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HPLC-MS analysis of the active ingredients of chamomile (Matricaria chamomilla) bee feeding syrup
61-65Views:300Apiarists let prepare by bee honey products containing medicinal drugs. Our aim was to prove that the active ingredients originated from the herbs are also present in the bee products. This publication focuses only to the chamomile (Matricaria chamomilla) containing feeding syrup and the difference between the various syrup phases. To fulfil this task, we developed a method analysing the non-volatile components of the syrup using the flowers of chamomile. The method involves a filtration followed by HPLC-MS analysis. The analyses and the data evaluations proved that the non-volatile components of the herb were transferred to syrup. As the characteristic components of certain herbs could be identified also in the syrup, it is obvious that the components responsible for the medicinal effects are also transferred.
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The changes of the purple coneflower’s (Echinacea purpurea L.) herb and radix drug yield under different fertilization conditions
79-83Views:210In this research, the purple coneflower’s (Echinacea purpurea L.) nutrient requirement was examined under different fertilization conditions in a small plot experiment. We measured the medicinal plant’s raw and dry herb and root drug mass and drying loss under different fertilization settings and meteorological factors.
From the drug’s raw and dry mass perspective, based on the results, in our opinion, the control setting exceeded all nutrient settings’ results in 2016. In 2017 and 2018, the N75P100K150 nutrient setting has the biggest herb yield. As for the root yield, it was also the N75P100K150 setting which produced the biggest yield.
The Pearson's correlation test was performed to investigate the connection between the quantity of the raw, the dried herbs, the different nutrient settings and meteorological factors.
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Impact of chronic heat stress on digestibility of nutrients and performance of meat type ducks
51-55Views:215The aim of the study is to determine the effect of vitamin and mineral supplement under permanently high environmental temperature (30±1 °C) on the digestibility of nutrients, performance and furthermore the composition of duck meat in the growing period. A total of four hundred mixed sex 14 days old Cherry Valley type hybrid ducks were used for the study. Two experimental diets were formulated in the study (control and vitamin E, C and zinc supplemented diet). Based on the results the following conclusions were drown: the antioxidant defence system plays an important role in the reduction of heat stress generated lipid peroxidation process. Feed additives which have direct or indirect antioxidant effects can reduce the negative effects of heat stress on the ducks performance and meat composition. Digestibility of nutrients (Dry Matter, Crude Protein, Crude Fat) was not affected by antioxidant supplementation under chronic heat stress (30±1 °C). The performance was affected significantly by Vitamin C and E and zinc supplementation under heat stress (P<0.05). In the treated group the daily weight gain (dWG) increased and the feed conversion ratio (FCR) was improved significantly (P<0.05). The energy and protein conversion was decreased also significantly (P<0.05).
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Movement of Particles in the Air
22-26Views:117The physical characteristics of particles (seeds and fertilisers) can strongly influence their movements both in seeding and spreading machines and in the air. It is therefore essential to study these particles when constructing such machines. In this respect the size, shape, coefficient of friction and aerodynamic resistance of particles are of great importance.
Due to their irregular shape, determining the size of particles is a troublesome process. A precise description of particles has to include several sizes and can be obtained from their screen size. Many physical properties of particles are relevant during movement in the air, but the aerodynamic resistance coefficient is the most important (Hofstee et al., 1990). Two types of wheat and four types of fertiliser particles were investigated (supported by the National Scientific Foundation OTKA, T-026482). An elutriator was designed and constructed (Csizmazia et al., 2000), in which an airflow is supplied by a centrifugal fan. Air velocity was measured with a thermal sensor. Particle sizes, mass and terminal air velocity were measured. The influence of the aerodynamic resistance coefficient on the particles’ motion is also discussed. -
Daily behaviour of Hungarian Grey Cattle under range grazing conditions
45-48Views:198Cattle behaviour on rangeland depends on external factors, such as grass allowance and quality, temperature, net solar radiation, distance from water-source, wind speed and direction, air pressure changes and the applied breeding technology. Our research is based on previous empirical observations and modern methods to analyze the behaviour of the Hungarian Grey Cattle. Today it is crucial to use cost-effective solutions in modern beef cattle farming therefore we introduce a cost-efficient method to study and follow cattle herds. We are studying relation between traveled daily distance and air pressure. The various weather fronts influence behavioural characteristics and traveled daily distance. According to our hypothesis, the pressure-change and the wind direction has significant effect on cattle activity on pasture. As the different air masses alter the barometric conditions and unbalance the neuroendocrine system, indirectly cause relaxed or agitated behaviour
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Phytoplasma infection status survey in plum psyllid (Cacopsylla pruni) population
45-48Views:160European Stone Fruit Yellows (ESFY) phytoplasma disease causes an increasing amount of damage. This is especially true to the Gönci growing region. The insect vector of the disease, which has been shown to have a vector role during transmission experiments, is the plum psyllid (Cacopsylla pruni). In 2018, during the swarming period of plum psyllid, in 3 different settlements (Boldogkőváralja, Nagyvárad and Bekecs) 265 psyllidswere collected, from which 165 were plum psyllid. Molecular biology studies showed 106 individuals infected with phytoplasma, of which 20 were males and 86 were females.
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Variability examination of photosynthetic pigment content and specific leaf area in individual maize (Zea mays L.) plants
153-157Views:251Currently, maize is one of the most important crops (Zea mays L.) both globally and in Hungary. We compared physiological parameters of a maize genotype – p9903 – at two different experimental sites in a field experiment. Furthermore, we examined these parameters’ variability in individual plants on the leaves with different ages. Absolute chlorophyll content of the leaves were analysed, separately that of chlorophyll a and chlorophyll b. We also measured the absolute carotenoid contents of leaves. Furthermore, we calculated these photosynthetic pigments’ content ratio. Specific leaf area (SLA) and dry matter weight were also measured in order to characterise plant production. The results obviously reflect the decreasing in the efficiency of photosynthetic apparatus on the low yield site. Otherwise, we identify significant differences only in certain cases of leaves.
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Determining elements of variety-specific maize production technology
157-161Views:82Our aim was to work out such new maize fertilizer methods and models which can reduce the harmful effects of fertilization, can
maintain the soil fertility and can moderate the yield fluctuation (nowadays 50-60 %).
The soil of our experimental projects was meadow soil. The soil could be characterized by high clay content and pour phosphorus and
medium potassium contents. In the last decade, out of ten years six years were dry and hot in our region. So the importance of crop-rotation
is increasing and we have to strive for using the appropriate crop rotation.
The yields of maize in monoculture crop rotation decreased by 1-3 t ha-1 in each dry year during the experiment (1983, 1990, 1992,
1993, 1994, 1995, 1998, 2000, 2003, and 2007). The most favourable forecrop of maize was wheat, medium was the biculture crop rotation
and the worst crop rotation was the monoculture.
There is a strong correlation between the sowing time and the yield of maize hybrids, but this interactive effect can be modified by the
amount and distribution of precipitation in the vegetation period. At the early sowing time, the grain moistures were 5-12 % lower compared
to the late sowing time and 4-5 % lower compared to the optimum sowing treatment.
There are great differences among the plant density of different maize hybrids. There are hybrids sensitive to higher plant density and
there are hybrids with wide and narrow optimum plant densities.
The agro-ecological optimum fertilizer dosage of hybrids with a longer season (FAO 400-500) was N 30-40 kg ha-1 higher in favourable
years as compared to early hybrids.
We can summarize our results by saying that we have to use hybrid-specific technologies in maize production. In the future, we have to
increase the level of inputs and have to apply the best appropriate hybrids and with respect to the agroecologial conditions, we can better
utilize the genetic yield potential. -
Mitochondrial DNA-based diversity study of Hungarian brown hares (Lepus europaeus Pallas 1778)
23-29Views:171The brown hare being an important game species which is widespread across the European continent has been in focus of many population genetic studies. However only a few comprising researches can be found on the diversity of Central-European populations.
The aim of our large scale long term ongoing study is to fill this gap of information on the species by describing the genetic history and structure of the brown hare populations of the area using both mitochondrial DNA markers and genomic skin and hair colour regulating genes.
This article gives forth a part of our results concerning the mitochondrial DNA diversity of Hungarian brown hares based on amplification of a 512 bp long D-loop sequence. N=39 tissue or hair samples have been collected from 15 sampling sites on the Hungarian Great Plain. We have described a high level of haplotype diversity (Hd=0.879±0.044) based on a 410 bp alignment of our sequences. We have found 17 haplotypes within our sample set with the nucleotid diversity of π=0.01167±0.0022. Our ongoing research shows high genetic diversity for the brown hare in the studied region and a second alignment with 156 sequences downloaded from GenBank indicates a geographic pattern of haplotypes among the studied populations though these results need confirmation by our further analyses.
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Evaluation of population parameters and biometric data of an Eurasian Collared Dove (Streptopelia decaocto Friv.) population in the Great Plain of Hungary
89-92Views:361The Eurasian Collared Dove shows different patterns in density all over Hungary. In some cases, the population has decreased to a lower level, but there are habitats where the population remained stable. Several factors were taken into consideration during the evaluation of this phenomenon. These publications state that the increased urbanization of corvids, the lack of food sources, and the changes in nesting sites caused the decrease in numbers. During our research, we intend to contrast the differences in population structure of Collared Doves that differ from each other in the density of predators presented at nesting sites. As a first step, in this paper, numerous samples (n=118) were taken from a population that is less influenced by corvid species’ nest predation. Our prediction was that the population parameters remained at the level of 1980’s, in which the population of Eurasian Collared Dove has reached its maximum, according to further publications.
During the evaluation process, our data were collected from hunting bags near Nyíregyháza. Results were compared with former publications. We stated that the population structure, such as age and gender distribution remained stable in the evaluated population. In addition, morphometric factors of the collected individuals were also measured, such as weight, wing length, 3rd primary feather, and tail length, which were compared with former surveys. During statistical tests no significant difference was revealed among population parameters, but there were significant differences between most of the morphometric data, (p>0.05).
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Evaluating changes in nitrogen and sulphur content in a soil-plant system in a long-term fertilization experiment
77-85Views:152The objective of this study was to evaluate the impact of long term NPK fertilization (considering that S containing superphosphate was supplied for 26 years of experiment, but since 9 years S has not used any longer) on sulphur- and nitrogen content and N/S ratio of winter wheat. The second objective of this work was to determine the changes of the amount of the different nitrogen and sulphur fraction in chernozem soil in a long term fertilization experiment. The third aim of the work was to determine if a relationship could be established between the studied parameters. Based on our results, it can be stated that the sulphur containing superphosphate supplied in the period of 1984-2010 has no longer significant effect on total sulphur content of plant in 2018. The NPK fertilization treatments had positive effect on total nitrogen content of winter wheat. In general, increasing NPK doses resulted in significantly higher nitrogen. The effect of irrigation applied in previous years has no statistically significant effect on the sulphur and nitrogen content of wheat. The wheat grain produced in our experiment, especially in fertilized treatments showed S deficiency. Analysing the changes of CaCl2 soluble nitrate-N and total N of the soil, it can be stated that the effect of increasing fertilizer doses clearly appears in these parameters, because the treatment with increasing fertilizer doses resulted higher CaCl2 soluble N forms compared to the control treatment in soil. These values increased until flowering stage of wheat and after that a slightly decrease was observed as a result of higher N uptake of plant. In overall, it can be stated, that the effect of superphosphate on measured sulphur fraction is prevailed. With increasing fertilizer doses higher sulphate content was detected in soil, but the sulphate content measured in different soil extractant is not enough for the wheat in this experiment area. Studying the correlation between the measured parameters of plant and soil, it can be concluded, that the relationships between nitrogen in the plant and in the soil is stable, and did not change during the growing season. The correlation between plant S and soil S varied in the measured periods and the r value was low in most cases. At the stage of flowering the highest r value was found between KCl-SO4 and plant S. In the stage of ripening the strongest correlation was detected between KH2PO4-SO4 and grain S content.
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Preliminary Critical P-limit Values of 0.01 M CaCl2 Soil Test Procedure
18-21Views:119In the last decade, the 0.01 M CaCl2 extraction procedure was tested as a multi-nutrient extractant. In 1995-97, international joint research activities were carried out within the COPERNICUS project. Detailed calibration of conventional and the 0.01 M CaCl2 extraction procedures for pH, Mg and K were published.
The amount of phosphorus extracted using a 0.01 M CaCl2 solution is very low and reflects the intensity parameter of phosphorus bio-availability. As a readily desorbed P fraction of soils can reflect the soil P-supply and the CaCl2-P values are in close correlation with P-fertiliser rates and P balance. However, the effects of various soil characteristics on CaCl2-P values are different and their interpretation is difficult.
Relatively poor correlations were found between amounts of P extracted by conventional and CaCl2 soil test methods and, therefore, P limit values could not be calculated directly. To characterise the soil P supply at different sites, the CaCl2 desorbed P and the adsorbed P in a modified Baker Soil Test were also applied.
Soil test results of Hungarian long-term fertiliser experiments and recommended CaCl2-P limit values, calculated on yield effects and soil characteristics, are discussed. -
Promising Leuce poplar clones in sandy ridges between the rivers Danube and Tisza in Hungary: a case study
111-113Views:247An intensive integrated research and development work has been carried out on the improvement of Leuce poplars including primarily the native white poplar (Populus alba L.) and its natural hybrid grey poplar (Populus × canescens). More than 70 percent of the Leuce poplar stands can be found on calcareous sandy sites in the Danube–Tisza region, so they play a significant role in the poplar management of this part of the country. The most important task ahead of Hungarian poplar growers is to improve the quality of poplar stands and plantations based on selecting new clones and cultivars. The growth and yield of four promising Leuce poplar clones was evaluated on a marginal site in central Hungary. The clones ‘H 425-4’ (Populus alba L. × Populus alba L.), and ‘H 758’ (Populus alba L. Mosonmagyaróvár 124) seem to be suitable for wood production, while the ‘H 427-3’ (Populus alba L. × Populus alba L. cv. Bolleana) and ‘H 422-9’ (Populus alba L. × Populus grandidentata (Michx)) clones (with decorative stem form) could be better used for tree lines and ornamental plantations.
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Investment funding programs on dairy farms in Hajdú-Bihar County
11-16Views:181The dairy farming sector needs constant modernization of its buildings, machines and technology in order to maintain its competitiveness and productivity. For the greater scale investments, these companies try to apply for state funds in addition to their own financial sources. Therefore, the investment support system plays a great role by improving the competitiveness and modernity of these farms. In the last 10 years, 7 funding programs were available for dairy farms. In this research, 25 dairy farms were interviewed in Hajdú-Bihar County. The examined farms cover more than 50% of the milk production in Hajdú-Bihar County. The survey questions focused on the investment funding program use of farms between 2007 and 2017 and renewable energy investments including all programs. The results show how many tenders were submitted, how many were successfully evaluated and completed. All farms had at least two successfully evaluated projects. A correlation was found between completed projects by farm size and completed projects. The connection of the modernity index of dairy farms and the completed projects was also shown.
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Effect of allithiamine on the level of hyperglycaemia-induced advanced glycation end products
41-44Views:291Diabetes mellitus is a rapidly growing public health burden in both developed and developing countries. Diabetes mellitus is accompanied by hyperglycaemia, which can cause tissue injury by several mechanisms. One of these is the formation of advanced glycation end-products (AGEs). In this study, the effect of allithaimine, a fat-soluble thiamine derivative, was investigated on hyperglycaemia-induced AGEs levels using human umbilical cord vein endothelial cells (HUVECs) as a hyperglycaemic model. HUVECs were isolated by enzymatic digestion, characterized by flow cytometer and treated 30 mM glucose plus allithaimine or thiamine or cell maintenance medium as control. Allithiamine was synthesized and purified. The structure of the synthesized and isolated compound was verified by reverse phase HPLC and MALDI-TOF. AGEs were evaluated by ELISA. Collectively, our results indicate that allithiamine can reduce level of the hyperglycaemia-induced AGEs similar to thiamine.
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The Natural, Social and Economic Conditions and Opportunities for Development of Balmazujvaros, Especially in the Case of the Co-operative Kvaliko
77-85Views:98Balmazujvaros as a settlement near Hortobagy has to cope with both advantages and disadvantages. Its natural and social conditions are mainly given, the agricultural characteristic is dominant, and the number of the employed is the highest in the agricultural firms and processing industry. Developing the third sector, including hosting, tourism, eco-tourism, thermal-tourism, may be an opportunity for the city. The subsidy for rural development relating to the SAPARD-programme may contribute to this, as the city is the member of the Association of Hajdu Towns.
The agriculture plays an important part in Balmazujvaros in which production and marketing of vegetables and fruits excel. Several farmers realised this opportunity and founded the Marketing Co-operative of Vegetable Producers in Eastern Hungary, that is the Kvaliko. This PO works in a Corporation form and is constantly developing, spreading and expanding its choice making use of the local natural, social and economic conditions and applications for realising its investments. This PO may set an example for the other entrepreneurs and farmers of Balmazujvaros to co-operate and it may be considered as an already realised opportunity. -
A Critical Review of Selected Computer Assisted Language Testing Instruments
3-6Views:170Over the past few decades, a fairly large literature examining the effectiveness of computer-assisted language learning (CALL) has been developed. The findings indicate that language learners have generally positive attitudes toward using computers in the classroom. Less is known, however, about the more specific areas of computers in language testing. The purpose of this article is to examine recent developments in language testing that directly involve computer use. After a brief overview of computer-based testing (CBT) in general, web-based testing (WBT) is defined and certain issues reviewed.
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Classification of a diffuse heavy metal polluted mining site using a spectral angle mapper
119-123Views:128Characterization of heavy metal polluted abandoned mining sites is complicated, as the spatial distribution of pollutants often changes dramatically.
In our study, a hyperspectral data analysis of the Gyöngyösoroszi abandoned Pb-Zn mine, located in northern Hungary, where Záray (1991) reported serious heavy metal contamination, was carried out using ENVI 4.3. In this area, galena (PbS), goethite (FeO(OH)), jarosite (KFe3(SO4)2(OH)6), sphalerite ((Zn, Fe)S) and pyrite (FeS2) were the predominant minerals in the alteration zones was chosen as the target mineral.
Spectral angle mapper (SAM) and BandMax classification techniques were applied to obtain rule mineral images. Each pixel in these rule images represents the similarity between the corresponding pixels in the hyperspectral image to a reference spectrum.
As a result of hyperspectral imagery the distribution of pyritic minerals (sphalerite, galena) in the area was defined. Both of the mineral formations occur, especially in mine tailings, the area of the ore preparatory, and the Szárazvölgyi flotation sludge reservoir. According to the results, jarosite and goethite have similar distributions to sphalerite and galena. The results showed that hyperspectral remote sensing is an effective tool for the
characterization of Pb, Zn and Fe containing minerals at the examined polluted sites and for modelling the distribution of heavy metals and minerals in extensive areas.
This classification method is a basis of further detailed investigations, based on field measurements, to map the heavy metal distribution of the studied area and to quantify the environmental risks caused by erosion, which include DEM (digital elevation model) and climatic and hydrological data sources. Furthermore, it can be used primarily to support the potentially applicable phytostabilization technique and to isolate hot spots where only ex-situ remediation techniques can be applied. -
Effect of the media on morphology of Cryphonectria parasitica (Murr.) Barr isolates and their Vegetative Compatibility Groups
38-42Views:176The most dangerous pathogen for the European and American chestnuts is the blight fungus Cryphonectria parasitica (Murrill) Barr. Short after its introduction a big number of chestnut trees were destroyed on the infested area. The control could be really complicated, because of the numerous vegetative compatibility groups of the fungus. There is a type that carries a mycovirus viz. hypovirus in the cytoplasm. We are able to control effectively this pathogen by using mycovirus-carrying strains (called hypovirulent fungal strains also). In laboratory it is easy to multiply the virulent and the hypovirulent strains of the fungus but do not easy to differentiate colonies visually on simple PDA medium. During our research, we tested different types of media, based on potato and chestnut bark extract respectively. It was observed that on potato medium the virulent strains produce more orange pigments. So it is more easy to differentiate virulent or hypovirulent isolates of chestnut blight fungus based on colony colour and morphology.
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Comparative study of a winter wheat variety and hybrid sown after different pre-crops on chernozem soil
63-69Views:244Wheat production is a determining branch within Hungarian crop production (produced on nearly one million hectares). Weather anomalies caused by climatic change confirmed the importance of the biological background (variety, hybrid) in wheat production. The adapting ability and reaction of different wheat genotypes towards nutrient supply were studied in a long-term field experiment on chernozem soil type in the case of different pre-crops (sunflower and maize). According to the experimental results of the vegetation of 2017/2018, the yield of the variety Ingenio sown after the sunflower as previous crop ranged between 4168 and 8734 kg ha-1, while in the case of maize as previous crop, this value ranged between 2084 and 7782kg ha-1, depending on the applied nutrient supply level. The studied genotypes produced rather significant yield surplus as a response to the application of mineral fertilization (4.6–5.1 t ha-1 after sunflower and 5.7–6.3 t ha-1 after maize). Optimal mineral fertilizer dosage was determined by both the genotype and the pre-crop. N-optimum values of wheat genotypes was determined using regression analysis. In the case of the variety Ingenio sown after sunflower, the optimum range was N144-150+PK, while after maize, it was
N123-150+PK, respectively. For the hybrid Hyland, these optimum ranges were N114-120+PK, just as N150-153+PK, resp. The application of optimal mineral fertilizer dosages improved water utilization of the studied wheat genotypes to a significant extent. WUE values of the control, unfertilized treatments ranged between 4.1–8.3 kg mm-1, while in optimal fertilizer treatment, it ranged between 15.5 and 17.4 kg mm-1. -
Evaluation of the correlation between SPAD readings and absolute chlorophyll content of maize under different nitrogen supply conditions
121-126Views:422Currently, one of the most important objectives of agriculture is to maintain the principles of the sustainability. The use of precision technologies in agriculture belongs to this topic. The use of precision technologies is increasingly widespread in the cultivation of various agricultural crops, including maize. Sensing is an important part of these techniques. In our experiment we compared two methods: measuring relative chlorophyll content and the method of determine the extractable chlorophylls. The experimental plant was maize (Zea mays L.) and the measurements were performed at an early development stage (V8) of three genotypes. Three levels of nitrogen (0; 80; 160 kg ha-1) were applied during the experiment. The relative chlorophyll content was measured by SPAD-502 (Minolta, Japan) and a handheld GreenSeeker (Trimble, USA) device. The extractable total chlorophyll content decreased in parallel with the increased nitrogen level. The obtained SPAD values were diversified furthermore the NDVI values have not been changed for the effect of different nitrogen fertilization. In the early stages of development of maize, these parameters need to be complemented with other measurements to provide reliable information about the crops nitrogen status.
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Comparison of Variability among Irradiated and Control Inbred Maize Lines via Morphological Descriptions and Some Quantitative Features
70-73Views:78Knowledge of genetic diversity in breeding material is fundamental for hybrid selection programs and for germplasm preservation as well. Research has been done with nine irradiated (fast neutron) and four non-treated inbred lines. The aims of this study were (1) to investigate the degree of genetic variability detected with morphological description (based on CPVO TP/2/2) in these materials, (2) to compare the genetic changes among irradiated and non-irradiated maize inbred lines (based on some quantitative features). The irradiation did not change any of the characteristics clearly in positive or negative way, which can be related to the fact that the effect of induced mutation on genetic structure cannot be controlled. From the irradiated lines we have managed to select plants with earlier ripening times and better phenotypes. We could distinguish 3 main groups by the morphological features; these results match our expectations based on pedigree data. Markers distinguishable on the phenotypic level (e.g. antocyanin colouration, length of tassels) were significant in all lines.
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Analysis of different fertilization settings’ effect in the case of the summer savory’s (Satureja hortensis L.) yield and active agents
101-105Views:180During our research we investigated the Mediterranean summer savory’s (Satureja hortensis L.) nutrient requirement with different fertilization settings in a small plot trial. We measured the medicinal plant’s raw and dry drug mass, and the presence of the main essential oil active agents under the different fertilization settings using SPME (Solid phase microextraction) and GC-MS (Gas Chromatograph mass spectrometer).From the drug’s raw and dry mass perspective, based on the obtained results, in our opinion, the N30P40K60 nutrient requirements were ideal in 2015, while the N60P80K120 nutrient requirement showed the best values in 2016. A significant relationship is assumed between the different fertilization settings and the essential oil active agents’ distribution. During the analysis of data, we compared three of the savory’s essential oil active agents’ percentage of presence in the nutrient requirement samples. -
Development of an InDel marker set to establish hybridization between wild boar and domestic pig (Sus scrofa) breeds
21-25Views:308Wild boar and domestic pig breeds belong to the same species (Sus scrofa), so they can easily have viable offspring. This could be a problem in preserving the genetic lines of wild boars, keeping clean the food industry from lower-grade hybrid boar meat, and „producing” ethically questionable trophies, too. The aim of our study was to develop a cost-efficient, fast, easy and accurate marker set which can separate the wild boars from hybrids and domestic pig breeds.
The InDel markers were developed using 59 full pig genomes of 17 different breeds (e.g. Duroc, Large White, Landrace, Mangalica, wild boar). Sequence differences between the genomes of wild boars and domestic breeds were identified in variant call files, and verified using the IGV software. Wild boar, mangalica and duroc specific primers to amplify the chosen InDel regions were designed using Primer3.
After preliminary tests five markers were chosen, three wild boar specific, one Mangalica specific and one Duroc specific one. Fluorescently labelled primers were used to make the valuation easier and more accurate with capillary electrophoresis instead of gel-electrophoresis. The markers were optimised individually and in multiplex conditions and tested in samples of 11 breeds.
In conclusion, a new, faster and cheaper set was developed to separate the wild boars from the hybrids and domestic breeds. Based on the preliminary testing on wild boars, duroc and mangalica breeds zero samples resulted false negative, so it is 100% accurate. In addition, it is a much more cost- and time-effective way than testing every single sample with STR sets.
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Physiological traits and yield of three soybean (Glycine max (L.) Merr.) genotypes as affected by water deficiency
11-15Views:217Soybean is a very important legume; it has the highest protein content, and it is a very important source of vegetable oil. Soybean is droughtsusceptible, and drought is one of the major abiotic stresses that has been increasing over the past decades as a result of the global climatic changes. To evaluate the influence of drought stress, three soybean genotypes were grown under rainfed conditions, and compared to irrigated controls. The obtained results showed that the chlorophyll content, leaf area index and plant height decreased under drought stress conditions, which led to noticeable and sometimes significant yield reduction. Our results suggest more specific studies on the physiological changes of the local soybean genotypes under drought stress to better select the adopted ones.