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  • Agricultural Biotechnology MSc. course and agriculture in the 21st century
    208-214
    Views:
    93

    The establishment of an Agricultural Biotechnology MSc. course creates a new direction in Hungarian higher education. As an introduction, the article summarizes the main theoretical and practical possibilities and results of biotechnology which have necessitated launching the course. Subsequently, the preliminaries, aims and requirements of the Masters course are introduced. The main data of the agricultural MSc course at Szent István University in Gödöllő (Hungary) are the following: 4 semesters, 120 credits, 1200 contact hours, 4 weeks training in a biotech laboratory, 6 fundamental subjects, 8 basic subjects in biotechnology. Plant and animal biotechnology, are the two options for specialization and both have 10 separate professional subjects. The teaching staff consists of 34 teachers (93% of them have PhDs or higher scientific degrees) from 10 Departments of 3 Faculties of Szent István University.

  • Effect of different weaning time on the growth and survival ofCommon carp (Cyprinus carpio L.) larvae
    55-59
    Views:
    390

    Within our experiment, we studied the combined effect of live food application and feed training on the production parameters of carp larvae. In addition, we examined whether there is a difference in the growth rate and survival rate of the specimens of Szeged mirror landrace reared in similar stocking density but with different treatments.

    Specimens of Szeged mirror landrace from induced propagation were placed into 12 pieces of 40-liter aquaria. For the 4 different treatments, (K, A3, A6, A9), live food (Artemia salina) was fed, as well as feed training was applied.

    We also sought to find an answer as to how our feeding and rearing conditions can affect the production parameters of common carp fries and what impact these conditions may have on their survival rates. At the end of the 2-week experiment we determined the individual weight gain of the carp fries, their survival rate and the biomass growth. The purpose of the study is to examine the optimum timing of shifting from live food to feed for carp larvae.

  • Recovery and confirmation of Haemonchus contortus from abomasal contents of roe deer (Capreolus capreolus) in Eastern-Hungary (Biharugra): A diagnostic case study
    59-62
    Views:
    168

    Gastrointestinal parasites are ubiquitous. They occur both in wild and domesticated animals. Among such parasites of veterinary importance is the trichostrongyle worms, out of which the Haemonchus contortus species is regarded as the most pathogenic one in the small ruminant industry. The occurrence of this parasite in the sheep flock is now very well documented and an established fact in Europe, although the parasite was original of the warmer climatic region. Studies on the cross-transmission of H. contortus between the wild and domesticated animals are also on the rise although the question of the direction of transmission is still debated. This is an important area that needs to be addressed as it could potentially contribute indirectly to mitigating anthelmintic resistance. Hungary also has reported its share of the occurrence of the parasite, mainly in the sheep flock and a certain population of roe deer. The study presented here is the preliminary results of a diagnostic case study that confirms the presence of H. contortus in wild ruminant deer species that are close to the domesticated sheep population.

  • The father of the term ‘biotechnology’ was Hungarian: The forgotten works of Károly Ereky
    107-110
    Views:
    374

    In the Nature, Robert Bud gave an account of the fact that the generator of the concept "biotechnology" was the Hungarian Karl Ereky who, in his book published in Berlin in 1919 entitled "Biotechnologie der Fleisch-, Fett- und Milcherzeugung im landwirtschaftlichen Grossbetriebe" ("Biotechnology of Meat, Fat and Milk Production in a Large-Scale Agricultural Farm"), disclosed his observations and new views in that regard. Later on, Ereky's two essays, published in German language again, and other contemporary German sources have been processed which have confirmed the assumption whereas – in scientific terms – the further first applications of the expression "biotechnology" can be attributed to Ereky's works. Recently, we have explored and found biographical sources and documents which had been published by Ereky, which, however, have already been forgotten, by dint of which the story of origin of the first concept "biotechnology" can as well be clarified in a factual manner.

  • Influence of 17-alpha methyl testosterone on the production parameters of common carp (Cyprinus carpio L.) fry
    37-43
    Views:
    270

    During our research we aimed at finding an answer as to what extent the different concentrations of 17-alpha methyl testosterone incorporated in the diet of common carp fries can influence the production parameters of the species, as well as how efficient their sexreversal can be with the use of this method. To this end, an aquarium experiment was conducted in the course of which four different hormone treatments were set and monitored. The fish feed was enriched with 17-alpha methyl testosterone in 50 ppm, 75 ppm, 100 ppm, 500 ppm dosages.

    The obtained figures revealed that the hormone treatments had no influence on the production parameters and conservation of the common carp fries. Further on, our team is to determine the sex of the fish through the examination of gonads during autopsy when they reach the 500 g average weight.

    Furthermore, a male specific test method which was supposed to be of great help in our attempt to select the sex-reversed specimens in the subsequent processes was also put to the trial. During the experiment the DNA-isolation of different sample types (muscle tissue, fin, mucus) of common carp with identified sex was successfully carried out. The extracted PCR product was examined with agarose gel. Our results indicated that the ccmf2 marker was applicable, however, the obtained figures were not reliable.

  • Deproteinized plant juice as part of circular economy: A short review and brief experimental data
    23-26
    Views:
    1538

    As the population of the Earth is constantly growing it generates an unmet demand for protein, which is an urgent problem. The protein extraction process is a potential solution, which offers high-quality plant protein suitable for animal and human nutrition at a favorable price. The process used within our project produces green juice from the green alfalfa biomass through pressing. After the coagulation of protein from this green juice, the by-product is called DPJ (Deproteinized Plant Juices) or brown juice. Our preliminary results match the international literature, namely that brown juice take up as much as 50% of the fresh biomass in weight. To utilize this by-product is a crucial part of the process to make it environmental-friendly and financially viable as well. The examined brown juice samples came from a small-scale experiment of alfalfa varieties carried out in the experimental farm at the University of Debrecen. According to our preliminary results, brown juice has high macro- and micronutrient values, furthermore, it has a potentially high amount of antioxidant compounds. The study highlights that brown juice is suitable as an ingredient in microbiological media, in plant nutrition as a supplementary solution, for feedstock and for preparing human food supplements or functional foods. The potential utilization of all biorefinery products makes it a very appropriate technology for today’s challenges.

  • The effect of dissolved oxygen on common carp (Cyprinus carpio) and basil (Ocimum basilicum) in the aquaponics system
    89-96
    Views:
    419

    Aquaponics is an integrated system that combines fish farming (aquaculture) and hydroponic plant production. The objective of this study was to examine how the level of dissolved oxygen with or without an air pump affects water quality, fish output and plant growth parameters for common carp (Cyprinus carpio) and basil (Ocimum basilicum).

    Ebb – and flood aquaponics systems (with automatic syphon) was used. Two treatments were set in this experiment, one of which was the aquaponics system without air pump (unit I), where water of the plant bed was pumped two directions, one falling back to the fish tank oxygenating the water the other was pumped to the hydroponics unit. The other system (unit II) was designed with an air pump.

    In the course of the study, water quality parameters, such as oxygen saturation, dissolved oxygen (DO), electrical conductivity (EC) and nitrite were significantly different (p<0.05). Total basil biomass was higher in unit II. (5367.41 g). The final biomass of common carp were
    2829.45 g ± 79.24 and 2980.6 g ± 64.13 g in unit I and unit II respectively. Weight gain (WG) and specific growth rate (SGR) showed no significant differences (p>0.05) between the treatments. 

  • Individual cage housing affects feed intake and induces sex-specific effects on body weight in Japanese quails
    137-142
    Views:
    173

    Individual cage housing in poultry production could be a potentially stressful environment for the birds that can alter feed intake, consequently induce negative effects on performance. Previous studies used individual bird-based experiments to extract the detailed molecular, physiological, and fitness outcomes of treatments. Understanding sex-specific effects of isolation on social birds such as Japanese quails (Coturnix japonica) may reveal important considerations. To understand this phenomenon, birds were kept in groups of 10 for one week and they were kept in individual cages for another week.  Daily feed intake (DFI) was measured each day and body weight was measured at the beginning, middle, and end of group rearing and at the beginning and end of individual rearing. It was found that both males and females showed a reduction DFI in response to individual rearing (p<0.0001). Females consumed on average 29.38% higher amount of feed than males. Additionally, females showed a pronounced reduction in body weight after isolation, while the effect on males was not significant. Similarly, females had on average 17.61% higher body weight than males. The body weight to DFI ratio was higher in males than females. The finding of this research revealed an important implication of isolation and sex differences.

  • Comparison of the technological background of aquaponic systems
    47-52
    Views:
    325

    Aquaponics is the combined culture of fish and plants in recirculating aquaculture systems, an ecologically sustainable horticultural production technique with long traditions.

    The objective of this study is to compare flood-and- drain, and the water crossflow system and examine the differences in the water quality, fish yield and plant growth parameters for Common carp (Cyprinus carpio) and basil (Ocimum basilicum). During the study, water quality parameters of two treatments were compared in temperatures, pH, EC and NON were significantly different (p <0.05). Leaf area of the basil plants grew to an average of 20.37 cm2 (± 9.02 cm2). The plants’ biomass production was significantly different (p< 0.05) in the two systems. The biomass production showed lower yield, 458.22 g (± 214.59 g) in the constant flow system that in the flood- and- drain system 692.9 g (± 175.82 g). Fish Growth parameters were better in constant flow system (FCR 5.48 g/g ± 0.19). However, the specific growth rate (SGR) demonstrated that fish grew faster in flood- and- drain system 1.38 %/day (± 0.29).

  • Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
    21-25
    Views:
    308

    Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.

    The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.

    After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.

    In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.

  • Genetic diversity of the Hungarian draft horse assessed by mitochondrial DNA
    29-32
    Views:
    254

    Hungarian draft is a horse breed with a recent mixed ancestry. It was developed in the 1920s by crossing local mares with draught horses imported from France and Belgium. To genetically characterize the breed and to set up the basis for a conservation programme, we have employed a molecular marker: a 256-bp D-loop mitochondrial DNA fragment. We analyzed 124 horses representing Hungarian draft horses to assess the maternal phylogeography of the breed. Sequence analysis of a 256-bp segment revealed a total of 34 haplotypes with thirty-four polymorphic sites. High haplotype and nucleotide diversity values (Hd=0.953±0.001; π=0.024±0.001) were detected. The average number of pairwise differences were k=5.998. This breed counts 800 mares today, and only survive due to breeding programmes, this way each haplotype frequency depends on the extent to which mares are involved into the breeding. The reduced number of surviving maternal lineages emphasizes the importance of establishing a conservation plan for this endangered breed. Due to the revealed 34 polymorphic sites we could presuppose twelve maternal linages, which could be a first step for making a breeding programme.

  • Seasonal variations in somereproductive parameters of Dorper Rams in Hungary
    17-20
    Views:
    307

    The purpose of the present study was to test the hypothesis that season has an affect on semen quality and scrotal circumference of Dorper rams. The experiment was carried out with six Dorper rams aged between 15 and 18 month. Semen samples were collected with artificial vagina and volume, concentration (x 109/ml), total sperm number/ejaculate (x 109), mass motility (0–5), progressive motility (%), scrotal circumference (cm) was observed. Significant differences (P<0.05) were observed in concentration, total sperm number/ejaculate, scrotal circumference in different seasons. Volume was the highest in autumn (1.4±0.5 ml) and the lowest in the spring (1.3±0.4 ml). Concentration of semen was lower in spring (2.6±1.5 x 109) and summer (3.3±1.5 x 109) as compared to fall (4.1±1.1 x 109) (P<0.05). Regarding total sperm number/ejaculate (x 109), scrotal circumference (cm) all the seasons differed significantly (P<0.05), although the season had no effect on mass motility and progressive individual motility (P<0.05). In conclusion the present study showed that semen quality parameters and scrotal circumference of Dorper rams were better in autumn than in the other sea- sons.

  • Effects of bioactive plant extracts on immune-related gene expression of common carp (Cyprinus carpio)
    49-56
    Views:
    311

     In recent years, intensive fish farming has led to an outbreak of several diseases, and the health status of fish can affect the economy of aquaculture. Since fish health and intestinal health are in correlation, it may also have an impact on immunity. Accordingly, many natural feed additives are being used to improve immune functions. In our study, carotenoids, oligosaccharides, and anthocyanins were applied at 1 m/m% in feed to investigate their effects on cytokines, such as interleukin-1β (IL-1β), interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), and interferon regulatory factor-1 (IRF-1) in spleen and mid-intestine of 6 months old carp. Gene expression analysis was carried out to examine IL-1β, IL-8, TNF-α, and IRF-1 mRNA levels in fish spleen and mid-intestine. The gene expression level of pro-inflammatory IL-1β decreased in the mid-intestine of carotenoid-fed carp compared to anthocyanin supplemented group, but the effects of the bioactive plant extracts were not observed on the examined cytokines compared to control fish.

  • SSR based characterization of peach (Prunus persica L.) and apricot (Prunus armeniaca L.) varieties cultivated in Hungary
    17-24
    Views:
    306
    The SSR (Simple Sequence Repeat) markers allow the discrimination of the cultivars and determination its specific DNA fingerprints. The aim of this research was to evaluate fifteen apricot (Prunus armeniaca L.) and fifty-one peach (Prunus persica L.) genotypes cultivated in Hungary to obtain their DNA fingerprints in 6 SSR (Simple Sequence Repeats) loci by allele numbers and sizes.
    DNAs were extracted from leaves. PCR was carried out with CY-5 fluorescent labeled Prunus microsatellite markers and the products were separated on polyacrylamide gel with ALF (Automated Laser Flourometer)-Express II.
    According to our results, in the case of peach genotypes, all 6 SSRs were able to amplify alleles. UDP 96 005 was the most informative marker and UCDCH 17 was the least due to its monomorphic pattern. Regarding the apricot samples BPPCT 041 did not amplify any allele. In the case of P. armeniaca UDP 96 005 had the highest heterozygosity index as well and the highest number of alleles. The least informative marker was the UCDCH 17. Since the 6 SSR were not enough to discriminate the apricot and peach genotypes, it is suggested to use more SSR primers.
  • Reference genes for livestock gene expression profiling – Literature review
    81-89
    Views:
    195

    Quantitative real-time polymerase chain reaction (qPCR) is an essential tool for understanding animal cell’s response to developmental progression or to different experimental conditions at gene expression level. However the reliability of this method heavily lies on proper normalization (measuring a target and a reference gene’s expression from the same sample to correct for technical related variations).
    Our literature review aimed to summarize the articles addressing the most important livestock species in regards of reference gene stability used as normalizers for quantitative real-time polymerase chain reaction experiments. Stably expressing reference genes were categorized into 14 distinct groups according to gene function. The number of reference genes tested and the publication numbers according to years and the ranking algorithms were also noted.
    Counting showed that genes encoding ribosomal protein components are ranked as most stable in majority of cases and therefore should be taking into account for qPCR stable normalizer gene finding experiments.

  • Routine microscopy examination of faecal samples as a tool for detection of common gastrointestinal parasites: a preliminary report from two Hungarian farms
    63-66
    Views:
    158

    Gastrointestinal parasitism in ruminant animals is a cause of major economic loss incurred by the livestock industry. Regardless of the frequency of the adopted therapeutic and prophylactic deworming strategies, the parasitic burden in a farm should be assessed regularly. One of the most widely used techniques to do so is the microscopic faecal egg examination and faecal egg counting method. Despite the technique being almost a century old from its first adoption, the principle behind the newer techniques of faecal egg examination is the same. This technique is still being used in routine farm screening and monitoring gastrointestinal parasitic load and faecal egg count reduction testing to assess the anthelmintic efficacy of the drugs used. Thus, the tool remains a choice for preliminary screening for important parasites and the subsequent deworming strategy. Our study here was part of a larger survey on the treatment efficiency as well as a broad epidemiological study of the trichostrongyle parasites in Hungary. We present a preliminary report on the detection of common gastrointestinal parasites from two farms in Hungary, including a species-specific confirmatory microscopy for Haemonchus contortus eggs.

  • Biotechnological methods for improving reproduction on sheep breeding program using laparoscopic artificial inseminations in Debrecen, Hungary
    193-200
    Views:
    117

    The aim of this study was to evaluate the results of the LAI method in different types of breeds and seasonal variations. Analysing n=536 LAI in 7 years, there were no significant differences observed in the breed in lambing rates. Although the lambing rate of Prolific Merino (F+) was better than any other breed (45.3%). We found significant differences between breeds of Tzigaia, its pregnancy rate was 19.2%. In the case of prolificacy there were significant differences between breeds: Prolific Merino’s (FF) was the highest (2.38) and, Indigenous Tzigaia’s was the lowest (1.4) from all of them. The obtained results showed that summer inseminations were the most effective in concern of pregnancy rate (49.5%). The results are based on use of the types of semen, showed that using frozen semen was most effective than fresh semen in both parameters, however there were no significant differences. Summarising all tested conditions in our study breeds, seasons and type of semen using LAI the total results of pregnancy rate was 40.3% and the prolificacy was 1.78, respectively. Follow up the out-of-season ovarian, progesterone level of n=36 Prolific Merino were examined and insulin, IGF-1, leptin, T3, and T4 hormones as well, to evaluate the energetic status of the flock. Progesterone analysis showed 43% of examined ewes had a cyclical ovarian function in April and a cyclical ones had good reaction for estrus-induction hormonal treatment. Our results showed that valuable information can be obtained about energy supply from the determination of some metabolic hormones as IGF-1, insulin, leptin.

  • Ragweed components in honey
    65-68
    Views:
    189

    The aim of this research was to prove that the syrup containing ragweed used for feeding the bees was transferred to the honey. To reach this goal we developed a method to analyse the volatile components of the samples. We applied two sample preparation methods. The first one was SPME (Solid Phase Micro Extraction) preparation from the headspace of all of the samples. In the second case only the raw leaves of the ragweed were extracted with n-hexane and dichloromethane to complete the SPME results. The measurements were performed on GC-MS equipment. Chromatograms and data evolution showed that the components of the ragweed were also present in honey so they were transferred by bees.

  • Impacts of ethnoveterinary medicine integrated with livestock farm technologies on the performance and antimicrobial resistance of indigenous goats in South Africa –A review
    93-103
    Views:
    124

    Ethnoveterinary medicine and livestock farm technologies represent a junction of innovation and tradition with the aim to accomplish livestock systems that are resilient, productive, and sustainable to meet 21st century challenges, especially the antimicrobial resistance issue. This article aims to emphasise the impacts of integrating ethnoveterinary medicine with livestock farm technologies on the performance of indigenous goats in South Africa. With the growing concern over antimicrobial resistance in livestock, there is a pressing need for complementary approaches to the health of animals and their productivity. In this review, the aim evaluated whether an integrative approach could offer a sustainable alternative that benefits animal health and productivity while addressing the concerns of antimicrobial resistance. Comparative study designs across multiple indigenous goat farms were used to integrate specific medicinal plants in ethnoveterinary medicine with present-day livestock technologies that are used to monitor the effects on livestock performance indicators and antimicrobial resistance patterns. In the treated populations, the outcomes were indicative of substantial improvements in reproduction and growth rates, and alongside, there has been a notable decrease in the markers of antimicrobial resistance. Therefore, it is proposed that through these discoveries, the integration of these traditional and modern approaches not only improve the performance of indigenous goats but also contribute immensely to the mitigation of risks associated with antimicrobial resistance.

  • The significance of local meteorological stations in research planning
    87-91
    Views:
    235

    The goal of research planning is to optimize human and material resources while maximizing efficiency. If there are databases that can be used as a substitute for own data collection, effective research can be facilitated by reallocating resources. In the case of environmental studies, the knowledge of the climatic conditions of the study period is known to be a key factor in research planning process.

     In the present study, the data of our meteorological station in our research area (known as “Szamárháti Tanya”, Kesznyéten, Hungary) was compared with the measurements of meteorological stations operated by the competent water authority. Stations were taken into the study within a 10 and 20 km radius over a period of 21 months, to determine which provided more relevant data from the area.

    During the evaluation of results, the relationship between the local and regional weather stations were determined, and deductions were made from the obtained results to support the decision which of the targeted investigations could be of greater benefit. 

  • Some population genetics parameters of the present Hungarian Hucul Horse population
    15-22
    Views:
    183

    We examined the Hungarian population of the Hucul horse breed, under genetic protection, based on population genetic indicators until the year 2014 included. The evaluation was performed using the Endog programme based on the following indicators: inbreeding coefficient, average relatedness, the maximum number of generations, the number of full generations traced and offspring number. Our findings were as follows: the average inbreeding coefficient of the total population was 5.99%, average relatedness was 11.82%, the maximum number of generations was, on the average, 16.04%, and the number of full generations traced with reference to the whole population was 4.15% on the average. 40% of the whole population (723 individuals) did not have any offspring; 42% (759 individuals) attained an offspring of 1 or 2, while 3.4% (88 individuals) had a surviving offspring of 3. The highest offspring number according to the national database (92) was attained by one stallion.

  • The effect of β-glucan, carotenoids, oligosaccharides and anthocyanins on bacteria groups of excreta in broiler chickens
    15-20
    Views:
    258

    This study was conducted to examine the effect of natural compounds, such as β-glucan, carotenoids, oligosaccharides, and anthocyanins in the diet on bacteria gropus of excreta in Ross 308 broiler chickens. Chickens were fed 5 diets: control (basal) diet, a diet supplemented by β-glucan at 0.05%, and diets supplemented by carotenoids, oligosaccharides, or anthocyanins at 0.5% of each compound. On experimental day 19, excreta were collected to determine the proportion of Lactobacillus, Bifidobacterium, Campylobacter, Clostridium, and Escherichia coli. Samples were collected aseptically and snap-frozen in liquid nitrogen. Bacterial DNA was isolated from samples, then polymerase chain reaction using primer pairs designed to the 16S rDNA of bacterial groups were applied to define the proportion of the mentioned bacteria. Another universal primer pair was used to amplify a region of 16S rDNA of all the examined bacteria. Proportion of each bacterial groups was determined relatively to the intensity of universal PCR product band by gel documenting system and ImageLab software. Based on the results, carotenoids and anthocyanins increased the proportion of Bifidobacterium, which might imply the beneficial effects of the mentioned compounds on the bacteria composition of excreta.

  • Identification of the Slovak traditional cheese “Parenica” microflora
    227-239
    Views:
    266

    Numerous studies have demonstrated the higher accuracy, faster time-to-results and lower costs provided by MALDI Biotyper systems compared to classical methods. In this study, the culturable population of total count of bacteria, enterococci, coliforms bacteria, lactic acid bacteria (LAB) and microscopic fungi and yeasts from cow’s dairy products was identified using the MALDI-TOF MS Biotyper. Altogether, 50 samples of the Slovak cheese “Parenica” were examined. Total numbers of bacteria were cultured on Plate count agar at 37 °C for 24–48 h, aerobically; enterococci were cultured on Enterococcus selective agar at 37 °C for 24–48 h, aerobically; coliforms bacteria were cultured on Violet Red Bile lactose agar at 37 °C for 24–48 h, aerobically. The LAB were cultured on MRS (Main Rogosa agar), MSE and APT agar at 30 °C in microaerophilic conditions. The microscopic fungi and yeasts were cultured on Malt extract agar at 25 °C for 5 days, aerobically. Isolated strains (total 669) were subjected to identification by the MALDI-TOF MS. Among total count the identified bacteria mostly were Acinetobacter baumannii, Bacillus cereus, Micrococcus luteus and Staphylococcus warneri. Escherichia coli and Enterobacter cloacae were the most abundant coliform bacteria representatives identified. Coliform bacteria included Citrobacter, Hafnia and Klebsiella. Altogether three genera belonged to the LAB – Lactobacillus, Lactococcus and Leuconostoc were identified with Lactococcus lactis, Lactobacillus plantarum, Lactobacillus coryniformis, L. fructivorans and Leuconostoc mesenteroides were considered as the dominated LAB species in dairy products. Among yeasts, Kluyveromyces lactis, Candida zeylanoides and Yarrowia lipolytica were among the most isolated.

  • Awareness and adoption of a nurse sow management system among small-scale pig farmers in Nakuru County, Kenya
    113-120
    Views:
    155

    This study aimed to assess the level of awareness and adoption of nurse sow management strategies among small-scale pig farmers in Nakuru County, Kenya. The research included a survey of pig farmers who visited the Nakuru Agricultural Show in July 2023. A total of 139 farmers were interviewed within 5 days of the show. The corresponding author interviewed the respondents in a face-to-face engagement, where questions on nurse sow management were initially drafted in English and translated into Kiswahili, i.e. the second language in Kenya. The obtained results indicated that farmers came from two main regions of the county: the northern part (54.7%, 76/139) and the southern part (45.3%, 63/139). Levels of awareness and adoption were insignificant between the two groups (χ2, p<0.05). A major obstacle to the adoption of this strategy was identified as feed challenge at 77.7%; (108/139) and market issue at 59.7%; (83/139). This study identified important aspects and limitations that should be considered when developing sustainable productivity development strategies for Kenyan pig farmers. To assist the sustainable growth of small-scale pig production, the authors suggest government measures that shield farmers against exploitation of feed and marketing components, as well as advocating for effective breeding to increase live born.

  • Evaluation of Hungarian show-jumping results using different measurement variables
    81-85
    Views:
    190

    The aim of the study was to compare different fitted models for show-jumping results of sporthorses and to estimate heritability and repeatability value. Show-jumping competition results collected between 1996 and 2011 were analyzed. The database contained 358 342 starts of 10 199 horses. Identity number, name and gender of the horse, rider, competition year, the level and location of the competition and placing were recorded in the database. To measure performance of horses, placing, number of starters and competition level were used. Competitions were categorized into five groups based on their difficulty level. The used repeatability animal model included fixed effects for age, gender, competition place, year of competition (and competition level in case of non-weighted measurement variables), and random effects for rider, animal and permanent environment effect. Variance components were estimated with VCE-6 software package. The goodness-of-fit of the models was low and moderate. Heritability and repeatability values were low for each measurement variables. The best goodness-of-fit model the weighted square root of placing resulted the highest heritability and repeatability value h2=0.074 and R=0.296.