39 mm. There were statistically very significant differences in parameters of stem height stem diameter and stem numbers per plant among studied varieties/clones. The statistic method used was analysis of variance ANOVA. The varieties were then divided into 6 groups according to their similarity in observed parameters after the first growing season by cluster analysis. The best results were reached by varieties/clones classified in the second group. The survival rate, stem diameter and stem height values of these varieties/clones were above average.
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The comparative evaluation of the preparedness and development of graduates
127-133Views:71The skill of dealing with people was given a special importance by the persons interviewed during my examination by means of questionnaires although most of the specialist works I
consulted did not pay too much attention to this fact. When asked about the particular fields of further studies, only a small proportion of the respondents regarded the development of their
personality important. I think to dissolve this paradox is necessary as without the development of personality it is impossible to improve interpersonal skills, that is one of the basic pillars of good performance at work. Regarding that it is the graduates who fill managerial posts, dealing with people belongs to one of their tasks.
Among the respondents if the first degree were from a college and not from a university, the greatest proportion of those studying further would live in Heves and Borsod-Abaúj-Zemplén counties. However, this connection cannot be proved in Budapest. Individual motivation is the main drive of studying further after obtaining a degree in a greater and greater proportion.
The graduates’ studying further is significantly directed to acquiring extra knowledge rather than a totally new profession and they get a deeper insight into the knowledge necessary for
their profession driven by their own motivation. -
Large Scale Measurement of the Total Antioxidant Capacity of Different hungaricum Fruit and Vegetable Samples with Photochem Chemiluminometric Device
300-304Views:81In our present study, we performed a quick test of the antioxidant capacity of domestic fruit and vegetable cultivars with a PHOTOCHEM® instrument (Analytik Jena AG, Germany). The instrument is special because it can measure both the total lipid-soluble antioxidant capacity (ACL method) and the total water-soluble antioxidant capacity. Another advantage of the system is its quick measurement. With similar instruments, such measurement can take several hours, while PHOTOCHEM® can perform it within a few minutes.
In our model studies, we examined the changes in the antioxidant capacity of sour cherry, green pepper, carrot, pumpkin, red beet and Brassica sp. cultivars. We aimed to determine how suitable the instrument is for quick, routine measurements in the case of the different horticultural products and which species have high antioxidant capacity values.
On the basis of our examinations, we can state that the PHOTOCHEM® system is suitable for routine, semi-automated measurements of the total antioxidant capacity at several vegetables and fruits. Besides this, the system can be used for testing the quality of food products, for monitoring storability in post-harvest technologies, and for special breeding programmes and for physiological and biotechnological studies. -
Phenometric studies on stalk juice and sugar contents of silo sorghum types
41-49Views:79Bioenergies (among them e.g. bioalcohol) can be solutions for the replacement of fossil fuels. For its production, plants with high sugar or starch content can be used. Juice pressed from the stalk of sugar sorghum has high sugar content (14-17%) that makes it suitable for bioethanol production. During our experiment, we examined 53 restorer male lines; among them 22 were silo type sugar sorghum. We studied the following traits: plant height, breeding time, level of foliation, stalk diameter, characteristics of stalk medulla, juice content of stalk, sugar content of stalk juice. According to examined characteristics, we selected six restorer male lines for studies in the forthcoming years: RL 1, RL 2, RL 3, RL 4, RL 5, RL 9, RL 12, RL 15, RL 18. Their stalk medullas were wet, stalk diameters were medium-thick, sugar contents of juices varied between 17 and 24% at the end of milk mature. Harvest was made in September, they can be classified into early maturation group. Male sterile female lines were the following: SL 1, SL 2, SL 3, SL 4, SL 5. The maintainer male lines were: CL 1, CL 2, CL 3, CL 4, CL 5. In Hungary, there are only a few male sterile female
lines, so we will use these lines for hybrid production during the next years. -
A study of product strategy priorities at the small enterprises of the North-Alföld Region
55-64Views:74Through a non-representative sample of the small enterprises of the North-Alföld Region, this paper studies the product strategic priorities enabling us to make a detailed typology of small
enterprises. According to the relevant technical literature, the types of small enterprises have typical strategic priorities. Obviously, each group can be characterised with certain strategic
priorities, which are sharply distinct from each other. These priority features are shown in the form of characteristic features in the enterprise’s marketing activities, market behaviour (product policy, innovation attitude, technology, price policy). This paper studies the enterprises of the sample through the role of production technology, material quality, low price and branding endeavours affecting product positioning. -
Preliminary studies to evaluate the use of spectral data in monitoring of apple orchard parameters
37-41Views:114The introduction/application of precision agricultural technologies has more important role in various fruit growing sectors among others apple growing. Remote sensing methods can detect electromagnetic waves where the green colour of the leaf is responsible for the chlorophyll content. The absorption of chlorophyll is in the wavelength range of 450–670 nm. Samples of apple tree leaves were taken on a weekly basis from the apple orchard at Horticultural Unit of Pallag on University of Debrecen in 2019 summer. Our studies were performed on 2 cultivars (Early Gold, Golden Reinders) and the samples were processed using 2 methodologies: a non-destructive spectral method and spectrophotometric method chlorophyll and carotenoid contents were calculated, which were created into some groups and compared with the spectral values. When the plant begins to lose strong green colour and turns yellow spectral measurements show that chlorophyll content decreases as the proportion of chlorophyll-carotenoid in the plant changes. In case of grouping into intervals, it can be observed that as the chlorophyll content increases the reflectance value decreases continuously due to the strong absorption. Based on the results, close relationship between the pigments can be detected.
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Studies of Expression of Peptaibol Synthetase of Trichoderma reesei
188-190Views:130Because of the potential importance of peptaibols in the biological control of plant diseases, a transgenic, a T. reesei strain carrying a tex1-promoter: goxA fusion plasmid was constructed for furthur studies. The peptaibol synthetase gene (which is highly similar to T. virens tex1) was identified in the genome sequence of T. reesei. A 900 bp 5’ upstream noncoding fragment, presumed to include the promoter region of tex1, was cloned into the pSJ3 plasmid (which contains the Aspergillus niger goxA gene encoding glucose oxidase). Finally, we transformed T. reesei with the tex1-promoter: goxA fusion containing pSJ3 plasmid.
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Molecular studies on Cryphonectria parasitica isolates from Carpathian-basin
76-80Views:96Chryphonectria parasitica, the casual agent of chestnut blight, is one of the most important fungal pathogens of chestnut (Castanea spp.) in Europe and Hungary. In this study we analyzed the diversity of 14 Cryphonectria parasitica strains isolated from different location of Carpathian-Basin. For the analyses we used the partial sequences of the translation elongation coding gene, tef1. Our results showed that the tef1 gene, contrary to other fungal species, is not suitable for the molecular analyses of C. parasitica. In the future, for the molecular studies of C. parasitica, we need to use other molecular markers like microsatellites.
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The effect of season on the microbiological status of raw milk
95-99Views:136Many factors can influence the microbiological quality of raw cow’s milk. In this study, our aim was to determine whether there was any difference between the microbiological statuses of milk produced in different seasons. Samples were collected and analysed from five dairy farms in Hajdú-Bihar County, from February to November in 2019. During our studies, total plate count (TPC), coliform count and Staphylococcus aureus count of raw cow’s milk samples were determined.
There was no significant difference (P>0.05) between the mean TPC values detected in the milk collected in winter and autumn, but that values were significantly (P<0.05) lower than in the milk samples collected in spring and summer. Similarly to the TPC, in the case of coliform bacteria the lowest mean colony count was detected in the samples collected in winter. The difference was significant (P<0.05), compared to the values observed in the samples collected in summer. S. aureus was detected in bulk milk of only two farms in excess of 1.0 log10 cfu/ml. Also in case of S. aureus, there was a significant difference (P<0.05) between the values observed in the samples collected in winter and in summer. Samples from spring and summer contained the highest amount of S. aureus.
Based on the results of our studies, in the case of almost all farms the mean TPC, coliform and S. aureus counts were lower in the samples collected in winter, than in the samples collected in summer. The fact that the samples collected in winter contained the lowest amount of colonies could be attributed to the inhibition of growth of mesophilic microorganisms below 8 °C. Furthermore, the fact that we observed the highest colony counts in samples collected in summer, can be related to the heat stress of cows during the summer due to unfavorable weather conditions (high temperature and humidity).
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Impact of precision irrigation on the unit income of maize production
157-162Views:39The study of the economic/economic impact of precision farming should be a priority area in digital agriculture, as the results, profitability, and efficiency indicators can have a significant decision-support effect on the development of both the agronomic and the technical regions of individual farms both in the longer and shorter term. Individual firms, companies, farmers, and family farms quantify the effectiveness of their farming processes. The modern age offers the possibility of digitally recording all the elements of farming technology, making it possible to analyse the cost-effectiveness of a farm more effectively and, in some cases, to carry out more detailed analyses. Nevertheless, the number of farms demonstrating their profitability with such precise economic calculations is still minimal.
Our analyses were conducted on a 56,02 ha field of Balogh Farm-Tépe Ltd. The agricultural operations carried out were fully documented so that the inputs (seeds, fertilisers, pesticides, crop enhancers) were recorded in coordinates and kind, as well as the specific yields, grain moisture data, irrigation norms, and irrigation rotations. At the same time, the company's owner provided the data's monetary value. The main econometric indicators (yield, production value, cost of production, income, cost price) related to the evaluation of the enterprise management were evaluated along with the spatial data in the irrigated and non-irrigated tables. Our calculations show that a given year's climatic and market characteristics fundamentally determine the cost and income relations of a plot of land (and thus of an entire farm). In addition to additional inputs, introducing some elements of precision farming and intensification and increasing yields improves yield security and allows for excellent yield stability.
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Studies on the Suitability of Different Mould Media Compositions for the Mycological Evaluation of Hay Samples
34-38Views:74t evaluating mould contamination of hay samples in more than the half of the cases Mucor spp. (and Trichoderma spp. in a smaller extent) overgrew slower developing moulds, spoiling the assessment of total mould CFU and the detection of fastidious organisms, among others the toxinogenic Fusaria. In parallel microbiological evaluation of hay samples comparing to the ISO 7954 medium as reference a) the overgrowth inhibiting effect of Rose Bengal (ISO 7954-RB); b) the combined inhibiting effect of Rose Bengal and Dichloran (dichloro-mononitroaniline) in DRBC; c) the inhibition of Dichlorane in ISO 7954 (ISO 7954-DC); d) the lowering of nutrient levels to 1/4 (1/4 N ISO 7954, 1/4 N-DRBC and 1/4 N-DC); e) the inhibiting effect of dinitro – salicylic – acid (DSA), a reducing sugar binding compound, as a potential growth inhibitor (RBC-DSA) were studied. The results showed, that 1) MSZ-ISO 7954 medium codified by the official method was unsuitable for the detemination of mould count and for the detection of toxinogic spp. in hay samples. In half of the cases the overgrowth of Mucor has spoiled CFU enumeration and recognition of toxinogenic moulds; 2) Inhibitor supplemented DRBC medium (King et al., 1979) enabled early CFU enumeration by uniforming colony sizes and by efficient suppression of Mucor, but the pink background colour of the medium was disturbing the observation of tints of conidia, which were characteristical to toxinogenic moulds like Fusaria. The hypha-staining property of Rose Bengal did not prove very important; 3) According to the recent stage of our studies, the ISO 7954-Dichloran combination can be recommended for the mycological evaluation of hays and dried roughages. CFU can be enumerated early, Mucor suppressed in the same extent as with DRBC and colours are easily observable; 4) 2-4-Dinitrosalicylic-acid (DSA) proved unsuitable as inhibitor, for its poor efficiency and for its intense yellow background coulour.
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Phylogenetic studies of soybean pathogen Phoma species by Bayesian analysis
53-61Views:137We carried out phylogenetic study analyzing sequences of genetic markers in the taxonomy of Phoma and Phoma-like fungi. Different species of Phoma and Phoma-like fungi occurring on soybean (Phoma pinodella, Phoma sojicola, Phyllosticta sojicola, Phoma exigua var. exigua) are difficult to identy because of their high morphological and symptomatic similarities.
Twenty-two isolates of nine different Phoma species were obtained from reference culture collections. Seven of them were isolated from soybean, the others were collected from different hosts.
The Phoma isolates were firstly characterised by morphologically, and then we employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1), ITS region, as well as β-tubulin partial sequences as potential genetic markers to infer
phylogenetic relationships among different Phoma species..Finally, their ITS and tef1 sequences were sequenced and analysed by Bayesian approaches.
According to phylogenetic trees inferred by Bayesian analysis of tef1, ITS and β-tubulin sequences, different Phoma species can be separated proving that these phylogenetic markers are well suited for phylogenetic studies of Phoma species. However, the phylogenetic tree does not support the traditional Phoma sections based on morphological characterization.
Bayesian analyses of the three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The molecular data provide evidence for reclassification of formerly mentioned soybean pathogens. -
Studies of Cryphonectria parasitica (MURR.) BARR subpopulations on Quercus petraea in Ukraine
70-75Views:122Chryphonectria parasitica (Murr.) Barr, the casual agent of chestnut blight disease, which is one of the most important fungal pathogens of chestnut (Castanea sativa). The disease seriously affected the chestnut in Northern-America and in Europe as well. It is important to mention that the pathogen does not only infect the chestnut but oak species (Quercus spp.) also. In the Carpathian-Basin, the chestnut is endemic in the Mecsek mountains, in Zala, in Somogy counties but it also can be found in the Danube-Bend. In the Carpathian-Basin (outside Hungary) the chestnut is found in Slovakia, Ukraine, Romania. In our study bark samples infected by Cryphonectria parasitica were collected from Bobovyshche, Serednje and Rostovjatica (Ukraine). The rate of infected chestnut tree were higher than 90% around Bobovyshche and beside chestnut, the symptoms were detected on oak trees as well. We collected bark samples from chestnut and oak as well and then we isolated the pathogen Cryphonectria parasitica in the lab of University of Debrecen. Symptomatological observations, laboratory examinations on fungus morphology, as well as comparisons of ITS sequency homology were made and approved that the causal agent of new disease was Cryphonectria parasitica. Our results proved that the Cryphonectria. parasitica infects oak trees beside chestnut in the Carpathian-Basin. Further studies are needed to determine the VCG (Vegatative Compatibility Group) group of the Cryphonectria parasitica found on oak trees.
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Recent data about the fish consumption of great Cormorant in the area of Hortobágy Fish Farm Co
75-80Views:217The cormorant (Phalacrocorax carbo) is a bird species that nests sporadically but in colonies, besides larger fishponds and rivers in Hungary. The number of its individuals has been increasing during the last two decades. The species eats solely fish, therefore it can cause serious depredation of the fish stocks in fishponds of intensive system and after the freezing of the ponds, in larger rivers, which are not yet frozen. The aim of our research was to reveal the damages the birds can cause on the studied areas and the extent of the losses the Hortobágy Fish Farm Co. has to realize.
Our studies were carried out between April 2012 and November 2014. During cleaning, the investigation of the craw contents of the birds and their biometric studies were conducted. The results revealed the diverse food base of the species. In the samples, we have determined 379 fish individuals; in 368 cases, the exact fish species were determined, too. Significant differences were found between the consumed fish species rations of the units (P=0.05% beside). We have also found significant difference in the fish consumptions of adult males and females.
Under the ever harder fish production conditions, the presence and thus the permanent predation of the birds affect the fishermen. In favour of maintaining ecological balance as well as to hold the income of the fish farmers, elaboration of adequate protective and preventive methods will be needed in near future.
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Studies on Ostrich (Struthio Camelus) - Review
15-22Views:1013Ostrich has been reared in Hungary for decades, but we have limited information on this species. The aim of this review was to provide a concise description of the ostrich (Struthio camelus) based on international studies. We were to investigate some of the most relevant parameters, such as egg, meat and skin production. In this critical review we managed to sum up the most significant features and productivity parameters of ostrich and effecting factors. So as to make an accurate description of the species we have to know its morphological, behavioural and physiological characteristics. Ostrich is a very special bird with special nutritional and environmental requirements. Ostrich products, regarding egg, meat and skin are considered luxury products not only in Hungary but also abroad. Though egg has a significant nutritional value, it is mainly used for hatching chicks. In Europe we can expect roughly 40–50 eggs from a hen yearly. Ostrich has a lean meat with high protein and low fat and sodium content, moreover it is rich in minerals and polyunsaturated fatty acids. Having these advantageous qualities, ostrich meat should be part of our healthy diet. Skin production is not important in our country, but there is a special demand for high quality ostrich leather globally. In the future our aim is to conduct a research on some of the parameters. As low production rate and embryo mortality is a great deal of problem in ostrich industry, we need to investigate the incubation environment. There is an apparent disagreement among researchers on optimal slaughter age. This is also an area for further investigation. The value of skin and effecting factors on our climate as well as adaptability of the species are also worth for further investigation together with the nutrition what differs from other ostrich breeding countries. Nowadays, there is a great emphasis placed on animal welfare too.
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Public opinion on the settlements’ environment and the use of renewable energy sources in the Micro-region of Gyöngyös
267-272Views:66This paper intends to give an overview on some results of our studies carried out on the public opinion of the above aspects (settlements’
comfort level and the use of renewable energy sources) directly or indirectly linked to the topic of climate change and possible adaptations.
These studies were carried out by applying questionnaire survey in the (altogether 25) settlements of the Gyöngyös Micro-region.
Knowledge on general human perceptions related to climate change and its impacts is considered to be important as it should advance the
elaboration of adaptation and applied scientific works. They are highly required as rural areas, due to their closer relationship (dependence)
to the physical environment, are more susceptible to changes resultant from the climate change with their possibilities to adaptation also
being impeded.
The beauty of environment plays an important role in the settlements studied within the micro-region with more than two third of pollees
opting the environment as a factor they most proud of, especially among those residing in the villages for more than 10 years. It can be
presumed that any change in this environment would greatly impact their everyday life and emotional relationship to their home villages. The
overall picture after analysing the public opinion on the use of renewable energy source (as possible way of adaptation to the impacts of
climate change) is rather controversial. Even basic knowledge and information are limited regarding the relevant technologies calling
attention to the importance of raising environmental awareness and providing full information seems to be essential and should be
implemented, through the media and education.