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Evaluation of the microbial soil quality indicators in agricultural soils from Crisurilor Plain
79-80Views:126The researches were carried out in 2010 and 2011 on the haplic luvisol cultivated in three variant such as: pasture, cropland, and orchards. Based on the total number of microorganisms monitored in the haplic luvisol (aerobic mesophilic heterotrophs, yeast and mould, Actinomycetes, nitrogen fixing bacteria and nitrifying bacteria) was calculated the bacterial indicators of soil quality (BISQ) and was appreciate the bacterial potential of the haplic luvisol. Seasonal analyses were carried out, and annual BISQs have been calculated too. The values of the bacterial indicators of the haplic luvisol in different cultivation conditions (pasture, cropland and orchards) indicate a high density of the bacterial groups in 2010 and 2011. In 2010 the values of the BISQs are included between 3.617 (minimum in autumn, in pasture) and 5.458 (maximum, in spring, in pasture). In 2011, the minimum value (3.622) was registered in pasture, in autumn, and the maximum value (4.851) in the haplic luvisol cultivated with maize, in spring. In 2010 and 2011, based on the bacterial indicators of the soil quality values, on the first position is the cropland (4.750, 4.721) followed by orchards (4.615, 3.985) and pasture (4.537, 3.758).
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Challenges and agroecological approaches in crop production
75-89Views:178Never has the need been greater for an ecosystem approach to agriculture. As our global population exceeds 9 billion in the next 30 years, with a concomitant demand for agricultural products, ever more pressure will be placed on our agricultural systems. Meanwhile, climate change is altering the ecological settings in which agriculture is practiced, demanding adaptation. Knowledge generated by long-term research will help to address one of the grand challenges of our time: how to meet sustainably the growing world demand for agricultural products – in a way that minimizes environmental harm and enhances the delivery of a diverse array of ecosystem services.
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Agronomical and economic evaluation of various cultivation systems on meadow soil
103-106Views:122The requirements and objectives of cultivation are in constant change. There are different cultivation aims if the objective is soil protection, the prevention of its moisture content or on areas with different precipitation supply or production site endowments. Based on the experimental database of the Institute for Land Utilisation, Regional Development and Technology of the University of Debrecen, Centre for Agricultural and Applied Economic Sciences and the KITE Plc., the various cultivation systems in Hajdú-Bihar country were examined with maize as indicator plant. The sample area can be found in the outskirts of Biharnagybajom on meadow soil. On the examined plot, spring strip basic cultivation, loosening and autumn ploughing were applied on 15-15-15 ha, respectively. At the time of taking undisturbed soil samples, soil conductivity measurements were also performed with a Penetronik penetrometer. Undisturbed soil samples were taken from each treatment before sowing (on 5th April 2012). The yield obtained in the strip cultivation treatment increased that of the ploughing and the loosening technology. The economic indexes are the most favourable in the strip cultivation.
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Impact assesment of soil conditioners on a high clay content soil
137-141Views:170Our measurements were done in a soil conditioner experiment started in 2014 which was set in conventional tillage system at the Karcag Research Institute where a soil conditioner was used from 2010. Effect of two different soil conditioners on compaction, moisture content of the soil and on CO2-emission was studied. Measurements were done after sowing of maize and millet, and on stubble after harvesting. It can be established that less degree of compaction was characteristic to the soil of the plots treated for several years with the soil conditioner during the vegetation period than in case of untreated plots. Higher CO2-emission values were observed on the plots treated for several years than on the control plots. This effect can’t be established in case of soil conditioners used for first time in this year.
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A dual infection of two microscopic fungi on common milkweed (Asclepias syriaca) in Hajdúság region (East-Hungary)
189-195Views:195Common milkweed (Asclepias syriaca L.) is one of the most noxious and invasive weed species in Hungary. A. syriaca invades arable lands, horticultural and forestry plantations, natural and semi-natural habitats too. In cases of field crops it can cause considerable yield losses mostly in maize (2–10%), soybean (12–32%) and sorghum (4–29%), but only with high rate of coverage. It can also increase these problems that the common milkweed can be serve as reservoir and host for viruses, other pathogens and pests.
Because of the importance of common milkweed and in spite of demand to develop effective biological control, until now has not been developed a proper control program against A. syriaca. The aim of our research was to identify the necrotrophic fungal pathogens, which were involved in notable disease occurrence on this weed in different parts of Hajdúság region of Eastern-Hungary in 2016.
To the isolation of fungi from leaves and their identification were based on morphological colony characters on potato dextrose agar (PDA) and Sabouraud dextrose agar (SDA). To the description of conidia features were used PDA for Alternaria and synthetic low-nutrient agar (SNA) for Fusarium species, respectively. The examination of axenic cultures revealed that the fungi isolated from the leaves of common milkweed were Fusarium sporotrichioides and Alternaria alternata. -
Evaluation of critical factors determining the profitability of sweet maize
97-102Views:137The main goal of this analysis was to determine, whether the production of sweet corn competitive is from the point of view of profitability and identify the main factors determining profitability. The hypothesis of this research was that sweet corn production is profitable and output factors (yields, selling prices) affect most significantly the profitability. The total costs of production with irrigation are 560 000 HUF ha-1. Average yield is 18 t ha-1, while selling prices on average of the last 5 years were about 38 000 HUF t-1. The realisable profit in the sector was 248 828 HUF ha-1. Direct cost-related profitability was 48.6% and profit level 26%. Elasticity analysis pointed out that the changes of selling prices and yields affect profitability. The critical value shows the turning point of profitability, which was at the yield of 13.82 t ha-1. It can be concluded, the hypothesis was true, because sweet corn production was profitable compared to other field crops.
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Mass occurrence of a Phoma-like fungus on common ragweed (Ambrosia artemisiifolia) in Hajdúság region, East Hungary
55-60Views:233Common ragweed (Ambrosia artemisiifolia L.) is one of the most important, allergenic weed species in Hungary. A. artemisiifolia invades both a broad range of often disturbed areas (brownfields) and either undisturbed ones like waste lands, roadsides, riverbanks and railway tracks. In field crops it can cause considerable yield losses mainly in sunflower, maize, cereals and soybean. In Hungary many inhabitants suffer from allergy caused by Ambrosia pollen which results a serious human-health risk. The aim of the control is to prevent flowering and seed propagation of A. artemisiifolia. Until now the occurrence of numerous pathogenic fungi which attack common ragweed plants have been identified in Hungary, however there is not yet available biological weed control program because of shortage in acceptable effectiveness, and endangering cultural plant species. During our weed surveys in the region of Hajdúság (East-Hungary) we found numerous common ragweed plants showing heavy necrotic lesions on leaves and stems. The objective of this study was to identify the fungus which was isolated from diseased tissues of common ragweed (A. artemisiifolia). The identification of fungus based on morphological characters of colonies and features of conidia and chlamydospores developed on malt extract agar (MEA) plates. After examination of axenic cultures we revealed that the fungus isolated from the leaves ands stems of common ragweed was a Phoma-like species.
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The Examination of the Year Effect Based on Cumulative Temperature and Rainfall Curve
70-77Views:93In order to get to know the numerical differencies of favourable and unfavourable weather conditions, our research lean to 29-years-data (1961-1989) of maize yield of productive area of Hajdúság from which we chose two low (1964, 1976) and two high (1969, 1975) yield yerars. Choosing the years we fitted a linear trend to the time series and the valued data was divided by the realised crop. From the cumlative temperature- and rainfall data calculatd curve concern the period between 01. April and 01. September. The basis was the cumulative value of many years daily average value. The temperature- and rainfall curve of favourable and unfavourable yield with the many years average data was represented on the same scale. In order to numericate the differencies we count the differencies monthly frequency. Based on it became sunder the feature of years. The method used by us ensure possibility to separate the temporal types. It was found that informing the yield the rainfall has main role in this productive area and the temperature has only second role.
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Host plant preference of Metcalfa pruinosa (Say, 1830) (Hemiptera: Flatidae) in the north of Hungary
84-95Views:251Citrus flatid planthopper, a native insect to North America had for a long time a scarce economic importance there. However, being polyphagous made little damage on citrus trees and some ornamentals. In 1979 it was introduced to Italy where it established and spread quickly. It is now an invasive alien species continually spreading in South and Central Europe causing considerable damage in fruit crops and various ornamentals. Present study shows the results of a series of observations carried out from 2011 to 2015 at a number of habitats in north of Hungary. The pest could be found at each habitat but the hedge, the tree row, the gardens and the orchard/vineyard were the most infested. Frequency and population density of Metcalfa pruinosa were considerable on Asteraceae, Cannabaceae, Fabaceae, Juglandaceae, Lamiaceae, Rosaceae and Sapindaceae. Typical vegetation could be functionally classified as ornamental plants, trees/shrubs, fruit plants, weeds and feral plants. Feral plants – some of them also invasive alien species – were found at each habitat. Plant species native to America were among them the most populated. As the hedgerows were neglected, and most gardens, orchards and vineyards abandoned, these are excellent conditions for the quick and long-lasting establishment of the pest as well as they may be reservoirs to infest cultivated fruit crops and ornamentals. The hedgerow was situated along a railway line. The length of similar hedges can be merely in Pest county several hundred km, which means M. pruinosa has plenty of opportunity for spreading along the railway and infest agricultural and ornamental cultures. On the surveyed alfalfa and maize fields, accidentally very few nymphs and adults were observed. Although, the population density of M. pruinosa was considerable on many hostplants, economic damage or yield losses could not be detected. Economic or significant damage was observed only on roses, raspberries and stinging nettle. This later is cultivated in Germany and Finland. The applied horticultural oil was efficient.
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Virtual Appliances for geospatial data management and processing in the Integrated Land Management System (ILMS)
59-62Views:112Virtualization is increasingly taking on a key role in various system architectures which follow new platform concepts like Software as a Service (SaaS). This trend addresses more instant and short-term environments and comes with new methods and strategies for the distribution of mainly complex application stacks not only in large IT infrastructures. The paper presents how a so called Virtual Appliance can be set up in order to operate in virtual server environments using hypervisor software like Oracle Virtual-Box. Using the example of two server-side components within the Integrated Land Management System (ILMS), it will be shown that the use of state-of-the-art methods, standardized tools and interfaces on servers enables different aspects of environmental system management, analysis and planning.
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Comparison of Pálfai’s drought index and the Normalised Precipitation Index in the North Great Plain region
59-64Views:229Agriculture has always been an important role in economy, food supplies, sustainability of society and creation of job opportunities in Hungary. Our country has resource-related strength of agriculture, because we have more than 4.5 million ha for agricultural production. Agricultural production can be influenced by several factors, including climate, hydrology, soil conditions and antropogenic impacts. Climate determines the quality and quantity of the crop yields. The climate conditions in Hungary are variable and it shows spatial and temporal extremes. As a result of this, drought have become more frequent in our country (2003, 2007, 2009, 2012), which is reflected in the decline in yields as well. In the present study, Pálfai's Drought Index (PAI) and the Standardized Precipitation Index (SPI) were compared 2003–2012 in Debrecen. The temperature and precipitation data were calculated from data provided by a local meteorological station to work out PAI, while the SPI-3 index values were downloaded from the database of the European Drought Observatory. This allowed to drought assessment in a local and regional scale. Our study was supplemented with SPI-3, soil moisture anomalies, PAI and yields of wheat (Triticum aestivum L.) and maize (Zea mays L.) to evaluating the impact of drought on agriculture.
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Allelopathic effects of water extracts of pieces of wooly cupgrass (Eriochloa villosa) on the seedlings of field crops
167-170Views:182In Hungary, the woolly cupgrass (Eriochloa villosa [Thunb.] Kunth) endanger row crops (i.e. corn, sunflower). Its fast spreading based on some reason viz. long-lasting emergence, reduced sensitivity to many kinds of herbicides, vigorous competitional ability and fast initial growth. Allelopathy, ability of many plant species to produce one or more biochemicals wgich is used tocompete with each others. In this experiment we examined, whether the woolly cupgrass possesses allelopathy, and if so, how influences on the development of cultured crops like maize, sunflower and lettuce.
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Soil Biological Activity within Integrated and Ecological Management of Soil
47-52Views:88The effects of the integrated (IS) and ecological (ES) management of soil on chosen parameters of soil biological activity were investigated in the period 1999-2000. The following characteristics were determined: biomass of microorganisms (Cmic), dehydrogenase activity (DHA), an amount of potentially mineralizable nitrogen (Nbiol), and nitrification intensity. Soil samples were collected from a stationary field experiment established in 1990 on gley brown soil at the Experimental Station of Slovak Agricultural University, Nitra. For each field with a different crop rotations two fertilization treatments were selected: (a) no fertilization and (b) use of manure for silage maize and, within IS, also mineral fertilizers. There was a statistically significant difference at α = 0.05 in the amount of biologically released nitrogen (Nbiol) between both systems and in the nitrification intensity in favour of ES. A higher amount of microbial biomass (Cmic) was noted for ES but without statistical significance. Cultivated crops and the timing of soil sampling were found to have the greatest effect on all the parameters observed in individual experimental years and within the two systems of soil management.
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Examination of the Nutrient and Water Utilization of Different Corn Genotypes in the Hajdúság
55-59Views:157The research was set up on chernozem soil at the Látókép MÉK research area of the University of Debrecen in Hungary. We examined the following factors of the hybrid P9494, P9578, PR37N01 and PR37M81 in 2013.Yield, yield production per 1 kg fertilizer, water utilization and nutrient reaction. We found that the best yield results were achieved at level N120+PK in case of hybrid P9494 (17 132 kg ha-1) P9578 (16 838 kg ha-1) and PR37N01 (17 476 kg ha-1) and at level N150+PK for hybrid PR37M81 (16 754 kg ha-1). Results of yield per 1 kg NPK studies indicate that the most intense yield growth occurred at level N30+PK compared to the control treatment. This means that yield production per 1 kg NPK was 39.2 kg kg-1 in the case of hybrid P9494, 54.2 kg kg-1 in the case of P9578, 17.6 kg kg-1 for PR37N01 and 44.2 kg kg-1 in the case of PR37M81. After comparing corn hybrids’ water utilization, our conclusion was that the control treatment achieved the worst results in the case of each hybrid (P9494: 20.8 kg mm-1, P9578: 21.0 kg mm-1, PR37N01: 26.2 kg mm-1, PR37M81: 19.5 kg mm-1). For hybrid P9494, P9578 and P37N01, the best water utilization results were measured at level N120+PK (31.5 kg mm-1, 31,0 kg mm-1 and 32.1 kg mm-1), while PR37M81 reached the highest values at level N150+PK (30.8 kg mm-1). We determined the hybrids’ nutrient reaction and its optimum fertilizer values. The best nutrient reaction results were achieved by hybrid PR37N01, while that of hybrid PR37M81 proved to be the weakest.
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Technological development of sustainable maize production and its effect on yield stability
379-388Views:204In 2015 and 2016, we examined the effect of NPK nutrients, sowing date and plant density on yield on typical meadow soil. The amount of precipitation was 282.0 mm in 2015 (January–September), 706.0 mm in 2016 and the 30-year averageis 445.8 mm.Agrotechnical factors:– Experiment a)5 Dow AgroSciences hybrid with three sowing dates and three plant densities– Experiment b)In 2015 eight, in 2016 ten hybrids with different genetic characteristics and growing seasons, with control (without fertilization), N80+PK and N160+PKtreatments, five plant densities (50–90 thousand) with 10 thousand plants difference between the different densities.
In a drought year, we reached the higher yield in the earlier sowing date and with the lower lower plant density of 70 thousand plants ha-1-. The maximum yield, depending on the agrotechnical factors, was 10–12 t ha-1 in 2015, while in 2016 it was 14–16 t ha-1. Yield stability can be increased using hybrid-specific cultivation techniques. -
Grain Moisture of Maize Hybrids in Different Maturity Groups at Various Harvesting Dates
24-25Views:103The experiments were designed to determine the extent to which late harvesting helped to achieve low grain moisture content. The grain moisture contents of 24 hybrids from each of four different maturity groups were recorded during the last decade of September and the first decade of November over a period of three years (1999-2001).
The data indicated that late harvesting led to a substantially smaller difference between the hybrids. While in late September the difference between the grain moisture content of the earliest (FAO 200) and latest (FAO 500) hybrids was 8.9%, this value dropped to 1.5% over the average of three years when measurements were made in early November. With the exception of the earliest group, the grain moisture content in all the maturity groups declined during October. The later the hybrid, the greater the decline.
This change in the grain moisture content during October exhibited a considerable year effect. When the weather in October was warm, with little rain, the decrease was greater, while in cool, wet years the grain moisture content declined to a lesser extent, or in some cases even increased. -
On-line and off-line solutions in the precision weed control
249-252Views:128There are two directions of the development of the methods in precision weed controll. One is the on-line, either is the off-line solutions. The advantige off the on-line method is that it covers all the territory of the field, therefore there are no problems in sampilng methodology. At the same time picture processing is slow and the performance of the machines is low. We have choosen the off-line precision weed controll in Hungary to start with and we have solved the precision wedd controll of wheat, maize and sunflower successfully. We have also conducted on-line developments with the application of Weed Seeker sensor.
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Performance of agricultural factors on yield of sweet corn (Zea mays L. Saccharata ) - A review
143-156Views:150Sweet corn producers and industries require more reliable cultivars which could be accomplished by hybrid breeding. However, progressive phenological growth may be affected by different factors. In this paper, we analyze the key factors that determine the growth and yield of sweet corn. Environmental factors such as temperature and photoperiod were strong determinants of dates of flowering and harvest which are often crucial to yield in diverse climates and agricultural systems, besides the country's pedological conditions, especially soil fertility, affected phenological development. The effectiveness of fertilization in improving sweet corn growth performance was significantly influenced by the soil characteristics, the water supply, the genotype, and the agrotechnological factors. Therefore, genetic improvement of hybrids should be incorporated into the climate and soil elements to stabilize sweet corn yields in various agroecosystems. Decisions made in the sowing period are very significant, as up to 30% of the obtained yield may depend on making the proper choice. Deviation from the optimum date (either early or late sowing) may decrease yield. When deciding about the sowing date of maize, one needs to consider climate, soil quality, geographical location, temperature, weed infestation, sowing seed quality, and the ripening time of the hybrid to be produced.
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Evolution of some components of agroecosystems productivity from Vinga Plain in water stress situations
174-179Views:76The researches are inscribed on line of substantiation of durable agricultural system, having main objective the prominence of
quantitative and qualitative modifications made on agro-system level under the effect of no-tillage system for wheat, maize and soybeans.
The experimental field is placed on a cambium chernozem, with a medium content of clay, dominant in the Prodagro West Arad agrocentre
and representative for a large surface in the Banat-Crisana Plain.
The passing to no-till system change the structure of technological elements, through less soil works, so the impact on agro-system is
different comparing with conventional tillage, first less the intervention pressure on agro-system ant secondly appears new interactions, new
equilibriums and disequilibriums.
Considering the evolution of soil humidity, the observations made monthly (by taking soil samples and laboratory determinations) for
the three cultures showed that in the no-till system, there are more uniform values in the soil profile, and in the variants where the deep work
of soil was made it could be observed a low increase of the water volume in the soil. -
The Role of Trichoderma in the Rhizosphere of Tomato Plants
67-69Views:73It is well established that microorganisms are closely associated with the roots of plants can directly influence plant growth and development. Species of Trichoderma, on the other hand are primarily studied for their ability to control plant disease. The ability of species of Trichoderma to directly promote or inhibit plant growth has been noted for many years.
Tomatoes were treated with different Trichoderma strains by seed treatment and soil inoculation. The Trichoderma were grown on malt-agar medium and conidia were washed off by sterile water for making suspension which contained 107 CFU/ml (colony forming unit/ml). The suspension was used for seed treatment and for the soil inoculation by watering as well. The artificial soil inoculation was made by Trichodermas growing on grounded maize were mixed in mould and tomato seed were sown in it. Tomato seeds were also sown in bags made of close-meshed material which allowed the soil microorganisms to colonize the roots but it simultaneously protected the roots from soil contamination. Roots were put on Trichoderma selective medium to check the root colonization of the Trichoderma.
The tomato plants were bedded out in a field in four repetition. After harvesting by hand the results supported by statistics shown that there was significant differences between the yield of the untreated and treated tomato plants by Trichoderma strains. -
Evaluation of blue and red corn landraces
239-243Views:136Corn breeding can be successful only on a broad base of genetic material. The expansion of breeding aims includes the research of germplasm materials. In our experiments, we examined 11 blue, purple and red exotic corn varieties on two locations in Hungary. We conducted a complex study to obtain more information. We studied several morphological and phenological features and determined the most important qualitative parameters (protein, fat, ash). We applied the Tassel Area Index (TAI) under Hungarian conditions and compared it with the pollen producing ability of different genotypes. Results show that there are great opportunities in exotic corn varieties. Their quality exceeds that of the yellow ones in many cases. There are significant differences in yield and nutritional parameters. The favorable nutritional composition is not coupled with great productivity. Based on their flowering time and their agronomical features, they can be cultivated under Hungarian conditions as well. They match the new breeding aims, so they can be used as functional food or energy plants. With their high pollen producing ability and TAI, they can be utilized as markers in future pollen research.
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Effects of the cropyear and the agronomical factors on agronomical elements of different sweet corn (Zea Mays L. convar. saccharata Koern.) genotypes in long-term experiment
105-110Views:116In the crop season of 2010 (rainy year), we studied the effect of three agrotechnical factors (sowing time, fertilization, plant density) and four different genotypes on the agronomical characteristics of sweet corn on chernozem soil in the Hajdúság. The experiments were carried out at the Látókép Experimental Farm of the University of Debrecen. In the experiment, two sowing dates (27 April, 26 May), six fertilization levels (control, N30+PK, N60+PK, N90+PK, N120+PK, N150+PK) and four genotypes (Jumbo, Enterprise, Prelude, Box-R) were used at two plant densities (45 thousand plants ha-1, 65 thousand plants ha-1). The amount of precipitation in the season of 2010 was 184 mm higher, while the average temperature was 0.8 oC higher in the studied months than the average of 30 years. Weather was more favourable for sweet maize at the first sowing date, if we consider the yields, however, if we evaluate the agronomical data and yield elements (number of cobs, cob length and diameter, the number of kernel rows, the number of kernels per row) it can be stated that the size of the fertile cobs was greater at the second sowing date due to the lower number of cobs. The largest number of fertile cobs was harvested in the case of the hybrid Enterprise (72367.9 ha-1) in the higher plant density treatment (65 thousand ha-1) at the fertilization level of N120+PK when the first sowing date was applied. The largest cobs were harvested from the hybrid Box-R (cob weight with husks: 516.7 g, number of kernels in one row: 45.7) at the lower plant density (45 thousand plants ha-1) in the second sowing date treatment. Cob diameter and the number of kernel rows were the highest for the hybrid Prelude.
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Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)
93-96Views:172Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered next to Debrecen city (Hajdú-Bihar county, East Hungary).
In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.
The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.
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Analysis of the plant physiological effects of late, artificial corn smut infestation using remote sensing methods
31-35Views:177In Hungary, corn is also infected by several important pathogens. In this experiment, we analysed the plant physiological effects of artificial late cron smut infestation using remote sensing methods under field conditions We examined the experimental area from which the data comes from with a DJI Phantom 4 Multispectral Drone NDVI and NDRE indices were calculated and analyzed in GIS programs. Individuals treated with a higher dose remained much greener than the untreated control. They also showed significant differences within the indices used.
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Development of seed analyses by means of various matrix solutions and the MALDI-TOF MS technique
53-57Views:338The earth's population is growing steadily, currently accounting for about 7.3 billion people. Population growth causes food demand to rise, approximately 36 million people die each year due to starvation or related diseases. One solution to this problem is the continuous examination and development of the agricultural economy. In this study, matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI -TOF MS) were used to analyse of sunflower, soybean and hemp. In order to analyse the protein of maize, this method has already been applied. However, for sunflower, soy and hemp, it is necessary to develop a sample preparation method. Choosing the optimal matrix solution for ionization the traget molecule is an essential part of developing the method. Our aim is to compare two different matrix solutions (α-HCCA, SA matrix), based on the properties (intensity, noise ratio, value of spectra) of the spectra.