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Investigations of Thrips tabaci and Aeolothrips intermedius population dynamics in tobacco plantations
121-127Views:185Studies were conducted between 2015 and 2017 with yellow sticky traps in seven tobacco plantations. The purpose was to determine when and in what numbers onion thrips individuals can settle into tobacco plantations. The primary objective of the study was to determine the proper timing of chemical treatments, furthermore, the determination of crucial factors that can influence the population dynamics of Thrips tabaci.
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Review on the fatty acid profile and free fatty acid of common carp (Cyprinus carpio)
99-105Views:83Carp or ponty in Hungarian, is considered commercial freshwater fish, which is an adaptable species in both wild and cultured conditions. Carp has high nutritional value content, favorable taste, it is rich in protein, and low in saturated fat. The nutritional content in fish is composed of many chemical constituents and influenced by many factors. One of the components that its content may be different due to internal and external factors is fatty acids, which may vary depending on endogenous and exogenous factors. The endogenous or internal factors include the genetic, size, sexual maturity, and life cycle phase. While microclimate, water quality, quality of food or diet habit, and the amount of available food or starvation are considered as exogenous or environmental factors. Freshwater fish has the ability to convert essential fatty acid into long chain polyunsaturated fatty acid like AA, EPA, and DHA. Most results showed that palmitic acid and oleic acid were the dominant SFA and MUFA in carp both for wild and farmed carp in all seasons. The PUFA for wild carp was mainly dominated by DHA, while on farmed carp by LA. It confirmed that high LA content in farmed carp was related to the diet habit. The amount of lipid and FA were changed in line with the season. Even the statistical analysis showed no significant difference, but some studies showed a contrasting result. Moreover, most obtained results acknowledged that FA tends to decrease during the spawning period. The amount and composition of FA were affected by the total lipid content. The lipid must be broken down into simpler compounds such as FA or FFA for the metabolism of fish. The result of metabolism then transported into the utilising tissue and used as energy.
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Anti-Oxidants in Agriculture
195-200Views:83Today’s programmes aiming at enhancement of fruit and vegetable consumption have been intensified. In the unanimous view of experts, different health problems, such as those of the immune system, inflammations, and even certain cancerous diseases can be prevented and/or cured with regular consumption of fresh (raw) fruit and vegetables. It is well-known fact that among the biologically valuable components, antioxidant compounds – C- and E vitamins, as well as carotinoides – play an important role. In this field, Hungary can expect success in the future since it has excellent plant genetic stock. Regretfully, national data banks regarding cultivation technology, cultivation areas or varieties for fruits and vegetables and their antioxidant contents do not exist in Hungary. Nevertheless, in connection with the so-called “Hungaricums” its existence would be of urgent necessity. Such excellent Hungarian products are – among others – a lot of sour cherry varietiles, the Szeged green pepper and the Makó onion. They enjoy high priority as “Hungaricums” even in the European Union and such activities that support these kinds of products should be enhanced by intense and consequent research work, which may prove their role as functional foods. Presently’ the USA leads in the research of antioxidant compounds of sour cherry, and so far 17 of these compounds have been found partly in Hungarian varieties. Similar research on green, and ‘pritamin’ peppers have not gone so far since they were limited only for seasoning paprika. In Hungary, studies on onion and garlic have not been performed. It should be mentioned that due to the continental climate, these products may be cultivated, consumed or processed only in a limited period. Taking this into consideration, processing and conserving methods are needed which make the consumption of these fruit and vegetables as functional food possible year-round. Scientific establishment of this set of questions is of current concern, because consumption of these products could have an important role in improving the health status of the Hungarian population in the future.
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The Role of Schengen in the Development of Peripheral Borderland Regions
155-158Views:63This study aims to uncover the role of the Schengen borders of the European Union in rural and settlement development. Schengen integration applies certain restrictions at the external border-crossings, so the filtering role is to be taken into consideration. In addition to the disappearance of borders in the globalising economic area, the strict Schengen rules further burden the development of cross-border interactions, bringing about less frequent border crossings. Moreover, the economic integration of the affected borderlands would remain sluggish. The author points to the fact that the dynamics of a border interaction system should include a Schengen border degree between the interdependent and integrated borderland levels. Consequently, the Schengen borderlands should be in the focus of further border studies.
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Theoretical and comparative study regarding the Galerkin-Vlasov method use for study the free vibrations of wood flat plate
223-228Views:49As for the historic record of the problem related to the study of flat plates, the first results were out for publishing at the end of the 18th century, the beginning of the 19th century, having Chladni E, Strehlke, Konig, R, Tanaka S, Rayleigh L, Ritz W and later on Gontkevich V, Timoshenko S, Leissa as pioneers. Each of the above mentioned authors have had significant contributions regarding the development of methods in order to solve the plates and establish some rigurous solutions of their differential equations of equilibrium.
The making of constructions, machines and different high-perfomance appliances, whose functioning should take place in safety conditions, have required theoretical studies of rich complexity, as well as practical experiments, within which the problem of their free and forced vibrations represent an important category in the respective theme of research. -
Preparatory studies for modelling production on protected grasslands
64-69Views:146There is a mutual dependence between nature conservation activity and agriculture in Hungary, as the management of the protected areas cannot be achieved without ecological farming methods. Moreover, viable economic activity can be only imagined through the harmonization of agricultural and nature conservation interests. From a nature conservation point of view, grass management systems play the greatest role in domestic agricultural systems. Yet, due to the prohibition of certain management methods and the spatial and temporal restraints on grazing, nature conservation activities have priority on protected grasslands. While nature conservation activity is still of prominent importance, it is not equally suitable for the economical management of protected grasslands per se. With our examinations, we would like to emphasize the common interests of these mutually dependent activities and to promote bilateral cooperation. Our aim is to model the production of grass on the great pastureland of Hajdúbagos. Potential grass production levels are easily calculable with a computer model based on data collected through a series of test harvests, as well as by factoring in changing climatic factors and by simulating the effects of grazing animal species and stocks. This model is not only useful for determining the optimal number of the grazing animal stock and grazing method, and therefore the most suitable management strategy, but it also supports local farmers to be able to plan their activities. In this way, both nature conservation and economic aims can be easily harmonized, which would be an important factor for the sustainable development of rural areas.
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Application of advanced environmental assessment methods in orchard management
221-225Views:114Our reseaches were carried out in apple and pear orchards at Farm and Regional Research Institute in Pallag of the University of Debrecen and Pear Gene Reservoir in Újfehértó. Aim of this study is to interpret and analyse field studies with the aim of a GIS based database. Furthermore, beside field measurements, airborne and field hyperspectraldatacollection and analysis were also made to facilitate special watermanagement and irrigation related surveys. The integration of unified, geoinformatics systems with high spatial resolution and calibrated airborne hyperspectral data are appropriate tool for decision support systems, which support the continuous update and actualization of the changing cropping data, the analysis of cropping results in a unified complex data system, the acquiring of agro environmental subsidies, the establishment of monitoring system, and the optimization of irrigated fruit production.
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Mitochondrial DNA-based diversity study of Hungarian brown hares (Lepus europaeus Pallas 1778)
23-29Views:131The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.
The aim of our large scale long term ongoing study is to fill this gap of information on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.
This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.
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Morphological and leaf anatomical studies on Cleistogenes Serotina (L.) Keng (Poaceae)
5-13Views:76The grass species Cleistogenes serotina (L.) Keng, generally found in the mountainous region in Hungary, has been observed in the lowlands of the Great Hungarian Plain in the last few decades. Since its first recent mention in sand habitat as well it, has been found in several locations in the interfluve between the Danube and the Tisza. In certain, sandy grasslands, the invasion of this species is considerable (e.g. near Fülöpháza the area invaded by Cleistogenes increased almost fourfold within six years). The success of invasion on new habitats is related to changes in the
characteristics of plants comparatively studied on mountain and sand populations.
We studied blades of leaves on different levels (1st, 5th and 10th) and from different biotops. Cuttings were made, with a thickness of 10-12 μm, at the middle of the blades. The cross sections were observed using a light microscope. Digitised images (DigitPlan) and survey data were arranged into a database.
The leaf structure is characterised by the kranz anatomy, and a double layered bundle sheath, which is typical of the aspartate type of C4 grasses. There were significant differences only in cross section area and the number of bundles for various ages of the leaves. There were more bundles in the older leaves than in the younger ones. We did not find any difference in the ratio of tissues among leaves of different ages and sources. According to our study, the ratio of leaf tissues characteristic to species was neither significantly influenced by the biotop, nor by the age of the leaves. However, there is a difference in tissues size. Summarising the results, the species can be considered „only” as ecotypes on the
different studied areas.
Our new result is that kranz anatomy can also be found in the leaf-sheath.
This research was supported by the Hungarian Scientific Fund (OTKA), grant No. T016511 and T026346. -
Studies of the effects of N fertilizers and Microbion UNC biofertilizer on microelement content of horseradish (Armoracia macrocarpa)
41-45Views:115A field experiment on calcareous chernozem soil was performed to study the effects of different N and bacterial fertilizers on the nutrient content of horseradish (Armoracia macrocarpa). In the experiment the trials were arranged in a randomized block design with three replications, applying three levels of NH4NO3 and different N fertilizers, namely ammonium-nitrate, urea and calcium-nitrate, with or without application of Microbion UNC biofertilizer.
In the present paper the changes and distribution of manganese, zinc and copper contents of the horseradish plant are summarized by the
effect of different treatments.
The Mn content of leaves were higher in all cases than those of roots, but Zn mainly accumulated in the roots. The distribution of copper within the horseradish plant was more equalized than that of Zn and Mn. Different N fertilizers and increasing doses of ammonium-nitrate had effects mainly on the microelement contents of leaves. The highest Mn contents of plant were measured in treatments of Ca(NO3)2 and Ca(NO3)2+Microbion. The lowest ammonium nitrate dose (N1) decreased the Mn content of leaves compared to control, but further doses
(N2, N3) did not alter these values any longer. Microbion UNC biofertilizer did not have any effect on the Mn content of roots, but we measured higher Mn in leaves in some combined treatments. Ca(NO3)2 increased the zinc content in leaves and roots in a noticable manner. With the increasing of NH4NO3 doses, the Zn content of leaves and roots augmented significantly. Neither N fertilizers (or the increasing doses of NH4NO3) nor the biofertilizer application influenced the Cu content of horseradish plant.
N fertilizers had higher effects on the microelement content of horseradish, the biofertilizer’s effect was smaller and was not the same in every treatment. -
The significance of biological bases in maize production
61-65Views:159The comparative trial has been set up in the Demonstration Garden of the Institute of Crop Sciences of the University of Debrecen, Centre for Agricultural and Applied Economic Studies, Faculty of Agricultural and Food Sciences and Environmental Management in 2012, with 24 hybrids with different genetic characteristics and growing periods. The soil of the trial is lime-coated chernozem, with a humus layer of 50–70 cm.
The weather of the trial year was quite droughty; the monthly average temperature was 3–4 oC higher than the average of 30 years. High temperature, together with lack of precipitation occurred during the most sensitive phenophases of maize (flowering; fecundation, grain saturation).
The following characteristics have been observed: starting vigour, date of male and female flowering, plant and cob height, dry-down dynamics during maturation and the change of yield composing elements has also been quantified. The yield was recalculated to 14% moisture content grain yield after harvesting.
The beginning of the growing period was advantageous, therefore the analysed hybrids could grow a high (above 300 cm) and strong stem. The yield of the hybrids changed between 10.33 and 11.87 t ha-1, but as a result of the unfavourable climatic extremes, their genetic yield potential prevailed only at a rate of 30–40%. However, moisture content by the time of harvesting was good despite its early date (12th September); it remained under below 14% in most cases. Dry-down was measured on a weekly basis between 14th August and 5th September.
The analysis of the qualitative parameters of the maize hybrids (protein %, oil % and starch %) resulted in significant differences. The most significant difference has been observed in the case of protein content (LSD5%=2.01). Oil content was the most advantageous in the case of hybrids belonging to the mid-late growing group (FAO 400). The X9N655 and 36V74 hybrids had the highest oil content (around 4%), while hybrids P9915 and 37F73 had significantly lower oil content. Starch content was above 70% in the case of every hybrid.
Hybrid selection is highly important in terms of yield and yield security of maize, as well as the application of modern biological fundamentals and hybrid specific technology for the improvement of the level of cultivation technology.
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The queen of the sports with the eyes of the women
25-32Views:196The media plays a very important role in the gender socialization as in the sport selection also, in which the individual is born,. We want to introduce those disciplines through the development of more studies are in athletics not least, belonged to a man's privilege. The emergence of women in a variety of masculine competitions has enabled the track and field sport turned into both gender sport. The masculinity and feminity separation in sport is increasingly disappear and become neutral. In our study, we analyze the changes in women's athletics point of view of history, in some disciplines for women and the availability of representatives assessment of Hungary. We based on Methenys categorization in 1965, and Bem's study, written in 1974, and examined women's branch of track and field sports acknowledgment and acceptance focused on the masculine category.
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Productivity of thinned black locust (Robinia pseudoacacia L.) stands in Hungary: case studies
181-186Views:95Black locust (Robinia pseudoacacia L.) is one of the most important introduced tree species in Hungary, covering more than 24% of the stocked area and providing approximately 20% of the country’s annual timber cut. Consequently, the research and development activities related to the improvement of the growing technology of black locust are also important, especially in mitigating the negative effects of climate change. The aim of this study was to quantify growth and yield, responses of even-aged 12–31–year–old black locust stand to thinning in Hungary. The study has proved that irrespective of the yield class, age and thinning intensity, thinning could not increase effectively the cumulative volume production. As percentage of the control it has changed between +0.6 and -8.4%. On the other hand, it could increase the stand value based on stem quality index by 10–21%.
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Comparison of RAPD and AFLP Analysis in Some Maize (Zea mays L.) Lines and Hybrids
3-7Views:88The use of molecular markers to enhance plant breeding efforts is being widely studied. DNA-based fingerprinting technologies (RAPD and AFLP) have proven useful in genetic similarity studies. We estimated different maize (Zea mays L.) inbred lines and hybrids originated from mutant ones based on their genetic differences.
We carried out RAPD analysis with different primers and the 707 (CCCAACACCC) and 792 (CAACCCACAC) primers with 50% similarities provided quite good DNA fragments. By applying the DNA based-AFLP technique, we had very dense DNA fingerprinting. We differentiated 15-32 polymorphic bands, the highest number of bands were found in P-T/H-CA (32). AFLP seems to be the more efficient method of comparing genetic similarities/differences among different genotypes. -
Testing a biological active plant extract’s antifungal effect against soil fungi
247-252Views:95In Hungary today is about 5 million hectares of agricultural land contaminated with ragweed. The ragweed problem a year is about 60 billion HUF to be paid, of which 30 billion are used to reduce the agricultural damage. Experiments with ragweed pollen has mainly been carried out in connection with terms of allergy. The other biochemical experiments and studies with this plant, have so far been the scientific horizons of public life, boosted the edge. We wanted to demonstrate that the ragweed, which is a weed, containsbiological active (for example: antifungal) compounds. For our experiments in the previous cycle of flowering, plants were collected manually, with its roots and with each plant part. The extraction of the substance from dry plant – meal was carried out using appropriate solvents. The biological activity of ragweed-extracts were tested against fungi isolated from soils and meadow with different mode of cultivation. Our results suggest that ragweed contains biologically active substances, which inhibit the growth of fungi, depending on the concentration of active ingredients of the plant.
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The toxicity of different selenium forms and compounds – Review
33-38Views:1800Selenium (Se) is an unusual metalloid of considerable interest from both a toxicological and a nutritional perspective, with a very narrow safe range of intake. Although there are many reports about its detoxification properties, toxicity aspects of it have also been tracked for several decades. Lots of studies demonstrated that low Se is an efficacious avail whereas high Se can induce toxicity and the significant toxicity of selenium emphasizes the need to assess the health risk of various selenocompounds as nutritional supplements. The toxicity of different forms and compounds of selenium is also summarized in this review.
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The effect of fertilization and plant protection in sunflower (Helianthus annuus L.) production
57-63Views:221Sunflower is the most important oil crop in Europe which is grown on the biggest area of all the oil crops. The area of producing sunflowers in Hungary was changing to 524–704 thousand hectares in the past decade in comparison with approximately 100 thousand hectares in the 1970’s. In our experiment different sunflower hybrids were examined. The doses of fertilizers were the following in 2017 and 2018: 0–30–90–150 kg ha-1 N, 0-50-90-90 kg ha-1 P2O5 and 0–70–110–110 kg ha-1 K2O. Three different treatments of plant protection were used in the experiment to protect them from fungal infections. A rise in the dosage of nitrogen resulted in increasing infection. The biggest fungal infection was identified in 150 kg ha-1 N, 90 kg ha-1 P2O5 and 110 kg ha-1 K2O treatment. The largest infection was measured on plots without plant protection at the highest N doses, both in leaf and disc diseases. We measured the highest 41.25% in 2017 and 53.1% in 2018 Diaporthe helianthi infection, and 24.5% in 2017 and 25.5 % in 2018 Sclerotinia sclerotiorum disc infection in these plots. kg ha-1
Average yield was changing between 2.96–4.67 t ha-1 in 2017/2018. The lowest yield was obtained in the absolute control plot (without plant protection and without nutrient) in both years, which was 2.96 t ha-1 in 2017 and 3.14 t ha-1. The yields increased due to growing nutrient supply and excellent plant protection. The highest yields were 3.67 t ha-1 in 2017 and 4.67 t ha-1 in 2018 (150 kg ha-1 N, 90 kg ha-1 P2O5 and 110 kg K2O active substance). Purpose of the experiment at analysing the impact of different treatments on the profitability of sunflower production.
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Physiological traits and yield of three soybean (Glycine max (L.) Merr.) genotypes as affected by water deficiency
11-15Views:181Soybean is a very important legume; it has the highest protein content, and it is a very important source of vegetable oil. Soybean is droughtsusceptible, and drought is one of the major abiotic stresses that has been increasing over the past decades as a result of the global climatic changes. To evaluate the influence of drought stress, three soybean genotypes were grown under rainfed conditions, and compared to irrigated controls. The obtained results showed that the chlorophyll content, leaf area index and plant height decreased under drought stress conditions, which led to noticeable and sometimes significant yield reduction. Our results suggest more specific studies on the physiological changes of the local soybean genotypes under drought stress to better select the adopted ones.
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Comparative study of newly-bred black locust clones with regard to photosynthetic rate and water use efficiency: early evaluation
5-10Views:200Black locust (Robinia pseudoacacia L.) is one of the most important tree species in Hungary, due to its positive economic impacts. Research to increase its yield, improve its stem quality and enhance its drought tolerance has been ongoing since the 1960s. Of the current research works in this field, the clone trial of the Forest Research Institute, University of Sopron, established in 2020 in the Nyírség region, is worth highlighting. In this experiment 4 newly-bred clones and a state-approved black locust cultivar ('Üllői') are being tested. In the summer of 2022, ‘on site’ measurements of assimilation parameters – net assimilation (An), transpiration (Tr) – were carried out using the LI-6800 portable photosynthesis system. From the data obtained, the water use efficiency (WUE) was calculated. The results of the statistical analysis (Kruskal-Wallis H test) have shown significant differences (p < 0.05) between the clones for all three parameters (An, Tr, WUE) tested. The NK2 clone has performed the highest value for all the parameters studied. However, no significant differences were found between clones NK2 and PL040 for Tr or between NK2 and control ('Üllői') for WUE. Studies of this kind contribute to the improvement of black locust growing through the production and selection of cultivars, which are relatively resistant to the negative effects (drought) of climate change.
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Molecular Biological Approach of Crocus sativus L. and its Allies
112-115Views:120The hay saffron (Crocus sativus L.) is a sterile triploid plant, known in human culture only, with no fertile seeds produced. The origin of saffron is still a mist, however it is assumed to be an autopoliploid mutant or a hybrid. The recent classification and most of the former taxonomic publications define C. sativus to be derived from C. cartwrightianus, a wild species. Because of the sterility of hay saffron it seemed to be reasonable to apply molecular biological methods to complete classical taxonomic studies in examining its relations. The DNA polymorphism based AFLP method has confirmed the close relationship between these species.
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The effect of the “Transcarpathia” Free Economic Belt on the investment activities of the region and its development of foreign trade relations
149-153Views:51The article analyses the figures of foreign investments and their territorial distribution and their change due to the impact of the “Transcarpathia” Free Economic Belt. The article studies the consequences of the liquidation of the “Transcarpathia” Free Economic Belt and the issue of restoring allowances that formerly existed on its territory.
The Free Economic Belt that was founded in 1998 and its customs and tax allowances invoked the settlement of affiliated firms of several world corporations, like the “Eurocar”, the “Yadzaki”, or the “Jabil”. However in 2005 the government of that time repealed all allowances granted by the “Transcarpathia” Free Economic Belt, and due to this the increasing dynamics of the amount of foreign stocks which was penetrating into the economy of Transcarpathia started to decrease. Many companies halted their work which went alongside with closing-down several thousands of workplaces. The working “Transcarpathia” Free Economic Belt had a positive effect on the social and economic indices of the region, so discussions regarding its restoration have already been started between the president of Ukraine and the governor of the region. -
Analysis of the genetic origin of European hare (Lepus europaeus) (technical literature review)
49-54Views:142Fossil datas showed that the lagomorphs appeared in Asia during the later Eocene and leporids separated from ochotonids during the Oligocen or Upper Eocene. During the Pleistocene, climatic changes induced major shifts in species distributions, forcing them to refugia.
In Europe, the continuous oscillations led to the production of greater subspecific and specific diversity in the southern peninsulas (Iberia, Italy, Balkan) which were the main refugial areas for other species as well. Evolution, phylogeny and population genetics of the Lagomorpha species are still poorly known and the taxonomic distinction is still unclear for some species. Conservation genetics is the discipline which use genetic techniques and analysis methods to preserve species and dynamics. Usually it is working together with population genetics what role is to investigate the origin, the maintenance, the organization and the causes of genetic variation between natural populations. In the present study I reviewed the results of origin and phylogenetic studies of Brown hare populations from the last decades. -
Studies of the influences of different N fertilizers and Microbion UNC bacterial fertilizer on the nutrient content of soil
134-140Views:60A field experiment was conducted to examine the effects of different nitrogen fertilizers in combination with bacterial fertilizer on
nutrient uptake of horseradish and plant available nutrients of the soil. Three different N fertilizers, ammonium-nitrate, urea and calciumnitrate
(116 kg ha-1 N) in combination with Microbion UNC bacterial fertilizer (2 kg ha-1) were applied as treatments in a randomized
complete block design in three replications. In this paper we presented the results of soil measurements. The soil of the experimental area
was chernozem with medium sufficiency level of N and P and poor level of K.
Our main results:
The amount of 0.01M CaCl2 soluble inorganic nitrogen fractions, NO3
--N and NH4
+-N and also the quantity of soluble organic-N were
almost the same in the soil. N fertilizers significantly increased all the soluble N fractions. The amount of NO3
--N increased to the greatest
extent and the increase of organic N was the slightest. We measured the largest CaCl2 soluble NO3
- -N and total-N contents in the plots
treated with ammonium-nitrate, the largest NH4
+-N in the plots treated with calcium-nitrate and the largest organic-N fraction in plots
treated with urea.
Bacterial inoculation also increased both soluble inorganic nitrogen forms and also total-N content of soil compared to the control. In
the case of combined (artificial and bacterial fertilizer) treatments we measured lower NO3
--N, organic-N and total-N compared to the
values of plots having only nitrogen fertilizer treatments. On the contrary in the plots with combined treatments the CaCl2 soluble NH4
+-N
content of soil in more cases were higher than that of values with artificial fertilizer treatment.
As a function of calcium-nitrate application increased AL-P2O5 and AL-K2O values were measured compared to control. Microbion
UNC supplement of calcium nitrate yielded also increase in AL-P2O5 and AL-K2O values, till then supplement of ammonium-nitrate fertilizer
yielded a decrease in these values compared to the control.
All nitrogen fertilizers resulted in a significant decrease in AL-Mg content of soil compared to the control. Nevertheless bacterial
fertilizer increased AL-Mg values in any cases. -
Comparative analysis of Staphylococcus aureus strains by molecular microbiology methods
34-39Views:108Staphylococcus aureus is a very important pathogen for dairy farms and milk processing plants. Subclinical mastitis is often caused by this species, and it can contaminate bulk tank milk when milking cows are suffering from mastitis. Additionally, thermostable enterotoxins (SE) produced by some types of this bacterium can cause food poisoning.
The aim of our research was to examine the number of S. aureus in bulk tank milk in two dairy farms and the enterotoxin-producing ability, genetic relation (pulsotype) and antibiotic resistance of S. aureus strains from different sources (bulk tank milk, udder quarter milk and environment).
The results show that the mean number of S. aureus of bulk tank milk of two farms significantly differed (P<0.05). Fourteen isolates were selected for further molecular genetic studies (five isolates were from bulk tank milk and nine isolates were from udder quarter milk). S. aureus was not recovered from the environmental samples. Three of the fourteen isolates (21.4%) tested by multiplex PCR were positive for SE genes. Two isolates carried one gene (seb) and one isolate carried two genes (seg and sei). The fourteen strains were classified into three pulsotypes and two subtypes at 86% similarity level. Isolates from bulk tank milk (n=5), were divided into 2 pulsotypes (A, C) and one subtype (C1). The isolates from udder quarter milk (n=9) belonged to three different pulsotypes (A, B, C) and two subtypes (A1, C1). The distribution of pulsotypes in the present study revealed genetic relationship between S. aureus isolated from udder quarter milk and bulk tank milk. This could be explained by the fact that in farms with a high number of infected cows, these cows could represent the main source of contamination. The results of the antibiotic resistance investigations show, that all strains were susceptible to methicillin, cefoxitin, lincomycin, tetracycline, erythromycin and sulfamethoxazole/trimethoprim. Thirteen out of fourteen strains were resistant to penicillin (A and C pulsotypes, A1 and C1 subtypes) and just one isolate was susceptible (B pulsotype) to all antibiotics tested. -
Different soil fertility conditions depending on different land use methods
169-172Views:82In precision nutrient management the most important aspect is adaptation but we should consider the possibility of the long-term improvement of soil fertility within the less fertile landscape zones. This possibility can be evaluated principally by long-term field experiments, which are running on similar soil types. The results of these field experiments can indicate that which soil fertility status should be attained. Some more important soil fertility data, (such as pH, P-, K- and soil organic matter (SOM) content) of a long-term field experiment with increasing farmyard manure(FYM) doses or equivalent NPK fertilizers, set up on an Eutric cambisol, are presented. The yieldincreasing capacity of FYM doses was only 82%, as compared to the equivalent amount of mineral NPK, but long-term FYM treatments resulted in 10% higher SOM content than that of equivalent NPK
fertilizer doses. The studies indicate that SOM content is a function of local climate and clay content of the soil, and neither long-term high FYM doses can increase SOM content steadily above a supposed steady-state value. However we have to make efforts to keep the optimum level. The lowest soil reactions developed both with the highest NPK doses and without any fertilization. AL-P2O5 content of soil was increased more by mineral fertilization than by FYM treatments, but in case of AL-K2O content there was no difference between the fertilization variants. However the highest doses of both fertilization variants increased soil nutrient content to an excessive degree. Wecould get very valuable data from the unfertilized control plots as well, where long-term yield data suppose 48 kg ha-1 year-1 air-borne N-input.