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Baking quality of winter wheat (Triticum aestivum L.) in the long-term experiments on chernozem soil
152-156Views:100Agriculture has traditionally an important role in Hungarian economy and rural development. About 75 % of Hungary’s total territory
is under agricultural land use. Because of ecological conditions and production traditions cereals (wheat, maize etc) have the greatest
importance in Hungarian crop production. In the 1980’s the country-average yields of wheat were about 5,0-5,5 t ha-1 („industrial-like”
crop production-model). In the 1990’s the yields of wheat dropped to 4,0 t ha-1 because of low input-using and wide application of the issues
of environmental protection and sustainability. Winter wheat production for quality has a decisive role in certain regions of Hungary
(eastern and middle-parts).
The quality of wheat is complex and different. Three major growing factor groups determine the quality of winter wheat: genotype,
agroecological conditions and agrotechnical factors. In wheat production for quality the selection of the variety is the most important
element. Our long-term experiments proved that the quality traits of a variety means the highest (maximum) limit of quality which could not
be exceeded in fact. During the vegetation period of wheat the different ecological and agrotechnical factors could help or on the contrary
could demage the quality parameters of wheat.
The agrotechnical factors determining the baking quality of wheat can be divided into two groups: the first group means the factors with
direct effects on quality (fertilization, irrigation, harvest); the second group contains the elements with indirect effects on quality (crop
rotation, tillage, planting, crop protection).
Appropriate fertilization could help to manifest the maximum of quality parameters of a wheat genotype and could reduce the qualityfluctuation
in unfavourable ecological and agrotechnical conditions. -
The role of the hybrid-specific technological recommendations of maize in precision crop production procedures
297-302Views:118The necessity of application of hybrid specific crop production technology has been confirmed not only by trial results but also by the experiences gained from the agricultural practice. For this reason it is essential to test and collect data in field trials about the specific agronomic traits of the corn hybrids belonging to different maturity groups and genotypes. Corn hybrids are tested for their responses to sowing time, plant density and fertilizer supply; sensitivity to herbicides; and lately, the resistance to the damages caused by the larvae of corn root worm. Last but not least, mention should be made of the differences in the responses of the corn hybrids to the damages caused by drought stress. Based on the trial results, suggestions for the hybrid specific corn production are compiled and made public for the experts and farmers engaged in corn growing. Corn hybrids may deliver maximum yields on the impact of specific crop production technology only in case if it relies on carefully done general production technology including soil cultivation, seed bed preparation and weed control. Similarly, precision crop production technology may advance the yield increase in economic way if it is constantly drawing on the source of research results.
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Examination the effects of different herbicides on the soil microorganisms of a calcareous chernozem
121-126Views:91Pesticides play a key role in fighting weeds, pests and parasitic fungi. According to surveys, pests reduce the yield of agricultural crops by 35% worldwide. Pests, fungi and weeds account for 14%, 12% and 9% yield loss, respectively (Gáborjányi et al., 1995). Chemicals have contributed to increasing and maintaining the yields of crop production for decades. Today, agricultural production (in spite of many efforts) is unthinkable without the use of pesticides (herbicides, insecticides and fungicides). On the other hand, these chemicals contribute to the pollution of the atmosphere, surface and underground waters, and agricultural soils, especially if they are applied improperly.
The sustainable agricultural production pays attention to environment-friendly cultivation-technologies; but at the same time it makes an effort to produce good quality and economical products. The examination of the herbicides’ secondary effects, fits into this chain of idas namely, how the herbicides affect – stimulating or inhibiting – the soil microbiological processes, prevention of soil fertility.
In the course of the experimental work the effect of herbicides on soil biological properties were examined in different maize (Zea mays) cultures. We wanted wished to know that how the herbicides affect the quantity change of soil microorganisms, the life of different physiological groups of bacteria and the activity of microorganisms. A small pot experiment was set up in 2008 with the application of two herbicides - Acenit A 880 EC and Merlin 480 SC – in the breeding house of the Department. The moisture content and nutrient supply were at optimal level in the experiment.
On the basis of results the following can be stated:
1. It can be stated that the two herbicides and all their doses affected negatively the number of total soil bacteria, the
inhibiting effects were significant. The quantity of microscopical fungi increased by the effect of Merlin 480 SC and decreased in the treatments of Acenit A 880 EC.
2. The Acenit A 880 EC had stimulating effect on the nitrate mobilization. The CO2-production was stimulated by the basic doses of herbicides; the other treatments did not influence the CO2-production significantly.
3. The quantity of microbial biomass-carbon –except for only one treatment- decreased significantly by the effect of herbicides. Besides it, the quantity of microbial biomass-nitrogen increased significantly in the treatments of Acenit A 880 EC.
4. The biomass of test plant decreased in the treatments of herbicides, their quantities were smaller than in the control. In the pots treated by Merlin 480 SC, parallel with the increase of doses decreased the quantity of plant-biomass. -
Development of technology elements for growing of perennial sorghum
15-17Views:137Optimal sowing time for perennial sorghum under irrigation is when soil temperature at the depth of sowing reaches up to 10–11 °С, harvesting for the green mass has to be done when panicle is situated on the stem in 10 sm from the flag leaf and hight of cut must be 11 sm.
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Studies on the Fusarium stalk rot infection of the maize genotypes using the Findex percentage and a computerised image analysis program
45-51Views:115In a continental climate, the pathogens causing the most serious problems are species belonging to the Fusarium genus. When the pathogen attacks the stalk, the plant dies earlier, reducing grain filling and resulting in small, light ears. In addition, the stalks break or lodge, resulting in further yield losses from ears that cannot be harvested. During the three years of the experiment, 14 inbred lines were examined. The genotypes were sown in a two-factor split-plot design with four replications, with the genotypes in the main plots and four treatments in the subplots: two Fusarium graminearum isolates (1. FG36, 2. FGH4), 3. sterile kernels, 4. untreated control. The results experiments showed significant differences between the genotypes for resistance to fusarium stalk rot. Among the inbred lines the best resistance to fusarium stalk rot was exhibited by P06 and P07, both of which were related to ISSS. The precision and sensitivity of disease evaluations carried out visually and using image analysis software were compared in the experiment, and with two exceptions the CV values were lower for the image analysis. As the CV for measurements can be considered as a relative error, it can be stated that image analysis is the more precise of the two methods, so this technique gives a more accurate picture of the extent of stalk rot. The extent of stalk rot developing in response to natural infection is extremely environment-dependent, so the use of artificial inoculation is recommended for selection trials.
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Caliometric characterisation of crop production byproducts
55-58Views:158By the decreasing tendency of the fossil energy resources more emphasis put on the usage of renewable energy resources. The consideration of environmental protection and the efforts of the European Union make current the widespread usage of renewable energy within biomass energy. One of the determinative trends of biomass energy is the direct combustion of biomass. Characteristically woody stocks are produced for this aim, but there is a considerable potential in several byproducts of growing herbaceous plants or of other processes.
In our study three byproducts of plant production have been investigated which appeared at the harvest. The Higher Heating Values of wheat, maize and sunflower byproducts have been determined by an IKA C2000 Basic adiabatic calorimeter. According to the statistical analysis of the measured data the HHV of the byproducts are different, and these values are in a negative correlation with the amount of ash in % (R2=0.873) appeared by the combustion.
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Genetic progress in winter wheat quality and quantity parameters
71-75Views:173Wheat production is significant branch of Hungarian crop production (with about 1 million hectares of sowing area). Weather anomalies resulted by climate change have increased the importance of biological basis in wheat production. Yield quality and quantity parameters of three wheat genotypes sown on chernozem soil type after maize pre-crop were studied in a long-term field experiment. Yield amount of the studied genotypes varied between 2894 and 8074 kg ha-1 in 2017 and between 5795 and 9547 kg ha-1 in 2018 depending on the applied treatments. Based on our results it can be stated that in both studied crop years the highest yield increment was realized by the application of the nutrient supply level of N30+PK. As the result of the application of the optimum mineral fertilizer level – in contrast to the control – resulted in significant yield increment in both crop years. The results of the long-term field experiment prove that water utilization of the studied wheat varieties / hybrids was improved by the application of the optimal nutrient supply. Furthermore, the water utilization of the latest genotypes was more favorable by both the control and the optimum nutrient supply level treatments. Analyzing the quality parameters of winter wheat using the NIR method it has been stated that the quality results of the well-known genotype (GK Öthalom) were better than those of the new genotypes. A negative correlation between winter wheat quality and quantity parameters has also been confirmed. As the result of the mineral fertilizer application protein and gluten content of winter wheat increased to a significant extent.
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Name Brands and Symbols Used Organic Products in the European Union and in Hungary
174-180Views:106In 2002, the area which was monitored according to the requirements of organic farming numbered 103 thousand hectares in Hungary (this is some 1.7% of all cultivated land) and almost 1000 plants producing organic products were inspected. It is a realistic assumption, when considering these data, that within a short period time the area used for organic farming will reach the optimal 600.000 hectares.
Contrary to overproduction in the EU – cereals, maize and other plants – organic products can be sold in unlimited quantities. Practice also indicates, that 90% of Hungarian certified organic products are sold in EU and Swiss markets.
Thus, it is important to label Hungarian products according to EU standards. This way standard quality products can be sold easier and by increasing their income, manufacturers can contribute to the improvement of the environment. -
Detailed specification of the steps of dry milling ethanol production
123-126Views:145Durring the 2011 year I was given the possibilty to study in Indiana, USA for 5 months with the help of the Bloomington fellowship, and had the chance to study the bioethanol production in the given state. I focused mainly on the details of corn based dry milling large scale bioethanol production. The dry milling process is a relatively common production mode in the USA. In the coure of my research I tried to compare and to highlite the advantages of the dry milling process contrasted with the wet milling bioethanol production.
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Economic questions of precision maize production on chernozem soil
293-296Views:132It is one of the main topical objective to establish the conditions of sustainable farming. The sustainable development in crop production also calls for the harmony of satisfying human needs and providing the protection of environmental and natural resources; therefore, the maximum consideratio of production site endowments, the common implementation of production needs and environmental protection aims, the minimum load on the environment and economicalness. Precision farmin encompasses the farming method which is adjusted to the given production site, the changing technology in a given plot, the integrated crop protection, cutting edge technologies, remote sensing, GIS, geostatistics, the change
of the mechanisation of crop production, and the application of information technology novelties in crop production. Modern technology increases efficiency and reduces costs. The efficiency of crop production increases by reducing losses and the farmer has access to a better decision support information technology system. In addition, we consider it necessary to examine the two currently most important economic issues: “is it worth it?” and “how much does it cost?”. During the analysis of agricultural technologies, we used the precision crop production experiment database of KITE Zrt. and the Institute for Land Utilisation, Regional Development and Technology of the Centre for Agricultural and Applied Economic Sciences of the University of Debrecen.
During our analytical work, we examined three technological alternatives on two soil types (chernozem and meadow). The first technology is the currently used autumn ploughing cultivation. We extended our analyses to the economic evaluation of satellite navigationassisted ploughing and strip till systems which prefer moisture saving. On chernozem soil, of the satellite-based technological alternatives, the autumn ploughing cultivation provided higher income than strip till. In years with average precipitation supply, we recommend the precision autumn ploughing technological alternative on chernozem soils in the future. On meadow soil, the strip till cultivation technology has more favourable economical results than the autumn ploughing. On soils with high plasticity – considering the high time and energy demand of cultivation and the short amoung of time available for cultivation – we recommend to use strip till technologies. -
The role of agriculture in regional development
25-30Views:260Agriculture is the leading national economic sector of Hajdú-Bihar county. The proportion of employees in this sector and the share of pro ducts produced in this region is above the country average. The contribution of agriculture to the GDP is 6.3%, as opposed to the average value of the country (3.3%). Based on their primary activity, 8% of the enterprises in the county are classified as agricultural and forestry companies, while this proportion is only 5.3% in the whole country.
Nearly three quarters (71.1%) of the sowing area is occupied by maize and other cereals, mainly wheat. This proportion is slightly more than the country average (69.2%). The proportional area of oil crops - including sunflower – and roughage is nearly the same as the country average. However, there is a significant difference in vegetable production. Due to the higher intensity of vegetable production, the area occupied by vegetables is three times the country average. 19.4% of the crop products, 23.5% of animal products and living animals, 25.8% of the income generated by production factors and 29.8% of net enterprise income of Hungary originates from the North Great Plain region.
The facilitation of creating local markets, e.g. local public food supply, satisfying the needs of food trade with local goods, the necessary awareness raising actions, secondary and higher education of experts, as well as the more specific and targeted utilisation of development resources are agricultural development tasks.
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Comparative analysis of certain soil microbiological characteristics of the carbon cycle
137-141Views:150In our researches, we examine the soil microbial parameters related to the carbon cycle. In this study, we compare the changes of microbial biomass carbon (MBC) and the soil CO2 production in soil samples which were taken in spring and autumn. The 30 years old long-term experiment of Debrecen-Látókép is continued in our experiments. The long-term fertilization experiment was set in 1983, and our sample was taken in spring 2014. The examinations of soil respiration processes and factors that influence soil respiration are required in optimal management. In our study, we interested to know how the growing levels of fertilization influence the soil respiration and microbial biomass carbon under non-irrigated and irrigated conditions in maize mono, bi, and triculture.
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Data on the Orthoptera fauna of characteristic agricultural landscape in the Carpathian Lowland
25-34Views:187Orthoptera fauna and assemblages of natural and semi-natural grasslands of the Hungarian Lowland are well-known, however, little is known about assemblages living in agricultural and anthropogenic habitats such as arable lands, roadsides, hedges, and riverbanks. Due to climate change, intensification of agriculture, and change of habitat use, these habitat types become increasingly important.
To collect data on these mainly unknown habitat types, a three-year study was carried out on the Orthoptera fauna and assemblages of the firth region of the Tisza and Sajó rivers. This area was mainly unknown, and our research contributes to increasing knowledge and provides a base for further investigations.
In the 40 sampling sites of the studied region, an occurrence of 30 Orthoptera species was recorded based on 2241 sampled individuals. In this study, we provide 540 new distribution data records of orthopterans that means an almost eightfold increase of the known data. Orthoptera assemblages of different agricultural habitat types showed significant differences considering both species richness and composition. Data suggested that non-cultivated habitat patches of dirt-roads, roadsides and stubble fields and even extensively used pastures, hayfields and alfalfa, red clover, and even wheat fields can preserve relatively species-rich Orthoptera assemblages. Contrary weedy sites of these cultivars and intensively used arable lands (maize, sunflower and rape fields) showed extremely low species diversity.
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The role and impact of N-Lock (N-stabilizer) to the utilization of N in the main arable crops
51-55Views:236The nitrogen stabilizer called N-Lock can be used primarily with solid and liquid urea, UAN and other liquid nitrogen, slurry and manure. In corn it can be applied incorporated before sowing or with row-cultivator or applied with postemergent timing in tank-mix. In postemergent timing need precipitation for long effect. In oil seed rape and autumn cereals the N-Lock should be applied with liquid nitrogen in tank mix late winter or early spring (February-March). The dose rate is 2.5 l/ha. N-Lock increases the yield of maize, winter oil seed rape, winter wheat and winter barley 5-20 %. The yield increasing can be given the thousand grain weight. In case of high doses of nitrogen it can be observed higher yield. The quality parameter also improved, especially the oil content of winter oil seed rape and protein and gluten contents of winter wheat. The use of N-Lock increases the nitrogen retention of soil and reduces nitrate leaching towards the groundwater and the greenhouse effect gas emissions into the atmosphere. The degradation of the applied nitrogen is slowing down and the plant can uptake more nitrogen in long period. The effect of N-Lock the nitrogen is located in the upper soil layer of 0-30 cm and increasing the ammonium nitrogen form. The product can be mixed with herbicide products in main arable crops.
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The Effect of Drought on the Yield of Winter Barley Lines in the Great Cumania Region
127-129Views:79Winter barley is the third most important fodder plant in Hungary after winter wheat and maize.
Its accommodation to the changing climatic conditions – mainly to drought in Great Cumania, the driest region of our country is of great importance from the point of view of plant breeding and growing.
The vegetation period of 2002/2003 can be considered to have been very droughty with 247.5 mm precipitation.
According to our results six- rowed barley lines have better drought tolerance than two-rowed lines. The average yields of the six-rowed barley lines were 12-25 per cent higher than the yields of the two rowed lines.
Due to the joint effect of delayed sowing and drought, significant yield depression was detected both in the case of the early and the medium maturity group, and assessable data could be gained only from 35 per cent of the total territory. -
Effect of mineral fertilizers with different sulphur content on the yield of winter wheat in a small plot experiment
97-100Views:153We can find more and more references on the importance of sulphur and sulphur fertilization, mainly in the case of most important field crops, as cereals, maize, and the oilseeds. The traditional sulphur sources continuously lost their importance by the stringent air pollution orders and nowadays they are not able to meet the demands of plants. Besides, the application of superphosphate, as phosphorus mineral fertilizer, decreased in several countries. Sulphur fertilization is required in most cases to reach the required yields and quality parameters. The lack of
sulphur causes decreased nitrogen utilization and yield by cereals as winter wheat, moreover, results less favourable baking parameters.
We have examined the N and S content of different parts of winter wheat plants in a small plot experiment at the University of Debrecen, Centre of Agricultural Sciences, Látókép Experimental Station. We have also studied the effect of different sulphur fertilization forms and doses on the baking quality parameters and yield. Our aim was to contribute to the development of yield and quality improving sulphur fertilization methods, adaptable by practice.
We have measured the lowest yield in the case of one of the control plots, and 100 kg/ha nitrogen, 80 kg/ha potassium and 70 kg/ha phosphorus nutrient supply resulted the lowest yield (8.7 kg/plot). The maximum yield, 10.2 kg/plot was also experienced on superphosphate fertilized plot by the application of 140 kg/ha phosphorus dose. Application the first dose of both Biofert and FitoHorm 32 S leaf fertilizers raised the yield from
9.1 to 9.9 kg/plot. ANOVA proved that Biofert and FitoHorm 32 S had no significant effect on the amount of yield, the increase is only a tendency. -
Phosphorus (from Different Phosphorus Sources) Utilisation in Piglets, and the Effect of the Addition of Phytase into the Feedstuff
30-36Views:82The experiments were carried out in a 2x2 factorial treatments with three replicates, and were completed with 32P phosphorus metabolism measurement. Hungarian Large White x Dutch Landrace growing pigs with 15–18 kg starting live weight were involved in the experiment.
The experimental scheme was the following:
Diet consisted of maize and extracted soybean meal. Both components have high phytase content and low phytase activity. 1/a animals received their P-supply according to their needs and 1/b animals got 10% less than their actual P-need in the first part of the experiment.
In the second part of the experiment both groups (2/a, 2/b) received identical P-supply and 500FTU/kg P supplementation. Apart from P- and phytase-supplementation, the piglets’ diet was identical.
Total P digestibility was 52% without phytase supplementation, which increases by 4% when P was added according to need and by 12% increase of decreased P-supply. Digestibility of nutrients somewhat increased as effect of phytase supplementation. According to the results of 32P experiments, inorganic P digestibility of MCP was 82–90.8%, which decreases to 73.4–87.2% in case of phytase supplementation.
Parallel with tendency, native P digestibility of the diet was 31.5–32.2%, which increased to 42.5–54.5% in the case of phytase supplementation.
Results support the that inorganic P input can be decreased by phytase supplementation and as a consequence P output, the concept and environmental pollution can at the some time be decreased. -
Risk effects of the spread route of mycotoxins
90-95Views:163In Hungary the mycotoxin is a great problem, because there are many natural toxins in wheat and maize. These cereals can be found on
considerable proportion of the country’s sowing area, and they are deterministic food for the population. The direct human and animal
utilization of the contaminated cereals mean a serious risk in the food chain. In Hungary’s climate the soil is contaminated with pathogen
moulds, particularly Fusarium species, which increase by respective temperature and moisture content in cereals. The Fusarium can
decrease the quality of the wheat in different ways: decrease the germination capability and cause visible discoloration and appearance of
mould, reduces the dry material and nutrient content of the grain. From the toxins produced by the Fusarium genus, the trichotecene (T-2,
HT-2, deoxinivalenol, nivalenol, diacetoxyscxirpenol, Fusarenon-X) and the estrogenic zearalenon (F-2) are the most common in Hungary.
The fumonisins (FB1, FB2, FB3) first identified in 1988, relatively newly discovered, are also important. Major proportion of mycotoxins in a
healthy organization is metabolized by the enzyme system of liver and intestinal bacteria. The toxicity is reduced or even leaves off.
However, more toxic and biologically active compounds can be formed. For the reduction of mycotoxin-contamination several possibilities
are available in the case of storage, processing and feeding. -
Evaluation of long term experiments from a new aspect
55-60Views:132During our work, we developed a new, simple method to show the effects of fertilization on yield, which can both be applied over the long term as well as in series of independent experiments.
During the testing of this method, at the experimental farm of the Debrecen University Center for Agricultural Sciences at Látókép on a chernozem soil with lime deposits, we examined the fertilizer reaction of maize hybrids between 1989 and 1994. The treatments were: winter tillage, plant density of 70-80 thousand, unfertilized, N 120, N 240 kg/ha fertilized treatments, long term experiments using Dekalb 524 and Volga SC hybrids in long term experiments.
Four parameters are shown in the model. In the examined period TRmax represents the greatest yield in the fertilized treatments, NT the yield in unfertilized treatment, k the „efficiency of fertilizer” to NT and b the depression-coefficient, where the expected value is zero. The expected grain yield of the fertilized treatments (Y), in the function of the unfertilized grain yield (x) is the following:
The parameters were determined using the Monte Carlo method, in the optimizing process the sum of deviation square was minimized. The correct conformation of the functions was determined by the greatness of the R-value and the standard error. We found that during six years of testing, the tendency of fertilization efficiency was similar in the case of both hybrids. There was an unfavorable weather interval and, in these years, the yields were low, fertilization did not have an effect and moreover, in extremely bad conditions resulted in an obvious yield decrease. With the improvement of conditions, which in the case of our country means an increase in precipitation, the efficiency of fertilization increases and reaches its peak at 13-14 t/ha. At this point, the yield increasing effect of fertilization is 4-4,5 t/ha. If the yield of the unfertilized treatments increases from 8-9 t/ha, then the efficiency of the applied fertilizer decreases.
Most likely, the k and b parameters depend on the soil of the experimental location (nutrient and water management) and on the amount of pplied fertilizer and the characteristics of the hybrid. With the increase of fertilizer dosage the k-parameter also increases. The greater value though does not obviously mean a more favorable situation. It is true that in medium and good years this means great fertilizer efficiency, but in low or extreme precipitation conditions it also means greater risk. With the increase of the k-parameter, the yield deviation also increases which, from a cultivation point of view, is quite unfavorable. If the value of the b-parameter is other than, zero then the effect is clearly unfavorable, because with the increase of this value, the yield decrease is also greater. The fertilizer reaction of the two examined hybrids can be well characterized by these two hybrids.
Examining the six years, our created model estimated the effect of fertilization on the yield accurately and with a high degree of safety. Both in highly unfavorable and extremely good years, it gave an exact estimate. In our opinion, it can be used well to evaluate the effects of fertilization on yield in the future. -
Allelopathic Effect of Italian Cocklebur on Sugar Beet
74-77Views:120Cockleburs are noxious weeds in Hungary, where they are widespread in row crops, especially in maize, sunflower and sugarbeet. A low density population of these weeds may be harmful because of their large competitive ability, fast growth in early phenological stages, allelopathy, and persistant sprouting.
Allelopathy of Xanthium italicum Mor. was examined during the growing season in 2004. Root and shoot samples were collected at 4 or 5 leaves stage (in the end of May and in the beginning of June) and before flowering (in the beginning of July) before and after rain. Extracts were made in tap water. The test plant was Beta vulgaris L.; its germination, root and shoot growth were evaluated at the 6th and 10th days after treatment.
Most of extracts inhibited the germination of sugar beet. In these experiments the phenological stage of the donor plant determined slightly the effectiveness of the extracts on sugar beet. Generally, significant differences existed between treatments only in cases of concentrated extracts.
Rainfall can modify the inhibitory effect of extracts (especially shoot extracts). Shoot extracts of young plants inhibited stronger germination and growth after rain than before rain. Density of cockleburs influenced the effect of extracts, as well. Generally, differences between the efficacy of extracts was larger after rain.
The results supported the hypothesis that the phenological stage and some environmental factors can modify allelopathy of cockleburs. -
Qualitative detection of genetically modified organisms in plant samples
309-313Views:178We analysed the GMO content of corn samples by polymerase chain reaction following the appropriate optimization of the reaction. The analysis included two main steps: extraction of DNA from the sample, and detection of the GMO content by polymerase chain reaction. The polymerase chain reaction is an in vitro method to multiply chromosomatic or cloned DNA (cDNA) sequences through the enzymatic pathway. The reaction is sensitive enough to produce DNA in sufficient amount for the analysis from a single DNA. We identified the PCR products by agarose gel electrophoresis. When optimizing the reaction, the MgCl2 concentration, reaction time and temperature have to be taken into consideration. The temperature of the anellation has to be increased until the highest specificity and yield is reached. If the temperature of the anellation is too high, the primer is linked to non-specific sites as well; in the gel visualization, more lines can be seen at one sample. If the temperature of the anellation is too high, the primer is insufficiently linked or is not linked at all (too few lines in the gel visualization). After optimization, the GMO content in the unknown sample can be determined along with the appropriate positive and negative controls.
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Evaluation of the microbial soil quality indicators in agricultural soils from Crisurilor Plain
79-80Views:126The researches were carried out in 2010 and 2011 on the haplic luvisol cultivated in three variant such as: pasture, cropland, and orchards. Based on the total number of microorganisms monitored in the haplic luvisol (aerobic mesophilic heterotrophs, yeast and mould, Actinomycetes, nitrogen fixing bacteria and nitrifying bacteria) was calculated the bacterial indicators of soil quality (BISQ) and was appreciate the bacterial potential of the haplic luvisol. Seasonal analyses were carried out, and annual BISQs have been calculated too. The values of the bacterial indicators of the haplic luvisol in different cultivation conditions (pasture, cropland and orchards) indicate a high density of the bacterial groups in 2010 and 2011. In 2010 the values of the BISQs are included between 3.617 (minimum in autumn, in pasture) and 5.458 (maximum, in spring, in pasture). In 2011, the minimum value (3.622) was registered in pasture, in autumn, and the maximum value (4.851) in the haplic luvisol cultivated with maize, in spring. In 2010 and 2011, based on the bacterial indicators of the soil quality values, on the first position is the cropland (4.750, 4.721) followed by orchards (4.615, 3.985) and pasture (4.537, 3.758).
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Challenges and agroecological approaches in crop production
75-89Views:178Never has the need been greater for an ecosystem approach to agriculture. As our global population exceeds 9 billion in the next 30 years, with a concomitant demand for agricultural products, ever more pressure will be placed on our agricultural systems. Meanwhile, climate change is altering the ecological settings in which agriculture is practiced, demanding adaptation. Knowledge generated by long-term research will help to address one of the grand challenges of our time: how to meet sustainably the growing world demand for agricultural products – in a way that minimizes environmental harm and enhances the delivery of a diverse array of ecosystem services.
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Agronomical and economic evaluation of various cultivation systems on meadow soil
103-106Views:122The requirements and objectives of cultivation are in constant change. There are different cultivation aims if the objective is soil protection, the prevention of its moisture content or on areas with different precipitation supply or production site endowments. Based on the experimental database of the Institute for Land Utilisation, Regional Development and Technology of the University of Debrecen, Centre for Agricultural and Applied Economic Sciences and the KITE Plc., the various cultivation systems in Hajdú-Bihar country were examined with maize as indicator plant. The sample area can be found in the outskirts of Biharnagybajom on meadow soil. On the examined plot, spring strip basic cultivation, loosening and autumn ploughing were applied on 15-15-15 ha, respectively. At the time of taking undisturbed soil samples, soil conductivity measurements were also performed with a Penetronik penetrometer. Undisturbed soil samples were taken from each treatment before sowing (on 5th April 2012). The yield obtained in the strip cultivation treatment increased that of the ploughing and the loosening technology. The economic indexes are the most favourable in the strip cultivation.
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Impact assesment of soil conditioners on a high clay content soil
137-141Views:170Our measurements were done in a soil conditioner experiment started in 2014 which was set in conventional tillage system at the Karcag Research Institute where a soil conditioner was used from 2010. Effect of two different soil conditioners on compaction, moisture content of the soil and on CO2-emission was studied. Measurements were done after sowing of maize and millet, and on stubble after harvesting. It can be established that less degree of compaction was characteristic to the soil of the plots treated for several years with the soil conditioner during the vegetation period than in case of untreated plots. Higher CO2-emission values were observed on the plots treated for several years than on the control plots. This effect can’t be established in case of soil conditioners used for first time in this year.