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Fusarium culmorum isolated from rhizosphere of wooly cupgrass (Eriochloa villosa) in Debrecen (East Hungary)
93-96Views:172Wooly cupgrass (Eriochloa villosa) is an East-Asian originated weed species and it has been spreaded worldwide by now. The first occurrence of this species in Hungary was observed and published in 2008 nearby Gesztely village (Borsod-Abaúj-Zemplén county, North-East Hungary) than in the summer of 2011 a significant population was discovered next to Debrecen city (Hajdú-Bihar county, East Hungary).
In 2013 this weed was also reported from Szentborbás village, Somogy county (South-West Hungary). These observations of spreading and its biological features (production of stolons and large number of seeds, moreover herbicide tolerance) indicate that wooly cupgrass (E. villosa) has a great potential of invasiveness, so it may become a hazardous weed not only in Hungary but in all over the world.
The objective of this study was to identify the fungus which was isolated from wooly cupgrass (E. villosa) root residue samples which were collected after maize harvesting on arable land in late autumn, near Debrecen. The identification of the fungus based on morphological characters of colonies and the features of conidia developed on potato dextrose agar (PDA) plates. After the examination of axenic culture we revealed that the fungus from rhizosphere of wooly cupgrass was Fusarium culmorum. Pathogenicity and/or endophytic relationship between the fungus and wooly cupgrass is still uncertain so pathogenicity tests and reisolations from plants are in progress.
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The Present and Future of Tokay Aszú
87-92Views:99Tokay wine is Hungary’s noblest wine. Beyond doubt, it is one of those uniquely Hungarian products, which has been without rival for centuries. Today the role of quality has become increasingly important and consumers who are ready to pay more for quality wines expect it. This wine will be able to get back its original popularity if the message of its excellence reaches the public. Knowledge of historical wine-producing regions constitutes a part of basic education and wine-marketing could take important measures to spread it since good products need to be sold properly and consumers require information about wines – with gastronomy – and wine producing regions.
With foreign invested capital, advantageous changes have been started in Tokay-Hegyalja. However, there is much still to do in order to produce, sell and consume Tokay wine on such conditions that are really worthy of a Hungaricum. It is essential to approach the famous oenologists of Europe and the world and to establish suitable production conditions for every winegrower. In addition to following consciously prepared wine-marketing directions, our country needs to make an expected name for this Hungaricum. These aims are important mainly because of approaching EU-accession. There is timely to study on what conditions Hungarian viticulture and oenology could access. It would be a great success of the Hungarian delegation if they could make current Hungarian laws of Tokay-wine-production accepted in the EU. Of course, all of these would be valid after EU-accession. During the pre-accession period, our country needs to fulfil several stipulations. For example, origin-protection, establishment of a registry system, law harmonisation, development of an institutional system.
Strengthening of home consumption has always been an inspirational tool in a product’s life cycle. It is important not only to take the requirements of foreigners into consideration and to produce for export, but we also need to satisfy the home market. Considerable stress should be laid on establishing and preserving viticulture. Increasingly, buyers expect high standards and they are ready to pay more for quality wines. They also demand appropriate professional information and consultation. So there is a lot to do and theoretical approach should be followed by action. In order to get to know what quality expectations exist, we have to examine the consumers of the Hungarian wine-market. Study of the Hungarian people’s consumption behaviour, referring to Tokay „aszú”, was closely connected with my work.
Participants of my study were consumers who buy Tokay „aszú” mainly in shopping centres, supermarkets and low-price shops specified for wine selling. Wine shops have important roles as they give opportunity for salespersons to make closer relationships with buyers. These shops could be communication channels of spreading wine culture. It would be favourable if in every town or city there were at least one wine shop giving the opportunity of consultation besides commercial activity and also providing services that support the improvement of wine culture’s position. The majority of questioned people spend less than 4.000 Forints on buying of Tokay „aszú” in a year. Results of the questionnaire survey support the fact that there is a demand for these wine-specialities, but lack of money strongly effects demand.
The Following summarises the history of Tokay wine, tasks of Hungary and the expected effects relating to EU-accession. Regulation of oenology and the wine-market, referring to quality wines and main results of the survey are also overviewed in the text. -
Setting up the operation of a Science Centre using process management tools
47-55Views:143Nowadays, Science Centres, which have a very long tradition in Europe, are continuously spreading in Hungary. These institutions primarily aim at raising public awareness of science in young people via informal learning (hands-on, minds-on etc.) methods. Based on this phenomenon, the construction of Scientific Adventure Park has been started in the Debrecen Botanical Garden. In addition, the Municipality of Debrecen established a new organization who will be responsible the reaching the defined goals and sustainability of Debrecen Science Centre according to the Application Form. Therefore, it will be designed a new institution with characteristics of Science Centre and provide innovative and complex services in the segments of culture and education. In this paper, I'm searching for answers to the question if based on this process-oriented method and management approach; it is possible to design operation system capable of achieving the strategic goals mentioned above.
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Effects of some herbicides on the microbiological characteristics of soil nitrogen cycle under maize plantation
93-100Views:89Nitrogen is a key element for the living organisms and influence not only for the quantity but for the quality of the yield, considerable. Availability of nitrogen from the soil is influenced by several microbiological processes of the Nitrogen-cycle. Among the intensive agricultural production the herbicide application cannot be omitted more information needs therefore about the inhibitor effect of herbicides on the different microorganisms.
An experiment was set up on calcareous chernozem soil under maize culture. Effect of four different herbicides (Acenit, Frontier, Merlin, and Wing) was investigated. The effect of herbicides was measured to four microbiological parameters of the Nitrogencycle (abundance of nitrifying bacteria, nitrate solubilisation, biomass nitrogen and urease enzyme activity). There were singledouble- and five times of recommended doses of herbicides applied for two onsecutive vegetation periods.
From the results of the different doses of herbicides, the following can be stated:
– The Acenit has a stimulating effect on nitrifying bacteria in general. The Frontier and Merlin also influenced the quantity of nitrifyers, however in certain cases decreased in another cases increased the number of bacteria.
– The double doses and five times doses of herbicides was found to be increasing the nitrate content of soil, -especially in 2006.
– The quantity of microbial biomass nitrogen increased in the 60% of treatments and decreased in the 40% of the treatments.
– Except of the result of Wing in 2006 and Merlin in 2005, the effect of simple dose herbicides was the smallest on the urease enzyme activity. According to the results the effect of Merlin was positive; the effect of Wing was negative on the soil enzyme’s activity.
Regarding the application of four different herbicides in three different doses on the microbiological parameters of soil (at two consecutive years-) in 39% of the treatments has resulted a significant inhibitory effect, 28% of the treatments, however have significant stimulating effect on the parameters measured. More than 50% of the inhibitory effect was measured in case of the Wing, at more than 50% of the Frontier the microbiological parameters have not changed. -
Tulip bulbs preparation by heat treatment for forced culture in green house
70-72Views:97Tulip can be grown in parks as ornamental plant and cut flowers, or possibly in protected areas for cut flowers during winter and early
spring.
An important condition for the successful cultivation of tulips is the quality of the bulbs that are going to be planted and their
biological preparation.
Under the quality aspect bulbs have to be uniform and large, preferably with circumference greater than 11 cm and must have good
phytosanitarical status.
Preparation of organic bulbs for ensuring the continuation of the differentiation process of germs (floral), is made during the storage.
It is known that the preparation of organic bulbs are made in interaction with environmental factors, among which temperature plays an
important role. At the beginning and throughout the process of biological preparation, temperature is maintained at a higher level then the
temperature must be low until planting.
The temperature level and the duration of treatment varies by followed objective respectively early or late flowering and staggered
flowers production over a longer period of time. -
A morphological survey of Ustilago trichophora, a smut fungi and evaluation as bioherbicidal agent for barnyard grass (Echinochloa crus-galli)
91-93Views:167Ustilago trichophora (Link) Kunze is a widespread smut fungus in all over the world. This fungus is pathogenic on species of Echinochloa genus. The subject of present research was that smut fungi occurrence on barnyard grass (Echinochloa crus-galli). Numerous barnyard grass plants with symptoms of smut galls caused by Ustilago trichophora on stems were collected from two counties, víz. Hajdú-Bihar (East-Hungary) and Jász-Nagykun-Szolnok (Middle-Hungary). The infested plants were examined in laboratory, which included the observation of the symptoms and the morphological properties of the spores and the colonies developed from sporidia. The results suggested that this fungus could be effective against barnyard grass weed so the biological control of this weed plant (Echinochloa cruss-galli) can be managed by using Ustilago trichophora biopreparates. As Ustilago trichophora can produce abundant sporidia in liquid culture, a high effectiveness control should be apply by Ustilago trichophora smut fungus as a mycoherbicide in Echinochloa weed control.
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Molecular Biological Approach of Crocus sativus L. and its Allies
112-115Views:168The hay saffron (Crocus sativus L.) is a sterile triploid plant, known in human culture only, with no fertile seeds produced. The origin of saffron is still a mist, however it is assumed to be an autopoliploid mutant or a hybrid. The recent classification and most of the former taxonomic publications define C. sativus to be derived from C. cartwrightianus, a wild species. Because of the sterility of hay saffron it seemed to be reasonable to apply molecular biological methods to complete classical taxonomic studies in examining its relations. The DNA polymorphism based AFLP method has confirmed the close relationship between these species.
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Comparison of the technological background of aquaponic systems
47-52Views:324Aquaponics is the combined culture of fish and plants in recirculating aquaculture systems, an ecologically sustainable horticultural production technique with long traditions.
The objective of this study is to compare flood-and- drain, and the water crossflow system and examine the differences in the water quality, fish yield and plant growth parameters for Common carp (Cyprinus carpio) and basil (Ocimum basilicum). During the study, water quality parameters of two treatments were compared in temperatures, pH, EC and NO3¯N were significantly different (p <0.05). Leaf area of the basil plants grew to an average of 20.37 cm2 (± 9.02 cm2). The plants’ biomass production was significantly different (p< 0.05) in the two systems. The biomass production showed lower yield, 458.22 g (± 214.59 g) in the constant flow system that in the flood- and- drain system 692.9 g (± 175.82 g). Fish Growth parameters were better in constant flow system (FCR 5.48 g/g ± 0.19). However, the specific growth rate (SGR) demonstrated that fish grew faster in flood- and- drain system 1.38 %/day (± 0.29).
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Settlement network, demographic circumstances, healthcare, social service and educations in Szabolcs-Szatmár-Bereg county
105-113Views:203Szabolcs-Szatmár-Bereg county shares its borders with three countries: Romania, Ukraine and Slovakia. The county is part of the North Great Plain Region, it is the third largest county of Hungary in terms of its population. The peripheric geographical location of the county gained importance by the EU accession, as the county represents a significant part of the eastern border of the EU.
Szabolcs-Szatmár-Bereg county is one of the counties of the Great Plain with significant population in the outlying areas, out of the six counties of the Great Plain the population of outlying areas is the lowest in the county, its ratio has been significantly reducing in the past decades. In the six counties in 1990 the population of the outlying areas represented 22.3% of the total population, whereas in Szabolcs-Szatmár-Bereg county the ratio was 13.6%. The ratio has dropped to 6.2% and 4.1% respectively.
The major aim of economic development is the improvement of the living conditions of the population, first and foremost by increasing family incomes. There are two main determining factors of family income level: employment ratio and the sectoral structure of employment.
Between 1992–2010 the number of employees in the financial sector decreased from 89 000 to 75 000, whereas the number of employees in the non-financial sector grew dynamically from 40 000 to 65 000. Structural change was significant. In the non-financial sector the proportion of the two main employment fields (education and healthcare) swapped. The number of employees in the education sector decreased from 16 000 to 14 000, while the number of employees in the health and social care sector grew from 11 000 to 22 000.
On the basis of statistics the number of kindergarten and primary school children reduced in the given period, while the number of secondary school aged children increased. In 1999 the number of secondary school children was 1/5th compared to the number of primary school children, whereas in 2010 their number almost reached 1/2. The student number in higher education doubled, which is a favorable phenomenon, though its structural changes raise some problems. The lack of technical trainings hinders exigent industrial development.
Since 1990 the education level of the population has significantly improved, the number and proportion of secondary school students and university graduates have risen much more dynamically than in other regions of the country. In the county the proportion of secondary school students increased 63% more than the national average, while in the case of university graduates the county passed the average national growth by more than 54%. Due to the effect of structural changes the proportion of university graduates in the corresponding age group improved, its backwardness compared to the national average has ameliorated from 34% to 29%, in the group of secondary school graduates the disadvantage has moderated from 26% to 21%. The computer and internet supply, as well as the number of teachers and students enrolled in secondary education institutions have developed dynamically in the county. Their growth has much excelled the national average.
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The effect of silver crucian carp (Carassius auratus gibelio Bloch) on the yields and the production value of common carp (Cyprius carpio) fingerlings
5-10Views:111During the last few years, the spread of the silver crucian carp has caused significant problems in pond fish culture. One of the main challenges of successful fish production in fishponds, is to rid ponds of the silver crucian carp. The nature of the damage caused by silver crucian carp is a complex question. To get into the ponds, the crucian carps occupy the territory before the common carp, and competes for food with common carp. The silver crucian carp has a less favorable food coefficient than the common carp, and it’s value is also lower. Therefore, the income of the fish farm decreases. In the course of the experiment, carp fry were stocked in 5 nursing ponds at the same stocking rate, with different (0,- 50-, 100-, 150-, 200%) stocking rates of silver crucian carp fry, respectively. At the end of the experiment, the yield/hectar and the production values were calculated. The experiment proved that the presence of the silver crucian carp in the fishponds causes lower yields and production values.
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Some strategic aspects of animal protein production
11-19Views:194The access to food shaped human societies and dietary models throughout the history of mankind. Animal protein consumption became a part of human culture. Data are presented showing the relationship of daily calorie and animal protein consumption as affected by capita GDP changes. Examples are presented how genetic improvement of animal and fodder plants influenced the resource efficiency and the overall environmental footprint per unit product. The two examples presented are: the dairy industry of the USA the 1944 and 2007 situation, and the Hungarian broiler chicken sector considering data relevant to 1930, 1960 and 2010. In both cases, dramatic improvements in resource efficiency could be demonstrated. The agricultural area required to animal feed production was reduced by more than 80% in both cases per unit product. Future possibilities are briefly discussed, referring to the still unutilized land reserves of the Globe, the new evolving technologies in progress inclusive the CASPR/Cas 9 genetic editing methods.
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From Lovrin to Szécsény – remembrance to Béla Lipthay (1892-1974)
21-32Views:91Béla Lipthay lepidopterologist, entomologist, museologist, agriculturist, hussar lieutenant, life-saving Roman Catholic, descendant of the historical family Lipthay de Kisfalud et Lubelle did a long way from his home village Lovrin to Szécsény, the one-time land of his ancestors. His life coincided with the disintegration of the historical Hungary, and the most serious trials of the Hungarian society, culture and spirit. These changes affected him as a member of Hungarian aristocracy many times and in fact wanted to destroy him. The fortune of the ancestors have been swept away by the storms of the wars, confiscated by the beneficiaries of tricky ideas of the new age and decimated by practical swindlers, but the human strength of character, the consciousness, the advantage of education and the with them associated ability, diligence, inventiveness and the sanctuary of faith have remained. All these made him possible to survive, to do his everyday hard creative work, which gained him the affection, the esteem and respect of the people living around him.
Protecting his remembrance we evoke his many-sided, altruistic efforts for the Hungarian people and Hungary, and we can contribute to the creation of a better and just world. -
Wine tourism of Tokaj- hegyalja
125-127Views:200The wine is still in Hungary in Hungarian history, culture, and one of the key elements of the economy are considered . Last, but not to the point of producing and selling the wine regions of livelihoods. Thousands of years in wine-producing traditions preserved in the modern winemakers, wine competitions demonstrate their skills. The Tokaj wine region of the World's major and significant wine regions in Hungary.
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Evaluation of decreasing moisture content of different maize genotypes
147-151Views:292An experiment was conducted to evaluate the decrease in grain moisture content in three maize hybrids in Debrecen in 2017. Armagnac, Sushi and Loupiac were the examined hybrids in this study. The culture medium and temperature conditions were applied uniformly for all three hybrids. According to the results obtained from the ratio of moisture content of seeds per day, the Armagnac variety in the intensive drying down phase loses more time and moisture content, so it can be concluded that the produced dry matter is more than in the case of other varieties. Armagnac requires more time to achieve yield, while the Sushi and Loupiac hybrids produce less harvest. Regarding the slope of the regression line, the rate of loss of moisture in the grain has been negatively correlated with the amount of “b” in the three examined hybrids. In regression analysis, the coefficient of explanation showed that the effect of day in the Armagnac was 97% in the Loupiac, 95% and in the Sushi 90% of the total dynamic value of moisture motion.
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Hungarian wine marketing ang wine consumption
43-47Views:156Hungarian wine is not in such a bad position as it is considered. We have outstanding specialists and the country’s natural conditions are also proper. The level of Hungarian wone culture is comparatively low but is capable of progress. Hungarian Wine Marketing Non-profit Company helps public wine marketing to be really effective. The development of tourism in wine-growing regions and advertising would considerably promote the popularity of Hungarian wine.
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Result of Wine Consumption Survey
387-393Views:123Wine has always played an important role in the Hungarian culture and gastronomy. Today, average wine consumption is about 33 liter per person per year, which figure, comparing with datas in Western Europe, is acceptable. However, cultured wine consumption has begun to develop only in last few years. Consumers, whose knowledges about wines have been widening, demand quality wines. The number of high standard scientific contributions on this subject has risen greatly. But comparisons of them are rather difficult because of adopting different surveying methods. This research has supported and confirmed that wine is a confidental product and previous experience could be an important determining factor during wine-purchasing. Wine is popular as a present, so the nice, elegant look, proper bottles and labels are essential for the higher rate of selling. Further, price-value rate could also be a main factor during the customers’ decision. This survey is a result of a test-research and its methodolical experience will have been used in my following researches.
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Rippl-Rónai’s color in the native parks: production and using of new, synthetic Wild sage color-mixture
59-64Views:194The climate changes is becoming more damaging to ornamental plants. Besides ornamental species and varieties of plants on public spaces. It will be necessary to use domestic, well adaptable climate-tolerant plant species.
One field of our growing ornamental plants researches from 2001 in the Centre of Agricultural Sciences of University of Debrecen, is studying drought patient, mainly Hungarian improved annual varieties, which are able to get acclimatized with the landscape. Moreover, we have in view to work out new, economical seedling production technologies. In the program, the excellent drought- and frost tolerant annuals and perennials belongs to the climate change tolerant plants. From the evaluated of species we want to create and spread the application. of the new types of ornamental horticulture culture.
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Relationships of the sport and the life quality in the University of Debrecen
87-96Views:175In the sport- life of the University of Debrecen there has been a demand formulated on the making of a comprehensive, long-term development plan, which obviously incorporates all
fields of sport. This document guides the way for people in the sport- life of the university how they could organise their activities connected with sport on a calculable, predictable level.
Necessarily, the preliminary collection of professional informations has been a basic requirement for the establishment of this document.
The program provides foreseeable instructions to the organisation of sport pursuits both for sport people in the university, and members of the sport spehere in a wider sense. It
offers a cohesive solution for all kinds of tasks defined with the sport life, and also gives a guidance for the various ways of realisations of these challenges. Thus it can answer such questions as for example how the university can conduce the initation of conditions for the healthy living through spare time sporting, how it can participate in the financing of contest sport with the contributing project of reinforcement training and how the university can support the sport life of handicapped people. The project also contributes to the cultivation of the sport traditions of the university by bankrolling not only the official university sport
life but also the leisure time sporting of the students.
On the basis of rethinking the situation of sport at the university with its future possibilities the program paraphrases the tasks of the university in the sport life, the long term aims of
university sport politics with its operative aims that should be performed and also the possible steps which lead to these purposes.
The target of the project is to turn the University of Debrcen in every field into a “Sporting University”. It means, that beyond the development of quality sport and outstanding contest sport a much wider range of university people should be included in sporting in order to utilize all the advantages of sport. With this the physical culture could become not only a substantial form of spending our free time, but with getting one of our natural everyday activities it can also become our lifestyle.
Sporting prominently contributes to both the university students’s and the whole society’s mental and physical wellfare, and it also helps to improve our helthstate on various fields of life:
with its special functions it is a means for establishing a healthy nation, for promoting common relationships, for nourishing the economic activity of people and also for strengthening the national values and one’s self-esteem through providing equal opportunities.
The prosperousity of contest sport at the university and the maintaining and protesing of its efficiency fortifies our selfregard, as it is able to hold appropriate sportsmen up as examples
to both present and future universtity students, and also offers exacting ways of entertainment. Besides all these the advataged purpose of the project is to find and delibaretaly include those
necessary mechanisms which are needed for the working of the sport market. -
Possibilities of Growing Artichoke (Cynara scolymus L.) in Hungary
126-130Views:103The domestication of choice broadening, special vegetable species offers new market possibilities in Hungary. The production of artichokes has good perspectives due to its high protein, vitamin and mineral contents and its wide usability. According to the literature, in Hungary it is worth examining the annual production technology, which has yet to be researched in our country. The basic aim of our work was to establish the annual production of artichoke in Hungary with scientific research.
In the experiment, 5 Italian sorts (Valetta di Roma, Gobbo di Nizza, Violetto di Romagna, Violetto S. Francesino and Precoce di Romagna), 1 American sort (Green Globe) and 1 Egyptian sort (Balady) were used. For one half of the plants, we tried to bring on bud-production appearing in the first year only with early planting, while we used also a gibberellic acid hormone treatment for the other half.
Only the sort Green Globe had yields in appropriate quantity (21.4 t/ha) and quality. The low yield and inequable quality of buds of the Italian and Egyptian sorts refers to the fact that these sorts are not applicable for annual production in Hungary. Although the gibberellic acid treatment resulted an earlier harvest, increased the equality of buds and increased the proportion of stems producing buds except for the sort Green Globe, overall it did not have a positive effect. The hormone treatment fastened the physiological processes of the plants, thereby it induced early insenescence and lignifying of the buds. The high temperatures during the bud-formation period largely increased the ratio of flabby, over-blowing buds, so the planting must be timed in the future that the harvest period is in early automn or late spring, early summer months.
According to our results, globe artichoke can become a perspective culture by applying cold-resistant sorts with short growing seasons, which can be produced as annual crops without hormone treatment by the appropriate timing of bud-formation. -
Addictions of youngsters living in the countryside: social and demographic background of alcohol consumption
81-84Views:140In the past 10–15 years, the consumption of illegal drugs has increased significantly both in Hungary and in our county. In parallel with the society’s getting more and more open, the fulfilment of personal freedom, and the spreading of globalisation and individual culture, the use of drugs has become more and more accessible and acceptable to the young.
Social problems are supposedly connected with the interviewees’ alcohol consumption. The EuroADAD questionnaire was used. The sample covered 190 interviewees. I have processed the data with the SPSS statistical program package.
The linear regression analysis was applied to examine the interrelations between the elements under study. Almost consume alcohol as well. Among the examined socio-demographic variables, youth alcohol consumption was most affected by the gender of the respondent, the dissatisfaction with living conditions, and the change of residence.
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Evaluation of nutrient conditions in open hydroponic system based on tomato production
116-119Views:84Monoculture caused a gradual decline of soil conditions, while nematodes and salt accumulation stimulated the growers to choose alternative practices, such as soilless cultures, which proved their value in Western Europe. Exact statistics are lacking, but estimates deal with approximately 300-400 hectares of vegetable on rock wool, whereas other substrates of soilless culture may multiply this number. Real perspectives are attributed to the forced production of pepper, tomato and cucumber.
Vegetable production in greenhouses may impair the ecological balance of the environment substantially as far as being uncontrolled. Soilless cultures especially should be handled thoughtfully. A fraction of the nutrients administered, more than 25-30%, is doomed to be lost in an open system, and the resulting ecological risk is accompanied with increasing costs of the production.
In Hungary, the quantity of nutrient elements in drainage water is unknown, et all. Connecting the production results with chemical analysis, we gain more information about it.
You can see a mathematical method for evaluation of nutrient and water conditions in tomato hydroponics production. -
Improvement of black locust (Robinia pseudoacacia L.) growing under marginal site conditions in Hungary: case studies
129-133Views:362In Hungary, black locust (Robinia pseudoacacia L.) is considered as an important exotic stand-forming tree species growing mostly under unfavourable ecological conditions for forest management. Due to climate change effects, its importance is increasing in many other countries, too. As a result of a selection programme, new black locust clones were tested in clone trials. Juvenile growth of 12 micropropagated black locust clones in two plots series established at different dates were evaluated in central Hungary under marginal site conditions. At age of 7 the clone R. p. ‘Bácska’ (‘KH 56A2/5’), at age of 10 the clones R.p. ‘Homoki’ (‘MB17D3/4’) and ‘PV201E2/4’ appeared to be especially promising for mass production. Based on the data obtained from the performed trials, it can also be concluded that tissue culture can be considered as a suitable tool for propagating superior individuals and offers new prospects for the rapid cloning of selected genotypes used for plantation forestry.
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Identification and biological examination of some inland Monilinia species
27-29Views:153The aim of this study was to identify and biologically analyse some Monilinia species from Hungary. 146 M. fructigena and 28 M. laxa species out 174 infectious fruit from all over the country were used for the study. For further study 10 isolates were used and apple fruit was inoculated with them according to Koch postulate. 1–2 mm ochre exogen stromas were observbed on infectious plant parts and growing signs on culture of all isolates were identical to M. fructigena. To affirm classical identification, isolates with molecular biological method were also prepared using PCR reaction. Control isolates of M. laxa, M. fructigena and M. fructicola were used. The size of PCR product showed that all isolates had a 415 bp band which was typical of M. fructigena. Results support the previous observation that M. fructigena and M. laxa species occure all over Hungary.
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Phylogenetic studies of soybean pathogen Phoma species by Bayesian analysis
53-61Views:193We carried out phylogenetic study analyzing sequences of genetic markers in the taxonomy of Phoma and Phoma-like fungi. Different species of Phoma and Phoma-like fungi occurring on soybean (Phoma pinodella, Phoma sojicola, Phyllosticta sojicola, Phoma exigua var. exigua) are difficult to identy because of their high morphological and symptomatic similarities.
Twenty-two isolates of nine different Phoma species were obtained from reference culture collections. Seven of them were isolated from soybean, the others were collected from different hosts.
The Phoma isolates were firstly characterised by morphologically, and then we employed a part of the gene responsible for the synthesis of translation elongation factor 1 subunit alpha protein (tef1), ITS region, as well as β-tubulin partial sequences as potential genetic markers to infer
phylogenetic relationships among different Phoma species..Finally, their ITS and tef1 sequences were sequenced and analysed by Bayesian approaches.
According to phylogenetic trees inferred by Bayesian analysis of tef1, ITS and β-tubulin sequences, different Phoma species can be separated proving that these phylogenetic markers are well suited for phylogenetic studies of Phoma species. However, the phylogenetic tree does not support the traditional Phoma sections based on morphological characterization.
Bayesian analyses of the three sequences confirmed that the Phyllosticta sojicola species is clustered with the Phoma exigua var. exigua group and the Phoma sojicola is grouped with Phoma pinodella group. The molecular data provide evidence for reclassification of formerly mentioned soybean pathogens. -
Experiments for Isolating and Culturing Soil-borne Mycobacteria (Contemporary Publication)
107-110Views:71On grounds of the several thousand tests performed in the field of this topic, the following conclusions may be arrived at:
1. The informations available and the experimental data on soil mycobacteria are very incomplete.
2. Of the 77 strains isolated from similar soil types so far, and adaptable for pure basic culture, 47 strains are confusingly similar, from morphological aspects, to the mycobacteria isolated from clinical material.
3. The apparently homogeneous cultures isolated from the soil are generally co-infected and, therefore, the morphological, biochemical, and other physiological characteristics of the isolated strains can be studied only on base cultures after purification.
4. For the isolation of the soil mycobacteria experiments qualified hitherto as most suitable processes the 4 or 1 per cent NaOH neutralized with H2SO4, and the 1 per cent NaOH or 1 per cent Na3PO4 treatments, on Gottsacker agar medium with plate or top pouring, at a temperature of 29 to 37 C°, in a soil suspension sequence of 1:500 to 1:5000 final dilution.
5. The Ziehl-Nielsen staining of the isolated mycobacteria composed to sub-cultures is best performed by heating with an infra red radiator from above, instead of the gas flame used so far to heat from below.
The repetition of the biochemical test of the hitherto isolated 77 purified strains is under progress, and will be reported on in our next scientific publication.