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The effect of sowing date and plant density of winter oilseed rape (Brassica napus var. napus f. biennis L.) population
213-215Views:175The experiment has been set up in the University of Debrecen Látókép Experimental Station in three different years (2014, 2015 and 2016), three different plant densities 200, 350 and 500 thousand ha-1, four replications of the same nutrient supply with using a line spacing of 45 cm. In the experiment, the fore crop was winter wheat in each year. The amount of weeds was observed five times in the last experimental year (2016/2017). In the three experimental years, the highest yield was harvested from the early sowing plot with the highest plant density. On the basis of the Pearson’s correlation analysis there was significant negative correlation (r=-0.583) between the effect of the annual year and yield of the hybrid.
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The analysis of the fair data of a solar energy power plant with SPSS
60-63Views:102The use of fossil energy sources greatly damages the environment. Moreover, the quantity of these energy sources is limited. Therefore, it is important to increase the share of renewable energy sources (solar, wind, water and biomass) in energy generation.
Huge amounts of energy (1100-1300 kWh/m2 per year) arrive at the earth from the sun, and are utilized in passive and active ways. One of the active applications is photovoltaic current production, in the course of which electricity is produced directly with PV – panels. This can be fed into a grid. At the University of Debrecen is a solar energy power plant from September 2005 in operation. The electricity performance of the incorporated PV-panels (Kyocera, Dunasolar, and Siemens) are 8.64 kW. The are of PV – panels is 110 square meter. With the aid of the data storage, the tension, current, temperature of the PV-panels, global radiation, air temperature, wind speed, wind direction and the achievement is measured by the ac network.
The effect of the shading on the performance of the PV – panels and the solar energy power plant is examined. The analysis and the graphic representation of the experiment results are carried out with SPSS per grief. We produced per grief. -
Changes in the Sulphur Content of Winter Wheat in a Field Experiment
85-88Views:79The use of superphosphate as P-containing fertiliser decreased in the last years in many countries in accordance with strict air pollution laws, and the S-deposition decreased from the atmosphere to the soil as well.
Winter wheat is the one of the S-demanding plants. Recently, the gradually increasing S absence endanger the formation of required average yield of winter wheat, and has bad effect on its quality.
We examined the effect of treatments on the sulphur-, nitrogen content and the N/S ratio of winter wheat in the whole upperground plant and in the grain and straw at harvest in a arable land sulphur fertilization experiment on brown forest soil (Agricultural Company of Felsőzsolca).
We analysed the samples from spring to harvesting, in the critical phenophases. In this study we discuss only the values from the stooling and stalking and the results of analysis of grain and straw in the harvest.
We experienced that the concentration of sulphur in the whole upperground parts of winter wheat showed increase to the end of vegetation independently of fertilization. The N/S ratio was between 8% and 12% in the beginning of the growth period in the whole upperground plant, while the ratio in the grain at harvest was between 13 and 14%. When we examined the whole upperground plant, stalk and leaf at stalking, we got the highest sulphur content in the leaf. Mostly the middle level sulphur fertilization dose (4 l/ha) increased the sulphur accumulation in the green plant. At total maturing, the greatest part of accumulated sulphur is in the grain, but then the effect of fertilization is less glaring. -
Cytochrome b diversity of Hungarian Botrytis cinerea strains
18-21Views:103In the mitochondrion of eukaryotes, cytochrome b is a component of respiratory chain complex III. Cytochrome b is encoded by the
cytochrome b (CYTB) gene located in the mitochondrial genome. The fungicidal activity of QoIs relies on their ability to inhibit mitochondrial respiration by binding at the so-called Qo site (the outer quinol-oxidation site) of the complex III. Since their introduction, QoIs (like azoxystrobin) have become essential components of plant disease control programs because of their wide-ranging efficacy against many agriculturally important fungal diseases like grey mould on various crops. QoI resistance primarily arises from a target-site-based mechanism involving mutations in the mitochondrial CYTB. As the management of grey mould is often dependent on chemicals, the rational design of control programs requires the information about the diversity of genes connected with resistance in field populations of the pathogen.
Monospore B. cinerea field isolates has been collected during 2008-2009 from different hosts in Hungary. PCR fragment length analysis
indicated the high frequency presence of type large intron in the isolates while in a few strains G143A substitution could also be detected.
These results indicated the heterogeneity of CYTB in the Hungarian B. cinerea populations, which possibly involve the heteroplasmy of this
mitochondrial gene, moreover indicates the existence op azoxystrobin resistant populations in Hungary.
This work was supported by NKFP-A2-2006/0017 grant. Erzsébet Fekete is a grantee of the János Bolyai Scholarship (BO/00519/09/8). -
Using research findings in precision maize production
227-231Views:215The effect of crop production factors on maize yield are examined on chernozem soil in a more than 30 year old long-term experiment on the Látókép Experiment Site of the Centre for Agricultural and Applied Economic Sciences of the University of Debrecen. The aim of research is to evaluate the effect of fertilisation, cultivation, plant number, genorype and irrigation. The analysis of the data in the database of the examined period makes it possible to evaluate the effect of maize yield, as well as that of the crop production factors and the crop year, while the correlations and interactions between these factors were also examined. During the examination of the cultivation treatments, it was concluded that the highest yield was obtained as a result of autumn ploughing, but its effect largely differs in the irrigated and the nonirrigated treatments. Based on our examinations, strip cultivation should be applied periodically (e.g. strip – strip – ploughing – loosening) in areas with favourable soil conditions free from compacted layers.
In years with smaller, average precipitation supply or when the precipitation was higher than average, higher plant numbers were more favourable. Under drier conditions, but especially in several consecutively dry years, a lower plant number can be recommended which is not higher than 60 thousand per hectare. In the case of favourable water supply, 70-80 thousand plants per hectare can be used. The yield increasing effect of fertilisation was significant in the case of both non-irrigated and irrigated conditions, but it was much more moderate in the non-irrigated treatment. The extent of weed coverage was significantly affected by the previous crop. In the case of a favourable previous crop (wheat), the weed coverage was significantly lower than after an unfavourable previous crop (maize). In the case of the same previous crop (maize), the extent of weed coverage was mostly determined by the crop year and the extent of precipitation supply. Irrigation is not enough in itself, because if it was not accompanied by intensive nutrient management, yields started to decline.
The results of researhc, development and innovation contributed to the technological method which makes it possible to apply locally adjusted sowing seed, fertiliser and pesticide in a differentiated way, as well as to change the method of operations within the given plot. -
Texture analysis as a method for grape berry characterization
Views:217The marketability of table grapes is mainly characterized by berry size, color, taste and texture. Mechanical measurements of table grape berries could provide objective information on the textural qualities of grape berries. In addition, this method might be suitable to study the effects of phytotechnical methods (such as girdling) on table grape quality. The aim of this preliminary work was to demonstrate how instrumental testing could be used to examine the effects of girdling on berry grape texture and define the textural characteristics of table grape berries. Cane girdling was carried out at veraison in two table grape varieties. Texture analysis was performed several times during the maturity. Besides this examination another five varieties were analyzed to assess their berry mechanical properties. Double compression test was used to determine berry hardness and its derived parameters. Puncture test was applied to evaluate skin hardness, skin elasticity and skin break energy. Skin thickness was also investigated. Berry hardness, skin hardness and skin thickness of the girdled grapevines were significantly affected by this technique. Most of the textural parameters showed differences among the seven cultivars.
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Comparative analysis of soil analysing datas on different sempling-plots
85-90Views:69Hibrid maize is cultivated on larger plots, therefore the sown areas of hibrid maize are heterogeneous from a pedology aspect. Heterogenity causes problems during tasseling, chemical plant protection and harvest. The heterogenity of sown areas can be compensated by fertilization which is based on soil analysis. We carried out research into change of the soil on four soil types from 1987 to 2005.
There were no significant changes in pH, hydroiodic acidity, CaCO3-content, humus-content on meadow chernozem soil. We detected equalization of salin content in the examined soil layers. There were no significant changes in the measured values on chernozem meadow soil and solonetz meadow soil in 2005. We discoverd equalization of saline content on chernozem meadow soil, but the changes were not as obvious as the changes on meadow chernozem soil. We found salinization in the 30-60 cm soil layer on type meadow soil that may be due to water movement. -
Comparison of RAPD and AFLP Analysis in Some Maize (Zea mays L.) Lines and Hybrids
3-7Views:96The use of molecular markers to enhance plant breeding efforts is being widely studied. DNA-based fingerprinting technologies (RAPD and AFLP) have proven useful in genetic similarity studies. We estimated different maize (Zea mays L.) inbred lines and hybrids originated from mutant ones based on their genetic differences.
We carried out RAPD analysis with different primers and the 707 (CCCAACACCC) and 792 (CAACCCACAC) primers with 50% similarities provided quite good DNA fragments. By applying the DNA based-AFLP technique, we had very dense DNA fingerprinting. We differentiated 15-32 polymorphic bands, the highest number of bands were found in P-T/H-CA (32). AFLP seems to be the more efficient method of comparing genetic similarities/differences among different genotypes. -
Analysis of the photosynthetic parameters, the yield and the quality of winter wheat
101-106Views:141The environmental adaptability of crop production is basically determined by the selection of biological background (plant species and
varieties) suitable for the region and the site. The aim of our work is to parametrize the plant assimilation, its intensity, dynamics and the
most important characteristics and the relationships to the quality in winter wheat trials. The measurements were carried out at the research
site of the University of Debrecen in small parcel experiments. We measured the leaf net CO2 assimilation rate, stomatal conductance,
intercellular CO2 level, the transpiration, the leaf temperature and the air temperature by the LICOR LI-6400 portable photosynthesis
system in field trials on the nutrient supply. The soil of the experimental area is calciferous chernozem with favorable water regime.
We have examined the photosynthetic activity, the productivity and yield stability of winter wheat varieties. We have compared the yield
results, at similar agrotechnical conditions in seven cropyears. We also determined the quality parameters of the winter wheat varieties.
Then we valued the yield stability of genotypes with the help of analysis of variance and linear regression equations. We have defined the
connections between assimilation parameters, the yield stability and quality parameters of wheat varieties. -
Statistical comparison of coverage data of disturbed habitats in the Hajdúság
171-178Views:77Between 2002 and 2006 we made the coenological survey of five disturbed habitats marked as grasslands. With our coenological examinations and the statistical analyses we wanted to make the detailed botanical survey of the given five habitats in order to verify that the maintenance of habitats amongst agricultural lands – and considered as less valuable – is of high importance and necessary from an environmental point of view, since these habitats are often living and feeding areas of many rare and/or protected plant- and animal species.
As a result of the statistical analyses we have pointed out that number of species in case of all the five habitats extreme fluctuation characterizes the statistical universe. As regards the
average of the coverage it is the highest in case of the third habitat (degraded Puccinellia grassland), and the coefficient of variation shows homogenity as well. In examining the Shannon-value the average is the highest in case of the second habitat (Alopecurus meadow), and the statistical dispersion is the smallest. The coefficient of variation shows medium variability. The median of evenness is the lower in case of the third habitat (degraded
Puccinellia meadow) and the statistical is the highest here as well.
We have done the Hierarchical and the K-Means Cluster Analyses for the 21 plant associations of the five habitats. Both cluster analyses put the same associations into the same cluster, so
one can state that the associations in each cluster are different from the associations of the other ones according to the coverage data of the plant families. -
Effects of production factors on the yield and yield component of winter wheat
26-31Views:80The effect of major production factors (forecrop, fertilisation, irrigation, soil cultivation and soil preparation) on the yield components and yield of winter wheat were studied in a long-term experiment set up at the Látókép Experimental Nursery of the Agricultural Sciences Centre of the University of Debrecen. The results of regression analysis led to the following conclusions:
• In our experiments in 2000, after using maize as a forecrop –based on the results of analysis of regression – fertilisation determined the yield.
• After using pea as a forecrop, a N50 P35 K40 kg/ha fertiliser rate led to an economical increase in the yield of winter wheat.
• None of the determinative yield components varied significantly for winter wheat produced after using pea as a forecrop.
• There is a closed, significant correlation between plant height, spike length, plant and spike mass, the number of spikelets and grains per spike after using maize as forecrop. The thousand grain mass is different from the other yield components, because it is not part of the relation system of
those yield components.
• The increased yield of winter wheat after maize has been used as a forecrop is due to the positive change in grain number per spike yield component. -
Comparative analysis of sample preparation methods to determine the concentration of arsenic in soil- and plant-samples
167-170Views:151Arsenic contamination of the fields and groundwater is a global problem. Alföld is the most affected area in Hungary. Irrigation witharsenic contaminated water, and crop production on the contaminated soil can cause a food safety problem, because arsenic is easy taken up by the cell of the plant roots. To prevent this, very important to monitoring the arsenic content of soils and plants. Inductively coupled plasma mass spectrometry (ICP-MS) is a fast, easy method to determined the concentration of minerals in the case of plant and soil samples The analytical methods can give reliable, results if the analytical process, including the sample preparation method, is the best. The objective of this study was to compare 3 type of sample preparation method which was dry ashing, wet digestion in open system, and microwave digestion. As a result of our experiement shows the microwave digestion is the appropriate method to determined the arsenic content of soil samples. In the case of plant samples we can use wet digestion in open system or microwave digestion as a samle preparation method.
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The scientific background of competitive maize production
33-46Views:294The effect and interaction of crop production factors on maize yield has been examined for nearly 40 years at the Látókép Experiment Site of the University of Debrecen in a long-term field experiment that is unique and acknowledged in Europe. The research aim is to evaluate the effect of fertilisation, tillage, genotype, sowing, plant density, crop protection and irrigation. The analysis of the database of the examined period makes it possible to evaluate maize yield, as well as the effect of crop production factors and crop year, as well as the interaction between these factors.
Based on the different tillage methods, it can be concluded that autumn ploughing provides the highest yield, but its effect significantly differed in irrigated and non-irrigated treatments. The periodical application of strip tillage is justified in areas with favourable soil conditions and free from compated layers (e.g. strip – strip – ploughing – loosening). Under conditions prone to drought, but especially in several consecutive years, a plant density of 70–80 thousand crops per hectare should be used in the case of favourable precipitation supply, but 60 thousand crops per hectare should not be exceeded in dry crop years. The yield increasing effect of fertilisation is significant both under non-irrigated and irrigated conditions, but it is much more moderate in the non-irrigated treatment.
Selecting the optimum sowing date is of key importance from the aspect of maize yield, especially in dry crop years. Irrigation is not enough in itself without intensive nutrient management, since it may lead to yield decrease.
The results of research, development and innovation, which are based on the performed long-term field experiment, contribute to the production technological methods which provide an opportunity to use sowing seeds, fertilisers and pesticides in a regionally tailored and differentiated way, adapted to the specific needs of the given plot, as well as to plan each operation and to implement precision maize production.
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Changes of relative chlorophyll content at maize smut inoculated hybrids
55-58Views:249The leaf chlorophyll content analysis is important for several reasons. The natural or anthropogenic stressors directly effect on the chlorophyll content. Through the measurement of the chlorophyll content it is possible to obtain data concerning the physiological status of the plant, moreover the chlorophyll content is closely related to the nitrogen content, so it is linked to photosynthesis and the photosynthetic activity which determine biomass production.
One of the most common symptoms of plant diseases is the larger and smaller interveinal chlorotic areas. These might be local, or expand to the whole plant. There are multiply reasons of chlorosis such as reduction of chlorophyll content, unfavorable effects on the chlorophyll content,disorders regarding function of chloroplasts or ultimately destruction of the chloroplasts. Although such a chlorotic deviancy can contribute to significant losses in photosynthesis; however the underperformance photosynthesis of the sick plants is a more complex process.
As we unambiguously experienced during our investigations on common smut that the infected maize plants most common accompanying symptom was chlorosis on the leaves, so it is especially important to examine how the infection influenced on the chlorophyll content of different hybrids. -
Molybdenum - accumulation dynamics of cereals on calcareous chernozem soil
81-85Views:123This work is about the molybdenum-accumulation of cereals analyzing soil and plant samples from a field experiment set in
Nagyhörcsök by Kádár et al. in 1991.
In this long-term field experiment different levels of soil contamination conditions are simulated. Soil and plant samples were collected
from the experiment station to study the behaviour of molybdenum.
In this report results of maize, winter wheat, winter barley and soil analysis are presented. The conclusions are as follows:
– Analysing soil samples from 1991 we have found that roughly half of the molybdenum dose applied is in the form of NH4-acetate+EDTA soluble
– Comparing element content of grain and leaf samples we have experienced that molybdenum accumulation is more considerable in the vegetative plant parts
– Winter wheat accumulated less molybdenum then maize in its vegetative parts. Comparing molybdenum content of winter wheat to winter barley we found that the concentration of the element in wheat was lower by half than in the winter barley. It seemed that molybdenum accumulated to the least degree in winter wheat. -
Study of some physiologic properties of different genotype sweet corn hybrids
105-110Views:99The effect of nutrient-supply (control, N120+PK) and two different genotypes on the physiologic properties of sweet corn has been investigated in the crop-year of 2011 on chernozem soil in the Hajdúság region. The experiments were carried out at the Experimental Station of the University of Debrecen in Debrecen-Látókép. The experiment was sewn in two different sowing times: the 21st April can be considered as an early, while the 19th May as a late sowing time. The two involved hybrids were Jumbo and Enterprise. The applied plant density was 65 000 plants per hectare.
Our aim with this experiment was to study the plant production, just as the main affecting factors of its development and dynamics, like nutrient-supply and genotypes. We aimed to study and analyse the relationships between these factors and plant production. In this study following parameters were measured and calculated: photosynthetic activity, chlorophyll-content (SPAD-value), leaf area index (LAI) and leaf area duration (LAD). Regarding the analysis of photosynthetic activity values no obvious relationship between the measured values and the applied hybrids, just as nutrient-supply has been revealed.
Analysing the SPAD-values it can be stated that the chlorophyll-content of the measured leaves showed an increasing tendency due to the nutrient-supply. The highest values have been measured in the intensive cob development phase of the early sowing time plots.
Regarding the LAI-values we have found significant differences between the fertilizer treatments in both sowing time treatments. In case of the leaf area duration values – that is derived from the LAI values – nutrient-supply has positively affected the duration of the assimilation area. -
Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:294The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.
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Effects of bioactive plant extracts on immune-related gene expression of common carp (Cyprinus carpio)
49-56Views:271In recent years, intensive fish farming has led to an outbreak of several diseases, and the health status of fish can affect the economy of aquaculture. Since fish health and intestinal health are in correlation, it may also have an impact on immunity. Accordingly, many natural feed additives are being used to improve immune functions. In our study, carotenoids, oligosaccharides, and anthocyanins were applied at 1 m/m% in feed to investigate their effects on cytokines, such as interleukin-1β (IL-1β), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interferon regulatory factor-1 (IRF-1) in spleen and mid-intestine of 6 months old carp. Gene expression analysis was carried out to examine IL-1β, IL-8, TNF-α, and IRF-1 mRNA levels in fish spleen and mid-intestine. The gene expression level of pro-inflammatory IL-1β decreased in the mid-intestine of carotenoid-fed carp compared to anthocyanin supplemented group, but the effects of the bioactive plant extracts were not observed on the examined cytokines compared to control fish.
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In vitro analysis of the effect of ragweed extract against Monilinia laxa
117-120Views:109Nowadays in Hungary nearly 5 million hectares of agricultural area was infected with ragweed (Ambrosia artemisiifolia). According to the public opinion the ragweed is a weed. From agricultural and public health point of view it is exceptionally dangerous plant. As it contains a number of useful active ingredients, based on this the ragweed is consider a medicinal plant. Our goal was to present that the ragweed contains antifungal active substances as well. In the experiments we used the pre-flowering plants with roots and we extracted the biological active components of dried plant. We tested the biological activity of the extracts against Monilinia laxa in vitro. We related based on our examination that ragweed contains biologically active agents, by which it is hampered the reproduction of the Monilinia laxa.
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Sensitivity of methods for estimating reference evapotranspiration
51-56Views:147The knowledge of the evapotranspiration of natural ecosystems and plant populations is of fundamental importance in several branches of science, research topics and practical uses. Nevertheless, the harmonization of the large number of methods and the changing user needs often causes problems. Sensitivity analysis of 10 ET0 estimation models and model variants was performed. Magnitude of the obtained outputs and the changes triggered by each atmospheric parameter were evaluated. The objective of the analyses was to get to know the sensitivity of the different models and to select the most accurate and the most suitable ones for adaptation to local circumstances. Therefore, it becomes possible to achieve as high accuracy as possible in applications which need ET0 estimation.
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The Role and Significance of Soil Analyses in Plant Nutrition and Environmental Protection
3-8Views:84Hungary has a rich history of soil analyses and soil mapping. Our main tasks today are the preservation of soil fertility as well as balancing the goals of production and environmental protection. The main requirement of agricultural production is to adapt to ecological and economic conditions.
In a series of consultative meetings in the past seven years, representatives from Central and Eastern Europe have analyzed nutrient management practices in their respective countries. According to a joint memorandum agreed upon in 2000, in the countries awaiting accession, the quantity of nutrients used per hectare is considerably smaller than the Western-European usage targeted through special subsidies. The current low nutrient usage contradicts the principles of sustainability and that of the efficient use of resources, jeopardizing soil fertility.
In Hungary, the use of inorganic fertilizers underwent a dynamic development, which manifested itself in an almost tenfold usage growth between 1960 and 1985. This growth slowed down somewhat between 1985 and 1990 and then reduced dramatically after 1990, reaching record lows at the usage levels of the 60s. The nutrient supply has had a negative balance for the last 15 years.
The increasing and then decreasing usage trends can equally be detected in the domestic yield averages of wheat and corn as well as in the nutrient supply of soils. Yields were the largest when usage levels were the highest, and decreased thereafter. Draughts have also contributed to smaller yields. The dramatic decrease in the use of inorganic fertilizers when adequate organic fertilizers are lacking endangers our soils’ fertility.
About 50% of soils in Hungary are acidic. Acidity is mostly determined by soil formation, but especially on soils with a low buffering capacity, this acidity may intensify due to inorganic fertilizers. Sustainable agriculture requires the chemical improvement of acidic soils. According to their y1 values, the majority of our acidic soils need to be improved. This chemical soil remediation is required in 15% of the acidic soils, while it’s recommended for another 20% of these soils.
Results of the analyses conducted in the framework of the soil-monitoring system set up in Hungary in 1992 show that in 95% of the analyzed samples, the toxic element content is below the allowable limit. Cultivated areas are not contaminated; toxicity above the legal level was found only in specific high-risk sampling areas: in the vicinity of industry, due to local overload. The basic principle of sustainable agriculture is to preserve soil fertility without undue strain on the environment. The intensity of the production needs to be considered according to the conditions of the site; i.e.; nutrient management needs to be site-specific. It is recommended to differentiate three types of cultivated land in terms of environmental sensitivity: areas with favorable conditions, endangered areas, and protected areas, and then to adopt nutrient management practices accordingly. To meet all the above-mentioned goals is impossible without systematic soil analysis. Tests conducted by the national monitoring system cannot replace regular field measurements. -
Plant growth analysis of wheat populations in a long-term field-experiment
147-151Views:134The experiments were conducted as part of the long-term trial adjusted, in triculture (pea-wheat-corn) and biculture (wheat-corn), at three nutrition levels, with the use of one crop protection technologie (conventional) at the Látókép Research Site of the Centre of Agricultural Scienses, University of Debrecen, on a chernozem soil. The wheat variety used in the long-term trial was GK Csillag, which was sown at 5,8 million germs/ha.
The effect of pre-crops and nutrient-supply levels on some growth-parameters (LAI, HI, LAD), just as SPAD-values and yield amounts of winter wheat has been investigated in this experiment. We tried to find out the extent of relationship between the different parameters, so we determined the relationships between different nutrient-supply levels, yield amounts, LAI- SPAD- and LAD-values – measured in the crop-year of 2010–2011 in different crop rotation systems – by using correlation analysis. It has been stated both in case of bi- and tri-culture crop-rotation systems that different fertilizer dosages had significantly affected the leaf area index dynamics and its maximal value, and that increasing N+PK fertilization has
significantly increased the duration of leaves, as well. The highest SPAD-values were measured during the flowering and grain filling stages. However, we haven’t revealed significant differences between all fertilizer treatments. In case of the bi-culture crop-rotation system harvest index values showed an increasing tendency parallel to the increasing nutrient-supply levels, while in case of the tri-culture system this tendency was rather decreasing. However, these differences were
not significant. Parallel to the increasing fertilizer dosages yield results were increased in a significant extent. At the same nutrient supply-levels 2088–4615 kg ha-1 higher yields were measured in the tri-culture than in the bi-culture system. The correlation analyses have confirmed that all of the investigated parameters (yield amount, LAI, SPAD, LAD) had almost in all cases close positive correlation to the nutrient-supply level and the yield amount in both crop-rotation systems. These results have confirmed that the leaf area, the leaf duration, the SPAD-values and the fertilization have altogether resulted in the production of maximum grain yields. -
Qualitative detection of genetically modified organisms in plant samples
309-313Views:124We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.
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Analysis of maize and sunflower plants treated by molybdenum in rhizobox experiment
11-14Views:215In this study, maize (Zea mays L. cv. Norma SC) and sunflower (Helianthus annuus L. cv Arena PR) seedlings treated by molybdenum (Mo) that were cultivated in special plant growth boxes, known as rhizoboxes. During our research we tried to examine whether increasing molybdenum (Mo) concentration effects on the dry mass and absorption of some elements (molybdenum, iron, sulphur) of shoots and roots of experimental plants.
In this experiment calcareous chernozem soil was used and Mo was supplemented into the soil as ammonium molybdate [(NH4)6Mo7O24.4H2O] in four different concentrations as follow: 0 (control), 30, 90 and 270 mg kg-1.
In this study we found that molybdenum in small amount (30 mg kg-1) affected positively on growth of maize and sunflower seedlings, however, further increase of Mo content reduced the dry weights of shoots and roots. In case of maize the highest Mo treatment (270 mg kg-1) and in case of sunflower 90 mg kg-1 treatment caused a significant reduction in plant growth.
In addition, we observed that molybdenum levels in seedling were significantly elevated with increasing the concentration of molybdenum treatment in comparison with control but the applied molybdenum treatments did not affect iron and sulphur concentration in all cases significantly.
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Nitrogen Content of Hungarian Soils and Nitrogen Fertilization
51-61Views:95For crop production and agricultural production, the most important natural resource is the soil that can optionally renew. Paralelly with this, soil plays a major role in the geological and biological cycle of elements. As a result of the big (geological) and small (biological) element cycles, the elements and combines neccessary for organisms can accumulate in the soil creating suitable living conditions for plants and other organisms. Soil is a heterogenous system both horizontally and vertically, and soil constituents show great variety in all the three dimensions, in addition, most of the parameters can also change between two examination dates. When talking about the factors influencing plant production, one should take into account this variation and heterogenity in time and space. When making fertilization recommendations, these factors should all be considered. In any consultation system, most of the mistakes and errors made are due to the unsatisfying soil testing and the negligence of soil heterogenity. In the practice of fertilization the biggest mistake is the improper soil sampling, then comes the methodical mistake of soil testing, which is followed by the inaccuracy of instrumental analysis and the subjectivity of result evaluation, but the latter two are negligible compared to the others. Under normal, i.e. production conditions, the quantity and distribution of nutrients in the soil are greatly dependent upon the applied technology, the amount and form of the applied natural and artificial fertilizers and the quality of fertilization.
Fertilization recommendations are needed because in the layer which is accessible for plant roots only a part of the nutrient content is available for plants in a specific production cycle. An illustration of this is that though the upper 1 m layer of an average chernozem soil contains more than 5000 kg N, 12000 kg K2O and 1500 kg P2O5 (form of expression mostly used in Hungary), the application of fertilizer doses which are just fractions of these quantities is essential. This is due to the fact that the available amount of the total nutrient content depends from the quality of soil, the environmental factors (the physical and chemical qualities of the soil) and the specific nutrient’s qualities (solubility, adsorption). Knowledge of these processes and the examination of the factors influencing the actual nutrient content are vital for working out a fertilization practice, which does not put more strain on the environment than neccessary.All of the above mentioned should be considered when applying inputs in the fields. In a well-functioning practice that considers the economic and environmental conditions (unfortunately the present production and economic conditions do not enable an appropriate level and degree), three nutrients are supplemented generally (and were supplemented in the last decades): nitrogen, phosphorus, potassium.
Studying the nutrient balance of the Hungarian field production’s last hundred years, we can draw some interesting conclusions.
The nutrient balance became positive for nitrogen and potassium in the second half of the 1960’s, while for phosphorus it was positive from the first half of the 1960’s and this period lasted until the end of the 1980’s.
Neither before the 1960’s, nor since the 1990’s has the amount of nutrients supplemented in a specific year reached the amount of the nutrient uptake of the same year.