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Phenometric studies on stalk juice and sugar contents of silo sorghum types
41-49Views:70Bioenergies (among them e.g. bioalcohol) can be solutions for the replacement of fossil fuels. For its production, plants with high sugar or starch content can be used. Juice pressed from the stalk of sugar sorghum has high sugar content (14-17%) that makes it suitable for bioethanol production. During our experiment, we examined 53 restorer male lines; among them 22 were silo type sugar sorghum. We studied the following traits: plant height, breeding time, level of foliation, stalk diameter, characteristics of stalk medulla, juice content of stalk, sugar content of stalk juice. According to examined characteristics, we selected six restorer male lines for studies in the forthcoming years: RL 1, RL 2, RL 3, RL 4, RL 5, RL 9, RL 12, RL 15, RL 18. Their stalk medullas were wet, stalk diameters were medium-thick, sugar contents of juices varied between 17 and 24% at the end of milk mature. Harvest was made in September, they can be classified into early maturation group. Male sterile female lines were the following: SL 1, SL 2, SL 3, SL 4, SL 5. The maintainer male lines were: CL 1, CL 2, CL 3, CL 4, CL 5. In Hungary, there are only a few male sterile female
lines, so we will use these lines for hybrid production during the next years. -
Association analysis of TNNI1/XbaI polimorphism on carcass quality in hybrid pigs
59-62Views:148The contractile protein, which is encoded by troponin I 1 (TNNI1) gene, is located on the thin filaments of slow fibres in striated muscle. TNNI1 protein is a part of the troponin complex which plays an important role in regulation of muscle contraction by preventing actin-myosin interaction in absence of calcium. According to biological role, this gene can be potential marker for meat production related traits. The aim of this study is to define whether the previously reported gene polymorphism (EU743939:g.5174T>C) is connected with the slaughter traits measured in a standard slaughterhouse of the examined four-line European hybrid. The study included data from 404 gilts and barrows from 2 different samples. The polymorphism was detected using PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method with XbaI restriction enzyme. In this study the allele frequencies were found as follows: C: 0.84 and 0.808; T: 0.16 and 0.192. Based on result of the present study no significant impact of polymorphisms on production parameters was found.
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Potential use of bamboo in the phytoremediation in of heavy metals: A review
91-97Views:264There are many literature sources focusing on the phytoremediation of woody plants, but there are only few dealing with the phytoremediation of bamboo plants. Phytoremediation technology has the advantages of little disturbance to the environment and low remediation cost. Bamboo mainly exists in tropical and subtropical regions. As an energy plant, bamboo has a fast growth cycle, large biomass, simple cultivation, high economic efficiency, and convenient harvesting, which highlights the advantages of bamboo in phytoremediation. In addition, bamboo plants have good tolerance and uptake ability to heavy metals and have high application potential and development value in uptaking heavy metal contaminated soil. However, due to climate, temperature and other reasons, bamboo cannot be widely planted in most countries. Research status of remediation of heavy metal contaminated soil by bamboo plants is summarized. The feasibility of its application in heavy metal contaminated soil is discussed in this paper. Aiming at the shortcomings of existing research, bamboo plants have a prospect in the field of plant phytoremediation for the future.
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Genetic progress in winter wheat quality and quantity parameters
71-75Views:137Wheat production is significant branch of Hungarian crop production (with about 1 million hectares of sowing area). Weather anomalies resulted by climate change have increased the importance of biological basis in wheat production. Yield quality and quantity parameters of three wheat genotypes sown on chernozem soil type after maize pre-crop were studied in a long-term field experiment. Yield amount of the studied genotypes varied between 2894 and 8074 kg ha-1 in 2017 and between 5795 and 9547 kg ha-1 in 2018 depending on the applied treatments. Based on our results it can be stated that in both studied crop years the highest yield increment was realized by the application of the nutrient supply level of N30+PK. As the result of the application of the optimum mineral fertilizer level – in contrast to the control – resulted in significant yield increment in both crop years. The results of the long-term field experiment prove that water utilization of the studied wheat varieties / hybrids was improved by the application of the optimal nutrient supply. Furthermore, the water utilization of the latest genotypes was more favorable by both the control and the optimum nutrient supply level treatments. Analyzing the quality parameters of winter wheat using the NIR method it has been stated that the quality results of the well-known genotype (GK Öthalom) were better than those of the new genotypes. A negative correlation between winter wheat quality and quantity parameters has also been confirmed. As the result of the mineral fertilizer application protein and gluten content of winter wheat increased to a significant extent.
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Studies on yield stability in autumn wheat species
61-66Views:78The environmental adaptability of crop production is basically determined by the selection of biological background (plant species and varieties) suitable for the region and the site. The sowing structure adapted to the ecological background increases the yield and decreases the yield fluctuation caused natural effects. Exact long-term trials are essential to develop variety structure of winter wheat production suitable for the given ecological conditions. We have examined the productivity and yield stability of genetically different state registered winter wheat varieties. We have compared the varieties’ yield results in plot trials, at similar agrotechnical conditions, in different cropyears. We have examined the absolute and relative (compared to the mean of varieties) yield of winter wheat varieties. We have valued the yield stability of genotypes with the help of analysis of variance and linear regression equations. We have defined the connection between productivity and yield stability of varieties. We have pointed out the varieties with good productivity and yield stability in given agroecological conditions.
According to the results of our examinations the developing of variety structure suitable for the agroecological conditions could increase the potential and effective yield level of wheat production. -
The Role of the 4M-ECO Agrieconomical Modell in the Crop Cultivation
170-173Views:87Today, c for agricultural use are of ever increasing significance. These provide an opportunity for more accurate planning, and favourably influence the efficiency and economic performance of given enterprise. The relevant literature divides models according to various criteria. The most common is the division between optimising and non-optimising models. Non-optimising models generally endeavour to make the best use of technological lines, machine capacity, while optimising models are used to optimise revenue returns from sales or, occasionally, production costs. In our case, revenue and returns from sales were optimised. The models examined consist of several modules. Which include the following: plant cultivation modules, evaluations (assessment of situation, conception plan, complex corporate evaluation), supplementary sheets (sheets and charts for ancillary plant production, general costs of operation, summary and crops structure optimisation). With the help of the model, annual a particular can be made for an optimal crop structure the resources of the enterprise. This it becomes possible to define the largest net revenue on a corporate level.
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Physiological examination of some industrial wastes under laboratory conditions
241-246Views:221I would like to draw the attention to the different side-products and wastes that contain lots of organic matter, micro and macro elements, and the fact that they do not have any harmful effect. These materials can be used as micronutrient fertilizers, therefore quantity of the produced CO2 and other greenhouse gases will decrease. Compost, sewage sludge and lime sludge were used in our experiments. The usability of these materials in crop production was examined in crop production within laboratory conditions.
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Characterisation of a thermotolerant yeast, Kluyveromyces marxianus CBS712
7-13Views:97Fermentation at high temperature with application of thermotolerant microorganisms is a technological advantage in bioethanol production. Among the yeasts, K. marxianus has outstanding thermotolarance. The industrial application of the IMB3 strain occurs usually at 45C. The final aim of our project is the genetic modification of the K. marxianus CBS712 strain in order to achieve ethanol production at higher temperature than the currently applied. This requires the characterization of the CBS712 strain, with special attention to the determination of the temperature limit of its growth and the amount of the ethanol produced. The temperature limit of growth was 48C in YPD medium. Elevation of the temperature above 45C led to an exponential drop of the cell viability. Ethanol production was tested in shaking flasks, in MYFM medium, under oxigene limited conditions, applying variable concentrations of glucose (12–20%) and different temperatures (45–47 ºC). Preliminary results have revealed that the elevation of glucose concentration increased the amount of ethanol produced. The amount of ethanol (appr. 5%)+ produced at the highest glucose concentration was not different at the tested temperatures (45, 46 and 47 ºC). The observation indicates the potential in raising the thermotolerance of the strain.
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Does the feeding frequency influence the growth performance of European perch juveniles (Perca fluviatilis) during intensive rearing?
123-128Views:120The European perch (Perca fluviatilis) is a predatory fish species. Its aquaculture production is increasing worldwide. Feeding and the frequency of feeding are important elements of intensive fish rearing. The aim of our experiment was to examine the optimal distribution of the amount of feed, at the same feed rations. The experiment lasted 42 days. Three treatments were applied in 4–4 replications. The first treatment was feeding twice per day (T2), the second treatment was feeding three times a day (T3), and the third group was fed four times a day (T4). 10 European perch juveniles were stocked per tank, with an individual mean body weight of 3.93 ± 0.06 g at the start of the experiment. The survival rate (S%) was above 90% for all treatments. The T2 treatments produced the most favourable harvest weight (13.96 ± 0.14 g) and specific growth rate (SGR = 3.08 ± 0.01% day-1), but no significant differences were observed between groups. In terms of feed conversion ratio, the best result was obtained by (T3) (FCR =1.06 ± 0.18 g g-1), but no significant difference was found for this indicator neither. The results of the trial indicate that the feeding frequency does not influence the production parameters.
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The Role and Importance of Variety in Intensive Cherry Production
81-84Views:63Increasing the intensity of plantations is a basic precondition for the renewal of fruit growing in Hungary.
The intensification of production of stone-fruit species is difficult for several reasons. In particular, knowledge of dwarfing rootstock is very limited. In this study, we tried to achieve smaller-sized, intensive crown formation by co-ordinating the date and degree of pruning.
In intensive cherry production, the most important variety-dependent characters determining the formation of the required productive surface are vigour of growth, branching potential and quantity and quality of the buds.
In this paper we have demonstrated that significant differences exist between varieties in these characters. -
Results of Brown Bullhead Fingerling Rearing in Recirculating Fish Production Systems
46-50Views:90Predator fish species are in great demand, both in fish production and consumption. Because of their rarity and perfect meat quality, these species have considerable economical significance and increasing domestic and foreign demand.
The brown bullhead (Ictalurus nebulosus) has been in Hungary for more than 100 years, but not popular because of its early maturation rate, aggressive reproduction and slow growth. Despite of these properties, larger specimens (about 150-200 g) are marketable overseas.
Our objective is to ensure the adequacy of the brown bullhead in intensive fish farming and its biological, technological and economic references. The pre-experiments were performed at the Bocskai Fishing Co-operative in Hajdúszoboszló, where we reared the juvenile age class. In our university’s recirculating system we examinated the brown bullhead from the fingerling age class.
The black bullhead prefers artificial feed, and produces excellent growth parameters and a specific feed-live weight conversation ratio. -
The role of agriculture in regional development
25-30Views:173Agriculture is the leading national economic sector of Hajdú-Bihar county. The proportion of employees in this sector and the share of pro ducts produced in this region is above the country average. The contribution of agriculture to the GDP is 6.3%, as opposed to the average value of the country (3.3%). Based on their primary activity, 8% of the enterprises in the county are classified as agricultural and forestry companies, while this proportion is only 5.3% in the whole country.
Nearly three quarters (71.1%) of the sowing area is occupied by maize and other cereals, mainly wheat. This proportion is slightly more than the country average (69.2%). The proportional area of oil crops - including sunflower – and roughage is nearly the same as the country average. However, there is a significant difference in vegetable production. Due to the higher intensity of vegetable production, the area occupied by vegetables is three times the country average. 19.4% of the crop products, 23.5% of animal products and living animals, 25.8% of the income generated by production factors and 29.8% of net enterprise income of Hungary originates from the North Great Plain region.
The facilitation of creating local markets, e.g. local public food supply, satisfying the needs of food trade with local goods, the necessary awareness raising actions, secondary and higher education of experts, as well as the more specific and targeted utilisation of development resources are agricultural development tasks.
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Heat stress of cattle from embryonic phase until culling
11-22Views:246Heat stress becomes a serious problem in the livestock sector as it affects cows' performance negatively. The objective of this paper review is to investigate the effects of heat stress during the different phases of the life cycle of cows; embryos, calves, heifers, and cows. Heat stress during early maternal gestation affects the ability of embryos to develop increasing the risk of abortion and early embryonic death. Heat stress during late maternal gestation affects the performance of calves and heifers later in their life, as it reduces growth performance, conducts physiological changes, impaired immunity, changes the behavior, and reduces the length and intensity of the estrus in heifers with decreasing in milk production in the first lactation. On the level of cows, milk quality and production, meat quality, and the final body weight decrease under hot temperatures. Heat stress decreases the conception rate, alters follicle growth, and estrous symptoms. Hormones secretion and physiological changes because of the heat stress conduct to impair the immunity system, and in oxidative stress and death in some cases. Same as for calves and heifers a change in the behavior of cows was detected in order to decrease their temperature.
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Determining elements of variety-specific maize production technology
157-161Views:62Our aim was to work out such new maize fertilizer methods and models which can reduce the harmful effects of fertilization, can
maintain the soil fertility and can moderate the yield fluctuation (nowadays 50-60 %).
The soil of our experimental projects was meadow soil. The soil could be characterized by high clay content and pour phosphorus and
medium potassium contents. In the last decade, out of ten years six years were dry and hot in our region. So the importance of crop-rotation
is increasing and we have to strive for using the appropriate crop rotation.
The yields of maize in monoculture crop rotation decreased by 1-3 t ha-1 in each dry year during the experiment (1983, 1990, 1992,
1993, 1994, 1995, 1998, 2000, 2003, and 2007). The most favourable forecrop of maize was wheat, medium was the biculture crop rotation
and the worst crop rotation was the monoculture.
There is a strong correlation between the sowing time and the yield of maize hybrids, but this interactive effect can be modified by the
amount and distribution of precipitation in the vegetation period. At the early sowing time, the grain moistures were 5-12 % lower compared
to the late sowing time and 4-5 % lower compared to the optimum sowing treatment.
There are great differences among the plant density of different maize hybrids. There are hybrids sensitive to higher plant density and
there are hybrids with wide and narrow optimum plant densities.
The agro-ecological optimum fertilizer dosage of hybrids with a longer season (FAO 400-500) was N 30-40 kg ha-1 higher in favourable
years as compared to early hybrids.
We can summarize our results by saying that we have to use hybrid-specific technologies in maize production. In the future, we have to
increase the level of inputs and have to apply the best appropriate hybrids and with respect to the agroecologial conditions, we can better
utilize the genetic yield potential. -
Effect of supplemental pig milk replacer on piglet weight gain and sow backfat thickness
43-47Views:186Sow milk production is the major factor limiting pig growth to weaning. Although the milk production of the sows incremental many environmental factors affect the actual performances. The supplemental milk replacer can be an appropriate solution to ward off disparities and try to equalize the available milk quantity to the piglets according to their appetite, to enlarge the weight of pigs at weaning, ultimately. The aim of the present study was to investigate the effects of liquid milk supplement on weight development and litter performance of the piglets during the suckling period. Data were collected from 60 farrowings, the weight of 649 piglets in a total were measured at birth, 14 days old and at weaning (28 days), respectively. In the control group (n=319) the piglets were suckled and got prestarter feed from day 10. In the experimental group (n= 330) the piglets had got liquid milk replacer in 10.71% solution, from day 10 after birth together with suckling and prestarter feed, as well. Based on the Duncan's multiple range tests, there were no significant differences in birth weight between the control and experimental group but we found significant differences between the 14 days weight and the weaning weight, subsequently. Based on the CV% of weight the experimental group became more homogenous, in contrary to the weight of the control group at the end of suckling period. The milk supplementation can be an appropriate solution to ward off disparities and to equalize the available milk quantity to the piglets according to their appetite, to enlarge and uniform the weight of pigs at weaning, ultimately.
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Effect of sowing technology on the yield and harvest grain moisture content of maize (Zea mays L.) hybrids with different genotypes
17-22Views:118From the aspect of the efficiency of maize production harvest grain moisture content shall be considered beside the amount of harvested grain yield. Hybrids with different genotypes and vegetation period length lose their moisture content different that is affected by row spacing and plant density – among agrotechnical production factors – depending on the given crop year. In the present research work three crop years with different weather conditions were studied (2013, 2014, and 2015). The small-plot field experiment was set up at the Látókép Field Research Centre of the University of Debrecen, Centre for Agricultural Sciences with four replications on a chernozem soil type. The effect of three factors was analysed in the experiment on yield amount and its moisture content. Factors were row spacing (45 and 76 cm), plant density (50, 70 and 90 thousand plants ha-1), while hybrids were of very early (Sarolta: FAO 290), early (DKC 4014: FAO 320, P 9175: FAO 330, P 9494: FAO 390) and medium (SY Afinity: FAO 470) ripening.
In the crop year of 2013 the highest yield was produced – regarding the average of the hybrids – by the application of a row spacing of 45 cm (4.5%, 673 kg ha-1), however there was no significant difference between the yield of the populations of different row spacings. Significant difference (14.9%, 1751 kg ha-1; 6.3%, 583 kg ha-1) could be found in case of yield between different row spacing applications in 2014 and 2015. The effect of insufficiently distributed low amount of precipitation and lasting heat days in 2015 could be revealed in yield amounts and harvest grain yield moisture content results that were lower than in the previous years. In 2015 grain yield moisture content varied between 10.3 and 13.9% in case of a row spacing of 45 cm, while by 76 cm between 11.0 and 13.9%.
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Current Conditions and Opportunities of Biofarming in Hungary
150-156Views:85The aim of organic farming is not to maximize income, but to achieve optimal product quality. It is completed by the tightest possible material, and energy flow within the farm. Organic agriculture significantly reduces external inputs by avoiding the use of chemo-synthetic fertilizers, pesticides and pharmaceuticals. Instead it works with nature to increase both agricultural yields and disease resistance. Total independence of external resources can not be achieved in Hungary due to the small-scale of organic animal husbandry. Some materials in limited quantities can be purchased from external resources, though the group of these materials is strictly regulated. Organic farming harmonizes with the concept of European multifunctional agriculture, because besides farming, it includes social considerations, as it helps to maintain natural resources and the relationship between people and their environment, and provides a living for those living in the region.
As regards organic farming the fertility of the soil and the health of vegetation can be influenced in various ways. Farmers have to be highly skilled and able to manage a farm with great expertise. Generally it can be stated that as the use of non-organically produced products is limited, the opportunities to correct failures made by the farmer are minimal, contrary to conventional farming. Farmers must be intent on developing the tightest material- and energy flow. This means that organic farms ideally have both animal husbandry and crop production. This energy and skill demanding system of farming is compensated by state subsidies, growing market share and relatively high prices for organic products. -
Spatial environment analisys of the bioenergy production and utilization
235-240Views:93The backwardness of the rural areas compared to the cities poses a problem all over Europe. Rural development and the reduction of differences between the development levels of the regions have expressed roles among the programs of the European Union. Member States are even entitled to subsidisation, they just need to manage subsidies economically. In Hungary, a relatively small amount of the population lives in the capital, more than 80% of Hungarians live in rural cities or villages. The opposition between the countryside and the cities is rather intensified and the symbiotic correlation would need to be restored. Many people migrate from the countryside, especially youngsters, as they have no opportunities to find any job. This phenomenon poses big risks because getting a job is usually difficult everywhere and because fitting into a new environment always involves a lot of difficulties. Also from the aspect of the national economy, migration from the rural areas to the cities is a problem. The state budget will face significant excess costs if someone moves from a village to the city. It could cause unpredictable consequences if people leave the villages, as the maintenance and development of the village living space will face a hopeless situation.
Non-renewable energies are restricted and they will not be accessible after reaching a certain limit. People’s everyday activities and the functioning of the economy presupposes the availabilty of the necessary amount of energy. In the future, solution that provide the longterm stability of energy for the world will become increasingly necessary. There is a huge potential in bioenergy, more specifically in biomass. The building of biomass plants and putting them into operation creates jobs in the rural spatial environments. A locally available resource that can help in creating the energy safety of the country and the reduction of the dependence on import. The production of energy crops or the crops whose purpose of use is energy could help in strengthening the multifunctional character of agriculture and it can represent a source of income for those living off of agriculture under the current uncertain conditions. -
Change of cultivation parameters of asparagus (Asparagus officinalis L.) on different nutrient systems
67-71Views:126The experiments were conducted at the Research Institute of Nyíregyháza, Research Institutes and Study Farm, Centre for Agricultural Sciences, University of Debrecen. We have studied the shoot number, plant height and fold thickness os asparagus for 3 years. We have set a fertilization experiment on 1500 m2 of plantations in 2013. We applied fertilizer, manure and sheet manure compost. The set yield we achieved by using fertilizer treatment. The Vitalim produced the largest crop, then the Cumulus and the lowest yield Grolim. We was produced by highest green mass was observed in Vitalim and Cumulus hybrids in each year. The Grolim hybrid produced the smallest shoot and plant height but the fold thickness was longer in the other two hybrids. Overall, the Vitalim hybrid has the best production parameters and each season.
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From Organic to Precision Farming (Contemporary Publication)
81-86Views:66The paper presents a short review of the different types of farming systems:
Biofarming, Organic farming, Alternatíve farming, Biodynamic farming, Low input sustainable agriculture (LISA)
Mid-tech farming, Sustainable agriculture, Soil conservation farming, No till farming, Environmentally sound, Environmentally friendly, Diversity farming
Crop production system, Integrated pest management (IPM), Integrated farming, High-tech farming
Site specific production (SSP), Site specific technology (SST), Spatial variable technology, Satellite farming.
Precision farming
It concludes that the various systems are applicable in different ratios and combinations depending on the natural and economic conditions.
The author predicts an increase in precision technologies , the first step being the construction of yield maps compared with soil maps and their agronomic analysis. Based on this information, it will be necessary to elaborate the variable technology within the field, especially for plant density, fertilization and weed control.
The changes in weed flora during the past fifty years based on 10.000 samples within the same fields using the weed cover method are presented. -
Economic Aspects of Bioethanol Production
30-38Views:67Sustainability and multifunctionality look to be crucial points of the future of developed agriculture. Energy utilization of a part of the available biomass perfectly fits in these expectations. Bioethanol production allows for the substitution of the most expensive and most pollutable energy source, gasoline, by agricultural materials. This article contains a complex evaluation of economic characteristics of this method and calculations for the expectable economic effects of a would-be Hungarian bioethanol program. This essay includes the most important technological knowledge, a comparison between bioethanol and the competitive energy sources (gasoline, biodiesel, MTBE) and the most interesting elements of bioethanol programs operating in foreign countries. Introduced are which participants in the bioethanol chain have financial interests and counter-interests under present economic conditions in the spread of bioethanol by the enumerazation of macro- and micro-economic factors. The statements and consequences are based on my own calculatiosn so I am truly interested in any professional opinion.
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Challenges and agroecological approaches in crop production
75-89Views:165Never has the need been greater for an ecosystem approach to agriculture. As our global population exceeds 9 billion in the next 30 years, with a concomitant demand for agricultural products, ever more pressure will be placed on our agricultural systems. Meanwhile, climate change is altering the ecological settings in which agriculture is practiced, demanding adaptation. Knowledge generated by long-term research will help to address one of the grand challenges of our time: how to meet sustainably the growing world demand for agricultural products – in a way that minimizes environmental harm and enhances the delivery of a diverse array of ecosystem services.
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Effects of Tillage Systems on Physical Status and Organic Matter Turnover of the Soil
42-45Views:154The cultivation technology for those plant, that play a key role in arable land production need to be renewed in order to reduce production costs and to protect arable land. The modernisation of technologies can only be achieved by applying appropriate tillage systems. Our measurements were carried out on chernozem soil with lime deposits at the Látókép Experimental Station of the Center for Agricultural Sciences, Debrecen University, in long term tillage experiments set up in 1989. We examined the typical physical parameters, the albedo, field capacity, the bulk density of the soil, organic carbon content (humus %) and the measured pH-values in the water solution within the two tillage variations. We have also modelled nitrogen cycle formation in different treatments.
A significant difference occured between the albedos of the two soils, which may be the result of significant amounts of stem remaining on the surface in the case of the reduced tillage method. The yellow, waxy stem of maize reflects 21% of the sun’s rays, especially at the beginning of the vegetation period, when its decomposition has only just started. This delys the warming up in early spring, which delays the sowing time of maize and reduces evaporation. In the two tillage variations, the water management characteristics do not differ practically, the wilting point field capacity are in accordance. In reduced tillage methods, the so-called „plough-pan” can be well measured at 15-20 cm, while in winter ploughing it is at 30 cm. The humus % of the soil does not differ in the two tillage variations, but due to the difference in bulk density this means a different humus and organic nitrogen content. The organic nitrogen content is greater in the reduced tillage method. On the basis of pH value evaluations, we could not detect significant differences in the two tillage variations. The organic nitrogen content of areas where reduced tillage method was applied is higher than in areas where conventional winter ploughing was applied. -
The use of essential trace elements in the juvenile rearing of barramundi (Lates calcarifer
33-38Views:158Barramundi (Lates calcarifer L.) is a predatory fish species native in Southeast Asia and Australia. Based on the geothermal potentiality of Hungary the warm-water fish can be reared successfully. Zooplankton in the wild contains minerals in a higher concentration than the usually fed newly hatched Artemia nauplii, therefore essential trace elements, such as cobalt, zinc and manganese play an important role in the larviculture of barramundi. Cobalt is vital in trace amount for many living functions of vertebrates, however, lower number of papers are available considering the nutritional aspects. Nevertheless. improved growth performance was observed in cases of some fish species when diet was supplemented with CoCl2. Zinc and manganese are also vital for optimal growth and accordingly are investigated and applied diet supplements in aquaculture.
The main aim of the recent study is to investigate the effects of cobalt, manganese and zinc on the growth performance and homogeneity of fish when a commercially available dry diet is supplemented with trace elements individually and in combined treatments. A total of 6 treatments were set in a randomized blocked design where the concentrations of the applied elements were 50 mg kg-1 for CoCl2, for ZnSO4 and for MnCl2 individually, as well as for CoCl2 along with ZnSO4 and for CoCl2 along with MnCl2 in combination. Although the production parameters of larval barramundi were positively affected by the addition of trace elements when the retention of minerals occurred through nourishment living organisms, statistically no differences were found between the treatments considering the growth performance of barramundi juveniles either when dry feed was supplemented with cobalt, manganese or zinc (p>0.05). While the use of cobalt and manganese in combined treatments produced a less uniform larvae in size and as a consequence of increased heterogeneity, survival was significantly reduced by the cannibalism, the sizes distribution of barramundi juveniles wasn’t affected by the dry diet supplementation of these elements.
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Analysis of longevity in Holstein Friesian cattle using proteomic approaches
21-25Views:124The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,
we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group.