Search
Search Results
-
Research on rheological characteristics of winter wheat varieties
266-272Views:140The gluten examination test is dominant in Hungary, comparing it to the qualification system of other countries. The determination of alveographic parameters is a basic criterion of winter wheat specifications in some western and southern European countries.
In the Hungarian and foreign winter wheat qualification systems, in the standards, there is no limit for extensographical parameters. Customers dictate the limits and make their claims as to the specifications.
We analyzed the alveographic and extensographical parameters of 19 winter wheat varieties grown by the Cereal Research Non-Profit Company in Hungary, and we made a comparison between the results in the challenges of the European Union expectations.
Examining the information of alveographic and extensographical values, we found that the experimental varieties provide high base to flour types suitable for baking bread and baker’s ware. The GK Élet, GK Petur, GK Memento, GK Csillag, GK Kapos and GK Marcel varieties can meet the market of paste flour needs, too.
In the variety series, the GK Kalász represents the highest values and the GK Garaboly shows the lowest parameters regarding the alveographic W and the resistance to extension.
On the basis of relation, we can establish that we can estimate the following little known and used quality indexes: the extensographical resistance to extension with extensographical energy and the alveographic W value, the extensographical energy with the alveographic W value and the alveographic P and L values with the other alveographic parameters. -
A new model for predicting carbon storage dynamics and emissions related to the waste management of wood products: introduction of the HWP-RIAL model
75-81Views:107The ability of wood products to store carbon allows for their significant contribution to the climate mitigation efforts and the emission reduction commitments set by the EU. In order to optimise the carbon storage capacity of wood products, it is important to take climate mitigation aspects into consideration as much as possible during their production, use and waste management. The aim of this study was to quantify the effects of product development, recycling, and waste management technologies on carbon storage and emissions. In the frame of the ForestLab project, a new model and decision support tool was developed, which is able to predict the duration of carbon storage of wood products and the evolution of emissions from them. The developed HWP-RIAL model (Harvested Wood Product Recycling, Incineration And Landfill model) uses the methodology of the Intergovernmental Panel on Climate Change (IPCC) to calculate emissions, which is also used in the National Greenhouse Gas Inventory report. It combines the IPCC waste model with equations describing the carbon storage and emissions of wood products, and the model is also supplemented with a self-developed recycling and waste routing module. This paper provides insight into the operation of the model by following the life cycle of 200,000 m3 particle board.
-
Comparative Study of Dutch and Hungarian Environmentally-friendly Apple Orchards on Potential Ascospore Dose of Apple Scab
31-36Views:91In a 2-year study, Dutch and Hungarian environmentally-friendly apple orchards were compared as regards the amount of apple scab primary inoculum. The PAD (potential ascospore dose) method was used to quantify the potential amount of primary inoculum (ascospores) per m2 orchard floor. Applying this method, the number of lesions per m2 of leaf in the autumn (LD), the proportion of the orchard floor covered by leaf litter at bud break (LLD) and potential ascospore dose (PAD) were determined. In autumn, LD values ranged between 2.2 and 13.5 in the integrated orchards, while in the organic orchards the values were between 42.5 and 106.2, with especially high values in the Dutch organic orchard. LLD values ranged between 24 and 43% at bud break in both countries. PAD values were 10-60 times higher than those of the integrated orchards. The PAD values were between 673 and 4275 ascospore/m2 orchard floor in the integrated orchards, while in the organic orchards these values were 37102 and 52390 ascospore/m2 orchard floor, respectively. On the basis of the recorded primary inoculum quantity, the Dutch integrated apple orchard is considered to be excellently protected, while the Hungarian orchard has a medium level of protection. However, both countries’ organic orchards can be regarded overall as being very poorly protected. Accordingly, in the integrated orchards the predicted epidemic risk is low or medium, while in the organic orchards it is very high on the basis of PAD values.
-
Acquisition practice prior to commercial use in the European Union
153-158Views:76The target of 3% of GDP spent on R&D by the Lisbon priorities seems to be still far away from the reality in some European countries and at EU level as well. The economic crisis determined the priorities and it makes more complicated to dedicate more budget on Research and Development. New methods, new measures are needed in the European Union. Measures that supporting the demand side of the innovation and generates market-pull affects. The Europe 2020 Innovation Union Flagship Initiative dedicates a special role to precommercial procurement for the upcoming 10 years. Nowadays, there are more and more European countries running pilot projects adapting the American SBIR (Small Business Innovation Research) programme.
-
Nutrient and water utilisation analyses of maize on chernozem soil in a long-term field experiment
77-82Views:182We have conducted our research at the Látókép Research Farm of the University of Debrecen RISF Centre for Agricultural and Applied Economic Sciences during the cropyears of 2007, 2008 and 2009, on chernozem soil. In the case of crop rotation three models were set (mono-,bi- [wheat, maize] and triculture [pea, wheat, maize]). The five nutrient levels applied during the treatments were as follows: control [untreated], N60P45K45, N120P90K90, N180P135K135, N240P180K180. The conclusion of our results was the following: the crop rotation, the nutrient supply and the amount of precipitation all influenced the quantity of maize yield. As an effect of the increasing nutrient doses yield increase was experienced compared with the control treatments. In the average of the years the highest increase in yield excess/1 kg of NPK fertilizer was measured in the case of the monoculture (13 kg ha-1). As a consequence of is soil extorting effect the monoculture responded more intensively to the nutrient supplementation than the biculture or the triculture in the studied cropyears. In addition, we have observed that the three-year average yield amount per 1 mm precipitation was significantly influenced by the nutrient reserve of the soil. In the monoculture during the control treatment this value was 25 kg mm-1, the value measured in the case of the biculture turned out to be more favourable (42 kg mm-1).
-
Effect of genotypes and cropyear on thedifferent cultivation parameters of asparagus (Asparagus officinalis L.)
31-34Views:158The experiments were performed in the Research Institute of Nyíregyháza Research Institutes and Study Farm, Centre for Agricultural Sciences University of Debrecen of 1500 m2 asparagus plantation. The asparagus was planted in 2011. We were determine the number of shoots, plant height and fold thicknes of asparagus hybrid. We can continuously monitored the growing parameters of asparagus. The harvest of asparagus was started in 2013, so we were able to measure to quantity. The weather was characterized by warm and matched precipitation in the growing season (April–July) in 2011 and 2012. Contrarily, the weather was very hot and dry after the late frost in 2013. The Vitalim produced the largest quantity, then the Cumulus and the end of Grolim hybrid. Number of shoot and plant height sequence is identical to the previous one. The Grolim asparagus hibrid has the largest basal diameter.
-
Evaluation of bioactive substances in different parts of the root in beetroot (Beta vulgaris ssp. esculenta var. rubra)
33-38Views:30Beetroot, also known as Beta vulgaris ssp. esculenta var. rubra, is a root vegetable that contains a substantial quantity of bioactive elements, notably antioxidants and anti-inflammatory properties. These bioactive compounds, including betalains, phenolics, and flavonoids, contribute to the health-promoting properties of beetroot. The antioxidant and anti-inflammatory effects of beetroot have been extensively studied in preclinical and clinical settings. The active compounds in beetroot have been reported to provide benefits in reducing the risk of various diseases, our research provides a comprehensive analysis of the bioactive compounds the inner and outer skin parts of the root structure. The findings aim to contribute to a deeper understanding of the potential health benefits associated with specific beetroot root components. Furthermore, the results have implications for optimising beetroot cultivation and processing for enhanced nutritional value. This research not only advances our knowledge of the phytochemical profile of beetroot outer skin but also offers valuable insights into the broader field of plant biochemistry and its applications in promoting human health and nutrition.
-
The Role of Multimedia in General and Economic Vocational Training
81-83Views:52The subject of my study is the methodological question of one of the topics of operation research as an application field of mathematics. The mathematics and operation research team of our institute aims to provide up to date knowledge to our students of both regular and distance education courses. Each topic has been allotted to a different teacher, thus my responsibilities include Networks and their economic application, and my ambition is to achieve increasingly good results teaching this subject. The distance education course of our college provides the opportunity to try a wide range of methodological methods.
-
Examination of the effect of pelleted poultry manure products on a sunflower test plant in a laboratory model experiment
83-88Views:205Sunflower (Helianthus annuus L.) is one of the most important oil plants in Hungary, with a sown area of more than 627.000 ha in 2019. Sunflower cultivation is primarily important for use in the oil industry, but its role in feed and beekeeping cannot be neglected either. Sunflower adapts well to different production areas in terms of soil demand, yet, as important fact, it must be grown on soils with poorer conditions with intensive nutrient replenishment. From the agri-environmental point of view, in addition to the use of pelleted poultry manure products, which are also new to the consumer market, is of paramount importance. Their advantages include a wide range of nutrients as well as a soil structure improving effect.
In our research, we investigated the starter effect of pelleted poultry manure products applied at different doses (200 kg ha-1 and 400 kg ha-1) on sandy soil with humus using a sunflower plant. The aim of our research to investigate the effect of using pelleted fertilizer products as starter fertilizers on sunflower plant germination, and investigate the effect of poultry manure products on soils.
Based on our results, it can be stated that the pigment content (chlorophyll and carotenoid content) was positively influenced by the higher dose treatments (400 kg ha-1), thus increasing the photosynthetically active leaf area. Compared to the control, the carotenoid content in the test plants of the treatments increased, and the differences of this treatment proved to be significant (p<0.05). Our experiments supported the beneficial effect of pelleted poultry manure-based products in the soil-plant system.
-
Advancement of a Common Wheat (Triticum aestivum L.) Selection System
8-11Views:86All the research in Hungary and other countries in Europe focus on improving the quality of crops and increasing the competitiveness of production.
In this respect, we have to advance the conventional technological elements, reduce the application of pesticides and fertilizers, and produce new varieties suitable for environmentally-sound production. In our crop breeding programs, we applied conventional and biotechnological methods (embryo rescue and double haploid methods) in order to get somaclonal and gametoclonal variations.
We produced winter wheat lines (HP-31-95, HP-82-96) by traditional way, which have high baking qualities and high nutrient efficiency. Some diseases can limit the quantity and quality of a wheat crop. We examined several wheat diseases in our winter wheat candidates, and we found that our progenies have resistance to leaf rust.
With respect to a serious problem was the small quantity of applied fertilizers and the other externals, our research focused onto advance a common wheat selection system, with the help of it, we can handle these problems. We have summarized that our selected progenies can compete with the registered varieties with quality and environmental respect. -
The presence of the undertaken social and economic role in the university's strategy-making
93-96Views:122It is vital to examine the strategic plans and leadership insights of the university, the faculties, the departments and the institutions in the research of the „third mission” of the universities. Because of the undertaken social-economic role in the region it is indispensable to know the turbulently changing environment (hazards – opportunities), to map the competencies available in the university (strengths – weaknesses) and to recognize the relations between the external and internal environment. It is an important factor to define the strategic directions of the departments, examining them in direction dimension (pro-active – adaptive) and in role dimension (top-down – bottom-up). The main objective of this research is to recognize the formal and informal strategic visions of the institutes and departments in the Centre for Agricultural and Applied Economic Sciences of the University of Debrecen, and to examine how these visions are related to the university's role in the regional innovation system.
-
Survey of the chance of secondary salinization in the hobby gardens around Karcag
199-205Views:97Irrigation involves the risk of secondary salinization on certain areas. The increase of the level of salty groundwater or the application
of poor quality (salty) irrigation water can cause secondary salinization. Irrigation from drilled wells is very characteristic in the small
hobby gardens located around Karcag during the frequently droughty summers. Mainly vegetables and fruits with high water demand are
grown in these gardens, hence quite a large amount of subsurface waters are used for irrigation. Water samples were taken from 46 drilled
wells located in the hobby gardens around the town of Karcag and analysed in order to survey the risk of secondary salinization. On the
base of the results of the analyses and some indexes calculated from the chemical parameters of the irrigation waters, it can be established
that none of the investigated wells supply water that is suitable for irrigation without improvement. -
Conceptional Model of Regional Agricultural Water Management System
199-209Views:107Our study focuses on the water management improvement of the Hajdúsági-löszhát (loess ridge). The Hajdúsági-löszhát (loess ridge) is an intensive agricultural area. At the same time, the problem of increasing water demand is still not solved, so towards of safety production irrigation should be improved. To realise this should be known not even agricultural water demands but industrial and urban ones as well, thus a complex water management system is required to be worked out.
In the first part of the research, the water demand in the area is mapped, then a conceptional model of the Hajdúsági-löszhát’s (loess ridge’s) water management system is created. After collecting data the water management scenario is summarized in a real time model splitted into five periods.
During the research, the instruments of spatial informatics (GIS) are used to get acquainted with the variation of the hydrological parameters in space and time. To understand and simulate the different decision making processes and to choose the right decision alternative, a decision support system is created with the use of spatial informatics data.
In addition, considering the potentially right decision alternative, irrigation will be started in practice, an effect and after-effect inquiry will be made, and the results will be analysed, evaluated and summarized. Finally, a suggestion to the most adequate irrigation technology will be made. -
Grassland association stock of plants the examination of the regeneration of a construction
185-191Views:35Due to the drastic change in using the nature like grassland association (one-sided overgrazing – one mowing per a year), by the third year of the experiment in every area, where overgrazing stopped, independently on second sowing and nutrient resupply, Borhidi degradation degree decreased. In the areas where overgrazing with large animal density (sheep) continued, degradation degree was 3.4–5.0 by the third year of the experiment, and Hordeum murinum, which causes animal healthy problems, appeared massively.
-
The Effect of Soil Coverings on Cellulose Decomposition Activity of Sandy Soil
15-20Views:71The purpose of our experiments is to discover the effect of different soil cover matter (agroszövet and black polyethylene) on actual (under field circumstances) and potential (under laboratory circumstances) cellulose decomposition activity. In our field research, the Unger-test was used, and in laboratory research, the Petkov-Markova method was applied. In the first year of the experiment (2000) actual cellulose decomposition activity was significantly higher in covered than in the uncovered soil both in spring and autumn. The difference between the two treatments was significant only in spring. In the spring of 2001 black polyethylene showed significantly the lowest, activity, while in autumn the agroszövet (a porous black polyethylene) showed significantly the highest activity. In the autumn of 2001 the soil covered by black polyethylene gave non-significant,and the soil covered by agroszövet gave a significant higher activity value than the control. Averaging the two experimental years (2000-2001), the actual cellulose decomposition activity was significantly higher in covered soils both in spring (with 30-39%) and in autumn (with 34-69%). Moreover, in autumn a significantly higher value was detected under agroszövet than in any other treatment. The difference between the effect of treatments was not significant. In 2000, the potential cellulose decomposition activity was the highest in soil covered by agroszövet in spring, but in autumn higher activity value was detected in every covered soil than in the control. In the spring of 2001, every covered soil showed a lower, but in autumn a higher, potential cellulose decomposition rate than the control. The activity decreased significantly 27 (agroszövet) and 45 (black polyethylene) percent in spring, and increased no- significantly 8 (agroszövet) and 4 (black polyethylene) percent in autumn. During the two experimental years, we observed on average lower potential cellulose decomposition activity (15-60%) in spring and a higher one (14-43%) in autumn. Neither was significant. The dynamic of potential cellulose decomposition activity averaging 2000 and 2001 showed a slight, the actual cellulose decomposition activity an explicit non-significant upward tendency. There was a strong (r=0,189) correlation obtained between the actual and potential cellulose decomposition activity of soil, and a medium-strong (r=0,673) relationship between the soil moisture content and actual cellulose decomposition activity.
-
Evaluation of blue and red corn landraces
239-243Views:91Corn breeding can be successful only on a broad base of genetic material. The expansion of breeding aims includes the research of germplasm materials. In our experiments, we examined 11 blue, purple and red exotic corn varieties on two locations in Hungary. We conducted a complex study to obtain more information. We studied several morphological and phenological features and determined the most important qualitative parameters (protein, fat, ash). We applied the Tassel Area Index (TAI) under Hungarian conditions and compared it with the pollen producing ability of different genotypes. Results show that there are great opportunities in exotic corn varieties. Their quality exceeds that of the yellow ones in many cases. There are significant differences in yield and nutritional parameters. The favorable nutritional composition is not coupled with great productivity. Based on their flowering time and their agronomical features, they can be cultivated under Hungarian conditions as well. They match the new breeding aims, so they can be used as functional food or energy plants. With their high pollen producing ability and TAI, they can be utilized as markers in future pollen research.
-
Seedling morphology of different wheat genotypes at early stages under hydrocultural conditions
249-254Views:213Consuming “sprouted seeds” is one of the most important factors of a healthy diet. An experiment was conducted in the University of Debrecen, Research Centre of Nyíregyháza (Hungary) in 2014 to analyse some morphological traits of four winter wheat genotypes (Triticum aestivum L.) and one spelt (T. spelta) variety. Our results showed that the spelt wheat variety “Franckenkorn” could maintain higher root length throughout the experimental period. On average, both “Perbetei” and “Franckenkorn” varieties could maintain higher root number compared to the other varieties. The extensive breeding line “1401 HK” had the highest shoot length throughout the whole experiment, being significantly higher than the landrace variety “Perbetei” and both of the varieties “KG Bendegúz” and “KG Kunhalom”. It could be concluded that “KG Bendegúz” cultivar and “Perbetei” landrace seem to be the most suitable for aquaculture techniques. In addition, “1401 HK” breeding line can be the most suitable for the production of juice since the minimal required shoot length (12 cm) was achieved within the shortest period of time after sowing (9 days). This breeding line and “Franckenkorn” can also be suitable for production of “wheatgrass”, because it is consumed without roots. Further research is needed to evaluate nutritional values of these genotypes.
-
Examination of Reproductive Performance of Roe Deer (Capreolus capreolus) in Hungary
33-38Views:101The objective of the research partly is to compare the reproduction performance of the populations living in different regions with regard to some special characteristics (age, condition).
When estimating the age through tooth wear and cementum-layer-counting there was a difference of 0.87 years in favour of the first one (r=0,840; p<0,001). I found cementum layers at 42% of the does in the study after examining the MI teeth.
There was lose connection between the weight (eviscerated, with head and legs) and the KFI (r=0,296; p<0,01), and for further analysis, I used only the KFI as the index for condition.
The regional average KFI varied from 0.24-0.37 in fawns, 0.82-1.73 in does, with individual extremes of 0-4.05. Within the examined regions the highest index belonged to the prime-aged does, while the 1-year-olds had a lower rate, and it was the lowest in the does older than 8 years.
The rate of fertility was between 83,3(ns)-100% as we can see from the presence of the CL. All the examined does were fertile, except in one region, while among the female fawns in two regions I only found three with active ovaries. The average number of CL was 1.5-2.13, and this varied by regions; all in all it was the highest in the 2-7-year-old group (1.96) and in the ones over 8 years (2.00!), while it was lower in the does younger than 1 year (1.90). The high fertility of the does over 8 years is remarkable.
I could examine the number of embryos in two regions during the post-implantation period, and beside 100% fertility I found significant differences among the does, which can be associated with the condition. The ratio of CL carriers and the pregnant does was 100% and 73% in the two regions, the average number of CL were 1.92 and 1.72, while the average embryo number were 1.83 and 1.36 per doe. The difference between the CL and the embryo numbers on the two regions were 5% and 21%. The difference (prenatal loss) is in connection with the age (age class) of the doe. It is possible, however, that in some cases oestrus was not followed by gestation. But in roe deer, owing to the commonly known lack of luteolysis-mechanism (Flint et al., 1994), the regression of the CL of the does that did not get pregnant takes place in December and January, so the CL found in January cannot prove a previous pregnancy, which might have been followed by an abortion.
Although it has to be proven, it seems that the number of the CL (potential progeny) can be associated with the age (r=0,418; p<0,01) and the weight (r=0,312; p<0,01) of the doe, while the embryo number (realised progeny) is influenced by the age of the doe and probably by external factors.
It is essential to continue and extend the research to increase the reliability of the results and their correlation. -
Connections between the roma segregation processes and home circumstances in the outskirts of Hajdúböszörmény
57-61Views:96In order to analyze the social status of local gipsies – both inside the community and among themselves – we conducted an empirical research of 300 participants, in which we took the distinctive spatioand sociostructural features of Hajdúböszörmény into consideration. Furthermore, a second research was also carried out in which we questioned 25 prominent persons. Participants were selected in a way that the study may give a realistic picture of each of the segregated areas of the town (focusing on social, educational and realty issues), thus besides questioning professionals dealing with the concern of gipsies on a daily basis, we decided to involve representatives of the local government as well. The reports concerning the realty status of the segregated areas were supplemented with further material provided by the local government.
-
Using integrated remote sensing methods in the Nagyerdő Natura 2000 area
19-24Views:180The more widely use of GIS, remote sensing technology provides appropriate data acquisition and data processing tools to build several national and international biodiversity monitoring system of environmental protection and natur conservation. The ChangeHabitats 2 is a similar international project, which uses airborne hyperspectral and airborne laser scanning (airborne LiDAR) sources beyond traditional data collection methods to build a monitoring system of Natura 2000 habitats. The goal of our research, on one hand, was to separate the most typical species of trees which can be found in the largest coverage in the research plots of Debreceni Nagyerdő Nature Reserve from field and airborne remote sensing data, use image classification that based on spectral and geometry (height) characteristics of the trees. On the other hand our goal was to evaluate the efficient use of the integration of mobilGIS, airborne hyperspectral and airborne LiDAR data collecting methods to complement or substitut of the traditional, field data collecting methods. We used ArcGIS 10.2 and Exelis 5.0 GIS software for data evaluation, in which the mosaicing, the selection of plots and the spectral image processing were carried out.
-
In memory of József Vörös mycologist (1929–1991) on the 25th anniversary of his death
33-37Views:147Twenty five years ago died professor József Vörös, internationally recognized mycologist, phytopathologist. On the field of microscopic fungi research, especially on taxonomy of plant pathological conidial fungi (Deuteromycetes, Coelomycetes, Hyphomycetes) gained imperishable reputation during his more than 40 years research activity. As author of 17 fungus taxa and publisher of numerous mycological data J. Vörös took place in the history of mycology.
-
Awareness and adoption of a nurse sow management system among small-scale pig farmers in Nakuru County, Kenya
113-120Views:83This study aimed to assess the level of awareness and adoption of nurse sow management strategies among small-scale pig farmers in Nakuru County, Kenya. The research included a survey of pig farmers who visited the Nakuru Agricultural Show in July 2023. A total of 139 farmers were interviewed within 5 days of the show. The corresponding author interviewed the respondents in a face-to-face engagement, where questions on nurse sow management were initially drafted in English and translated into Kiswahili, i.e. the second language in Kenya. The obtained results indicated that farmers came from two main regions of the county: the northern part (54.7%, 76/139) and the southern part (45.3%, 63/139). Levels of awareness and adoption were insignificant between the two groups (χ2, p<0.05). A major obstacle to the adoption of this strategy was identified as feed challenge at 77.7%; (108/139) and market issue at 59.7%; (83/139). This study identified important aspects and limitations that should be considered when developing sustainable productivity development strategies for Kenyan pig farmers. To assist the sustainable growth of small-scale pig production, the authors suggest government measures that shield farmers against exploitation of feed and marketing components, as well as advocating for effective breeding to increase live born.
-
Potential use of bamboo in the phytoremediation in of heavy metals: A review
91-97Views:287There are many literature sources focusing on the phytoremediation of woody plants, but there are only few dealing with the phytoremediation of bamboo plants. Phytoremediation technology has the advantages of little disturbance to the environment and low remediation cost. Bamboo mainly exists in tropical and subtropical regions. As an energy plant, bamboo has a fast growth cycle, large biomass, simple cultivation, high economic efficiency, and convenient harvesting, which highlights the advantages of bamboo in phytoremediation. In addition, bamboo plants have good tolerance and uptake ability to heavy metals and have high application potential and development value in uptaking heavy metal contaminated soil. However, due to climate, temperature and other reasons, bamboo cannot be widely planted in most countries. Research status of remediation of heavy metal contaminated soil by bamboo plants is summarized. The feasibility of its application in heavy metal contaminated soil is discussed in this paper. Aiming at the shortcomings of existing research, bamboo plants have a prospect in the field of plant phytoremediation for the future.
-
Phytopathological aspects of precision agriculture
135-139Views:92This paper illustrates the efforts based on the results obtained in the funding of precision agriculture, during more than two decades of cooperation between University of Debrecen and University of Oradea, within the framework of joint, EU co-financed projects, and put into practice on both sides of the border. Common plant-health databases, interactive Web pages, consultation activities, professional publications, professional training activities, laboratory infrastructure improvements, common research themes proves the progress made to date and create conditions for further development of joint research activities.
-
Selenium speciation analysis of selenium-enriched food sprouts
23-28Views:194In this present study, we prepared selenium-enriched pea and wheat sprouts. During our research we aimed not only to measure the total selenium content of the sprouts but to identify different selenium species.
Scientifical researches show why the analytical examination of different selenium (Se) species is necessary: consumption of all kind of Se-species is useful for a person who suffers in selenium deficit, while there is significant difference between effects of different Se-species on person, in whose body the Se-level is just satisfactory. Biological availability, capitalization, accumulation, toxicity of Se-species are different, but the main difference was manifested in the anti-cancer effect of selenium.
During our research selenium was used in form of sodium selenite and sodium selenate, the concentration of the solutions used for germination was 10 mg dm-3. Control treatment meant germination in distilled water. Total selenium content of sprout samples was measured after microwave digestion by inductively coupled plasma mass spectrometry (ICP-MS). Different extraction solvents were applied during sample preparation in order to separate different Se-species (0.1 M and 0.2 M HCl or 10 mM citric acid buffer). We wanted the following question to be answered: Which extraction solvent resulted the best extraction efficiency? Selenium speciation analysis of sprout sample extracts was performed by high performance liquid chromatography with anion exchange column, detection of selenium species was performed by ICP-MS.
Evaluating our experimental results we have been found that significant amount of selenium of inorganic forms used during germination transformed into organic selenium compounds. There was difference between the amount of Se-species in pea and wheat sprouts and selenium uptake and repartition of selenium species were depended on Se-form used during germination. In addition the chromatogram analysis made us clear as well, that the citric acid solvent proved to be the most effective extraction solvent during sample preparation int he view of organic Se species.