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  • Heterobeltiosis and combining ability among Egyptian cotton genotypes under well irrigated and water deficit conditions
    5-18
    Views:
    336

    Two field experiments were carried out during the 2014 and 2015 seasons to evaluate certain genotypes of Egyptian cotton under well irrigated and water stress conditions. The cross combination Giza 69 x Australy recorded the best values for better parent heterosis for all physiological measurements and earliness index under well irrigated and deficit conditions. Of the female parents, Giza 86 was found to be a good general combiner for most yield characters under both normal and stress conditions, followed by Giza 94. Data illustrated that Giza 45 was the best general combiner for most fiber quality traits under both conditions. The cross combinations Giza 86 x Dandra and Giza 69 x Pima S6 showed significant positive SCA values for seed cotton and lint yield/plant under well irrigated and water deficit conditions. However, the combinations Giza 77 x PimaS6 and Giza 94 x 10229 recorded significant positive SCA effects for seed cotton yield/plant under stress. Whilst, the cross Giza 68 x 10229 recorded significant desirable SCA effects for most fiber quality properties. In this context, the estimates significant positive general and specific combining ability effects indicated that the epistasis and/or dominance effects for F1 hybrid in cotton could be important to a certain extent.

  • Testing the antimicrobial activity of essential oils
    71-74
    Views:
    272

    The vapor phase of some essential oils proved to have antimicrobial activity. Utilization of the vapor phase of Eos is presently understood as one of the possible alternatives to synthetic food preservatives which could be used in the future. However, testing the vapor phase of EOs against microorganisms causing food-borne diseases (e.g. Salmonella enteritidis or Staphylococcus aureus) or food spoilage is relatively new. Consequently, due to the large number of known EOs, research on their antimicrobial activity is still largely in the phase of in vitro rather than in vivo testing. Moreover, no standard and reliable method for fast screening of a wide range of samples exists. Thus, the aim of this study is to show results concerning tests of the antimicrobial activity of EOs against S. enteritidis or S. aureus, which were conducted by two modifications of the disc volatilization method we developed. The lately developed method has the potential to become widely used for fast screening of EO antimicrobial activity in the vapor phase.

  • The effect of breed and stage of lactation on the microbiological status of raw milk
    37-45
    Views:
    700

    The microbiological quality of the milk is important not only for food safety, but it can also influence the quality of dairy products. The microbiological status of raw cow milk can be influenced by many factors. Our aim was to determine whether there was a difference between the microbiological quality of milk of two different cow breeds (Holstein Friesian and Jersey) kept and milked in the same conditions, and how the microbiological quality of the raw cow milk changed during lactation (beginning, mid, and end). Samples were taken and analysed in July, August and September in 2018 from two dairy farms in Hajdú-Bihar county. During the conducted studies, the total plate count (TPC), the coliform count, the Staphylococcus aureus count and the coagulase-negative Staphylococcus (CNS) count of raw milk samples were determined.

    There was no significant difference (P>0.05) between the milk of the Holstein Friesian and Jersey breeds in the case of TPC. However, the mean coliform count of milk samples taken from Holstein Friesian cows was significantly lower (P<0.05) than the mean coliform count of milk samples taken from Jersey cows. S. aureus was detected in one of the twelve milk samples taken from Holstein Friesian cows, and in two of the eleven milk samples taken from Jersey cows. CNS was found in larger amount in milk samples taken from Holstein Friesian cows, and the difference was significant (P<0.05). Both TPC and CNS count were significantly higher (P<0.05) in individual milk samples taken at the end stage of lactation, than in samples taken in the earlier stages of lactation from Farm “A”. However, in the case of Farm “B”, there was no significant difference (P>0.05) in colony counts at different stages of lactation. S. aureus was only present in milk samples that collected from cows, which were at the beginning and middle stages of lactation. Testimg the hemolysin production ability of S. aureus strains isolated from the raw milk samples, only weak hemolysis was observed on blood agar. In case of antibiotic resistance testing, it was found that all strains were susceptible to cefoxitin, chloramphenicol, clindamycin, erythromycin, gentamicin, penicillin G, tetracycline and trimethoprim/sulphamethoxazole.

    Based on the results of our studies, staphylococci were detected in a higher amount in the milk of Holstein Friesian cows, and coliform bacteria were detected in a higher number in the milk of Jersey cows. Summing up the results of the milk samples taken from the different stages of lactation in one of the farms, it can be concluded that higher TPC and CNS count could be detected at the end stage of lactation than in the samples taken from the earlier stages of lactation. The fact that at the end of lactation the microorganisms could be detected in a higher colony count may be related to the fact that teats could be damaged during lactation by the milking machine, which increased the chance of imvading the microorganisms into the udder.

  • Genetic diversity of the Hungarian draft horse assessed by mitochondrial DNA
    29-32
    Views:
    664

    Hungarian draft is a horse breed with a recent mixed ancestry. It was developed in the 1920s by crossing local mares with draught horses imported from France and Belgium. To genetically characterize the breed and to set up the basis for a conservation programme, we have employed a molecular marker: a 256-bp D-loop mitochondrial DNA fragment. We analyzed 124 horses representing Hungarian draft horses to assess the maternal phylogeography of the breed. Sequence analysis of a 256-bp segment revealed a total of 34 haplotypes with thirty-four polymorphic sites. High haplotype and nucleotide diversity values (Hd=0.953±0.001; π=0.024±0.001) were detected. The average number of pairwise differences were k=5.998. This breed counts 800 mares today, and only survive due to breeding programmes, this way each haplotype frequency depends on the extent to which mares are involved into the breeding. The reduced number of surviving maternal lineages emphasizes the importance of establishing a conservation plan for this endangered breed. Due to the revealed 34 polymorphic sites we could presuppose twelve maternal linages, which could be a first step for making a breeding programme.

  • Growth and yield patterns of black locust (Robinia pseudoacacia L.) sample trees affected by site conditions: case studies
    125-128
    Views:
    334

    The trees removed from the long-term experiment plots are available for the measurements as lying trees. Through the determination of the volume in sections along the stem, the stem form, the stem volume and other factors can be specified. The comparison of the stems of individual trees of first and third yield classes of black locust (Robinia pseudoacacia L.) stands shows that site conditions have a main effect on the yield (mean tree volume). The difference can be as high as 53% at the age of 30 depending on the sites. To determine the growth patterns based on tree volume is rather a new approach in the light of the relevant literature. The obtained results also highlight the importance of choosing the appropriate tree species for a given site.

  • Investigation of combining ability and superiority percentages for yield and some related traits in yellow maize using line × tester analysis
    5-14
    Views:
    404

    Combining ability estimation is an important genetic attribute for maize breeders in anticipating improvement in productivity via hybridization and selection. This research was carried out to investigate the genetic structure of the 27 F1 maize hybrids established from nine lines derived from Maize Research Department and three testers, to determine general combining ability (GCA), determine crosses showing specific combining ability (SCA) and superiority percentages for crosses. Nine lines, three testers, 27 F1 hybrids and two check commercial hybrids (SC162 and SC168) were studied in randomized complete block Design (RCBD) with three replications during 2016. The results of mean squares showed that significant and highly significant for most studied traits (days to 50% tasseling, days to 50% silking, plant and ear height, ear position, ear length, no. of kernels per row, 100-kernel weight and Grain yield). Estimates of variance due to GCA and SCA and their ratio revealed predominantly non-additive gene effects for all studied traits. Lines with the best GCA effects were: P2 (line 11) and P6 (line 21) for grain yield, for testers Gm174 and Gm1021 had significant GCA effects for grain yield. The hybrids P5×Gm1021, P6×Gm1021, P7×Gm1021, P8×Gm1002, P9×Gm1002 had significant and negative SCA effects for grain yield. Crosses P1×Gm174, P2×Gm1002, P5×Gm1021, P6×Gm174, P6×Gm1021, P7×Gm1021, P8×Gm1002, P9×Gm1021 were the best combinations manifested and significant superiority percentages over than check varieties (SC162 and SC168) for most studied traits. Therefore, these hybrids may be preferred for hybrid crop development.

    Abbreviations: GCA general combining ability; SCA specific combining ability

  • The Effect of Fertilization and Irrigation on Maize(Zea mays L.) Production
    26-29
    Views:
    424

    In a long-term field experiment set up at the Látókép experimental station of the Center of Agricultural Sciences of Debrecen University, the data of the last five years (1995-1999) were analyzed to determine the crop production factors with the greatest influence on maize production and the relationship and interactions between irrigation and fertilization.
    In the extremely dry year of 1995, fertilization was found to cause substantial yield depression in the absence of irrigation. According to results of analysis of variance, fertilization significantly reduced the maize yield by 40-90% compared to control plots. Under irrigated conditions, there was a considerable increase in the maize yield, the yield surplus being 4.4-9.4 t ha-1, depending on the nutrient supply level.
    During the period from 1996-1999, when rainfall conditions were favorable for maize, fertilization significantly increased the maize yield even without irrigation over the average of the four years. The yield surplus due to fertilization was 3.9-4.6 t ha-1, depending on the fertilization rates. The maximum yield surplus was obtained on plots fertilized with 120 N kg ha-1, while at the rate of 240 N kg ha-1 the maize yield did not differ significantly from this value. During the period examined, corn yield was significantly higher at all three nutrient supply levels as the result of irrigation than in the non-irrigated treatment. As in the case of non-irrigated conditions, the highest fertilizer dose did not result in a substantial yield increase. An analysis of the interaction between fertilization and irrigation indicated that the yield-increasing effect of fertilization was not significantly different under irrigated and non-irrigated conditions. The significant year x irrigation interaction was confirmed by the fact that the yield surplus (1.3-2.3 t ha-1) differed greatly from the irrigation effect recorded in 1995.

  • Effects of combined nutrient supply treatments on some physiological parameters of autumn wheat
    241-251
    Views:
    304

    The Fleischmann Rudolf Research Institute in Kompolt is not only famous for plant breeding but the institute also surveys the effects of different nutrient supply methods since 1918. In 2017, we joined this research supported by EFOP 3.6.1 project. Our aim was to investigate photochemical processes – which is one of the most determinant in case of yield – of crops by in vivo field measurements. We measured the chlorophyll content of leaves using Minolta SPAD 502. We used miniPAM fluorometer to determine actual photochemical efficiency and non-photochemical quenching of PSII during natural light conditions and also to evaluate the pigment (chlorophylls and carotenoids) and water content of leaves we applied field spectrophotometer (ASD FieldSpecPro 3). We utilized these methods by various treatments (1. treatment with soil bacteria + head and base fertilizer; 2. treated by only head fertilizer; 3. treated by only base-fertilizer) in field experiment of autumn wheat (4.1–2.43–1.19 ha) in June, 2017. The difference between treatments was clearly detectable. In the case of the first treatment, physiological processes were more intense and the ripening occurred earlier. The obtained yield was the highest in the case of the area treated by soil bacterial. Based on the results, the first treatment can be recommended in practice.

  • Investigation of the impacts of the by-product of sewage treatment on some characteristics of maize in the early growth stage
    77-82
    Views:
    269

    The use of sewage sludge on arable land has been widespread for many years. This by-product, treated as waste, can provide valuable nutrients to the soil, but the applied amount of sewage sludge to arable land is limited. The possibility of application of sewage sludge is essentially determined by the composition of the sludge. The goal of the experiment was to demonstrate that the physiological, morphological, and biochemical parameters of maize (Zea mays L. cv. Armagnac) linearly change with increasing concentrations of sewage sludge (25%, 50%, and 75% as m/m%). The experiment was set up in a glasshouse. The following parameters were investigated: plant height, relative chlorophyll content, photosynthetic pigments (chlorophyll-a, chlorophyll-b, carotenoids), and leaf proline and malondialdehyde (MDA) content, and PS II quantum efficiency in the 3-leaf stages of the plants. Sewage sludge applied in lower doses had a beneficial effect on the initial growth of maize. The relative chlorophyll content was significantly higher in all treatments compared to the control. There was no significant difference in the maximum quantum efficiency of PS II reaction centers among the treatments. In this experiment, different concentrations of sewage sludge treatments had different impacts on the MDA and proline content of maize leaves. The proline content was significantly higher in all treatments, while the MDA content did not change significantly compared to the control.

  • Effect of feeds with different crude fiber content on the performance of meat goose
    5-8
    Views:
    794

    In the last 50 years, poultry meat production has increased dynamically. The role of crude fiber content in feed is unclear based on a small amount of literature about goose feeding.

    The aim of theis experiment was to determine how various crude fiber content (55; 60; 65g crude fiber/kg feed) influences the performance of meat geese in the second phase of the rearing period. 150 goslings (3 treatments, 5 cages/treatment, 10 birds/cage) were included in the test. The experiment started and finished at the age of 21st and 63rd  day, respectively.

    The obtained results suggest that various crude fiber content did not influence the performance of meat geese significantly; however, a feed of 55g crude fiber/kg resulted in better fattening results (final body weight, body weight gain, specific feed protein and energy conversion rate). In addition, compound feed of 65g crude fiber/kg proved more favourable with respect to cost-efficiency. Based on the obtained results so far, further models and farm experiments are required.

  • Experience at Russian State Agrarian University – Moscow Timiryazev Agricultural Academy on introduction and integration of precision agriculture technology
    73-76
    Views:
    198

    Traditional and precision agriculture technologies are compared on the basis of the field experiment. Problems of soil and crop survey and mapping are discussed.

  • Biological control of sweet chestnut on Pécsbánya, Hungary
    77-81
    Views:
    490

    The supervision of plant hygiene of sweet chestnut grove on Pécsbánya (South Hungary) started more than four years ago. Hypovirulent strains were applied as a biological process to control Cryphonectria parasitica fungus which causes the chestnut blight disease. By now the performed interventions have shown obvious results, the vitality of the trees has greatly improved, the amount of harvested nuts is increasing, and the hypovirulent strain has been spreading within the area. During plant health inspection the galls of chestnut gall wasp (Dryocosmus kuriphilus) was found in the year of 2015, which is the obvious symptom of new occurrence of the pest. The pest was eradicated by destroy galls, which allows taking out of consideration the damage by now in this area.

  • Investigation of harvest index influencing agrotechnical and botanical factors in hairy vetch (Vicia villosa Roth.)
    123-128
    Views:
    495

    The biggest problem of Hungarian crop farming is mass production and the simple crop rotation based on cereals. There was a decrease in sowing area of protein crops which raises crucial issues in crop rotation and land use. Therefore, growing papilionaceous plants, which are now considered to be alternative plants, should be taken under close examination. Hairy vetch (Vicia villosa Roth.) belongs to the family of papilionaceous plants and it can be grown in light weak soils.

    In Hungary, hairy vetch was used as green forage at first, but it later became a green manure plant.  Nowadays, it is used as a cover crop and its sowing seed has a good export market. In low fertile soils it is able to produce a big amount of green yield (25–40 t ha-1) even in spring while its seed yield could be 0.4–0.5 t ha-1 at farm level. In addition to its morphological characteristics hairy vetch is grown mainly with a supporting plant, i.e. triticale in many cases.

    Our purpose was to test the harvest index and its agrotechnical and botanical factors of hairy vetch in different cropping systems.

  • Will there be a horticultural triangle (cluster)? Thoughts about the reconstruction of the Hungarian horticulture between two European regions
    21-27
    Views:
    393

    The authors of this study seek the answer to the question how to develop, in the first decade or decades of the 21st century, the university-level
    horticultural scientific training, the horticultural innovation and the scientific co-operation between companies and universities in Debrecen and
    in the North Great Plain Region and – in a wider sense – in Hungary to a standard being competitive even in European terms. With the synthesis
    of the prospects of past, present and future, they drew the following conclusions. The reconstruction of agriculture, horticulture and food industry
    is a part of reforming Hungary's countryside. Horticulture, producing high added value, will be able to decisively contribute to the plan whereas
    the value presently produced in an agriculturally cultivated area of 1,000 euros/hectare can reach 2,000 to 3,000 euros in the next two decades.
    A necessary and indispensable precondition to achieving this is the strengthening of the innovation output of the Hungarian horticultural sector.
    Despite the numerous technical criticisms formulated in connection with the serious problems of Hungarian agricultural and horticultural
    scientific innovation, no progress has been made in this field for the past one and a half decade. The scientific research of this topic hardly
    continued or did not continue at all, the up-to-date surveys and in-depth analyses were missing. The objective, basic principles and tasks of the
    Act CXXXIV of 2004 (TTI) enacted concerning research-development and technological innovation are clear and progressive. The co-operation
    between the National Research Technology Office and the Hungarian Academy of Sciences, the setting up of the Innovation Fund are heartening
    opportunities. These – along with the new Higher Education Bill to be passed – may as well be suitable for restarting the Hungarian agricultural
    and horticultural scientific innovation. In our opinion, this requires a new, well-considered national agricultural programme, which can be
    conceived in the framework of the "Ferenc Entz National Horticultural Plan" proposed by us for horticulture. In the most eastern Hungarian
    university knowledge centre, at the University of Debrecen, the continuing of the horticultural scientific innovation strategy started in the last
    decade may be the focal point and generator of the development of the so-called "Hungarian Horticultural Triangle”, or "Hungarian
    Horticultural Cluster". This region comprises the Northern and Southern Great Plain Regions and the area between the Danube and Tisza
    Rivers. Here, about 70 to 75% of the total Hungarian horticultural commodity stock is produced. The objective of the HORT-INNOTECH
    DEBRECEN programme planned in 2004 by the University of Debrecen, Centre of Agricultural Science is to establish the horticultural scientific
    research-development and technological innovation structure and knowledge base of the Hungarian Horticultural Triangle / Hungarian
    Horticultural Cluster. In harmony with this, the objectives are to bring about competitive, new horticultural products, to improve the conditions
    of utilising them, to enhance the competitiveness of enterprises based on technological innovation, to make use of the research-development and
    innovation opportunities available in the regions in an efficient manner, to as full extent as possible, to encourage the creation of places of
    employment producing high added value in the field of horticulture, to improve the technical skills of those employed in horticultural researchdevelopment and to promote their enhanced recognition by the society.

  • Investigation of the production parameters, nutrient and mineral composition of mealworm (Tenebrio molitor) larvae grown on different substrates
    129-133
    Views:
    508

    During the rearing of mealworm (Tenebrio molitor) larvae, the optimisation of the growing substrate has a particular importance. The application of the appropriate substrate is a fundamental pillar for intensive and safe production. The requirements for substrate include the lack of toxins, high nutrient and micro-macro element content. The aim of this study was the evaluation of the effects of different substrates on the production parameters, nutrient and mineral composition of the mealworm larvae. The experiment lasted through 14 days. 5 treatments were set up at, where the variable was the substrate. The test system consisted of 25 units (5 treatments and 5 replicates). 10 mealworm larvae per unit, (total of 250 larvae) were used at the beginning of the experiment. Regarding the substrates, our study included sweet potato (SP), may turnip tuber (MT) and may turnip leaf (ML). In addition to the plant by-products, wheat flour (WF) and wheat semolina (WS) were used as control substrates. Trace element uptake and production parameters of the larvae were determined at the end of the experiment. Regarding the production parameters, it can be stated that the wheat semolina (0.081 ± 0.005 g) and wheat flour (0.069 ± 0.007 g) substrate used as control gave the best results for the final body weight. In terms of plant raw materials and by-products, sweet potatoes (0.063 ± 0.007) can only be recommended as substrate, while may turnip tuber and may turnip leaf produced significantly lower final body weight results (MT=0.034 ± 0.002 g ; ML= 0.036 ± 0.002 g). The nutrient composition of the larvae was not affected by the substrate, these results confirmed the high protein and fat content reported in the literature. The results with the production parameters were contradicted by the micronutrient content. Larvae reared on may turnip leaf (ML) and tuber (MT) showed the highest values for most of the macro- and microelements (potassium, calcium, magnesium, iron, zinc, copper) tested.

  • Influences of different organic fertilizers on nutrients of humic sandy soil and on the growth of Spinach (Spinacia oleracea L.)
    23-28
    Views:
    649

    A greenhouse pot experiment was conducted to compare the effects of manure with different origin (horse, cattle), various bedding materials (straw, sawdust) and diverse doses (30 t ha-1, 60 t ha-1) and the impact of food waste compost on the plant growth and the available plant nutrient content of soil. The study was conducted on humic sandy soil and consisted of 9 treatments in a randomized complete block design with four replications. Spinach (Spinacia oleracea L.) was grown as the test crop. The treatments were: 1. unfertilized control; 2. horse manure with straw (30 t ha-1); 3. horse manure with sawdust (30 t ha-1); 4. cattle manure (30 t ha-1); 5 food waste compost (30 t ha-1); 6. horse manure with straw (60 t ha-1); 7. horse manure with sawdust (60 t ha-1); 8. cattle manure (60 t ha-1); 9. food waste compost (60 t ha-1). Plant growth was monitored for 4 weeks. Shoot and root weights per pot were measured, total biomass weight per pot were counted.

    On the basis of the results it can be concluded, that among treatments the application of horse manure with straw enhanced spinach growth most significantly compared to other treatments and to the non-treated control, resulted the highest weights of leaves and roots of spinach. At the same time even small dose (30 t ha-1) of this fertilizer caused increased plant available nitrogen and phosphorus of soil and the higher dosage further increased these values. The horse manure with sawdust applied in lower dose did not alter the leaves and roots weights, but higher portion (60 t ha-1) caused significantly decreased plant biomass. The results proved that the bedding material may significantly alter the composition of manure and may change the plant nutrition effect of organic fertilizer. Cattle manure and food waste compost in both applied doses enhanced plant growth. Both fertilizers increased the plant available nitrogen forms and phosphorus content of soil, but cattle manure caused higher increase.

  • Review on the fatty acid profile and free fatty acid of common carp (Cyprinus carpio)
    99-105
    Views:
    446

    Carp or ponty in Hungarian, is considered commercial freshwater fish, which is an adaptable species in both wild and cultured conditions. Carp has high nutritional value content, favorable taste, it is rich in protein, and low in saturated fat. The nutritional content in fish is composed of many chemical constituents and influenced by many factors. One of the components that its content may be different due to internal and external factors is fatty acids, which may vary depending on endogenous and exogenous factors. The endogenous or internal factors include the genetic, size, sexual maturity, and life cycle phase. While microclimate, water quality, quality of food or diet habit, and the amount of available food or starvation are considered as exogenous or environmental factors. Freshwater fish has the ability to convert essential fatty acid into long chain polyunsaturated fatty acid like AA, EPA, and DHA. Most results showed that palmitic acid and oleic acid were the dominant SFA and MUFA in carp both for wild and farmed carp in all seasons. The PUFA for wild carp was mainly dominated by DHA, while on farmed carp by LA. It confirmed that high LA content in farmed carp was related to the diet habit. The amount of lipid and FA were changed in line with the season. Even the statistical analysis showed no significant difference, but some studies showed a contrasting result. Moreover, most obtained results acknowledged that FA tends to decrease during the spawning period. The amount and composition of FA were affected by the total lipid content. The lipid must be broken down into simpler compounds such as FA or FFA for the metabolism of fish. The result of metabolism then transported into the utilising tissue and used as energy.

  • The zooecological remediation of technogen faulted soil in industrial region of Ukraine steppe zone
    111-115
    Views:
    184

    In Ukraine’s Steppe zone the extraction of minerals is important. To eliminate the consequences of coal mining the agricultural recultivation of the disturbed soil is used. Thus toxic compounds for human beings and the majority of plants and soil biota representatives, which can be found mining rock, get into plants and invertebrates by trophy chains. When remediating soil, it is necessary to create tropic conditions in order to provide the life of soil biota. Earthworms (Lumbricidae) are the primary decomposers of the organic material. They are numerous in soil and facilitate the improvement of natural and artificially created soil. This paper studies the possible influence of different variants of substrates, which are used in re-cultivation, the leaf litter from leaves of different wood species, as well as different levels of humidity on the representatives of soil saprophages. Optimal variants of artificial mixed-soil providing the stable existence of animals have been shown, which are recommended for the implementation of rehabilitation measures.

  • The impact of climatic factors on the relative chlorophyll content and yield of a maize hybrid in a long-term experiment
    71-77
    Views:
    398

    The impact of the climatic factors of crop year on the relative chlorophyll content of maize was examined for three years. The examinations were carried out on the Látókép Experiment Site of the University of Debrecen on calcareous chernozem soil in a small-plot, non-irrigated long-term field experiment with strip plot design. In addition to a non-fertilised (control) treatment, nitrogen (N) fertiliser doses were applied as base and top dressing. The 60 and 120 kg N ha-1 base dressing doses were followed by two top dressing doses at the V6 and V12 phenophases.

    Averaged over the different fertiliser treatments, SPAD readings increased in all three years as the growing season progressed. The highes SPAD value increase was observed in the average crop year (2017) at the V12 phenophase (11.8), which further increased at the R1 phenophas, by 3,7. No significant Spad value difference was observed between the average (2017) and the dry year (2018) at the V6 growth phase. However, in the wet crop year (2016), the V690 treatment provided the statistically highest relative chlorophyll content (46.8). At the V12 phenophase, the base dressing dose of 120 kg N ha-1+30 kg N ha-1 (V6150) showed to be successful in two years (2016 and 2018), while in 2017, the base dressing dose of A60 was successful. The impact of crop year on relative chlorophyll content can be clearly shown at the R1 growth stage. In all three years, the significantly highest relative chlorophyll content could be achieved at different nutrient levels: A60 in 2016, V6150 in 2017 and V690.

    In a wet year (2016), higher yield could be achieved as a result of the 60 kg N ha-1 base dressing and 30 kg N ha-1 at the V6 growth stage (V690) as top dressing in comparison with 2017 and 2018, when higher fertiliser dose (120 kg N ha-1 base dressing and 30 kg N ha-1top dressing at the V6 growth stage) was needed to achieve a significant yield surplus.

    Altogether, averaged over the different treatments, the highest yield (12.48 t ha-1) was observed in the wet year, when the relative chlorophyll content was also the highest (50.6).

  • Effects of paraffine oil on leaf and berry mycobiota on two grape varieties
    61-66
    Views:
    521

    Application of fungicides have advantages and also some direct or indirect disadvantages, such as imbalance and/or fungicide resistance in microbe population. To avoid these problems the development of alternative, eco-friendly methods like mostly spraying with oils are in the focus nowadays. The investigations of the effects of fungicides on microbiota in some cultivations can give a more complex view to this topic and developmental possibilities. In the present study, our aim was testing of the effects of paraffine oil (as alternative fungicide) on microbial properties (CFU and rate of filamentous fungi and yeasts) of Chardonnay and Kékfrankos leaves and berries.

    Our results from 2014 showed that the application of paraffine oil as sole spray agent can decrease the presence of saprophytic filamentous fungi on the berries of Chardonnay (susceptible for fungal infections). In the case of Kékfrankos berries opposite properties were observed, which may be the result of the absorption of oil by the thick wax layer of this variety. The oil treatment did not affect the yeast population of Chardonnay and Kékfrankos berries contrary to negative effect of the regular pesticide treatment. The selective fungicide effect of paraffine oil against filamentous fungal population caused the accumulation of yeast cells in the mycobiota of grape berries. The careful use of this yeasts in spontaneous fermentation can improve the aroma profile of wines. The year of 2015 did no prefer the growth of fungi, therefore no interesting properties were detected in the mycobiota of grape varieties. The occurence of the harmful saprophytic filamentous fungi predicted to be increased in mild climate agricultures as the result of the climate change.

    In summary, the paraffine oils are seem to be promising tools for the eco-friendly control of harmful fungi of grapes.

  • Determination of element contents of commercial and homemade jams
    71-75
    Views:
    664
    Various products could be prepared by using fruits as raw materials. In Hungary, the most popular fruit products are spirit, jams, preserves, juices and dried fruits. Jams are made from different fruits, with different fruit content, technology and additives. They can be used for different products or they can be consumed by themselves. These products and their related production processes have great traditions in Hungary. In addition, they are widely used.
    The aim of our study was to determine the element content of jams and to compare these samples based on their micro- and macroelement content.
    In this study, 26 fruit jams with different botanical origins were examined. 10 samples were commercial, and 16 samples were homemade products. The determination of micro- and macroelements was carried out by using Inductively Coupled Plasma Mass Spectrometer and Inductively Coupled Plasma Optical Emission Spectrometer. Different element contents were measured [B (249.7), Ca (422.6), Fe (259.9), K (769.8), Mg (280.2), Na (589.5), P (185.9), S (182.0), Al (27), Mn (55), Cu (65), Zn (66), Sr (88), Ba (137)]. Based on element contents, the jams showed statistical differences.
  • Additional information for the interpretation of the practice of rural development in the light of the corporate relations of the Agricultural Centre, University of Debrecen in the North-Great Plain Region
    65-69
    Views:
    193

    In the spirit of the Lisbon strategy, the European Union has set out the primary aims of innovation development and competitiveness. A potential method to reach these aims is to enhance the relation between science and practice and to apply scientific results. This is the case at the Agricultural Centre of the University of Debrecen as well, since the tighter and looser cooperations of the institution provide a scientific structure, which is now a significant prerequisite for Hungarian agro-innovation, primarily for rural development processes in the North Great Plain Region. The present study, based mostly on empirical findings, strives to analyze the field of activities in relation to rural development performed by the Agricultural Centre, University of Debrecen.

  • The effect of the supply chain exerting physical stress on the microbiological status of bottled natural mineral water
    189-193
    Views:
    322

    Mineral water consumption increased dramatically in the last 30 years. One reason for this change lies in the changing consumer behaviour: consumers are increasingly recognizing the importance of healthy nutrition and appreciate the beneficial nutritional physiological properties of mineral water. Local mineral water harmonizes well with imported waters. Bottled mineral water may travel several hundred kilometres until it reaches consumers. In the present study, the dynamic mechanical vibration caused by transporting on public roads was simulated in laboratory vibration tests then samples were subjected to microbiological examinations in compliance with legislations currently in force. Due to this vibration, the initial microbe count increased by two orders of magnitude, while after terminating the 4-hour mechanical action it decreased gradually. Growing dynamics of microbes constituting the total germ count at 22 °C and 37 °C were almost similar.

  • SSR based characterization of peach (Prunus persica L.) and apricot (Prunus armeniaca L.) varieties cultivated in Hungary
    17-24
    Views:
    474
    The SSR (Simple Sequence Repeat) markers allow the discrimination of the cultivars and determination its specific DNA fingerprints. The aim of this research was to evaluate fifteen apricot (Prunus armeniaca L.) and fifty-one peach (Prunus persica L.) genotypes cultivated in Hungary to obtain their DNA fingerprints in 6 SSR (Simple Sequence Repeats) loci by allele numbers and sizes.
    DNAs were extracted from leaves. PCR was carried out with CY-5 fluorescent labeled Prunus microsatellite markers and the products were separated on polyacrylamide gel with ALF (Automated Laser Flourometer)-Express II.
    According to our results, in the case of peach genotypes, all 6 SSRs were able to amplify alleles. UDP 96 005 was the most informative marker and UCDCH 17 was the least due to its monomorphic pattern. Regarding the apricot samples BPPCT 041 did not amplify any allele. In the case of P. armeniaca UDP 96 005 had the highest heterozygosity index as well and the highest number of alleles. The least informative marker was the UCDCH 17. Since the 6 SSR were not enough to discriminate the apricot and peach genotypes, it is suggested to use more SSR primers.
  • Biostimulant induce growth, chlorophyll content and fresh herbage yield of alfalfa (Medicago sativa L.) and variegated alfalfa (Medicago × varia Martyn) plant
    19-25
    Views:
    415

    The use of biostimulants is associated with promoting plant growth by stimulating cell division and improving nutrient availability and uptake. A study was conducted at the University of Debrecen, Hungary, to examine the effect of biostimulants on alfalfa growth, chlorophyll content and fresh herbage yield. The experiment was arranged in a randomised complete block design with three biostimulant treatments plus control replicated three times. Data collected were subjected to analyses of variance using Genstat, where significantly different means were separated at a probability of 5% using the least significant difference. The findings show no different variation in plant height or chlorophyll content (SPAD) throughout the early stages of growth. Nonetheless, a notable impact was noted in the latter stages (28 days after biostimulant treatment application) on the growth of the alfalfa plant. Biostimulant treatments did not had effect on fresh yield for second through fourth cuts, but the fifth cut showed a significant effect, with T1 treatment recording the highest herbage yield of 19745 kg ha-1 followed by T2 (Tricho Immun plus Ino Green) and T3 (Tricho Immun), with yields of 19528 kg ha-1 and 17273 kg ha-1, respectively, while the T0 (control) recorded the lowest herbage yield of 12060 kg ha-1. However, the average mean yield indicated the application of biostimulants significantly increased fresh yield herbage by 20.5%. Correlation coefficient values suggested plant height at both 14 and 28 DAH (days after harvest) strongly correlated with fresh herbage yield (r = 0.7756 and 0.7455) which reflected in the increase in fresh herbage yield. Therefore, our results suggest that the use of biostimulants in alfalfa cultivation holds promise for improving growth and yield potential through their positive effects on chlorophyll content and the growth of alfalfa plant.