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The effects of spraying with onion and garlic extracts on the growth of the plant Anethum graveolens L. and its antioxidant effectiveness
19-24Views:243This experiment was conducted during the 2022–2023 agricultural season in a mixed clay soil at one of the fields of the Faculty of Agriculture, Basra University. The aim of the experiment was to investigate the effect of spraying dill plants (Anethum graveolens L) with aqueous extracts of onion and garlic on their growth. The plants were sprayed with garlic aqueous extract at three concentrations (0, 20, 30 g L-1) and onion aqueous extract at three concentrations (0, 15, 25 g L-1), with three applications at a 15-day interval between each spray. The experiment was designed using a Randomized Complete Block Design (RCBD).
The obtained results showed that the plants sprayed with garlic aqueous extract exhibited significant improvements in most characteristics, except for the fruit content of total dissolved carbohydrates and the yield of fruits from volatile oil. Specifically, the plants sprayed with a concentration of 30 g L-1 outperformed the other two concentrations in terms of plant height (cm), number of branches per plant, and 1000-seed weight for each experimental unit. On the other hand, the plants sprayed with a concentration of 20 g L-1 significantly outperformed in terms of floral inflorescence number per plant and seed yield. Additionally, the plants sprayed with a concentration of 30 g L-1 had the highest chlorophyll content and antioxidant effectiveness of dill leaves (%). However, spraying with garlic extract did not have a significant effect on the fruit content of total dissolved carbohydrates and the yield of fruits from volatile oil.
Regarding the plants sprayed with onion extract, they showed overall superiority in most traits, except for the chlorophyll content of the leaves. Specifically, the plants sprayed with a concentration of 15 g L-1 significantly excelled in plant height, while the plants sprayed with a concentration of 25 g L-1 outperformed in terms of floral inflorescence number per plant and seed yield. The plants sprayed with a concentration of 25 g L-1 also had the highest content of total dissolved carbohydrates in fruits and the highest antioxidant effectiveness of dill leaves and seeds (%). However, the plants sprayed with a concentration of 15 g L-1 had the highest yield of volatile oil. The interaction between the two factors (garlic and onion extracts) had a significant effect on all studied traits, except for the chlorophyll content of the leaves.
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Concentration and Horizontal Integration in Milk Production
60-66Views:160Agriculture in Hungary was characterised by the duality of large-scale farms (co-ops and state farms) and small-scale private enterprises until the beginning of the 1990s. Due to the privatisation and transformation of co-ops farm structure has significantly changed. The transformation of the structure is not considered to be a completed process. Level of concentration and integration on private farms are rising, new co-operation forms are appearing and new types of enterprises and producers’ groups are being formed.
Concentration and integration have great importance in the dairy sector among agricultural enterprises. In recent years, milk production in the European Union has been characterised by concentration, leading to greater competitiveness. Today, most dairy producers belong to different kinds of producers’ organisations as concentrated demand may only be competitive by meeting concentrated supply. In Hungary, the problems of production have been the fragmentation and decrease of the average farm size while, at the same time, concentration has occurred in the processing sector. Establishing producers’ groups may be one of the solutions for improving competitiveness production. -
Does drought stress always negatively affect the yield and quality of soybean in Hungary?
37-40Views:392Globally-fluctuating climate imposed serious abiotic stresses on the agricultural sector, leading to noticeable, and sometimes disastrous, losses in yields and/or quality of crops; however, in certain cases, plants could survive stress with relatively low reductions, and sometimes even with some enhancements as a reaction to changed environment, especially in the case of mild stress. An experiment was conducted in 2017 and 2018 in Debrecen, Hungary to evaluate the mild drought stress influence on the yield and quality of three soybean cultivars. The results showed that both ES Pallador and Pedro cultivars could achieve more yield when subjected to mild drought conditions; however, protein concentration was enhanced in ES Pallador whereas slightly degraded in Pedro under drought. The cultivar Pannonia Kincse followed different trend; both yield and protein concentration were reduced under drought. Oil concentration of the three cultivars did not show significant changes; however, it always followed opposite trend to that of protein concentration. It could be concluded that both ES Pallador and Pedro are recommended to be grown under rain fed conditions in Debrecen, whereas Pannonia Kincse is recommended under irrigation conditions.
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Study regarding the influence of the nPK fertilizers use in long term trial on heavy metals concentration in wheat grains
107-109Views:196The paper presents research results obtained in stationary experiments, carried out at Agricultural Research and Development Station Oradea, regarding the influence of nitrogen, phosphorus and potassium fertilizers, on Cd, Pb, Cu, Zn and Ni concentration on wheat grains.
The minimum concentration of cadmium in wheat grains was recorded in the case of the unfertilized plot N0P0K0, 0.12 mg kg-1, and maximum value 0.22 mg kg-1, was registered at the fertilized plot with N160P80K120, relative difference relative to control was 84.2%.
Lead concentration had the lowest value in the unfertilized plot, the value (0.4 mg kg-1) being under the maximum allowed (1.0 mg kg-1). In case of the fertilized plot with N160P80K120, the lead concentration had the highest value, 0.47 mg kg-1, but even in this case it was located beneath the maximum limit allowed.
In all four systems of fertilization, copper has not exceeded the maximum allowed limit (5 mg kg-1), the values have been comprised between 1.68 mg kg-1 at the unfertilized plot and 2.81 mg kg-1 at the fertilized plot with N160P80K120. In other plots of fertilization copper had concentrations of 1.97 mg kg-1, at the fertilized plot with N80P40K40, and 2.78 at the fertilized plot with N80P80K80.
The lowest concentration of zinc, 26.47 mg kg-1, has been registered in the control N0P0K0. In the other fertilization plots studied, the zinc concentrations had the following values: 27.66 mg kg-1 (N80P40K40), (N80P80K80) 29.87 mg kg-1 and 33.62 mg kg-1 (N160P80K120).
Nickel has registered the lowest value in the unfertilized plot, 3.47 mg kg-1. The fertilized plot with N160P80K120 had the highest value 4.94 mg kg-1, with 42.39% higher compared to the control N0P0K0.
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Responses of pea (Pisum sativum L.) to the rising atmospheric concentration of carbon-dioxide
185-188Views:370The atmospheric concentration of carbon dioxide increases from decade to decade in increasing pace. In 1957, atmospheric carbon dioxide levels were around 315 ppm, while in 2012 it amounted to 394.49 ppm concentration. In parallel, the global temperature is rising,which is projected to average 1.5–4.5 °C. The carbon dioxide concentration is a key factor – in interaction with the light – affects the plant's photosynthesis. Among the various factors significant interactions prevail: environmental factors affect - the growth and the development of plants, leaf area size and composition, the function of the photosynthetic apparatus, the duration of growing season.
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Market competition and concentration in the world market of concentrated apple juice
129-135Views:232In the study, I examined the market competition and concentration in the word market of concentrated apple juice. I used RCA index to determine comparative advantages In the world China possesses the strongest comparative advantages. Besides China, Poland, Hungary, Chile, Ukraine, Turkey and Moldova possess strong comparative advantages too. I used Herfindahl-Hirschman-index to determine the market concentration. Based on it – excluding the high Chinese market share between 2006 and 2010 – the world market of concentrated apple juice can be considered as a market with a moderate concentration. Based on the secondary research the study has a detailed examination of cause and effect relationships behind the RCA and HHI index results
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Investigation of Potato (Solanum tuberosum L.) Salt Tolerance and Callus Induction in vitro
51-55Views:225Potato production plays an important role in Hungary and the other countries of Europe. Consumption of potato products has increased to a large extent during the past several years. We can satisfy market demands with high quality and virus-free varieties.
Results of potato production depend on tolerance/resistance to abiotic stresses. In many cases, increased concentration of NaCl causes yield loss. Selection of salt tolerant varieties proved to be a difficult problem. Nowadays, the salt tolerance of potato varieties can be determined by cell/tissue/ protoplast techniques. Somaclonal variation provides a great potential for selection of lines resistant to salt stress. In vitro shoots and callus, derived plantlets selected for salt tolerance/resistance provide material for micropropagation.
In vitro shoot development of potato (Solanum tuberosum L. cv. Kuroda) was investigated under salt stress (40 mM, 80 mM, 120 mM NaCl) conditions. Shoot heights of plantlets cultured under salt conditions were lower than the control through the investigation. However, the shoot development of plantlets originated from in vitro meristems was almost at the same level as the control under 40 mM NaCl concentration.
There was no significant difference in the in vitro biomass production between control and treatment with 40 mM NaCl concentration. We measured a significant decrease in dry-matter mass under 120 mM NaCl concentration. There is a need for more investigation of different genotypes and for a conclusion as to whether in vitro tolerance could occur under in vivo circumstances in plants originated from somaclones as well.
Under in vitro conditions, we investigated shoot and leaf callus initiation using different culture media with different 2,4-D concentrations. Under dark conditions, callus induction of shoot/leaf decreased as the 2,4-D concentrations increased.
In light conditions, there was a little callus induction, while callus initiation from the shoot from 5 μM to 12 μM 2,4-D concentration showed a significant increase -
Measuring of nitrogen leaching using ceramic suction cups at different locations
10-17Views:173Ceramic suction cups were used for the measurement of N-concentration in soil solutions under different soil and climate conditions in both field experiments of Rostock University and Agricultural University of Debrecen (Hungary). Depending on the soil utilisation the change in the N concentration of the soil solution can be proved on both sites.
The experimental field of Rostock University can be characterised by its high groundwater table. The nitrogen concentration of soil solutions in the different soil layers were determined by the trend downward of water. In the dactylis (Dactilis glomerata) experiment, the quadruple treatments involved the following: with and without N-fertiliser, with and without harvesting, respectively. In the lower soil layers, the least rising N concentrations were established in case of the treatment without N-fertiliser combined with harvesting. The nitrogen leaching calculated from the infiltrated water quantity and the nitrate N concentration increased in the following order: without N-fertiliser, with harvesting < without N-fertiliser, without harvesting < with N-fertiliser, with harvesting << with N-fertiliser, without harvesting.
The field experiment site of Debrecen can be characterised by a low groundwater table. The effect of N-fertilisation on the nitrate-N concentration of soil solution in the soil layers can be stated unanimously. Permanent nitrate-N leaching cannot be established due to the water upward movement under semiarid climate conditions. Intermittently transfer of nitrate-N between the soil layers is probable in cases of remarkable precipitation. -
The effect of cobalt-chloride on the production parameters and homogenity of barramundi (Lates calcarifer) larvae
21-25Views:395Barramundi (Lates calcarifer) is a predatory fish species native in Southeast Asia and Australia. Based on the geothermal potentiality of Hungary the high market potential warm-water fish barramundi can be produced economically. Living nourishment organisms such as Artemia nauplii play an essential role in the larval rearing of barrramundi. However, zooplanktons in natural aquatic enviroments contain minerals in a higher concentration than the usually fed newly hatched Artemia. Therefore the goal of recent study was to investigate the effect of cobaltchloride on the larval growth and survival of barramundi when fed individually and combined with Zn and Mn supplemented Artemia.
In our experiment a 24 hours period was used for the enrichment of newly hatched Artemia nauplii with cobalt chloride in itself, as well as in combination with zinc sulphate and manganese chloride. A total of 1900 barramundi larvae from 15–30 day post hatching were fed with supplemented Artemia in 9 groups of treatments in duplicate. The growth performance and elemental concentration of 40 larvae from each group was determined. All the groups produced significantly improved growth compared to the control (p<0.05). The lower concentration of individual Co supplementation resulted in a higher growth performance while the opposite dose relation occured when combined the Co with Mn. Cobalt had a significant negative effect on the Mn uptake of the larvae – significantly higher Mn accumulation compared to the control group was only observed when Mn was fed in itself (p<0.05). In case of Co-Zn-1 and Co-Zn-2 treatments significantly higher Zn concentration was measured than in the others (p<0.05).
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Using crop analysis in the precision nutrient supply system of maize
183-186Views:187The effect of the N, P and K supplies of soil on the grain yield and N, P and K status of maize was studied in a long-term mineral fertilisation experiment between 2001 and 2008 and nutrient supply limit values were determined to plant analysis. Based on the interaction between the N concentrtion of maize leaves measured at the beginnig of tasseling and grain yield, the satisfactory limit value of N supply to reach 10–14 t ha-1 yield was between 2.0–4.0%. Leaf analysis at the beginning of tasselling indicated that better P and K supplies were associated with a higher P and K concentration in the maize leaves. Correlation analysis on the P concentration of the maize leaves and the grain yield showed that at a grain yield level of 10–14 t ha-1 a P concentration of 0.20–0.37% represented a satisfactory P supply level. The satisfactory K supply limit value to reach 10–14
t ha-1 grain yield was 1.5–2.6%. -
Effect of Copper, Zinc and Lead and Their Combinations on the Germination Capacity of Two Cereals
39-42Views:649The majority of researchers have studied the following group of microelements: B, Zn, Mn, Cu, Na, Co, Mo, I, Sn, Cl, Al, V, F, Cr, Hg, Cs, Li, Cd, As, Th, Rb, Cr, W, Ti, Sn, Se, Ba, Br. Sporadically, the following elements have been mentioned too: Au, Ra, Hg and Pb. In this study, the effects of copper treatments and their combination with zinc and lead microelements on the germination of maize and barley were investigated using different concentrations of these microelements. Six treatments were used: 1. Copper-sulphate (CuSO4) applied alone, 2. Zinc-sulphate (ZnSO4) applied alone, 3. Copper applied with zinc, 4. Lead-nitrate (Pb(NO3)2) applied alone, 5. Copper applied with lead and 6. Untreated control. Maize (Kiskun SC 297) and barley caryopsis were treated with copper and zinc solutions in the following concentrations: 0.03%, 0.003% and 0.0003%. Maize and barley caryopsis were treated with these solutions for 12 and 24 hours. Maize and barley caryopsis were also treated with lead solutions Pb(NO3)2 with different concentrations: 0.0005%, 0.005% and 0.05%. Maize and barley were treated with these solutions for 12 and 24 hours. In the combined treatments (3 and 5), the same concentration was used for each microelement as in treatments 1, 2 and 4. Control treatments were treated with water for both plant species. Our results showed that copper microelements significantly inhibit germination compared to the untreated control. The toxicity of copper is higher if concentration increases. Zinc microelements also inhibit germination, however its effect highly depends on the microelement concentration. Treatments of copper + zinc also inhibit germination. The two microelements applied together cause more phytotoxicity than they do alone. Lead is highly toxic to plants even in low concentrations. The toxic effect on germination dramatically increased when lead was applied with copper.
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Heavy Metals in Agricultural Soils
85-89Views:143The soil constitutes the basis of the food chain. To keep soil conditions in a good trim is very important, it’s part of the sustainable development and of producing food supply harmless to health.
In some cases, soil productivity is the only important part, qualitative requirements or economical characteristics can improve it. The soil is threatened by two danger factors: the soil degradation and the soil pollution. The accumulation of different harmful and/or toxic substances in the soil is well known. Heavy metals constitute a part of it. Metals in the soil and in the soil-solution are balanced. This balance depends on the type of the metal, on the pH, on the cation-band capacity of the soil, on the redox relations and the concentration of cations in the soil.
To be able to handle the metal contamination of the soil, it is important to estimate the form, the possible extension and the concentration of metals.
Of course, the different types of soils have different physical-chemical, biological and buffer capacity, they can moderate or reinforce the harmful effects of heavy metals. To draw general conclusion of the dispersion and quantitative relations on the metals originated from different contamination sources is hard, because in some emissive sources contamination is limited in small areas but on a high level, some others usually expand on larger areas, and as a result of equal dispersion, the contamination’s level is lower.
Heavy metals – unlike alkali ions – strongly bond to organic materials, or infiltrate in a kelát form. Their outstanding characteristic is the tendency to create metal-complex forms. Kelats take part in the uptaking and transportation of heavy metals. Heavy metals exert their effects mostly as enzyme-activators.
The metals cannot degrade in an organic way, they accumulate in living organisms, and they can form toxic compounds through biochemical reactions.
Lot of the heavy metals accumulate on the boundaries of the abiotic systems (air/soil, water/sediment), when physical or chemical parameters change, and this influences their remobilization.
Human activity plays a great part in heavy metal mobilization, results in the human origin of most biochemical process of metals.
To understand the toxic influence of accumulated metals of high concentration, their transportation from soils to plants or their damage in human health, must clearly defined and investigated.
For effective protection against soil pollution, the types and levels of harmful pollution to soil must identified, regarding legal, technical and soil-science aspects, preferable in a single way. Difficulties in this area mean that toxicity depends on loading, uptake, soil characteristics and living organisms (species, age, condition etc.), furthermore, local and economic conditions considerably differ. -
Seasonal variations in somereproductive parameters of Dorper Rams in Hungary
17-20Views:611The purpose of the present study was to test the hypothesis that season has an affect on semen quality and scrotal circumference of Dorper rams. The experiment was carried out with six Dorper rams aged between 15 and 18 month. Semen samples were collected with artificial vagina and volume, concentration (x 109/ml), total sperm number/ejaculate (x 109), mass motility (0–5), progressive motility (%), scrotal circumference (cm) was observed. Significant differences (P<0.05) were observed in concentration, total sperm number/ejaculate, scrotal circumference in different seasons. Volume was the highest in autumn (1.4±0.5 ml) and the lowest in the spring (1.3±0.4 ml). Concentration of semen was lower in spring (2.6±1.5 x 109) and summer (3.3±1.5 x 109) as compared to fall (4.1±1.1 x 109) (P<0.05). Regarding total sperm number/ejaculate (x 109), scrotal circumference (cm) all the seasons differed significantly (P<0.05), although the season had no effect on mass motility and progressive individual motility (P<0.05). In conclusion the present study showed that semen quality parameters and scrotal circumference of Dorper rams were better in autumn than in the other sea- sons.
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Analysis of maize and sunflower plants treated by molybdenum in rhizobox experiment
11-14Views:449In this study, maize (Zea mays L. cv. Norma SC) and sunflower (Helianthus annuus L. cv Arena PR) seedlings treated by molybdenum (Mo) that were cultivated in special plant growth boxes, known as rhizoboxes. During our research we tried to examine whether increasing molybdenum (Mo) concentration effects on the dry mass and absorption of some elements (molybdenum, iron, sulphur) of shoots and roots of experimental plants.
In this experiment calcareous chernozem soil was used and Mo was supplemented into the soil as ammonium molybdate [(NH4)6Mo7O24.4H2O] in four different concentrations as follow: 0 (control), 30, 90 and 270 mg kg-1.
In this study we found that molybdenum in small amount (30 mg kg-1) affected positively on growth of maize and sunflower seedlings, however, further increase of Mo content reduced the dry weights of shoots and roots. In case of maize the highest Mo treatment (270 mg kg-1) and in case of sunflower 90 mg kg-1 treatment caused a significant reduction in plant growth.
In addition, we observed that molybdenum levels in seedling were significantly elevated with increasing the concentration of molybdenum treatment in comparison with control but the applied molybdenum treatments did not affect iron and sulphur concentration in all cases significantly.
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The effect of rising concentration of atmospheric carbone dioxide on crop production
81-84Views:217In the atmosphere, the amount of carbon dioxide and other greenhouse gases are rising in gradually increasing pace since the Industrial Revolution. The rising concentration of atmospheric carbon dioxide (CO2) contributes to global warming, and the changes affect to both the precipitation and the evaporation quantity. Moreover, the concentration of carbon dioxide directly affects the productivity and physiology of plants. The effect of temperature changes on plants is still controversial, although studies have been widely conducted. The C4-type plants react better in this respect than the C3-type plants. However, the C3-type plants respond more richer for the increase of atmospheric carbon dioxide and climate change.
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Challenges in the mangalitsa sector – present and future
147-153Views:200The Hungarian mangalitza pig sector has experienced numerous structural changes in the past few decades. The increased demand in the foreign markets for the mangalitza pork, changes in the domestic consumer perceptions have increase the number of sows and breeders. After the European Union’s accession started a significant growth in the sector, which was due to the target programme from the year of 2005 for the keeping of animals representing high genetic value, as the breed is indigenous. From 2008 continuous decline can be observed. The main reason for the decrease was the drastic growth of feed costs because of the drought damage in 2007, and the global economic crisis. Since 2010, a further period of supports has been in place, it extands the willingness to keep mangalitza pigs and the number of sows continuously increasing. On the basis of the average farm size (58 sows/farm) in 2011 it can be stated, that after our EU’s accession the medium-sized farms became stronger. To evaluate the geographic concentration of the mangalitza livestock it was analysed the data of sow number among the years of 2000 and 2011. The geographic concentration of mangalitza stock has a medium value over each year under the survey period, except the years of 2004–2007, when the values of Herfindahl-Hirschman index (HHI) have low degrees. Due to the subvention period of indigenous breeds it was established more mangalitza farms in different parts of Hungary. Despite of the growth since 2000, numerous problems are in the segment. The aim of the study is to find out the sectoral problems, which are completed with the objectives tree and draw up the main activities to solve the problems. After estimating the SWOT-matrix according to the methodology of the strategic analysis and the discussions with the secretary of National Association of Mangalitza Breeders is prepared the sectoral problem- and objectives tree. The core problem of the mangalitza sector is that the origin of mangalitza products are not certified in the domestic markets. The reason of it is that the breeders don’t request the certificates of fattening pigs, so it can appear not real mangalitza product on the markets as mangalitza. To solve the problems it is drawn up different suggestions in the objectives tree. This analysis is an useful tool for the decision makers in the mangalitza sector to evolve strategic plans on behalf of the efficient cooperations among chain actors.
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The significance of sour cherry in iron supplementation
101-104Views:306The iron concentration of ’Csengődi csokros’, ’Debreceni- and ’Érdi bőtermő’ , ‘Éva’, ’Kántorjánosi’ ,’Petri’ and the ’Újfehértói fürtös’ cultivars was determined by ICP-MS. Furthermore the Vitamin C, L-Malis acid and Citric acid concentration of samples were measured.
Our results show that large amount Fe2+ (average 20.5 mg kg-1) accumulates in the pulp of sour cherry. Besides, the concentration of Vitamin C, L-Malis acid, that increase the absorption of iron, are high. Based on these results, the sour cherry and the products of sour cherry play an important role in the forming of iron content in body.
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Determination of the validation parameters of inductively coupled plasma mass spectrometer (iCP-mS): response curve linearity in the case of arsenic and selenium
67-71Views:412In the field of elemental analysis inductively coupled plasma mass spectrometers (ICP-MS) have the best sensitivity that means the lowest limit of detection, subsequently their applicability for the detection of essential and toxic elements in foods and foodstuffs is prominent. For the most elements could be measured the detection limit is between μg kg-1 (ppb) and ng kg-1 (ppt) e.g. for arsenic and selenium.
Considering an analytical task (sample type, analytes and their concentration, pretreatment procedure etc.) the applicability of an analytical method is determined by its performance characteristics. The purpose of validation is to ensure that the method would be used fulfills the requirements of the given task. In this article we describes one of the performance characteristics, the linearity, and the whole validation procedure aims measurement of arsenic and selenium in foodstuffs by inductively coupled plasma mass spectrometer (Thermo XSeries I.); but because of the limited number of pages the results are demonstrated only for arsenic.
The linearity of calibration was evaluated in three concentration ranges (0.1–1 μg l-1; 1–10 μg l-1; 10–50 μg l-1), with nine line-fit possibilities (without weighting, weighting with absolute or relative deviation; with or without forcing the curve through blank or origin) and different methods (graphical examination, correlation coefficient, analysis of variance).
The best method to ensure the linearity of correspondence between signal and concentration was the ANOVA test. In view of calibrations it was found that the range of 10–50 μg l-1 could be regarded as linear with four line-fit possibilities, and was non-linear between 0.1–1 μg l-1 and 1–10 μg l-1.
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Optimization of inductively coupled plasma mass spectrometer parameter’s to measuring arsenic and selenium
59-64Views:629In the last decades an increased interest has been evolved about arsenic and selenium. The aim is to understand the environmental, agricultural and biological role of the these elements. In case of arsenic the mayor reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking waterbases of some Asian countries besides Hungary. The toxicity of higher level selenium content is also known, nevertheless selenium is essential for some biological functions. Considering its esssentiality, in our country the insufficient selenium intake rate couse lack of selenium. Measuring the concentrations of these elements are cruital but not satisfactory information, but the speciation, that is the form of an element presented in a sample is also required.
In both cases the most suitable method to determine concentration is the inductively coupled plasma mass spectrimetry. My objective was to optimase the changeable parameters of the ICP-MS for reaching the lowest (the best) detection limit. For this porpuse I have investigated the effect of parameter change on nett signal intensity and relative signal intensity. With the optimased parameter settings the limit of detection for arsenic and selenium were determined, which are 0,032 ng cm-3 for arsenic, and 0,097 ng cm-3 for
selenium. -
Leaf protein analyses in order to utilise the leaf shoot of artichoke
43-47Views:254The constat growth of the Earth’s population brings with itself a higher demand for food and protein not only in human nutrition but also for the feeding of livestock. Currently, the feed industry is mainly built onseed-based protein, wherethebaseplant is soybean, which is large lycovered by imports in Hungary, similar toother European countries. However, the long-term economically sustainable lifestock breeding demandschanges which has also worked out strategies. An alternative protein sources could be green leafy plants.
In current work the Jerusalem artichokes as an alternative source of protein was studied, compared to alfalfa as a valuable protein plant. Our results indicate that fiber fraction ofJerusalem artichoke shootswas 34 to 37% after pressing in the autumn period while alfalfa slightly lower values were obtained (30%). On the other hand extracted green leaf protein concentration was higher in alfalfa than in Jerusalem artichoke. Along with this higher protein content could be measured from the leaf protein concentration of alfalfa and almost each amino acids were more, as well comparing to Jerusalem artichoke.
Overall, the alfalfa proved to be advantageous as expected both in leaf protein extraction efficiency, both regarding the content of the protein in the Jerusalem artichoke. However, considering aminoacid composition and green biomass production, Jerusalem artichokecould be a promisingplant species asplant protein sourceinthefuture.
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The effect of lead and copper heavy metal salts on soil microorganisms under laboratory circumstances
55-59Views:299he population dynamics of calcareous chernozem soils polluted with different concentrations of lead and copper heavy metal saline solutions was examined.
The experiment was carried out in the soil biological laboratory of the Institute of Agricultiral Chemistry and Soil Science at DE AGTC MÉK in 2012. For the determination of the concentration of the undiluted stock solutions we multiplied the smallest toxic concentration values of the MSZ 08-1721/1-86 Hungarian standard by forty. The intermediary concentrations of the treatments were produced with adequate dilution of the stock solutions until a dilution level equal to the values of the standard. The statistical evaluation of the data was performed with ANOVA (Analysis of Variance) including the determination of the standard deviation and significant difference. Investigating the effects of the different treatments on the soil microbes we established that both heavy metal saline solutions had a negative effect on the population dynamics of bacteria and microscopic fungi living in the soils. The negative effect of copper – as a potential toxic micro nutrient – turned out to be less strong than the negative effect of the toxic lead. According to our results the correction of the treatment levels is recommended in order to further tolerance examinations and the determination of the tolerance levels.
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Parameter optimization of an inductively coupled plasma mass spectrometer for measuring arsenic and selenium
81-85Views:383In the last decades, an increased interest has evolved in arsenic and selenium. The aim is to understand the environmental, agricultural and biological roles of these elements. In the case of arsenic, the major reasons are the relatively high concentration of arsenic in marine biota (mg kg-1) and the arsenic contaminated drinking water bases of some Asian countries, as well as Hungary. The toxicity of higher level selenium content is also known; nevertheless, selenium is essential for several biological functions. Considering its essentialness, in our country, the insufficient selenium intake rate causes a lack of selenium. Measuring the concentrations of these elements provides crucial, but unsatisfactory information, as the speciation, i.e. the form of an element presented in a sample is also required.
In both cases, the most suitable method to determine concentration is inductively coupled plasma mass spectrometry (ICP-MS). Our objective was to optimize the variable parameters of the ICP-MS to attain the lowest (the best) detection limit. For this purpose, we investigated the effect of parameter change on net signal intensity and relative signal intensity. With the optimized parameter settings, the limits of detection for arsenic and selenium were determined, which are 0,032 ng dm-3 for arsenic, and 0,097 ng dm-3 for selenium. -
Sour cherry seed as an industrial gamma tocopherol source
27-33Views:300In our experiments sour cherry kernels were investigated. The kernels of different sour cherry cultivars were originated from Újfehértói Gyümölcstermesztési Kutató és Szaktanácsadó Nonprofit Közhasznú Kft. Tocopherol profile, oil yields and nutritional value were stood in focus of examinations.
We started processes preparing of sour cherry kernels. After the preparation recovered non-polar fraction was investigated. We worked with formerly developed chromathographic system, which is capable to separate different tocopherol isomers efficiently. We measured concentration of each tocopherol isomers and registered those and their relation in different oil samples.
According to received data, alpha-, gamma- and delta-tocopherol appeared in samples, but beta-tocopherol could not be detected. It became clear that level of gamma-tocopherol is far higher than alfa- or gamma-isomer, it is concentration exceeded 1–1.5 mg ml-1.
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Daily soil carbon dioxide flux under different tillage conditions
141-144Views:410Over the last few years, warming of the atmospheric layer near Earth's surface is increasingly experienced and researchers have also established that concentration of numerous greenhouse gases have risen over the past two centuries value. Change is basically a legitimate process - considering atmospheric concentration as well - but the change experienced during the past centuries could not have become this critical without the contribution of human activity. Due to the nature of the greenhouse effect, the result of a very fragile, complex process is experienced currently on Earth, which can be significantly unbalanced even by a slight change. Carbon dioxide emitted from the soil is involved in the global cycle and has an impact on the greenhouse effect. The rise in soil respiration may result in the further intensification of warming. In the scope of the present study, it was examined how carbon dioxide emissions of the soil evolve over a day. The results have been established based on the comparison of the effects of different parts of the day, tillage methods and irrigation.
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Characterisation of a thermotolerant yeast, Kluyveromyces marxianus CBS712
7-13Views:319Fermentation at high temperature with application of thermotolerant microorganisms is a technological advantage in bioethanol production. Among the yeasts, K. marxianus has outstanding thermotolarance. The industrial application of the IMB3 strain occurs usually at 45C. The final aim of our project is the genetic modification of the K. marxianus CBS712 strain in order to achieve ethanol production at higher temperature than the currently applied. This requires the characterization of the CBS712 strain, with special attention to the determination of the temperature limit of its growth and the amount of the ethanol produced. The temperature limit of growth was 48C in YPD medium. Elevation of the temperature above 45C led to an exponential drop of the cell viability. Ethanol production was tested in shaking flasks, in MYFM medium, under oxigene limited conditions, applying variable concentrations of glucose (12–20%) and different temperatures (45–47 ºC). Preliminary results have revealed that the elevation of glucose concentration increased the amount of ethanol produced. The amount of ethanol (appr. 5%)+ produced at the highest glucose concentration was not different at the tested temperatures (45, 46 and 47 ºC). The observation indicates the potential in raising the thermotolerance of the strain.